Rh5AG045100

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0008620
Physical Location & Seq
Forward (+)
3771814 .. 3774535
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG045100.1

Sequence Viewer

Length: 579 bp
ATGGGGGATTTAGAGAAGCAAGCAGAGAGGGTTGTGCTGAGCAAGGACGAGGCGGAAGAGGAGGAGATGGAGAGGCTGGTCGGCGGCGGCGGAATGGCGGTGTTGGATTTCGACATGCTCTGCTCCACCGTCGCCTTACAGACCCAGGGCAAGTCCGCCGCCAAGTTCCAGAGCTTTGGTGCCGGAGAAGAAGAGGAAGAAGAAGAAGCAGCTGAGGTTGGCGATTTCGGCGGCGTGTTCCGGATGTGGGAGGGTGAGCTTCTCGATTGGTTCGACGATCGCCGCGTCGCTATTCAGTCGGCTTGCTGTCCGTGCTATACATTTGGGAAGAACATGAAGCGAGCTGGTTTTGGTCCTGGCTTTTTACAGGGAACTCTTCATTTAGTTCTTGTTGCGAGCATCCTTCTCAACTGCATTGCGTTTATTATCACCAAGAAGCGCTGCTTTATATATCTGGTGGTTGCTTTCACCATTACACTAGGAACGTATTTGGGGTACTTCCGTACACAGATAAGAAACAAATTTAACATAAGGGTAATCACTCTCTCTTTGTCTGTCATTGTGTCCATTTCTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

192

Amino Acids

21.28

Weight (kDa)

4.87

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 83 - 178 3.3e-11 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 1 cut(s) 285
AccIII TCCGGA 1 cut(s) 240
AciI CCGC 9 cut(s) 53, 84, 87, 90, 98, 156, 159, 231, 283
AcsI RAATTY 1 cut(s) 521
AfaI GTAC 2 cut(s) 497, 505
AfeI AGCGCT 1 cut(s) 440
AfiI CCNNNNNNNGG 1 cut(s) 247
AjnI CCWGG 2 cut(s) 144, 355
AluBI AGCT 4 cut(s) 174, 212, 259, 344
AluI AGCT 4 cut(s) 174, 212, 259, 344
Aor13HI TCCGGA 1 cut(s) 240
Aor51HI AGCGCT 1 cut(s) 440
ApeKI GCWGC 2 cut(s) 209, 441
ApoI RAATTY 1 cut(s) 521
AspLEI GCGC 1 cut(s) 441
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 3 cut(s) 266, 421, 460
AvaII GGWCC 1 cut(s) 353
BaeI ACNNNNGTAYC 2 cut(s) 487, 520
BanI GGYRCC 1 cut(s) 179
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 221, 428
BccI CCATC 1 cut(s) 61
BciT130I CCWGG 2 cut(s) 146, 357
BfaI CTAG 2 cut(s) 479, 577
BfoI RGCGCY 1 cut(s) 442
BisI GCNGC 7 cut(s) 85, 88, 159, 210, 232, 283, 442
BlpI GCTNAGC 1 cut(s) 38
BlsI GCNGC 7 cut(s) 86, 89, 160, 211, 233, 284, 443
Bme1390I CCNGG 2 cut(s) 146, 357
Bme18I GGWCC 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 2 cut(s) 146, 357
BmsI GCATC 1 cut(s) 408
Bpu10I CCTNAGC 1 cut(s) 213
Bpu1102I GCTNAGC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 144, 145
BsaWI WCCGGW 1 cut(s) 240
Bsc4I CCNNNNNNNGG 1 cut(s) 247
Bse3DI GCAATG 1 cut(s) 414
BseAI TCCGGA 1 cut(s) 240
BseBI CCWGG 2 cut(s) 146, 357
BseDI CCNNGG 2 cut(s) 144, 145
BseGI GGATG 2 cut(s) 249, 399
BseLI CCNNNNNNNGG 1 cut(s) 247
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 2 cut(s) 29, 204
BseRI GAGGAG 2 cut(s) 74, 77
BseXI GCAGC 2 cut(s) 221, 428
Bsh1236I CGCG 1 cut(s) 285
Bsh1285I CGRYCG 1 cut(s) 280
BshNI GGYRCC 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 280
BsiSI CCGG 2 cut(s) 183, 241
BslI CCNNNNNNNGG 1 cut(s) 247
Bsp13I TCCGGA 1 cut(s) 240
Bsp143I GATC 1 cut(s) 277
Bsp1720I GCTNAGC 1 cut(s) 38
BspACI CCGC 9 cut(s) 53, 84, 87, 90, 98, 156, 159, 231, 283
BspCNI CTCAG 2 cut(s) 30, 205
BspEI TCCGGA 1 cut(s) 240
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 181
BspT107I GGYRCC 1 cut(s) 179
BsrDI GCAATG 1 cut(s) 414
BssECI CCNNGG 2 cut(s) 144, 145
BssMI GATC 1 cut(s) 277
Bst2UI CCWGG 2 cut(s) 146, 357
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 3 cut(s) 51, 186, 381
BstC8I GCNNGC 4 cut(s) 21, 304, 342, 397
BstDEI CTNAG 2 cut(s) 38, 213
BstF5I GGATG 2 cut(s) 249, 399
BstFNI CGCG 1 cut(s) 285
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 1 cut(s) 441
BstKTI GATC 1 cut(s) 280
BstMBI GATC 1 cut(s) 277
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 2 cut(s) 228, 312
BstNI CCWGG 2 cut(s) 146, 357
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 2 cut(s) 144, 355
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 2 cut(s) 221, 428
BstXI CCANNNNNNTGG 1 cut(s) 176
BtsCI GGATG 2 cut(s) 249, 399
Cac8I GCNNGC 4 cut(s) 21, 304, 342, 397
CfoI GCGC 1 cut(s) 441
Cfr13I GGNCC 1 cut(s) 353
CseI GACGC 1 cut(s) 274
Csp6I GTAC 2 cut(s) 496, 504
CviAII CATG 2 cut(s) 115, 334
CviJI RGCY 7 cut(s) 76, 174, 212, 259, 302, 344, 360
CviKI_1 RGCY 7 cut(s) 76, 174, 212, 259, 302, 344, 360
CviQI GTAC 2 cut(s) 496, 504
DdeI CTNAG 2 cut(s) 38, 213
DpnI GATC 1 cut(s) 279
DpnII GATC 1 cut(s) 277
Eam1104I CTCTTC 3 cut(s) 51, 186, 381
EarI CTCTTC 3 cut(s) 51, 186, 381
EciI GGCGGA 3 cut(s) 68, 105, 145
Eco47I GGWCC 1 cut(s) 353
Eco47III AGCGCT 1 cut(s) 440
EcoRII CCWGG 2 cut(s) 144, 355
FaeI CATG 2 cut(s) 118, 337
FaiI YATR 6 cut(s) 116, 318, 335, 449, 451, 530
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FatI CATG 2 cut(s) 114, 333
Fnu4HI GCNGC 7 cut(s) 85, 88, 159, 210, 232, 283, 442
FokI GGATG 2 cut(s) 256, 386
Fsp4HI GCNGC 7 cut(s) 85, 88, 159, 210, 232, 283, 442
FspBI CTAG 2 cut(s) 479, 577
GlaI GCGC 1 cut(s) 440
GluI GCNGC 7 cut(s) 85, 88, 159, 210, 232, 283, 442
HaeII RGCGCY 1 cut(s) 442
HapII CCGG 2 cut(s) 183, 241
HgaI GACGC 1 cut(s) 274
HhaI GCGC 1 cut(s) 441
Hin1II CATG 2 cut(s) 118, 337
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HpaII CCGG 2 cut(s) 183, 241
HphI GGTGA 3 cut(s) 266, 421, 460
Hpy166II GTNNAC 1 cut(s) 506
Hpy188III TCNNGA 3 cut(s) 169, 241, 263
Hpy8I GTNNAC 1 cut(s) 506
Hpy99I CGWCG 3 cut(s) 134, 278, 290
HpyAV CCTTC 1 cut(s) 413
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 1 cut(s) 414
HpyF10VI GCNNNNNNNGC 2 cut(s) 228, 312
HpyF3I CTNAG 2 cut(s) 38, 213
HpySE526I ACGT 1 cut(s) 485
Hsp92II CATG 2 cut(s) 118, 337
HspAI GCGC 1 cut(s) 439
Kpn2I TCCGGA 1 cut(s) 240
Kzo9I GATC 1 cut(s) 277
LmnI GCTCC 1 cut(s) 128
Lsp1109I GCAGC 2 cut(s) 221, 428
LweI GCATC 1 cut(s) 408
MaeI CTAG 2 cut(s) 479, 577
MaeII ACGT 1 cut(s) 485
MalI GATC 1 cut(s) 279
MboI GATC 1 cut(s) 277
MboII GAAGA 8 cut(s) 68, 200, 203, 209, 212, 215, 340, 368
MluCI AATT 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 8 cut(s) 21, 43, 52, 55, 66, 187, 208, 244
MroI TCCGGA 1 cut(s) 240
MseI TTAA 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 212
MspI CCGG 2 cut(s) 183, 241
MspR9I CCNGG 2 cut(s) 146, 357
MvaI CCWGG 2 cut(s) 146, 357
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 2 cut(s) 228, 312
NdeII GATC 1 cut(s) 277
NlaIII CATG 2 cut(s) 118, 337
NlaIV GGNNCC 1 cut(s) 181
NspI RCATGY 1 cut(s) 118
PasI CCCWGGG 1 cut(s) 145
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 282
PkrI GCNGC 7 cut(s) 86, 89, 160, 211, 233, 284, 443
Ple19I CGATCG 1 cut(s) 280
Psp6I CCWGG 2 cut(s) 144, 355
PspGI CCWGG 2 cut(s) 144, 355
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 1 cut(s) 353
PvuI CGATCG 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 212
RsaI GTAC 2 cut(s) 497, 505
RsaNI GTAC 2 cut(s) 496, 504
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 7 cut(s) 85, 88, 159, 210, 232, 283, 442
Sau3AI GATC 1 cut(s) 277
Sau96I GGNCC 1 cut(s) 353
ScrFI CCNGG 2 cut(s) 146, 357
SetI ASST 6 cut(s) 176, 214, 219, 261, 346, 488
SfaNI GCATC 1 cut(s) 408
SinI GGWCC 1 cut(s) 353
Sse9I AATT 1 cut(s) 521
SsiI CCGC 9 cut(s) 53, 84, 87, 90, 98, 156, 159, 231, 283
SspMI CTAG 2 cut(s) 479, 577
StyD4I CCNGG 2 cut(s) 144, 355
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 1 cut(s) 488
TaqI TCGA 3 cut(s) 111, 264, 273
TasI AATT 1 cut(s) 521
TauI GCSGC 5 cut(s) 87, 90, 161, 234, 285
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TseI GCWGC 2 cut(s) 209, 441
TspDTI ATGAA 2 cut(s) 350, 368
TspGWI ACGGA 2 cut(s) 300, 491
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 1 cut(s) 521
XceI RCATGY 1 cut(s) 118
XspI CTAG 2 cut(s) 479, 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.