Rh5BG043800

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008620
Physical Location & Seq
Forward (+)
3483713 .. 3486135
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG043800.1

Sequence Viewer

Length: 459 bp
ATGAAGCGAGCTGGTTTTGGTCCTGGCTTTTTACAGGGAACTCTTCATTTAGTTCTTGTTGCGAGCATCCTTCTCAACTGCATTGCGTTTATTATCACCAAGAAGCGCTGCTTTATATATCTGGTGGTTGCTTTCACCATTACACTAGGAACGTATTTGGGGTACTTCCGTACACAGATAAGAAACAAATTTAACATAAGGGGTAATGATAGTTCCATGGATGATTGCATCTACCACCTTGTCTGCCCATGCTGCACTTTATCTCAGGAAGCCAGAACATTAGAGATGAACAATGTCCAAGATGGCACCTGGCATGGCAGGGGCGATTCAATATACATAGGCAGCCTTGGAGAAGGAGGAAAGTCTTTCTTTGAGCTCCAAGCACCTCCTCTTGTGTCAATCAAGTCCCCCGACCTCTGCAACGCGCAAAAAAGTACAGCGACTAGTGCATATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

152

Amino Acids

16.74

Weight (kDa)

8.71

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 32 - 92 8.1e-12 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 305
AccII CGCG 1 cut(s) 425
AcsI RAATTY 1 cut(s) 188
AfaI GTAC 3 cut(s) 164, 172, 436
AfeI AGCGCT 1 cut(s) 107
AgsI TTSAA 1 cut(s) 330
AhlI ACTAGT 1 cut(s) 443
AjnI CCWGG 2 cut(s) 22, 308
AluBI AGCT 2 cut(s) 11, 376
AluI AGCT 2 cut(s) 11, 376
Alw21I GWGCWC 1 cut(s) 378
Aor51HI AGCGCT 1 cut(s) 107
ApeKI GCWGC 3 cut(s) 108, 252, 342
ApoI RAATTY 1 cut(s) 188
AspLEI GCGC 2 cut(s) 108, 427
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 2 cut(s) 88, 127
AvaII GGWCC 1 cut(s) 20
BaeI ACNNNNGTAYC 2 cut(s) 154, 187
BanI GGYRCC 1 cut(s) 305
BanII GRGCYC 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 3 cut(s) 95, 239, 354
BccI CCATC 1 cut(s) 296
BciT130I CCWGG 2 cut(s) 24, 310
BcuI ACTAGT 1 cut(s) 443
BfaI CTAG 2 cut(s) 146, 444
BfoI RGCGCY 1 cut(s) 109
BisI GCNGC 3 cut(s) 109, 253, 343
BlsI GCNGC 3 cut(s) 110, 254, 344
Bme1390I CCNGG 2 cut(s) 24, 310
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 1 cut(s) 307
BmrFI CCNGG 2 cut(s) 24, 310
BmsI GCATC 2 cut(s) 75, 237
BsaJI CCNNGG 2 cut(s) 216, 346
Bse3DI GCAATG 1 cut(s) 81
BseBI CCWGG 2 cut(s) 24, 310
BseDI CCNNGG 2 cut(s) 216, 346
BseGI GGATG 2 cut(s) 66, 226
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 1 cut(s) 278
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 3 cut(s) 95, 239, 354
BsgI GTGCAG 1 cut(s) 238
Bsh1236I CGCG 1 cut(s) 425
BshNI GGYRCC 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 378
BslFI GGGAC 1 cut(s) 391
BsmFI GGGAC 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 378
Bsp19I CCATGG 1 cut(s) 216
BspCNI CTCAG 1 cut(s) 277
BspFNI CGCG 1 cut(s) 425
BspHI TCATGA 1 cut(s) 455
BspLI GGNNCC 1 cut(s) 307
BspT107I GGYRCC 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 81
BssECI CCNNGG 2 cut(s) 216, 346
BssT1I CCWWGG 2 cut(s) 216, 346
Bst2UI CCWGG 2 cut(s) 24, 310
Bst6I CTCTTC 1 cut(s) 48
BstC8I GCNNGC 2 cut(s) 9, 64
BstDEI CTNAG 1 cut(s) 264
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 2 cut(s) 66, 226
BstFNI CGCG 1 cut(s) 425
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 2 cut(s) 108, 427
BstMWI GCNNNNNNNGC 2 cut(s) 252, 446
BstNI CCWGG 2 cut(s) 24, 310
BstSCI CCNGG 2 cut(s) 22, 308
BstUI CGCG 1 cut(s) 425
BstV1I GCAGC 3 cut(s) 95, 239, 354
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 2 cut(s) 66, 226
Cac8I GCNNGC 2 cut(s) 9, 64
CciI TCATGA 1 cut(s) 455
CfoI GCGC 2 cut(s) 108, 427
Cfr13I GGNCC 1 cut(s) 20
Csp6I GTAC 3 cut(s) 163, 171, 435
CviAII CATG 4 cut(s) 217, 249, 314, 456
CviJI RGCY 5 cut(s) 11, 27, 272, 345, 376
CviKI_1 RGCY 5 cut(s) 11, 27, 272, 345, 376
CviQI GTAC 3 cut(s) 163, 171, 435
DdeI CTNAG 1 cut(s) 264
Eam1104I CTCTTC 1 cut(s) 48
EarI CTCTTC 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 376
Eco130I CCWWGG 2 cut(s) 216, 346
Eco24I GRGCYC 1 cut(s) 378
Eco47I GGWCC 1 cut(s) 20
Eco47III AGCGCT 1 cut(s) 107
Eco53kI GAGCTC 1 cut(s) 376
EcoICRI GAGCTC 1 cut(s) 376
EcoRII CCWGG 2 cut(s) 22, 308
EcoT14I CCWWGG 2 cut(s) 216, 346
EcoT38I GRGCYC 1 cut(s) 378
ErhI CCWWGG 2 cut(s) 216, 346
FaeI CATG 4 cut(s) 220, 252, 317, 459
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 353, 385
FaqI GGGAC 1 cut(s) 391
FatI CATG 4 cut(s) 216, 248, 313, 455
Fnu4HI GCNGC 3 cut(s) 109, 253, 343
FokI GGATG 2 cut(s) 53, 233
FriOI GRGCYC 1 cut(s) 378
Fsp4HI GCNGC 3 cut(s) 109, 253, 343
FspBI CTAG 2 cut(s) 146, 444
GlaI GCGC 2 cut(s) 107, 426
GluI GCNGC 3 cut(s) 109, 253, 343
HaeII RGCGCY 1 cut(s) 109
HhaI GCGC 2 cut(s) 108, 427
Hin1II CATG 4 cut(s) 220, 252, 317, 459
Hin6I GCGC 2 cut(s) 106, 425
HinP1I GCGC 2 cut(s) 106, 425
HinfI GANTC 1 cut(s) 326
HphI GGTGA 2 cut(s) 88, 127
Hpy166II GTNNAC 1 cut(s) 173
Hpy188III TCNNGA 2 cut(s) 266, 456
Hpy8I GTNNAC 1 cut(s) 173
HpyAV CCTTC 2 cut(s) 80, 347
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 5 cut(s) 81, 228, 255, 420, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 252, 446
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 1 cut(s) 152
Hsp92II CATG 4 cut(s) 220, 252, 317, 459
HspAI GCGC 2 cut(s) 106, 425
LmnI GCTCC 1 cut(s) 381
LpnPI CCDG 9 cut(s) 9, 20, 36, 107, 251, 286, 295, 304, 322
Lsp1109I GCAGC 3 cut(s) 95, 239, 354
LweI GCATC 2 cut(s) 75, 237
MaeI CTAG 2 cut(s) 146, 444
MaeII ACGT 1 cut(s) 152
MboII GAAGA 1 cut(s) 35
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 1 cut(s) 188
MnlI CCTC 4 cut(s) 350, 396, 399, 425
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 454
MspR9I CCNGG 2 cut(s) 24, 310
MvaI CCWGG 2 cut(s) 24, 310
MvnI CGCG 1 cut(s) 425
MwoI GCNNNNNNNGC 2 cut(s) 252, 446
NcoI CCATGG 1 cut(s) 216
NlaIII CATG 4 cut(s) 220, 252, 317, 459
NlaIV GGNNCC 1 cut(s) 307
PagI TCATGA 1 cut(s) 455
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 3 cut(s) 110, 254, 344
Psp124BI GAGCTC 1 cut(s) 378
Psp6I CCWGG 2 cut(s) 22, 308
PspGI CCWGG 2 cut(s) 22, 308
PspN4I GGNNCC 1 cut(s) 307
PspPI GGNCC 1 cut(s) 20
PsrI GAACNNNNNNTAC 2 cut(s) 196, 228
RsaI GTAC 3 cut(s) 164, 172, 436
RsaNI GTAC 3 cut(s) 163, 171, 435
RseI CAYNNNNRTG 1 cut(s) 454
SacI GAGCTC 1 cut(s) 378
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 3 cut(s) 109, 253, 343
Sau96I GGNCC 1 cut(s) 20
ScrFI CCNGG 2 cut(s) 24, 310
SduI GDGCHC 1 cut(s) 378
SetI ASST 7 cut(s) 13, 155, 240, 311, 378, 388, 417
SfaNI GCATC 2 cut(s) 75, 237
SinI GGWCC 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 454
SpeI ACTAGT 1 cut(s) 443
Sse9I AATT 1 cut(s) 188
SspMI CTAG 2 cut(s) 146, 444
SstI GAGCTC 1 cut(s) 378
StyD4I CCNGG 2 cut(s) 22, 308
StyI CCWWGG 2 cut(s) 216, 346
TaiI ACGT 1 cut(s) 155
TasI AATT 1 cut(s) 188
TatI WGTACW 1 cut(s) 434
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 3 cut(s) 108, 252, 342
TspDTI ATGAA 4 cut(s) 17, 35, 302, 444
TspGWI ACGGA 1 cut(s) 158
VpaK11BI GGWCC 1 cut(s) 20
XapI RAATTY 1 cut(s) 188
XspI CTAG 2 cut(s) 146, 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.