Rh5DG043600

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008620
Physical Location & Seq
Forward (+)
3624546 .. 3627135
2590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG043600.1

Sequence Viewer

Length: 570 bp
ATGGGGGATTTAGAGAAGCAAGAGAGGGTTGTGCTGAACAAGGACGAGGCGGAAGAGGAGGAGATGGAGAGGCTGGTCGGCGGCGGCGGCGGCGGAATGGCGGTGTTGGATTTCGACATGCTCTGCTCCACCGTCGCCTTACAGACCCAGGGCAAGTCCGCCGCCAAGTTCCAGAGCTTTGGTGCCGGAGAAGAAGAAGAAGAAGAAGTTGGCGATTTCGGCGGCGTGTTCCGGATGTGGGAGGGTGAGCTTCTCGATTGGTTCGACGATCGCCGCGTCGCTATTCAGTCGGCTTGCTGTCCGTGCTATACATTTGGGAAGAACATGAAGCGAGCCGGTTTTGGTCCTGGCTTTTTACAGGGAACTCTTCATTTAGTTCTTGTTGCGAGCATCCTTCTCAACTGCATTGCGTTTATTATCACCAAGAAGCGCTGCTTTATATATCTGGTGGTTGCTTTCACCATTACACTAGGAACGTATTTGGGGTACTTCCGTACACAGATAAGAAACAAATTTAACATAAGGGTAATCACTCTCTCTTTGTCTGTCGTTGTGTCCATTGCTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

189

Amino Acids

20.92

Weight (kDa)

5.03

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 80 - 175 2.9e-11 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 182
AccII CGCG 1 cut(s) 276
AccIII TCCGGA 1 cut(s) 231
AcsI RAATTY 1 cut(s) 512
AfaI GTAC 2 cut(s) 488, 496
AfeI AGCGCT 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 238
AjnI CCWGG 2 cut(s) 147, 346
AjuI GAANNNNNNNTTGG 2 cut(s) 192, 224
AluBI AGCT 2 cut(s) 177, 250
AluI AGCT 2 cut(s) 177, 250
Aor13HI TCCGGA 1 cut(s) 231
Aor51HI AGCGCT 1 cut(s) 431
ApeKI GCWGC 1 cut(s) 432
ApoI RAATTY 1 cut(s) 512
AspLEI GCGC 1 cut(s) 432
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 3 cut(s) 257, 412, 451
AvaII GGWCC 1 cut(s) 344
BaeI ACNNNNGTAYC 2 cut(s) 478, 511
BanI GGYRCC 1 cut(s) 182
BbvI GCAGC 1 cut(s) 419
BccI CCATC 1 cut(s) 58
BciT130I CCWGG 2 cut(s) 149, 348
BfaI CTAG 2 cut(s) 470, 568
BfoI RGCGCY 1 cut(s) 433
BisI GCNGC 8 cut(s) 82, 85, 88, 91, 162, 223, 274, 433
BlsI GCNGC 8 cut(s) 83, 86, 89, 92, 163, 224, 275, 434
Bme1390I CCNGG 2 cut(s) 149, 348
Bme18I GGWCC 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 2 cut(s) 149, 348
BmsI GCATC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 147, 148
BsaWI WCCGGW 1 cut(s) 231
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse118I RCCGGY 1 cut(s) 335
Bse3DI GCAATG 2 cut(s) 405, 558
BseAI TCCGGA 1 cut(s) 231
BseBI CCWGG 2 cut(s) 149, 348
BseDI CCNNGG 2 cut(s) 147, 148
BseGI GGATG 2 cut(s) 240, 390
BseLI CCNNNNNNNGG 1 cut(s) 238
BseMI GCAATG 2 cut(s) 405, 558
BseRI GAGGAG 2 cut(s) 71, 74
BseXI GCAGC 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 276
Bsh1285I CGRYCG 1 cut(s) 271
BshNI GGYRCC 1 cut(s) 182
BsiEI CGRYCG 1 cut(s) 271
BsiSI CCGG 3 cut(s) 186, 232, 336
BslI CCNNNNNNNGG 1 cut(s) 238
Bsp13I TCCGGA 1 cut(s) 231
Bsp143I GATC 1 cut(s) 268
BspEI TCCGGA 1 cut(s) 231
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 184
BspT107I GGYRCC 1 cut(s) 182
BsrDI GCAATG 2 cut(s) 405, 558
BsrFI RCCGGY 1 cut(s) 335
BssAI RCCGGY 1 cut(s) 335
BssECI CCNNGG 2 cut(s) 147, 148
BssMI GATC 1 cut(s) 268
Bst2UI CCWGG 2 cut(s) 149, 348
Bst4CI ACNGT 1 cut(s) 133
Bst6I CTCTTC 2 cut(s) 48, 372
BstC8I GCNNGC 4 cut(s) 295, 333, 388, 565
BstF5I GGATG 2 cut(s) 240, 390
BstFNI CGCG 1 cut(s) 276
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 1 cut(s) 271
BstMBI GATC 1 cut(s) 268
BstMCI CGRYCG 1 cut(s) 271
BstMWI GCNNNNNNNGC 4 cut(s) 87, 90, 219, 303
BstNI CCWGG 2 cut(s) 149, 348
BstNSI RCATGY 1 cut(s) 121
BstSCI CCNGG 2 cut(s) 147, 346
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 419
BstXI CCANNNNNNTGG 1 cut(s) 179
BtsCI GGATG 2 cut(s) 240, 390
Cac8I GCNNGC 4 cut(s) 295, 333, 388, 565
CfoI GCGC 1 cut(s) 432
Cfr10I RCCGGY 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 344
CseI GACGC 1 cut(s) 265
Csp6I GTAC 2 cut(s) 487, 495
CviAII CATG 2 cut(s) 118, 325
CviJI RGCY 6 cut(s) 73, 177, 250, 293, 335, 351
CviKI_1 RGCY 6 cut(s) 73, 177, 250, 293, 335, 351
CviQI GTAC 2 cut(s) 487, 495
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
Eam1104I CTCTTC 2 cut(s) 48, 372
EarI CTCTTC 2 cut(s) 48, 372
EciI GGCGGA 3 cut(s) 65, 108, 148
Eco47I GGWCC 1 cut(s) 344
Eco47III AGCGCT 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 147, 346
FaeI CATG 2 cut(s) 121, 328
FaiI YATR 6 cut(s) 119, 309, 326, 440, 442, 521
FalI AAGNNNNNCTT 2 cut(s) 419, 451
FatI CATG 2 cut(s) 117, 324
Fnu4HI GCNGC 8 cut(s) 82, 85, 88, 91, 162, 223, 274, 433
FokI GGATG 2 cut(s) 247, 377
Fsp4HI GCNGC 8 cut(s) 82, 85, 88, 91, 162, 223, 274, 433
FspBI CTAG 2 cut(s) 470, 568
GlaI GCGC 1 cut(s) 431
GluI GCNGC 8 cut(s) 82, 85, 88, 91, 162, 223, 274, 433
HaeII RGCGCY 1 cut(s) 433
HapII CCGG 3 cut(s) 186, 232, 336
HgaI GACGC 1 cut(s) 265
HhaI GCGC 1 cut(s) 432
Hin1II CATG 2 cut(s) 121, 328
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HpaII CCGG 3 cut(s) 186, 232, 336
HphI GGTGA 3 cut(s) 257, 412, 451
Hpy166II GTNNAC 1 cut(s) 497
Hpy188III TCNNGA 3 cut(s) 172, 232, 254
Hpy8I GTNNAC 1 cut(s) 497
Hpy99I CGWCG 3 cut(s) 137, 269, 281
HpyAV CCTTC 1 cut(s) 404
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 476
HpyCH4V TGCA 1 cut(s) 405
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 90, 219, 303
HpySE526I ACGT 1 cut(s) 476
Hsp92II CATG 2 cut(s) 121, 328
HspAI GCGC 1 cut(s) 430
Kpn2I TCCGGA 1 cut(s) 231
Kzo9I GATC 1 cut(s) 268
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 1 cut(s) 419
LweI GCATC 1 cut(s) 399
MaeI CTAG 2 cut(s) 470, 568
MaeII ACGT 1 cut(s) 476
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MboII GAAGA 8 cut(s) 65, 203, 206, 209, 212, 215, 331, 359
MluCI AATT 1 cut(s) 512
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 6 cut(s) 18, 40, 49, 52, 63, 235
MroI TCCGGA 1 cut(s) 231
MseI TTAA 1 cut(s) 516
MspI CCGG 3 cut(s) 186, 232, 336
MspR9I CCNGG 2 cut(s) 149, 348
MvaI CCWGG 2 cut(s) 149, 348
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 4 cut(s) 87, 90, 219, 303
NdeII GATC 1 cut(s) 268
NlaIII CATG 2 cut(s) 121, 328
NlaIV GGNNCC 1 cut(s) 184
NspI RCATGY 1 cut(s) 121
PasI CCCWGGG 1 cut(s) 148
PcsI WCGNNNNNNNCGW 2 cut(s) 261, 273
PkrI GCNGC 8 cut(s) 83, 86, 89, 92, 163, 224, 275, 434
Ple19I CGATCG 1 cut(s) 271
Psp6I CCWGG 2 cut(s) 147, 346
PspGI CCWGG 2 cut(s) 147, 346
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 1 cut(s) 344
PvuI CGATCG 1 cut(s) 271
RsaI GTAC 2 cut(s) 488, 496
RsaNI GTAC 2 cut(s) 487, 495
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 8 cut(s) 82, 85, 88, 91, 162, 223, 274, 433
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 1 cut(s) 344
ScrFI CCNGG 2 cut(s) 149, 348
SetI ASST 3 cut(s) 179, 252, 479
SfaNI GCATC 1 cut(s) 399
SinI GGWCC 1 cut(s) 344
Sse9I AATT 1 cut(s) 512
SspMI CTAG 2 cut(s) 470, 568
StyD4I CCNGG 2 cut(s) 147, 346
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 479
TaqI TCGA 3 cut(s) 114, 255, 264
TasI AATT 1 cut(s) 512
TauI GCSGC 7 cut(s) 84, 87, 90, 93, 164, 225, 276
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TseI GCWGC 1 cut(s) 432
TspDTI ATGAA 2 cut(s) 341, 359
TspGWI ACGGA 2 cut(s) 291, 482
VpaK11BI GGWCC 1 cut(s) 344
XapI RAATTY 1 cut(s) 512
XceI RCATGY 1 cut(s) 121
XspI CTAG 2 cut(s) 470, 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.