pycom05g30220

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008620
Physical Location & Seq
Reverse (-)
30394617 .. 30396386
1770 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30220.1

Sequence Viewer

Length: 747 bp
ATGGGGGATTTGGAGAAGCAAGAGACGGTGCTGCTGAAGAAGGCGGAGGAAACGGCGGAAGGGGAAGAAAATGAGAGGCTTTTGGAGGGAAGTGGAATGGCGGTCGTGGATTTCGACCTGCTCTGCTCGACGGTGGCGTTGCAGACCCAGGGCAAGCTGGCGGCCAAGCTCCAGAGCTTCCCCAGAGGAGAGGAAGACGGAGACGCAGGGGACTTGGGTGGCGTCTTCAGGATGTGGGAAGGTGAAGTCCTCGATTGCTTCGATGACCGTCGTGTTGCTCTCGAATCCCTCTGTTGCCCGTGCTATAGATTTGGGAAGAACATGAGACGAGCTGGTTTGGGTCCTTGTTCGTTACAGGGAGCTCTTCATTTGATCCTCGTTGTTCTTGTCCTTCTCAACGGCATTGCCTTCATTGTCACCAAGAAGCACTGCTTTCTATATTTGGCAGTTGCTTTCACCATTTCACTAGGAACGTATTTGGGGTACTTCCGAACACAGATAAAGAAGAAATTTAACATCAGGGGTAGTGATAATTCCTTGGATGATTGCATCTACCATCTCATCTGCCCGTGCTGTGCATTATGTCAGGAATCCAGAACAATGGAGATGAACAATGTCCAAGATGGCACCTGGCATGGTCGGGGTGATACGATATGCATAGGTAGCCTTGGAGAAGGCGCAAAATCGTTCTTTGAGCTACAACCGCCGCCTATTGTCTCGATCAAATCCCCTGACCCCTGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

249

Amino Acids

27.25

Weight (kDa)

5.11

Isoelectric Point (pI)

29.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 79 - 199 6.3e-22 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 126
AccB1I GGYRCC 1 cut(s) 626
AciI CCGC 6 cut(s) 44, 56, 101, 161, 704, 707
AclWI GGATC 1 cut(s) 367
AcoI YGGCCR 1 cut(s) 162
AcsI RAATTY 1 cut(s) 509
AcuI CTGAAG 2 cut(s) 56, 211
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 485
AjnI CCWGG 2 cut(s) 147, 629
AluBI AGCT 7 cut(s) 157, 169, 177, 332, 362, 697, 744
AluI AGCT 7 cut(s) 157, 169, 177, 332, 362, 697, 744
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 4 cut(s) 17, 195, 319, 721
AlwI GGATC 1 cut(s) 367
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 31, 741
ApoI RAATTY 1 cut(s) 509
AspLEI GCGC 1 cut(s) 680
AspS9I GGNCC 1 cut(s) 341
AsuHPI GGTGA 4 cut(s) 254, 409, 448, 656
AvaII GGWCC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 626
BanII GRGCYC 1 cut(s) 364
BbsI GAAGAC 2 cut(s) 201, 217
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 1 cut(s) 18
BccI CCATC 2 cut(s) 564, 617
BceAI ACGGC 2 cut(s) 69, 415
BciT130I CCWGG 2 cut(s) 149, 631
BcoDI GTCTC 4 cut(s) 17, 195, 319, 721
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 2 cut(s) 304, 739
BfuAI ACCTGC 1 cut(s) 126
BisI GCNGC 4 cut(s) 32, 162, 707, 742
BlsI GCNGC 4 cut(s) 33, 163, 708, 743
Bme1390I CCNGG 2 cut(s) 149, 631
Bme18I GGWCC 1 cut(s) 341
BmgT120I GGNCC 1 cut(s) 341
BmiI GGNNCC 2 cut(s) 342, 628
BmrFI CCNGG 2 cut(s) 149, 631
BmsI GCATC 1 cut(s) 558
BpiI GAAGAC 2 cut(s) 201, 217
BpmI CTGGAG 1 cut(s) 155
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 4 cut(s) 147, 148, 537, 667
Bse3DI GCAATG 1 cut(s) 402
BseBI CCWGG 2 cut(s) 149, 631
BseDI CCNNGG 4 cut(s) 147, 148, 537, 667
BseGI GGATG 2 cut(s) 237, 547
BseMI GCAATG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 201
BseXI GCAGC 1 cut(s) 18
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 1 cut(s) 164
BshNI GGYRCC 1 cut(s) 626
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 364
BslFI GGGAC 1 cut(s) 224
BsmAI GTCTC 4 cut(s) 17, 195, 319, 721
BsmBI CGTCTC 3 cut(s) 17, 195, 319
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 1 cut(s) 164
Bsp1286I GDGCHC 1 cut(s) 364
Bsp143I GATC 2 cut(s) 372, 720
BspACI CCGC 6 cut(s) 44, 56, 101, 161, 704, 707
BspANI GGCC 1 cut(s) 164
BspLI GGNNCC 2 cut(s) 342, 628
BspMAI CTGCAG 1 cut(s) 743
BspMI ACCTGC 1 cut(s) 126
BspPI GGATC 1 cut(s) 367
BspQI GCTCTTC 1 cut(s) 369
BspT107I GGYRCC 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 402
BssECI CCNNGG 4 cut(s) 147, 148, 537, 667
BssMI GATC 2 cut(s) 372, 720
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 2 cut(s) 537, 667
Bst2UI CCWGG 2 cut(s) 149, 631
Bst4CI ACNGT 3 cut(s) 28, 133, 269
Bst6I CTCTTC 1 cut(s) 369
BstACI GRCGYC 1 cut(s) 222
BstC8I GCNNGC 2 cut(s) 155, 159
BstF5I GGATG 2 cut(s) 237, 547
BstHHI GCGC 1 cut(s) 680
BstKTI GATC 2 cut(s) 375, 723
BstMAI GTCTC 4 cut(s) 17, 195, 319, 721
BstMBI GATC 2 cut(s) 372, 720
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 2 cut(s) 663, 703
BstNI CCWGG 2 cut(s) 149, 631
BstSCI CCNGG 2 cut(s) 147, 629
BstSFI CTRYAG 2 cut(s) 304, 739
BstV1I GCAGC 1 cut(s) 18
BstV2I GAAGAC 2 cut(s) 201, 217
BstXI CCANNNNNNTGG 1 cut(s) 601
BsuRI GGCC 1 cut(s) 164
BtsCI GGATG 2 cut(s) 237, 547
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 1 cut(s) 427
BveI ACCTGC 1 cut(s) 126
Cac8I GCNNGC 2 cut(s) 155, 159
CfoI GCGC 1 cut(s) 680
Cfr13I GGNCC 1 cut(s) 341
CseI GACGC 2 cut(s) 211, 212
Csp6I GTAC 1 cut(s) 484
CviAII CATG 2 cut(s) 322, 635
CviQI GTAC 1 cut(s) 484
DpnI GATC 2 cut(s) 374, 722
DpnII GATC 2 cut(s) 372, 720
EaeI YGGCCR 1 cut(s) 162
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
EciI GGCGGA 2 cut(s) 59, 71
Ecl136II GAGCTC 1 cut(s) 362
Eco130I CCWWGG 2 cut(s) 537, 667
Eco24I GRGCYC 1 cut(s) 364
Eco47I GGWCC 1 cut(s) 341
Eco53kI GAGCTC 1 cut(s) 362
Eco57I CTGAAG 2 cut(s) 56, 211
EcoICRI GAGCTC 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 147, 629
EcoT14I CCWWGG 2 cut(s) 537, 667
EcoT22I ATGCAT 1 cut(s) 659
EcoT38I GRGCYC 1 cut(s) 364
ErhI CCWWGG 2 cut(s) 537, 667
Esp3I CGTCTC 3 cut(s) 17, 195, 319
FaeI CATG 2 cut(s) 325, 638
FaiI YATR 7 cut(s) 306, 323, 439, 583, 636, 655, 659
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 1 cut(s) 224
FatI CATG 2 cut(s) 321, 634
Fnu4HI GCNGC 4 cut(s) 32, 162, 707, 742
FokI GGATG 2 cut(s) 244, 554
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 4 cut(s) 32, 162, 707, 742
FspBI CTAG 1 cut(s) 467
GlaI GCGC 1 cut(s) 679
GluI GCNGC 4 cut(s) 32, 162, 707, 742
GsuI CTGGAG 1 cut(s) 155
HaeIII GGCC 1 cut(s) 164
HgaI GACGC 2 cut(s) 211, 212
HhaI GCGC 1 cut(s) 680
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 2 cut(s) 325, 638
Hin6I GCGC 1 cut(s) 678
HinP1I GCGC 1 cut(s) 678
HinfI GANTC 2 cut(s) 284, 590
HphI GGTGA 4 cut(s) 254, 409, 448, 656
Hpy188I TCNGA 1 cut(s) 491
Hpy188III TCNNGA 6 cut(s) 172, 229, 281, 587, 594, 718
Hpy99I CGWCG 2 cut(s) 133, 273
HpyAV CCTTC 6 cut(s) 34, 53, 233, 401, 418, 668
HpyCH4III ACNGT 3 cut(s) 28, 133, 269
HpyCH4IV ACGT 1 cut(s) 473
HpyCH4V TGCA 5 cut(s) 142, 549, 578, 657, 741
HpyF10VI GCNNNNNNNGC 2 cut(s) 663, 703
HpySE526I ACGT 1 cut(s) 473
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 2 cut(s) 325, 638
HspAI GCGC 1 cut(s) 678
Kzo9I GATC 2 cut(s) 372, 720
LguI GCTCTTC 1 cut(s) 369
LmnI GCTCC 2 cut(s) 174, 359
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 1 cut(s) 558
MaeI CTAG 1 cut(s) 467
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 2 cut(s) 351, 415
MalI GATC 2 cut(s) 374, 722
MboI GATC 2 cut(s) 372, 720
MboII GAAGA 7 cut(s) 49, 77, 206, 217, 328, 356, 517
MhlI GDGCHC 1 cut(s) 364
MluCI AATT 2 cut(s) 509, 532
MnlI CCTC 8 cut(s) 40, 69, 79, 179, 184, 260, 299, 386
Mph1103I ATGCAT 1 cut(s) 659
MseI TTAA 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 744
MspR9I CCNGG 2 cut(s) 149, 631
MvaI CCWGG 2 cut(s) 149, 631
MwoI GCNNNNNNNGC 2 cut(s) 663, 703
NdeII GATC 2 cut(s) 372, 720
NlaIII CATG 2 cut(s) 325, 638
NlaIV GGNNCC 2 cut(s) 342, 628
NmuCI GTSAC 1 cut(s) 415
NsiI ATGCAT 1 cut(s) 659
PasI CCCWGGG 1 cut(s) 148
PciSI GCTCTTC 1 cut(s) 369
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 134, 258
PfeI GAWTC 2 cut(s) 284, 590
PkrI GCNGC 4 cut(s) 33, 163, 708, 743
PpuMI RGGWCCY 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 341
Psp6I CCWGG 2 cut(s) 147, 629
PspGI CCWGG 2 cut(s) 147, 629
PspN4I GGNNCC 2 cut(s) 342, 628
PspPI GGNCC 1 cut(s) 341
PspPPI RGGWCCY 1 cut(s) 341
PstI CTGCAG 1 cut(s) 743
PvuII CAGCTG 1 cut(s) 744
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
SacI GAGCTC 1 cut(s) 364
SapI GCTCTTC 1 cut(s) 369
SaqAI TTAA 1 cut(s) 513
SatI GCNGC 4 cut(s) 32, 162, 707, 742
Sau3AI GATC 2 cut(s) 372, 720
Sau96I GGNCC 1 cut(s) 341
ScrFI CCNGG 2 cut(s) 149, 631
SduI GDGCHC 1 cut(s) 364
SfaNI GCATC 1 cut(s) 558
SfcI CTRYAG 2 cut(s) 304, 739
SinI GGWCC 1 cut(s) 341
Sse9I AATT 2 cut(s) 509, 532
SsiI CCGC 6 cut(s) 44, 56, 101, 161, 704, 707
SspMI CTAG 1 cut(s) 467
SstI GAGCTC 1 cut(s) 364
StyD4I CCNGG 2 cut(s) 147, 629
StyI CCWWGG 2 cut(s) 537, 667
TaaI ACNGT 3 cut(s) 28, 133, 269
TaiI ACGT 1 cut(s) 476
TaqI TCGA 6 cut(s) 114, 128, 252, 261, 282, 719
TasI AATT 2 cut(s) 509, 532
TauI GCSGC 2 cut(s) 164, 709
TfiI GAWTC 2 cut(s) 284, 590
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TscAI CASTG 1 cut(s) 434
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 2 cut(s) 31, 741
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 3 cut(s) 356, 400, 623
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 1 cut(s) 434
VpaK11BI GGWCC 1 cut(s) 341
XapI RAATTY 1 cut(s) 509
XspI CTAG 1 cut(s) 467
Zsp2I ATGCAT 1 cut(s) 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.