FvH4_3g06420

Ribosomal_L32e

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003194
Physical Location & Seq
Forward (+)
3690738 .. 3692554
1817 bp
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UTR
Exon/CDS
Intron
FvH4_3g06420.t2

Sequence Viewer

Length: 402 bp
ATGGCGGTTCCGTTGTTGAAGAAGTCGATCGTGAAGAAGAGGGTCAAGCAGTTCAAGCGGCCCCAGAGCGACCGGAAGATCTGCGTCAAGACCAACTGGAGGAGGCCTAAGGGTATTGACTCTCGTGTACGAAGAAAGTTCAAGGGAGTTACTCTCATGCCCAACATTGGTTACGGTTCTGACAAGAAGACTCGCCATTACCTACCCAATCGTTTCAAGAAGTTTGTTGTCCACAATGTCAAGGAGCTTGAAGTTCTGATGATGCACAATATGACCTATTGTGCTGAAATTGCACACAATATCTCCACAAGGAAGAGGAAGGATATTGTTGAGAGAGCTGCCCAGCTTGATGTGGTTGTCACTAACAAGCTTGCTAGGTTGCGAAGCCAAGAGGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L32e

Protein Analysis

134

Amino Acids

15.72

Weight (kDa)

10.7

Isoelectric Point (pI)

60.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L32e PF01655 16 - 122 8.6e-47 Ribosomal protein L32
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 58
AfaI GTAC 1 cut(s) 129
AfiI CCNNNNNNNGG 2 cut(s) 99, 167
AgsI TTSAA 5 cut(s) 19, 55, 142, 217, 251
AluBI AGCT 4 cut(s) 247, 338, 346, 370
AluI AGCT 4 cut(s) 247, 338, 346, 370
AoxI GGCC 2 cut(s) 59, 104
ApeKI GCWGC 1 cut(s) 338
AspS9I GGNCC 1 cut(s) 60
AxyI CCTNAGG 1 cut(s) 108
BauI CACGAG 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 194
BbvI GCAGC 1 cut(s) 325
BfaI CTAG 1 cut(s) 375
BglII AGATCT 1 cut(s) 78
BisI GCNGC 2 cut(s) 59, 339
BlsI GCNGC 2 cut(s) 60, 340
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 2 cut(s) 9, 62
BmsI GCATC 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 194
BplI GAGNNNNNCTC 2 cut(s) 138, 170
BpmI CTGGAG 1 cut(s) 118
BsaWI WCCGGW 1 cut(s) 72
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 167
Bse1I ACTGG 1 cut(s) 101
Bse21I CCTNAGG 1 cut(s) 108
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 2 cut(s) 99, 167
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 1 cut(s) 115
BseXI GCAGC 1 cut(s) 325
BseYI CCCAGC 1 cut(s) 342
Bsh1285I CGRYCG 2 cut(s) 30, 73
BshFI GGCC 2 cut(s) 61, 106
BsiEI CGRYCG 2 cut(s) 30, 73
BsiSI CCGG 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 99, 167
BsnI GGCC 2 cut(s) 61, 106
Bsp143I GATC 2 cut(s) 27, 78
BspACI CCGC 2 cut(s) 5, 58
BspANI GGCC 2 cut(s) 61, 106
BspLI GGNNCC 2 cut(s) 9, 62
BsrI ACTGG 1 cut(s) 101
BssMI GATC 2 cut(s) 27, 78
BssSI CACGAG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 2 cut(s) 32, 308
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 400
BstKTI GATC 2 cut(s) 30, 81
BstMBI GATC 2 cut(s) 27, 78
BstMCI CGRYCG 2 cut(s) 30, 73
BstMWI GCNNNNNNNGC 2 cut(s) 55, 290
BstV1I GCAGC 1 cut(s) 325
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
Bsu36I CCTNAGG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 61, 106
BtsCI GGATG 1 cut(s) 400
Cac8I GCNNGC 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 60
CseI GACGC 1 cut(s) 73
Csp6I GTAC 1 cut(s) 128
CviAII CATG 1 cut(s) 157
CviJI RGCY 7 cut(s) 61, 106, 247, 338, 346, 370, 387
CviKI_1 RGCY 7 cut(s) 61, 106, 247, 338, 346, 370, 387
CviQI GTAC 1 cut(s) 128
DdeI CTNAG 1 cut(s) 108
DpnI GATC 2 cut(s) 29, 80
DpnII GATC 2 cut(s) 27, 78
Eam1104I CTCTTC 2 cut(s) 32, 308
EarI CTCTTC 2 cut(s) 32, 308
Eco147I AGGCCT 1 cut(s) 106
Eco81I CCTNAGG 1 cut(s) 108
FaeI CATG 1 cut(s) 160
FaiI YATR 2 cut(s) 158, 272
FatI CATG 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 59, 339
Fsp4HI GCNGC 2 cut(s) 59, 339
FspBI CTAG 1 cut(s) 375
GluI GCNGC 2 cut(s) 59, 339
GsaI CCCAGC 1 cut(s) 346
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 2 cut(s) 61, 106
HapII CCGG 1 cut(s) 73
HgaI GACGC 1 cut(s) 73
Hin1II CATG 1 cut(s) 160
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 2 cut(s) 119, 190
HpaII CCGG 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 128, 232
Hpy188I TCNGA 2 cut(s) 181, 258
Hpy188III TCNNGA 3 cut(s) 31, 88, 217
Hpy8I GTNNAC 2 cut(s) 128, 232
HpyAV CCTTC 1 cut(s) 313
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4V TGCA 2 cut(s) 265, 293
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 290
HpyF3I CTNAG 1 cut(s) 108
Hsp92II CATG 1 cut(s) 160
Kzo9I GATC 2 cut(s) 27, 78
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 4 cut(s) 77, 82, 86, 356
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 1 cut(s) 252
MaeI CTAG 1 cut(s) 375
MaeIII GTNAC 3 cut(s) 148, 170, 358
MalI GATC 2 cut(s) 29, 80
MboI GATC 2 cut(s) 27, 78
MboII GAAGA 7 cut(s) 31, 46, 49, 88, 144, 199, 325
MflI RGATCY 1 cut(s) 78
MluCI AATT 1 cut(s) 288
MlyI GAGTC 2 cut(s) 113, 184
MnlI CCTC 5 cut(s) 33, 93, 96, 309, 385
MspI CCGG 1 cut(s) 73
MwoI GCNNNNNNNGC 2 cut(s) 55, 290
NdeII GATC 2 cut(s) 27, 78
NlaIII CATG 1 cut(s) 160
NlaIV GGNNCC 2 cut(s) 9, 62
NmuCI GTSAC 1 cut(s) 358
PceI AGGCCT 1 cut(s) 106
PkrI GCNGC 2 cut(s) 60, 340
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 2 cut(s) 113, 184
PpsI GAGTC 2 cut(s) 113, 184
PspFI CCCAGC 1 cut(s) 342
PspN4I GGNNCC 2 cut(s) 9, 62
PspPI GGNCC 1 cut(s) 60
PsuI RGATCY 1 cut(s) 78
PvuI CGATCG 1 cut(s) 30
RsaI GTAC 1 cut(s) 129
RsaNI GTAC 1 cut(s) 128
SatI GCNGC 2 cut(s) 59, 339
Sau3AI GATC 2 cut(s) 27, 78
Sau96I GGNCC 1 cut(s) 60
SchI GAGTC 2 cut(s) 113, 184
SetI ASST 7 cut(s) 204, 249, 278, 340, 348, 372, 380
SfaNI GCATC 1 cut(s) 252
Sse9I AATT 1 cut(s) 288
SseBI AGGCCT 1 cut(s) 106
SsiI CCGC 2 cut(s) 5, 58
SspMI CTAG 1 cut(s) 375
StuI AGGCCT 1 cut(s) 106
TaaI ACNGT 1 cut(s) 176
TaqI TCGA 1 cut(s) 26
TasI AATT 1 cut(s) 288
TauI GCSGC 1 cut(s) 61
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 358
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.