FvH4_6g42210

RIBOSOMAL protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003194
Physical Location & Seq
Forward (+)
32987998 .. 32989081
1084 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42210.t1

Sequence Viewer

Length: 237 bp
ATGGCGGTTCCGTTGTTGAAGAAGGCGATCGTGAAGAAGAGGGTCAAGCAGTTCAAGCGGCCCCAGAGCGATCGCAAGATACGGTCAACTGGTCAAGATGAAATTTCAAGGGAGTTTACTCTCATGCGCAACATTGGTTATGGTTCTGACAAGAAGACTCGCCATTACCTTCCCAATCATTTCAAGAAGTTTGTTGTCCACGACGTGAAGATGCTTGGAAGTTCTGATGATGCATAA

Protein Analysis

79

Amino Acids

9.1

Weight (kDa)

10.37

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L32e PF01655 33 - 72 2.7e-08 Ribosomal protein L32
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 128
AciI CCGC 2 cut(s) 5, 58
AcsI RAATTY 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 205
AgsI TTSAA 4 cut(s) 19, 55, 108, 184
AjiI CACGTC 1 cut(s) 205
AoxI GGCC 1 cut(s) 59
ApoI RAATTY 1 cut(s) 102
AsiSI GCGATCGC 1 cut(s) 73
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 60
BbsI GAAGAC 1 cut(s) 161
BisI GCNGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 60
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 2 cut(s) 9, 62
BmsI GCATC 2 cut(s) 201, 220
BpiI GAAGAC 1 cut(s) 161
Bse1I ACTGG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 94
Bsh1285I CGRYCG 2 cut(s) 30, 73
BshFI GGCC 1 cut(s) 61
BsiEI CGRYCG 2 cut(s) 30, 73
BsnI GGCC 1 cut(s) 61
Bsp143I GATC 2 cut(s) 27, 70
BspACI CCGC 2 cut(s) 5, 58
BspANI GGCC 1 cut(s) 61
BspLI GGNNCC 2 cut(s) 9, 62
BsrI ACTGG 1 cut(s) 94
BssMI GATC 2 cut(s) 27, 70
Bst4CI ACNGT 1 cut(s) 84
Bst6I CTCTTC 1 cut(s) 32
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 2 cut(s) 30, 73
BstMBI GATC 2 cut(s) 27, 70
BstMCI CGRYCG 2 cut(s) 30, 73
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 161
BsuRI GGCC 1 cut(s) 61
BtrI CACGTC 1 cut(s) 205
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 1 cut(s) 60
CviAII CATG 1 cut(s) 124
CviJI RGCY 1 cut(s) 61
CviKI_1 RGCY 1 cut(s) 61
DpnI GATC 2 cut(s) 29, 72
DpnII GATC 2 cut(s) 27, 70
DraIII CACNNNGTG 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EcoT22I ATGCAT 1 cut(s) 235
FaeI CATG 1 cut(s) 127
FaiI YATR 3 cut(s) 125, 141, 235
FatI CATG 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 59
Fsp4HI GCNGC 1 cut(s) 59
FspI TGCGCA 1 cut(s) 128
GlaI GCGC 1 cut(s) 128
GluI GCNGC 1 cut(s) 59
HaeIII GGCC 1 cut(s) 61
HhaI GCGC 1 cut(s) 129
Hin1II CATG 1 cut(s) 127
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 1 cut(s) 157
Hpy166II GTNNAC 3 cut(s) 87, 117, 199
Hpy188I TCNGA 2 cut(s) 148, 226
Hpy188III TCNNGA 3 cut(s) 31, 95, 184
Hpy8I GTNNAC 3 cut(s) 87, 117, 199
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 2 cut(s) 16, 179
HpyCH4III ACNGT 1 cut(s) 84
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 1 cut(s) 233
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 1 cut(s) 127
HspAI GCGC 1 cut(s) 127
Kzo9I GATC 2 cut(s) 27, 70
LpnPI CCDG 2 cut(s) 75, 77
LweI GCATC 2 cut(s) 201, 220
MaeII ACGT 1 cut(s) 204
MalI GATC 2 cut(s) 29, 72
MboI GATC 2 cut(s) 27, 70
MboII GAAGA 5 cut(s) 31, 46, 49, 166, 220
MluCI AATT 1 cut(s) 102
MlyI GAGTC 1 cut(s) 151
MnlI CCTC 1 cut(s) 33
Mph1103I ATGCAT 1 cut(s) 235
MwoI GCNNNNNNNGC 1 cut(s) 55
NdeII GATC 2 cut(s) 27, 70
NlaIII CATG 1 cut(s) 127
NlaIV GGNNCC 2 cut(s) 9, 62
NsbI TGCGCA 1 cut(s) 128
NsiI ATGCAT 1 cut(s) 235
PkrI GCNGC 1 cut(s) 60
Ple19I CGATCG 2 cut(s) 30, 73
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
PspN4I GGNNCC 2 cut(s) 9, 62
PspPI GGNCC 1 cut(s) 60
PvuI CGATCG 2 cut(s) 30, 73
RgaI GCGATCGC 1 cut(s) 73
SatI GCNGC 1 cut(s) 59
Sau3AI GATC 2 cut(s) 27, 70
Sau96I GGNCC 1 cut(s) 60
SchI GAGTC 1 cut(s) 151
SetI ASST 2 cut(s) 171, 207
SfaAI GCGATCGC 1 cut(s) 73
SfaNI GCATC 2 cut(s) 201, 220
SgfI GCGATCGC 1 cut(s) 73
Sse9I AATT 1 cut(s) 102
SsiI CCGC 2 cut(s) 5, 58
TaaI ACNGT 1 cut(s) 84
TaiI ACGT 1 cut(s) 207
TasI AATT 1 cut(s) 102
TauI GCSGC 1 cut(s) 61
TspDTI ATGAA 1 cut(s) 114
XapI RAATTY 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.