MD05G1164800.v1.1

60s ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003194
Physical Location & Seq
Forward (+)
29341039 .. 29342943
1905 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1164800.v1.1.491

Sequence Viewer

Length: 318 bp
ATGAGTGAAAGCTGGAGAAGGCCCAAGGGTATTGATTCACGTGTCAGGCGAAAGTTCAAGGGATGTGCGTTGATGCCCAACATTGGCTATGGGTCAGACAAGAAGACTCGTCACTATCTGCCTAACAAGTTCAAGAAATTTGTTGTGCACAATGTGAAAGAGGTTGAACTTCTAATGATGCACAACAGGACCTACTGTGCTGAGATAGCACACAACATTTCCACCAGGAAGAGAAAGGAGATCGTCGAGCGTGCAGCCCAGTTGGATGTTGTTGTTACCAACAAACTTGCCAGGTTGCGCAGCCAGGAGGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0001889 GO:0002181 GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005783 GO:0005789 GO:0005791 GO:0005829 GO:0005840 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006412 GO:0006413 GO:0006518 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009410 GO:0009719 GO:0009725 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0012505 GO:0014070 GO:0015031 GO:0015833 GO:0015934 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0019538 GO:0022625 GO:0022626 GO:0030867 GO:0031090 GO:0031099 GO:0031100 GO:0031960 GO:0031974 GO:0031981 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033036 GO:0033365 GO:0033993 GO:0034613 GO:0034641 GO:0034645 GO:0034655 GO:0035690 GO:0042175 GO:0042221 GO:0042493 GO:0042788 GO:0042886 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048513 GO:0048519 GO:0048545 GO:0048731 GO:0048732 GO:0048856 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0051384 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061008 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0070972 GO:0071310 GO:0071383 GO:0071384 GO:0071385 GO:0071396 GO:0071407 GO:0071466 GO:0071495 GO:0071548 GO:0071549 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0072657 GO:0090150 GO:0090304 GO:0097327 GO:0097421 GO:0098552 GO:0098554 GO:0098556 GO:0098562 GO:0098588 GO:0098827 GO:1901360 GO:1901361 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901654 GO:1901655 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03010 ko03022 ko04080 ko04141 ko04725 map03010 map03022 map04080 map04141 map04725
Pfam Domains
Protein Families
Ribosomal_L32e

Protein Analysis

106

Amino Acids

12.38

Weight (kDa)

10.17

Isoelectric Point (pI)

76.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L32e PF01655 2 - 94 2.6e-42 Ribosomal protein L32
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 299
AcsI RAATTY 1 cut(s) 137
AcvI CACGTG 1 cut(s) 41
AdeI CACNNNGTG 1 cut(s) 154
AfiI CCNNNNNNNGG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 40
AgsI TTSAA 3 cut(s) 58, 133, 167
AjnI CCWGG 3 cut(s) 224, 290, 303
AluBI AGCT 1 cut(s) 12
AluI AGCT 1 cut(s) 12
Alw21I GWGCWC 1 cut(s) 150
Alw44I GTGCAC 1 cut(s) 146
AoxI GGCC 1 cut(s) 20
ApaLI GTGCAC 1 cut(s) 146
ApeKI GCWGC 2 cut(s) 254, 300
ApoI RAATTY 1 cut(s) 137
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 2 cut(s) 21, 189
AvaII GGWCC 1 cut(s) 189
BaeGI GKGCMC 1 cut(s) 150
BbrPI CACGTG 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 2 cut(s) 266, 312
BciT130I CCWGG 3 cut(s) 226, 292, 305
BisI GCNGC 2 cut(s) 255, 301
BlsI GCNGC 2 cut(s) 256, 302
Bme1390I CCNGG 3 cut(s) 226, 292, 305
Bme18I GGWCC 1 cut(s) 189
BmgT120I GGNCC 2 cut(s) 21, 189
BmrFI CCNGG 3 cut(s) 226, 292, 305
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 2 cut(s) 63, 168
BmuI ACTGGG 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 110
BpmI CTGGAG 1 cut(s) 34
BsaAI YACGTR 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 24
BsaXI ACNNNNNCTCC 1 cut(s) 299
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 259
BseBI CCWGG 3 cut(s) 226, 292, 305
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 3 cut(s) 68, 271, 316
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 1 cut(s) 259
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 2 cut(s) 266, 312
BsgI GTGCAG 1 cut(s) 273
BshFI GGCC 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 150
BslI CCNNNNNNNGG 1 cut(s) 83
BsnI GGCC 1 cut(s) 22
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 1 cut(s) 240
BspANI GGCC 1 cut(s) 22
BspCNI CTCAG 1 cut(s) 193
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 24
Bst2UI CCWGG 3 cut(s) 226, 292, 305
Bst4CI ACNGT 1 cut(s) 197
Bst6I CTCTTC 1 cut(s) 224
BstBAI YACGTR 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 1 cut(s) 201
BstF5I GGATG 3 cut(s) 68, 271, 316
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 1 cut(s) 243
BstMBI GATC 1 cut(s) 240
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstNI CCWGG 3 cut(s) 226, 292, 305
BstSCI CCNGG 3 cut(s) 224, 290, 303
BstSLI GKGCMC 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 266, 312
BstV2I GAAGAC 1 cut(s) 110
BsuRI GGCC 1 cut(s) 22
BtsCI GGATG 3 cut(s) 68, 271, 316
Cac8I GCNNGC 1 cut(s) 252
CfoI GCGC 1 cut(s) 300
Cfr13I GGNCC 2 cut(s) 21, 189
CviJI RGCY 5 cut(s) 12, 22, 87, 257, 303
CviKI_1 RGCY 5 cut(s) 12, 22, 87, 257, 303
DdeI CTNAG 1 cut(s) 201
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
DraIII CACNNNGTG 1 cut(s) 154
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Eco130I CCWWGG 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 189
Eco72I CACGTG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 189
EcoRII CCWGG 3 cut(s) 224, 290, 303
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaiI YATR 1 cut(s) 90
Fnu4HI GCNGC 2 cut(s) 255, 301
FokI GGATG 2 cut(s) 75, 278
Fsp4HI GCNGC 2 cut(s) 255, 301
FspI TGCGCA 1 cut(s) 299
GlaI GCGC 1 cut(s) 299
GluI GCNGC 2 cut(s) 255, 301
GsuI CTGGAG 1 cut(s) 34
HaeIII GGCC 1 cut(s) 22
HhaI GCGC 1 cut(s) 300
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HinfI GANTC 2 cut(s) 35, 106
Hpy166II GTNNAC 1 cut(s) 148
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 1 cut(s) 148
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 1 cut(s) 12
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 3 cut(s) 148, 181, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 1 cut(s) 201
HpySE526I ACGT 1 cut(s) 40
HspAI GCGC 1 cut(s) 298
Kzo9I GATC 1 cut(s) 240
LpnPI CCDG 8 cut(s) 31, 172, 211, 238, 272, 277, 290, 304
Lsp1109I GCAGC 2 cut(s) 266, 312
LweI GCATC 2 cut(s) 63, 168
MaeII ACGT 1 cut(s) 40
MaeIII GTNAC 2 cut(s) 110, 274
MalI GATC 1 cut(s) 242
MboI GATC 1 cut(s) 240
MboII GAAGA 2 cut(s) 115, 241
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 1 cut(s) 137
MlyI GAGTC 1 cut(s) 100
MmeI TCCRAC 1 cut(s) 243
MnlI CCTC 2 cut(s) 154, 301
MspR9I CCNGG 3 cut(s) 226, 292, 305
MvaI CCWGG 3 cut(s) 226, 292, 305
MwoI GCNNNNNNNGC 1 cut(s) 206
NdeII GATC 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 110
NsbI TGCGCA 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 2 cut(s) 256, 302
PleI GAGTC 1 cut(s) 100
PmaCI CACGTG 1 cut(s) 41
PmlI CACGTG 1 cut(s) 41
PpsI GAGTC 1 cut(s) 100
Ppu21I YACGTR 1 cut(s) 41
PpuMI RGGWCCY 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 189
Psp6I CCWGG 3 cut(s) 224, 290, 303
PspCI CACGTG 1 cut(s) 41
PspGI CCWGG 3 cut(s) 224, 290, 303
PspPI GGNCC 2 cut(s) 21, 189
PspPPI RGGWCCY 1 cut(s) 189
SatI GCNGC 2 cut(s) 255, 301
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 2 cut(s) 21, 189
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 3 cut(s) 226, 292, 305
SduI GDGCHC 1 cut(s) 150
SetI ASST 5 cut(s) 14, 43, 165, 194, 296
SfaNI GCATC 2 cut(s) 63, 168
SinI GGWCC 1 cut(s) 189
Sse9I AATT 1 cut(s) 137
StyD4I CCNGG 3 cut(s) 224, 290, 303
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 197
TaiI ACGT 1 cut(s) 43
TaqI TCGA 1 cut(s) 246
TasI AATT 1 cut(s) 137
TfiI GAWTC 1 cut(s) 35
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 2 cut(s) 254, 300
Tsp45I GTSAC 1 cut(s) 110
VneI GTGCAC 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 189
XapI RAATTY 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.