MD10G1267800.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003194
Physical Location & Seq
Forward (+)
36076545 .. 36079021
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1267800.v1.1.491

Sequence Viewer

Length: 315 bp
ATGCCCAGCTGGAGAAGACCAAAGGGTATTGATTCTCGTGTTAGAAGAAAGTTCAAGGGCTGTGTTCTCATGCCCAACATTGGTTATGGGTCAGACAAGAAGACCCGTCATTATCTTCCCAATCATTTCAAGAAGTTTGTTGTGCACAACGTCAAGGAGCTCGAACTGCTGATGATGCACAATAGGACCTACTGCGCCGAGATTGCACACAATGTGTCCACAAGGAAGAGGAAAGAAATTGTTGAGAGAGCCGCCCAGCTTGACGTTGTTGTTACGAACAAGTTGGCCAGGTTGCGCAGCCAGGAAGATGAATGA

Protein Analysis

105

Amino Acids

12.3

Weight (kDa)

10.24

Isoelectric Point (pI)

67.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L32e PF01655 3 - 93 7.2e-43 Ribosomal protein L32
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 296
AciI CCGC 1 cut(s) 252
AcoI YGGCCR 1 cut(s) 285
AdeI CACNNNGTG 1 cut(s) 214
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 2 cut(s) 55, 130
AjnI CCWGG 2 cut(s) 287, 300
AluBI AGCT 3 cut(s) 9, 160, 259
AluI AGCT 3 cut(s) 9, 160, 259
Alw21I GWGCWC 2 cut(s) 147, 162
Alw44I GTGCAC 1 cut(s) 143
AoxI GGCC 1 cut(s) 285
ApaLI GTGCAC 1 cut(s) 143
ApeKI GCWGC 1 cut(s) 297
AspLEI GCGC 2 cut(s) 197, 297
AspS9I GGNCC 1 cut(s) 186
AvaII GGWCC 1 cut(s) 186
BaeGI GKGCMC 1 cut(s) 147
BalI TGGCCA 1 cut(s) 287
BanII GRGCYC 1 cut(s) 162
BauI CACGAG 1 cut(s) 36
BbsI GAAGAC 2 cut(s) 22, 107
Bbv12I GWGCWC 2 cut(s) 147, 162
BbvI GCAGC 1 cut(s) 309
BciT130I CCWGG 2 cut(s) 289, 302
BisI GCNGC 2 cut(s) 252, 298
BlsI GCNGC 2 cut(s) 253, 299
Bme1390I CCNGG 2 cut(s) 289, 302
Bme18I GGWCC 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 186
BmrFI CCNGG 2 cut(s) 289, 302
BmsI GCATC 1 cut(s) 165
BpiI GAAGAC 2 cut(s) 22, 107
BpmI CTGGAG 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 80
BseBI CCWGG 2 cut(s) 289, 302
BseLI CCNNNNNNNGG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 147
BseXI GCAGC 1 cut(s) 309
BseYI CCCAGC 2 cut(s) 5, 255
BshFI GGCC 1 cut(s) 287
BsiHKAI GWGCWC 2 cut(s) 147, 162
BslI CCNNNNNNNGG 1 cut(s) 80
BsnI GGCC 1 cut(s) 287
Bsp1286I GDGCHC 2 cut(s) 147, 162
BspACI CCGC 1 cut(s) 252
BspANI GGCC 1 cut(s) 287
BssSI CACGAG 1 cut(s) 36
Bst2BI CACGAG 1 cut(s) 36
Bst2UI CCWGG 2 cut(s) 289, 302
Bst6I CTCTTC 1 cut(s) 221
BstHHI GCGC 2 cut(s) 197, 297
BstMWI GCNNNNNNNGC 3 cut(s) 166, 175, 203
BstNI CCWGG 2 cut(s) 289, 302
BstSCI CCNGG 2 cut(s) 287, 300
BstSLI GKGCMC 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 309
BstV2I GAAGAC 2 cut(s) 22, 107
BsuRI GGCC 1 cut(s) 287
CfoI GCGC 2 cut(s) 197, 297
Cfr13I GGNCC 1 cut(s) 186
CviAII CATG 1 cut(s) 70
CviJI RGCY 7 cut(s) 9, 60, 160, 251, 259, 287, 300
CviKI_1 RGCY 7 cut(s) 9, 60, 160, 251, 259, 287, 300
DraIII CACNNNGTG 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 162
Eco47I GGWCC 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 160
EcoICRI GAGCTC 1 cut(s) 160
EcoO109I RGGNCCY 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 287, 300
EcoT38I GRGCYC 1 cut(s) 162
FaeI CATG 1 cut(s) 73
FaiI YATR 2 cut(s) 71, 87
FatI CATG 1 cut(s) 69
Fnu4HI GCNGC 2 cut(s) 252, 298
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 2 cut(s) 252, 298
FspI TGCGCA 1 cut(s) 296
GlaI GCGC 2 cut(s) 196, 296
GluI GCNGC 2 cut(s) 252, 298
GsaI CCCAGC 2 cut(s) 9, 259
GsuI CTGGAG 1 cut(s) 31
HaeIII GGCC 1 cut(s) 287
HhaI GCGC 2 cut(s) 197, 297
Hin1II CATG 1 cut(s) 73
Hin6I GCGC 2 cut(s) 195, 295
HinP1I GCGC 2 cut(s) 195, 295
HinfI GANTC 1 cut(s) 32
Hpy166II GTNNAC 2 cut(s) 145, 219
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 1 cut(s) 130
Hpy8I GTNNAC 2 cut(s) 145, 219
HpyCH4IV ACGT 2 cut(s) 150, 264
HpyCH4V TGCA 3 cut(s) 145, 178, 206
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 175, 203
HpySE526I ACGT 2 cut(s) 150, 264
Hsp92II CATG 1 cut(s) 73
HspAI GCGC 2 cut(s) 195, 295
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 5 cut(s) 19, 269, 274, 287, 301
Lsp1109I GCAGC 1 cut(s) 309
LweI GCATC 1 cut(s) 165
MaeII ACGT 2 cut(s) 150, 264
MaeIII GTNAC 1 cut(s) 271
MboII GAAGA 5 cut(s) 27, 57, 107, 112, 238
MhlI GDGCHC 2 cut(s) 147, 162
MlsI TGGCCA 1 cut(s) 287
MluCI AATT 1 cut(s) 237
MluNI TGGCCA 1 cut(s) 287
MnlI CCTC 1 cut(s) 222
Mox20I TGGCCA 1 cut(s) 287
MscI TGGCCA 1 cut(s) 287
Msp20I TGGCCA 1 cut(s) 287
MspA1I CMGCKG 1 cut(s) 9
MspR9I CCNGG 2 cut(s) 289, 302
MvaI CCWGG 2 cut(s) 289, 302
MwoI GCNNNNNNNGC 3 cut(s) 166, 175, 203
NlaIII CATG 1 cut(s) 73
NmeAIII GCCGAG 1 cut(s) 223
NsbI TGCGCA 1 cut(s) 296
PfeI GAWTC 1 cut(s) 32
PkrI GCNGC 2 cut(s) 253, 299
PpuMI RGGWCCY 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 186
Psp6I CCWGG 2 cut(s) 287, 300
PspFI CCCAGC 2 cut(s) 5, 255
PspGI CCWGG 2 cut(s) 287, 300
PspPI GGNCC 1 cut(s) 186
PspPPI RGGWCCY 1 cut(s) 186
PvuII CAGCTG 1 cut(s) 9
SacI GAGCTC 1 cut(s) 162
SatI GCNGC 2 cut(s) 252, 298
Sau96I GGNCC 1 cut(s) 186
ScrFI CCNGG 2 cut(s) 289, 302
SduI GDGCHC 2 cut(s) 147, 162
SetI ASST 7 cut(s) 11, 153, 162, 191, 261, 267, 293
SfaNI GCATC 1 cut(s) 165
SinI GGWCC 1 cut(s) 186
Sse9I AATT 1 cut(s) 237
SsiI CCGC 1 cut(s) 252
SstI GAGCTC 1 cut(s) 162
StyD4I CCNGG 2 cut(s) 287, 300
TaiI ACGT 2 cut(s) 153, 267
TaqI TCGA 1 cut(s) 162
TasI AATT 1 cut(s) 237
TauI GCSGC 1 cut(s) 254
TfiI GAWTC 1 cut(s) 32
TseI GCWGC 1 cut(s) 297
VneI GTGCAC 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.