Rh6DG262100

Ribosomal_L32e

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003194
Physical Location & Seq
Forward (+)
45363156 .. 45365215
2060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG262100.1

Sequence Viewer

Length: 414 bp
ATGACAGGAACAATGGCCGTGCCTTTGCTGAAGAAGCAGATTGTGAAGAAGCGCCTCAAGCAGTTCAAGAGGCCCCAGAGTGACAGGAAGATATGTGTCAAGACAAACTGGAGAAGGCCCAAGGGTATTGATTCACGTGTCAGGCGTAAGTTTAAGGGAGTCACTCTGATGCCTAACATTGGTTATGGCTCTGATAAGAAGACTCGCCACTACCTCCCCAACAAATTTAAGAAATTTGTTGTCCACAACGTCAATGAGTTGGAAGTTCTTATGATGCACAACAGGACTTACTGTGCTGAGATTGCTCACAACATTTCCACCCGGAAGAGAAAGGAGATTGTTGAACGTGCAGCTCAGTTGGATGTTGTTGTCACCAACAAACTCGCCAGGTTGCGCAGCCAGGAAGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L32e

Protein Analysis

137

Amino Acids

16.16

Weight (kDa)

10.7

Isoelectric Point (pI)

62.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L32e PF01655 20 - 126 2.9e-47 Ribosomal protein L32
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 395
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 2 cut(s) 224, 233
AcuI CTGAAG 1 cut(s) 50
AcvI CACGTG 1 cut(s) 137
AfiI CCNNNNNNNGG 1 cut(s) 179
AflIII ACRYGT 1 cut(s) 136
AgsI TTSAA 2 cut(s) 67, 344
AjnI CCWGG 2 cut(s) 386, 399
AluBI AGCT 1 cut(s) 353
AluI AGCT 1 cut(s) 353
AoxI GGCC 3 cut(s) 15, 71, 116
ApeKI GCWGC 2 cut(s) 350, 396
ApoI RAATTY 2 cut(s) 224, 233
AspLEI GCGC 2 cut(s) 54, 396
AspS9I GGNCC 2 cut(s) 72, 117
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 364
BbrPI CACGTG 1 cut(s) 137
BbsI GAAGAC 1 cut(s) 206
BbvI GCAGC 2 cut(s) 362, 408
BceAI ACGGC 1 cut(s) 2
BciT130I CCWGG 2 cut(s) 388, 401
BcnI CCSGG 1 cut(s) 322
BfoI RGCGCY 1 cut(s) 55
BisI GCNGC 2 cut(s) 351, 397
BlsI GCNGC 2 cut(s) 352, 398
Bme1390I CCNGG 3 cut(s) 322, 388, 401
BmgT120I GGNCC 2 cut(s) 72, 117
BmiI GGNNCC 1 cut(s) 74
BmrFI CCNGG 3 cut(s) 322, 388, 401
BmsI GCATC 2 cut(s) 159, 264
BpiI GAAGAC 1 cut(s) 206
BpmI CTGGAG 1 cut(s) 130
BpuEI CTTGAG 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 322
BsaAI YACGTR 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 120
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 113
BseBI CCWGG 2 cut(s) 388, 401
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 1 cut(s) 367
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMII CTCAG 2 cut(s) 288, 368
BseNI ACTGG 1 cut(s) 113
BseXI GCAGC 2 cut(s) 362, 408
BsgI GTGCAG 1 cut(s) 369
BshFI GGCC 3 cut(s) 17, 73, 118
BsiSI CCGG 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 179
BsnI GGCC 3 cut(s) 17, 73, 118
BspANI GGCC 3 cut(s) 17, 73, 118
BspCNI CTCAG 2 cut(s) 289, 367
BspLI GGNNCC 1 cut(s) 74
BsrI ACTGG 1 cut(s) 113
BssECI CCNNGG 1 cut(s) 120
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 2 cut(s) 388, 401
Bst4CI ACNGT 1 cut(s) 293
Bst6I CTCTTC 1 cut(s) 320
BstBAI YACGTR 1 cut(s) 137
BstDEI CTNAG 2 cut(s) 297, 354
BstF5I GGATG 1 cut(s) 367
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 2 cut(s) 54, 396
BstMWI GCNNNNNNNGC 3 cut(s) 34, 58, 302
BstNI CCWGG 2 cut(s) 388, 401
BstSCI CCNGG 3 cut(s) 320, 386, 399
BstV1I GCAGC 2 cut(s) 362, 408
BstV2I GAAGAC 1 cut(s) 206
BsuRI GGCC 3 cut(s) 17, 73, 118
BtsCI GGATG 1 cut(s) 367
CfoI GCGC 2 cut(s) 54, 396
Cfr13I GGNCC 2 cut(s) 72, 117
CviJI RGCY 6 cut(s) 17, 73, 118, 189, 353, 399
CviKI_1 RGCY 6 cut(s) 17, 73, 118, 189, 353, 399
DdeI CTNAG 2 cut(s) 297, 354
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 320
EarI CTCTTC 1 cut(s) 320
Eco130I CCWWGG 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 50
Eco72I CACGTG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 72
EcoRII CCWGG 2 cut(s) 386, 399
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaiI YATR 3 cut(s) 94, 186, 272
Fnu4HI GCNGC 2 cut(s) 351, 397
FokI GGATG 1 cut(s) 374
Fsp4HI GCNGC 2 cut(s) 351, 397
FspI TGCGCA 1 cut(s) 395
GlaI GCGC 2 cut(s) 53, 395
GluI GCNGC 2 cut(s) 351, 397
GsuI CTGGAG 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 55
HaeIII GGCC 3 cut(s) 17, 73, 118
HapII CCGG 1 cut(s) 322
HhaI GCGC 2 cut(s) 54, 396
Hin6I GCGC 2 cut(s) 52, 394
HinP1I GCGC 2 cut(s) 52, 394
HinfI GANTC 3 cut(s) 131, 159, 202
HpaII CCGG 1 cut(s) 322
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 2 cut(s) 168, 193
Hpy188III TCNNGA 2 cut(s) 67, 100
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 1 cut(s) 108
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4IV ACGT 3 cut(s) 136, 249, 346
HpyCH4V TGCA 2 cut(s) 277, 350
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 58, 302
HpyF3I CTNAG 2 cut(s) 297, 354
HpySE526I ACGT 3 cut(s) 136, 249, 346
HspAI GCGC 2 cut(s) 52, 394
LpnPI CCDG 9 cut(s) 70, 89, 94, 127, 268, 335, 373, 386, 400
Lsp1109I GCAGC 2 cut(s) 362, 408
LweI GCATC 2 cut(s) 159, 264
MaeII ACGT 3 cut(s) 136, 249, 346
MaeIII GTNAC 3 cut(s) 80, 160, 370
MboII GAAGA 5 cut(s) 43, 58, 100, 211, 337
MluCI AATT 2 cut(s) 224, 233
MlyI GAGTC 2 cut(s) 168, 196
MmeI TCCRAC 2 cut(s) 240, 339
MnlI CCTC 3 cut(s) 63, 65, 224
MseI TTAA 2 cut(s) 153, 228
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 1 cut(s) 322
MspR9I CCNGG 3 cut(s) 322, 388, 401
MvaI CCWGG 2 cut(s) 388, 401
MwoI GCNNNNNNNGC 3 cut(s) 34, 58, 302
NciI CCSGG 1 cut(s) 322
NlaIV GGNNCC 1 cut(s) 74
NmuCI GTSAC 3 cut(s) 80, 160, 370
NsbI TGCGCA 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 142
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 2 cut(s) 352, 398
PleI GAGTC 2 cut(s) 167, 196
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
PpsI GAGTC 2 cut(s) 167, 196
Ppu21I YACGTR 1 cut(s) 137
Psp6I CCWGG 2 cut(s) 386, 399
PspCI CACGTG 1 cut(s) 137
PspGI CCWGG 2 cut(s) 386, 399
PspN4I GGNNCC 1 cut(s) 74
PspPI GGNCC 2 cut(s) 72, 117
RseI CAYNNNNRTG 1 cut(s) 167
SaqAI TTAA 2 cut(s) 153, 228
SatI GCNGC 2 cut(s) 351, 397
Sau96I GGNCC 2 cut(s) 72, 117
SchI GAGTC 2 cut(s) 168, 196
ScrFI CCNGG 3 cut(s) 322, 388, 401
SetI ASST 6 cut(s) 139, 216, 252, 349, 355, 392
SfaNI GCATC 2 cut(s) 159, 264
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
Sse9I AATT 2 cut(s) 224, 233
StyD4I CCNGG 3 cut(s) 320, 386, 399
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 1 cut(s) 293
TaiI ACGT 3 cut(s) 139, 252, 349
TasI AATT 2 cut(s) 224, 233
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 2 cut(s) 153, 228
Tru9I TTAA 2 cut(s) 153, 228
TseFI GTSAC 3 cut(s) 80, 160, 370
TseI GCWGC 2 cut(s) 350, 396
Tsp45I GTSAC 3 cut(s) 80, 160, 370
XapI RAATTY 2 cut(s) 224, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.