Rw6G023050

Ribosomal_L32e

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003194
Physical Location & Seq
Forward (+)
45241844 .. 45243747
1904 bp
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UTR
Exon/CDS
Intron
Rw6G023050.1

Sequence Viewer

Length: 402 bp
ATGGCCGTGCCTTTGCTGAAGAAGCAGATTGTGAAGAAGCGCCTCAAGCAGTTCAAGAGGCCCCAGAGTGACAGGAAGATCTGTGTCAAGACAAACTGGAGAAGGCCCAAGGGTATTGATTCACGTGTCAGGCGTAAGTTTAAGGGAGTCACTCTGATGCCTAACATTGGTTATGGCTCTGATAAGAAGACTCGCCACTACCTCCCCAACAAATTTAAGAAATTTGTTGTCCACAACGTCAATGAGTTGGAAGTTCTTATGATGCACAACAGGACTTACTGTGCTGAGATTGCACACAACATTTCCACCCGGAAGAGAAAGGAGATTGTTGAACGTGCAGCTCAGTTGGATGTTGTTGTCACCAACAAACTCGCCAGGTTGCGCAGCCAGGAAGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L32e

Protein Analysis

133

Amino Acids

15.77

Weight (kDa)

10.7

Isoelectric Point (pI)

65.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L32e PF01655 16 - 122 2.6e-47 Ribosomal protein L32
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 212, 221
AcuI CTGAAG 1 cut(s) 38
AcvI CACGTG 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 167
AflIII ACRYGT 1 cut(s) 124
AgsI TTSAA 2 cut(s) 55, 332
AjnI CCWGG 2 cut(s) 374, 387
AluBI AGCT 1 cut(s) 341
AluI AGCT 1 cut(s) 341
AoxI GGCC 3 cut(s) 3, 59, 104
ApeKI GCWGC 2 cut(s) 338, 384
ApoI RAATTY 2 cut(s) 212, 221
AspLEI GCGC 2 cut(s) 42, 384
AspS9I GGNCC 2 cut(s) 60, 105
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 352
BbrPI CACGTG 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 350, 396
BciT130I CCWGG 2 cut(s) 376, 389
BcnI CCSGG 1 cut(s) 310
BfoI RGCGCY 1 cut(s) 43
BglII AGATCT 1 cut(s) 78
BisI GCNGC 2 cut(s) 339, 385
BlsI GCNGC 2 cut(s) 340, 386
Bme1390I CCNGG 3 cut(s) 310, 376, 389
BmgT120I GGNCC 2 cut(s) 60, 105
BmiI GGNNCC 1 cut(s) 62
BmrFI CCNGG 3 cut(s) 310, 376, 389
BmsI GCATC 2 cut(s) 147, 252
BpiI GAAGAC 1 cut(s) 194
BpmI CTGGAG 1 cut(s) 118
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 1 cut(s) 310
BsaAI YACGTR 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 108
Bsc4I CCNNNNNNNGG 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 101
BseBI CCWGG 2 cut(s) 376, 389
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMII CTCAG 2 cut(s) 276, 356
BseNI ACTGG 1 cut(s) 101
BseXI GCAGC 2 cut(s) 350, 396
BsgI GTGCAG 1 cut(s) 357
BshFI GGCC 3 cut(s) 5, 61, 106
BsiSI CCGG 1 cut(s) 310
BslI CCNNNNNNNGG 1 cut(s) 167
BsnI GGCC 3 cut(s) 5, 61, 106
Bsp143I GATC 1 cut(s) 78
BspANI GGCC 3 cut(s) 5, 61, 106
BspCNI CTCAG 2 cut(s) 277, 355
BspLI GGNNCC 1 cut(s) 62
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 2 cut(s) 376, 389
Bst4CI ACNGT 1 cut(s) 281
Bst6I CTCTTC 1 cut(s) 308
BstBAI YACGTR 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 285, 342
BstF5I GGATG 1 cut(s) 355
BstH2I RGCGCY 1 cut(s) 43
BstHHI GCGC 2 cut(s) 42, 384
BstKTI GATC 1 cut(s) 81
BstMBI GATC 1 cut(s) 78
BstMWI GCNNNNNNNGC 3 cut(s) 22, 46, 290
BstNI CCWGG 2 cut(s) 376, 389
BstSCI CCNGG 3 cut(s) 308, 374, 387
BstV1I GCAGC 2 cut(s) 350, 396
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
BsuRI GGCC 3 cut(s) 5, 61, 106
BtsCI GGATG 1 cut(s) 355
CfoI GCGC 2 cut(s) 42, 384
Cfr13I GGNCC 2 cut(s) 60, 105
CviJI RGCY 6 cut(s) 5, 61, 106, 177, 341, 387
CviKI_1 RGCY 6 cut(s) 5, 61, 106, 177, 341, 387
DdeI CTNAG 2 cut(s) 285, 342
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco130I CCWWGG 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 38
Eco72I CACGTG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 60
EcoRII CCWGG 2 cut(s) 374, 387
EcoT14I CCWWGG 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 108
FaiI YATR 2 cut(s) 174, 260
Fnu4HI GCNGC 2 cut(s) 339, 385
FokI GGATG 1 cut(s) 362
Fsp4HI GCNGC 2 cut(s) 339, 385
FspI TGCGCA 1 cut(s) 383
GlaI GCGC 2 cut(s) 41, 383
GluI GCNGC 2 cut(s) 339, 385
GsuI CTGGAG 1 cut(s) 118
HaeII RGCGCY 1 cut(s) 43
HaeIII GGCC 3 cut(s) 5, 61, 106
HapII CCGG 1 cut(s) 310
HhaI GCGC 2 cut(s) 42, 384
Hin6I GCGC 2 cut(s) 40, 382
HinP1I GCGC 2 cut(s) 40, 382
HinfI GANTC 3 cut(s) 119, 147, 190
HpaII CCGG 1 cut(s) 310
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 2 cut(s) 156, 181
Hpy188III TCNNGA 2 cut(s) 55, 88
Hpy8I GTNNAC 1 cut(s) 232
HpyAV CCTTC 1 cut(s) 96
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 3 cut(s) 124, 237, 334
HpyCH4V TGCA 3 cut(s) 265, 293, 338
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 46, 290
HpyF3I CTNAG 2 cut(s) 285, 342
HpySE526I ACGT 3 cut(s) 124, 237, 334
HspAI GCGC 2 cut(s) 40, 382
Kzo9I GATC 1 cut(s) 78
LpnPI CCDG 9 cut(s) 58, 77, 82, 115, 256, 323, 361, 374, 388
Lsp1109I GCAGC 2 cut(s) 350, 396
LweI GCATC 2 cut(s) 147, 252
MaeII ACGT 3 cut(s) 124, 237, 334
MaeIII GTNAC 3 cut(s) 68, 148, 358
MalI GATC 1 cut(s) 80
MboI GATC 1 cut(s) 78
MboII GAAGA 5 cut(s) 31, 46, 88, 199, 325
MflI RGATCY 1 cut(s) 78
MluCI AATT 2 cut(s) 212, 221
MlyI GAGTC 2 cut(s) 156, 184
MmeI TCCRAC 2 cut(s) 228, 327
MnlI CCTC 3 cut(s) 51, 53, 212
MseI TTAA 2 cut(s) 141, 216
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 1 cut(s) 310
MspR9I CCNGG 3 cut(s) 310, 376, 389
MvaI CCWGG 2 cut(s) 376, 389
MwoI GCNNNNNNNGC 3 cut(s) 22, 46, 290
NciI CCSGG 1 cut(s) 310
NdeII GATC 1 cut(s) 78
NlaIV GGNNCC 1 cut(s) 62
NmuCI GTSAC 3 cut(s) 68, 148, 358
NsbI TGCGCA 1 cut(s) 383
PcsI WCGNNNNNNNCGW 1 cut(s) 130
PfeI GAWTC 1 cut(s) 119
PkrI GCNGC 2 cut(s) 340, 386
PleI GAGTC 2 cut(s) 155, 184
PmaCI CACGTG 1 cut(s) 125
PmlI CACGTG 1 cut(s) 125
PpsI GAGTC 2 cut(s) 155, 184
Ppu21I YACGTR 1 cut(s) 125
Psp6I CCWGG 2 cut(s) 374, 387
PspCI CACGTG 1 cut(s) 125
PspGI CCWGG 2 cut(s) 374, 387
PspN4I GGNNCC 1 cut(s) 62
PspPI GGNCC 2 cut(s) 60, 105
PsuI RGATCY 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 155
SaqAI TTAA 2 cut(s) 141, 216
SatI GCNGC 2 cut(s) 339, 385
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 2 cut(s) 60, 105
SchI GAGTC 2 cut(s) 156, 184
ScrFI CCNGG 3 cut(s) 310, 376, 389
SetI ASST 6 cut(s) 127, 204, 240, 337, 343, 380
SfaNI GCATC 2 cut(s) 147, 252
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 2 cut(s) 212, 221
StyD4I CCNGG 3 cut(s) 308, 374, 387
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 3 cut(s) 127, 240, 337
TasI AATT 2 cut(s) 212, 221
TfiI GAWTC 1 cut(s) 119
Tru1I TTAA 2 cut(s) 141, 216
Tru9I TTAA 2 cut(s) 141, 216
TseFI GTSAC 3 cut(s) 68, 148, 358
TseI GCWGC 2 cut(s) 338, 384
Tsp45I GTSAC 3 cut(s) 68, 148, 358
XapI RAATTY 2 cut(s) 212, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.