MD05G1291900.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003194
Physical Location & Seq
Forward (+)
42349927 .. 42352265
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1291900.v1.1.491

Sequence Viewer

Length: 402 bp
ATGGCGGTGCCGTTGCTGAAGAAGTCGATCGTGAAGAAGCGGGTCAAGCACTTCAAGAGGCCCCAGAGCGACCGCAAGGCCTCCGTCAAGCCCAGCTGGAGAAGACCAAAGGGTATTGATTCTCGTGTTAGAAGAAAGTTCAAGGGATGTGTTCTCATGCCCAACATTGGTTATGGGTCAGACAAGAAGACTCGCCATTATCTTCCCAATCATTTCAAGAAGTTTGTTGTGCACAACGTCAAAGAGCTTGAACTGCTGATGATGCACAACAGGACCTACTGCGCTGAGATTGCACACAATGTTTCCACAAGGAAGAGGAAAGAAATTGTTGAGAGAGCCGCGCAGCTTGATGTTGTTGTCACGAACAAGCTGGCCAGGTTGCGCAGCCAGGAAGATGAATGA

Protein Analysis

134

Amino Acids

15.66

Weight (kDa)

10.7

Isoelectric Point (pI)

59.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L32e PF01655 16 - 122 6.2e-48 Ribosomal protein L32
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 7
AccII CGCG 1 cut(s) 341
AciI CCGC 4 cut(s) 5, 40, 73, 339
AcoI YGGCCR 1 cut(s) 372
AcuI CTGAAG 1 cut(s) 38
AfiI CCNNNNNNNGG 1 cut(s) 167
AgsI TTSAA 4 cut(s) 55, 142, 217, 251
AjnI CCWGG 2 cut(s) 374, 387
AluBI AGCT 4 cut(s) 96, 247, 346, 370
AluI AGCT 4 cut(s) 96, 247, 346, 370
Alw21I GWGCWC 1 cut(s) 234
Alw44I GTGCAC 1 cut(s) 230
AoxI GGCC 3 cut(s) 59, 78, 372
ApaLI GTGCAC 1 cut(s) 230
ApeKI GCWGC 2 cut(s) 343, 384
AspLEI GCGC 3 cut(s) 284, 343, 384
AspS9I GGNCC 2 cut(s) 60, 273
AvaII GGWCC 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 234
BalI TGGCCA 1 cut(s) 374
BanI GGYRCC 1 cut(s) 7
BauI CACGAG 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 109, 194
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 2 cut(s) 355, 396
BciT130I CCWGG 2 cut(s) 376, 389
BisI GCNGC 3 cut(s) 339, 344, 385
BlsI GCNGC 3 cut(s) 340, 345, 386
Bme1390I CCNGG 2 cut(s) 376, 389
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 2 cut(s) 60, 273
BmiI GGNNCC 2 cut(s) 9, 62
BmrFI CCNGG 2 cut(s) 376, 389
BmsI GCATC 1 cut(s) 252
BpiI GAAGAC 2 cut(s) 109, 194
BpmI CTGGAG 1 cut(s) 118
Bsc4I CCNNNNNNNGG 1 cut(s) 167
BseBI CCWGG 2 cut(s) 376, 389
BseGI GGATG 1 cut(s) 152
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMII CTCAG 1 cut(s) 276
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 2 cut(s) 355, 396
BseYI CCCAGC 1 cut(s) 92
Bsh1236I CGCG 1 cut(s) 341
Bsh1285I CGRYCG 2 cut(s) 30, 73
BshFI GGCC 3 cut(s) 61, 80, 374
BshNI GGYRCC 1 cut(s) 7
BsiEI CGRYCG 2 cut(s) 30, 73
BsiHKAI GWGCWC 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 167
BsnI GGCC 3 cut(s) 61, 80, 374
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 1 cut(s) 27
BspACI CCGC 4 cut(s) 5, 40, 73, 339
BspANI GGCC 3 cut(s) 61, 80, 374
BspCNI CTCAG 1 cut(s) 277
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 2 cut(s) 9, 62
BspT107I GGYRCC 1 cut(s) 7
BssMI GATC 1 cut(s) 27
BssSI CACGAG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 123
Bst2UI CCWGG 2 cut(s) 376, 389
Bst6I CTCTTC 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 285
BstF5I GGATG 1 cut(s) 152
BstFNI CGCG 1 cut(s) 341
BstHHI GCGC 3 cut(s) 284, 343, 384
BstKTI GATC 1 cut(s) 30
BstMBI GATC 1 cut(s) 27
BstMCI CGRYCG 2 cut(s) 30, 73
BstMWI GCNNNNNNNGC 4 cut(s) 46, 253, 262, 290
BstNI CCWGG 2 cut(s) 376, 389
BstSCI CCNGG 2 cut(s) 374, 387
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 2 cut(s) 355, 396
BstV2I GAAGAC 2 cut(s) 109, 194
BsuRI GGCC 3 cut(s) 61, 80, 374
BtsCI GGATG 1 cut(s) 152
Cac8I GCNNGC 1 cut(s) 372
CfoI GCGC 3 cut(s) 284, 343, 384
Cfr13I GGNCC 2 cut(s) 60, 273
CviAII CATG 1 cut(s) 157
DdeI CTNAG 1 cut(s) 285
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 308
EarI CTCTTC 1 cut(s) 308
Eco147I AGGCCT 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 273
Eco57I CTGAAG 1 cut(s) 38
EcoO109I RGGNCCY 2 cut(s) 60, 273
EcoRII CCWGG 2 cut(s) 374, 387
FaeI CATG 1 cut(s) 160
FaiI YATR 2 cut(s) 158, 174
FatI CATG 1 cut(s) 156
FauI CCCGC 1 cut(s) 33
Fnu4HI GCNGC 3 cut(s) 339, 344, 385
FokI GGATG 1 cut(s) 159
Fsp4HI GCNGC 3 cut(s) 339, 344, 385
FspI TGCGCA 1 cut(s) 383
GlaI GCGC 3 cut(s) 283, 342, 383
GluI GCNGC 3 cut(s) 339, 344, 385
GsaI CCCAGC 1 cut(s) 96
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 3 cut(s) 61, 80, 374
HhaI GCGC 3 cut(s) 284, 343, 384
Hin1II CATG 1 cut(s) 160
Hin6I GCGC 3 cut(s) 282, 341, 382
HinP1I GCGC 3 cut(s) 282, 341, 382
HinfI GANTC 2 cut(s) 119, 190
Hpy166II GTNNAC 1 cut(s) 232
Hpy188I TCNGA 1 cut(s) 181
Hpy188III TCNNGA 4 cut(s) 31, 55, 217, 361
Hpy8I GTNNAC 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 3 cut(s) 232, 265, 293
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 253, 262, 290
HpyF3I CTNAG 1 cut(s) 285
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 1 cut(s) 160
HspAI GCGC 3 cut(s) 282, 341, 382
Kzo9I GATC 1 cut(s) 27
LpnPI CCDG 8 cut(s) 77, 82, 106, 256, 356, 361, 374, 388
Lsp1109I GCAGC 2 cut(s) 355, 396
LweI GCATC 1 cut(s) 252
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 358
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 7 cut(s) 31, 46, 114, 144, 194, 199, 325
MhlI GDGCHC 1 cut(s) 234
MlsI TGGCCA 1 cut(s) 374
MluCI AATT 1 cut(s) 324
MluNI TGGCCA 1 cut(s) 374
MlyI GAGTC 1 cut(s) 184
MnlI CCTC 3 cut(s) 51, 91, 309
Mox20I TGGCCA 1 cut(s) 374
MscI TGGCCA 1 cut(s) 374
Msp20I TGGCCA 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 96
MspR9I CCNGG 2 cut(s) 376, 389
MvaI CCWGG 2 cut(s) 376, 389
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 4 cut(s) 46, 253, 262, 290
NdeII GATC 1 cut(s) 27
NlaIII CATG 1 cut(s) 160
NlaIV GGNNCC 2 cut(s) 9, 62
NmuCI GTSAC 1 cut(s) 358
NsbI TGCGCA 1 cut(s) 383
PceI AGGCCT 1 cut(s) 80
PfeI GAWTC 1 cut(s) 119
PkrI GCNGC 3 cut(s) 340, 345, 386
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 273
Psp6I CCWGG 2 cut(s) 374, 387
PspFI CCCAGC 1 cut(s) 92
PspGI CCWGG 2 cut(s) 374, 387
PspN4I GGNNCC 2 cut(s) 9, 62
PspPI GGNCC 2 cut(s) 60, 273
PspPPI RGGWCCY 1 cut(s) 273
PvuI CGATCG 1 cut(s) 30
PvuII CAGCTG 1 cut(s) 96
SatI GCNGC 3 cut(s) 339, 344, 385
Sau3AI GATC 1 cut(s) 27
Sau96I GGNCC 2 cut(s) 60, 273
SchI GAGTC 1 cut(s) 184
ScrFI CCNGG 2 cut(s) 376, 389
SduI GDGCHC 1 cut(s) 234
SetI ASST 7 cut(s) 98, 240, 249, 278, 348, 372, 380
SfaNI GCATC 1 cut(s) 252
SinI GGWCC 1 cut(s) 273
Sse9I AATT 1 cut(s) 324
SseBI AGGCCT 1 cut(s) 80
SsiI CCGC 4 cut(s) 5, 40, 73, 339
StuI AGGCCT 1 cut(s) 80
StyD4I CCNGG 2 cut(s) 374, 387
TaiI ACGT 1 cut(s) 240
TaqI TCGA 1 cut(s) 26
TasI AATT 1 cut(s) 324
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 1 cut(s) 119
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 2 cut(s) 343, 384
Tsp45I GTSAC 1 cut(s) 358
TspGWI ACGGA 1 cut(s) 73
VneI GTGCAC 1 cut(s) 230
VpaK11BI GGWCC 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.