Rroxscaffold_1G00063690

Ribosomal_L32e

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003194
Physical Location & Seq
Forward (+)
85488393 .. 85490154
1762 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00063690.1

Sequence Viewer

Length: 402 bp
ATGGCGGTTCCGTTGTTGAAGAAGGCGATCGTGAAGAAGAGGGTCAAGCAATTTAAGAGGCCCCAAAGCGACCGCAAGATCTGCGTCAAGACCAACTGGAGGAGGCCTAAGGGTATTGACTCTCGTGTACGAAGAAAGTTCAAGGGAGTTACTCTCATGCCCAACATTGGTTATGGTTCTGACAAGAAGACTCGCCATTATCTTCCCAATCATTTCAAGAAGTTTGTTGTGCACAATGTCAAGGAGCTAGAAGTTCTGATGATGCACAATAGGACCTATTGTGCCGAGATAGCACACAATATCTCCACAAGGAAGAGGAAGGAAATTGTTGAGAGAGCCGCGCAGCTTGACGTTGTTGTCACTAACAAGCTTGCTAGGTTGCGTAGCCAAGAGGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L32e

Protein Analysis

133

Amino Acids

15.72

Weight (kDa)

10.7

Isoelectric Point (pI)

61.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L32e PF01655 16 - 122 1e-47 Ribosomal protein L32
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 356
AccII CGCG 1 cut(s) 341
AciI CCGC 3 cut(s) 5, 73, 339
AfaI GTAC 1 cut(s) 129
AfiI CCNNNNNNNGG 2 cut(s) 99, 167
AgsI TTSAA 3 cut(s) 19, 142, 217
AluBI AGCT 3 cut(s) 247, 346, 370
AluI AGCT 3 cut(s) 247, 346, 370
Alw21I GWGCWC 1 cut(s) 234
Alw44I GTGCAC 1 cut(s) 230
AoxI GGCC 2 cut(s) 59, 104
ApaLI GTGCAC 1 cut(s) 230
ApeKI GCWGC 1 cut(s) 343
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 60, 273
AvaII GGWCC 1 cut(s) 273
AxyI CCTNAGG 1 cut(s) 108
BaeGI GKGCMC 1 cut(s) 234
BauI CACGAG 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 355
BfaI CTAG 2 cut(s) 248, 375
BglII AGATCT 1 cut(s) 78
BisI GCNGC 2 cut(s) 339, 344
BlsI GCNGC 2 cut(s) 340, 345
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 2 cut(s) 60, 273
BmiI GGNNCC 2 cut(s) 9, 62
BmsI GCATC 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 194
BplI GAGNNNNNCTC 2 cut(s) 138, 170
BpmI CTGGAG 1 cut(s) 118
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 2 cut(s) 99, 167
Bse1I ACTGG 1 cut(s) 101
Bse21I CCTNAGG 1 cut(s) 108
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 2 cut(s) 99, 167
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 1 cut(s) 115
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 341
Bsh1285I CGRYCG 2 cut(s) 30, 73
BshFI GGCC 2 cut(s) 61, 106
BsiEI CGRYCG 2 cut(s) 30, 73
BsiHKAI GWGCWC 1 cut(s) 234
BslI CCNNNNNNNGG 2 cut(s) 99, 167
BsnI GGCC 2 cut(s) 61, 106
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 2 cut(s) 27, 78
BspACI CCGC 3 cut(s) 5, 73, 339
BspANI GGCC 2 cut(s) 61, 106
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 2 cut(s) 9, 62
BsrI ACTGG 1 cut(s) 101
BssMI GATC 2 cut(s) 27, 78
BssSI CACGAG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 123
Bst6I CTCTTC 2 cut(s) 32, 308
BstAPI GCANNNNNTGC 1 cut(s) 81
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 108
BstF5I GGATG 1 cut(s) 400
BstFNI CGCG 1 cut(s) 341
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 2 cut(s) 30, 81
BstMBI GATC 2 cut(s) 27, 78
BstMCI CGRYCG 2 cut(s) 30, 73
BstMWI GCNNNNNNNGC 2 cut(s) 81, 290
BstSLI GKGCMC 1 cut(s) 234
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 1 cut(s) 355
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
Bsu36I CCTNAGG 1 cut(s) 108
BsuRI GGCC 2 cut(s) 61, 106
BtsCI GGATG 1 cut(s) 400
Cac8I GCNNGC 1 cut(s) 372
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 60, 273
CseI GACGC 1 cut(s) 73
Csp6I GTAC 1 cut(s) 128
CviAII CATG 1 cut(s) 157
CviJI RGCY 7 cut(s) 61, 106, 247, 338, 346, 370, 387
CviKI_1 RGCY 7 cut(s) 61, 106, 247, 338, 346, 370, 387
CviQI GTAC 1 cut(s) 128
DdeI CTNAG 1 cut(s) 108
DpnI GATC 2 cut(s) 29, 80
DpnII GATC 2 cut(s) 27, 78
DrdI GACNNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 356
Eam1104I CTCTTC 2 cut(s) 32, 308
EarI CTCTTC 2 cut(s) 32, 308
Eco147I AGGCCT 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 273
Eco81I CCTNAGG 1 cut(s) 108
EcoO109I RGGNCCY 2 cut(s) 60, 273
FaeI CATG 1 cut(s) 160
FaiI YATR 2 cut(s) 158, 174
FatI CATG 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 339, 344
Fsp4HI GCNGC 2 cut(s) 339, 344
FspBI CTAG 2 cut(s) 248, 375
GlaI GCGC 1 cut(s) 342
GluI GCNGC 2 cut(s) 339, 344
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 2 cut(s) 61, 106
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 343
Hin1II CATG 1 cut(s) 160
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 2 cut(s) 119, 190
Hpy166II GTNNAC 2 cut(s) 128, 232
Hpy188I TCNGA 2 cut(s) 181, 258
Hpy188III TCNNGA 3 cut(s) 31, 88, 217
Hpy8I GTNNAC 2 cut(s) 128, 232
HpyAV CCTTC 2 cut(s) 16, 313
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 2 cut(s) 232, 265
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 290
HpyF3I CTNAG 1 cut(s) 108
HpySE526I ACGT 1 cut(s) 351
Hsp92II CATG 1 cut(s) 160
HspAI GCGC 1 cut(s) 341
Kzo9I GATC 2 cut(s) 27, 78
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 1 cut(s) 82
Lsp1109I GCAGC 1 cut(s) 355
LweI GCATC 1 cut(s) 252
MaeI CTAG 2 cut(s) 248, 375
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 2 cut(s) 148, 358
MalI GATC 2 cut(s) 29, 80
MboI GATC 2 cut(s) 27, 78
MboII GAAGA 7 cut(s) 31, 46, 49, 144, 194, 199, 325
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 2 cut(s) 50, 324
MlyI GAGTC 2 cut(s) 113, 184
MnlI CCTC 6 cut(s) 33, 51, 93, 96, 309, 385
MseI TTAA 1 cut(s) 54
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 2 cut(s) 81, 290
NdeII GATC 2 cut(s) 27, 78
NlaIII CATG 1 cut(s) 160
NlaIV GGNNCC 2 cut(s) 9, 62
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 358
PceI AGGCCT 1 cut(s) 106
PkrI GCNGC 2 cut(s) 340, 345
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 2 cut(s) 113, 184
PpsI GAGTC 2 cut(s) 113, 184
PpuMI RGGWCCY 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 273
PspN4I GGNNCC 2 cut(s) 9, 62
PspPI GGNCC 2 cut(s) 60, 273
PspPPI RGGWCCY 1 cut(s) 273
PsuI RGATCY 1 cut(s) 78
PvuI CGATCG 1 cut(s) 30
RsaI GTAC 1 cut(s) 129
RsaNI GTAC 1 cut(s) 128
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 2 cut(s) 339, 344
Sau3AI GATC 2 cut(s) 27, 78
Sau96I GGNCC 2 cut(s) 60, 273
SchI GAGTC 2 cut(s) 113, 184
SduI GDGCHC 1 cut(s) 234
SetI ASST 6 cut(s) 249, 278, 348, 354, 372, 380
SfaNI GCATC 1 cut(s) 252
SinI GGWCC 1 cut(s) 273
Sse9I AATT 2 cut(s) 50, 324
SseBI AGGCCT 1 cut(s) 106
SsiI CCGC 3 cut(s) 5, 73, 339
SspMI CTAG 2 cut(s) 248, 375
StuI AGGCCT 1 cut(s) 106
TaiI ACGT 1 cut(s) 354
TasI AATT 2 cut(s) 50, 324
TauI GCSGC 1 cut(s) 341
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 1 cut(s) 343
Tsp45I GTSAC 1 cut(s) 358
VneI GTGCAC 1 cut(s) 230
VpaK11BI GGWCC 1 cut(s) 273
XspI CTAG 2 cut(s) 248, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.