Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0006171

Physical Location & Seq

Genomic Coordinates Forward (+)

5370297 .. 5373481

Gene Structure 3185 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g09200.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2574 bp

ATGGGAAAAGTTGTTGGCATTAAGATGTCTGTTGATTTGGAGAGTGTGAATGGAAATGTAATTAAAGGTGTAGACTCAAAGAATCTGTCAAAGATCCGTCCCATTTCAGTGGGGGATTTTGTTGTTTGTGGGCCCTGGCTTGGAAGGGTGGACAGAGTAGTTGACTCTGTAGCTATTGTATTTGACGATGGCACAGAGTGTATGGTGACTGCTGTGGATCCAGAGAAGCTCGTACCTGTCTCCCCAAACATACTTGAAGATCCACAGTATCCATATTATCCAGGACAGAGAGTACAGGTTAGGCTCTCAACTGTTTCCAAATCAACTAGATGGTTATGTGGTAACTGGAGGGAAAATCAAGATGAGGGAACTGTTTGTTCTTTGGAAGGTGGTGTAGTGTATGTTGATTGGCTTGCCTCTGTTCTCGTAGGTTGTGATTCGAATTTTCCCACTCCTCCACGTCAGCTGGATTCGAAAAAATTGACTTTGGTATCATGTTTCTCTCATGCCAATTGGCAGCTTGGTGACTGGTGCATGCTTGCAATTGCTGAGTGTGAAGGTTTCTCAGAGCAGGCTTTTCATAGAACTTCGACTTGTGAGCTCACCAACAAGCAAGAAAATTCAGAAAGAGGATTTAAGAGAAGAAATCCCAAGTCAAAAATTGAGGAAATCTTTGTAATCTCAAAGACAAAGACCAAAGTTAATGTTCTGTGGCAAGATGGTAGCCAGTCTTTGGAAGTGGATTCACAGACGTTAGTTCCGGTCAGTGCCATAAATGATCATGAATTTTGGCCTGAACAGTTTGTCCTTGAAAAGGGAACTTATGATGATCCAAACTTATTCAGCACCCAAAGGTGGGGTGTTGTGCGTGGTGTGGATGCAAAGGAACGAACAGTAAAGGTGGAATGGAAAACTATAAGCGCATCTGGAGCAAATAGTTTAGAGGGAGAGCAGATGGAGGAATTTGTAAGTGCTTATGAGCTTGTTGAGCACCCGGATTACTCCTACTGTTTTGGAGATTATGTGTTCAGGATGGTCCAGAATCAGTTTGATGAACAACCTGATAAAAACTATCTGAGCTTAAGGTCTGATATGACTGAGGAGGTAGTTCCGGAAGAGAAGAACTTTGGTGGGGATCAAGAAACTTTCACAGATAAAAGTTACCTACCTTGCATTGGCAATGTTTTGCGCTTCATAGATGGTGCGGTAGAAGTGAGATGGGCAACTGGCATTACTAGCAAGGTGGCCCCTTGTGAAATATTGCGGTTTGATAAACATGAAGGTTCAGCTGCAATCAGTGTAGAAGAGGACACTGAGGAATTGAACAAAGAGATAATTCCACATGGAAAACAAATTGATAATGAGAAAGGAAAGGATTCCTTGAATTTAGATGTTGCTGCTAAAAGTTGCAAACAGCACACAAGCGAGTCCAGTTCCTATTTCCTTCCTCAAGCTGCCATTGGATTTTTCTCTAGCATTGCTGTGAGTCTCTTTGGATCCCATGGTTACACAGCACTTTCAGGTCCTTCCTCATCAGTCTGTACTTCTGAAGTTGGAAAGGAATCTGGGTTCTGCCATGCGAAAAGAATAGTTGAAAATCATGATATCTGCACTGAGCCACATTCAATGTATGAGTCTGATATTTCTGGCAATGAAAATTTGAATCTTGAAGGCAAAGACATTGAGGGGAGTAGATATCTTCCATATTTGAAAGACAAGGAGAGTGCAGACCAGTTCAGGCAGTTTGACATGCTTGCTGATTGTGCAGATCACTATTTTCATGGTGCCAGCAAGGAGTTGGCATTATCTCAGGTGAAAAAAGGTTGGATGAAGAAGGTTCAGCAAGAGTGGAGCACCTTAGAGAAAGATCTTCCTGAACAAATCTATGTTCGTGCCTTTGAGGAAAGGATGGATCTACTGAGGGCAGCCATCGTTGGTGCACCAGGAACTCCATATCATGATGGGCTTTTCTTCTTTGACATTTATCTTCCACCAGAGTATCCTCATGAGCCACCAATGGTACACTACATTTCTGGTGGGCTACGTGTGAACCCGAACTTGTATGAGTCTGGAAAGGTCTGCCTCAGTCTTCTTAACACGTGGACTGGTACGGGCAGTGAAGTTTGGAATCCTGGGAGCTCCACCATTCTTCAAGTTCTTCTCTCCCTTCAGGCCCTCGTGCTTAACGATAGGCCATATTTCAATGAAGCTGGATATGATCAGCAGGTGGGAAGAACTGAGGGAGAGAGAAACTCAGTGAGCTACAATGAAAATGCATTCCTTATGACTTGCAAGTCAATGCTGTATTCTCTCCAAAAGCCGCCTATGCATTTTGAAGGACTTGTGATGGAACACTTCACTCGGCACTCTGAGAATATCCTAATGGCTTGTAAGGCATATATGGAGGGGGTTCCAATAGGATGTGCTGTTGATTTTCAGAAAACCGATGATAAACACAGTAAGGGAAGCTCTACTGGATTCAAGATTATGCTAGCTAAGCTCTTTCAGAAACTAGTGGAGTCGTTTTCTGCCAAAGGAATCGACTGCAGCCAGTTCATTGGAGCAGTGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006464 GO:0006807 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0010966 GO:0012505 GO:0015698 GO:0016036 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0030163 GO:0031625 GO:0031667 GO:0031668 GO:0031669 GO:0032446 GO:0032879 GO:0033554 GO:0034762 GO:0034765 GO:0036211 GO:0042592 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050789 GO:0050801 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0055062 GO:0055081 GO:0055083 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070647 GO:0071496 GO:0071704 GO:0072505 GO:0072506 GO:0098771 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903795 GO:1903959 GO:2000185

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

858

Amino Acids

95.97

Weight (kDa)

5.05

Isoelectric Point (pI)

40.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tSH3-B_UBE2O	PF23046	37 - 169	1.5e-40	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	260 - 333	9.2e-18	UBE2O, SH3-B domain
SH3-C_UBE2O	PF23044	384 - 426	6.5e-06	UBE2O, ubiquitin binding SH3-C domain
UQ_con	PF00179	613 - 746	8.7e-24	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33770
fragaria_vesca	FvH4_3g09200 FvH4_3g29950
malus_domestica	MD05G1312700.v1.1 MD10G1294500.v1.1
prunus_persica	Prupe.4G047400_v2.0.a1 Prupe.4G047400_v2.0.a1
pyrus_communis	pycom05g29070 pycom05g29080 pycom10g24620 pycom10g24630 pycom10g24640
rosa_chinensis	RchiOBHm_Chr5g0008401
rosa_laevigata	RLG00000031568
rosa_multiflora	Rmu_co8007308.1_g000001 Rmu_co8292313.1_g000001 Rmu_sc0004326.1_g000003 Rmu_sc0028200.1_g000001 Rmu_ssc0000388.1_g000061
rosa_roxburghii	Rroxscaffold_1G00068090
rosa_rugosa	Rorug04G0435600
rosa_samantha	Rh5AG064200 Rh5BG061400 Rh5CG071300 Rh5DG060200 Rh5DG060300
rosa_wichuraiana	Rw5G005660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2215
AasI	GACNNNNNNGTC	1 cut(s)	2293
Acc36I	ACCTGC	1 cut(s)	2215
AccB1I	GGYRCC	1 cut(s)	1784
AccB7I	CCANNNNNTGG	1 cut(s)	733
AccI	GTMKAC	1 cut(s)	72
AccIII	TCCGGA	1 cut(s)	1111
AciI	CCGC	3 cut(s)	1205, 1264, 2321
AclWI	GGATC	9 cut(s)	88, 212, 225, 254, 824, 1143, 1491, 1504, 1920
AcsI	RAATTY	6 cut(s)	442, 619, 785, 962, 1384, 1657
AcuI	CTGAAG	2 cut(s)	1569, 2153
AcvI	CACGTG	1 cut(s)	2100
AdeI	CACNNNGTG	1 cut(s)	198
AfaI	GTAC	5 cut(s)	234, 294, 1543, 2022, 2110
AfiI	CCNNNNNNNGG	6 cut(s)	140, 733, 814, 855, 856, 1175
AflII	CTTAAG	1 cut(s)	1081
AflIII	ACRYGT	2 cut(s)	2044, 2097
AhlI	ACTAGT	1 cut(s)	2512
AjiI	CACGTC	1 cut(s)	461
AjnI	CCWGG	4 cut(s)	134, 280, 1942, 2131
AloI	GAACNNNNNNTCC	4 cut(s)	789, 821, 1553, 1585
Alw21I	GWGCWC	5 cut(s)	603, 993, 1856, 1942, 2141
Alw26I	GTCTC	2 cut(s)	244, 1493
Alw44I	GTGCAC	1 cut(s)	1938
AlwI	GGATC	9 cut(s)	88, 212, 225, 254, 824, 1143, 1491, 1504, 1920
Aor13HI	TCCGGA	1 cut(s)	1111
AoxI	GGCC	5 cut(s)	131, 791, 1245, 2172, 2192
ApaI	GGGCCC	1 cut(s)	135
ApaLI	GTGCAC	1 cut(s)	1938
ApeKI	GCWGC	6 cut(s)	517, 1289, 1397, 1454, 1925, 2547
ApoI	RAATTY	6 cut(s)	442, 619, 785, 962, 1384, 1657
ArsI	GACNNNNNNTTYG	2 cut(s)	71, 103
Asp700I	GAANNNNTTC	1 cut(s)	1375
AspLEI	GCGC	2 cut(s)	923, 1191
AspS9I	GGNCC	6 cut(s)	131, 132, 1036, 1246, 1523, 2173
AsuC2I	CCSGG	1 cut(s)	995
AsuHPI	GGTGA	4 cut(s)	217, 536, 595, 1825
AsuII	TTCGAA	2 cut(s)	440, 473
AsuNHI	GCTAGC	1 cut(s)	2491
AvaII	GGWCC	2 cut(s)	1036, 1523
BaeGI	GKGCMC	2 cut(s)	135, 1942
BaeI	ACNNNNGTAYC	4 cut(s)	474, 507, 1982, 2015
BamHI	GGATCC	2 cut(s)	217, 1496
BanI	GGYRCC	1 cut(s)	1784
BanII	GRGCYC	3 cut(s)	135, 603, 2141
BauI	CACGAG	1 cut(s)	2177
BbrPI	CACGTG	1 cut(s)	2100
BbsI	GAAGAC	1 cut(s)	2081
Bbv12I	GWGCWC	5 cut(s)	603, 993, 1856, 1942, 2141
BbvI	GCAGC	6 cut(s)	529, 1276, 1384, 1441, 1937, 2559
BciT130I	CCWGG	4 cut(s)	136, 282, 1944, 2133
BciVI	GTATCC	2 cut(s)	279, 2010
BclI	TGATCA	2 cut(s)	778, 2218
BcnI	CCSGG	1 cut(s)	995
BcoDI	GTCTC	2 cut(s)	244, 1493
BcuI	ACTAGT	1 cut(s)	2512
BfaI	CTAG	5 cut(s)	327, 1236, 1473, 2492, 2513
BfmI	CTRYAG	2 cut(s)	168, 2545
BfrI	CTTAAG	1 cut(s)	1081
BfuAI	ACCTGC	1 cut(s)	2215
BfuI	GTATCC	2 cut(s)	279, 2010
BglII	AGATCT	1 cut(s)	1867
BisI	GCNGC	7 cut(s)	518, 1290, 1398, 1455, 1926, 2321, 2548
BlpI	GCTNAGC	1 cut(s)	2496
BlsI	GCNGC	7 cut(s)	519, 1291, 1399, 1456, 1927, 2322, 2549
Bme1390I	CCNGG	5 cut(s)	136, 282, 995, 1944, 2133
Bme18I	GGWCC	2 cut(s)	1036, 1523
BmgBI	CACGTC	1 cut(s)	461
BmgT120I	GGNCC	6 cut(s)	131, 132, 1036, 1246, 1523, 2173
BmiI	GGNNCC	6 cut(s)	133, 219, 1248, 1498, 1786, 2412
BmrFI	CCNGG	5 cut(s)	136, 282, 995, 1944, 2133
BmsI	GCATC	2 cut(s)	868, 932
BmtI	GCTAGC	1 cut(s)	2495
BpiI	GAAGAC	1 cut(s)	2081
BplI	GAGNNNNNCTC	2 cut(s)	2237, 2269
BpmI	CTGGAG	2 cut(s)	367, 948
Bpu1102I	GCTNAGC	1 cut(s)	2496
Bpu14I	TTCGAA	2 cut(s)	440, 473
BpuEI	CTTGAG	1 cut(s)	1434
BpuMI	CCSGG	1 cut(s)	995
BsaAI	YACGTR	2 cut(s)	2045, 2100
BsaJI	CCNNGG	3 cut(s)	134, 1501, 2132
BsaWI	WCCGGW	2 cut(s)	760, 1111
BsaXI	ACNNNNNCTCC	2 cut(s)	1008, 1038
Bsc4I	CCNNNNNNNGG	6 cut(s)	140, 733, 814, 855, 856, 1175
Bse1I	ACTGG	9 cut(s)	350, 533, 727, 1231, 1431, 1732, 2110, 2479, 2551
Bse3DI	GCAATG	3 cut(s)	1186, 1476, 1657
BseAI	TCCGGA	1 cut(s)	1111
BseBI	CCWGG	4 cut(s)	136, 282, 1944, 2133
BseDI	CCNNGG	3 cut(s)	134, 1501, 2132
BseGI	GGATG	5 cut(s)	883, 1038, 1833, 1914, 2426
BseLI	CCNNNNNNNGG	6 cut(s)	140, 733, 814, 855, 856, 1175
BseMI	GCAATG	3 cut(s)	1186, 1476, 1657
BseNI	ACTGG	9 cut(s)	350, 533, 727, 1231, 1431, 1732, 2110, 2479, 2551
BseRI	GAGGAG	2 cut(s)	444, 1115
BseSI	GKGCMC	2 cut(s)	135, 1942
BseXI	GCAGC	6 cut(s)	529, 1276, 1384, 1441, 1937, 2559
BsgI	GTGCAG	3 cut(s)	1594, 1746, 1785
BshFI	GGCC	5 cut(s)	133, 793, 1247, 2174, 2194
BshNI	GGYRCC	1 cut(s)	1784
BsiHKAI	GWGCWC	5 cut(s)	603, 993, 1856, 1942, 2141
BsiSI	CCGG	3 cut(s)	761, 995, 1112
BslFI	GGGAC	1 cut(s)	84
BslI	CCNNNNNNNGG	6 cut(s)	140, 733, 814, 855, 856, 1175
BsmAI	GTCTC	2 cut(s)	244, 1493
BsmFI	GGGAC	1 cut(s)	84
BsmI	GAATGC	1 cut(s)	2276
BsnI	GGCC	5 cut(s)	133, 793, 1247, 2174, 2194
Bsp119I	TTCGAA	2 cut(s)	440, 473
Bsp120I	GGGCCC	1 cut(s)	131
Bsp1286I	GDGCHC	6 cut(s)	135, 603, 993, 1856, 1942, 2141
Bsp13I	TCCGGA	1 cut(s)	1111
Bsp1720I	GCTNAGC	1 cut(s)	2496
Bsp19I	CCATGG	1 cut(s)	1501
BspACI	CCGC	3 cut(s)	1205, 1264, 2321
BspANI	GGCC	5 cut(s)	133, 793, 1247, 2174, 2194
BspEI	TCCGGA	1 cut(s)	1111
BspHI	TCATGA	4 cut(s)	781, 1600, 1957, 2005
BspLI	GGNNCC	6 cut(s)	133, 219, 1248, 1498, 1786, 2412
BspMAI	CTGCAG	1 cut(s)	2549
BspMI	ACCTGC	1 cut(s)	2215
BspOI	GCTAGC	1 cut(s)	2495
BspPI	GGATC	9 cut(s)	88, 212, 225, 254, 824, 1143, 1491, 1504, 1920
BspT104I	TTCGAA	2 cut(s)	440, 473
BspT107I	GGYRCC	1 cut(s)	1784
BspTI	CTTAAG	1 cut(s)	1081
BsrDI	GCAATG	3 cut(s)	1186, 1476, 1657
BsrI	ACTGG	9 cut(s)	350, 533, 727, 1231, 1431, 1732, 2110, 2479, 2551
BssECI	CCNNGG	3 cut(s)	134, 1501, 2132
BssSI	CACGAG	1 cut(s)	2177
BssT1I	CCWWGG	1 cut(s)	1501
Bst2BI	CACGAG	1 cut(s)	2177
Bst2UI	CCWGG	4 cut(s)	136, 282, 1944, 2133
Bst4CI	ACNGT	7 cut(s)	267, 313, 373, 801, 895, 1010, 2459
Bst6I	CTCTTC	2 cut(s)	1110, 1299
BstAFI	CTTAAG	1 cut(s)	1081
BstBAI	YACGTR	2 cut(s)	2045, 2100
BstBI	TTCGAA	2 cut(s)	440, 473
BstC8I	GCNNGC	7 cut(s)	414, 536, 540, 573, 1755, 1789, 2493
BstDSI	CCRYGG	1 cut(s)	1501
BstENI	CCTNNNNNAGG	1 cut(s)	812
BstF5I	GGATG	5 cut(s)	883, 1038, 1833, 1914, 2426
BstHHI	GCGC	2 cut(s)	923, 1191
BstMAI	GTCTC	2 cut(s)	244, 1493
BstMWI	GCNNNNNNNGC	7 cut(s)	929, 988, 1236, 1763, 2326, 2393, 2497
BstNI	CCWGG	4 cut(s)	136, 282, 1944, 2133
BstNSI	RCATGY	2 cut(s)	538, 1753
BstSCI	CCNGG	5 cut(s)	134, 280, 993, 1942, 2131
BstSFI	CTRYAG	2 cut(s)	168, 2545
BstSLI	GKGCMC	2 cut(s)	135, 1942
BstV1I	GCAGC	6 cut(s)	529, 1276, 1384, 1441, 1937, 2559
BstV2I	GAAGAC	1 cut(s)	2081
BstX2I	RGATCY	6 cut(s)	93, 217, 259, 1496, 1867, 1912
BstXI	CCANNNNNNTGG	2 cut(s)	109, 2558
BstYI	RGATCY	6 cut(s)	93, 217, 259, 1496, 1867, 1912
BsuI	GTATCC	2 cut(s)	279, 2010
BsuRI	GGCC	5 cut(s)	133, 793, 1247, 2174, 2194
BtgI	CCRYGG	1 cut(s)	1501
BtrI	CACGTC	1 cut(s)	461
BtsCI	GGATG	5 cut(s)	883, 1038, 1833, 1914, 2426
BtsI	GCAGTG	2 cut(s)	2122, 2571
BtsIMutI	CAGTG	8 cut(s)	114, 772, 1303, 1311, 1611, 2122, 2262, 2571
BveI	ACCTGC	1 cut(s)	2215
Cac8I	GCNNGC	7 cut(s)	414, 536, 540, 573, 1755, 1789, 2493
CciI	TCATGA	4 cut(s)	781, 1600, 1957, 2005
CfoI	GCGC	2 cut(s)	923, 1191
Cfr13I	GGNCC	6 cut(s)	131, 132, 1036, 1246, 1523, 2173
Csp6I	GTAC	5 cut(s)	233, 293, 1542, 2021, 2109
CviQI	GTAC	5 cut(s)	233, 293, 1542, 2021, 2109
DraIII	CACNNNGTG	1 cut(s)	198
DrdI	GACNNNNNNGTC	1 cut(s)	2293
DseDI	GACNNNNNNGTC	1 cut(s)	2293
Eam1104I	CTCTTC	2 cut(s)	1110, 1299
EarI	CTCTTC	2 cut(s)	1110, 1299
Ecl136II	GAGCTC	2 cut(s)	601, 2139
Eco130I	CCWWGG	1 cut(s)	1501
Eco24I	GRGCYC	3 cut(s)	135, 603, 2141
Eco32I	GATATC	2 cut(s)	1606, 1697
Eco47I	GGWCC	2 cut(s)	1036, 1523
Eco53kI	GAGCTC	2 cut(s)	601, 2139
Eco57I	CTGAAG	2 cut(s)	1569, 2153
Eco72I	CACGTG	1 cut(s)	2100
EcoICRI	GAGCTC	2 cut(s)	601, 2139
EcoNI	CCTNNNNNAGG	1 cut(s)	812
EcoO109I	RGGNCCY	3 cut(s)	132, 1523, 2173
EcoRII	CCWGG	4 cut(s)	134, 280, 1942, 2131
EcoRV	GATATC	2 cut(s)	1606, 1697
EcoT14I	CCWWGG	1 cut(s)	1501
EcoT22I	ATGCAT	2 cut(s)	2278, 2331
EcoT38I	GRGCYC	3 cut(s)	135, 603, 2141
ErhI	CCWWGG	1 cut(s)	1501
FalI	AAGNNNNNCTT	2 cut(s)	1364, 1396
FaqI	GGGAC	1 cut(s)	84
FbaI	TGATCA	2 cut(s)	778, 2218
FblI	GTMKAC	1 cut(s)	72
Fnu4HI	GCNGC	7 cut(s)	518, 1290, 1398, 1455, 1926, 2321, 2548
FokI	GGATG	5 cut(s)	890, 1045, 1840, 1921, 2433
FriOI	GRGCYC	3 cut(s)	135, 603, 2141
Fsp4HI	GCNGC	7 cut(s)	518, 1290, 1398, 1455, 1926, 2321, 2548
FspBI	CTAG	5 cut(s)	327, 1236, 1473, 2492, 2513
GlaI	GCGC	2 cut(s)	922, 1190
GluI	GCNGC	7 cut(s)	518, 1290, 1398, 1455, 1926, 2321, 2548
GsuI	CTGGAG	2 cut(s)	367, 948
HaeIII	GGCC	5 cut(s)	133, 793, 1247, 2174, 2194
HapII	CCGG	3 cut(s)	761, 995, 1112
HhaI	GCGC	2 cut(s)	923, 1191
Hin6I	GCGC	2 cut(s)	921, 1189
HinP1I	GCGC	2 cut(s)	921, 1189
HincII	GTYRAC	1 cut(s)	163
HindII	GTYRAC	1 cut(s)	163
HpaII	CCGG	3 cut(s)	761, 995, 1112
HphI	GGTGA	4 cut(s)	217, 536, 595, 1825
Hpy166II	GTNNAC	7 cut(s)	73, 151, 163, 1940, 2023, 2050, 2103
Hpy188I	TCNGA	9 cut(s)	568, 625, 1077, 1090, 1549, 1639, 2371, 2439, 2508
Hpy8I	GTNNAC	7 cut(s)	73, 151, 163, 1940, 2023, 2050, 2103
HpyCH4III	ACNGT	7 cut(s)	267, 313, 373, 801, 895, 1010, 2459
HpyCH4IV	ACGT	4 cut(s)	460, 752, 2044, 2099
HpyF10VI	GCNNNNNNNGC	7 cut(s)	929, 988, 1236, 1763, 2326, 2393, 2497
HpySE526I	ACGT	4 cut(s)	460, 752, 2044, 2099
HspAI	GCGC	2 cut(s)	921, 1189
Kpn2I	TCCGGA	1 cut(s)	1111
Ksp22I	TGATCA	2 cut(s)	778, 2218
LmnI	GCTCC	5 cut(s)	929, 1851, 2136, 2144, 2561
Lsp1109I	GCAGC	6 cut(s)	529, 1276, 1384, 1441, 1937, 2559
LweI	GCATC	2 cut(s)	868, 932
MaeI	CTAG	5 cut(s)	327, 1236, 1473, 2492, 2513
MaeII	ACGT	4 cut(s)	460, 752, 2044, 2099
MaeIII	GTNAC	5 cut(s)	205, 341, 524, 1160, 1505
MfeI	CAATTG	2 cut(s)	511, 543
MflI	RGATCY	6 cut(s)	93, 217, 259, 1496, 1867, 1912
MhlI	GDGCHC	6 cut(s)	135, 603, 993, 1856, 1942, 2141
MlyI	GAGTC	7 cut(s)	68, 158, 1436, 1495, 1643, 2075, 2528
MmeI	TCCRAC	2 cut(s)	1534, 1805
Mph1103I	ATGCAT	2 cut(s)	2278, 2331
MroI	TCCGGA	1 cut(s)	1111
MroXI	GAANNNNTTC	1 cut(s)	1375
MseI	TTAA	7 cut(s)	21, 63, 636, 702, 1082, 2094, 2184
MslI	CAYNNNNRTG	3 cut(s)	23, 107, 1481
MspA1I	CMGCKG	2 cut(s)	466, 1289
MspCI	CTTAAG	1 cut(s)	1081
MspI	CCGG	3 cut(s)	761, 995, 1112
MspR9I	CCNGG	5 cut(s)	136, 282, 995, 1944, 2133
MunI	CAATTG	2 cut(s)	511, 543
Mva1269I	GAATGC	1 cut(s)	2276
MvaI	CCWGG	4 cut(s)	136, 282, 1944, 2133
MwoI	GCNNNNNNNGC	7 cut(s)	929, 988, 1236, 1763, 2326, 2393, 2497
NciI	CCSGG	1 cut(s)	995
NcoI	CCATGG	1 cut(s)	1501
NheI	GCTAGC	1 cut(s)	2491
NlaIV	GGNNCC	6 cut(s)	133, 219, 1248, 1498, 1786, 2412
NmeAIII	GCCGAG	1 cut(s)	2341
NmuCI	GTSAC	2 cut(s)	205, 524
NsiI	ATGCAT	2 cut(s)	2278, 2331
NspI	RCATGY	2 cut(s)	538, 1753
NspV	TTCGAA	2 cut(s)	440, 473
PaeI	GCATGC	1 cut(s)	538
PagI	TCATGA	4 cut(s)	781, 1600, 1957, 2005
PaqCI	CACCTGC	1 cut(s)	2215
PcsI	WCGNNNNNNNCGW	1 cut(s)	2184
PctI	GAATGC	1 cut(s)	2276
PdmI	GAANNNNTTC	1 cut(s)	1375
PflMI	CCANNNNNTGG	1 cut(s)	733
PfoI	TCCNGGA	1 cut(s)	280
PkrI	GCNGC	7 cut(s)	519, 1291, 1399, 1456, 1927, 2322, 2549
PleI	GAGTC	7 cut(s)	68, 158, 1435, 1494, 1642, 2074, 2527
PmaCI	CACGTG	1 cut(s)	2100
PmlI	CACGTG	1 cut(s)	2100
PpsI	GAGTC	7 cut(s)	68, 158, 1435, 1494, 1642, 2074, 2527
Ppu21I	YACGTR	2 cut(s)	2045, 2100
PpuMI	RGGWCCY	1 cut(s)	1523
Psp124BI	GAGCTC	2 cut(s)	603, 2141
Psp5II	RGGWCCY	1 cut(s)	1523
Psp6I	CCWGG	4 cut(s)	134, 280, 1942, 2131
PspCI	CACGTG	1 cut(s)	2100
PspGI	CCWGG	4 cut(s)	134, 280, 1942, 2131
PspN4I	GGNNCC	6 cut(s)	133, 219, 1248, 1498, 1786, 2412
PspOMI	GGGCCC	1 cut(s)	131
PspPI	GGNCC	6 cut(s)	131, 132, 1036, 1246, 1523, 2173
PspPPI	RGGWCCY	1 cut(s)	1523
PstI	CTGCAG	1 cut(s)	2549
PsuI	RGATCY	6 cut(s)	93, 217, 259, 1496, 1867, 1912
PvuII	CAGCTG	2 cut(s)	466, 1289
RsaI	GTAC	5 cut(s)	234, 294, 1543, 2022, 2110
RsaNI	GTAC	5 cut(s)	233, 293, 1542, 2021, 2109
RseI	CAYNNNNRTG	3 cut(s)	23, 107, 1481
SacI	GAGCTC	2 cut(s)	603, 2141
SaqAI	TTAA	7 cut(s)	21, 63, 636, 702, 1082, 2094, 2184
SatI	GCNGC	7 cut(s)	518, 1290, 1398, 1455, 1926, 2321, 2548
Sau96I	GGNCC	6 cut(s)	131, 132, 1036, 1246, 1523, 2173
SchI	GAGTC	7 cut(s)	68, 158, 1436, 1495, 1643, 2075, 2528
ScrFI	CCNGG	5 cut(s)	136, 282, 995, 1944, 2133
SduI	GDGCHC	6 cut(s)	135, 603, 993, 1856, 1942, 2141
SfaNI	GCATC	2 cut(s)	868, 932
SfcI	CTRYAG	2 cut(s)	168, 2545
SfuI	TTCGAA	2 cut(s)	440, 473
SinI	GGWCC	2 cut(s)	1036, 1523
SmiMI	CAYNNNNRTG	3 cut(s)	23, 107, 1481
SmlI	CTYRAG	2 cut(s)	1081, 1449
SmoI	CTYRAG	2 cut(s)	1081, 1449
SpeI	ACTAGT	1 cut(s)	2512
SphI	GCATGC	1 cut(s)	538
SsiI	CCGC	3 cut(s)	1205, 1264, 2321
SspI	AATATT	1 cut(s)	1260
SspMI	CTAG	5 cut(s)	327, 1236, 1473, 2492, 2513
SstI	GAGCTC	2 cut(s)	603, 2141
StyD4I	CCNGG	5 cut(s)	134, 280, 993, 1942, 2131
StyI	CCWWGG	1 cut(s)	1501
TaaI	ACNGT	7 cut(s)	267, 313, 373, 801, 895, 1010, 2459
TaiI	ACGT	4 cut(s)	463, 755, 2047, 2102
TaqI	TCGA	4 cut(s)	440, 473, 590, 2541
TatI	WGTACW	2 cut(s)	292, 1541
TauI	GCSGC	1 cut(s)	2323
Tru1I	TTAA	7 cut(s)	21, 63, 636, 702, 1082, 2094, 2184
Tru9I	TTAA	7 cut(s)	21, 63, 636, 702, 1082, 2094, 2184
TscAI	CASTG	8 cut(s)	114, 772, 1303, 1318, 1618, 2122, 2262, 2571
TseFI	GTSAC	2 cut(s)	205, 524
TseI	GCWGC	6 cut(s)	517, 1289, 1397, 1454, 1925, 2547
Tsp45I	GTSAC	2 cut(s)	205, 524
TspGWI	ACGGA	1 cut(s)	86
TspRI	CASTG	8 cut(s)	114, 772, 1303, 1318, 1618, 2122, 2262, 2571
Van91I	CCANNNNNTGG	1 cut(s)	733
Vha464I	CTTAAG	1 cut(s)	1081
VneI	GTGCAC	1 cut(s)	1938
VpaK11BI	GGWCC	2 cut(s)	1036, 1523
XagI	CCTNNNNNAGG	1 cut(s)	812
XapI	RAATTY	6 cut(s)	442, 619, 785, 962, 1384, 1657
XceI	RCATGY	2 cut(s)	538, 1753
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1795
XmiI	GTMKAC	1 cut(s)	72
XmnI	GAANNNNTTC	1 cut(s)	1375
XspI	CTAG	5 cut(s)	327, 1236, 1473, 2492, 2513
Zsp2I	ATGCAT	2 cut(s)	2278, 2331

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.