Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0006171

Physical Location & Seq

Genomic Coordinates Reverse (-)

6266691 .. 6279554

Gene Structure 12864 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031568

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4068 bp

ATGACTTTTGTAACGGTCACTTTGCTTCCTGGAGGCAGCAAATGGTTCCCAGGAAATATTCAGCTGTTTAAAGAAGCTCTTATGACCTTTGGCATTGAGAAGTTATCCGAGTTTCTCTGGAGAACTGATTATTTTGTTCTCATTTTGCAACTTAGGCATCTGAACGATGGATATGGTTCTTACCTATTGTCTAGCCTCGAGGCTAGCATTGGGAAAATTGATGACTTTCTCTCATTCGAGAGGGGATTTATATATGGGGACATAGTAAGCTCCGTAAGAGATCCATCTGGACAGATGGGAAAAGTTGTTGGCATTAAGATGTCTGTTGATTTGGAGAGTGTGAATGGAAATATGATCAAAAATGTAAACTCGAAGAATCTGTCAAAGATCCGTCCCATTTCGGTGGGGGATTATGTTGTTTGTGGGCCTTGGCTTGGAAGGGTGGATAGAGTAGTTGACTGTGTAGCTGTTGTCTTTGACGATGGCACAGAGTGTGTGGTGACTGCTGTGGATCCAGAGAAGCTCGTACCCGTCTCCCCAAACATACTTGAAGACCCACAGTACCCATATTATCCAGGACAGAGAGTAAAGGTTAGGCTCTCAACTGTTTCCAAATCAACTAGATGGTTTTGTGGTAACTGGAGGGAAAATCAAGATGAAGGAACTGTTTGTTCTTTGGAAGGAGGTTTAGTGTATGTTGATTGGCTTGCCTCTGTTCTCGTAGGTTGTGATTCGAATTTTCCTGCTCCTCCACATCTGCTGGATTCAAAGAAATTGACTTTGGTATCATGTTTCTCTCATGCCAATTGGCAGCTTGGTGACTGGTGTATGCTTGCAATTGCTGAGTGTAAAGGTCTTTCGGAGCTGGCTTTTCATAGAACTTCTACTTGTGAGCTCACTAACAAGCAAAACAATTCAGAAAGAGGATTTAAGAGAAGAAATCCCAAGTCAAAAATTGAGGAAATCTTTGTAATCTCAAAGACAAAGACCAAAGTTGATGTTCTGTGGCAAGATGGTAGCCAGTCTTTGGAAGTGGATTCACAGACATTAGTTCCGGTCAGTGTTGTAAATGATCATGAATTTTGGCCTGAACAGTTTGTCCTTGAAAAGGGAACTTATGATGATCCAAACTTGTTTAGCAGTCAAAGGTGGGGTGTTGTGCGTGGTGTGGATGCAAAGGAACGAACAGTAAAGGTGCAATGGAAAACTATAACTGCACCTGAAACAAATAATTTAGAGGGAGAGCAGATGGAGGAAGTTGTAAGTGCTTATGAACTTGTTGAGCACCCGGATTACTCCTACTGTTTTGGAGATTATGTGTTCAGGATGGTCCAGAATCAGTTTGATGAACAAGCTGATAAAAACTATCTGAACACAAGGTCTGACATGACTGAGGAGGTAGCTCCGGAAGATAAGAACTTCAGTGGGGATCAAGATGACTTCACAGATAAAAGTTACCTACCTTGCATTGGCAATGTTTTGGGCTTCATAGATGGTGCTGTAGAAGTGAGATGGGCAACTGGTATTACAAGCAAGGTGGCCCCATTTGAAATTTTCCAGTTTGATAAACATGAAGGTTCAGCTGCAATCCCCATAGAAGATGACACTGAGGAATCGAACAAAGAGATAATTCAACATGAAAAACAACCTAATAACGAAGAAGGAAAGGATTCCTTGAATTTAGATGATGCTGCTAAAAGTTGTAAACCGTACACAAGCAAGTCTAGTTCCTATTTCCTTCCTCAAGCTGCCATTGGATTTTTCTCGAGCATCGCTATGAGTCTCTTTGGATCCCATAGTTACACATCACTTTCAGGTCCTTCCTCATCAATCTGTACTTCTGAAGTTGGAAAGGAATCCGGGATCTCCCATGAGAAAGGAATAGTAGAAACTCGTGATGTCTGCACTGAGCCACATTCAACATCTGAGTCTGACATTATTGGCAATGAAAATTTGAATCATGAAGGCGAAGACATTGAGGGGAACAGATATCTTCCATGTTTGAAAGACAAAGAGACTGCAGATCAGTTCAGGCAGTTTGACATGCTTGCTGATTGTGCGGATCACCATTTTCATAGTGCCAGCAAGGAGTTGGTATTATCTCAGGTGAAAAGAGGTTGGATGAAGAAGGTTCAGCAAGAGTGGAGCATCTTAGAGAAAGATCTTCCTGAACAAATCTATGTCCGTGCCTTCGAGGGAAGGATGGATCTACTGCGGGCAGCCATTGTTGGTACACCAGGAACTCCATATCATGATGGGCTTTTCTTCTTTGACATTTATCTTCCACCAGAGTATCCTCATGAGCCACCTCTGGTACACTACATTTCTGGTGGGCTACGTGTGAACCCGAACTTGTATGAGTCTGGAAAGGTCTGTCTCAGTCTGCTTAACACGTGGACTGGTACGGGCAGTGAAGTTTGGAATCCTGGGAGCTCTACCATTCTTCAAGTTCTTCTCTCCCTTCAGGCCCTTGTGCTTAATGACAGGCCATATTTCAATGAAGCTGGATATGATCAGCAGGTGGGAAGAACTGAGGGAGAGAAAAACTCAGTGAGCTATAATGAAAATGCATTCCTTATGACTTGCAAGTGCATGCTGTTTTCTCTCCAAAAGCCGCCTAAGCATTTTGAGGGGCTTGTGATGGAACACTTCACTCAGCGCTCTGAAAATATTCTAATGGCTTGTAAGGCATATATGGAGGGGGTTCCAATAGGGTGTGCTGTTGATTTTCAGAAAACTGATGATAAAGAGGGAAGCTCTACTGGGTTCAAAATTATGCTTGCTAAGCTCTTTCAGAAACTAGTGGAGGCATTTTCTGCTAAGGGAATCGACTGCAACCACTCTCCCCTCTCCCCTCCACTGCACATCAATGGAAGTTCATCTCCATTCCACCAATCTAGGGTTAGGGTTCCCTTCCTCTTCCTCTTCTTCTTGTTCATCTTCATCACCATCACTACACCATCAACCCATGCAACTCCCTCCAATTTTCACTCCTTATACACCCAACACTGCAACGACGTCGTTCCAAACTCCCACCCCTTCCCCTCAAAACCGCTCCTCAACGACATCGGCACCGGATTCTTCACCGGCGGCGGCAGGCTTTTCACTCAAACCCACAACACAATCGCCTTGAATTTCAGGAAAGGCGTCAGCTTTCATGTCCAACATGGCGGCCCAATCGTCAGAGGGAGCTTGGCTCTTAAAGTTGAATCTGCTCCGGTGGTCTCACGGCTCTTTCGGCTCAATGGTTACTATTCAGATTCCGGTGACAAGCTCTGTATGGTTGGTACTGTTTCTCACATCAGTTCTGAGTCTCTGGTTGTTCTGAAGCTTAATTATCCCAAAAATACTAGTATTTTCGATATTTTAGTGACCGGGACTTTGGAGAGTGTTTCTGATGAGATAGATTTGAATTATTTTGAACCCATTTCGATACTGGGGTTATCTCGGAAATCTGGTTATCAGCCCACATTCATTGGGAAAGGTCGTCTGAATGGCTCTTTGGTTGGTTATGATAGAGGGGAGAGTTTAGGTATAGATAGTTTAGCTCTAGAGAAATTTGGTCGTTCGAGCCGCAGTGTATGTAGCGTTGTTGGCGGTTTTGGTTTTAGAGTAGAAAGATATGAATTGGAATATGAGAGTGGTAATGGCAATCCTCTTGGTGGAGATGTTGGATATGTACTTAAGTTCTTTGTTTTTCGTAGGACTCGGTGTGAAGATGGGAAGATGCAGATGTTGTTGGGGTTTCCCAACTCGAGTTTTCTTGGACGGGGTAGTACTTTTCCCTTTGAACCTAGGACAAGTTTGATTGCTGAAGGGGAATGGATTGAGAAGGAGAATCGTATGTTAGCTGTTGCGTGCAGGATTTTGAATTTTACGGAGTCATTGACTAATGCTGTTGTGGGAGATTGTTCGACTAGACTCAGCTTTAGGTTACCTGCACGGGTGTCTTTAAGAAATAGGTCTAATATTGTGGGGCAAATTTGGAGCAACAGAGCGGTGAATGACTCTGGTTACTTCGGTAAAATTGGGTTTCACAGAGTGTCGGAACAGTTGATGAAATTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006464 GO:0006807 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0010966 GO:0012505 GO:0015698 GO:0016036 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0030163 GO:0031625 GO:0031667 GO:0031668 GO:0031669 GO:0032446 GO:0032879 GO:0033554 GO:0034762 GO:0034765 GO:0036211 GO:0042592 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050789 GO:0050801 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0055062 GO:0055081 GO:0055083 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070647 GO:0071496 GO:0071704 GO:0072505 GO:0072506 GO:0098771 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903795 GO:1903959 GO:2000185

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

1356

Amino Acids

151.38

Weight (kDa)

5.48

Isoelectric Point (pI)

38.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tSH3-B_UBE2O	PF23046	135 - 267	1.6e-40	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	358 - 431	5.4e-18	UBE2O, SH3-B domain
UQ_con	PF00179	711 - 856	3.8e-22	Ubiquitin-conjugating enzyme
DUF2921_N	PF25333	1079 - 1144	3.2e-13	DUF2921 lipocalin-like domain
DUF2921_N	PF25333	1237 - 1343	6.7e-22	DUF2921 lipocalin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33770
fragaria_vesca	FvH4_3g09200 FvH4_3g29950
malus_domestica	MD05G1312700.v1.1 MD10G1294500.v1.1
prunus_persica	Prupe.4G047400_v2.0.a1 Prupe.4G047400_v2.0.a1
pyrus_communis	pycom05g29070 pycom05g29080 pycom10g24620 pycom10g24630 pycom10g24640
rosa_chinensis	RchiOBHm_Chr5g0008401
rosa_laevigata	RLG00000031568
rosa_multiflora	Rmu_co8007308.1_g000001 Rmu_co8292313.1_g000001 Rmu_sc0004326.1_g000003 Rmu_sc0028200.1_g000001 Rmu_ssc0000388.1_g000061
rosa_roxburghii	Rroxscaffold_1G00068090
rosa_rugosa	Rorug04G0435600
rosa_samantha	Rh5AG064200 Rh5BG061400 Rh5CG071300 Rh5DG060200 Rh5DG060300
rosa_wichuraiana	Rw5G005660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2509
AatII	GACGTC	1 cut(s)	3021
AbsI	CCTCGAGG	1 cut(s)	197
Acc36I	ACCTGC	2 cut(s)	2509, 3946
AccB1I	GGYRCC	1 cut(s)	3071
AccB7I	CCANNNNNTGG	1 cut(s)	1027
AccBSI	CCGCTC	2 cut(s)	3055, 3998
AccIII	TCCGGA	1 cut(s)	1405
AcuI	CTGAAG	5 cut(s)	1405, 1863, 2447, 3347, 3834
AcvI	CACGTG	1 cut(s)	2394
AcyI	GRCGYC	2 cut(s)	3018, 3147
AdeI	CACNNNGTG	2 cut(s)	492, 4042
AfeI	AGCGCT	1 cut(s)	2660
AfiI	CCNNNNNNNGG	6 cut(s)	434, 1027, 1108, 1469, 2899, 3662
AflII	CTTAAG	1 cut(s)	3683
AflIII	ACRYGT	2 cut(s)	2338, 2391
AhlI	ACTAGT	2 cut(s)	2800, 3350
AjnI	CCWGG	5 cut(s)	28, 49, 574, 2236, 2425
AjuI	GAANNNNNNNTTGG	4 cut(s)	764, 796, 3485, 3517
AloI	GAACNNNNNNTCC	2 cut(s)	1083, 1115
Alw21I	GWGCWC	3 cut(s)	897, 1287, 2435
Alw26I	GTCTC	6 cut(s)	538, 1787, 2009, 2381, 3229, 3318
Ama87I	CYCGRG	3 cut(s)	197, 1765, 3754
Aor13HI	TCCGGA	1 cut(s)	1405
Aor51HI	AGCGCT	1 cut(s)	2660
AoxI	GGCC	6 cut(s)	425, 1085, 1539, 2466, 2486, 3172
ApeKI	GCWGC	6 cut(s)	36, 811, 1583, 1691, 1748, 2219
ArsI	GACNNNNNNTTYG	4 cut(s)	365, 397, 3364, 3396
Asp700I	GAANNNNTTC	2 cut(s)	1669, 2670
AspA2I	CCTAGG	1 cut(s)	3794
AspLEI	GCGC	1 cut(s)	2661
AspS9I	GGNCC	6 cut(s)	425, 1330, 1540, 1817, 2467, 3173
AsuC2I	CCSGG	3 cut(s)	1289, 1861, 3376
AsuHPI	GGTGA	8 cut(s)	511, 830, 2057, 2119, 2938, 3076, 3278, 4012
AsuII	TTCGAA	1 cut(s)	734
AsuNHI	GCTAGC	1 cut(s)	203
AvaI	CYCGRG	3 cut(s)	197, 1765, 3754
AvaII	GGWCC	2 cut(s)	1330, 1817
AvrII	CCTAGG	1 cut(s)	3794
BaeI	ACNNNNGTAYC	6 cut(s)	768, 801, 2276, 2309, 3425, 3458
BamHI	GGATCC	2 cut(s)	511, 1790
BanI	GGYRCC	1 cut(s)	3071
BanII	GRGCYC	2 cut(s)	897, 2435
BauI	CACGAG	1 cut(s)	1893
BbrPI	CACGTG	1 cut(s)	2394
BbsI	GAAGAC	2 cut(s)	558, 1977
Bbv12I	GWGCWC	3 cut(s)	897, 1287, 2435
BbvI	GCAGC	6 cut(s)	48, 823, 1570, 1678, 1735, 2231
BceAI	ACGGC	1 cut(s)	3245
BcgI	CGANNNNNNTGC	2 cut(s)	3001, 3035
BciT130I	CCWGG	5 cut(s)	30, 51, 576, 2238, 2427
BciVI	GTATCC	1 cut(s)	2304
BclI	TGATCA	3 cut(s)	354, 1072, 2512
BcnI	CCSGG	3 cut(s)	1289, 1861, 3376
BcoDI	GTCTC	6 cut(s)	538, 1787, 2009, 2381, 3229, 3318
BcuI	ACTAGT	2 cut(s)	2800, 3350
BfmI	CTRYAG	2 cut(s)	1500, 2019
BfoI	RGCGCY	1 cut(s)	2662
BfrI	CTTAAG	1 cut(s)	3683
BfuAI	ACCTGC	2 cut(s)	2509, 3946
BfuI	GTATCC	1 cut(s)	2304
BglII	AGATCT	1 cut(s)	2161
BlnI	CCTAGG	1 cut(s)	3794
BlpI	GCTNAGC	1 cut(s)	2784
BmcAI	AGTACT	1 cut(s)	3778
Bme1390I	CCNGG	8 cut(s)	30, 51, 576, 1289, 1861, 2238, 2427, 3376
Bme18I	GGWCC	2 cut(s)	1330, 1817
BmeT110I	CYCGRG	3 cut(s)	197, 1765, 3754
BmgT120I	GGNCC	6 cut(s)	425, 1330, 1540, 1817, 2467, 3173
BmiI	GGNNCC	7 cut(s)	47, 513, 1542, 1792, 2706, 2910, 3073
BmrFI	CCNGG	8 cut(s)	30, 51, 576, 1289, 1861, 2238, 2427, 3376
BmrI	ACTGGG	2 cut(s)	2772, 3446
BmsI	GCATC	6 cut(s)	166, 1162, 1678, 1779, 2157, 3717
BmtI	GCTAGC	1 cut(s)	207
BmuI	ACTGGG	2 cut(s)	2772, 3446
BpiI	GAAGAC	2 cut(s)	558, 1977
BplI	GAGNNNNNCTC	6 cut(s)	2531, 2563, 2741, 2773, 3181, 3213
BpmI	CTGGAG	3 cut(s)	51, 139, 661
Bpu10I	CCTNAGC	2 cut(s)	2619, 2820
Bpu1102I	GCTNAGC	1 cut(s)	2784
Bpu14I	TTCGAA	1 cut(s)	734
BpuEI	CTTGAG	1 cut(s)	1728
BpuMI	CCSGG	3 cut(s)	1289, 1861, 3376
BsaAI	YACGTR	2 cut(s)	2339, 2394
BsaHI	GRCGYC	2 cut(s)	3018, 3147
BsaI	GGTCTC	1 cut(s)	3229
BsaJI	CCNNGG	4 cut(s)	49, 428, 2426, 3794
BsaWI	WCCGGW	5 cut(s)	1054, 1405, 3074, 3217, 3263
BsaXI	ACNNNNNCTCC	4 cut(s)	675, 705, 1302, 1332
Bsc4I	CCNNNNNNNGG	6 cut(s)	434, 1027, 1108, 1469, 2899, 3662
Bse118I	RCCGGY	1 cut(s)	3086
Bse1I	ACTGG	8 cut(s)	644, 827, 1021, 1525, 1558, 2404, 2767, 3441
Bse3DI	GCAATG	3 cut(s)	1205, 1480, 1951
BseAI	TCCGGA	1 cut(s)	1405
BseBI	CCWGG	5 cut(s)	30, 51, 576, 2238, 2427
BseDI	CCNNGG	4 cut(s)	49, 428, 2426, 3794
BseGI	GGATG	4 cut(s)	1177, 1332, 2127, 2208
BseLI	CCNNNNNNNGG	6 cut(s)	434, 1027, 1108, 1469, 2899, 3662
BseMI	GCAATG	3 cut(s)	1205, 1480, 1951
BseNI	ACTGG	8 cut(s)	644, 827, 1021, 1525, 1558, 2404, 2767, 3441
BseRI	GAGGAG	3 cut(s)	738, 1409, 3047
BseXI	GCAGC	6 cut(s)	48, 823, 1570, 1678, 1735, 2231
BsgI	GTGCAG	5 cut(s)	1200, 1888, 2846, 3880, 3924
BshFI	GGCC	6 cut(s)	427, 1087, 1541, 2468, 2488, 3174
BshNI	GGYRCC	1 cut(s)	3071
BsiHKAI	GWGCWC	3 cut(s)	897, 1287, 2435
BsiHKCI	CYCGRG	3 cut(s)	197, 1765, 3754
BsiSI	CCGG	9 cut(s)	1055, 1289, 1406, 1860, 3075, 3087, 3218, 3264, 3375
BslFI	GGGAC	3 cut(s)	272, 378, 3391
BslI	CCNNNNNNNGG	6 cut(s)	434, 1027, 1108, 1469, 2899, 3662
BsmAI	GTCTC	6 cut(s)	538, 1787, 2009, 2381, 3229, 3318
BsmBI	CGTCTC	1 cut(s)	538
BsmFI	GGGAC	3 cut(s)	272, 378, 3391
BsmI	GAATGC	1 cut(s)	2570
BsnI	GGCC	6 cut(s)	427, 1087, 1541, 2468, 2488, 3174
Bso31I	GGTCTC	1 cut(s)	3229
BsoBI	CYCGRG	3 cut(s)	197, 1765, 3754
Bsp119I	TTCGAA	1 cut(s)	734
Bsp1286I	GDGCHC	3 cut(s)	897, 1287, 2435
Bsp13I	TCCGGA	1 cut(s)	1405
Bsp1720I	GCTNAGC	1 cut(s)	2784
BspANI	GGCC	6 cut(s)	427, 1087, 1541, 2468, 2488, 3174
BspEI	TCCGGA	1 cut(s)	1405
BspHI	TCATGA	4 cut(s)	1075, 1960, 2251, 2299
BspLI	GGNNCC	7 cut(s)	47, 513, 1542, 1792, 2706, 2910, 3073
BspMAI	CTGCAG	1 cut(s)	2023
BspMI	ACCTGC	2 cut(s)	2509, 3946
BspOI	GCTAGC	1 cut(s)	207
BspT104I	TTCGAA	1 cut(s)	734
BspT107I	GGYRCC	1 cut(s)	3071
BspTI	CTTAAG	1 cut(s)	3683
BspTNI	GGTCTC	1 cut(s)	3229
BsrBI	CCGCTC	2 cut(s)	3055, 3998
BsrDI	GCAATG	3 cut(s)	1205, 1480, 1951
BsrFI	RCCGGY	1 cut(s)	3086
BsrI	ACTGG	8 cut(s)	644, 827, 1021, 1525, 1558, 2404, 2767, 3441
BssAI	RCCGGY	1 cut(s)	3086
BssECI	CCNNGG	4 cut(s)	49, 428, 2426, 3794
BssNI	GRCGYC	2 cut(s)	3018, 3147
BssSI	CACGAG	1 cut(s)	1893
BssT1I	CCWWGG	2 cut(s)	428, 3794
Bst2BI	CACGAG	1 cut(s)	1893
Bst2UI	CCWGG	5 cut(s)	30, 51, 576, 2238, 2427
Bst6I	CTCTTC	2 cut(s)	2924, 2930
BstACI	GRCGYC	2 cut(s)	3018, 3147
BstAFI	CTTAAG	1 cut(s)	3683
BstAPI	GCANNNNNTGC	1 cut(s)	2816
BstBAI	YACGTR	2 cut(s)	2339, 2394
BstBI	TTCGAA	1 cut(s)	734
BstEII	GGTNACC	1 cut(s)	3933
BstENI	CCTNNNNNAGG	1 cut(s)	1106
BstF5I	GGATG	4 cut(s)	1177, 1332, 2127, 2208
BstH2I	RGCGCY	1 cut(s)	2662
BstHHI	GCGC	1 cut(s)	2661
BstMAI	GTCTC	6 cut(s)	538, 1787, 2009, 2381, 3229, 3318
BstMWI	GCNNNNNNNGC	8 cut(s)	154, 2057, 2620, 2687, 2785, 2816, 3238, 3594
BstNI	CCWGG	5 cut(s)	30, 51, 576, 2238, 2427
BstNSI	RCATGY	2 cut(s)	2047, 2596
BstPI	GGTNACC	1 cut(s)	3933
BstSCI	CCNGG	8 cut(s)	28, 49, 574, 1287, 1859, 2236, 2425, 3374
BstSFI	CTRYAG	2 cut(s)	1500, 2019
BstV1I	GCAGC	6 cut(s)	48, 823, 1570, 1678, 1735, 2231
BstV2I	GAAGAC	2 cut(s)	558, 1977
BstX2I	RGATCY	7 cut(s)	280, 387, 511, 1790, 1863, 2161, 2206
BstXI	CCANNNNNNTGG	1 cut(s)	403
BstYI	RGATCY	7 cut(s)	280, 387, 511, 1790, 1863, 2161, 2206
BsuI	GTATCC	1 cut(s)	2304
BsuRI	GGCC	6 cut(s)	427, 1087, 1541, 2468, 2488, 3174
BtgZI	GCGATG	1 cut(s)	1756
BtsCI	GGATG	4 cut(s)	1177, 1332, 2127, 2208
BtsI	GCAGTG	4 cut(s)	2416, 2858, 3007, 3583
BtsIMutI	CAGTG	9 cut(s)	1066, 1429, 1605, 1905, 2416, 2556, 2858, 3007, 3583
BveI	ACCTGC	2 cut(s)	2509, 3946
CciI	TCATGA	4 cut(s)	1075, 1960, 2251, 2299
CfoI	GCGC	1 cut(s)	2661
Cfr10I	RCCGGY	1 cut(s)	3086
Cfr13I	GGNCC	6 cut(s)	425, 1330, 1540, 1817, 2467, 3173
CseI	GACGC	1 cut(s)	3136
CspCI	CAANNNNNGTGG	4 cut(s)	1518, 1553, 3457, 3492
DraI	TTTAAA	1 cut(s)	70
DraIII	CACNNNGTG	2 cut(s)	492, 4042
Eam1104I	CTCTTC	2 cut(s)	2924, 2930
EarI	CTCTTC	2 cut(s)	2924, 2930
Ecl136II	GAGCTC	2 cut(s)	895, 2433
Eco130I	CCWWGG	2 cut(s)	428, 3794
Eco24I	GRGCYC	2 cut(s)	897, 2435
Eco31I	GGTCTC	1 cut(s)	3229
Eco32I	GATATC	1 cut(s)	1991
Eco47I	GGWCC	2 cut(s)	1330, 1817
Eco47III	AGCGCT	1 cut(s)	2660
Eco53kI	GAGCTC	2 cut(s)	895, 2433
Eco57I	CTGAAG	5 cut(s)	1405, 1863, 2447, 3347, 3834
Eco72I	CACGTG	1 cut(s)	2394
Eco88I	CYCGRG	3 cut(s)	197, 1765, 3754
Eco91I	GGTNACC	1 cut(s)	3933
EcoICRI	GAGCTC	2 cut(s)	895, 2433
EcoNI	CCTNNNNNAGG	1 cut(s)	1106
EcoO109I	RGGNCCY	2 cut(s)	1817, 2467
EcoO65I	GGTNACC	1 cut(s)	3933
EcoRII	CCWGG	5 cut(s)	28, 49, 574, 2236, 2425
EcoRV	GATATC	1 cut(s)	1991
EcoT14I	CCWWGG	2 cut(s)	428, 3794
EcoT22I	ATGCAT	1 cut(s)	2572
EcoT38I	GRGCYC	2 cut(s)	897, 2435
ErhI	CCWWGG	2 cut(s)	428, 3794
Esp3I	CGTCTC	1 cut(s)	538
FalI	AAGNNNNNCTT	4 cut(s)	63, 95, 1658, 1690
FaqI	GGGAC	3 cut(s)	272, 378, 3391
FauI	CCCGC	1 cut(s)	2208
FbaI	TGATCA	3 cut(s)	354, 1072, 2512
FokI	GGATG	4 cut(s)	1184, 1339, 2134, 2215
FriOI	GRGCYC	2 cut(s)	897, 2435
GlaI	GCGC	1 cut(s)	2660
GsuI	CTGGAG	3 cut(s)	51, 139, 661
HaeII	RGCGCY	1 cut(s)	2662
HaeIII	GGCC	6 cut(s)	427, 1087, 1541, 2468, 2488, 3174
HapII	CCGG	9 cut(s)	1055, 1289, 1406, 1860, 3075, 3087, 3218, 3264, 3375
HgaI	GACGC	1 cut(s)	3136
HhaI	GCGC	1 cut(s)	2661
Hin1I	GRCGYC	2 cut(s)	3018, 3147
Hin6I	GCGC	1 cut(s)	2659
HinP1I	GCGC	1 cut(s)	2659
HincII	GTYRAC	1 cut(s)	457
HindII	GTYRAC	1 cut(s)	457
HindIII	AAGCTT	1 cut(s)	3329
HpaII	CCGG	9 cut(s)	1055, 1289, 1406, 1860, 3075, 3087, 3218, 3264, 3375
HphI	GGTGA	8 cut(s)	511, 830, 2057, 2119, 2938, 3076, 3278, 4012
Hpy166II	GTNNAC	8 cut(s)	367, 457, 1706, 1713, 2234, 2317, 2344, 2397
Hpy8I	GTNNAC	8 cut(s)	367, 457, 1706, 1713, 2234, 2317, 2344, 2397
Hpy99I	CGWCG	2 cut(s)	3020, 3023
HpyCH4IV	ACGT	3 cut(s)	2338, 2393, 3018
HpyF10VI	GCNNNNNNNGC	8 cut(s)	154, 2057, 2620, 2687, 2785, 2816, 3238, 3594
HpySE526I	ACGT	3 cut(s)	2338, 2393, 3018
Hsp92I	GRCGYC	2 cut(s)	3018, 3147
HspAI	GCGC	1 cut(s)	2659
Kpn2I	TCCGGA	1 cut(s)	1405
Ksp22I	TGATCA	3 cut(s)	354, 1072, 2512
Lsp1109I	GCAGC	6 cut(s)	48, 823, 1570, 1678, 1735, 2231
LweI	GCATC	6 cut(s)	166, 1162, 1678, 1779, 2157, 3717
MaeII	ACGT	3 cut(s)	2338, 2393, 3018
MbiI	CCGCTC	2 cut(s)	3055, 3998
MfeI	CAATTG	2 cut(s)	805, 837
MflI	RGATCY	7 cut(s)	280, 387, 511, 1790, 1863, 2161, 2206
MhlI	GDGCHC	3 cut(s)	897, 1287, 2435
MlyI	GAGTC	8 cut(s)	1789, 1937, 2369, 3320, 3700, 3890, 3915, 4001
MmeI	TCCRAC	5 cut(s)	1828, 2099, 3187, 3652, 4026
Mph1103I	ATGCAT	1 cut(s)	2572
MroI	TCCGGA	1 cut(s)	1405
MroXI	GAANNNNTTC	2 cut(s)	1669, 2670
MseI	TTAA	9 cut(s)	69, 315, 930, 2388, 2478, 3201, 3333, 3684, 3953
MslI	CAYNNNNRTG	4 cut(s)	317, 401, 1775, 2868
MspA1I	CMGCKG	2 cut(s)	64, 1583
MspCI	CTTAAG	1 cut(s)	3683
MspI	CCGG	9 cut(s)	1055, 1289, 1406, 1860, 3075, 3087, 3218, 3264, 3375
MspR9I	CCNGG	8 cut(s)	30, 51, 576, 1289, 1861, 2238, 2427, 3376
MunI	CAATTG	2 cut(s)	805, 837
Mva1269I	GAATGC	1 cut(s)	2570
MvaI	CCWGG	5 cut(s)	30, 51, 576, 2238, 2427
MwoI	GCNNNNNNNGC	8 cut(s)	154, 2057, 2620, 2687, 2785, 2816, 3238, 3594
NciI	CCSGG	3 cut(s)	1289, 1861, 3376
NheI	GCTAGC	1 cut(s)	203
NlaIV	GGNNCC	7 cut(s)	47, 513, 1542, 1792, 2706, 2910, 3073
NmuCI	GTSAC	5 cut(s)	16, 499, 818, 3266, 3370
NsiI	ATGCAT	1 cut(s)	2572
NspI	RCATGY	2 cut(s)	2047, 2596
NspV	TTCGAA	1 cut(s)	734
PaeI	GCATGC	1 cut(s)	2596
PaeR7I	CTCGAG	3 cut(s)	197, 1765, 3754
PagI	TCATGA	4 cut(s)	1075, 1960, 2251, 2299
PaqCI	CACCTGC	1 cut(s)	2509
PctI	GAATGC	1 cut(s)	2570
PdmI	GAANNNNTTC	2 cut(s)	1669, 2670
PflMI	CCANNNNNTGG	1 cut(s)	1027
PfoI	TCCNGGA	3 cut(s)	28, 574, 1859
PleI	GAGTC	8 cut(s)	1788, 1936, 2368, 3319, 3700, 3889, 3915, 4001
PmaCI	CACGTG	1 cut(s)	2394
PmlI	CACGTG	1 cut(s)	2394
PpsI	GAGTC	8 cut(s)	1788, 1936, 2368, 3319, 3700, 3889, 3915, 4001
Ppu21I	YACGTR	2 cut(s)	2339, 2394
PpuMI	RGGWCCY	1 cut(s)	1817
Psp124BI	GAGCTC	2 cut(s)	897, 2435
Psp5II	RGGWCCY	1 cut(s)	1817
Psp6I	CCWGG	5 cut(s)	28, 49, 574, 2236, 2425
PspCI	CACGTG	1 cut(s)	2394
PspEI	GGTNACC	1 cut(s)	3933
PspGI	CCWGG	5 cut(s)	28, 49, 574, 2236, 2425
PspN4I	GGNNCC	7 cut(s)	47, 513, 1542, 1792, 2706, 2910, 3073
PspPI	GGNCC	6 cut(s)	425, 1330, 1540, 1817, 2467, 3173
PspPPI	RGGWCCY	1 cut(s)	1817
PspXI	VCTCGAGB	2 cut(s)	197, 3754
PstI	CTGCAG	1 cut(s)	2023
PsuI	RGATCY	7 cut(s)	280, 387, 511, 1790, 1863, 2161, 2206
PvuII	CAGCTG	2 cut(s)	64, 1583
RseI	CAYNNNNRTG	4 cut(s)	317, 401, 1775, 2868
SacI	GAGCTC	2 cut(s)	897, 2435
SaqAI	TTAA	9 cut(s)	69, 315, 930, 2388, 2478, 3201, 3333, 3684, 3953
Sau96I	GGNCC	6 cut(s)	425, 1330, 1540, 1817, 2467, 3173
ScaI	AGTACT	1 cut(s)	3778
SchI	GAGTC	8 cut(s)	1789, 1937, 2369, 3320, 3700, 3890, 3915, 4001
ScrFI	CCNGG	8 cut(s)	30, 51, 576, 1289, 1861, 2238, 2427, 3376
SduI	GDGCHC	3 cut(s)	897, 1287, 2435
SfaNI	GCATC	6 cut(s)	166, 1162, 1678, 1779, 2157, 3717
SfcI	CTRYAG	2 cut(s)	1500, 2019
Sfr274I	CTCGAG	3 cut(s)	197, 1765, 3754
SfuI	TTCGAA	1 cut(s)	734
SgrAI	CRCCGGYG	1 cut(s)	3086
SinI	GGWCC	2 cut(s)	1330, 1817
SlaI	CTCGAG	3 cut(s)	197, 1765, 3754
SmiMI	CAYNNNNRTG	4 cut(s)	317, 401, 1775, 2868
SmlI	CTYRAG	5 cut(s)	197, 1743, 1765, 3683, 3754
SmoI	CTYRAG	5 cut(s)	197, 1743, 1765, 3683, 3754
SpeI	ACTAGT	2 cut(s)	2800, 3350
SphI	GCATGC	1 cut(s)	2596
SspI	AATATT	3 cut(s)	58, 2671, 3970
SstI	GAGCTC	2 cut(s)	897, 2435
StyD4I	CCNGG	8 cut(s)	28, 49, 574, 1287, 1859, 2236, 2425, 3374
StyI	CCWWGG	2 cut(s)	428, 3794
TaiI	ACGT	3 cut(s)	2341, 2396, 3021
TatI	WGTACW	3 cut(s)	1835, 3679, 3776
TauI	GCSGC	5 cut(s)	2617, 3093, 3096, 3174, 3576
Tru1I	TTAA	9 cut(s)	69, 315, 930, 2388, 2478, 3201, 3333, 3684, 3953
Tru9I	TTAA	9 cut(s)	69, 315, 930, 2388, 2478, 3201, 3333, 3684, 3953
TscAI	CASTG	9 cut(s)	1066, 1429, 1612, 1912, 2416, 2556, 2865, 3014, 3583
TseFI	GTSAC	5 cut(s)	16, 499, 818, 3266, 3370
TseI	GCWGC	6 cut(s)	36, 811, 1583, 1691, 1748, 2219
Tsp45I	GTSAC	5 cut(s)	16, 499, 818, 3266, 3370
TspGWI	ACGGA	4 cut(s)	262, 380, 2174, 3893
TspRI	CASTG	9 cut(s)	1066, 1429, 1612, 1912, 2416, 2556, 2865, 3014, 3583
Van91I	CCANNNNNTGG	1 cut(s)	1027
Vha464I	CTTAAG	1 cut(s)	3683
VpaK11BI	GGWCC	2 cut(s)	1330, 1817
XagI	CCTNNNNNAGG	1 cut(s)	1106
XbaI	TCTAGA	1 cut(s)	3550
XceI	RCATGY	2 cut(s)	2047, 2596
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2089, 3433
XhoI	CTCGAG	3 cut(s)	197, 1765, 3754
XmaJI	CCTAGG	1 cut(s)	3794
XmnI	GAANNNNTTC	2 cut(s)	1669, 2670
ZraI	GACGTC	1 cut(s)	3019
ZrmI	AGTACT	1 cut(s)	3778
Zsp2I	ATGCAT	1 cut(s)	2572

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.