Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0006171

Physical Location & Seq

Genomic Coordinates Forward (+)

5994536 .. 5997929

Gene Structure 3394 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G005660.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2709 bp

ATGGATATGGTTCTTAGTGACTCTGATTGGGACAGTTACAGTGAGACTGGTAGCAATGAGGATCAAGAGGGAAACGAATATTTCTATGGTGGGCATGCCAGCAGCCTATTGTCTAGCCTCGAGGCTAGCATTGGGAAAATTGATGACTTTCTCTCATTCGAGAGGGGATTTATATATGGGGACATAATGGGAAAAGTTGTTGGCATTAAGATGTCTGTTGATTTGGAGAGTGTGAATGGAAATATGATCAAAAATGTAAACTCAAAGAATCTGTCAAAGATCCGTCCCATTTCGGTGGGGGATTATGTTGTTTGTGGGCCTTGGCTTGGAAGGGTGGATAGAGTAGTTGACTGTGTAGCTGTTGTCTTTGACGATGGCACAGAGTGTGTGGTGACTGCTGTGGATCCAGAGAAGCTCGTACCCGTCTCCCCAAACATACTTGAAGACCCACAGTACCCATATTATCCAGGACAGAGAGTAAAGGTTAGGCTCTCAACTGTTTCCAAATCAACTAGATGGTTTTGTGGTAACTGGAGGGAAAATCAAGATGAAGGAACTGTTTGTTCTTTGGAAGGAGGTTTAATGTATGTTGATTGGCTTGCCTCTGTTCTCGTAGGTTGTGGTTCGAATTTTCCTGCTCCTCCACATCTGCTGGATTCAAAGAAATTGACTTTGGTATCATGTTTCTCTCATGCCAATTGGCAGCTTGGTGACTGGTGTATGCTTGCAATTGCTGAGTGTAAAGGTTTTTCGGAGCAGGCTTTTCATAGAACTTCTACTTGTGAGCTCACTAACAAGCAAAACAATTCAGAAAGAGGATTTAAGAGAAGAAATCCTAAGTCAAAAATTGAGGAAATCTTTGTAATCTCAAAGACAAAGACCAAAGTTGATGTTCTGTGGCAAGATGGTAGCCAGTCTTTGGAAGTGGATTCACAGACATTAGTTCCGGTCAGTGTTGTAAATGATCATGAATTTTGGCCTGAACAGTTTGTCCTTGAAAAGGGAACTTATGATGATCCAAACTTGTTTAGCAGCCAAAGGTGGGGTGTTGTGCGTGGTGTGGATGCAAAGGAACGAACAGTAAAGGTGCAATGGAAAACTATAACTGCACCCGAAACAAATAATTTAGAGGGAGAGCAGATGGAGGAAGTTGTAAGTGCTTATGAACTTGTTGAGCACCCGGATTACTCCTACTGTTTTGGAGATTATGTGTTCAGGATGGTCCAGAATCAGTTTGATGAACAAGCTGATAAAAACTATCCGAACACAAGGTCTGACATGACTGAGGAGGTAGCTCCGGAAGAGAAGAACTTCAGTGGGGATCAAGATGACTTCACAGATAAAAGTTACCTACCTTGCATTGGCAATGTTTTGGGCTTCATAGATGGTGCTTTAGAAGTGAGATGGGCAACTGGTATTACAAGCAAGGTGGCCCCATGTGAAATTTTCCGGTTTGATAAACATGAAGGTTCAGCTGCAATCCCTGTAGAAGATGACACTGAGGAATTGAACAAAGAGATAATTCAACATGAAAAACAACCTAATAACGAAAAAGGAAAGGATTCCTTGAATTTAGATGATGCTGCTAAAAGTTGTAAACCGTACACAAGCGAGTCTAGTTCCTATTTCCTTCCTCAAGCTGCCATTGGATTTTTCTCGAGCATCGCTGTGAGTCTCTTTGGATCCCATAGTTACACATCACTTTCAGGTCCTTCCCCATCAGTCTGTACTTCTGCAAAACTCGTGGTGTCTACTGAGCCACATTCAACGTCTGAGTCTGACATTATTGGCAATGAAAATTTGAATCATGAAGGCAAAGACATTGAGGGGAACAGATATCTTCCATGTTTGAAAGACAAAGAGAGTGCAGATCAGTTCAGGCAATTTGACATGCTTGCTGATTGTGCAGATCACCATTTTCATAGTGCCAGGAAGGAGTTGGCATTATCTCAGGTAAAAAGAGGTTGGATGAAGAAGGTTCAGCAAGAGTGGAGCATCTTAGAGAAAGATCTTCCTGAACAAATCTATGTCCGTGCGTTTGAGGAAAGGATGGATCTACTGCGGGCAGCCATTGTTGGTACACCAGGAACTCCATATCATGATGGGCTTTTCTTCTTTGACATTTATCTTCCACCAGAGTATCCTCATGATCCACCTATGGTACACTACACTTCTGGTGGGCTACGTGTGAACCCGAACTTGTATGAGTCTGGAAAGGTCTGTCTCAGTCTGCTTAACACGTGGACTGGTACGGGCAGTGAAGTTTGGAATCCTGGGAGCTCCACCATTCTTCAAGTTCTTCTCTCCCTTCAGGCCCTTGTGCTTAATGACAGGCCATATTTCAATGAAGCTGGATATGATCAGCAGGTGGGAAGAACTGAGGGAGAGAAAAACTCAGTGAGCTATAACGAAAATGCATTCCTTATGACTTGCAAGTCCATGCTGTTTTCTCTCCAAAAGCCGCCTAAGCATTTTGAGGGGCTTGTGATGGAACACTTCACTCAGCGCTCTGAAAATATTCTGATGTCTTGTAAGGCATATATGGAGGGGGTTCCAATAGGGTGTGCTGTTGATTTTCAGAAAACCGATGATAAAGAGGGAAGCTCTACTGGGTTCAAAATTATGCTTGCTAAGCTGTTTCAGAAACTAGTGGAGGCATTTTCTGCTAAGGGAATCGACTGCAGCCAGTTCATCGCGCCAGGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006464 GO:0006807 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0010966 GO:0012505 GO:0015698 GO:0016036 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0030163 GO:0031625 GO:0031667 GO:0031668 GO:0031669 GO:0032446 GO:0032879 GO:0033554 GO:0034762 GO:0034765 GO:0036211 GO:0042592 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050789 GO:0050801 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0055062 GO:0055081 GO:0055083 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070647 GO:0071496 GO:0071704 GO:0072505 GO:0072506 GO:0098771 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903795 GO:1903959 GO:2000185

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

902

Amino Acids

101.04

Weight (kDa)

4.86

Isoelectric Point (pI)

37.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tSH3-B_UBE2O	PF23046	99 - 231	3.7e-40	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	322 - 395	3.3e-18	UBE2O, SH3-B domain
UQ_con	PF00179	660 - 805	2.4e-23	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33770
fragaria_vesca	FvH4_3g09200 FvH4_3g29950
malus_domestica	MD05G1312700.v1.1 MD10G1294500.v1.1
prunus_persica	Prupe.4G047400_v2.0.a1 Prupe.4G047400_v2.0.a1
pyrus_communis	pycom05g29070 pycom05g29080 pycom10g24620 pycom10g24630 pycom10g24640
rosa_chinensis	RchiOBHm_Chr5g0008401
rosa_laevigata	RLG00000031568
rosa_multiflora	Rmu_co8007308.1_g000001 Rmu_co8292313.1_g000001 Rmu_sc0004326.1_g000003 Rmu_sc0028200.1_g000001 Rmu_ssc0000388.1_g000061
rosa_roxburghii	Rroxscaffold_1G00068090
rosa_rugosa	Rorug04G0435600
rosa_samantha	Rh5AG064200 Rh5BG061400 Rh5CG071300 Rh5DG060200 Rh5DG060300
rosa_wichuraiana	Rw5G005660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2356
AasI	GACNNNNNNGTC	1 cut(s)	2434
AbsI	CCTCGAGG	1 cut(s)	119
Acc36I	ACCTGC	1 cut(s)	2356
AccB7I	CCANNNNNTGG	1 cut(s)	919
AccI	GTMKAC	1 cut(s)	1751
AccII	CGCG	1 cut(s)	2696
AccIII	TCCGGA	1 cut(s)	1297
AciI	CCGC	2 cut(s)	2062, 2462
AcsI	RAATTY	5 cut(s)	628, 971, 1443, 1570, 1798
AcuI	CTGAAG	2 cut(s)	1297, 2294
AcvI	CACGTG	1 cut(s)	2241
AdeI	CACNNNGTG	1 cut(s)	384
AfaI	GTAC	7 cut(s)	420, 455, 1604, 1729, 2080, 2163, 2251
AfeI	AGCGCT	1 cut(s)	2507
AfiI	CCNNNNNNNGG	5 cut(s)	326, 919, 1000, 1042, 1361
AflIII	ACRYGT	2 cut(s)	2185, 2238
AhlI	ACTAGT	1 cut(s)	2647
AjnI	CCWGG	5 cut(s)	466, 1928, 2083, 2272, 2698
AjuI	GAANNNNNNNTTGG	2 cut(s)	656, 688
AloI	GAACNNNNNNTCC	2 cut(s)	975, 1007
Alw21I	GWGCWC	3 cut(s)	789, 1179, 2282
Alw26I	GTCTC	4 cut(s)	38, 430, 1679, 2228
Ama87I	CYCGRG	2 cut(s)	119, 1657
Aor13HI	TCCGGA	1 cut(s)	1297
Aor51HI	AGCGCT	1 cut(s)	2507
AoxI	GGCC	5 cut(s)	317, 977, 1431, 2313, 2333
ApeKI	GCWGC	8 cut(s)	102, 703, 1032, 1475, 1583, 1640, 2066, 2682
ApoI	RAATTY	5 cut(s)	628, 971, 1443, 1570, 1798
ArsI	GACNNNNNNTTYG	4 cut(s)	257, 289, 1256, 1288
Asp700I	GAANNNNTTC	3 cut(s)	1310, 1561, 2517
AspLEI	GCGC	2 cut(s)	2508, 2698
AspS9I	GGNCC	5 cut(s)	317, 1222, 1432, 1709, 2314
AsuC2I	CCSGG	1 cut(s)	1181
AsuHPI	GGTGA	3 cut(s)	403, 722, 1904
AsuII	TTCGAA	1 cut(s)	626
AsuNHI	GCTAGC	1 cut(s)	125
AvaI	CYCGRG	2 cut(s)	119, 1657
AvaII	GGWCC	2 cut(s)	1222, 1709
BaeI	ACNNNNGTAYC	4 cut(s)	660, 693, 2123, 2156
BamHI	GGATCC	2 cut(s)	403, 1682
BanII	GRGCYC	2 cut(s)	789, 2282
BauI	CACGAG	1 cut(s)	1742
BbrPI	CACGTG	1 cut(s)	2241
BbsI	GAAGAC	1 cut(s)	450
Bbv12I	GWGCWC	3 cut(s)	789, 1179, 2282
BbvI	GCAGC	8 cut(s)	114, 715, 1044, 1462, 1570, 1627, 2078, 2694
BciT130I	CCWGG	5 cut(s)	468, 1930, 2085, 2274, 2700
BciVI	GTATCC	1 cut(s)	2151
BclI	TGATCA	3 cut(s)	246, 964, 2359
BcnI	CCSGG	1 cut(s)	1181
BcoDI	GTCTC	4 cut(s)	38, 430, 1679, 2228
BcuI	ACTAGT	1 cut(s)	2647
BfaI	CTAG	5 cut(s)	114, 126, 513, 1617, 2648
BfmI	CTRYAG	2 cut(s)	1485, 2680
BfoI	RGCGCY	1 cut(s)	2509
BfuAI	ACCTGC	1 cut(s)	2356
BfuI	GTATCC	1 cut(s)	2151
BglII	AGATCT	1 cut(s)	2008
BisI	GCNGC	9 cut(s)	103, 704, 1033, 1476, 1584, 1641, 2067, 2462, 2683
BlpI	GCTNAGC	1 cut(s)	2631
BlsI	GCNGC	9 cut(s)	104, 705, 1034, 1477, 1585, 1642, 2068, 2463, 2684
Bme1390I	CCNGG	6 cut(s)	468, 1181, 1930, 2085, 2274, 2700
Bme18I	GGWCC	2 cut(s)	1222, 1709
BmeT110I	CYCGRG	2 cut(s)	119, 1657
BmgT120I	GGNCC	5 cut(s)	317, 1222, 1432, 1709, 2314
BmiI	GGNNCC	4 cut(s)	405, 1434, 1684, 2553
BmrFI	CCNGG	6 cut(s)	468, 1181, 1930, 2085, 2274, 2700
BmrI	ACTGGG	1 cut(s)	2619
BmsI	GCATC	4 cut(s)	1054, 1570, 1671, 2004
BmtI	GCTAGC	1 cut(s)	129
BmuI	ACTGGG	1 cut(s)	2619
BpiI	GAAGAC	1 cut(s)	450
BplI	GAGNNNNNCTC	4 cut(s)	2378, 2410, 2588, 2620
BpmI	CTGGAG	1 cut(s)	553
Bpu10I	CCTNAGC	2 cut(s)	2466, 2667
Bpu1102I	GCTNAGC	1 cut(s)	2631
Bpu14I	TTCGAA	1 cut(s)	626
BpuEI	CTTGAG	1 cut(s)	1620
BpuMI	CCSGG	1 cut(s)	1181
BsaAI	YACGTR	2 cut(s)	2186, 2241
BsaJI	CCNNGG	3 cut(s)	320, 2273, 2699
BsaWI	WCCGGW	3 cut(s)	946, 1297, 1449
BsaXI	ACNNNNNCTCC	2 cut(s)	1194, 1224
Bsc4I	CCNNNNNNNGG	5 cut(s)	326, 919, 1000, 1042, 1361
Bse1I	ACTGG	8 cut(s)	52, 536, 719, 913, 1417, 2251, 2614, 2686
Bse3DI	GCAATG	4 cut(s)	61, 1097, 1372, 1798
BseAI	TCCGGA	1 cut(s)	1297
BseBI	CCWGG	5 cut(s)	468, 1930, 2085, 2274, 2700
BseDI	CCNNGG	3 cut(s)	320, 2273, 2699
BseGI	GGATG	4 cut(s)	1069, 1224, 1974, 2055
BseLI	CCNNNNNNNGG	5 cut(s)	326, 919, 1000, 1042, 1361
BseMI	GCAATG	4 cut(s)	61, 1097, 1372, 1798
BseNI	ACTGG	8 cut(s)	52, 536, 719, 913, 1417, 2251, 2614, 2686
BseRI	GAGGAG	2 cut(s)	630, 1301
BseXI	GCAGC	8 cut(s)	114, 715, 1044, 1462, 1570, 1627, 2078, 2694
BsgI	GTGCAG	3 cut(s)	1092, 1887, 1926
Bsh1236I	CGCG	1 cut(s)	2696
BshFI	GGCC	5 cut(s)	319, 979, 1433, 2315, 2335
BsiHKAI	GWGCWC	3 cut(s)	789, 1179, 2282
BsiHKCI	CYCGRG	2 cut(s)	119, 1657
BsiSI	CCGG	4 cut(s)	947, 1181, 1298, 1450
BslFI	GGGAC	3 cut(s)	44, 194, 270
BslI	CCNNNNNNNGG	5 cut(s)	326, 919, 1000, 1042, 1361
BsmAI	GTCTC	4 cut(s)	38, 430, 1679, 2228
BsmBI	CGTCTC	1 cut(s)	430
BsmFI	GGGAC	3 cut(s)	44, 194, 270
BsmI	GAATGC	1 cut(s)	2417
BsnI	GGCC	5 cut(s)	319, 979, 1433, 2315, 2335
BsoBI	CYCGRG	2 cut(s)	119, 1657
Bsp119I	TTCGAA	1 cut(s)	626
Bsp1286I	GDGCHC	3 cut(s)	789, 1179, 2282
Bsp13I	TCCGGA	1 cut(s)	1297
Bsp1720I	GCTNAGC	1 cut(s)	2631
BspACI	CCGC	2 cut(s)	2062, 2462
BspANI	GGCC	5 cut(s)	319, 979, 1433, 2315, 2335
BspEI	TCCGGA	1 cut(s)	1297
BspFNI	CGCG	1 cut(s)	2696
BspHI	TCATGA	4 cut(s)	967, 1807, 2098, 2146
BspLI	GGNNCC	4 cut(s)	405, 1434, 1684, 2553
BspMAI	CTGCAG	1 cut(s)	2684
BspMI	ACCTGC	1 cut(s)	2356
BspOI	GCTAGC	1 cut(s)	129
BspT104I	TTCGAA	1 cut(s)	626
BsrDI	GCAATG	4 cut(s)	61, 1097, 1372, 1798
BsrI	ACTGG	8 cut(s)	52, 536, 719, 913, 1417, 2251, 2614, 2686
BssECI	CCNNGG	3 cut(s)	320, 2273, 2699
BssSI	CACGAG	1 cut(s)	1742
BssT1I	CCWWGG	1 cut(s)	320
Bst2BI	CACGAG	1 cut(s)	1742
Bst2UI	CCWGG	5 cut(s)	468, 1930, 2085, 2274, 2700
Bst6I	CTCTTC	1 cut(s)	1296
BstAPI	GCANNNNNTGC	1 cut(s)	2663
BstBAI	YACGTR	2 cut(s)	2186, 2241
BstBI	TTCGAA	1 cut(s)	626
BstC8I	GCNNGC	9 cut(s)	96, 100, 127, 600, 726, 759, 1896, 2064, 2628
BstENI	CCTNNNNNAGG	1 cut(s)	998
BstF5I	GGATG	4 cut(s)	1069, 1224, 1974, 2055
BstFNI	CGCG	1 cut(s)	2696
BstH2I	RGCGCY	1 cut(s)	2509
BstHHI	GCGC	2 cut(s)	2508, 2698
BstMAI	GTCTC	4 cut(s)	38, 430, 1679, 2228
BstMWI	GCNNNNNNNGC	4 cut(s)	1904, 2467, 2632, 2663
BstNI	CCWGG	5 cut(s)	468, 1930, 2085, 2274, 2700
BstNSI	RCATGY	2 cut(s)	98, 1894
BstSCI	CCNGG	6 cut(s)	466, 1179, 1928, 2083, 2272, 2698
BstSFI	CTRYAG	2 cut(s)	1485, 2680
BstUI	CGCG	1 cut(s)	2696
BstV1I	GCAGC	8 cut(s)	114, 715, 1044, 1462, 1570, 1627, 2078, 2694
BstV2I	GAAGAC	1 cut(s)	450
BstX2I	RGATCY	5 cut(s)	279, 403, 1682, 2008, 2053
BstXI	CCANNNNNNTGG	1 cut(s)	295
BstYI	RGATCY	5 cut(s)	279, 403, 1682, 2008, 2053
BsuI	GTATCC	1 cut(s)	2151
BsuRI	GGCC	5 cut(s)	319, 979, 1433, 2315, 2335
BtgZI	GCGATG	2 cut(s)	1648, 2677
BtsCI	GGATG	4 cut(s)	1069, 1224, 1974, 2055
BtsI	GCAGTG	1 cut(s)	2263
BtsIMutI	CAGTG	6 cut(s)	46, 958, 1321, 1497, 2263, 2403
BveI	ACCTGC	1 cut(s)	2356
Cac8I	GCNNGC	9 cut(s)	96, 100, 127, 600, 726, 759, 1896, 2064, 2628
CciI	TCATGA	4 cut(s)	967, 1807, 2098, 2146
CfoI	GCGC	2 cut(s)	2508, 2698
Cfr13I	GGNCC	5 cut(s)	317, 1222, 1432, 1709, 2314
Csp6I	GTAC	7 cut(s)	419, 454, 1603, 1728, 2079, 2162, 2250
CspCI	CAANNNNNGTGG	4 cut(s)	1410, 1445, 1725, 1760
CviQI	GTAC	7 cut(s)	419, 454, 1603, 1728, 2079, 2162, 2250
DraIII	CACNNNGTG	1 cut(s)	384
DrdI	GACNNNNNNGTC	1 cut(s)	2434
DseDI	GACNNNNNNGTC	1 cut(s)	2434
Eam1104I	CTCTTC	1 cut(s)	1296
EarI	CTCTTC	1 cut(s)	1296
Ecl136II	GAGCTC	2 cut(s)	787, 2280
Eco130I	CCWWGG	1 cut(s)	320
Eco24I	GRGCYC	2 cut(s)	789, 2282
Eco32I	GATATC	1 cut(s)	1838
Eco47I	GGWCC	2 cut(s)	1222, 1709
Eco47III	AGCGCT	1 cut(s)	2507
Eco53kI	GAGCTC	2 cut(s)	787, 2280
Eco57I	CTGAAG	2 cut(s)	1297, 2294
Eco72I	CACGTG	1 cut(s)	2241
Eco88I	CYCGRG	2 cut(s)	119, 1657
EcoICRI	GAGCTC	2 cut(s)	787, 2280
EcoNI	CCTNNNNNAGG	1 cut(s)	998
EcoO109I	RGGNCCY	2 cut(s)	1709, 2314
EcoRII	CCWGG	5 cut(s)	466, 1928, 2083, 2272, 2698
EcoRV	GATATC	1 cut(s)	1838
EcoT14I	CCWWGG	1 cut(s)	320
EcoT22I	ATGCAT	1 cut(s)	2419
EcoT38I	GRGCYC	2 cut(s)	789, 2282
ErhI	CCWWGG	1 cut(s)	320
Esp3I	CGTCTC	1 cut(s)	430
FalI	AAGNNNNNCTT	2 cut(s)	1550, 1582
FaqI	GGGAC	3 cut(s)	44, 194, 270
FauI	CCCGC	1 cut(s)	2055
FbaI	TGATCA	3 cut(s)	246, 964, 2359
FblI	GTMKAC	1 cut(s)	1751
Fnu4HI	GCNGC	9 cut(s)	103, 704, 1033, 1476, 1584, 1641, 2067, 2462, 2683
FokI	GGATG	4 cut(s)	1076, 1231, 1981, 2062
FriOI	GRGCYC	2 cut(s)	789, 2282
Fsp4HI	GCNGC	9 cut(s)	103, 704, 1033, 1476, 1584, 1641, 2067, 2462, 2683
FspBI	CTAG	5 cut(s)	114, 126, 513, 1617, 2648
GlaI	GCGC	2 cut(s)	2507, 2697
GluI	GCNGC	9 cut(s)	103, 704, 1033, 1476, 1584, 1641, 2067, 2462, 2683
GsuI	CTGGAG	1 cut(s)	553
HaeII	RGCGCY	1 cut(s)	2509
HaeIII	GGCC	5 cut(s)	319, 979, 1433, 2315, 2335
HapII	CCGG	4 cut(s)	947, 1181, 1298, 1450
HhaI	GCGC	2 cut(s)	2508, 2698
Hin6I	GCGC	2 cut(s)	2506, 2696
HinP1I	GCGC	2 cut(s)	2506, 2696
HincII	GTYRAC	1 cut(s)	349
HindII	GTYRAC	1 cut(s)	349
HpaII	CCGG	4 cut(s)	947, 1181, 1298, 1450
HphI	GGTGA	3 cut(s)	403, 722, 1904
Hpy166II	GTNNAC	9 cut(s)	259, 349, 1598, 1605, 1752, 2081, 2164, 2191, 2244
Hpy8I	GTNNAC	9 cut(s)	259, 349, 1598, 1605, 1752, 2081, 2164, 2191, 2244
HpyCH4IV	ACGT	3 cut(s)	1769, 2185, 2240
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1904, 2467, 2632, 2663
HpySE526I	ACGT	3 cut(s)	1769, 2185, 2240
HspAI	GCGC	2 cut(s)	2506, 2696
Kpn2I	TCCGGA	1 cut(s)	1297
Ksp22I	TGATCA	3 cut(s)	246, 964, 2359
LmnI	GCTCC	6 cut(s)	643, 754, 1300, 1992, 2277, 2285
Lsp1109I	GCAGC	8 cut(s)	114, 715, 1044, 1462, 1570, 1627, 2078, 2694
LweI	GCATC	4 cut(s)	1054, 1570, 1671, 2004
MaeI	CTAG	5 cut(s)	114, 126, 513, 1617, 2648
MaeII	ACGT	3 cut(s)	1769, 2185, 2240
MaeIII	GTNAC	7 cut(s)	17, 35, 391, 527, 710, 1346, 1691
MfeI	CAATTG	2 cut(s)	697, 729
MflI	RGATCY	5 cut(s)	279, 403, 1682, 2008, 2053
MhlI	GDGCHC	3 cut(s)	789, 1179, 2282
MlyI	GAGTC	5 cut(s)	14, 1622, 1681, 1784, 2216
MmeI	TCCRAC	1 cut(s)	1946
Mph1103I	ATGCAT	1 cut(s)	2419
MroI	TCCGGA	1 cut(s)	1297
MroXI	GAANNNNTTC	3 cut(s)	1310, 1561, 2517
MseI	TTAA	5 cut(s)	207, 581, 822, 2235, 2325
MslI	CAYNNNNRTG	3 cut(s)	209, 293, 1667
MspA1I	CMGCKG	1 cut(s)	1475
MspI	CCGG	4 cut(s)	947, 1181, 1298, 1450
MspR9I	CCNGG	6 cut(s)	468, 1181, 1930, 2085, 2274, 2700
MunI	CAATTG	2 cut(s)	697, 729
Mva1269I	GAATGC	1 cut(s)	2417
MvaI	CCWGG	5 cut(s)	468, 1930, 2085, 2274, 2700
MvnI	CGCG	1 cut(s)	2696
MwoI	GCNNNNNNNGC	4 cut(s)	1904, 2467, 2632, 2663
NciI	CCSGG	1 cut(s)	1181
NheI	GCTAGC	1 cut(s)	125
NlaIV	GGNNCC	4 cut(s)	405, 1434, 1684, 2553
NmuCI	GTSAC	3 cut(s)	17, 391, 710
NsiI	ATGCAT	1 cut(s)	2419
NspI	RCATGY	2 cut(s)	98, 1894
NspV	TTCGAA	1 cut(s)	626
PaeI	GCATGC	1 cut(s)	98
PaeR7I	CTCGAG	2 cut(s)	119, 1657
PagI	TCATGA	4 cut(s)	967, 1807, 2098, 2146
PaqCI	CACCTGC	1 cut(s)	2356
PctI	GAATGC	1 cut(s)	2417
PdmI	GAANNNNTTC	3 cut(s)	1310, 1561, 2517
PfeI	GAWTC	8 cut(s)	268, 656, 929, 1228, 1562, 1804, 2269, 2673
PflMI	CCANNNNNTGG	1 cut(s)	919
PfoI	TCCNGGA	1 cut(s)	466
PkrI	GCNGC	9 cut(s)	104, 705, 1034, 1477, 1585, 1642, 2068, 2463, 2684
PleI	GAGTC	5 cut(s)	14, 1621, 1680, 1783, 2215
PmaCI	CACGTG	1 cut(s)	2241
PmlI	CACGTG	1 cut(s)	2241
PpsI	GAGTC	5 cut(s)	14, 1621, 1680, 1783, 2215
Ppu21I	YACGTR	2 cut(s)	2186, 2241
PpuMI	RGGWCCY	1 cut(s)	1709
Psp124BI	GAGCTC	2 cut(s)	789, 2282
Psp5II	RGGWCCY	1 cut(s)	1709
Psp6I	CCWGG	5 cut(s)	466, 1928, 2083, 2272, 2698
PspCI	CACGTG	1 cut(s)	2241
PspGI	CCWGG	5 cut(s)	466, 1928, 2083, 2272, 2698
PspN4I	GGNNCC	4 cut(s)	405, 1434, 1684, 2553
PspPI	GGNCC	5 cut(s)	317, 1222, 1432, 1709, 2314
PspPPI	RGGWCCY	1 cut(s)	1709
PspXI	VCTCGAGB	1 cut(s)	119
PstI	CTGCAG	1 cut(s)	2684
PsuI	RGATCY	5 cut(s)	279, 403, 1682, 2008, 2053
PvuII	CAGCTG	1 cut(s)	1475
RsaI	GTAC	7 cut(s)	420, 455, 1604, 1729, 2080, 2163, 2251
RsaNI	GTAC	7 cut(s)	419, 454, 1603, 1728, 2079, 2162, 2250
RseI	CAYNNNNRTG	3 cut(s)	209, 293, 1667
SacI	GAGCTC	2 cut(s)	789, 2282
SaqAI	TTAA	5 cut(s)	207, 581, 822, 2235, 2325
SatI	GCNGC	9 cut(s)	103, 704, 1033, 1476, 1584, 1641, 2067, 2462, 2683
Sau96I	GGNCC	5 cut(s)	317, 1222, 1432, 1709, 2314
SchI	GAGTC	5 cut(s)	14, 1622, 1681, 1784, 2216
ScrFI	CCNGG	6 cut(s)	468, 1181, 1930, 2085, 2274, 2700
SduI	GDGCHC	3 cut(s)	789, 1179, 2282
SfaNI	GCATC	4 cut(s)	1054, 1570, 1671, 2004
SfcI	CTRYAG	2 cut(s)	1485, 2680
Sfr274I	CTCGAG	2 cut(s)	119, 1657
SfuI	TTCGAA	1 cut(s)	626
SinI	GGWCC	2 cut(s)	1222, 1709
SlaI	CTCGAG	2 cut(s)	119, 1657
SmiMI	CAYNNNNRTG	3 cut(s)	209, 293, 1667
SmlI	CTYRAG	3 cut(s)	119, 1635, 1657
SmoI	CTYRAG	3 cut(s)	119, 1635, 1657
SpeI	ACTAGT	1 cut(s)	2647
SphI	GCATGC	1 cut(s)	98
SsiI	CCGC	2 cut(s)	2062, 2462
SspI	AATATT	2 cut(s)	80, 2518
SspMI	CTAG	5 cut(s)	114, 126, 513, 1617, 2648
SstI	GAGCTC	2 cut(s)	789, 2282
StyD4I	CCNGG	6 cut(s)	466, 1179, 1928, 2083, 2272, 2698
StyI	CCWWGG	1 cut(s)	320
TaiI	ACGT	3 cut(s)	1772, 2188, 2243
TaqI	TCGA	5 cut(s)	120, 159, 626, 1658, 2676
TatI	WGTACW	1 cut(s)	1727
TauI	GCSGC	1 cut(s)	2464
TfiI	GAWTC	8 cut(s)	268, 656, 929, 1228, 1562, 1804, 2269, 2673
Tru1I	TTAA	5 cut(s)	207, 581, 822, 2235, 2325
Tru9I	TTAA	5 cut(s)	207, 581, 822, 2235, 2325
TscAI	CASTG	6 cut(s)	46, 958, 1321, 1504, 2263, 2403
TseFI	GTSAC	3 cut(s)	17, 391, 710
TseI	GCWGC	8 cut(s)	102, 703, 1032, 1475, 1583, 1640, 2066, 2682
Tsp45I	GTSAC	3 cut(s)	17, 391, 710
TspGWI	ACGGA	2 cut(s)	272, 2021
TspRI	CASTG	6 cut(s)	46, 958, 1321, 1504, 2263, 2403
Van91I	CCANNNNNTGG	1 cut(s)	919
VpaK11BI	GGWCC	2 cut(s)	1222, 1709
XagI	CCTNNNNNAGG	1 cut(s)	998
XapI	RAATTY	5 cut(s)	628, 971, 1443, 1570, 1798
XceI	RCATGY	2 cut(s)	98, 1894
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1936
XhoI	CTCGAG	2 cut(s)	119, 1657
XmiI	GTMKAC	1 cut(s)	1751
XmnI	GAANNNNTTC	3 cut(s)	1310, 1561, 2517
XspI	CTAG	5 cut(s)	114, 126, 513, 1617, 2648
Zsp2I	ATGCAT	1 cut(s)	2419

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.