Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0006171

Physical Location & Seq

Genomic Coordinates Reverse (-)

23069919 .. 23074468

Gene Structure 4550 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g29950.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2673 bp

ATGGACGTACTTCTTAGTGACTCTGATTGGGACAGTTACAGTGAGACTGATAGCAATGAGAATCAAGAGGGAAGCGACTATTTCTATGGTGGGCATGCCAGCAGCCTATTTTCTAGCCTTGAGGCAAGCATTGGGAAAATTGATGACTTCCTCTCATTCGAGAGCGGATTTATATATGGGGACAAAGTGTGCTCTGTAGGAGATCCATCTGGACAGATGGGAAAAGTTGTTGGCATTAAGATGTCTGTTGATTTGGAGACTGTGAATGGAAATGTAATCAAAGATGTAGACTCAAAGAATCTGTCAAAGATCCGTCCCATTTCAATGGGGGATTTTGTTGTTTGTGGGCCCTGGCTTGGAAGGGTGGACAGAGTGGTTGACTGTGTAGCTATCGTATTTGACGATGGCACAGATTGTATGGTGACTGTTGTGGATCCAGAGAAGCTCATACCCATCTCCCCAAACATACTTGAAGATCCACAGTACCCATATTATCCAGGACAAAGAGTACAGGTTATGCTTTCAACTGTTTCCAAATCAACTAGATGGTTATGCGGTAACTGGAGGGAAAATCAAGACGGGGGAACTGTTTGTTCTTTGCAAGGAGGTGTAGTGTATGTTGATTGGCTTGCCTCTATTCTCGTAGGTTGTGATTCGAATTTTCCTCCTCCTCCACGTCAGCTGGATTTGAAAAAATTGACTTTGGTGTCATGTTTCTCTCATGCCAATTGGCAGCTTGGTGACTGGTGCATGCTTGCAAATGCTGAGATTGAAGGTTTCTCAGAGCAGGCTTTTCATAGAACTCAGCTCACCAACAAGCAAGAAAATTCAGAAAGAGGATTTAGGAGAAGAAATCCCAAGTCAAAAATTGAGGAAATCTTTTTAATCTCAAAGACAAAAACCAAAGTTGATAATCTGTGGCAAGATGGCAGCCAGTCTTTGGAAGTGGATTCACAGACATTAGTTCCGGTCAGTGTTGTAAATGATCATGAATTTTGGCCTGAACAGTTTGTCCCTGAAAAGGGAACTTACGATGATCCAAACTTGCTCAGCAACCAACGGTGGGGTGTTGTGCGTGGTGTGGATGCAAAGGAACGAACAGTAAAGATGGGATGGAAAACTATTAGTGCTTCCGGAGCAAATAATTTAGAGGGAGAGCAGATGGAGGAAGTTGTAAGTGCTTATGAGCTTGTTGAGCTCCCGGATTACTCCTATTGTTTTGGAGATTATGTGTTCAGGATGGTCCAGAATCAGTTTGATGAACAACCTGATAAAATCTATCTGAGCACAAGGTTCAATATGACTGAGGAGGTGGCTCCGAAAGAGAAGAACTTTGGTGGGGATCAAGAAAGGTTCACAGATAACAGTTACCTACCTTGCATTGGCAATGTTTTGCGCTTCATAGATGGTGCGGTAGAAGTGGGATGGGCAACTGGTATTACTAGCAAGGTGGCCCCTCGTGAAATTTTCCGGTTTGATAAACGCGAAGGTTCAGCTGCAATCACTGCAGAAGAGGACACTGAGGAATTGAATATAGAGATAATTCCACATGAAAAACAAATTGATAACGAGAAAGGAAAGGATTCCTTGAATTTAGATAATGTTGCTAAAGGTTGCAAACAGCAAACTAGCGAGTCCAGTTCCTATTTCCTTCCTCAAGCTGCCATTGGATTTTTCTCAAGCATTACTGTGAGTCTCTTTGGATCTCATGGTTACACATCACTTTCAGGTCCTTCCCCATCAGTCTGTACTTCTGATGTTGGAAAGGAATCTGGGTTCTGCCATGAGAAAAGAATAGTAGAAACTCGTGATATCTGCACTGAGCCACATGCAATCTCTGAGTCTGATATTTCTGGCAATGAAAATTTGAATCATGAAGGCAAAGACATTGAGGGGAACATATATCTTCCATATTTGAAAGAAAAGGAGAGTGCTAATCAGTTCAGGCAGTTTGACATGCTTGCTGATTGTGCAGATCACCATTTTCATGGTGTCAGCAAGGAGTTGGCATTATCTCAGGTGAAAAAGGGTTGGATGAAGAAGGTTCAGCAAGAGTGGAGCATCTTAGTGAAAGATCGTCCTGAACAAATCTATGTCCGTGCCTTTGAGGAAAGGATGGATCTACTGAGGGCAGCCATCGTTGGTGCACCAGGGACTCCATATCATGATGGGCTTTTCTTCTTTGACATTTTTCTTCCACCAGGGTATCCTCATGACCCACCAACGGTACACTACATTTCTGGTGGGCTACGTGTGAACCCGAACTTGTATGAGTCTGGAAAGGCCCTTGTGCTTAACGACAGGCCATATTTCAATGAAGCTGGATATGATCAGCAGGTGGGAAGAACTGAGGGAGAGAAAAACTCAGTGAGCTACAATGAAAACGCATTCCTTATGACTTGCAAGACAATGCTGTATTCTCTCCAAAATCCCCCTATGCATTTCAAAGGACTTGTGATGGAAAACTTCACTCGGCGTTCTGAAAATATTCTATTGGCTTGTAAGGCATATATGAAGGGGGTTCCAATAGGGTGTGCTGTTGATTTTCAGAAAACCAATGATAAACACAGTAAGGGAAGCTCTACTGGGTTCAAGATTATGCTTGCTAAGCTCTTTCAGAAACTAGTGGAGTCATTTACTGCCAAAGGAATCGACTGCAGCCAGTTCATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006464 GO:0006807 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0010966 GO:0012505 GO:0015698 GO:0016036 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0030163 GO:0031625 GO:0031667 GO:0031668 GO:0031669 GO:0032446 GO:0032879 GO:0033554 GO:0034762 GO:0034765 GO:0036211 GO:0042592 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050789 GO:0050801 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0055062 GO:0055081 GO:0055083 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070647 GO:0071496 GO:0071704 GO:0072505 GO:0072506 GO:0098771 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903795 GO:1903959 GO:2000185

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

891

Amino Acids

99.76

Weight (kDa)

4.94

Isoelectric Point (pI)

40.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tSH3-B_UBE2O	PF23046	110 - 241	4.5e-38	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	329 - 402	9.2e-14	UBE2O, SH3-B domain
SH3-C_UBE2O	PF23044	452 - 495	8.8e-06	UBE2O, ubiquitin binding SH3-C domain
UQ_con	PF00179	682 - 761	1.8e-12	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33770
fragaria_vesca	FvH4_3g09200 FvH4_3g29950
malus_domestica	MD05G1312700.v1.1 MD10G1294500.v1.1
prunus_persica	Prupe.4G047400_v2.0.a1 Prupe.4G047400_v2.0.a1
pyrus_communis	pycom05g29070 pycom05g29080 pycom10g24620 pycom10g24630 pycom10g24640
rosa_chinensis	RchiOBHm_Chr5g0008401
rosa_laevigata	RLG00000031568
rosa_multiflora	Rmu_co8007308.1_g000001 Rmu_co8292313.1_g000001 Rmu_sc0004326.1_g000003 Rmu_sc0028200.1_g000001 Rmu_ssc0000388.1_g000061
rosa_roxburghii	Rroxscaffold_1G00068090
rosa_rugosa	Rorug04G0435600
rosa_samantha	Rh5AG064200 Rh5BG061400 Rh5CG071300 Rh5DG060200 Rh5DG060300
rosa_wichuraiana	Rw5G005660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2326
AasI	GACNNNNNNGTC	1 cut(s)	706
Acc36I	ACCTGC	1 cut(s)	2326
AccB7I	CCANNNNNTGG	1 cut(s)	940
AccBSI	CCGCTC	1 cut(s)	165
AccI	GTMKAC	1 cut(s)	288
AccII	CGCG	1 cut(s)	1485
AccIII	TCCGGA	1 cut(s)	1133
AciI	CCGC	3 cut(s)	165, 555, 1412
AclWI	GGATC	9 cut(s)	197, 304, 428, 441, 470, 1031, 1350, 1711, 2127
AcsI	RAATTY	6 cut(s)	658, 826, 992, 1464, 1591, 1864
AfaI	GTAC	5 cut(s)	9, 485, 510, 1750, 2229
AfiI	CCNNNNNNNGG	7 cut(s)	356, 940, 1021, 1022, 1063, 1382, 2224
AflIII	ACRYGT	1 cut(s)	2251
AhlI	ACTAGT	1 cut(s)	2623
AjiI	CACGTC	1 cut(s)	677
AjnI	CCWGG	4 cut(s)	350, 496, 2149, 2200
AloI	GAACNNNNNNTCC	4 cut(s)	996, 1028, 1760, 1792
Alw21I	GWGCWC	4 cut(s)	194, 1200, 1289, 2149
Alw26I	GTCTC	3 cut(s)	38, 251, 1700
Alw44I	GTGCAC	1 cut(s)	2145
AlwI	GGATC	9 cut(s)	197, 304, 428, 441, 470, 1031, 1350, 1711, 2127
Aor13HI	TCCGGA	1 cut(s)	1133
AoxI	GGCC	5 cut(s)	347, 998, 1452, 2283, 2303
ApaI	GGGCCC	1 cut(s)	351
ApaLI	GTGCAC	1 cut(s)	2145
ApeKI	GCWGC	7 cut(s)	102, 733, 930, 1496, 1661, 2132, 2658
ApoI	RAATTY	6 cut(s)	658, 826, 992, 1464, 1591, 1864
ArsI	GACNNNNNNTTYG	2 cut(s)	287, 319
Asp700I	GAANNNNTTC	2 cut(s)	1582, 2487
AspLEI	GCGC	1 cut(s)	1398
AspS9I	GGNCC	6 cut(s)	347, 348, 1243, 1453, 1730, 2284
AsuC2I	CCSGG	1 cut(s)	1202
AsuHPI	GGTGA	5 cut(s)	433, 752, 802, 1970, 2032
AsuII	TTCGAA	1 cut(s)	656
AvaII	GGWCC	2 cut(s)	1243, 1730
BaeGI	GKGCMC	2 cut(s)	351, 2149
BaeI	ACNNNNGTAYC	2 cut(s)	2189, 2222
BamHI	GGATCC	1 cut(s)	433
BanII	GRGCYC	2 cut(s)	351, 1200
BauI	CACGAG	2 cut(s)	1458, 1806
Bbv12I	GWGCWC	4 cut(s)	194, 1200, 1289, 2149
BbvI	GCAGC	6 cut(s)	114, 745, 942, 1483, 1648, 2144
BciT130I	CCWGG	4 cut(s)	352, 498, 2151, 2202
BciVI	GTATCC	1 cut(s)	2217
BclI	TGATCA	2 cut(s)	985, 2329
BcnI	CCSGG	1 cut(s)	1202
BcoDI	GTCTC	3 cut(s)	38, 251, 1700
BcuI	ACTAGT	1 cut(s)	2623
BfaI	CTAG	5 cut(s)	114, 543, 1443, 1629, 2624
BfmI	CTRYAG	3 cut(s)	195, 1506, 2656
BfuAI	ACCTGC	1 cut(s)	2326
BfuI	GTATCC	1 cut(s)	2217
BisI	GCNGC	7 cut(s)	103, 734, 931, 1497, 1662, 2133, 2659
BlpI	GCTNAGC	2 cut(s)	1049, 2607
BlsI	GCNGC	7 cut(s)	104, 735, 932, 1498, 1663, 2134, 2660
Bme1390I	CCNGG	5 cut(s)	352, 498, 1202, 2151, 2202
Bme18I	GGWCC	2 cut(s)	1243, 1730
BmgBI	CACGTC	1 cut(s)	677
BmgT120I	GGNCC	6 cut(s)	347, 348, 1243, 1453, 1730, 2284
BmiI	GGNNCC	5 cut(s)	349, 435, 1317, 1455, 2523
BmrFI	CCNGG	5 cut(s)	352, 498, 1202, 2151, 2202
BmrI	ACTGGG	1 cut(s)	2595
BmsI	GCATC	2 cut(s)	1075, 2070
BmuI	ACTGGG	1 cut(s)	2595
BplI	GAGNNNNNCTC	2 cut(s)	2348, 2380
BpmI	CTGGAG	1 cut(s)	583
Bpu1102I	GCTNAGC	2 cut(s)	1049, 2607
Bpu14I	TTCGAA	1 cut(s)	656
BpuEI	CTTGAG	3 cut(s)	140, 1641, 1663
BpuMI	CCSGG	1 cut(s)	1202
BsaAI	YACGTR	1 cut(s)	2252
BsaJI	CCNNGG	3 cut(s)	350, 2150, 2201
BsaWI	WCCGGW	3 cut(s)	967, 1133, 1470
BsaXI	ACNNNNNCTCC	4 cut(s)	597, 627, 1215, 1245
Bsc4I	CCNNNNNNNGG	7 cut(s)	356, 940, 1021, 1022, 1063, 1382, 2224
Bse1I	ACTGG	7 cut(s)	566, 749, 934, 1438, 1638, 2590, 2662
Bse3DI	GCAATG	3 cut(s)	61, 1393, 1864
BseAI	TCCGGA	1 cut(s)	1133
BseBI	CCWGG	4 cut(s)	352, 498, 2151, 2202
BseDI	CCNNGG	3 cut(s)	350, 2150, 2201
BseGI	GGATG	6 cut(s)	1090, 1118, 1245, 1430, 2040, 2121
BseLI	CCNNNNNNNGG	7 cut(s)	356, 940, 1021, 1022, 1063, 1382, 2224
BseMI	GCAATG	3 cut(s)	61, 1393, 1864
BseNI	ACTGG	7 cut(s)	566, 749, 934, 1438, 1638, 2590, 2662
BseRI	GAGGAG	3 cut(s)	657, 660, 1322
BseSI	GKGCMC	2 cut(s)	351, 2149
BseXI	GCAGC	6 cut(s)	114, 745, 942, 1483, 1648, 2144
BsgI	GTGCAG	2 cut(s)	1801, 1992
Bsh1236I	CGCG	1 cut(s)	1485
BshFI	GGCC	5 cut(s)	349, 1000, 1454, 2285, 2305
BsiHKAI	GWGCWC	4 cut(s)	194, 1200, 1289, 2149
BsiSI	CCGG	4 cut(s)	968, 1134, 1202, 1471
BslFI	GGGAC	5 cut(s)	44, 194, 300, 998, 2167
BslI	CCNNNNNNNGG	7 cut(s)	356, 940, 1021, 1022, 1063, 1382, 2224
BsmAI	GTCTC	3 cut(s)	38, 251, 1700
BsmFI	GGGAC	5 cut(s)	44, 194, 300, 998, 2167
BsmI	GAATGC	1 cut(s)	2387
BsnI	GGCC	5 cut(s)	349, 1000, 1454, 2285, 2305
Bsp119I	TTCGAA	1 cut(s)	656
Bsp120I	GGGCCC	1 cut(s)	347
Bsp1286I	GDGCHC	5 cut(s)	194, 351, 1200, 1289, 2149
Bsp13I	TCCGGA	1 cut(s)	1133
Bsp1720I	GCTNAGC	2 cut(s)	1049, 2607
BspACI	CCGC	3 cut(s)	165, 555, 1412
BspANI	GGCC	5 cut(s)	349, 1000, 1454, 2285, 2305
BspEI	TCCGGA	1 cut(s)	1133
BspFNI	CGCG	1 cut(s)	1485
BspHI	TCATGA	4 cut(s)	988, 1873, 2164, 2212
BspLI	GGNNCC	5 cut(s)	349, 435, 1317, 1455, 2523
BspMAI	CTGCAG	2 cut(s)	1510, 2660
BspMI	ACCTGC	1 cut(s)	2326
BspPI	GGATC	9 cut(s)	197, 304, 428, 441, 470, 1031, 1350, 1711, 2127
BspT104I	TTCGAA	1 cut(s)	656
BsrBI	CCGCTC	1 cut(s)	165
BsrDI	GCAATG	3 cut(s)	61, 1393, 1864
BsrI	ACTGG	7 cut(s)	566, 749, 934, 1438, 1638, 2590, 2662
BssECI	CCNNGG	3 cut(s)	350, 2150, 2201
BssSI	CACGAG	2 cut(s)	1458, 1806
Bst2BI	CACGAG	2 cut(s)	1458, 1806
Bst2UI	CCWGG	4 cut(s)	352, 498, 2151, 2202
Bst6I	CTCTTC	1 cut(s)	1506
BstAPI	GCANNNNNTGC	1 cut(s)	1505
BstBAI	YACGTR	1 cut(s)	2252
BstBI	TTCGAA	1 cut(s)	656
BstC8I	GCNNGC	9 cut(s)	96, 100, 127, 630, 752, 756, 789, 1962, 2604
BstF5I	GGATG	6 cut(s)	1090, 1118, 1245, 1430, 2040, 2121
BstFNI	CGCG	1 cut(s)	1485
BstHHI	GCGC	1 cut(s)	1398
BstMAI	GTCTC	3 cut(s)	38, 251, 1700
BstMWI	GCNNNNNNNGC	6 cut(s)	1136, 1195, 1505, 1970, 2504, 2608
BstNI	CCWGG	4 cut(s)	352, 498, 2151, 2202
BstNSI	RCATGY	4 cut(s)	98, 754, 1832, 1960
BstSCI	CCNGG	5 cut(s)	350, 496, 1200, 2149, 2200
BstSFI	CTRYAG	3 cut(s)	195, 1506, 2656
BstSLI	GKGCMC	2 cut(s)	351, 2149
BstUI	CGCG	1 cut(s)	1485
BstV1I	GCAGC	6 cut(s)	114, 745, 942, 1483, 1648, 2144
BstX2I	RGATCY	6 cut(s)	202, 309, 433, 475, 1703, 2119
BstXI	CCANNNNNNTGG	2 cut(s)	325, 1988
BstYI	RGATCY	6 cut(s)	202, 309, 433, 475, 1703, 2119
BsuI	GTATCC	1 cut(s)	2217
BsuRI	GGCC	5 cut(s)	349, 1000, 1454, 2285, 2305
BtrI	CACGTC	1 cut(s)	677
BtsCI	GGATG	6 cut(s)	1090, 1118, 1245, 1430, 2040, 2121
BtsI	GCAGTG	1 cut(s)	1503
BtsIMutI	CAGTG	6 cut(s)	46, 979, 1503, 1518, 1818, 2373
BveI	ACCTGC	1 cut(s)	2326
Cac8I	GCNNGC	9 cut(s)	96, 100, 127, 630, 752, 756, 789, 1962, 2604
CciI	TCATGA	4 cut(s)	988, 1873, 2164, 2212
CfoI	GCGC	1 cut(s)	1398
Cfr13I	GGNCC	6 cut(s)	347, 348, 1243, 1453, 1730, 2284
Csp6I	GTAC	5 cut(s)	8, 484, 509, 1749, 2228
CviQI	GTAC	5 cut(s)	8, 484, 509, 1749, 2228
DrdI	GACNNNNNNGTC	1 cut(s)	706
DseDI	GACNNNNNNGTC	1 cut(s)	706
Eam1104I	CTCTTC	1 cut(s)	1506
EarI	CTCTTC	1 cut(s)	1506
Ecl136II	GAGCTC	1 cut(s)	1198
Eco24I	GRGCYC	2 cut(s)	351, 1200
Eco32I	GATATC	1 cut(s)	1813
Eco47I	GGWCC	2 cut(s)	1243, 1730
Eco53kI	GAGCTC	1 cut(s)	1198
EcoICRI	GAGCTC	1 cut(s)	1198
EcoO109I	RGGNCCY	3 cut(s)	348, 1730, 2284
EcoRII	CCWGG	4 cut(s)	350, 496, 2149, 2200
EcoRV	GATATC	1 cut(s)	1813
EcoT22I	ATGCAT	1 cut(s)	2442
EcoT38I	GRGCYC	2 cut(s)	351, 1200
FalI	AAGNNNNNCTT	2 cut(s)	1571, 1603
FaqI	GGGAC	5 cut(s)	44, 194, 300, 998, 2167
FbaI	TGATCA	2 cut(s)	985, 2329
FblI	GTMKAC	1 cut(s)	288
Fnu4HI	GCNGC	7 cut(s)	103, 734, 931, 1497, 1662, 2133, 2659
FokI	GGATG	6 cut(s)	1097, 1125, 1252, 1437, 2047, 2128
FriOI	GRGCYC	2 cut(s)	351, 1200
Fsp4HI	GCNGC	7 cut(s)	103, 734, 931, 1497, 1662, 2133, 2659
FspBI	CTAG	5 cut(s)	114, 543, 1443, 1629, 2624
GlaI	GCGC	1 cut(s)	1397
GluI	GCNGC	7 cut(s)	103, 734, 931, 1497, 1662, 2133, 2659
GsuI	CTGGAG	1 cut(s)	583
HaeIII	GGCC	5 cut(s)	349, 1000, 1454, 2285, 2305
HapII	CCGG	4 cut(s)	968, 1134, 1202, 1471
HhaI	GCGC	1 cut(s)	1398
Hin6I	GCGC	1 cut(s)	1396
HinP1I	GCGC	1 cut(s)	1396
HincII	GTYRAC	1 cut(s)	379
HindII	GTYRAC	1 cut(s)	379
HpaII	CCGG	4 cut(s)	968, 1134, 1202, 1471
HphI	GGTGA	5 cut(s)	433, 752, 802, 1970, 2032
Hpy166II	GTNNAC	7 cut(s)	289, 367, 379, 1356, 2147, 2230, 2257
Hpy8I	GTNNAC	7 cut(s)	289, 367, 379, 1356, 2147, 2230, 2257
HpyAV	CCTTC	8 cut(s)	354, 767, 1481, 1661, 1743, 1871, 2035, 2509
HpyCH4IV	ACGT	3 cut(s)	6, 676, 2251
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1136, 1195, 1505, 1970, 2504, 2608
HpySE526I	ACGT	3 cut(s)	6, 676, 2251
HspAI	GCGC	1 cut(s)	1396
Kpn2I	TCCGGA	1 cut(s)	1133
Ksp22I	TGATCA	2 cut(s)	985, 2329
LmnI	GCTCC	4 cut(s)	1136, 1203, 1321, 2058
Lsp1109I	GCAGC	6 cut(s)	114, 745, 942, 1483, 1648, 2144
LweI	GCATC	2 cut(s)	1075, 2070
MaeI	CTAG	5 cut(s)	114, 543, 1443, 1629, 2624
MaeII	ACGT	3 cut(s)	6, 676, 2251
MaeIII	GTNAC	7 cut(s)	17, 35, 421, 557, 740, 1367, 1712
MbiI	CCGCTC	1 cut(s)	165
MboII	GAAGA	9 cut(s)	485, 861, 1339, 1523, 1898, 2050, 2170, 2186, 2355
MfeI	CAATTG	1 cut(s)	727
MflI	RGATCY	6 cut(s)	202, 309, 433, 475, 1703, 2119
MhlI	GDGCHC	5 cut(s)	194, 351, 1200, 1289, 2149
MlyI	GAGTC	8 cut(s)	14, 284, 1643, 1702, 1850, 2149, 2282, 2639
MmeI	TCCRAC	2 cut(s)	1741, 2012
Mph1103I	ATGCAT	1 cut(s)	2442
MroI	TCCGGA	1 cut(s)	1133
MroXI	GAANNNNTTC	2 cut(s)	1582, 2487
MseI	TTAA	3 cut(s)	237, 884, 2295
MslI	CAYNNNNRTG	5 cut(s)	239, 323, 1688, 1986, 2066
MspA1I	CMGCKG	2 cut(s)	682, 1496
MspI	CCGG	4 cut(s)	968, 1134, 1202, 1471
MspR9I	CCNGG	5 cut(s)	352, 498, 1202, 2151, 2202
MunI	CAATTG	1 cut(s)	727
Mva1269I	GAATGC	1 cut(s)	2387
MvaI	CCWGG	4 cut(s)	352, 498, 2151, 2202
MvnI	CGCG	1 cut(s)	1485
MwoI	GCNNNNNNNGC	6 cut(s)	1136, 1195, 1505, 1970, 2504, 2608
NciI	CCSGG	1 cut(s)	1202
NlaIV	GGNNCC	5 cut(s)	349, 435, 1317, 1455, 2523
NmeAIII	GCCGAG	1 cut(s)	2452
NmuCI	GTSAC	3 cut(s)	17, 421, 740
NsiI	ATGCAT	1 cut(s)	2442
NspI	RCATGY	4 cut(s)	98, 754, 1832, 1960
NspV	TTCGAA	1 cut(s)	656
PaeI	GCATGC	2 cut(s)	98, 754
PagI	TCATGA	4 cut(s)	988, 1873, 2164, 2212
PaqCI	CACCTGC	1 cut(s)	2326
PcsI	WCGNNNNNNNCGW	1 cut(s)	399
PctI	GAATGC	1 cut(s)	2387
PdmI	GAANNNNTTC	2 cut(s)	1582, 2487
PfeI	GAWTC	9 cut(s)	61, 298, 653, 950, 1249, 1583, 1769, 1870, 2649
PflMI	CCANNNNNTGG	1 cut(s)	940
PfoI	TCCNGGA	2 cut(s)	496, 1200
PkrI	GCNGC	7 cut(s)	104, 735, 932, 1498, 1663, 2134, 2660
PleI	GAGTC	8 cut(s)	14, 284, 1642, 1701, 1849, 2149, 2281, 2638
PpsI	GAGTC	8 cut(s)	14, 284, 1642, 1701, 1849, 2149, 2281, 2638
Ppu21I	YACGTR	1 cut(s)	2252
PpuMI	RGGWCCY	1 cut(s)	1730
Psp124BI	GAGCTC	1 cut(s)	1200
Psp5II	RGGWCCY	1 cut(s)	1730
Psp6I	CCWGG	4 cut(s)	350, 496, 2149, 2200
PspGI	CCWGG	4 cut(s)	350, 496, 2149, 2200
PspN4I	GGNNCC	5 cut(s)	349, 435, 1317, 1455, 2523
PspOMI	GGGCCC	1 cut(s)	347
PspPI	GGNCC	6 cut(s)	347, 348, 1243, 1453, 1730, 2284
PspPPI	RGGWCCY	1 cut(s)	1730
PstI	CTGCAG	2 cut(s)	1510, 2660
PsuI	RGATCY	6 cut(s)	202, 309, 433, 475, 1703, 2119
PvuII	CAGCTG	2 cut(s)	682, 1496
RsaI	GTAC	5 cut(s)	9, 485, 510, 1750, 2229
RsaNI	GTAC	5 cut(s)	8, 484, 509, 1749, 2228
RseI	CAYNNNNRTG	5 cut(s)	239, 323, 1688, 1986, 2066
SacI	GAGCTC	1 cut(s)	1200
SaqAI	TTAA	3 cut(s)	237, 884, 2295
SatI	GCNGC	7 cut(s)	103, 734, 931, 1497, 1662, 2133, 2659
Sau96I	GGNCC	6 cut(s)	347, 348, 1243, 1453, 1730, 2284
SchI	GAGTC	8 cut(s)	14, 284, 1643, 1702, 1850, 2149, 2282, 2639
ScrFI	CCNGG	5 cut(s)	352, 498, 1202, 2151, 2202
SduI	GDGCHC	5 cut(s)	194, 351, 1200, 1289, 2149
SfaNI	GCATC	2 cut(s)	1075, 2070
SfcI	CTRYAG	3 cut(s)	195, 1506, 2656
SfuI	TTCGAA	1 cut(s)	656
SinI	GGWCC	2 cut(s)	1243, 1730
SmiMI	CAYNNNNRTG	5 cut(s)	239, 323, 1688, 1986, 2066
SmlI	CTYRAG	3 cut(s)	119, 1656, 1678
SmoI	CTYRAG	3 cut(s)	119, 1656, 1678
SpeI	ACTAGT	1 cut(s)	2623
SphI	GCATGC	2 cut(s)	98, 754
SsiI	CCGC	3 cut(s)	165, 555, 1412
SspI	AATATT	1 cut(s)	2488
SspMI	CTAG	5 cut(s)	114, 543, 1443, 1629, 2624
SstI	GAGCTC	1 cut(s)	1200
StyD4I	CCNGG	5 cut(s)	350, 496, 1200, 2149, 2200
TaiI	ACGT	3 cut(s)	9, 679, 2254
TaqI	TCGA	3 cut(s)	159, 656, 2652
TatI	WGTACW	2 cut(s)	508, 1748
TfiI	GAWTC	9 cut(s)	61, 298, 653, 950, 1249, 1583, 1769, 1870, 2649
Tru1I	TTAA	3 cut(s)	237, 884, 2295
Tru9I	TTAA	3 cut(s)	237, 884, 2295
TscAI	CASTG	6 cut(s)	46, 979, 1510, 1525, 1825, 2373
TseFI	GTSAC	3 cut(s)	17, 421, 740
TseI	GCWGC	7 cut(s)	102, 733, 930, 1496, 1661, 2132, 2658
Tsp45I	GTSAC	3 cut(s)	17, 421, 740
TspGWI	ACGGA	2 cut(s)	302, 2087
TspRI	CASTG	6 cut(s)	46, 979, 1510, 1525, 1825, 2373
Van91I	CCANNNNNTGG	1 cut(s)	940
VneI	GTGCAC	1 cut(s)	2145
VpaK11BI	GGWCC	2 cut(s)	1243, 1730
XapI	RAATTY	6 cut(s)	658, 826, 992, 1464, 1591, 1864
XceI	RCATGY	4 cut(s)	98, 754, 1832, 1960
XmiI	GTMKAC	1 cut(s)	288
XmnI	GAANNNNTTC	2 cut(s)	1582, 2487
XspI	CTAG	5 cut(s)	114, 543, 1443, 1629, 2624
Zsp2I	ATGCAT	1 cut(s)	2442

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.