Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006171

Physical Location & Seq

Genomic Coordinates Forward (+)

44336704 .. 44343243

Gene Structure 6540 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1312700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2691 bp

ATGGATCAAGAGGAAAACGAATATTTCTATGGTGGGCATGCCAGTAACATATTGTCCAGCCTAGAGGCGTCCATTGGGAAAATCGATGATTTTCTCACGTTTGAGAGGGGGTTCGTATATGGGGACATGGTATCCTCTGTAAGAGATCCATCTGGACAGATGGGCAGAGTGGTTGGGATTAACATGTTGGTCGATTTGGAGAGTGATAATGGAAATATAATAAAAGATGTAAACTCCAAAAACCTATCAAAGATCCGTTCCATTTCGGTGGGGGACTATGTTGTTTCTGGCCCATGGCTTGGAAGGGTGGATAGAGTGGTTGACCGTGTTACTGTTGTCTTTGATGATGGAACGGAGCGTGAGGTCACTGCTGTGGATCAAGAGAAACTCTTACCTATTTCTCCAAGCCTACTTGAAGATCCTCAGTACCCATATTATCCAGGCCAGAGAGTGCAGGTCAGGCTCTCAACTGCTTCCAAATCAAGTAGATGGTTATGCGGTAACTGGAGGGAAAATCAAGATGAGGGAACTGTTTGTTCTGTGGAAGGAGGTTTAGTGTATGTTGACTGGCTTGCCTCTTTTCTCATGGGTTGCGATCAGAAAATGCCTGCTCCTCCACGTGTGCTGGATTCGAAAAAATTGAATTTGTTGTCATGTTTCTCTCATGCAAATTGGCAGCTTGGTGACTGGTGTACGCTTCAAGTTGCTGATGATAAGGCTGTCATGGAGCAGGCTTTTCAAAATGCTTCTACTTGTGAGATGGTTAAGAAGCACAAGAAATCAGAAAGAGGATTTCGGAGAAGTAACATGAATTCAAGGGATGAAGTATTCGTGATCATAAAGACAAGGACCAAAGTTGATGTGGCGTGGCAAGATGGTAGCCACTCTTTGGGATTAGATTCACAGACACTAGTTCCAGTAAGCGTTGTAAATGATCATGAATTTTGGCCTGAACAGTTTGTCCTGGAAAAAGGAACTTACGATGATCTGCACATGTCTAGCAACCAAAAATGGGGTGTCGTGAGGGGTGTGGATGCAAAGGAACATACAGTAAAGGTGCAATGGAAAACCATCACTGCACCTGAAGAAAAGTACTCCAAGGGAGAGCAGTTGGAGGAAACCGTAAGCGCTTATGAACTGGTTAAGCACCCAGATTACTCCTACTGTTTTGGCGATTATGTGTTCAGGTTGGTCCAGAATCAGTTTGATGAACAAGCGGATAAAAACTGTCCACACACAAAGATTGACATGAGCAAGGAGGCTGCTTCTGACGACAAGAACTGTGGTGGAGATCAAGAGTATTACACTGATAAACGCTACCTATCTCACATTGGAAATGTTATAGGCTTCAAGGATGGAGCTGTGGAAGTGAGATGGGCTACTGGTATTACAAGCAAGGTGCCCCCTTGTGAAATTTTCCGGTTTGACAAACATGAAGTTTCAGCTGCACTCCCTGCAGAAGATGACATCGAGGACTTGAACCGAGAGATGATTCAAAATGAGAAGCAACCTTCTAACCAGAAGGGAAAGGGTTTATTGAATTTGGATGATGTCAGTAAAGTTGGAAAAGATTACACAAGGGAGTCTAGTTCCTCTTTCCTTTCTCAAGCTGCCATTGGATTTTTCACTAGCATAGCTGCAAGTCTCTTTGGATCCCAGGAGTCTGTGCCGCTTTCAGCTCCTTCTCCGTCAGTCTGTGTTTCTGAAGTTGGAAATGAATCTGAGATTCCCCATGAGAAAGGAATAGCAGAAACTTGTGAACTCTTTACTGAGCAACAGTCAACGACTGAGCTGGAGAGGTTTGAGGGGAATAGCATTCCACATTCAAAAGCTAATGACAGTGTAGATCAGTTTAGACAGTTTGACATAATTGCTGATTGCACCGACCACCATTTTCATGGTGCTAACAAGGAGTTGGCATTGTCTCAGGTGAAAAGAGGCTGGATGAAAAGGGTTCAACAAGAGTGGAGCATCTTCGAGAAAGATCTGCCTGAACAAATCTATGTCCGCGCCTTCGAGGAAAGGATGGATCTACTGCAAGCAGCCATTGTTGGTGCACCTGGAACTCCATATCATGACGGGCTTTTCTTCTTTGATATTTATCTTCCACCAGAATATCCTCATGAACCGCCTATGGTACACTATACTTCTGGTGGGTTACGTGTGAACCCTAACTTGTACGAGTCCGGAAAGGTCTGTCTCAGTCTTCTTAATACATGGACGGGCACAGGCACTGAAGTTTGGAATCCAGGGGGCTCCACCATTCTTCAAGTTCTTCTCTCCCTTCAAGCCCTCGTGCTTAACGACAAGCCATATTTCAATGAAGCTGGGTATGATCAACAGATAGGAAGAACCGAGGGAGAGAAAAACTCTGTAAGCTACAACGAAAATGCATTCCTCATGACTTGCAAGTCCATGCTCTACACACTCCACAAGCCACCTAAGCATTTCGTGGAGCTTGTGATCGAACACTTCACTCGGCACTCCCAAAATATTCTAATGGCGTGTAAGGCGTATATGGACGGGGCTCCAGTAGGGTGTGCTGTTGGTTTCCAGAAAACTGAAGATAAAGACGGTAAGGGAAGCTCTACCGGGTTCAAAATCATGCTCTCTAAGCTCTTTCCAAAATTGGTTGAGGCATTTTCCGCCAAGGGAATTGACTGCAACCAGTTCATCGGGCCGGAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006464 GO:0006807 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0010966 GO:0012505 GO:0015698 GO:0016036 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0030163 GO:0031625 GO:0031667 GO:0031668 GO:0031669 GO:0032446 GO:0032879 GO:0033554 GO:0034762 GO:0034765 GO:0036211 GO:0042592 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050789 GO:0050801 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0055062 GO:0055081 GO:0055083 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070647 GO:0071496 GO:0071704 GO:0072505 GO:0072506 GO:0098771 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903795 GO:1903959 GO:2000185

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

897

Amino Acids

100.55

Weight (kDa)

5.1

Isoelectric Point (pI)

37.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tSH3-B_UBE2O	PF23046	90 - 222	2.7e-41	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	312 - 385	2.1e-16	UBE2O, SH3-B domain
UQ_con	PF00179	651 - 797	1.6e-23	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33770
fragaria_vesca	FvH4_3g09200 FvH4_3g29950
malus_domestica	MD05G1312700.v1.1 MD10G1294500.v1.1
prunus_persica	Prupe.4G047400_v2.0.a1 Prupe.4G047400_v2.0.a1
pyrus_communis	pycom05g29070 pycom05g29080 pycom10g24620 pycom10g24630 pycom10g24640
rosa_chinensis	RchiOBHm_Chr5g0008401
rosa_laevigata	RLG00000031568
rosa_multiflora	Rmu_co8007308.1_g000001 Rmu_co8292313.1_g000001 Rmu_sc0004326.1_g000003 Rmu_sc0028200.1_g000001 Rmu_ssc0000388.1_g000061
rosa_roxburghii	Rroxscaffold_1G00068090
rosa_rugosa	Rorug04G0435600
rosa_samantha	Rh5AG064200 Rh5BG061400 Rh5CG071300 Rh5DG060200 Rh5DG060300
rosa_wichuraiana	Rw5G005660

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2410
Acc36I	ACCTGC	1 cut(s)	445
AccB1I	GGYRCC	1 cut(s)	1399
AccB7I	CCANNNNNTGG	2 cut(s)	299, 889
AccII	CGCG	1 cut(s)	2010
AccIII	TCCGGA	1 cut(s)	2186
AciI	CCGC	6 cut(s)	498, 1217, 1670, 2008, 2129, 2646
AclWI	GGATC	8 cut(s)	12, 140, 247, 384, 413, 1647, 1660, 2037
AcsI	RAATTY	5 cut(s)	643, 811, 941, 1413, 1540
AcuI	CTGAAG	4 cut(s)	1104, 1725, 2256, 2583
AcvI	CACGTG	1 cut(s)	620
AcyI	GRCGYC	1 cut(s)	68
AfaI	GTAC	5 cut(s)	428, 694, 1094, 2139, 2180
AfeI	AGCGCT	1 cut(s)	1129
AfiI	CCNNNNNNNGG	3 cut(s)	299, 889, 1012
AflIII	ACRYGT	4 cut(s)	183, 619, 993, 2161
AhlI	ACTAGT	1 cut(s)	910
AjnI	CCWGG	5 cut(s)	439, 963, 1656, 2059, 2248
AleI	CACNNNNGTG	1 cut(s)	371
AloI	GAACNNNNNNTCC	2 cut(s)	945, 977
Alw21I	GWGCWC	1 cut(s)	2059
Alw26I	GTCTC	3 cut(s)	1649, 1929, 2204
Alw44I	GTGCAC	1 cut(s)	2055
AlwI	GGATC	8 cut(s)	12, 140, 247, 384, 413, 1647, 1660, 2037
AlwNI	CAGNNNCTG	1 cut(s)	2234
Aor13HI	TCCGGA	1 cut(s)	2186
Aor51HI	AGCGCT	1 cut(s)	1129
AoxI	GGCC	4 cut(s)	289, 442, 947, 2678
ApaLI	GTGCAC	1 cut(s)	2055
ApeKI	GCWGC	6 cut(s)	676, 1262, 1445, 1610, 1637, 2042
ApoI	RAATTY	5 cut(s)	643, 811, 941, 1413, 1540
AspLEI	GCGC	2 cut(s)	1130, 2012
AspS9I	GGNCC	4 cut(s)	290, 849, 1192, 2678
AsuC2I	CCSGG	1 cut(s)	2593
AsuHPI	GGTGA	2 cut(s)	695, 1942
AsuII	TTCGAA	1 cut(s)	632
AvaII	GGWCC	2 cut(s)	849, 1192
BaeGI	GKGCMC	3 cut(s)	1404, 2059, 2228
BamHI	GGATCC	1 cut(s)	1652
BanI	GGYRCC	1 cut(s)	1399
BanII	GRGCYC	2 cut(s)	2258, 2530
BauI	CACGAG	1 cut(s)	2294
BbrPI	CACGTG	1 cut(s)	620
BbsI	GAAGAC	1 cut(s)	2198
Bbv12I	GWGCWC	1 cut(s)	2059
BbvI	GCAGC	6 cut(s)	688, 1249, 1432, 1597, 1624, 2054
BciT130I	CCWGG	5 cut(s)	441, 965, 1658, 2061, 2250
BciVI	GTATCC	1 cut(s)	142
BclI	TGATCA	3 cut(s)	834, 934, 2335
BcnI	CCSGG	1 cut(s)	2593
BcoDI	GTCTC	3 cut(s)	1649, 1929, 2204
BcuI	ACTAGT	1 cut(s)	910
BfaI	CTAG	5 cut(s)	62, 911, 999, 1587, 1629
BfmI	CTRYAG	1 cut(s)	1455
BfoI	RGCGCY	1 cut(s)	1131
BfuAI	ACCTGC	1 cut(s)	445
BfuI	GTATCC	1 cut(s)	142
BglII	AGATCT	1 cut(s)	1984
BisI	GCNGC	7 cut(s)	677, 1263, 1446, 1611, 1638, 1670, 2043
BlsI	GCNGC	7 cut(s)	678, 1264, 1447, 1612, 1639, 1671, 2044
BmcAI	AGTACT	1 cut(s)	1094
Bme1390I	CCNGG	6 cut(s)	441, 965, 1658, 2061, 2250, 2593
Bme18I	GGWCC	2 cut(s)	849, 1192
BmgT120I	GGNCC	4 cut(s)	290, 849, 1192, 2678
BmiI	GGNNCC	4 cut(s)	1401, 1654, 2257, 2529
BmrFI	CCNGG	6 cut(s)	441, 965, 1658, 2061, 2250, 2593
BmsI	GCATC	2 cut(s)	1024, 1980
BpiI	GAAGAC	1 cut(s)	2198
BplI	GAGNNNNNCTC	2 cut(s)	2354, 2386
BpmI	CTGGAG	3 cut(s)	526, 1814, 2514
Bpu10I	CCTNAGC	1 cut(s)	2442
Bpu14I	TTCGAA	1 cut(s)	632
BpuEI	CTTGAG	1 cut(s)	1590
BpuMI	CCSGG	1 cut(s)	2593
Bsa29I	ATCGAT	1 cut(s)	84
BsaAI	YACGTR	2 cut(s)	620, 2162
BsaBI	GATNNNNATC	1 cut(s)	2100
BsaHI	GRCGYC	1 cut(s)	68
BsaJI	CCNNGG	6 cut(s)	293, 1098, 1656, 2249, 2355, 2649
BsaWI	WCCGGW	2 cut(s)	1419, 2186
BsaXI	ACNNNNNCTCC	2 cut(s)	540, 570
Bsc4I	CCNNNNNNNGG	3 cut(s)	299, 889, 1012
Bse1I	ACTGG	9 cut(s)	42, 509, 572, 692, 917, 1143, 1387, 2531, 2668
Bse3DI	GCAATG	1 cut(s)	1067
Bse8I	GATNNNNATC	1 cut(s)	2100
BseAI	TCCGGA	1 cut(s)	2186
BseBI	CCWGG	5 cut(s)	441, 965, 1658, 2061, 2250
BseCI	ATCGAT	1 cut(s)	84
BseDI	CCNNGG	6 cut(s)	293, 1098, 1656, 2249, 2355, 2649
BseGI	GGATG	6 cut(s)	826, 1039, 1360, 1552, 1950, 2031
BseJI	GATNNNNATC	1 cut(s)	2100
BseLI	CCNNNNNNNGG	3 cut(s)	299, 889, 1012
BseMI	GCAATG	1 cut(s)	1067
BseMII	CTCAG	6 cut(s)	437, 1713, 1761, 1779, 1940, 2215
BseNI	ACTGG	9 cut(s)	42, 509, 572, 692, 917, 1143, 1387, 2531, 2668
BseRI	GAGGAG	1 cut(s)	603
BseSI	GKGCMC	3 cut(s)	1404, 2059, 2228
BseXI	GCAGC	6 cut(s)	688, 1249, 1432, 1597, 1624, 2054
BseYI	CCCAGC	1 cut(s)	2327
BsgI	GTGCAG	4 cut(s)	473, 974, 1062, 1431
Bsh1236I	CGCG	1 cut(s)	2010
BshFI	GGCC	4 cut(s)	291, 444, 949, 2680
BshNI	GGYRCC	1 cut(s)	1399
BshVI	ATCGAT	1 cut(s)	84
BsiHKAI	GWGCWC	1 cut(s)	2059
BsiSI	CCGG	4 cut(s)	1420, 2187, 2592, 2681
BslFI	GGGAC	2 cut(s)	137, 287
BslI	CCNNNNNNNGG	3 cut(s)	299, 889, 1012
BsmAI	GTCTC	3 cut(s)	1649, 1929, 2204
BsmFI	GGGAC	2 cut(s)	137, 287
BsmI	GAATGC	2 cut(s)	1815, 2393
BsnI	GGCC	4 cut(s)	291, 444, 949, 2680
Bsp119I	TTCGAA	1 cut(s)	632
Bsp1286I	GDGCHC	5 cut(s)	1404, 2059, 2228, 2258, 2530
Bsp13I	TCCGGA	1 cut(s)	2186
Bsp19I	CCATGG	1 cut(s)	293
BspACI	CCGC	6 cut(s)	498, 1217, 1670, 2008, 2129, 2646
BspANI	GGCC	4 cut(s)	291, 444, 949, 2680
BspCNI	CTCAG	6 cut(s)	436, 1714, 1762, 1780, 1939, 2214
BspDI	ATCGAT	1 cut(s)	84
BspEI	TCCGGA	1 cut(s)	2186
BspFNI	CGCG	1 cut(s)	2010
BspHI	TCATGA	4 cut(s)	937, 2074, 2122, 2400
BspLI	GGNNCC	4 cut(s)	1401, 1654, 2257, 2529
BspMAI	CTGCAG	1 cut(s)	1459
BspMI	ACCTGC	1 cut(s)	445
BspPI	GGATC	8 cut(s)	12, 140, 247, 384, 413, 1647, 1660, 2037
BspT104I	TTCGAA	1 cut(s)	632
BspT107I	GGYRCC	1 cut(s)	1399
BsrDI	GCAATG	1 cut(s)	1067
BsrI	ACTGG	9 cut(s)	42, 509, 572, 692, 917, 1143, 1387, 2531, 2668
BssECI	CCNNGG	6 cut(s)	293, 1098, 1656, 2249, 2355, 2649
BssNI	GRCGYC	1 cut(s)	68
BssSI	CACGAG	1 cut(s)	2294
BssT1I	CCWWGG	3 cut(s)	293, 1098, 2649
Bst2BI	CACGAG	1 cut(s)	2294
Bst2UI	CCWGG	5 cut(s)	441, 965, 1658, 2061, 2250
BstACI	GRCGYC	1 cut(s)	68
BstAPI	GCANNNNNTGC	1 cut(s)	1454
BstBAI	YACGTR	2 cut(s)	620, 2162
BstBI	TTCGAA	1 cut(s)	632
BstC8I	GCNNGC	5 cut(s)	39, 573, 609, 732, 2040
BstDEI	CTNAG	8 cut(s)	423, 1722, 1770, 1788, 1926, 2201, 2442, 2613
BstDSI	CCRYGG	1 cut(s)	293
BstF5I	GGATG	6 cut(s)	826, 1039, 1360, 1552, 1950, 2031
BstFNI	CGCG	1 cut(s)	2010
BstH2I	RGCGCY	1 cut(s)	1131
BstHHI	GCGC	2 cut(s)	1130, 2012
BstMAI	GTCTC	3 cut(s)	1649, 1929, 2204
BstMWI	GCNNNNNNNGC	6 cut(s)	460, 1454, 2443, 2510, 2614, 2645
BstNI	CCWGG	5 cut(s)	441, 965, 1658, 2061, 2250
BstNSI	RCATGY	3 cut(s)	41, 187, 997
BstSCI	CCNGG	6 cut(s)	439, 963, 1656, 2059, 2248, 2591
BstSFI	CTRYAG	1 cut(s)	1455
BstSLI	GKGCMC	3 cut(s)	1404, 2059, 2228
BstUI	CGCG	1 cut(s)	2010
BstV1I	GCAGC	6 cut(s)	688, 1249, 1432, 1597, 1624, 2054
BstV2I	GAAGAC	1 cut(s)	2198
BstX2I	RGATCY	6 cut(s)	145, 252, 418, 1652, 1984, 2029
BstXI	CCANNNNNNTGG	2 cut(s)	268, 1898
BstYI	RGATCY	6 cut(s)	145, 252, 418, 1652, 1984, 2029
Bsu15I	ATCGAT	1 cut(s)	84
BsuI	GTATCC	1 cut(s)	142
BsuRI	GGCC	4 cut(s)	291, 444, 949, 2680
BsuTUI	ATCGAT	1 cut(s)	84
BtgI	CCRYGG	1 cut(s)	293
BtsCI	GGATG	6 cut(s)	826, 1039, 1360, 1552, 1950, 2031
BtsI	GCAGTG	2 cut(s)	366, 1074
BtsIMutI	CAGTG	5 cut(s)	366, 1074, 1305, 1846, 2232
BveI	ACCTGC	1 cut(s)	445
Cac8I	GCNNGC	5 cut(s)	39, 573, 609, 732, 2040
CaiI	CAGNNNCTG	1 cut(s)	2234
CciI	TCATGA	4 cut(s)	937, 2074, 2122, 2400
CfoI	GCGC	2 cut(s)	1130, 2012
Cfr13I	GGNCC	4 cut(s)	290, 849, 1192, 2678
ClaI	ATCGAT	1 cut(s)	84
CseI	GACGC	1 cut(s)	57
Csp6I	GTAC	5 cut(s)	427, 693, 1093, 2138, 2179
CspCI	CAANNNNNGTGG	4 cut(s)	1264, 1299, 1946, 1981
CviQI	GTAC	5 cut(s)	427, 693, 1093, 2138, 2179
DdeI	CTNAG	8 cut(s)	423, 1722, 1770, 1788, 1926, 2201, 2442, 2613
DrdI	GACNNNNNNGTC	1 cut(s)	2410
DseDI	GACNNNNNNGTC	1 cut(s)	2410
EciI	GGCGGA	1 cut(s)	2635
Eco130I	CCWWGG	3 cut(s)	293, 1098, 2649
Eco24I	GRGCYC	2 cut(s)	2258, 2530
Eco47I	GGWCC	2 cut(s)	849, 1192
Eco47III	AGCGCT	1 cut(s)	1129
Eco57I	CTGAAG	4 cut(s)	1104, 1725, 2256, 2583
Eco72I	CACGTG	1 cut(s)	620
EcoRI	GAATTC	1 cut(s)	811
EcoRII	CCWGG	5 cut(s)	439, 963, 1656, 2059, 2248
EcoT14I	CCWWGG	3 cut(s)	293, 1098, 2649
EcoT22I	ATGCAT	1 cut(s)	2395
EcoT38I	GRGCYC	2 cut(s)	2258, 2530
ErhI	CCWWGG	3 cut(s)	293, 1098, 2649
FaqI	GGGAC	2 cut(s)	137, 287
FbaI	TGATCA	3 cut(s)	834, 934, 2335
Fnu4HI	GCNGC	7 cut(s)	677, 1263, 1446, 1611, 1638, 1670, 2043
FokI	GGATG	6 cut(s)	833, 1046, 1367, 1559, 1957, 2038
FriOI	GRGCYC	2 cut(s)	2258, 2530
Fsp4HI	GCNGC	7 cut(s)	677, 1263, 1446, 1611, 1638, 1670, 2043
FspBI	CTAG	5 cut(s)	62, 911, 999, 1587, 1629
GlaI	GCGC	2 cut(s)	1129, 2011
GluI	GCNGC	7 cut(s)	677, 1263, 1446, 1611, 1638, 1670, 2043
GsaI	CCCAGC	1 cut(s)	2331
GsuI	CTGGAG	3 cut(s)	526, 1814, 2514
HaeII	RGCGCY	1 cut(s)	1131
HaeIII	GGCC	4 cut(s)	291, 444, 949, 2680
HapII	CCGG	4 cut(s)	1420, 2187, 2592, 2681
HgaI	GACGC	1 cut(s)	57
HhaI	GCGC	2 cut(s)	1130, 2012
Hin1I	GRCGYC	1 cut(s)	68
Hin6I	GCGC	2 cut(s)	1128, 2010
HinP1I	GCGC	2 cut(s)	1128, 2010
HincII	GTYRAC	3 cut(s)	322, 565, 1782
HindII	GTYRAC	3 cut(s)	322, 565, 1782
HpaII	CCGG	4 cut(s)	1420, 2187, 2592, 2681
HphI	GGTGA	2 cut(s)	695, 1942
Hpy188I	TCNGA	6 cut(s)	600, 784, 798, 1270, 1705, 1723
HpyAV	CCTTC	7 cut(s)	297, 539, 1516, 1521, 1692, 2023, 2294
HpyCH4IV	ACGT	3 cut(s)	98, 619, 2161
HpyF10VI	GCNNNNNNNGC	6 cut(s)	460, 1454, 2443, 2510, 2614, 2645
HpyF3I	CTNAG	8 cut(s)	423, 1722, 1770, 1788, 1926, 2201, 2442, 2613
HpySE526I	ACGT	3 cut(s)	98, 619, 2161
Hsp92I	GRCGYC	1 cut(s)	68
HspAI	GCGC	2 cut(s)	1128, 2010
Kpn2I	TCCGGA	1 cut(s)	2186
Ksp22I	TGATCA	3 cut(s)	834, 934, 2335
LmnI	GCTCC	9 cut(s)	355, 616, 727, 1358, 1684, 1968, 2261, 2455, 2533
Lsp1109I	GCAGC	6 cut(s)	688, 1249, 1432, 1597, 1624, 2054
LweI	GCATC	2 cut(s)	1024, 1980
MaeI	CTAG	5 cut(s)	62, 911, 999, 1587, 1629
MaeII	ACGT	3 cut(s)	98, 619, 2161
MaeIII	GTNAC	7 cut(s)	44, 328, 364, 500, 683, 803, 2157
MflI	RGATCY	6 cut(s)	145, 252, 418, 1652, 1984, 2029
MhlI	GDGCHC	5 cut(s)	1404, 2059, 2228, 2258, 2530
MlyI	GAGTC	3 cut(s)	1592, 1670, 2192
MmeI	TCCRAC	3 cut(s)	1092, 1543, 1690
Mph1103I	ATGCAT	1 cut(s)	2395
MroI	TCCGGA	1 cut(s)	2186
MseI	TTAA	5 cut(s)	180, 765, 1143, 2211, 2301
MslI	CAYNNNNRTG	3 cut(s)	266, 371, 1896
MspA1I	CMGCKG	1 cut(s)	1445
MspI	CCGG	4 cut(s)	1420, 2187, 2592, 2681
MspR9I	CCNGG	6 cut(s)	441, 965, 1658, 2061, 2250, 2593
Mva1269I	GAATGC	2 cut(s)	1815, 2393
MvaI	CCWGG	5 cut(s)	441, 965, 1658, 2061, 2250
MvnI	CGCG	1 cut(s)	2010
MwoI	GCNNNNNNNGC	6 cut(s)	460, 1454, 2443, 2510, 2614, 2645
NciI	CCSGG	1 cut(s)	2593
NcoI	CCATGG	1 cut(s)	293
NlaIV	GGNNCC	4 cut(s)	1401, 1654, 2257, 2529
NmeAIII	GCCGAG	1 cut(s)	2458
NmuCI	GTSAC	2 cut(s)	364, 683
NsiI	ATGCAT	1 cut(s)	2395
NspI	RCATGY	3 cut(s)	41, 187, 997
NspV	TTCGAA	1 cut(s)	632
OliI	CACNNNNGTG	1 cut(s)	371
PaeI	GCATGC	1 cut(s)	41
PagI	TCATGA	4 cut(s)	937, 2074, 2122, 2400
PciI	ACATGT	2 cut(s)	183, 993
PcsI	WCGNNNNNNNCGW	1 cut(s)	2301
PctI	GAATGC	2 cut(s)	1815, 2393
PfeI	GAWTC	7 cut(s)	629, 899, 1198, 1492, 1718, 1726, 2245
PflMI	CCANNNNNTGG	2 cut(s)	299, 889
PfoI	TCCNGGA	1 cut(s)	963
PkrI	GCNGC	7 cut(s)	678, 1264, 1447, 1612, 1639, 1671, 2044
PleI	GAGTC	3 cut(s)	1591, 1669, 2191
PmaCI	CACGTG	1 cut(s)	620
PmlI	CACGTG	1 cut(s)	620
PpsI	GAGTC	3 cut(s)	1591, 1669, 2191
Ppu21I	YACGTR	2 cut(s)	620, 2162
PscI	ACATGT	2 cut(s)	183, 993
Psp6I	CCWGG	5 cut(s)	439, 963, 1656, 2059, 2248
PspCI	CACGTG	1 cut(s)	620
PspFI	CCCAGC	1 cut(s)	2327
PspGI	CCWGG	5 cut(s)	439, 963, 1656, 2059, 2248
PspN4I	GGNNCC	4 cut(s)	1401, 1654, 2257, 2529
PspPI	GGNCC	4 cut(s)	290, 849, 1192, 2678
PstI	CTGCAG	1 cut(s)	1459
PstNI	CAGNNNCTG	1 cut(s)	2234
PsuI	RGATCY	6 cut(s)	145, 252, 418, 1652, 1984, 2029
PvuII	CAGCTG	1 cut(s)	1445
RsaI	GTAC	5 cut(s)	428, 694, 1094, 2139, 2180
RsaNI	GTAC	5 cut(s)	427, 693, 1093, 2138, 2179
RseI	CAYNNNNRTG	3 cut(s)	266, 371, 1896
SaqAI	TTAA	5 cut(s)	180, 765, 1143, 2211, 2301
SatI	GCNGC	7 cut(s)	677, 1263, 1446, 1611, 1638, 1670, 2043
Sau96I	GGNCC	4 cut(s)	290, 849, 1192, 2678
ScaI	AGTACT	1 cut(s)	1094
SchI	GAGTC	3 cut(s)	1592, 1670, 2192
ScrFI	CCNGG	6 cut(s)	441, 965, 1658, 2061, 2250, 2593
SduI	GDGCHC	5 cut(s)	1404, 2059, 2228, 2258, 2530
SfaNI	GCATC	2 cut(s)	1024, 1980
SfcI	CTRYAG	1 cut(s)	1455
SfuI	TTCGAA	1 cut(s)	632
SinI	GGWCC	2 cut(s)	849, 1192
SmiMI	CAYNNNNRTG	3 cut(s)	266, 371, 1896
SmlI	CTYRAG	1 cut(s)	1605
SmoI	CTYRAG	1 cut(s)	1605
SpeI	ACTAGT	1 cut(s)	910
SphI	GCATGC	1 cut(s)	41
SsiI	CCGC	6 cut(s)	498, 1217, 1670, 2008, 2129, 2646
SspI	AATATT	2 cut(s)	23, 2494
SspMI	CTAG	5 cut(s)	62, 911, 999, 1587, 1629
StyD4I	CCNGG	6 cut(s)	439, 963, 1656, 2059, 2248, 2591
StyI	CCWWGG	3 cut(s)	293, 1098, 2649
TaiI	ACGT	3 cut(s)	101, 622, 2164
TaqI	TCGA	7 cut(s)	84, 192, 632, 1470, 1977, 2016, 2466
TatI	WGTACW	1 cut(s)	1092
TauI	GCSGC	1 cut(s)	1672
TfiI	GAWTC	7 cut(s)	629, 899, 1198, 1492, 1718, 1726, 2245
Tru1I	TTAA	5 cut(s)	180, 765, 1143, 2211, 2301
Tru9I	TTAA	5 cut(s)	180, 765, 1143, 2211, 2301
TscAI	CASTG	5 cut(s)	373, 1081, 1312, 1846, 2239
TseFI	GTSAC	2 cut(s)	364, 683
TseI	GCWGC	6 cut(s)	676, 1262, 1445, 1610, 1637, 2042
Tsp45I	GTSAC	2 cut(s)	364, 683
TspGWI	ACGGA	3 cut(s)	245, 368, 1677
TspRI	CASTG	5 cut(s)	373, 1081, 1312, 1846, 2239
Van91I	CCANNNNNTGG	2 cut(s)	299, 889
VneI	GTGCAC	1 cut(s)	2055
VpaK11BI	GGWCC	2 cut(s)	849, 1192
XapI	RAATTY	5 cut(s)	643, 811, 941, 1413, 1540
XceI	RCATGY	3 cut(s)	41, 187, 997
XcmI	CCANNNNNNNNNTGG	2 cut(s)	859, 1895
XspI	CTAG	5 cut(s)	62, 911, 999, 1587, 1629
ZrmI	AGTACT	1 cut(s)	1094
Zsp2I	ATGCAT	1 cut(s)	2395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.