Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006171

Physical Location & Seq

Genomic Coordinates Forward (+)

38245013 .. 38251857

Gene Structure 6845 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1294500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2709 bp

ATGGATCAATATGAAAATGAATCTTTCTATGGTGGGCATGCGGGCAGCATCTTGTCCAGCCTGGAGGCTTCCATTGGGAAAATTGACGATTTTCTCTCGTTTGAGAGGGGATTCATATATGGAGACATGGTATGCTCCGAAAGAGATCCATCTGGGCAGATGGGCAGAGTAGTTGGGATTAATATGTTCGTTGATTTGGAGAGTGTTAAGGGACACATAATAAAAGATGTTAACTCCAATAACCTATCAAAGATACGCTCCATTTCGGTGGGGGATTATGTTGTTTGTGGTCCATGGCTTGGAAGGGTGCATAGAGTGGTTGACCATGTTATGGTTGTATTTGATGATGGAACGGAGTATGAGGTCACTGCTGTTGATCAAGAGAAGCTCTTGCCCATTTCTCCAAACCTACTTGAAGATCCACAGTACCCATATTATCCAGGACAGAAAGTGCAGGTCAGACTCTCAACCGCTTCCAAATCAACTAGATGGTTATGCGGTAACTGGAGGGAAAATCAAGTCGAGGGAACTGTTTGTTCTGTGGAAGGAGGTTTGGTGTATGTTGATTGGCTTGCCTCTGTTCTCATGGGTTGTGATTCGAAATTGCCTGCTCCTCCACGTGTGCTGGATCCGAAAAAGTTGAATGTGTTGTCATGTTTCTCTCATGTAAACTGGCAGCTTGGTGACTGGTGTATGCTTCAAGTTGCTGACGATAAAGCTGTCATGGAGCAGGATTTTCATAGTGCATCTACTTGTGAGATGAATAACAAACCCAAGATATCAGGAAGAGGATTTAGGAGAAGAAACGCAGATTCAAAATTCGATGAAATCTTTGTTATCATAAAGACAAGGACCAAAGTTGATGTGGCGTGGCAAGATGGTAGTCACTCTTTGGGATTAGATTCACAGACACTAGTTCCAGTCAGCGTTGTAAATGATCATGAATTTTGGCCTGAACAGTTTGTCCTGGAAAAGGGCACTTGCGATGATCCACACATGTCTAGCAGCCAAAAATGGGGTGTCGTGAAGGGTGTGGATGCAAACGAGCATACAGTAAAGGTGCAATGGAACACTATCCCTGCACCTAAACAAAATTACTCCGAGGGAAAACAGATGGAGGAAATTGTGAGCGCTTATGAACTAGCTGAGCACCCAGATTACTCCTACTGTTTTGGAGATTATGTGTTCAGGTTGGTCCAGAATCAGTTTGATGAACAAGCGGATAAAAACTATCCGCTCACAAAGATTGACATGAGCGAGGAGGCTGCTTCTGAGGACAAGGACTGTGGTGGGGATCAAGAGGATTACACTGATAAATGTTACCTATCTCACATTGGAAATGTTATGGGCTTCAAGGATGGAGCTGTGGAAGTGAGATGGGCTACTGGTATTACAGGCAAGGTGGCCCCTAATGAAATTTTCCGGTTTGACAAACATGAGGGTTCAGCTGCTATTCCTGCAGAAGATGACATCGAAGACTTGAACCGAGAGATGATTCAAAATGCAAAGCAACCTTCTAACCAGAAGGGAAAGGATTCATTGAATTTGGATGATGGTGGCAAAGATTACACAAGGGAGTCTAGTTCCTCTTTCCTTCCTCAAGCTGCCATTGGATTTTTCACAAGCATAGCTGCGAGTCTTTTGGGATCCCATGAGTCTGCACCACTTTCAGGTCCTTCGTCATCAGCCTGTATTTCTGAAGTTGGAAATGAATCTGAGATTTCCCATGAGAAAGGAATAGCGGAAACTTGTGACCTCTTTACTGAGCAACAGTCAACGACTGAGCTGGAGAGGTTTGAGGAGAAGAGCATTCCACATTCAAAAGCTAATGACAGTGCAGATCAGTTTAGGCAGTTTGACATAGTTGCTGATTGCACCGACCACCATTTTCATGGTGCTAACAAGGAGTTGGCATTATCTCAGGTGAAAAGAGGCTGGATGAAAAAGGTCCAACAAGAGTGGAGCATCTTCAAGAAAGATCTGCCTGAACAAATCTATGTCCGCGCCTTCGAGGAAAGGATGGATCTACTGCGAGCAGCCATTGTTGGTGCACCTGGAACTCCATATCATGATGGGCTTTTCTTCTTTGATATTTATCTTCCACCGGAGTATCCTCATGAACCGCCTATGGTACACTATAGTTCTGGTGGGCTACGTGTCAACCCTAACTTGTACGAGTCCGGAAAGGTCTGTCTCAGTCTCCTTAATACATGGACGGGCACAGGCACTGAAGTTTGGAATCCAGGGAGCTCCACCATTCTTCAAGTTCTTCTCTCCCTTCAAGCCCTCGTGCTTAACGACAAGCCATATTTCAATGAAGCTGGGTATGATCAACAGATAGGAAGAACCCAGGGAGAGAAAAACTCCGTAAGCTACAACGAAAATGCATTCCTGATGACATGCAAGTCCATGCTGTATACACTCCAAAAGCCACCTAAGCATTTTGAGGAGCTTGTTATCGAACACTTCACTCGGCGCTCCCAAAATATTCTAATGGCTTGTAAGGAGTATATGGACGGGGCTCCAGTAGGGTGTGCTATCGATTTCCTGAAAACTGAAGATAAACACAGTAAGGGAAGCTCTACCGGATTCAAAATCATGCTCTCTAAGCTCCTTCCAAAATTGGTTGAGGCATTTTCCAGCAAGGGAATTGACTGCAACCGGTTCATGGGGCCGGAGAAATGTACATGGTGCAAAATTGTAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0006464 GO:0006807 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0010966 GO:0012505 GO:0015698 GO:0016036 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0030163 GO:0031625 GO:0031667 GO:0031668 GO:0031669 GO:0032446 GO:0032879 GO:0033554 GO:0034762 GO:0034765 GO:0036211 GO:0042592 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044444 GO:0044464 GO:0048878 GO:0050789 GO:0050801 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0051716 GO:0055062 GO:0055081 GO:0055083 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0070647 GO:0071496 GO:0071704 GO:0072505 GO:0072506 GO:0098771 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1903795 GO:1903959 GO:2000185

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

903

Amino Acids

101.04

Weight (kDa)

5.04

Isoelectric Point (pI)

38.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tSH3-B_UBE2O	PF23046	90 - 222	6.7e-41	UBE2O, tandem tSH3-B domain
SH3-B_UBE2O	PF23043	313 - 386	9e-17	UBE2O, SH3-B domain
UQ_con	PF00179	650 - 796	2.5e-23	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006171)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G33770
fragaria_vesca	FvH4_3g09200 FvH4_3g29950
malus_domestica	MD05G1312700.v1.1 MD10G1294500.v1.1
prunus_persica	Prupe.4G047400_v2.0.a1 Prupe.4G047400_v2.0.a1
pyrus_communis	pycom05g29070 pycom05g29080 pycom10g24620 pycom10g24630 pycom10g24640
rosa_chinensis	RchiOBHm_Chr5g0008401
rosa_laevigata	RLG00000031568
rosa_multiflora	Rmu_co8007308.1_g000001 Rmu_co8292313.1_g000001 Rmu_sc0004326.1_g000003 Rmu_sc0028200.1_g000001 Rmu_ssc0000388.1_g000061
rosa_roxburghii	Rroxscaffold_1G00068090
rosa_rugosa	Rorug04G0435600
rosa_samantha	Rh5AG064200 Rh5BG061400 Rh5CG071300 Rh5DG060200 Rh5DG060300
rosa_wichuraiana	Rw5G005660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2404
Acc36I	ACCTGC	1 cut(s)	445
AccB7I	CCANNNNNTGG	2 cut(s)	299, 331
AccBSI	CCGCTC	1 cut(s)	1237
AccI	GTMKAC	1 cut(s)	2417
AccII	CGCG	1 cut(s)	2004
AccIII	TCCGGA	1 cut(s)	2180
AciI	CCGC	8 cut(s)	41, 471, 498, 1220, 1235, 1742, 2002, 2123
AcsI	RAATTY	4 cut(s)	818, 944, 1416, 1543
AcuI	CTGAAG	3 cut(s)	1719, 2250, 2577
AcvI	CACGTG	1 cut(s)	620
AfaI	GTAC	4 cut(s)	428, 2133, 2174, 2686
AfeI	AGCGCT	1 cut(s)	1132
AfiI	CCNNNNNNNGG	6 cut(s)	299, 331, 973, 1015, 1670, 2668
AflIII	ACRYGT	3 cut(s)	619, 996, 2155
AgeI	ACCGGT	1 cut(s)	2661
AhlI	ACTAGT	1 cut(s)	913
AjnI	CCWGG	6 cut(s)	60, 439, 966, 2053, 2242, 2349
AloI	GAACNNNNNNTCC	2 cut(s)	948, 980
Alw21I	GWGCWC	3 cut(s)	1152, 2053, 2252
Alw26I	GTCTC	3 cut(s)	117, 2198, 2204
Alw44I	GTGCAC	1 cut(s)	2049
AlwNI	CAGNNNCTG	1 cut(s)	2228
Aor13HI	TCCGGA	1 cut(s)	2180
Aor51HI	AGCGCT	1 cut(s)	1132
AoxI	GGCC	3 cut(s)	950, 1404, 2672
ApaLI	GTGCAC	1 cut(s)	2049
ApeKI	GCWGC	8 cut(s)	45, 676, 1005, 1265, 1448, 1604, 1631, 2036
ApoI	RAATTY	4 cut(s)	818, 944, 1416, 1543
AseI	ATTAAT	1 cut(s)	180
AsiGI	ACCGGT	1 cut(s)	2661
Asp700I	GAANNNNTTC	1 cut(s)	1534
AspLEI	GCGC	3 cut(s)	1133, 2006, 2478
AspS9I	GGNCC	7 cut(s)	290, 852, 1195, 1405, 1673, 1948, 2672
AsuHPI	GGTGA	2 cut(s)	695, 1936
AsuII	TTCGAA	1 cut(s)	599
AvaII	GGWCC	5 cut(s)	290, 852, 1195, 1673, 1948
BaeGI	GKGCMC	3 cut(s)	980, 2053, 2222
BaeI	ACNNNNGTAYC	2 cut(s)	2093, 2126
BamHI	GGATCC	2 cut(s)	628, 1646
BanII	GRGCYC	2 cut(s)	2252, 2524
BauI	CACGAG	1 cut(s)	2288
BbrPI	CACGTG	1 cut(s)	620
BbsI	GAAGAC	1 cut(s)	1482
Bbv12I	GWGCWC	3 cut(s)	1152, 2053, 2252
BbvI	GCAGC	8 cut(s)	57, 688, 1017, 1252, 1435, 1591, 1618, 2048
BcgI	CGANNNNNNTGC	2 cut(s)	1247, 1281
BciT130I	CCWGG	6 cut(s)	62, 441, 968, 2055, 2244, 2351
BciVI	GTATCC	1 cut(s)	2121
BclI	TGATCA	3 cut(s)	376, 937, 2329
BcoDI	GTCTC	3 cut(s)	117, 2198, 2204
BcuI	ACTAGT	1 cut(s)	913
BfaI	CTAG	5 cut(s)	486, 914, 1002, 1142, 1581
BfmI	CTRYAG	2 cut(s)	1458, 2137
BfoI	RGCGCY	2 cut(s)	1134, 2479
BfuAI	ACCTGC	1 cut(s)	445
BfuI	GTATCC	1 cut(s)	2121
BglII	AGATCT	1 cut(s)	1978
BisI	GCNGC	8 cut(s)	46, 677, 1006, 1266, 1449, 1605, 1632, 2037
BlpI	GCTNAGC	1 cut(s)	1146
BlsI	GCNGC	8 cut(s)	47, 678, 1007, 1267, 1450, 1606, 1633, 2038
Bme1390I	CCNGG	6 cut(s)	62, 441, 968, 2055, 2244, 2351
Bme18I	GGWCC	5 cut(s)	290, 852, 1195, 1673, 1948
BmgT120I	GGNCC	7 cut(s)	290, 852, 1195, 1405, 1673, 1948, 2672
BmiI	GGNNCC	5 cut(s)	630, 1407, 1648, 2523, 2673
BmrFI	CCNGG	6 cut(s)	62, 441, 968, 2055, 2244, 2351
BmsI	GCATC	4 cut(s)	57, 755, 1027, 1974
BpiI	GAAGAC	1 cut(s)	1482
BplI	GAGNNNNNCTC	2 cut(s)	2348, 2380
BpmI	CTGGAG	4 cut(s)	83, 526, 1808, 2508
Bpu10I	CCTNAGC	1 cut(s)	2436
Bpu1102I	GCTNAGC	1 cut(s)	1146
Bpu14I	TTCGAA	1 cut(s)	599
BpuEI	CTTGAG	1 cut(s)	1584
Bsa29I	ATCGAT	1 cut(s)	2541
BsaAI	YACGTR	2 cut(s)	620, 2156
BsaBI	GATNNNNATC	1 cut(s)	2094
BsaJI	CCNNGG	5 cut(s)	293, 1101, 2243, 2349, 2350
BsaWI	WCCGGW	5 cut(s)	1422, 2104, 2180, 2585, 2661
BsaXI	ACNNNNNCTCC	6 cut(s)	114, 144, 540, 570, 1167, 1197
Bsc4I	CCNNNNNNNGG	6 cut(s)	299, 331, 973, 1015, 1670, 2668
Bse118I	RCCGGY	1 cut(s)	2661
Bse1I	ACTGG	6 cut(s)	509, 677, 692, 920, 1390, 2525
Bse3DI	GCAATG	1 cut(s)	1070
Bse8I	GATNNNNATC	1 cut(s)	2094
BseAI	TCCGGA	1 cut(s)	2180
BseBI	CCWGG	6 cut(s)	62, 441, 968, 2055, 2244, 2351
BseCI	ATCGAT	1 cut(s)	2541
BseDI	CCNNGG	5 cut(s)	293, 1101, 2243, 2349, 2350
BseGI	GGATG	5 cut(s)	1042, 1363, 1555, 1944, 2025
BseJI	GATNNNNATC	1 cut(s)	2094
BseLI	CCNNNNNNNGG	6 cut(s)	299, 331, 973, 1015, 1670, 2668
BseMI	GCAATG	1 cut(s)	1070
BseMII	CTCAG	7 cut(s)	1137, 1263, 1707, 1755, 1773, 1934, 2209
BseNI	ACTGG	6 cut(s)	509, 677, 692, 920, 1390, 2525
BseRI	GAGGAG	4 cut(s)	603, 1274, 1814, 2462
BseSI	GKGCMC	3 cut(s)	980, 2053, 2222
BseXI	GCAGC	8 cut(s)	57, 688, 1017, 1252, 1435, 1591, 1618, 2048
BseYI	CCCAGC	1 cut(s)	2321
BsgI	GTGCAG	4 cut(s)	473, 1065, 1644, 1857
Bsh1236I	CGCG	1 cut(s)	2004
BshFI	GGCC	3 cut(s)	952, 1406, 2674
BshTI	ACCGGT	1 cut(s)	2661
BshVI	ATCGAT	1 cut(s)	2541
BsiHKAI	GWGCWC	3 cut(s)	1152, 2053, 2252
BsiSI	CCGG	6 cut(s)	1423, 2105, 2181, 2586, 2662, 2675
BslFI	GGGAC	1 cut(s)	225
BslI	CCNNNNNNNGG	6 cut(s)	299, 331, 973, 1015, 1670, 2668
BsmAI	GTCTC	3 cut(s)	117, 2198, 2204
BsmFI	GGGAC	1 cut(s)	225
BsmI	GAATGC	2 cut(s)	1809, 2387
BsnI	GGCC	3 cut(s)	952, 1406, 2674
Bsp119I	TTCGAA	1 cut(s)	599
Bsp1286I	GDGCHC	6 cut(s)	980, 1152, 2053, 2222, 2252, 2524
Bsp13I	TCCGGA	1 cut(s)	2180
Bsp1407I	TGTACA	1 cut(s)	2684
Bsp1720I	GCTNAGC	1 cut(s)	1146
Bsp19I	CCATGG	1 cut(s)	293
BspACI	CCGC	8 cut(s)	41, 471, 498, 1220, 1235, 1742, 2002, 2123
BspANI	GGCC	3 cut(s)	952, 1406, 2674
BspCNI	CTCAG	7 cut(s)	1138, 1264, 1708, 1756, 1774, 1933, 2208
BspDI	ATCGAT	1 cut(s)	2541
BspEI	TCCGGA	1 cut(s)	2180
BspFNI	CGCG	1 cut(s)	2004
BspHI	TCATGA	3 cut(s)	940, 2068, 2116
BspLI	GGNNCC	5 cut(s)	630, 1407, 1648, 2523, 2673
BspMAI	CTGCAG	1 cut(s)	1462
BspMI	ACCTGC	1 cut(s)	445
BspQI	GCTCTTC	1 cut(s)	1799
BspT104I	TTCGAA	1 cut(s)	599
BsrBI	CCGCTC	1 cut(s)	1237
BsrDI	GCAATG	1 cut(s)	1070
BsrFI	RCCGGY	1 cut(s)	2661
BsrGI	TGTACA	1 cut(s)	2684
BsrI	ACTGG	6 cut(s)	509, 677, 692, 920, 1390, 2525
BssAI	RCCGGY	1 cut(s)	2661
BssECI	CCNNGG	5 cut(s)	293, 1101, 2243, 2349, 2350
BssNAI	GTATAC	1 cut(s)	2418
BssSI	CACGAG	1 cut(s)	2288
BssT1I	CCWWGG	1 cut(s)	293
Bst1107I	GTATAC	1 cut(s)	2418
Bst2BI	CACGAG	1 cut(s)	2288
Bst2UI	CCWGG	6 cut(s)	62, 441, 968, 2055, 2244, 2351
Bst4CI	ACNGT	9 cut(s)	426, 532, 960, 1054, 1169, 1286, 1773, 1835, 2570
Bst6I	CTCTTC	2 cut(s)	781, 1799
BstAUI	TGTACA	1 cut(s)	2684
BstBAI	YACGTR	2 cut(s)	620, 2156
BstBI	TTCGAA	1 cut(s)	599
BstC8I	GCNNGC	5 cut(s)	39, 43, 573, 609, 2034
BstDEI	CTNAG	9 cut(s)	1146, 1272, 1716, 1764, 1782, 1920, 2195, 2436, 2607
BstDSI	CCRYGG	1 cut(s)	293
BstENI	CCTNNNNNAGG	1 cut(s)	971
BstF5I	GGATG	5 cut(s)	1042, 1363, 1555, 1944, 2025
BstFNI	CGCG	1 cut(s)	2004
BstH2I	RGCGCY	2 cut(s)	1134, 2479
BstHHI	GCGC	3 cut(s)	1133, 2006, 2478
BstMAI	GTCTC	3 cut(s)	117, 2198, 2204
BstMWI	GCNNNNNNNGC	3 cut(s)	1457, 2437, 2608
BstNI	CCWGG	6 cut(s)	62, 441, 968, 2055, 2244, 2351
BstNSI	RCATGY	3 cut(s)	41, 1000, 2403
BstSCI	CCNGG	6 cut(s)	60, 439, 966, 2053, 2242, 2349
BstSFI	CTRYAG	2 cut(s)	1458, 2137
BstSLI	GKGCMC	3 cut(s)	980, 2053, 2222
BstUI	CGCG	1 cut(s)	2004
BstV1I	GCAGC	8 cut(s)	57, 688, 1017, 1252, 1435, 1591, 1618, 2048
BstV2I	GAAGAC	1 cut(s)	1482
BstX2I	RGATCY	6 cut(s)	145, 418, 628, 1646, 1978, 2023
BstXI	CCANNNNNNTGG	2 cut(s)	268, 1892
BstYI	RGATCY	6 cut(s)	145, 418, 628, 1646, 1978, 2023
BstZ17I	GTATAC	1 cut(s)	2418
Bsu15I	ATCGAT	1 cut(s)	2541
BsuI	GTATCC	1 cut(s)	2121
BsuRI	GGCC	3 cut(s)	952, 1406, 2674
BsuTUI	ATCGAT	1 cut(s)	2541
BtgI	CCRYGG	1 cut(s)	293
BtgZI	GCGATG	1 cut(s)	999
BtsCI	GGATG	5 cut(s)	1042, 1363, 1555, 1944, 2025
BtsI	GCAGTG	1 cut(s)	366
BtsIMutI	CAGTG	4 cut(s)	366, 1308, 1840, 2226
BveI	ACCTGC	1 cut(s)	445
Cac8I	GCNNGC	5 cut(s)	39, 43, 573, 609, 2034
CaiI	CAGNNNCTG	1 cut(s)	2228
CciI	TCATGA	3 cut(s)	940, 2068, 2116
CfoI	GCGC	3 cut(s)	1133, 2006, 2478
Cfr10I	RCCGGY	1 cut(s)	2661
Cfr13I	GGNCC	7 cut(s)	290, 852, 1195, 1405, 1673, 1948, 2672
ClaI	ATCGAT	1 cut(s)	2541
Csp6I	GTAC	4 cut(s)	427, 2132, 2173, 2685
CspAI	ACCGGT	1 cut(s)	2661
CspCI	CAANNNNNGTGG	4 cut(s)	1267, 1302, 1940, 1975
CviQI	GTAC	4 cut(s)	427, 2132, 2173, 2685
DdeI	CTNAG	9 cut(s)	1146, 1272, 1716, 1764, 1782, 1920, 2195, 2436, 2607
DrdI	GACNNNNNNGTC	1 cut(s)	2404
DseDI	GACNNNNNNGTC	1 cut(s)	2404
Eam1104I	CTCTTC	2 cut(s)	781, 1799
EarI	CTCTTC	2 cut(s)	781, 1799
Ecl136II	GAGCTC	1 cut(s)	2250
Eco130I	CCWWGG	1 cut(s)	293
Eco24I	GRGCYC	2 cut(s)	2252, 2524
Eco32I	GATATC	1 cut(s)	780
Eco47I	GGWCC	5 cut(s)	290, 852, 1195, 1673, 1948
Eco47III	AGCGCT	1 cut(s)	1132
Eco53kI	GAGCTC	1 cut(s)	2250
Eco57I	CTGAAG	3 cut(s)	1719, 2250, 2577
Eco72I	CACGTG	1 cut(s)	620
EcoICRI	GAGCTC	1 cut(s)	2250
EcoNI	CCTNNNNNAGG	1 cut(s)	971
EcoO109I	RGGNCCY	1 cut(s)	1673
EcoRII	CCWGG	6 cut(s)	60, 439, 966, 2053, 2242, 2349
EcoRV	GATATC	1 cut(s)	780
EcoT14I	CCWWGG	1 cut(s)	293
EcoT22I	ATGCAT	1 cut(s)	2389
EcoT38I	GRGCYC	2 cut(s)	2252, 2524
ErhI	CCWWGG	1 cut(s)	293
FaqI	GGGAC	1 cut(s)	225
FauI	CCCGC	1 cut(s)	34
FbaI	TGATCA	3 cut(s)	376, 937, 2329
FblI	GTMKAC	1 cut(s)	2417
Fnu4HI	GCNGC	8 cut(s)	46, 677, 1006, 1266, 1449, 1605, 1632, 2037
FokI	GGATG	5 cut(s)	1049, 1370, 1562, 1951, 2032
FriOI	GRGCYC	2 cut(s)	2252, 2524
Fsp4HI	GCNGC	8 cut(s)	46, 677, 1006, 1266, 1449, 1605, 1632, 2037
FspBI	CTAG	5 cut(s)	486, 914, 1002, 1142, 1581
GlaI	GCGC	3 cut(s)	1132, 2005, 2477
GluI	GCNGC	8 cut(s)	46, 677, 1006, 1266, 1449, 1605, 1632, 2037
GsaI	CCCAGC	1 cut(s)	2325
GsuI	CTGGAG	4 cut(s)	83, 526, 1808, 2508
HaeII	RGCGCY	2 cut(s)	1134, 2479
HaeIII	GGCC	3 cut(s)	952, 1406, 2674
HapII	CCGG	6 cut(s)	1423, 2105, 2181, 2586, 2662, 2675
HhaI	GCGC	3 cut(s)	1133, 2006, 2478
Hin6I	GCGC	3 cut(s)	1131, 2004, 2476
HinP1I	GCGC	3 cut(s)	1131, 2004, 2476
HincII	GTYRAC	4 cut(s)	232, 322, 1776, 2161
HindII	GTYRAC	4 cut(s)	232, 322, 1776, 2161
HpaI	GTTAAC	1 cut(s)	232
HpaII	CCGG	6 cut(s)	1423, 2105, 2181, 2586, 2662, 2675
HphI	GGTGA	2 cut(s)	695, 1936
Hpy166II	GTNNAC	8 cut(s)	232, 322, 670, 1776, 2051, 2134, 2161, 2418
Hpy188I	TCNGA	7 cut(s)	139, 461, 633, 1102, 1273, 1699, 1717
Hpy8I	GTNNAC	8 cut(s)	232, 322, 670, 1776, 2051, 2134, 2161, 2418
HpyCH4III	ACNGT	9 cut(s)	426, 532, 960, 1054, 1169, 1286, 1773, 1835, 2570
HpyCH4IV	ACGT	2 cut(s)	619, 2155
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1457, 2437, 2608
HpyF3I	CTNAG	9 cut(s)	1146, 1272, 1716, 1764, 1782, 1920, 2195, 2436, 2607
HpySE526I	ACGT	2 cut(s)	619, 2155
HspAI	GCGC	3 cut(s)	1131, 2004, 2476
Kpn2I	TCCGGA	1 cut(s)	2180
Ksp22I	TGATCA	3 cut(s)	376, 937, 2329
KspAI	GTTAAC	1 cut(s)	232
LguI	GCTCTTC	1 cut(s)	1799
Lsp1109I	GCAGC	8 cut(s)	57, 688, 1017, 1252, 1435, 1591, 1618, 2048
LweI	GCATC	4 cut(s)	57, 755, 1027, 1974
MaeI	CTAG	5 cut(s)	486, 914, 1002, 1142, 1581
MaeII	ACGT	2 cut(s)	619, 2155
MaeIII	GTNAC	6 cut(s)	364, 500, 683, 884, 1319, 1751
MbiI	CCGCTC	1 cut(s)	1237
MflI	RGATCY	6 cut(s)	145, 418, 628, 1646, 1978, 2023
MhlI	GDGCHC	6 cut(s)	980, 1152, 2053, 2222, 2252, 2524
MlyI	GAGTC	5 cut(s)	456, 1586, 1645, 1664, 2186
MmeI	TCCRAC	2 cut(s)	1684, 1975
Mph1103I	ATGCAT	1 cut(s)	2389
MroI	TCCGGA	1 cut(s)	2180
MroXI	GAANNNNTTC	1 cut(s)	1534
MseI	TTAA	5 cut(s)	180, 207, 231, 2205, 2295
MslI	CAYNNNNRTG	2 cut(s)	266, 1890
MspA1I	CMGCKG	1 cut(s)	1448
MspI	CCGG	6 cut(s)	1423, 2105, 2181, 2586, 2662, 2675
MspR9I	CCNGG	6 cut(s)	62, 441, 968, 2055, 2244, 2351
Mva1269I	GAATGC	2 cut(s)	1809, 2387
MvaI	CCWGG	6 cut(s)	62, 441, 968, 2055, 2244, 2351
MvnI	CGCG	1 cut(s)	2004
MwoI	GCNNNNNNNGC	3 cut(s)	1457, 2437, 2608
NcoI	CCATGG	1 cut(s)	293
NlaIV	GGNNCC	5 cut(s)	630, 1407, 1648, 2523, 2673
NmeAIII	GCCGAG	1 cut(s)	2452
NmuCI	GTSAC	4 cut(s)	364, 683, 884, 1751
NsiI	ATGCAT	1 cut(s)	2389
NspI	RCATGY	3 cut(s)	41, 1000, 2403
NspV	TTCGAA	1 cut(s)	599
PaeI	GCATGC	1 cut(s)	41
PagI	TCATGA	3 cut(s)	940, 2068, 2116
PasI	CCCWGGG	1 cut(s)	2350
PciI	ACATGT	1 cut(s)	996
PciSI	GCTCTTC	1 cut(s)	1799
PcsI	WCGNNNNNNNCGW	1 cut(s)	2295
PctI	GAATGC	2 cut(s)	1809, 2387
PdmI	GAANNNNTTC	1 cut(s)	1534
PflMI	CCANNNNNTGG	2 cut(s)	299, 331
PfoI	TCCNGGA	2 cut(s)	439, 966
PinAI	ACCGGT	1 cut(s)	2661
PkrI	GCNGC	8 cut(s)	47, 678, 1007, 1267, 1450, 1606, 1633, 2038
PleI	GAGTC	5 cut(s)	456, 1585, 1644, 1663, 2185
PmaCI	CACGTG	1 cut(s)	620
PmlI	CACGTG	1 cut(s)	620
PpsI	GAGTC	5 cut(s)	456, 1585, 1644, 1663, 2185
Ppu21I	YACGTR	2 cut(s)	620, 2156
PpuMI	RGGWCCY	1 cut(s)	1673
PscI	ACATGT	1 cut(s)	996
PshBI	ATTAAT	1 cut(s)	180
Psp124BI	GAGCTC	1 cut(s)	2252
Psp5II	RGGWCCY	1 cut(s)	1673
Psp6I	CCWGG	6 cut(s)	60, 439, 966, 2053, 2242, 2349
PspCI	CACGTG	1 cut(s)	620
PspFI	CCCAGC	1 cut(s)	2321
PspGI	CCWGG	6 cut(s)	60, 439, 966, 2053, 2242, 2349
PspN4I	GGNNCC	5 cut(s)	630, 1407, 1648, 2523, 2673
PspPI	GGNCC	7 cut(s)	290, 852, 1195, 1405, 1673, 1948, 2672
PspPPI	RGGWCCY	1 cut(s)	1673
PstI	CTGCAG	1 cut(s)	1462
PstNI	CAGNNNCTG	1 cut(s)	2228
PsuI	RGATCY	6 cut(s)	145, 418, 628, 1646, 1978, 2023
PvuII	CAGCTG	1 cut(s)	1448
RsaI	GTAC	4 cut(s)	428, 2133, 2174, 2686
RsaNI	GTAC	4 cut(s)	427, 2132, 2173, 2685
RseI	CAYNNNNRTG	2 cut(s)	266, 1890
SacI	GAGCTC	1 cut(s)	2252
SapI	GCTCTTC	1 cut(s)	1799
SaqAI	TTAA	5 cut(s)	180, 207, 231, 2205, 2295
SatI	GCNGC	8 cut(s)	46, 677, 1006, 1266, 1449, 1605, 1632, 2037
Sau96I	GGNCC	7 cut(s)	290, 852, 1195, 1405, 1673, 1948, 2672
SchI	GAGTC	5 cut(s)	456, 1586, 1645, 1664, 2186
ScrFI	CCNGG	6 cut(s)	62, 441, 968, 2055, 2244, 2351
SduI	GDGCHC	6 cut(s)	980, 1152, 2053, 2222, 2252, 2524
SfaNI	GCATC	4 cut(s)	57, 755, 1027, 1974
SfcI	CTRYAG	2 cut(s)	1458, 2137
SfuI	TTCGAA	1 cut(s)	599
SinI	GGWCC	5 cut(s)	290, 852, 1195, 1673, 1948
SmiMI	CAYNNNNRTG	2 cut(s)	266, 1890
SmlI	CTYRAG	1 cut(s)	1599
SmoI	CTYRAG	1 cut(s)	1599
SpeI	ACTAGT	1 cut(s)	913
SphI	GCATGC	1 cut(s)	41
SsiI	CCGC	8 cut(s)	41, 471, 498, 1220, 1235, 1742, 2002, 2123
SspI	AATATT	1 cut(s)	2488
SspMI	CTAG	5 cut(s)	486, 914, 1002, 1142, 1581
SstI	GAGCTC	1 cut(s)	2252
StyD4I	CCNGG	6 cut(s)	60, 439, 966, 2053, 2242, 2349
StyI	CCWWGG	1 cut(s)	293
TaaI	ACNGT	9 cut(s)	426, 532, 960, 1054, 1169, 1286, 1773, 1835, 2570
TaiI	ACGT	2 cut(s)	622, 2158
TaqI	TCGA	7 cut(s)	522, 599, 822, 1473, 2010, 2460, 2541
TatI	WGTACW	1 cut(s)	2684
Tru1I	TTAA	5 cut(s)	180, 207, 231, 2205, 2295
Tru9I	TTAA	5 cut(s)	180, 207, 231, 2205, 2295
TscAI	CASTG	4 cut(s)	373, 1315, 1840, 2233
TseFI	GTSAC	4 cut(s)	364, 683, 884, 1751
TseI	GCWGC	8 cut(s)	45, 676, 1005, 1265, 1448, 1604, 1631, 2036
Tsp45I	GTSAC	4 cut(s)	364, 683, 884, 1751
TspGWI	ACGGA	2 cut(s)	368, 2356
TspRI	CASTG	4 cut(s)	373, 1315, 1840, 2233
Van91I	CCANNNNNTGG	2 cut(s)	299, 331
VneI	GTGCAC	1 cut(s)	2049
VpaK11BI	GGWCC	5 cut(s)	290, 852, 1195, 1673, 1948
VspI	ATTAAT	1 cut(s)	180
XagI	CCTNNNNNAGG	1 cut(s)	971
XapI	RAATTY	4 cut(s)	818, 944, 1416, 1543
XceI	RCATGY	3 cut(s)	41, 1000, 2403
XcmI	CCANNNNNNNNNTGG	2 cut(s)	862, 1889
XmiI	GTMKAC	1 cut(s)	2417
XmnI	GAANNNNTTC	1 cut(s)	1534
XspI	CTAG	5 cut(s)	486, 914, 1002, 1142, 1581
Zsp2I	ATGCAT	1 cut(s)	2389

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.