Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0003023

Physical Location & Seq

Genomic Coordinates Forward (+)

5757936 .. 5767536

Gene Structure 9601 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g09910.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2745 bp

ATGGACAATGCGGACGTCGAGGCCGGCGGAGACGACGAGTTCCAAGGCCGGAGAGGCCGCAAGTACCGCCCCGTGGTCGACGACGACGATCGCGCCGTGCTTGAGATGTCGTCCATGGATCCTTCGTCGTCTTCTTCTTCTTCGTCTCATCCCGCTCAATCGTCGCTCAAGAAAATAAAAGTGAGCTCTGAAGAAAACCTTGCCTCTAACATTAATGAAGGGCCCTCTGCTAGTCATGTGCAATCCAACGGCCCCCAGACAGAATCCAAATTGGAACTCTTTGGCTTTGATTCTCTTGTCAATATTCTCGGTCTTAAGAGTATGACAGATGAGCAAGCGGCAGTACCGTCGAGTCCTAGAGATGGTGAAACTATTGCTATCACTCAGGGGATACCGAAGATAACTGCCATCAAATCGGGTACATTGATGGGTGTATTTGTCCCATGCCTGCAAAACATATTGGGAATTATATACTACATCCGTTTTTCTTGGATTGTTGGTATGGCTGGCATAGCTGATTCACTGTTGTTGGTTACATTATGTGGCACATGTACTTTCCTGACTGGAATATCATTGAGTGCCATTGCAACCAATGGTGCTATGAAGGGAGGTGGACCATACTATCTAATTGGTCGTGCGCTTGGTCCAGAAGTTGGAGTTAGCATTGGGTTATGTTTCTTTCTAGGAAATGCTGTTGCTGGATCTCTTTATGTCTTGGGAGCTGTGGAAACCTTCTTGAAAGCTGTCCCAGGCGCTGGGTTTTTCACTGTAACAACAACGGTTAACGGCATGAAAGTTCAAAGTGCGAGTTTGCATGATTTGCAAATCTATGGGATAATTGTGACCATCCTTTTATGCTTTATTGTGTTTGGGGGAGTGAAAATAATTAATAAGGTTGCACCCGCATTCCTCATACCTGTTTTATTGTCGATGCTCTGCATCTACATTGGGATTGCTCGGGCGAAGAAGAATGACCCAACAGAGGGACTCACGGGATTTAGTTTGGATACATTCAAAGATAATTGGAACTCGCAGTATCAGAAGACAAATGACGCTGGCATTCCTGATCCTGATGGAAAAGTCCCCTGGGACTTCAATGCAATGGTCGGTCTCTTTTTCCCTGCCGTGACGGGAATTATGGCAGGCTCAAATCGATCAGCCTCACTGAAAGACACTCAGCGGTCCATTCCTATTGGAACATTGGCTGCAACTCTTACAACTACTTTTATGTATTTGATCTCCGTGTTATTGTTTGGAGCCATTGCCACCAGACATTTGCTTTTGACCGACAGGCTACTTTCGGCTACAATATCTTGGCCTTACCCAGCATTCATTTACGTAGGAATAATTCTTTCAACCTTAGGTGCTGCTCTTCAAAGCCTGACTGGTGCTCCCCGTCTGCTTGCAGCAATAGCCAATGATGATATCTTACCTGTTCTTAACTACTTCAAGGTTGCAGATGGGAGTGAACCTCACTTCGCTACCTTATTTACCGCATTCCTTTGTATTGCGTGTGTTATAATTGGGAATCTGGATCTTATCACACCAACTATAACTATGTTTTTCCTTCTGTGTTATGCGGGTGTGAACTTATCTTGCTTCCTTCTGGATCTCCTAGATGCTCCCAGCTGGCGTCCTAGGTGGAAATTCCACCATTGGAGCCTCTCTCTTCTTGGAGCTACACTATGTATAGTTATCATGTTCTTGATCTCTTGGGCATTTACCGTAGTGTCTCTAGCCCTTGCAAGCCTTATATATTATTATGTGAGCATCAAAGGAAAGGCTGGGGACTGGGGTGATGGTTTCAAGAGTGCATATTTTCAGCTAGCTCTCCGAAGTCTTCGATCGCTGGGAGCAAACCAAGTGCACCCAAAGAATTGGTATCCCATCCCCCTGATATTCTGCCGGCCTTGGGGGAAGCTGCCAGAGAATGTTCCCTGCCATCCTAAACTTGCCGACTTCGCCAACTGTATGAAGAAGAAGGGCAGGGGAATGTCTATCTTTGTGTCTATCCTAGACGGTGATTACCCTGAATGTGTTGAAGATGCCAAGGCTGCCTGCAAGCAGCTTGATGCTTACATTGAGTACAAGCACTGTGAAGGTGTAGCTGAGATTGTTGTCGCCCCCAGTATGTCTGAAGGTTTTCGCGGCATTGTCCAAACAATGGGTCTTGGCAATCTCAAGCCAAACATTGTGGTAATGCGGTATCCTGAAATATGGCGTCGTGAAAACTTAACAGAAATTCCGAACACCTTTGTTGAAATAATAAATGACTGCATAGTTGCAAACAAGGCAGTAGTTATTGTCAAAGGTCTCGACGAATGGCCCAATGAGTTTCAGAGGCAGTATGGTACTATTGATCTGTATTGGATTGTAAGAGATGGAGGTCTCATGCTTCTTCTTTCTCAACTCCTCCTCACTAAGGAAAGCTTTGAAAGTTGTAAAATTCAAGTTTTCTGCATTGCGGAGGAGGACACTGATGCAGAGGGGCTCAAGGCTGATGTGAAGAAGTTTCTGTATGATCTTCGAATGCATGCAGAAGTCATAGTTGTAACCATGAAATCGTGGGATATGCAAGCAGACAATGGGGCTCCTCAAGATGAATCTGTGGAGGCGTATAATGCTGCTCAAAAGCGGATAGCTGATTACTTGGCTGAGATGAAGTCAACATCTAAGAAACATGGGACTCCGCTGATGGCGGATGGGAAACAGATACTCAAGAATGGCTGCAGTTGTACTCGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006820 GO:0006821 GO:0008150 GO:0008324 GO:0008509 GO:0008511 GO:0009674 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015103 GO:0015108 GO:0015291 GO:0015293 GO:0015294 GO:0015296 GO:0015318 GO:0015370 GO:0015373 GO:0015377 GO:0015378 GO:0015379 GO:0015672 GO:0015698 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0034220 GO:0035725 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055064 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055081 GO:0055083 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097708 GO:0098655 GO:0098656 GO:0098660 GO:0098661 GO:0098662 GO:0098771 GO:0098791 GO:1902476

KEGG Pathways

Metabolic & Signaling

ko04966 map04966

Pfam Domains

Protein Families

AA_permease SLC12

Protein Analysis

915

Amino Acids

99.57

Weight (kDa)

5.93

Isoelectric Point (pI)

33.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease	PF00324	146 - 609	1.4e-52	Amino acid permease
AA_permease_2	PF13520	168 - 590	2e-14	Amino acid permease
SLC12	PF03522	651 - 772	9.9e-13	Solute carrier family 12
SLC12	PF03522	781 - 982	1.8e-26	Solute carrier family 12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30450 AT1G30450 AT1G30450 AT1G30450
fragaria_vesca	FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910
malus_domestica	MD05G1275000.v1.1 MD10G1254500.v1.1
prunus_persica	Prupe.4G088900_v2.0.a1 Prupe.4G088900_v2.0.a1
pyrus_communis	pycom05g25640 pycom10g21010
rosa_chinensis	RchiOBHm_Chr5g0015611 RchiOBHm_Chr5g0015801 RchiOBHm_Chr5g0015811
rosa_laevigata	RLG00000032213 RLG00000032223
rosa_multiflora	Rmu_co8164906.1_g000001 Rmu_sc0003989.1_g000002 Rmu_sc0005803.1_g000020 Rmu_sc0007629.1_g000001 Rmu_sc0011313.1_g000001 Rmu_sc0011313.1_g000002 Rmu_sc0016973.1_g000001
rosa_roxburghii	Rroxscaffold_1G00060860
rosa_rugosa	Rorug05G0028900
rosa_samantha	Rh5AG122200 Rh5BG120800 Rh5CG131200 Rh5CG132900 Rh5CG133000 Rh5DG119800 Rh5DG120900
rosa_wichuraiana	Rw5G010540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1520
AatII	GACGTC	1 cut(s)	18
AccB7I	CCANNNNNTGG	3 cut(s)	653, 755, 2164
AccBSI	CCGCTC	1 cut(s)	155
AccI	GTMKAC	1 cut(s)	78
AccII	CGCG	2 cut(s)	93, 2148
AclWI	GGATC	6 cut(s)	113, 126, 709, 1061, 1542, 1617
AcsI	RAATTY	3 cut(s)	1646, 2241, 2445
AcuI	CTGAAG	2 cut(s)	210, 2157
AcyI	GRCGYC	3 cut(s)	15, 1633, 2221
AfaI	GTAC	7 cut(s)	65, 345, 421, 553, 2087, 2353, 2737
AfiI	CCNNNNNNNGG	9 cut(s)	73, 362, 653, 755, 982, 983, 1912, 2164, 2422
AflII	CTTAAG	1 cut(s)	314
AflIII	ACRYGT	1 cut(s)	548
AjnI	CCWGG	2 cut(s)	748, 1085
Alw21I	GWGCWC	3 cut(s)	188, 1393, 1869
Alw26I	GTCTC	6 cut(s)	24, 150, 1115, 1737, 2318, 2393
Alw44I	GTGCAC	1 cut(s)	1865
AlwI	GGATC	6 cut(s)	113, 126, 709, 1061, 1542, 1617
AlwNI	CAGNNNCTG	1 cut(s)	755
Ama87I	CYCGRG	1 cut(s)	957
AoxI	GGCC	8 cut(s)	21, 46, 55, 221, 250, 1316, 1907, 2324
ApaI	GGGCCC	1 cut(s)	225
ApaLI	GTGCAC	1 cut(s)	1865
ApeKI	GCWGC	8 cut(s)	1205, 1367, 1406, 1921, 2054, 2065, 2624, 2727
ApoI	RAATTY	3 cut(s)	1646, 2241, 2445
ArsI	GACNNNNNNTTYG	2 cut(s)	2152, 2184
AseI	ATTAAT	2 cut(s)	213, 888
Asp700I	GAANNNNTTC	1 cut(s)	2142
AspA2I	CCTAGG	1 cut(s)	1637
AspLEI	GCGC	3 cut(s)	95, 640, 755
AspS9I	GGNCC	7 cut(s)	221, 222, 251, 614, 644, 1182, 2325
AsuHPI	GGTGA	3 cut(s)	377, 1808, 2033
AsuII	TTCGAA	1 cut(s)	2527
AsuNHI	GCTAGC	1 cut(s)	1825
AvaI	CYCGRG	1 cut(s)	957
AvaII	GGWCC	3 cut(s)	614, 644, 1182
AvrII	CCTAGG	1 cut(s)	1637
AxyI	CCTNAGG	1 cut(s)	1360
BaeGI	GKGCMC	2 cut(s)	225, 1869
BamHI	GGATCC	1 cut(s)	118
BanII	GRGCYC	4 cut(s)	188, 225, 2493, 2593
BbsI	GAAGAC	3 cut(s)	123, 1049, 1832
Bbv12I	GWGCWC	3 cut(s)	188, 1393, 1869
BbvI	GCAGC	8 cut(s)	1192, 1354, 1418, 1908, 2041, 2077, 2611, 2714
BceAI	ACGGC	4 cut(s)	80, 265, 802, 1109
BcgI	CGANNNNNNTGC	2 cut(s)	330, 364
BciT130I	CCWGG	2 cut(s)	750, 1087
BciVI	GTATCC	4 cut(s)	384, 1000, 1893, 2217
BcoDI	GTCTC	6 cut(s)	24, 150, 1115, 1737, 2318, 2393
BfaI	CTAG	8 cut(s)	231, 357, 683, 1616, 1638, 1736, 1826, 2015
BfmI	CTRYAG	1 cut(s)	2728
BfoI	RGCGCY	1 cut(s)	756
BfrI	CTTAAG	1 cut(s)	314
BfuI	GTATCC	4 cut(s)	384, 1000, 1893, 2217
BglI	GCCNNNNNGGC	1 cut(s)	54
BlnI	CCTAGG	1 cut(s)	1637
Bme1390I	CCNGG	2 cut(s)	750, 1087
Bme18I	GGWCC	3 cut(s)	614, 644, 1182
BmeT110I	CYCGRG	1 cut(s)	957
BmgT120I	GGNCC	7 cut(s)	221, 222, 251, 614, 644, 1182, 2325
BmiI	GGNNCC	6 cut(s)	120, 223, 253, 1258, 1661, 2592
BmrFI	CCNGG	2 cut(s)	750, 1087
BmrI	ACTGGG	2 cut(s)	1801, 2121
BmsI	GCATC	7 cut(s)	921, 948, 1609, 1779, 2035, 2062, 2470
BmtI	GCTAGC	1 cut(s)	1829
BmuI	ACTGGG	2 cut(s)	1801, 2121
BpiI	GAAGAC	3 cut(s)	123, 1049, 1832
BplI	GAGNNNNNCTC	4 cut(s)	1456, 1488, 1651, 1683
Bpu14I	TTCGAA	1 cut(s)	2527
BpuEI	CTTGAG	6 cut(s)	122, 152, 2165, 2477, 2580, 2702
Bsa29I	ATCGAT	1 cut(s)	1153
BsaAI	YACGTR	1 cut(s)	1339
BsaHI	GRCGYC	3 cut(s)	15, 1633, 2221
BsaI	GGTCTC	3 cut(s)	1115, 2318, 2393
BsaJI	CCNNGG	9 cut(s)	43, 72, 114, 748, 1085, 1086, 1637, 1910, 2049
BsaXI	ACNNNNNCTCC	2 cut(s)	1375, 1405
Bsc4I	CCNNNNNNNGG	9 cut(s)	73, 362, 653, 755, 982, 983, 1912, 2164, 2422
Bse118I	RCCGGY	2 cut(s)	23, 1905
Bse1I	ACTGG	4 cut(s)	568, 1390, 1796, 2127
Bse21I	CCTNAGG	1 cut(s)	1360
Bse3DI	GCAATG	4 cut(s)	582, 1107, 1260, 2460
BseBI	CCWGG	2 cut(s)	750, 1087
BseCI	ATCGAT	1 cut(s)	1153
BseDI	CCNNGG	9 cut(s)	43, 72, 114, 748, 1085, 1086, 1637, 1910, 2049
BseGI	GGATG	6 cut(s)	148, 477, 846, 1887, 1942, 2707
BseLI	CCNNNNNNNGG	9 cut(s)	73, 362, 653, 755, 982, 983, 1912, 2164, 2422
BseMI	GCAATG	4 cut(s)	582, 1107, 1260, 2460
BseMII	CTCAG	4 cut(s)	398, 1190, 2100, 2646
BseNI	ACTGG	4 cut(s)	568, 1390, 1796, 2127
BseRI	GAGGAG	4 cut(s)	2402, 2405, 2483, 2583
BseSI	GKGCMC	2 cut(s)	225, 1869
BseXI	GCAGC	8 cut(s)	1192, 1354, 1418, 1908, 2041, 2077, 2611, 2714
BseYI	CCCAGC	5 cut(s)	755, 1324, 1625, 1784, 1849
Bsh1236I	CGCG	2 cut(s)	93, 2148
Bsh1285I	CGRYCG	2 cut(s)	91, 1847
BshFI	GGCC	8 cut(s)	23, 48, 57, 223, 252, 1318, 1909, 2326
BshVI	ATCGAT	1 cut(s)	1153
BsiEI	CGRYCG	2 cut(s)	91, 1847
BsiHKAI	GWGCWC	3 cut(s)	188, 1393, 1869
BsiHKCI	CYCGRG	1 cut(s)	957
BsiSI	CCGG	3 cut(s)	24, 49, 1906
BslFI	GGGAC	7 cut(s)	425, 731, 999, 1067, 1103, 1802, 2698
BslI	CCNNNNNNNGG	9 cut(s)	73, 362, 653, 755, 982, 983, 1912, 2164, 2422
BsmAI	GTCTC	6 cut(s)	24, 150, 1115, 1737, 2318, 2393
BsmBI	CGTCTC	2 cut(s)	24, 150
BsmFI	GGGAC	7 cut(s)	425, 731, 999, 1067, 1103, 1802, 2698
BsmI	GAATGC	5 cut(s)	905, 1059, 1328, 1496, 2535
BsnI	GGCC	8 cut(s)	23, 48, 57, 223, 252, 1318, 1909, 2326
Bso31I	GGTCTC	3 cut(s)	1115, 2318, 2393
BsoBI	CYCGRG	1 cut(s)	957
Bsp119I	TTCGAA	1 cut(s)	2527
Bsp120I	GGGCCC	1 cut(s)	221
Bsp1286I	GDGCHC	6 cut(s)	188, 225, 1393, 1869, 2493, 2593
Bsp19I	CCATGG	1 cut(s)	114
BspANI	GGCC	8 cut(s)	23, 48, 57, 223, 252, 1318, 1909, 2326
BspCNI	CTCAG	4 cut(s)	397, 1189, 2101, 2647
BspDI	ATCGAT	1 cut(s)	1153
BspFNI	CGCG	2 cut(s)	93, 2148
BspLI	GGNNCC	6 cut(s)	120, 223, 253, 1258, 1661, 2592
BspMAI	CTGCAG	1 cut(s)	2732
BspOI	GCTAGC	1 cut(s)	1829
BspPI	GGATC	6 cut(s)	113, 126, 709, 1061, 1542, 1617
BspQI	GCTCTTC	1 cut(s)	1377
BspT104I	TTCGAA	1 cut(s)	2527
BspTI	CTTAAG	1 cut(s)	314
BspTNI	GGTCTC	3 cut(s)	1115, 2318, 2393
BsrBI	CCGCTC	1 cut(s)	155
BsrDI	GCAATG	4 cut(s)	582, 1107, 1260, 2460
BsrFI	RCCGGY	2 cut(s)	23, 1905
BsrI	ACTGG	4 cut(s)	568, 1390, 1796, 2127
BssAI	RCCGGY	2 cut(s)	23, 1905
BssECI	CCNNGG	9 cut(s)	43, 72, 114, 748, 1085, 1086, 1637, 1910, 2049
BssNI	GRCGYC	3 cut(s)	15, 1633, 2221
BssT1I	CCWWGG	5 cut(s)	43, 114, 1637, 1910, 2049
Bst2UI	CCWGG	2 cut(s)	750, 1087
Bst4CI	ACNGT	8 cut(s)	348, 525, 769, 781, 1726, 1970, 2021, 2096
Bst6I	CTCTTC	2 cut(s)	1377, 1674
BstACI	GRCGYC	3 cut(s)	15, 1633, 2221
BstAFI	CTTAAG	1 cut(s)	314
BstAPI	GCANNNNNTGC	1 cut(s)	820
BstBAI	YACGTR	1 cut(s)	1339
BstBI	TTCGAA	1 cut(s)	2527
BstDEI	CTNAG	7 cut(s)	384, 1176, 1360, 2109, 2421, 2655, 2673
BstDSI	CCRYGG	2 cut(s)	72, 114
BstENI	CCTNNNNNAGG	1 cut(s)	2420
BstF5I	GGATG	6 cut(s)	148, 477, 846, 1887, 1942, 2707
BstFNI	CGCG	2 cut(s)	93, 2148
BstH2I	RGCGCY	1 cut(s)	756
BstHHI	GCGC	3 cut(s)	95, 640, 755
BstMAI	GTCTC	6 cut(s)	24, 150, 1115, 1737, 2318, 2393
BstMCI	CGRYCG	2 cut(s)	91, 1847
BstMWI	GCNNNNNNNGC	9 cut(s)	54, 66, 512, 820, 1412, 1961, 2054, 2291, 2621
BstNI	CCWGG	2 cut(s)	750, 1087
BstNSI	RCATGY	2 cut(s)	552, 2537
BstSCI	CCNGG	2 cut(s)	748, 1085
BstSFI	CTRYAG	1 cut(s)	2728
BstSLI	GKGCMC	2 cut(s)	225, 1869
BstSNI	TACGTA	1 cut(s)	1339
BstUI	CGCG	2 cut(s)	93, 2148
BstV1I	GCAGC	8 cut(s)	1192, 1354, 1418, 1908, 2041, 2077, 2611, 2714
BstV2I	GAAGAC	3 cut(s)	123, 1049, 1832
BstX2I	RGATCY	4 cut(s)	118, 701, 1534, 1609
BstXI	CCANNNNNNTGG	1 cut(s)	1878
BstYI	RGATCY	4 cut(s)	118, 701, 1534, 1609
Bsu15I	ATCGAT	1 cut(s)	1153
Bsu36I	CCTNAGG	1 cut(s)	1360
BsuI	GTATCC	4 cut(s)	384, 1000, 1893, 2217
BsuRI	GGCC	8 cut(s)	23, 48, 57, 223, 252, 1318, 1909, 2326
BsuTUI	ATCGAT	1 cut(s)	1153
BtgI	CCRYGG	2 cut(s)	72, 114
BtsCI	GGATG	6 cut(s)	148, 477, 846, 1887, 1942, 2707
BtsIMutI	CAGTG	5 cut(s)	521, 765, 1163, 2092, 2475
CaiI	CAGNNNCTG	1 cut(s)	755
CfoI	GCGC	3 cut(s)	95, 640, 755
Cfr10I	RCCGGY	2 cut(s)	23, 1905
Cfr13I	GGNCC	7 cut(s)	221, 222, 251, 614, 644, 1182, 2325
ClaI	ATCGAT	1 cut(s)	1153
CseI	GACGC	3 cut(s)	1061, 1622, 2210
Csp6I	GTAC	7 cut(s)	64, 344, 420, 552, 2086, 2352, 2736
CspCI	CAANNNNNGTGG	2 cut(s)	2175, 2210
CviQI	GTAC	7 cut(s)	64, 344, 420, 552, 2086, 2352, 2736
DdeI	CTNAG	7 cut(s)	384, 1176, 1360, 2109, 2421, 2655, 2673
Eam1104I	CTCTTC	2 cut(s)	1377, 1674
EarI	CTCTTC	2 cut(s)	1377, 1674
EciI	GGCGGA	2 cut(s)	42, 2714
Ecl136II	GAGCTC	1 cut(s)	186
Eco105I	TACGTA	1 cut(s)	1339
Eco130I	CCWWGG	5 cut(s)	43, 114, 1637, 1910, 2049
Eco24I	GRGCYC	4 cut(s)	188, 225, 2493, 2593
Eco31I	GGTCTC	3 cut(s)	1115, 2318, 2393
Eco32I	GATATC	1 cut(s)	1426
Eco47I	GGWCC	3 cut(s)	614, 644, 1182
Eco53kI	GAGCTC	1 cut(s)	186
Eco57I	CTGAAG	2 cut(s)	210, 2157
Eco81I	CCTNAGG	1 cut(s)	1360
Eco88I	CYCGRG	1 cut(s)	957
EcoICRI	GAGCTC	1 cut(s)	186
EcoNI	CCTNNNNNAGG	1 cut(s)	2420
EcoO109I	RGGNCCY	2 cut(s)	221, 222
EcoRII	CCWGG	2 cut(s)	748, 1085
EcoRV	GATATC	1 cut(s)	1426
EcoT14I	CCWWGG	5 cut(s)	43, 114, 1637, 1910, 2049
EcoT22I	ATGCAT	1 cut(s)	2535
EcoT38I	GRGCYC	4 cut(s)	188, 225, 2493, 2593
ErhI	CCWWGG	5 cut(s)	43, 114, 1637, 1910, 2049
Esp3I	CGTCTC	2 cut(s)	24, 150
FalI	AAGNNNNNCTT	4 cut(s)	183, 215, 2414, 2446
FaqI	GGGAC	7 cut(s)	425, 731, 999, 1067, 1103, 1802, 2698
FauI	CCCGC	3 cut(s)	160, 910, 1573
FblI	GTMKAC	1 cut(s)	78
FokI	GGATG	6 cut(s)	135, 464, 833, 1874, 1929, 2714
FriOI	GRGCYC	4 cut(s)	188, 225, 2493, 2593
FspBI	CTAG	8 cut(s)	231, 357, 683, 1616, 1638, 1736, 1826, 2015
GlaI	GCGC	3 cut(s)	94, 639, 754
GsaI	CCCAGC	5 cut(s)	759, 1328, 1629, 1788, 1853
HaeII	RGCGCY	1 cut(s)	756
HaeIII	GGCC	8 cut(s)	23, 48, 57, 223, 252, 1318, 1909, 2326
HapII	CCGG	3 cut(s)	24, 49, 1906
HgaI	GACGC	3 cut(s)	1061, 1622, 2210
HhaI	GCGC	3 cut(s)	95, 640, 755
Hin1I	GRCGYC	3 cut(s)	15, 1633, 2221
Hin6I	GCGC	3 cut(s)	93, 638, 753
HinP1I	GCGC	3 cut(s)	93, 638, 753
HincII	GTYRAC	3 cut(s)	79, 784, 2667
HindII	GTYRAC	3 cut(s)	79, 784, 2667
HindIII	AAGCTT	1 cut(s)	2428
HinfI	GANTC	8 cut(s)	263, 290, 352, 518, 987, 1528, 2603, 2686
HpaI	GTTAAC	1 cut(s)	784
HpaII	CCGG	3 cut(s)	24, 49, 1906
HphI	GGTGA	3 cut(s)	377, 1808, 2033
Hpy166II	GTNNAC	7 cut(s)	79, 614, 784, 1469, 1588, 1867, 2667
Hpy188I	TCNGA	6 cut(s)	190, 1041, 1835, 2137, 2247, 2340
Hpy8I	GTNNAC	7 cut(s)	79, 614, 784, 1469, 1588, 1867, 2667
HpyAV	CCTTC	9 cut(s)	132, 212, 598, 742, 1577, 1613, 1975, 2093, 2132
HpyCH4III	ACNGT	8 cut(s)	348, 525, 769, 781, 1726, 1970, 2021, 2096
HpyCH4IV	ACGT	2 cut(s)	15, 1338
HpyF10VI	GCNNNNNNNGC	9 cut(s)	54, 66, 512, 820, 1412, 1961, 2054, 2291, 2621
HpyF3I	CTNAG	7 cut(s)	384, 1176, 1360, 2109, 2421, 2655, 2673
HpySE526I	ACGT	2 cut(s)	15, 1338
Hsp92I	GRCGYC	3 cut(s)	15, 1633, 2221
HspAI	GCGC	3 cut(s)	93, 638, 753
KroI	GCCGGC	2 cut(s)	23, 1905
KroNI	GCCGGC	2 cut(s)	25, 1907
KspAI	GTTAAC	1 cut(s)	784
LguI	GCTCTTC	1 cut(s)	1377
LmnI	GCTCC	8 cut(s)	719, 1256, 1396, 1627, 1659, 1676, 1853, 2596
Lsp1109I	GCAGC	8 cut(s)	1192, 1354, 1418, 1908, 2041, 2077, 2611, 2714
LweI	GCATC	7 cut(s)	921, 948, 1609, 1779, 2035, 2062, 2470
MaeI	CTAG	8 cut(s)	231, 357, 683, 1616, 1638, 1736, 1826, 2015
MaeII	ACGT	2 cut(s)	15, 1338
MaeIII	GTNAC	5 cut(s)	532, 769, 841, 1126, 2551
MbiI	CCGCTC	1 cut(s)	155
MflI	RGATCY	4 cut(s)	118, 701, 1534, 1609
MhlI	GDGCHC	6 cut(s)	188, 225, 1393, 1869, 2493, 2593
MlyI	GAGTC	3 cut(s)	361, 981, 2680
MmeI	TCCRAC	2 cut(s)	270, 634
Mph1103I	ATGCAT	1 cut(s)	2535
MroNI	GCCGGC	2 cut(s)	23, 1905
MroXI	GAANNNNTTC	1 cut(s)	2142
MseI	TTAA	6 cut(s)	213, 315, 783, 888, 1440, 2234
MspA1I	CMGCKG	3 cut(s)	1180, 1629, 2692
MspCI	CTTAAG	1 cut(s)	314
MspI	CCGG	3 cut(s)	24, 49, 1906
MspR9I	CCNGG	2 cut(s)	750, 1087
Mva1269I	GAATGC	5 cut(s)	905, 1059, 1328, 1496, 2535
MvaI	CCWGG	2 cut(s)	750, 1087
MvnI	CGCG	2 cut(s)	93, 2148
MwoI	GCNNNNNNNGC	9 cut(s)	54, 66, 512, 820, 1412, 1961, 2054, 2291, 2621
NaeI	GCCGGC	2 cut(s)	25, 1907
NcoI	CCATGG	1 cut(s)	114
NgoMIV	GCCGGC	2 cut(s)	23, 1905
NheI	GCTAGC	1 cut(s)	1825
NlaIV	GGNNCC	6 cut(s)	120, 223, 253, 1258, 1661, 2592
NmuCI	GTSAC	2 cut(s)	841, 1126
NsiI	ATGCAT	1 cut(s)	2535
NspI	RCATGY	2 cut(s)	552, 2537
NspV	TTCGAA	1 cut(s)	2527
PaeI	GCATGC	1 cut(s)	2537
PasI	CCCWGGG	1 cut(s)	1086
PciI	ACATGT	1 cut(s)	548
PciSI	GCTCTTC	1 cut(s)	1377
PcsI	WCGNNNNNNNCGW	2 cut(s)	84, 93
PctI	GAATGC	5 cut(s)	905, 1059, 1328, 1496, 2535
PdiI	GCCGGC	2 cut(s)	25, 1907
PdmI	GAANNNNTTC	1 cut(s)	2142
PfeI	GAWTC	5 cut(s)	263, 290, 518, 1528, 2603
PflMI	CCANNNNNTGG	3 cut(s)	653, 755, 2164
Ple19I	CGATCG	2 cut(s)	91, 1847
PleI	GAGTC	3 cut(s)	360, 981, 2680
PpsI	GAGTC	3 cut(s)	360, 981, 2680
Ppu21I	YACGTR	1 cut(s)	1339
PscI	ACATGT	1 cut(s)	548
PshBI	ATTAAT	2 cut(s)	213, 888
PsiI	TTATAA	1 cut(s)	1520
Psp124BI	GAGCTC	1 cut(s)	188
Psp6I	CCWGG	2 cut(s)	748, 1085
PspFI	CCCAGC	5 cut(s)	755, 1324, 1625, 1784, 1849
PspGI	CCWGG	2 cut(s)	748, 1085
PspN4I	GGNNCC	6 cut(s)	120, 223, 253, 1258, 1661, 2592
PspOMI	GGGCCC	1 cut(s)	221
PspPI	GGNCC	7 cut(s)	221, 222, 251, 614, 644, 1182, 2325
PstI	CTGCAG	1 cut(s)	2732
PstNI	CAGNNNCTG	1 cut(s)	755
PsuI	RGATCY	4 cut(s)	118, 701, 1534, 1609
PvuI	CGATCG	2 cut(s)	91, 1847
PvuII	CAGCTG	1 cut(s)	1629
RsaI	GTAC	7 cut(s)	65, 345, 421, 553, 2087, 2353, 2737
RsaNI	GTAC	7 cut(s)	64, 344, 420, 552, 2086, 2352, 2736
SacI	GAGCTC	1 cut(s)	188
SalI	GTCGAC	1 cut(s)	77
SapI	GCTCTTC	1 cut(s)	1377
SaqAI	TTAA	6 cut(s)	213, 315, 783, 888, 1440, 2234
Sau96I	GGNCC	7 cut(s)	221, 222, 251, 614, 644, 1182, 2325
SchI	GAGTC	3 cut(s)	361, 981, 2680
ScrFI	CCNGG	2 cut(s)	750, 1087
SduI	GDGCHC	6 cut(s)	188, 225, 1393, 1869, 2493, 2593
SfaNI	GCATC	7 cut(s)	921, 948, 1609, 1779, 2035, 2062, 2470
SfcI	CTRYAG	1 cut(s)	2728
SfiI	GGCCNNNNNGGCC	1 cut(s)	54
SfuI	TTCGAA	1 cut(s)	2527
SinI	GGWCC	3 cut(s)	614, 644, 1182
SmlI	CTYRAG	7 cut(s)	101, 167, 314, 2180, 2492, 2595, 2717
SmoI	CTYRAG	7 cut(s)	101, 167, 314, 2180, 2492, 2595, 2717
SnaBI	TACGTA	1 cut(s)	1339
SphI	GCATGC	1 cut(s)	2537
SspI	AATATT	1 cut(s)	304
SspMI	CTAG	8 cut(s)	231, 357, 683, 1616, 1638, 1736, 1826, 2015
SstI	GAGCTC	1 cut(s)	188
StyD4I	CCNGG	2 cut(s)	748, 1085
StyI	CCWWGG	5 cut(s)	43, 114, 1637, 1910, 2049
TaaI	ACNGT	8 cut(s)	348, 525, 769, 781, 1726, 1970, 2021, 2096
TaiI	ACGT	2 cut(s)	18, 1341
TaqI	TCGA	8 cut(s)	18, 78, 350, 929, 1153, 1843, 2316, 2527
TaqII	GACCGA	3 cut(s)	299, 1097, 1301
TatI	WGTACW	3 cut(s)	551, 2085, 2735
TauI	GCSGC	3 cut(s)	60, 341, 2151
TfiI	GAWTC	5 cut(s)	263, 290, 518, 1528, 2603
Tru1I	TTAA	6 cut(s)	213, 315, 783, 888, 1440, 2234
Tru9I	TTAA	6 cut(s)	213, 315, 783, 888, 1440, 2234
TscAI	CASTG	5 cut(s)	528, 772, 1170, 2099, 2482
TseFI	GTSAC	2 cut(s)	841, 1126
TseI	GCWGC	8 cut(s)	1205, 1367, 1406, 1921, 2054, 2065, 2624, 2727
Tsp45I	GTSAC	2 cut(s)	841, 1126
TspDTI	ATGAA	8 cut(s)	231, 617, 806, 1321, 1988, 2573, 2616, 2675
TspGWI	ACGGA	2 cut(s)	470, 1231
TspRI	CASTG	5 cut(s)	528, 772, 1170, 2099, 2482
Van91I	CCANNNNNTGG	3 cut(s)	653, 755, 2164
Vha464I	CTTAAG	1 cut(s)	314
VneI	GTGCAC	1 cut(s)	1865
VpaK11BI	GGWCC	3 cut(s)	614, 644, 1182
VspI	ATTAAT	2 cut(s)	213, 888
XagI	CCTNNNNNAGG	1 cut(s)	2420
XapI	RAATTY	3 cut(s)	1646, 2241, 2445
XceI	RCATGY	2 cut(s)	552, 2537
XmaJI	CCTAGG	1 cut(s)	1637
XmiI	GTMKAC	1 cut(s)	78
XmnI	GAANNNNTTC	1 cut(s)	2142
XspI	CTAG	8 cut(s)	231, 357, 683, 1616, 1638, 1736, 1826, 2015
ZraI	GACGTC	1 cut(s)	16
Zsp2I	ATGCAT	1 cut(s)	2535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.