Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0003023

Physical Location & Seq

Genomic Coordinates Reverse (-)

83076469 .. 83086213

Gene Structure 9745 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00060860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2946 bp

ATGGACAATGCGGACGTCGAAGCCGGCGGAGACGACGAGTTCCAAGGCCGGAGGGGCCGCAAGTATCGCCCTGTGGTCGGCGACGACGATCGCGCTGTGCTTGAGATGTCGTCCATCGATCCTTCGTCTTCGTCTTCTTCCTCTCAGCCCGCTTACCAATCTTCGCTCAAGAACATAAAAGTGAGCTCTGAGGAGAACCTTGGCTCTAACATAAAAGAAGGGTCCTCTGTTAGTCATGTGCAAGCCAACGGCCCCCAGAAAGAATCCAAATTGGAACTCTTTGGCTTTGATTCTCTTGTCAATATTCTTGGTCTTAAGAGTATGACAGATGAGCAAGCTGCAGCACCATCTAGTCCTAGAGATGGTGAAAATATTGCTATCACCCGTGGAAGACCGAAGGATACTGTCGTCAAATCGGGTACATTGATGGGTGTATTTGTCCCATGCCTGCAAAACATATTGGGAATTATCTACTACATCCGTTTTACTTGGATCGTTGGTATGGCTGGCATAGCTGATTCACTGTTAGTGGTTACATTATGTGGCACATGTACTTTCCTGACTGGAATATCATTGAGTGCCATTGCAACCAATGGTGCTATGAAGGGAGGTGGACCATACTATCTAATTGGTCGTGCGCTTGGTCCAGAAGTTGGAGTTAGCATTGGGTTATGTTTCTTTCTTGGAAATGCTGTTGCTGGAGCTCTTTATGTCTTGGGAGCTGTGGAAACCTTCTTGAAAGCTCTGCCAGGCGCTGGGTTTTTCAGTGAAACAACAACAGTTAATGACTTAAAGACTTTTAATCTGAGTATGCATGATTTGCAAATCTATGGGATAATTGTGACCATCCTATTGTGCTTTATTGTGTTTGGGGGAGTGAAAATAATCAATAAGGTTGCACCTGCTTTCCTCATACCCGTTCTATTGTCAATGCTCTGCATCTATATCGGGATTGCTCTGGCAAGGAAGGATTACCCTACAAAGGGAGTCACGGGCTTTAGTTTTAATACGTTCAAAGATAACTGGAAATCGGATTATCAGAAGACTACTAGCGGTGGAGTTCCTGACCCTGATGGAGCTGTCTCCTGGGACTTCAATGCAATGGTCGGTCTCTTTTTCCCTGCCGTGACAGGAATTATGGCAGGTTCAAATCGATCAGCCTCACTGAAAGACACTCAGCGGTCAATTCCTATTGGAACATTGGCTGCAACTCTTGCAACTACTGTTATGTACCTGATCTCTGTGTTAGTATTTGGAGGACTTGCCACCAGACATTTGCTTTTGACTGACAGGCTACTTTCGGCTACTATATCTTGGCCTTTCCCAGCATTCATTTACGTGGGAATAATTCTTTCAACCTTAGGTGCTGCTCTTCAAAGCCTGACTGGTGCTCCCCGTCTGCTTGCTGCAATAGCCAATGATGATATCTTACCAGTTCTTAACTACTTCAAGGTTGCAGATGGGAGTGAACCTCACTTTGCTACCTTATTTACCGCATTCCTCTGTATTGCGTGTGTTATAATTGGGAACCTGGATCTTATCACACCAACTATAACTATGTTTTTCCTTCTGTGTTATGCCGGTGTGAACTTATCTTGCTTCCTTCTGGATCTCCTAGATGCTCCCAGCTGGCGTCCTCGGTGGAAATTCCACCATTGGAGCCTCTCTCTTCTTGGAGCTTCACTATGTTTAGTGATTATGTTCTTGATCTCTTGGGCATTCACTATAGTGTCGCTCGCCCTTGCAAGCCTTATATATTATTATGTGAGCATCAAAGGGAAGGCTGGGGACTGGGGTGATGGTTTCAAGAGCGCATATTTTCAGCTAGCTCTCCGAAGTCTTCGATCGCTAGGAGCAAACCAAGTGCACCCAAAGAATTGGTATCCCATCCCCCTGATATTCTGCCGGCCTTGGGGGAAGCTGCCAGAGAATGTACCCTGCCATCCCAAACTTGCCGACTTCGCCAACTGCATGAAGAAGAAGGGCAGGGGAATGTCTATCTTTGTGTCTATTCTAGATGGTGATTACCATGAATGTGTTGAAGATGCCAAGGCTGCCTGCAAGCAGCTTGATGCTTACATTGAGTACAAGCACTGTGAAGGTGTAGCTGAGATTGTTGTCGCCCCCAGTATGTCTGAAGGCTTCCGCGGCATTGTTCAGACAATGGGTCTTGGCAATCTCAAGCCAAACATAGTGGTGATGCGGTATCCTGAAATATGGCGTCGCGAAAACTTAACAGAAATTCCAAACACCTTTGTTGAAATAATAAATGATTGCATAGTTGCAAACAAGGCAGTAGTTATTGTCAAAGGTCTTGACGAATGGCCCAATGAGTTTCAGAGGCAGTATGGTACTATTGATCTCTATTGGATAGTAAGAGATGGAGGTCTGATGCTTCTTCTCTCTCAGCTCCTCCTCACTAAGGAAAGCTTTGAAAGTTGTAAAATTCAAGTTTTCTGCATTGCGGAGGAGGATACTGATGCAGAGGGGCTCAAGGCTGATGTGAAGAAGTTTCTGTATGATCTTCGAATGCATGCAGAAGTCATTGTTGTAACAATGAAGTCGTGGGATATGCAAGCAGACAGTGGGGCTCCTCAAGATGAATCTGTGGAGGCTTATAATGCTGCTAAAAAGCGGATAGCTGATTACTTGGCTGAGATGAAGTCAACAGCTAAGAAACAAGGGACACCACTGATGGCGGATGGGAAACAGGTCGTTGTGAATGAGCAGCAGGTGGAGAAGTTTCTTTACACTACTCTGAAGTTGAATTCAACAATACTCAGATACTCGAGAATGGCTGCGGTCGTGCTCGTTAGCTTACCTCCTCCTCCAGTCAACCACCCAGCATACTTTTACATGGAATACATGGATTTGTTGGTGGAGAATGTGCCTAGACTTCTGATTGTGAGAGGATACCGTAGGGATGTTGTAACTCTATTCACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006820 GO:0006821 GO:0008150 GO:0008324 GO:0008509 GO:0008511 GO:0009674 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015103 GO:0015108 GO:0015291 GO:0015293 GO:0015294 GO:0015296 GO:0015318 GO:0015370 GO:0015373 GO:0015377 GO:0015378 GO:0015379 GO:0015672 GO:0015698 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0034220 GO:0035725 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055064 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055081 GO:0055083 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097708 GO:0098655 GO:0098656 GO:0098660 GO:0098661 GO:0098662 GO:0098771 GO:0098791 GO:1902476

KEGG Pathways

Metabolic & Signaling

ko04966 map04966

Pfam Domains

Protein Families

AA_permease SLC12

Protein Analysis

981

Amino Acids

107.5

Weight (kDa)

6.36

Isoelectric Point (pI)

37.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease	PF00324	144 - 607	1e-54	Amino acid permease
AA_permease_2	PF13520	165 - 588	1.2e-15	Amino acid permease
SLC12	PF03522	649 - 770	5.4e-13	Solute carrier family 12
SLC12	PF03522	779 - 980	2.1e-26	Solute carrier family 12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30450 AT1G30450 AT1G30450 AT1G30450
fragaria_vesca	FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910
malus_domestica	MD05G1275000.v1.1 MD10G1254500.v1.1
prunus_persica	Prupe.4G088900_v2.0.a1 Prupe.4G088900_v2.0.a1
pyrus_communis	pycom05g25640 pycom10g21010
rosa_chinensis	RchiOBHm_Chr5g0015611 RchiOBHm_Chr5g0015801 RchiOBHm_Chr5g0015811
rosa_laevigata	RLG00000032213 RLG00000032223
rosa_multiflora	Rmu_co8164906.1_g000001 Rmu_sc0003989.1_g000002 Rmu_sc0005803.1_g000020 Rmu_sc0007629.1_g000001 Rmu_sc0011313.1_g000001 Rmu_sc0011313.1_g000002 Rmu_sc0016973.1_g000001
rosa_roxburghii	Rroxscaffold_1G00060860
rosa_rugosa	Rorug05G0028900
rosa_samantha	Rh5AG122200 Rh5BG120800 Rh5CG131200 Rh5CG132900 Rh5CG133000 Rh5DG119800 Rh5DG120900
rosa_wichuraiana	Rw5G010540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1520, 2619
AarI	CACCTGC	2 cut(s)	910, 2722
AatII	GACGTC	1 cut(s)	18
Acc36I	ACCTGC	3 cut(s)	910, 1133, 2722
AccB7I	CCANNNNNTGG	2 cut(s)	653, 755
AccII	CGCG	3 cut(s)	93, 2148, 2226
AclWI	GGATC	4 cut(s)	113, 500, 1542, 1617
AcsI	RAATTY	4 cut(s)	1646, 2241, 2445, 2767
AcuI	CTGAAG	2 cut(s)	2157, 2780
AcyI	GRCGYC	3 cut(s)	15, 1633, 2221
AfaI	GTAC	6 cut(s)	421, 553, 1232, 1935, 2087, 2353
AfiI	CCNNNNNNNGG	8 cut(s)	77, 362, 653, 755, 982, 983, 1912, 2422
AflII	CTTAAG	1 cut(s)	314
AflIII	ACRYGT	1 cut(s)	548
AhdI	GACNNNNNGTC	1 cut(s)	2166
AjnI	CCWGG	3 cut(s)	748, 1085, 1530
AleI	CACNNNNGTG	1 cut(s)	1727
Alw21I	GWGCWC	5 cut(s)	188, 706, 1393, 1869, 2811
Alw26I	GTCTC	3 cut(s)	24, 1087, 1115
Alw44I	GTGCAC	1 cut(s)	1865
AlwI	GGATC	4 cut(s)	113, 500, 1542, 1617
AlwNI	CAGNNNCTG	1 cut(s)	755
Ama87I	CYCGRG	1 cut(s)	2788
AoxI	GGCC	6 cut(s)	46, 55, 250, 1316, 1907, 2324
ApaLI	GTGCAC	1 cut(s)	1865
ApoI	RAATTY	4 cut(s)	1646, 2241, 2445, 2767
AspLEI	GCGC	4 cut(s)	95, 640, 755, 1814
AspS9I	GGNCC	6 cut(s)	55, 222, 251, 614, 644, 2325
AsuHPI	GGTGA	5 cut(s)	373, 377, 1808, 2033, 2209
AsuII	TTCGAA	1 cut(s)	2527
AsuNHI	GCTAGC	1 cut(s)	1825
AvaI	CYCGRG	1 cut(s)	2788
AvaII	GGWCC	3 cut(s)	222, 614, 644
AxyI	CCTNAGG	1 cut(s)	1360
BaeGI	GKGCMC	1 cut(s)	1869
BaeI	ACNNNNGTAYC	2 cut(s)	393, 426
BanII	GRGCYC	4 cut(s)	188, 706, 2493, 2593
BarI	GAAGNNNNNNTAC	2 cut(s)	2732, 2764
BbsI	GAAGAC	5 cut(s)	120, 126, 397, 1049, 1832
Bbv12I	GWGCWC	5 cut(s)	188, 706, 1393, 1869, 2811
BceAI	ACGGC	2 cut(s)	265, 1109
BcgI	CGANNNNNNTGC	2 cut(s)	928, 962
BciT130I	CCWGG	3 cut(s)	750, 1087, 1532
BciVI	GTATCC	5 cut(s)	394, 1893, 2217, 2467, 2906
BcoDI	GTCTC	3 cut(s)	24, 1087, 1115
BfaI	CTAG	8 cut(s)	351, 357, 1050, 1616, 1826, 1850, 2015, 2892
BfmI	CTRYAG	2 cut(s)	339, 1725
BfoI	RGCGCY	1 cut(s)	756
BfrI	CTTAAG	1 cut(s)	314
BfuAI	ACCTGC	3 cut(s)	910, 1133, 2722
BfuI	GTATCC	5 cut(s)	394, 1893, 2217, 2467, 2906
BglI	GCCNNNNNGGC	1 cut(s)	54
Bme1390I	CCNGG	3 cut(s)	750, 1087, 1532
Bme18I	GGWCC	3 cut(s)	222, 614, 644
BmeRI	GACNNNNNGTC	1 cut(s)	2166
BmeT110I	CYCGRG	1 cut(s)	2788
BmgT120I	GGNCC	6 cut(s)	55, 222, 251, 614, 644, 2325
BmiI	GGNNCC	6 cut(s)	56, 223, 253, 1529, 1661, 2592
BmrFI	CCNGG	3 cut(s)	750, 1087, 1532
BmrI	ACTGGG	2 cut(s)	1801, 2121
BmsI	GCATC	8 cut(s)	948, 1609, 1779, 2035, 2062, 2190, 2382, 2470
BmtI	GCTAGC	1 cut(s)	1829
BmuI	ACTGGG	2 cut(s)	1801, 2121
BpiI	GAAGAC	5 cut(s)	120, 126, 397, 1049, 1832
BplI	GAGNNNNNCTC	4 cut(s)	1456, 1488, 1651, 1683
BpmI	CTGGAG	2 cut(s)	720, 2814
Bpu14I	TTCGAA	1 cut(s)	2527
BpuEI	CTTGAG	5 cut(s)	122, 152, 2165, 2477, 2580
Bsa29I	ATCGAT	2 cut(s)	117, 1153
BsaAI	YACGTR	2 cut(s)	1339, 2943
BsaHI	GRCGYC	3 cut(s)	15, 1633, 2221
BsaI	GGTCTC	1 cut(s)	1115
BsaJI	CCNNGG	8 cut(s)	43, 199, 385, 1086, 1637, 1910, 2049, 2146
BsaXI	ACNNNNNCTCC	2 cut(s)	1375, 1405
Bsc4I	CCNNNNNNNGG	8 cut(s)	77, 362, 653, 755, 982, 983, 1912, 2422
Bse118I	RCCGGY	3 cut(s)	23, 1580, 1905
Bse1I	ACTGG	7 cut(s)	568, 1028, 1390, 1433, 1796, 2127, 2831
Bse21I	CCTNAGG	1 cut(s)	1360
Bse3DI	GCAATG	3 cut(s)	582, 1107, 2460
BseBI	CCWGG	3 cut(s)	750, 1087, 1532
BseCI	ATCGAT	2 cut(s)	117, 1153
BseDI	CCNNGG	8 cut(s)	43, 199, 385, 1086, 1637, 1910, 2049, 2146
BseGI	GGATG	6 cut(s)	477, 846, 1887, 1942, 2707, 2929
BseLI	CCNNNNNNNGG	8 cut(s)	77, 362, 653, 755, 982, 983, 1912, 2422
BseMI	GCAATG	3 cut(s)	582, 1107, 2460
BseMII	CTCAG	8 cut(s)	158, 180, 797, 1190, 2100, 2420, 2646, 2794
BseNI	ACTGG	7 cut(s)	568, 1028, 1390, 1433, 1796, 2127, 2831
BseRI	GAGGAG	7 cut(s)	206, 2402, 2405, 2483, 2583, 2814, 2817
BseSI	GKGCMC	1 cut(s)	1869
BseYI	CCCAGC	5 cut(s)	755, 1324, 1625, 1784, 2842
Bsh1236I	CGCG	3 cut(s)	93, 2148, 2226
Bsh1285I	CGRYCG	3 cut(s)	91, 1847, 2805
BshFI	GGCC	6 cut(s)	48, 57, 252, 1318, 1909, 2326
BshVI	ATCGAT	2 cut(s)	117, 1153
BsiEI	CGRYCG	3 cut(s)	91, 1847, 2805
BsiHKAI	GWGCWC	5 cut(s)	188, 706, 1393, 1869, 2811
BsiHKCI	CYCGRG	1 cut(s)	2788
BsiSI	CCGG	4 cut(s)	24, 49, 1581, 1906
BslFI	GGGAC	4 cut(s)	425, 1103, 1802, 2698
BslI	CCNNNNNNNGG	8 cut(s)	77, 362, 653, 755, 982, 983, 1912, 2422
BsmAI	GTCTC	3 cut(s)	24, 1087, 1115
BsmBI	CGTCTC	1 cut(s)	24
BsmFI	GGGAC	4 cut(s)	425, 1103, 1802, 2698
BsmI	GAATGC	4 cut(s)	1328, 1496, 1718, 2535
BsnI	GGCC	6 cut(s)	48, 57, 252, 1318, 1909, 2326
Bso31I	GGTCTC	1 cut(s)	1115
BsoBI	CYCGRG	1 cut(s)	2788
Bsp119I	TTCGAA	1 cut(s)	2527
Bsp1286I	GDGCHC	7 cut(s)	188, 706, 1393, 1869, 2493, 2593, 2811
Bsp68I	TCGCGA	1 cut(s)	2226
BspANI	GGCC	6 cut(s)	48, 57, 252, 1318, 1909, 2326
BspCNI	CTCAG	8 cut(s)	157, 181, 798, 1189, 2101, 2419, 2647, 2793
BspDI	ATCGAT	2 cut(s)	117, 1153
BspFNI	CGCG	3 cut(s)	93, 2148, 2226
BspLI	GGNNCC	6 cut(s)	56, 223, 253, 1529, 1661, 2592
BspMAI	CTGCAG	1 cut(s)	343
BspMI	ACCTGC	3 cut(s)	910, 1133, 2722
BspOI	GCTAGC	1 cut(s)	1829
BspPI	GGATC	4 cut(s)	113, 500, 1542, 1617
BspQI	GCTCTTC	1 cut(s)	1377
BspT104I	TTCGAA	1 cut(s)	2527
BspTI	CTTAAG	1 cut(s)	314
BspTNI	GGTCTC	1 cut(s)	1115
BsrDI	GCAATG	3 cut(s)	582, 1107, 2460
BsrFI	RCCGGY	3 cut(s)	23, 1580, 1905
BsrI	ACTGG	7 cut(s)	568, 1028, 1390, 1433, 1796, 2127, 2831
BssAI	RCCGGY	3 cut(s)	23, 1580, 1905
BssECI	CCNNGG	8 cut(s)	43, 199, 385, 1086, 1637, 1910, 2049, 2146
BssNI	GRCGYC	3 cut(s)	15, 1633, 2221
BssT1I	CCWWGG	4 cut(s)	43, 199, 1910, 2049
Bst2UI	CCWGG	3 cut(s)	750, 1087, 1532
Bst4CI	ACNGT	7 cut(s)	406, 525, 781, 1225, 2096, 2585, 2918
Bst6I	CTCTTC	2 cut(s)	1377, 1674
BstACI	GRCGYC	3 cut(s)	15, 1633, 2221
BstAFI	CTTAAG	1 cut(s)	314
BstAPI	GCANNNNNTGC	2 cut(s)	820, 1214
BstBAI	YACGTR	2 cut(s)	1339, 2943
BstBI	TTCGAA	1 cut(s)	2527
BstDSI	CCRYGG	2 cut(s)	385, 2146
BstENI	CCTNNNNNAGG	1 cut(s)	2420
BstF5I	GGATG	6 cut(s)	477, 846, 1887, 1942, 2707, 2929
BstFNI	CGCG	3 cut(s)	93, 2148, 2226
BstH2I	RGCGCY	1 cut(s)	756
BstHHI	GCGC	4 cut(s)	95, 640, 755, 1814
BstMAI	GTCTC	3 cut(s)	24, 1087, 1115
BstMCI	CGRYCG	3 cut(s)	91, 1847, 2805
BstNI	CCWGG	3 cut(s)	750, 1087, 1532
BstNSI	RCATGY	2 cut(s)	552, 2537
BstSCI	CCNGG	3 cut(s)	748, 1085, 1530
BstSFI	CTRYAG	2 cut(s)	339, 1725
BstSLI	GKGCMC	1 cut(s)	1869
BstUI	CGCG	3 cut(s)	93, 2148, 2226
BstV2I	GAAGAC	5 cut(s)	120, 126, 397, 1049, 1832
BstX2I	RGATCY	2 cut(s)	1534, 1609
BstXI	CCANNNNNNTGG	1 cut(s)	1878
BstYI	RGATCY	2 cut(s)	1534, 1609
Bsu15I	ATCGAT	2 cut(s)	117, 1153
Bsu36I	CCTNAGG	1 cut(s)	1360
BsuI	GTATCC	5 cut(s)	394, 1893, 2217, 2467, 2906
BsuRI	GGCC	6 cut(s)	48, 57, 252, 1318, 1909, 2326
BsuTUI	ATCGAT	2 cut(s)	117, 1153
BtgI	CCRYGG	2 cut(s)	385, 2146
BtsCI	GGATG	6 cut(s)	477, 846, 1887, 1942, 2707, 2929
BtsIMutI	CAGTG	6 cut(s)	521, 772, 1163, 2092, 2590, 2690
BtuMI	TCGCGA	1 cut(s)	2226
BveI	ACCTGC	3 cut(s)	910, 1133, 2722
CaiI	CAGNNNCTG	1 cut(s)	755
CfoI	GCGC	4 cut(s)	95, 640, 755, 1814
Cfr10I	RCCGGY	3 cut(s)	23, 1580, 1905
Cfr13I	GGNCC	6 cut(s)	55, 222, 251, 614, 644, 2325
Cfr42I	CCGCGG	1 cut(s)	2149
ClaI	ATCGAT	2 cut(s)	117, 1153
CseI	GACGC	2 cut(s)	1622, 2210
Csp6I	GTAC	6 cut(s)	420, 552, 1231, 1934, 2086, 2352
CspCI	CAANNNNNGTGG	2 cut(s)	2175, 2210
CviAII	CATG	9 cut(s)	236, 444, 549, 815, 1972, 2030, 2534, 2857, 2866
CviQI	GTAC	6 cut(s)	420, 552, 1231, 1934, 2086, 2352
DriI	GACNNNNNGTC	1 cut(s)	2166
Eam1104I	CTCTTC	2 cut(s)	1377, 1674
Eam1105I	GACNNNNNGTC	1 cut(s)	2166
EarI	CTCTTC	2 cut(s)	1377, 1674
EciI	GGCGGA	2 cut(s)	42, 2714
Ecl136II	GAGCTC	2 cut(s)	186, 704
Eco130I	CCWWGG	4 cut(s)	43, 199, 1910, 2049
Eco24I	GRGCYC	4 cut(s)	188, 706, 2493, 2593
Eco31I	GGTCTC	1 cut(s)	1115
Eco32I	GATATC	1 cut(s)	1426
Eco47I	GGWCC	3 cut(s)	222, 614, 644
Eco53kI	GAGCTC	2 cut(s)	186, 704
Eco57I	CTGAAG	2 cut(s)	2157, 2780
Eco81I	CCTNAGG	1 cut(s)	1360
Eco88I	CYCGRG	1 cut(s)	2788
EcoICRI	GAGCTC	2 cut(s)	186, 704
EcoNI	CCTNNNNNAGG	1 cut(s)	2420
EcoO109I	RGGNCCY	1 cut(s)	222
EcoRI	GAATTC	1 cut(s)	2767
EcoRII	CCWGG	3 cut(s)	748, 1085, 1530
EcoRV	GATATC	1 cut(s)	1426
EcoT14I	CCWWGG	4 cut(s)	43, 199, 1910, 2049
EcoT22I	ATGCAT	2 cut(s)	816, 2535
EcoT38I	GRGCYC	4 cut(s)	188, 706, 2493, 2593
ErhI	CCWWGG	4 cut(s)	43, 199, 1910, 2049
Esp3I	CGTCTC	1 cut(s)	24
FaeI	CATG	9 cut(s)	239, 447, 552, 818, 1975, 2033, 2537, 2860, 2869
FalI	AAGNNNNNCTT	2 cut(s)	2414, 2446
FaqI	GGGAC	4 cut(s)	425, 1103, 1802, 2698
FatI	CATG	9 cut(s)	235, 443, 548, 814, 1971, 2029, 2533, 2856, 2865
FauI	CCCGC	1 cut(s)	157
FokI	GGATG	6 cut(s)	464, 833, 1874, 1929, 2714, 2936
FriOI	GRGCYC	4 cut(s)	188, 706, 2493, 2593
FspBI	CTAG	8 cut(s)	351, 357, 1050, 1616, 1826, 1850, 2015, 2892
GlaI	GCGC	4 cut(s)	94, 639, 754, 1813
GsaI	CCCAGC	5 cut(s)	759, 1328, 1629, 1788, 2846
GsuI	CTGGAG	2 cut(s)	720, 2814
HaeII	RGCGCY	1 cut(s)	756
HaeIII	GGCC	6 cut(s)	48, 57, 252, 1318, 1909, 2326
HapII	CCGG	4 cut(s)	24, 49, 1581, 1906
HgaI	GACGC	2 cut(s)	1622, 2210
HhaI	GCGC	4 cut(s)	95, 640, 755, 1814
Hin1I	GRCGYC	3 cut(s)	15, 1633, 2221
Hin1II	CATG	9 cut(s)	239, 447, 552, 818, 1975, 2033, 2537, 2860, 2869
Hin6I	GCGC	4 cut(s)	93, 638, 753, 1812
HinP1I	GCGC	4 cut(s)	93, 638, 753, 1812
HincII	GTYRAC	2 cut(s)	2667, 2836
HindII	GTYRAC	2 cut(s)	2667, 2836
HindIII	AAGCTT	1 cut(s)	2428
HinfI	GANTC	5 cut(s)	263, 290, 518, 987, 2603
HpaII	CCGG	4 cut(s)	24, 49, 1581, 1906
HphI	GGTGA	5 cut(s)	373, 377, 1808, 2033, 2209
Hpy166II	GTNNAC	6 cut(s)	614, 1469, 1588, 1867, 2667, 2836
Hpy8I	GTNNAC	6 cut(s)	614, 1469, 1588, 1867, 2667, 2836
Hpy99I	CGWCG	5 cut(s)	20, 38, 86, 89, 2226
HpyCH4III	ACNGT	7 cut(s)	406, 525, 781, 1225, 2096, 2585, 2918
HpyCH4IV	ACGT	4 cut(s)	15, 1010, 1338, 2942
HpySE526I	ACGT	4 cut(s)	15, 1010, 1338, 2942
Hsp92I	GRCGYC	3 cut(s)	15, 1633, 2221
Hsp92II	CATG	9 cut(s)	239, 447, 552, 818, 1975, 2033, 2537, 2860, 2869
HspAI	GCGC	4 cut(s)	93, 638, 753, 1812
KroI	GCCGGC	2 cut(s)	23, 1905
KroNI	GCCGGC	2 cut(s)	25, 1907
KspI	CCGCGG	1 cut(s)	2149
LguI	GCTCTTC	1 cut(s)	1377
LweI	GCATC	8 cut(s)	948, 1609, 1779, 2035, 2062, 2190, 2382, 2470
MaeI	CTAG	8 cut(s)	351, 357, 1050, 1616, 1826, 1850, 2015, 2892
MaeII	ACGT	4 cut(s)	15, 1010, 1338, 2942
MaeIII	GTNAC	6 cut(s)	532, 841, 988, 1126, 2551, 2929
MflI	RGATCY	2 cut(s)	1534, 1609
MhlI	GDGCHC	7 cut(s)	188, 706, 1393, 1869, 2493, 2593, 2811
MlyI	GAGTC	1 cut(s)	996
MmeI	TCCRAC	1 cut(s)	634
Mph1103I	ATGCAT	2 cut(s)	816, 2535
MroNI	GCCGGC	2 cut(s)	23, 1905
MseI	TTAA	7 cut(s)	315, 783, 791, 801, 1005, 1440, 2234
MslI	CAYNNNNRTG	5 cut(s)	179, 1337, 1727, 2034, 2195
MspA1I	CMGCKG	3 cut(s)	1180, 1629, 2148
MspCI	CTTAAG	1 cut(s)	314
MspI	CCGG	4 cut(s)	24, 49, 1581, 1906
MspR9I	CCNGG	3 cut(s)	750, 1087, 1532
Mva1269I	GAATGC	4 cut(s)	1328, 1496, 1718, 2535
MvaI	CCWGG	3 cut(s)	750, 1087, 1532
MvnI	CGCG	3 cut(s)	93, 2148, 2226
NaeI	GCCGGC	2 cut(s)	25, 1907
NgoMIV	GCCGGC	2 cut(s)	23, 1905
NheI	GCTAGC	1 cut(s)	1825
NlaIII	CATG	9 cut(s)	239, 447, 552, 818, 1975, 2033, 2537, 2860, 2869
NlaIV	GGNNCC	6 cut(s)	56, 223, 253, 1529, 1661, 2592
NmuCI	GTSAC	3 cut(s)	841, 988, 1126
NruI	TCGCGA	1 cut(s)	2226
NsiI	ATGCAT	2 cut(s)	816, 2535
NspI	RCATGY	2 cut(s)	552, 2537
NspV	TTCGAA	1 cut(s)	2527
OliI	CACNNNNGTG	1 cut(s)	1727
PaeI	GCATGC	1 cut(s)	2537
PaeR7I	CTCGAG	1 cut(s)	2788
PaqCI	CACCTGC	2 cut(s)	910, 2722
PciI	ACATGT	1 cut(s)	548
PciSI	GCTCTTC	1 cut(s)	1377
PcsI	WCGNNNNNNNCGW	1 cut(s)	84
PctI	GAATGC	4 cut(s)	1328, 1496, 1718, 2535
PdiI	GCCGGC	2 cut(s)	25, 1907
PfeI	GAWTC	4 cut(s)	263, 290, 518, 2603
PflMI	CCANNNNNTGG	2 cut(s)	653, 755
Ple19I	CGATCG	2 cut(s)	91, 1847
PleI	GAGTC	1 cut(s)	995
PpsI	GAGTC	1 cut(s)	995
Ppu21I	YACGTR	2 cut(s)	1339, 2943
PpuMI	RGGWCCY	1 cut(s)	222
PscI	ACATGT	1 cut(s)	548
PsiI	TTATAA	2 cut(s)	1520, 2619
Psp124BI	GAGCTC	2 cut(s)	188, 706
Psp5II	RGGWCCY	1 cut(s)	222
Psp6I	CCWGG	3 cut(s)	748, 1085, 1530
PspFI	CCCAGC	5 cut(s)	755, 1324, 1625, 1784, 2842
PspGI	CCWGG	3 cut(s)	748, 1085, 1530
PspN4I	GGNNCC	6 cut(s)	56, 223, 253, 1529, 1661, 2592
PspPI	GGNCC	6 cut(s)	55, 222, 251, 614, 644, 2325
PspPPI	RGGWCCY	1 cut(s)	222
PstI	CTGCAG	1 cut(s)	343
PstNI	CAGNNNCTG	1 cut(s)	755
PsuI	RGATCY	2 cut(s)	1534, 1609
PvuI	CGATCG	2 cut(s)	91, 1847
PvuII	CAGCTG	1 cut(s)	1629
RruI	TCGCGA	1 cut(s)	2226
RsaI	GTAC	6 cut(s)	421, 553, 1232, 1935, 2087, 2353
RsaNI	GTAC	6 cut(s)	420, 552, 1231, 1934, 2086, 2352
RseI	CAYNNNNRTG	5 cut(s)	179, 1337, 1727, 2034, 2195
SacI	GAGCTC	2 cut(s)	188, 706
SacII	CCGCGG	1 cut(s)	2149
SapI	GCTCTTC	1 cut(s)	1377
SaqAI	TTAA	7 cut(s)	315, 783, 791, 801, 1005, 1440, 2234
Sau96I	GGNCC	6 cut(s)	55, 222, 251, 614, 644, 2325
SchI	GAGTC	1 cut(s)	996
ScrFI	CCNGG	3 cut(s)	750, 1087, 1532
SduI	GDGCHC	7 cut(s)	188, 706, 1393, 1869, 2493, 2593, 2811
SfaNI	GCATC	8 cut(s)	948, 1609, 1779, 2035, 2062, 2190, 2382, 2470
SfcI	CTRYAG	2 cut(s)	339, 1725
SfiI	GGCCNNNNNGGCC	1 cut(s)	54
Sfr274I	CTCGAG	1 cut(s)	2788
Sfr303I	CCGCGG	1 cut(s)	2149
SfuI	TTCGAA	1 cut(s)	2527
SgrBI	CCGCGG	1 cut(s)	2149
SinI	GGWCC	3 cut(s)	222, 614, 644
SlaI	CTCGAG	1 cut(s)	2788
SmiMI	CAYNNNNRTG	5 cut(s)	179, 1337, 1727, 2034, 2195
SmlI	CTYRAG	7 cut(s)	101, 167, 314, 2180, 2492, 2595, 2788
SmoI	CTYRAG	7 cut(s)	101, 167, 314, 2180, 2492, 2595, 2788
SphI	GCATGC	1 cut(s)	2537
SspI	AATATT	2 cut(s)	304, 373
SspMI	CTAG	8 cut(s)	351, 357, 1050, 1616, 1826, 1850, 2015, 2892
SstI	GAGCTC	2 cut(s)	188, 706
StyD4I	CCNGG	3 cut(s)	748, 1085, 1530
StyI	CCWWGG	4 cut(s)	43, 199, 1910, 2049
TaaI	ACNGT	7 cut(s)	406, 525, 781, 1225, 2096, 2585, 2918
TaiI	ACGT	4 cut(s)	18, 1013, 1341, 2945
TaqI	TCGA	6 cut(s)	18, 117, 1153, 1843, 2527, 2789
TaqII	GACCGA	2 cut(s)	409, 1097
TatI	WGTACW	2 cut(s)	551, 2085
TauI	GCSGC	2 cut(s)	60, 2151
TfiI	GAWTC	4 cut(s)	263, 290, 518, 2603
Tru1I	TTAA	7 cut(s)	315, 783, 791, 801, 1005, 1440, 2234
Tru9I	TTAA	7 cut(s)	315, 783, 791, 801, 1005, 1440, 2234
TscAI	CASTG	6 cut(s)	528, 772, 1170, 2099, 2590, 2697
TseFI	GTSAC	3 cut(s)	841, 988, 1126
Tsp45I	GTSAC	3 cut(s)	841, 988, 1126
TspDTI	ATGAA	7 cut(s)	617, 1321, 1988, 2046, 2573, 2616, 2675
TspGWI	ACGGA	1 cut(s)	470
TspRI	CASTG	6 cut(s)	528, 772, 1170, 2099, 2590, 2697
Van91I	CCANNNNNTGG	2 cut(s)	653, 755
Vha464I	CTTAAG	1 cut(s)	314
VneI	GTGCAC	1 cut(s)	1865
VpaK11BI	GGWCC	3 cut(s)	222, 614, 644
XagI	CCTNNNNNAGG	1 cut(s)	2420
XapI	RAATTY	4 cut(s)	1646, 2241, 2445, 2767
XbaI	TCTAGA	1 cut(s)	2014
XceI	RCATGY	2 cut(s)	552, 2537
XhoI	CTCGAG	1 cut(s)	2788
XspI	CTAG	8 cut(s)	351, 357, 1050, 1616, 1826, 1850, 2015, 2892
ZraI	GACGTC	1 cut(s)	16
Zsp2I	ATGCAT	2 cut(s)	816, 2535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.