Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0003023

Physical Location & Seq

Genomic Coordinates Forward (+)

12119637 .. 12128568

Gene Structure 8932 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032213

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2784 bp

ATGGGCAGGAACATAAAAGTGAGCTCTGAGGAGAACCTTGGCTCTAACATAAAAGAAGGGTCCTCTGCTAGTCATGTGCAAGCCAACGGCCCCCAGAAAGAATCCAAATTGGAACTCTTTGGCTTTGACTCTCTTGTCAATATTCTTGGTCTTAAGAGTATGACAGATGAGCAAGCTGCAGCACCATCTAGTCCTAGAGATGGTGAAAATATTGCTATCACCCATGGAAGACCGAAGGATACTGCCGTCAAATCGGGTACATTGATGGGTGTATTTGTCCCATGCCTGCAAAACATATTGGGAATTATCTACTACATCCGTTTTTCTTGGATCGTTGGTATGGCTGGCATAGCTGATTCACTGTTGGTGGTTACATTATGTGGCACATGTACTTTCCTGACTGGAATATCATTGAGTGCCATTGCAACCAATGGTGCTATGAAGGGAGGTGGACCATACTATCTAATTGGTCGTGCGCTTGGTCCAGAAGTTGGAGTTAGCATTGGGTTATGTTTCTTTCTTGGAAATGCTGTTGCTGGAGCTCTTTATGTCTTGGGAGCTGTGGAAACCTTCTTGAAAGCTCTGCCAGGCGCTGGGTTTTTCAGTGAAACAACAACAGTTAATGACTTGAAGACTTTTAATATGAGTGTGCATGATTTGCAAATCTATGGGATAATTGTGACCATCCTATTGTGCTTTATTGTGTTTGGGGGAGTGAAAATAATCAATAAGGTTGCACCTGCTTTCCTCATACCCGTTCTATTGTCGATGCTCTGCATCTATATCGGGATTGCTCTGGCAAGGAAGGATTACCCTACAAAGGGAGTCACGGGCTTTAGTTTTCATACGTTCAAAGATAACTGGAAATCAGATTATCAGAAGACTACTAGCGGTGGAGTTCCTGACCCTGACGGAGCTGTCTCCTGGGACTTCAATGCAATGGTCGGTCTCTTTTTCCCTGCCGTGACAGGAATTATGGCAGGTTCAAATCGATCAGCCTCACTGAAAGACACTCAGCGGTCAATTCCTATTGGAACATTGGCTGCAACTCTTGCAACTACTGTTATGTACCTGATCTCTGTGTTAGTATTTGGAGGACTTGCCACCAGACATTTGCTTTTGACTAACAGGCTCCTTTCGGCTACTATATCTTGGCCTTTCCCAGCATTCATTTACGTGGGAATAATTCTTTCAACCTTAGGTGCTGCTCTTCAAAGCCTGACTGGTGCTCCCCGTCTGCTTGCTGCAATAGCCAATGATGATATCTTACCAGTTCTTAACTACTTCAAGGTTGCAGATGGGAGTGAACCTCACTTTGCTACCTTATTTACCGCATTCCTTTGTATTGCGTGTGTTATAATTGGGAACCTGGATCTTATCACACCAACTATAACTATGTTTTTCCTTCTGTGTTATGCCGGTGTGAACTTATCTTGCTTCCTTCTGGATCTCCTAGATGCTCCCAGCTGGCGTCCTCGGTGGAAATTCCACCATTGGAGCCTCTCTCTTCTTGGAGCTTCACTATGTATAGTGATTATGTTCTTGATCTCTTGGGCATTCACTATAGTGTCGCTAGCCCTTGCAAGCCTTATATATTATTATGTGAGCATCAAAGGAAAGGCTGGGGACTGGGGTGATGGTTTCAAGAGCGCATATTTTCAGCTAGCTCTCCGAAGTCTTCGATCGCTAGGAGCAAACCAAGTGCACCCAAAGAATTGGTATCCCATCCCCCTGATATTCTGCCGGCCTTGGGGGAAGCTGCCAGATAATGTACCCTGCCATCCCAAACTTGCCGACTTCGCCAACTGCATGAAGAAGAAGGGCAGGGGAATGTCTATCTTTGTCTCTATTCTAGACGGTGATTACCATGAATGTGTTGAAGATGCCAAGGCTGCCTGCAAGCAGCTTGATGCTTACATTGAGTACAAGCACTGTGAAGGTGTAGCTGAGATTGTTGTCGCCCCCAGTATGTCTGAAGGCTTCCGCGGCATTGTCCAGACAATGGGTCTTGGCAATCTCAAGCCAAACATAGTGGTGATGCGGTATCCTGAAATATGGCGTCGCGAAAACTTAACAGAAATTCCAAACACCTTTGTCGAAATAATAAATGATTGCATAGTTGCAAACAAGGCAGTAGTTATTGTCAAAGGTCTTGACGAATGGCCCAATGAGTTTCAGAGGCAGTATGGTACTATTGATCTCTATTGGATAGTAAGAGATGGAGGTCTGATGCTTCTTCTCTCTCAGCTCCTCCTCACTAAGGAAAGCTTTGAAAGTTGTAAAATTCAAGTTTTCTGCATTGCGGAGGAGGATACTGATGCAGAGGGGCTCAAGGCTGATGTGAAGAAGTTTCTGTATGATCTTCGAATGCATGCAGAAGTCATTGTTGTAACAATGAAGTCGTGGGATATGCAAGCAGACGGTGGGGCTCCTCAAGATGAATCTGTGGAGGCTTATAATGCTGCTCAAAAGCGGATAGCTGATTACTTGGCTGAGATGAAGTCAACAGCTATGAAACAAGGGACACCACTGATGGCGGATGGGAAACAGGTCGTTGTGAATGAGCAGCAGGTGGAGAAGTTTCTTTACACTACTCTGAAGTTGAATTCAACAATACTCAGATACTCGAGAATGGCTGCGGTCGTGCTCGTTAGCTTACCTCCTCCTCCAGTCAACCACCCAGCATACTTTTACATGGAATACATGGATTTGTTGGTGGAGAATGTGCCTAGACTTCTGATTGTGAGAGGATACCGTAGGGATGTTGTAACTCTATTCACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006820 GO:0006821 GO:0008150 GO:0008324 GO:0008509 GO:0008511 GO:0009674 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015103 GO:0015108 GO:0015291 GO:0015293 GO:0015294 GO:0015296 GO:0015318 GO:0015370 GO:0015373 GO:0015377 GO:0015378 GO:0015379 GO:0015672 GO:0015698 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0034220 GO:0035725 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055064 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055081 GO:0055083 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097708 GO:0098655 GO:0098656 GO:0098660 GO:0098661 GO:0098662 GO:0098771 GO:0098791 GO:1902476

KEGG Pathways

Metabolic & Signaling

ko04966 map04966

Pfam Domains

Protein Families

AA_permease SLC12

Protein Analysis

928

Amino Acids

101.59

Weight (kDa)

6.91

Isoelectric Point (pI)

34.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease	PF00324	90 - 553	2.2e-55	Amino acid permease
AA_permease_2	PF13520	112 - 534	7.5e-16	Amino acid permease
SLC12	PF03522	595 - 716	5e-13	Solute carrier family 12
SLC12	PF03522	725 - 926	4.1e-26	Solute carrier family 12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30450 AT1G30450 AT1G30450 AT1G30450
fragaria_vesca	FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910
malus_domestica	MD05G1275000.v1.1 MD10G1254500.v1.1
prunus_persica	Prupe.4G088900_v2.0.a1 Prupe.4G088900_v2.0.a1
pyrus_communis	pycom05g25640 pycom10g21010
rosa_chinensis	RchiOBHm_Chr5g0015611 RchiOBHm_Chr5g0015801 RchiOBHm_Chr5g0015811
rosa_laevigata	RLG00000032213 RLG00000032223
rosa_multiflora	Rmu_co8164906.1_g000001 Rmu_sc0003989.1_g000002 Rmu_sc0005803.1_g000020 Rmu_sc0007629.1_g000001 Rmu_sc0011313.1_g000001 Rmu_sc0011313.1_g000002 Rmu_sc0016973.1_g000001
rosa_roxburghii	Rroxscaffold_1G00060860
rosa_rugosa	Rorug05G0028900
rosa_samantha	Rh5AG122200 Rh5BG120800 Rh5CG131200 Rh5CG132900 Rh5CG133000 Rh5DG119800 Rh5DG120900
rosa_wichuraiana	Rw5G010540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1358, 2457
AarI	CACCTGC	2 cut(s)	748, 2560
AasI	GACNNNNNNGTC	2 cut(s)	134, 917
Acc36I	ACCTGC	3 cut(s)	748, 971, 2560
AccB7I	CCANNNNNTGG	3 cut(s)	491, 593, 2002
AccII	CGCG	2 cut(s)	1986, 2064
AclWI	GGATC	3 cut(s)	338, 1380, 1455
AcsI	RAATTY	4 cut(s)	1484, 2079, 2283, 2605
AcuI	CTGAAG	2 cut(s)	1995, 2618
AcyI	GRCGYC	2 cut(s)	1471, 2059
AfaI	GTAC	6 cut(s)	259, 391, 1070, 1773, 1925, 2191
AfiI	CCNNNNNNNGG	8 cut(s)	200, 491, 593, 820, 821, 1750, 2002, 2260
AflII	CTTAAG	1 cut(s)	152
AflIII	ACRYGT	1 cut(s)	386
AhdI	GACNNNNNGTC	1 cut(s)	2004
AjnI	CCWGG	3 cut(s)	586, 923, 1368
AleI	CACNNNNGTG	1 cut(s)	1565
Alw21I	GWGCWC	5 cut(s)	26, 544, 1231, 1707, 2649
Alw26I	GTCTC	3 cut(s)	925, 953, 1849
Alw44I	GTGCAC	1 cut(s)	1703
AlwI	GGATC	3 cut(s)	338, 1380, 1455
AlwNI	CAGNNNCTG	1 cut(s)	593
Ama87I	CYCGRG	1 cut(s)	2626
AoxI	GGCC	4 cut(s)	88, 1154, 1745, 2162
ApaLI	GTGCAC	1 cut(s)	1703
ApoI	RAATTY	4 cut(s)	1484, 2079, 2283, 2605
AspLEI	GCGC	3 cut(s)	478, 593, 1652
AspS9I	GGNCC	5 cut(s)	60, 89, 452, 482, 2163
AsuHPI	GGTGA	5 cut(s)	211, 215, 1646, 1871, 2047
AsuII	TTCGAA	1 cut(s)	2365
AsuNHI	GCTAGC	2 cut(s)	1573, 1663
AvaI	CYCGRG	1 cut(s)	2626
AvaII	GGWCC	3 cut(s)	60, 452, 482
AxyI	CCTNAGG	1 cut(s)	1198
BaeGI	GKGCMC	1 cut(s)	1707
BaeI	ACNNNNGTAYC	2 cut(s)	231, 264
BanII	GRGCYC	4 cut(s)	26, 544, 2331, 2431
BarI	GAAGNNNNNNTAC	2 cut(s)	2570, 2602
BbsI	GAAGAC	4 cut(s)	235, 638, 887, 1670
Bbv12I	GWGCWC	5 cut(s)	26, 544, 1231, 1707, 2649
BceAI	ACGGC	3 cut(s)	103, 230, 947
BcgI	CGANNNNNNTGC	2 cut(s)	766, 800
BciT130I	CCWGG	3 cut(s)	588, 925, 1370
BciVI	GTATCC	5 cut(s)	232, 1731, 2055, 2305, 2744
BcoDI	GTCTC	3 cut(s)	925, 953, 1849
BfmI	CTRYAG	2 cut(s)	177, 1563
BfoI	RGCGCY	1 cut(s)	594
BfrI	CTTAAG	1 cut(s)	152
BfuAI	ACCTGC	3 cut(s)	748, 971, 2560
BfuI	GTATCC	5 cut(s)	232, 1731, 2055, 2305, 2744
Bme1390I	CCNGG	3 cut(s)	588, 925, 1370
Bme18I	GGWCC	3 cut(s)	60, 452, 482
BmeRI	GACNNNNNGTC	1 cut(s)	2004
BmeT110I	CYCGRG	1 cut(s)	2626
BmgT120I	GGNCC	5 cut(s)	60, 89, 452, 482, 2163
BmiI	GGNNCC	6 cut(s)	61, 91, 1133, 1367, 1499, 2430
BmrFI	CCNGG	3 cut(s)	588, 925, 1370
BmrI	ACTGGG	2 cut(s)	1639, 1959
BmsI	GCATC	9 cut(s)	759, 786, 1447, 1617, 1873, 1900, 2028, 2220, 2308
BmtI	GCTAGC	2 cut(s)	1577, 1667
BmuI	ACTGGG	2 cut(s)	1639, 1959
BpiI	GAAGAC	4 cut(s)	235, 638, 887, 1670
BplI	GAGNNNNNCTC	4 cut(s)	1294, 1326, 1489, 1521
BpmI	CTGGAG	2 cut(s)	558, 2652
Bpu14I	TTCGAA	1 cut(s)	2365
BpuEI	CTTGAG	3 cut(s)	2003, 2315, 2418
Bsa29I	ATCGAT	1 cut(s)	991
BsaAI	YACGTR	2 cut(s)	1177, 2781
BsaHI	GRCGYC	2 cut(s)	1471, 2059
BsaI	GGTCTC	1 cut(s)	953
BsaJI	CCNNGG	7 cut(s)	37, 223, 924, 1475, 1748, 1887, 1984
BsaXI	ACNNNNNCTCC	2 cut(s)	1213, 1243
Bsc4I	CCNNNNNNNGG	8 cut(s)	200, 491, 593, 820, 821, 1750, 2002, 2260
Bse118I	RCCGGY	2 cut(s)	1418, 1743
Bse1I	ACTGG	7 cut(s)	406, 866, 1228, 1271, 1634, 1965, 2669
Bse21I	CCTNAGG	1 cut(s)	1198
Bse3DI	GCAATG	3 cut(s)	420, 945, 2298
BseBI	CCWGG	3 cut(s)	588, 925, 1370
BseCI	ATCGAT	1 cut(s)	991
BseDI	CCNNGG	7 cut(s)	37, 223, 924, 1475, 1748, 1887, 1984
BseGI	GGATG	6 cut(s)	315, 684, 1725, 1780, 2545, 2767
BseLI	CCNNNNNNNGG	8 cut(s)	200, 491, 593, 820, 821, 1750, 2002, 2260
BseMI	GCAATG	3 cut(s)	420, 945, 2298
BseMII	CTCAG	6 cut(s)	18, 1028, 1938, 2258, 2484, 2632
BseNI	ACTGG	7 cut(s)	406, 866, 1228, 1271, 1634, 1965, 2669
BseRI	GAGGAG	7 cut(s)	44, 2240, 2243, 2321, 2421, 2652, 2655
BseSI	GKGCMC	1 cut(s)	1707
BseYI	CCCAGC	5 cut(s)	593, 1162, 1463, 1622, 2680
Bsh1236I	CGCG	2 cut(s)	1986, 2064
Bsh1285I	CGRYCG	2 cut(s)	1685, 2643
BshFI	GGCC	4 cut(s)	90, 1156, 1747, 2164
BshVI	ATCGAT	1 cut(s)	991
BsiEI	CGRYCG	2 cut(s)	1685, 2643
BsiHKAI	GWGCWC	5 cut(s)	26, 544, 1231, 1707, 2649
BsiHKCI	CYCGRG	1 cut(s)	2626
BsiSI	CCGG	2 cut(s)	1419, 1744
BslFI	GGGAC	4 cut(s)	263, 941, 1640, 2536
BslI	CCNNNNNNNGG	8 cut(s)	200, 491, 593, 820, 821, 1750, 2002, 2260
BsmAI	GTCTC	3 cut(s)	925, 953, 1849
BsmFI	GGGAC	4 cut(s)	263, 941, 1640, 2536
BsmI	GAATGC	4 cut(s)	1166, 1334, 1556, 2373
BsnI	GGCC	4 cut(s)	90, 1156, 1747, 2164
Bso31I	GGTCTC	1 cut(s)	953
BsoBI	CYCGRG	1 cut(s)	2626
Bsp119I	TTCGAA	1 cut(s)	2365
Bsp1286I	GDGCHC	7 cut(s)	26, 544, 1231, 1707, 2331, 2431, 2649
Bsp143I	GATC	9 cut(s)	330, 992, 1074, 1372, 1447, 1545, 1682, 2197, 2359
Bsp19I	CCATGG	1 cut(s)	223
Bsp68I	TCGCGA	1 cut(s)	2064
BspANI	GGCC	4 cut(s)	90, 1156, 1747, 2164
BspCNI	CTCAG	6 cut(s)	19, 1027, 1939, 2257, 2485, 2631
BspDI	ATCGAT	1 cut(s)	991
BspFNI	CGCG	2 cut(s)	1986, 2064
BspLI	GGNNCC	6 cut(s)	61, 91, 1133, 1367, 1499, 2430
BspMAI	CTGCAG	1 cut(s)	181
BspMI	ACCTGC	3 cut(s)	748, 971, 2560
BspOI	GCTAGC	2 cut(s)	1577, 1667
BspPI	GGATC	3 cut(s)	338, 1380, 1455
BspQI	GCTCTTC	1 cut(s)	1215
BspT104I	TTCGAA	1 cut(s)	2365
BspTI	CTTAAG	1 cut(s)	152
BspTNI	GGTCTC	1 cut(s)	953
BsrDI	GCAATG	3 cut(s)	420, 945, 2298
BsrFI	RCCGGY	2 cut(s)	1418, 1743
BsrI	ACTGG	7 cut(s)	406, 866, 1228, 1271, 1634, 1965, 2669
BssAI	RCCGGY	2 cut(s)	1418, 1743
BssECI	CCNNGG	7 cut(s)	37, 223, 924, 1475, 1748, 1887, 1984
BssMI	GATC	9 cut(s)	330, 992, 1074, 1372, 1447, 1545, 1682, 2197, 2359
BssNI	GRCGYC	2 cut(s)	1471, 2059
BssT1I	CCWWGG	4 cut(s)	37, 223, 1748, 1887
Bst2UI	CCWGG	3 cut(s)	588, 925, 1370
Bst4CI	ACNGT	7 cut(s)	363, 619, 1063, 1859, 1934, 2423, 2756
Bst6I	CTCTTC	2 cut(s)	1215, 1512
BstACI	GRCGYC	2 cut(s)	1471, 2059
BstAFI	CTTAAG	1 cut(s)	152
BstAPI	GCANNNNNTGC	2 cut(s)	658, 1052
BstBAI	YACGTR	2 cut(s)	1177, 2781
BstBI	TTCGAA	1 cut(s)	2365
BstDEI	CTNAG	8 cut(s)	27, 1014, 1198, 1947, 2244, 2259, 2493, 2618
BstDSI	CCRYGG	2 cut(s)	223, 1984
BstENI	CCTNNNNNAGG	1 cut(s)	2258
BstF5I	GGATG	6 cut(s)	315, 684, 1725, 1780, 2545, 2767
BstFNI	CGCG	2 cut(s)	1986, 2064
BstH2I	RGCGCY	1 cut(s)	594
BstHHI	GCGC	3 cut(s)	478, 593, 1652
BstKTI	GATC	9 cut(s)	333, 995, 1077, 1375, 1450, 1548, 1685, 2200, 2362
BstMAI	GTCTC	3 cut(s)	925, 953, 1849
BstMBI	GATC	9 cut(s)	330, 992, 1074, 1372, 1447, 1545, 1682, 2197, 2359
BstMCI	CGRYCG	2 cut(s)	1685, 2643
BstMWI	GCNNNNNNNGC	9 cut(s)	350, 658, 1052, 1250, 1799, 1892, 1986, 2129, 2459
BstNI	CCWGG	3 cut(s)	588, 925, 1370
BstNSI	RCATGY	2 cut(s)	390, 2375
BstSCI	CCNGG	3 cut(s)	586, 923, 1368
BstSFI	CTRYAG	2 cut(s)	177, 1563
BstSLI	GKGCMC	1 cut(s)	1707
BstUI	CGCG	2 cut(s)	1986, 2064
BstV2I	GAAGAC	4 cut(s)	235, 638, 887, 1670
BstX2I	RGATCY	2 cut(s)	1372, 1447
BstXI	CCANNNNNNTGG	1 cut(s)	1716
BstYI	RGATCY	2 cut(s)	1372, 1447
Bsu15I	ATCGAT	1 cut(s)	991
Bsu36I	CCTNAGG	1 cut(s)	1198
BsuI	GTATCC	5 cut(s)	232, 1731, 2055, 2305, 2744
BsuRI	GGCC	4 cut(s)	90, 1156, 1747, 2164
BsuTUI	ATCGAT	1 cut(s)	991
BtgI	CCRYGG	2 cut(s)	223, 1984
BtsCI	GGATG	6 cut(s)	315, 684, 1725, 1780, 2545, 2767
BtsIMutI	CAGTG	5 cut(s)	359, 610, 1001, 1930, 2528
BtuMI	TCGCGA	1 cut(s)	2064
BveI	ACCTGC	3 cut(s)	748, 971, 2560
CaiI	CAGNNNCTG	1 cut(s)	593
CfoI	GCGC	3 cut(s)	478, 593, 1652
Cfr10I	RCCGGY	2 cut(s)	1418, 1743
Cfr13I	GGNCC	5 cut(s)	60, 89, 452, 482, 2163
Cfr42I	CCGCGG	1 cut(s)	1987
ClaI	ATCGAT	1 cut(s)	991
CseI	GACGC	2 cut(s)	1460, 2048
Csp6I	GTAC	6 cut(s)	258, 390, 1069, 1772, 1924, 2190
CspCI	CAANNNNNGTGG	2 cut(s)	2013, 2048
CviQI	GTAC	6 cut(s)	258, 390, 1069, 1772, 1924, 2190
DdeI	CTNAG	8 cut(s)	27, 1014, 1198, 1947, 2244, 2259, 2493, 2618
DpnI	GATC	9 cut(s)	332, 994, 1076, 1374, 1449, 1547, 1684, 2199, 2361
DpnII	GATC	9 cut(s)	330, 992, 1074, 1372, 1447, 1545, 1682, 2197, 2359
DrdI	GACNNNNNNGTC	2 cut(s)	134, 917
DriI	GACNNNNNGTC	1 cut(s)	2004
DseDI	GACNNNNNNGTC	2 cut(s)	134, 917
Eam1104I	CTCTTC	2 cut(s)	1215, 1512
Eam1105I	GACNNNNNGTC	1 cut(s)	2004
EarI	CTCTTC	2 cut(s)	1215, 1512
EciI	GGCGGA	1 cut(s)	2552
Ecl136II	GAGCTC	2 cut(s)	24, 542
Eco130I	CCWWGG	4 cut(s)	37, 223, 1748, 1887
Eco24I	GRGCYC	4 cut(s)	26, 544, 2331, 2431
Eco31I	GGTCTC	1 cut(s)	953
Eco32I	GATATC	1 cut(s)	1264
Eco47I	GGWCC	3 cut(s)	60, 452, 482
Eco53kI	GAGCTC	2 cut(s)	24, 542
Eco57I	CTGAAG	2 cut(s)	1995, 2618
Eco81I	CCTNAGG	1 cut(s)	1198
Eco88I	CYCGRG	1 cut(s)	2626
EcoICRI	GAGCTC	2 cut(s)	24, 542
EcoNI	CCTNNNNNAGG	1 cut(s)	2258
EcoO109I	RGGNCCY	1 cut(s)	60
EcoRI	GAATTC	1 cut(s)	2605
EcoRII	CCWGG	3 cut(s)	586, 923, 1368
EcoRV	GATATC	1 cut(s)	1264
EcoT14I	CCWWGG	4 cut(s)	37, 223, 1748, 1887
EcoT22I	ATGCAT	1 cut(s)	2373
EcoT38I	GRGCYC	4 cut(s)	26, 544, 2331, 2431
ErhI	CCWWGG	4 cut(s)	37, 223, 1748, 1887
FalI	AAGNNNNNCTT	2 cut(s)	2252, 2284
FaqI	GGGAC	4 cut(s)	263, 941, 1640, 2536
FokI	GGATG	6 cut(s)	302, 671, 1712, 1767, 2552, 2774
FriOI	GRGCYC	4 cut(s)	26, 544, 2331, 2431
GlaI	GCGC	3 cut(s)	477, 592, 1651
GsaI	CCCAGC	5 cut(s)	597, 1166, 1467, 1626, 2684
GsuI	CTGGAG	2 cut(s)	558, 2652
HaeII	RGCGCY	1 cut(s)	594
HaeIII	GGCC	4 cut(s)	90, 1156, 1747, 2164
HapII	CCGG	2 cut(s)	1419, 1744
HgaI	GACGC	2 cut(s)	1460, 2048
HhaI	GCGC	3 cut(s)	478, 593, 1652
Hin1I	GRCGYC	2 cut(s)	1471, 2059
Hin6I	GCGC	3 cut(s)	476, 591, 1650
HinP1I	GCGC	3 cut(s)	476, 591, 1650
HincII	GTYRAC	2 cut(s)	2505, 2674
HindII	GTYRAC	2 cut(s)	2505, 2674
HindIII	AAGCTT	1 cut(s)	2266
HinfI	GANTC	5 cut(s)	101, 128, 356, 825, 2441
HpaII	CCGG	2 cut(s)	1419, 1744
HphI	GGTGA	5 cut(s)	211, 215, 1646, 1871, 2047
Hpy166II	GTNNAC	6 cut(s)	452, 1307, 1426, 1705, 2505, 2674
Hpy8I	GTNNAC	6 cut(s)	452, 1307, 1426, 1705, 2505, 2674
Hpy99I	CGWCG	1 cut(s)	2064
HpyCH4III	ACNGT	7 cut(s)	363, 619, 1063, 1859, 1934, 2423, 2756
HpyCH4IV	ACGT	3 cut(s)	848, 1176, 2780
HpyF10VI	GCNNNNNNNGC	9 cut(s)	350, 658, 1052, 1250, 1799, 1892, 1986, 2129, 2459
HpyF3I	CTNAG	8 cut(s)	27, 1014, 1198, 1947, 2244, 2259, 2493, 2618
HpySE526I	ACGT	3 cut(s)	848, 1176, 2780
Hsp92I	GRCGYC	2 cut(s)	1471, 2059
HspAI	GCGC	3 cut(s)	476, 591, 1650
KroI	GCCGGC	1 cut(s)	1743
KroNI	GCCGGC	1 cut(s)	1745
KspI	CCGCGG	1 cut(s)	1987
Kzo9I	GATC	9 cut(s)	330, 992, 1074, 1372, 1447, 1545, 1682, 2197, 2359
LguI	GCTCTTC	1 cut(s)	1215
LweI	GCATC	9 cut(s)	759, 786, 1447, 1617, 1873, 1900, 2028, 2220, 2308
MaeII	ACGT	3 cut(s)	848, 1176, 2780
MaeIII	GTNAC	6 cut(s)	370, 679, 826, 964, 2389, 2767
MalI	GATC	9 cut(s)	332, 994, 1076, 1374, 1449, 1547, 1684, 2199, 2361
MboI	GATC	9 cut(s)	330, 992, 1074, 1372, 1447, 1545, 1682, 2197, 2359
MflI	RGATCY	2 cut(s)	1372, 1447
MhlI	GDGCHC	7 cut(s)	26, 544, 1231, 1707, 2331, 2431, 2649
MlyI	GAGTC	2 cut(s)	122, 834
MmeI	TCCRAC	1 cut(s)	472
Mph1103I	ATGCAT	1 cut(s)	2373
MroNI	GCCGGC	1 cut(s)	1743
MseI	TTAA	5 cut(s)	153, 621, 639, 1278, 2072
MslI	CAYNNNNRTG	5 cut(s)	17, 1175, 1565, 1872, 2033
MspA1I	CMGCKG	3 cut(s)	1018, 1467, 1986
MspCI	CTTAAG	1 cut(s)	152
MspI	CCGG	2 cut(s)	1419, 1744
MspR9I	CCNGG	3 cut(s)	588, 925, 1370
Mva1269I	GAATGC	4 cut(s)	1166, 1334, 1556, 2373
MvaI	CCWGG	3 cut(s)	588, 925, 1370
MvnI	CGCG	2 cut(s)	1986, 2064
MwoI	GCNNNNNNNGC	9 cut(s)	350, 658, 1052, 1250, 1799, 1892, 1986, 2129, 2459
NaeI	GCCGGC	1 cut(s)	1745
NcoI	CCATGG	1 cut(s)	223
NdeII	GATC	9 cut(s)	330, 992, 1074, 1372, 1447, 1545, 1682, 2197, 2359
NgoMIV	GCCGGC	1 cut(s)	1743
NheI	GCTAGC	2 cut(s)	1573, 1663
NlaIV	GGNNCC	6 cut(s)	61, 91, 1133, 1367, 1499, 2430
NmuCI	GTSAC	3 cut(s)	679, 826, 964
NruI	TCGCGA	1 cut(s)	2064
NsiI	ATGCAT	1 cut(s)	2373
NspI	RCATGY	2 cut(s)	390, 2375
NspV	TTCGAA	1 cut(s)	2365
OliI	CACNNNNGTG	1 cut(s)	1565
PaeI	GCATGC	1 cut(s)	2375
PaeR7I	CTCGAG	1 cut(s)	2626
PaqCI	CACCTGC	2 cut(s)	748, 2560
PciI	ACATGT	1 cut(s)	386
PciSI	GCTCTTC	1 cut(s)	1215
PctI	GAATGC	4 cut(s)	1166, 1334, 1556, 2373
PdiI	GCCGGC	1 cut(s)	1745
PfeI	GAWTC	3 cut(s)	101, 356, 2441
PflMI	CCANNNNNTGG	3 cut(s)	491, 593, 2002
Ple19I	CGATCG	1 cut(s)	1685
PleI	GAGTC	2 cut(s)	122, 833
PpsI	GAGTC	2 cut(s)	122, 833
Ppu21I	YACGTR	2 cut(s)	1177, 2781
PpuMI	RGGWCCY	1 cut(s)	60
PscI	ACATGT	1 cut(s)	386
PsiI	TTATAA	2 cut(s)	1358, 2457
Psp124BI	GAGCTC	2 cut(s)	26, 544
Psp5II	RGGWCCY	1 cut(s)	60
Psp6I	CCWGG	3 cut(s)	586, 923, 1368
PspFI	CCCAGC	5 cut(s)	593, 1162, 1463, 1622, 2680
PspGI	CCWGG	3 cut(s)	586, 923, 1368
PspN4I	GGNNCC	6 cut(s)	61, 91, 1133, 1367, 1499, 2430
PspPI	GGNCC	5 cut(s)	60, 89, 452, 482, 2163
PspPPI	RGGWCCY	1 cut(s)	60
PstI	CTGCAG	1 cut(s)	181
PstNI	CAGNNNCTG	1 cut(s)	593
PsuI	RGATCY	2 cut(s)	1372, 1447
PvuI	CGATCG	1 cut(s)	1685
PvuII	CAGCTG	1 cut(s)	1467
RruI	TCGCGA	1 cut(s)	2064
RsaI	GTAC	6 cut(s)	259, 391, 1070, 1773, 1925, 2191
RsaNI	GTAC	6 cut(s)	258, 390, 1069, 1772, 1924, 2190
RseI	CAYNNNNRTG	5 cut(s)	17, 1175, 1565, 1872, 2033
SacI	GAGCTC	2 cut(s)	26, 544
SacII	CCGCGG	1 cut(s)	1987
SapI	GCTCTTC	1 cut(s)	1215
SaqAI	TTAA	5 cut(s)	153, 621, 639, 1278, 2072
Sau3AI	GATC	9 cut(s)	330, 992, 1074, 1372, 1447, 1545, 1682, 2197, 2359
Sau96I	GGNCC	5 cut(s)	60, 89, 452, 482, 2163
SchI	GAGTC	2 cut(s)	122, 834
ScrFI	CCNGG	3 cut(s)	588, 925, 1370
SduI	GDGCHC	7 cut(s)	26, 544, 1231, 1707, 2331, 2431, 2649
SfaNI	GCATC	9 cut(s)	759, 786, 1447, 1617, 1873, 1900, 2028, 2220, 2308
SfcI	CTRYAG	2 cut(s)	177, 1563
Sfr274I	CTCGAG	1 cut(s)	2626
Sfr303I	CCGCGG	1 cut(s)	1987
SfuI	TTCGAA	1 cut(s)	2365
SgrBI	CCGCGG	1 cut(s)	1987
SinI	GGWCC	3 cut(s)	60, 452, 482
SlaI	CTCGAG	1 cut(s)	2626
SmiMI	CAYNNNNRTG	5 cut(s)	17, 1175, 1565, 1872, 2033
SmlI	CTYRAG	5 cut(s)	152, 2018, 2330, 2433, 2626
SmoI	CTYRAG	5 cut(s)	152, 2018, 2330, 2433, 2626
SphI	GCATGC	1 cut(s)	2375
SspI	AATATT	2 cut(s)	142, 211
SstI	GAGCTC	2 cut(s)	26, 544
StyD4I	CCNGG	3 cut(s)	586, 923, 1368
StyI	CCWWGG	4 cut(s)	37, 223, 1748, 1887
TaaI	ACNGT	7 cut(s)	363, 619, 1063, 1859, 1934, 2423, 2756
TaiI	ACGT	3 cut(s)	851, 1179, 2783
TaqI	TCGA	6 cut(s)	767, 991, 1681, 2097, 2365, 2627
TaqII	GACCGA	2 cut(s)	247, 935
TatI	WGTACW	2 cut(s)	389, 1923
TauI	GCSGC	1 cut(s)	1989
TfiI	GAWTC	3 cut(s)	101, 356, 2441
Tru1I	TTAA	5 cut(s)	153, 621, 639, 1278, 2072
Tru9I	TTAA	5 cut(s)	153, 621, 639, 1278, 2072
TscAI	CASTG	5 cut(s)	366, 610, 1008, 1937, 2535
TseFI	GTSAC	3 cut(s)	679, 826, 964
Tsp45I	GTSAC	3 cut(s)	679, 826, 964
TspDTI	ATGAA	9 cut(s)	455, 833, 1159, 1826, 1884, 2411, 2454, 2513, 2528
TspGWI	ACGGA	2 cut(s)	308, 927
TspRI	CASTG	5 cut(s)	366, 610, 1008, 1937, 2535
Van91I	CCANNNNNTGG	3 cut(s)	491, 593, 2002
Vha464I	CTTAAG	1 cut(s)	152
VneI	GTGCAC	1 cut(s)	1703
VpaK11BI	GGWCC	3 cut(s)	60, 452, 482
XagI	CCTNNNNNAGG	1 cut(s)	2258
XapI	RAATTY	4 cut(s)	1484, 2079, 2283, 2605
XbaI	TCTAGA	1 cut(s)	1852
XceI	RCATGY	2 cut(s)	390, 2375
XhoI	CTCGAG	1 cut(s)	2626
Zsp2I	ATGCAT	1 cut(s)	2373

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.