Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003023

Physical Location & Seq

Genomic Coordinates Reverse (-)

34682213 .. 34692279

Gene Structure 10067 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1254500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2952 bp

ATGGACAATGTCGACGTCGAAGCCGGTGCGGAAGACGAGTTCCGTGGCCAGAGGGGTCGCAAGTACCGCCCCGTCGTCGACAACGATCGGGCCGTGCTCGAGATGTCGCCCATGGATCCTTCCTCCTCCTCTTCTTCGTCGTCTTCTCTCCCGGTTCACCAGGCTTCCCTCAAGAAAATAAAAGTGAGCACACAAGAAAACATGGGTTCTAACATAAATGAAGGGCCTTCTACTCATGTGCAAGCCAAGGGCCCCCAGAAAGAATCCAATTTGGAACTATTTGGTTTTGATTCTCTCGTCAACATTCTTGGTCTGAAGAGTATGACGGATGAGCAGACTGCAGCACCATCAAGTCCTAGAGATGGTGAAGATATTGCTATCACTCAGGGGCGACCAAAGCCAAGTGATCTCAAACTGGGAACATTAATGGGTGTATTTGTGCCGTGCCTTCAGAACATTTTGGGAATTATCTACTATATCCGATTTTCTTGGATTGTTGGTATGGCTGGCATAGCTGAGTCACTATTCTTGGTTTCCCTCTGTGGCTTGTGTACTTTCCTGACTGCAATATCATTGAGCGCAATTGCAACCAATGGTGCTATGAAGGGTGGTGGACCTTACTATCTAATTGGTCGTGCCCTTGGTCCAGAAGTTGGAGTTAGCATTGGATTATGTTTCTTTCTAGGAAATGCTGTTGCTGGATCTCTTTATGTCTTGGGAGCTGTTGAAACCTTCTTGAAAGCTGTGCCGGCAGCTGGGATTTTTAGAGAGACCACAAGAGTTAATGGAACATCAATTACCATACACAGTCCAAGTTCTCATGACCTGCAAATCTATGGGATAGTTGTGACTATTATTTTGTGCTTTATTGTGTTTGGTGGTGTGAAAATGATCAATCGGGTTGCACCTGCCTTCCTCATACCCGTTTTGTTCTCGCTGTTCTGCATATATATCGGGATTGCTTTGGCGAGGAAGAATCACCCTGTAGATGGGGTCACGGGCTTGAGTTCGCACTCTTTCAAAGAAAACTGGAGCTCAGATTATAAGAAGACCAATAACAATGGAATTCCTGATCCTGACGGAAAAGTATCCTGGAATTTCAATGCAATGGTCGGCCTCTTTTTCCCTGCTGTGACAGGAATTATGGCAGGTTCAAATCGGTCAGCCTCACTGAAAGATACTCAGCGTTCAATTCCTATTGGAACACTGGCTGCAACTCTTTCAACTACTGCAATGTATCTGGTCTCTGTGTTACTATTTGGAGCCCTTGCAACCAGGGAGAAGCTTTTGACTGACAGGCTACTCTCGGCTAGAATTGCTTGGCCTTTCCCAGTATTCATTTACATTGGAATAATTCTTTCAACCTTAGGTGCTGCTCTTCAAAGCCTGACTGGTGCCCCCCGTCTCCTTGCAGCAATAGCCAATGATGACATTTTACCCGTTCTTAACTACTTTCGGGTTTCAGAAGGGAATGAGCCTCACATTGCTACCTTGTTTACTGCGCTCCTCTGTATCGGGTGTGTCATTATTGGGAACCTGGATCTTATCACGCCAACTATAACTATGTTTTTCCTTCTGTGTTATGCGGGTGTGAACTTATCTTGCTTCCTTCTGGATCTTCTGGATGCTCCCAGTTGGCGTCCTCGGTGGAAATTTCACCACTGGAGCCTCTCTCTTCTTGGAGCCACACTTTGTATAGTGATCATGTTCTTGATCTCTTGGTCATTCACTATCGTGTCTCTAGCCCTGGCAAGCCTTATATATTATTATGTGAGCATCAAAGGCAAGGCTGGGGACTGGGGTGATGGTTTCAAGAGTGCATATTTCCAACTTGCTCTCCGCAGTCTTCGATCTCTAGGAGCAAACCAGGTACACCCAAAGAATTGGTATCCCATCCCCTTGATATTCTGCCGGCCATGGGGGAAGCTGCCAGAAAATGTGCCCTGCCATCCCAAACTTGCTGACTTTGCCAACTGTATGAAGAAGAAGGGCAGGGGAATGTCCATTTTTTTCTCTATTCTAGACGGTGATTACCGTGAATGTGCTGAAGATGCTAAGACTGCATGCAAACAGCTTGCGACCTACCTTGACTACAAGAATTGTGAAGGTGTAGCTGAGATTGTCGTGGCCCCCAGTATGTCTGAAGGCTTTCGGGGCATCGTCCAGACAATGGGTCTTGGAAACCTCAAGCCAAACATTGTGGTGATGCGGTATCCTGAGATATGGCGTCGTGAAAACTTAACGGAAATTCCAGCCACTTTCGTTGAAATAATTAACGATTGCATTATTGCGAACAAGGCCGTTGTCATCGTCAAGGGTCTTGATGAATGGCCTAATGAGTATCAGAGGCAGTATGGTACCATTGACTTGTACTGGATTGTGAGAGATGGAGGTCTCATGCTGCTTCTGTCCCAGCTCCTCCTCACGAAGGAAAGCTTTGAGAGTTGCAAAATCCAGGTTTTCTGCATCGCAGAGGAGGATACTGATGCGGAGGGGCTCAAGGCTGATGTGAAAAAGTTTCTATATGATCTTCGGATGCATGCTGAAGTGATTGTTGTAACAATGAAATCGTGGGATGTGCAAGCAGATGGTGGGTCTCCTCAAGATGAATCTGTGGAGGCGTTCACTGGTGCTCAGCGGCGGATAGCTGATTACATGGCTAACATGAAGGCAATAGCTGAGAAACAAGGGACCCCGCTGATGGCTGACGGGAAGCAAGTTTTTGTGGATGAACAGCAGGTGGAGAAGTTTCTTTACACTACCCTAAAGCTGAACTCGACAATACTCAGATACTCGAGAATGGCTGCGGTTGTGCTTGTGAGCTTACCTCCACCGCCGGCCAACCACCCTGCATATTTCTACATGGAGTACATGGATTTGCTGGTGGAGAACGTGCCGAGACTTCTGATGGTGAGAGGATACCGTAGAGACGTTGTAACTCTATTCACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006820 GO:0006821 GO:0008150 GO:0008324 GO:0008509 GO:0008511 GO:0009674 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015103 GO:0015108 GO:0015291 GO:0015293 GO:0015294 GO:0015296 GO:0015318 GO:0015370 GO:0015373 GO:0015377 GO:0015378 GO:0015379 GO:0015672 GO:0015698 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0034220 GO:0035725 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055064 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055081 GO:0055083 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097708 GO:0098655 GO:0098656 GO:0098660 GO:0098661 GO:0098662 GO:0098771 GO:0098791 GO:1902476

KEGG Pathways

Metabolic & Signaling

ko04966 map04966

Pfam Domains

Protein Families

AA_permease SLC12

Protein Analysis

984

Amino Acids

108.04

Weight (kDa)

6.89

Isoelectric Point (pI)

35.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease	PF00324	144 - 609	3.3e-54	Amino acid permease
AA_permease_2	PF13520	154 - 590	2.5e-16	Amino acid permease
SLC12	PF03522	651 - 772	8.3e-13	Solute carrier family 12
SLC12	PF03522	783 - 982	4.6e-26	Solute carrier family 12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30450 AT1G30450 AT1G30450 AT1G30450
fragaria_vesca	FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910
malus_domestica	MD05G1275000.v1.1 MD10G1254500.v1.1
prunus_persica	Prupe.4G088900_v2.0.a1 Prupe.4G088900_v2.0.a1
pyrus_communis	pycom05g25640 pycom10g21010
rosa_chinensis	RchiOBHm_Chr5g0015611 RchiOBHm_Chr5g0015801 RchiOBHm_Chr5g0015811
rosa_laevigata	RLG00000032213 RLG00000032223
rosa_multiflora	Rmu_co8164906.1_g000001 Rmu_sc0003989.1_g000002 Rmu_sc0005803.1_g000020 Rmu_sc0007629.1_g000001 Rmu_sc0011313.1_g000001 Rmu_sc0011313.1_g000002 Rmu_sc0016973.1_g000001
rosa_roxburghii	Rroxscaffold_1G00060860
rosa_rugosa	Rorug05G0028900
rosa_samantha	Rh5AG122200 Rh5BG120800 Rh5CG131200 Rh5CG132900 Rh5CG133000 Rh5DG119800 Rh5DG120900
rosa_wichuraiana	Rw5G010540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1044
AarI	CACCTGC	2 cut(s)	916, 2728
AatII	GACGTC	1 cut(s)	18
Acc36I	ACCTGC	4 cut(s)	834, 916, 1139, 2728
Acc65I	GGTACC	1 cut(s)	2357
AccB1I	GGYRCC	2 cut(s)	1394, 2357
AccB7I	CCANNNNNTGG	2 cut(s)	653, 2170
AccI	GTMKAC	2 cut(s)	12, 78
AclWI	GGATC	6 cut(s)	110, 123, 709, 1067, 1548, 1623
AcoI	YGGCCR	3 cut(s)	46, 1913, 2838
AcsI	RAATTY	4 cut(s)	1065, 1096, 1652, 2247
AcuI	CTGAAG	5 cut(s)	335, 434, 2067, 2163, 2565
AcyI	GRCGYC	3 cut(s)	15, 1639, 2227
AfaI	GTAC	6 cut(s)	65, 553, 1872, 2359, 2372, 2870
AfiI	CCNNNNNNNGG	8 cut(s)	362, 653, 755, 989, 1918, 2170, 2428, 2701
AhdI	GACNNNNNGTC	1 cut(s)	2172
AjnI	CCWGG	7 cut(s)	159, 1091, 1274, 1536, 1746, 1866, 2454
AleI	CACNNNNGTG	1 cut(s)	1733
AloI	GAACNNNNNNTCC	2 cut(s)	23, 55
Alw21I	GWGCWC	4 cut(s)	99, 191, 1037, 2635
Alw26I	GTCTC	8 cut(s)	764, 1249, 1409, 1743, 2399, 2601, 2893, 2922
AlwI	GGATC	6 cut(s)	110, 123, 709, 1067, 1548, 1623
Ama87I	CYCGRG	2 cut(s)	98, 2794
ApaI	GGGCCC	1 cut(s)	254
ApeKI	GCWGC	8 cut(s)	341, 752, 1211, 1373, 1412, 1927, 2401, 2804
ApoI	RAATTY	4 cut(s)	1065, 1096, 1652, 2247
ArsI	GACNNNNNNTTYG	2 cut(s)	841, 873
AseI	ATTAAT	1 cut(s)	425
Asp700I	GAANNNNTTC	1 cut(s)	2148
Asp718I	GGTACC	1 cut(s)	2357
AspLEI	GCGC	2 cut(s)	581, 1504
AspS9I	GGNCC	8 cut(s)	90, 224, 250, 251, 614, 644, 2128, 2691
AsuC2I	CCSGG	1 cut(s)	152
AsuHPI	GGTGA	8 cut(s)	149, 377, 971, 1649, 1814, 2039, 2215, 2923
AvaI	CYCGRG	2 cut(s)	98, 2794
AvaII	GGWCC	3 cut(s)	614, 644, 2691
AxyI	CCTNAGG	1 cut(s)	1366
BaeGI	GKGCMC	4 cut(s)	254, 640, 1399, 1944
BalI	TGGCCA	1 cut(s)	48
BamHI	GGATCC	1 cut(s)	115
BanI	GGYRCC	2 cut(s)	1394, 2357
BanII	GRGCYC	4 cut(s)	254, 1037, 1267, 2499
BarI	GAAGNNNNNNTAC	2 cut(s)	2738, 2770
BbsI	GAAGAC	4 cut(s)	39, 135, 1055, 1838
Bbv12I	GWGCWC	4 cut(s)	99, 191, 1037, 2635
BbvI	GCAGC	8 cut(s)	353, 764, 1198, 1360, 1424, 1914, 2388, 2791
BceAI	ACGGC	3 cut(s)	77, 427, 2285
BcgI	CGANNNNNNTGC	4 cut(s)	8, 42, 934, 968
BciT130I	CCWGG	7 cut(s)	161, 1093, 1276, 1538, 1748, 1868, 2456
BciVI	GTATCC	5 cut(s)	1099, 1899, 2223, 2473, 2912
BclI	TGATCA	2 cut(s)	891, 1701
BcnI	CCSGG	1 cut(s)	152
BcoDI	GTCTC	8 cut(s)	764, 1249, 1409, 1743, 2399, 2601, 2893, 2922
BfaI	CTAG	6 cut(s)	357, 683, 1311, 1742, 1856, 2021
BfmI	CTRYAG	2 cut(s)	339, 984
BfuAI	ACCTGC	4 cut(s)	834, 916, 1139, 2728
BfuI	GTATCC	5 cut(s)	1099, 1899, 2223, 2473, 2912
BisI	GCNGC	9 cut(s)	342, 753, 1212, 1374, 1413, 1928, 2402, 2639, 2805
BlpI	GCTNAGC	1 cut(s)	2634
BlsI	GCNGC	9 cut(s)	343, 754, 1213, 1375, 1414, 1929, 2403, 2640, 2806
Bme1390I	CCNGG	8 cut(s)	152, 161, 1093, 1276, 1538, 1748, 1868, 2456
Bme18I	GGWCC	3 cut(s)	614, 644, 2691
BmeRI	GACNNNNNGTC	1 cut(s)	2172
BmeT110I	CYCGRG	2 cut(s)	98, 2794
BmgT120I	GGNCC	8 cut(s)	90, 224, 250, 251, 614, 644, 2128, 2691
BmrFI	CCNGG	8 cut(s)	152, 161, 1093, 1276, 1538, 1748, 1868, 2456
BmrI	ACTGGG	5 cut(s)	425, 1325, 1626, 1807, 2127
BmsI	GCATC	8 cut(s)	1615, 1785, 2041, 2166, 2196, 2475, 2476, 2526
BmuI	ACTGGG	5 cut(s)	425, 1325, 1626, 1807, 2127
BpiI	GAAGAC	4 cut(s)	39, 135, 1055, 1838
BplI	GAGNNNNNCTC	4 cut(s)	1657, 1689, 2812, 2844
BpmI	CTGGAG	2 cut(s)	1051, 1684
Bpu1102I	GCTNAGC	1 cut(s)	2634
BpuEI	CTTGAG	5 cut(s)	155, 1024, 2171, 2483, 2586
BpuMI	CCSGG	1 cut(s)	152
BsaHI	GRCGYC	3 cut(s)	15, 1639, 2227
BsaI	GGTCTC	4 cut(s)	764, 1249, 2399, 2601
BsaJI	CCNNGG	8 cut(s)	43, 111, 246, 640, 1275, 1643, 1746, 1916
BsaXI	ACNNNNNCTCC	2 cut(s)	1821, 1851
Bsc4I	CCNNNNNNNGG	8 cut(s)	362, 653, 755, 989, 1918, 2170, 2428, 2701
Bse118I	RCCGGY	4 cut(s)	23, 748, 1911, 2836
Bse21I	CCTNAGG	1 cut(s)	1366
Bse3DI	GCAATG	3 cut(s)	1113, 1239, 1482
BseBI	CCWGG	7 cut(s)	161, 1093, 1276, 1538, 1748, 1868, 2456
BseDI	CCNNGG	8 cut(s)	43, 111, 246, 640, 1275, 1643, 1746, 1916
BseGI	GGATG	7 cut(s)	334, 1630, 1893, 1948, 2541, 2581, 2734
BseLI	CCNNNNNNNGG	8 cut(s)	362, 653, 755, 989, 1918, 2170, 2428, 2701
BseMI	GCAATG	3 cut(s)	1113, 1239, 1482
BseMII	CTCAG	9 cut(s)	398, 507, 1050, 1196, 2106, 2208, 2648, 2670, 2800
BseRI	GAGGAG	7 cut(s)	115, 118, 1496, 2408, 2411, 2489, 2589
BseSI	GKGCMC	4 cut(s)	254, 640, 1399, 1944
BseXI	GCAGC	8 cut(s)	353, 764, 1198, 1360, 1424, 1914, 2388, 2791
BseYI	CCCAGC	3 cut(s)	755, 1790, 2412
Bsh1285I	CGRYCG	1 cut(s)	88
BshNI	GGYRCC	2 cut(s)	1394, 2357
BsiEI	CGRYCG	1 cut(s)	88
BsiHKAI	GWGCWC	4 cut(s)	99, 191, 1037, 2635
BsiHKCI	CYCGRG	2 cut(s)	98, 2794
BsiSI	CCGG	5 cut(s)	24, 152, 749, 1912, 2837
BslFI	GGGAC	3 cut(s)	1808, 2395, 2704
BslI	CCNNNNNNNGG	8 cut(s)	362, 653, 755, 989, 1918, 2170, 2428, 2701
BsmAI	GTCTC	8 cut(s)	764, 1249, 1409, 1743, 2399, 2601, 2893, 2922
BsmBI	CGTCTC	2 cut(s)	1409, 2922
BsmFI	GGGAC	3 cut(s)	1808, 2395, 2704
Bso31I	GGTCTC	4 cut(s)	764, 1249, 2399, 2601
BsoBI	CYCGRG	2 cut(s)	98, 2794
Bsp120I	GGGCCC	1 cut(s)	250
Bsp1720I	GCTNAGC	1 cut(s)	2634
Bsp19I	CCATGG	2 cut(s)	111, 1916
BspCNI	CTCAG	9 cut(s)	397, 508, 1049, 1195, 2107, 2209, 2647, 2671, 2799
BspHI	TCATGA	1 cut(s)	820
BspMAI	CTGCAG	1 cut(s)	343
BspMI	ACCTGC	4 cut(s)	834, 916, 1139, 2728
BspPI	GGATC	6 cut(s)	110, 123, 709, 1067, 1548, 1623
BspQI	GCTCTTC	1 cut(s)	1383
BspT107I	GGYRCC	2 cut(s)	1394, 2357
BspTNI	GGTCTC	4 cut(s)	764, 1249, 2399, 2601
BsrDI	GCAATG	3 cut(s)	1113, 1239, 1482
BsrFI	RCCGGY	4 cut(s)	23, 748, 1911, 2836
BssAI	RCCGGY	4 cut(s)	23, 748, 1911, 2836
BssECI	CCNNGG	8 cut(s)	43, 111, 246, 640, 1275, 1643, 1746, 1916
BssNI	GRCGYC	3 cut(s)	15, 1639, 2227
BssT1I	CCWWGG	4 cut(s)	111, 246, 640, 1916
Bst2UI	CCWGG	7 cut(s)	161, 1093, 1276, 1538, 1748, 1868, 2456
Bst4CI	ACNGT	5 cut(s)	809, 1976, 2027, 2036, 2924
Bst6I	CTCTTC	4 cut(s)	136, 311, 1383, 1680
BstACI	GRCGYC	3 cut(s)	15, 1639, 2227
BstDSI	CCRYGG	3 cut(s)	43, 111, 1916
BstENI	CCTNNNNNAGG	1 cut(s)	2426
BstF5I	GGATG	7 cut(s)	334, 1630, 1893, 1948, 2541, 2581, 2734
BstHHI	GCGC	2 cut(s)	581, 1504
BstMAI	GTCTC	8 cut(s)	764, 1249, 1409, 1743, 2399, 2601, 2893, 2922
BstMCI	CGRYCG	1 cut(s)	88
BstNI	CCWGG	7 cut(s)	161, 1093, 1276, 1538, 1748, 1868, 2456
BstNSI	RCATGY	2 cut(s)	2067, 2543
BstSCI	CCNGG	8 cut(s)	150, 159, 1091, 1274, 1536, 1746, 1866, 2454
BstSFI	CTRYAG	2 cut(s)	339, 984
BstSLI	GKGCMC	4 cut(s)	254, 640, 1399, 1944
BstV1I	GCAGC	8 cut(s)	353, 764, 1198, 1360, 1424, 1914, 2388, 2791
BstV2I	GAAGAC	4 cut(s)	39, 135, 1055, 1838
BstX2I	RGATCY	4 cut(s)	115, 701, 1540, 1615
BstXI	CCANNNNNNTGG	1 cut(s)	1884
BstYI	RGATCY	4 cut(s)	115, 701, 1540, 1615
Bsu36I	CCTNAGG	1 cut(s)	1366
BsuI	GTATCC	5 cut(s)	1099, 1899, 2223, 2473, 2912
BtgI	CCRYGG	3 cut(s)	43, 111, 1916
BtgZI	GCGATG	1 cut(s)	2452
BtsCI	GGATG	7 cut(s)	334, 1630, 1893, 1948, 2541, 2581, 2734
BtsIMutI	CAGTG	4 cut(s)	1169, 1205, 1660, 2625
BveI	ACCTGC	4 cut(s)	834, 916, 1139, 2728
CciI	TCATGA	1 cut(s)	820
CfoI	GCGC	2 cut(s)	581, 1504
Cfr10I	RCCGGY	4 cut(s)	23, 748, 1911, 2836
Cfr13I	GGNCC	8 cut(s)	90, 224, 250, 251, 614, 644, 2128, 2691
CseI	GACGC	2 cut(s)	1628, 2216
CsiI	ACCWGGT	1 cut(s)	1866
Csp6I	GTAC	6 cut(s)	64, 552, 1871, 2358, 2371, 2869
CspCI	CAANNNNNGTGG	4 cut(s)	2181, 2216, 2245, 2280
CviQI	GTAC	6 cut(s)	64, 552, 1871, 2358, 2371, 2869
DriI	GACNNNNNGTC	1 cut(s)	2172
EaeI	YGGCCR	3 cut(s)	46, 1913, 2838
Eam1104I	CTCTTC	4 cut(s)	136, 311, 1383, 1680
Eam1105I	GACNNNNNGTC	1 cut(s)	2172
EarI	CTCTTC	4 cut(s)	136, 311, 1383, 1680
EciI	GGCGGA	1 cut(s)	2656
Ecl136II	GAGCTC	1 cut(s)	1035
Eco130I	CCWWGG	4 cut(s)	111, 246, 640, 1916
Eco24I	GRGCYC	4 cut(s)	254, 1037, 1267, 2499
Eco31I	GGTCTC	4 cut(s)	764, 1249, 2399, 2601
Eco47I	GGWCC	3 cut(s)	614, 644, 2691
Eco53kI	GAGCTC	1 cut(s)	1035
Eco57I	CTGAAG	5 cut(s)	335, 434, 2067, 2163, 2565
Eco81I	CCTNAGG	1 cut(s)	1366
Eco88I	CYCGRG	2 cut(s)	98, 2794
EcoICRI	GAGCTC	1 cut(s)	1035
EcoNI	CCTNNNNNAGG	1 cut(s)	2426
EcoO109I	RGGNCCY	4 cut(s)	224, 250, 251, 2691
EcoRI	GAATTC	1 cut(s)	1065
EcoRII	CCWGG	7 cut(s)	159, 1091, 1274, 1536, 1746, 1866, 2454
EcoT14I	CCWWGG	4 cut(s)	111, 246, 640, 1916
EcoT22I	ATGCAT	1 cut(s)	2541
EcoT38I	GRGCYC	4 cut(s)	254, 1037, 1267, 2499
ErhI	CCWWGG	4 cut(s)	111, 246, 640, 1916
Esp3I	CGTCTC	2 cut(s)	1409, 2922
FalI	AAGNNNNNCTT	2 cut(s)	2420, 2452
FaqI	GGGAC	3 cut(s)	1808, 2395, 2704
FauI	CCCGC	2 cut(s)	1579, 2703
FbaI	TGATCA	2 cut(s)	891, 1701
FblI	GTMKAC	2 cut(s)	12, 78
Fnu4HI	GCNGC	9 cut(s)	342, 753, 1212, 1374, 1413, 1928, 2402, 2639, 2805
FokI	GGATG	7 cut(s)	341, 1637, 1880, 1935, 2548, 2588, 2741
FriOI	GRGCYC	4 cut(s)	254, 1037, 1267, 2499
Fsp4HI	GCNGC	9 cut(s)	342, 753, 1212, 1374, 1413, 1928, 2402, 2639, 2805
FspBI	CTAG	6 cut(s)	357, 683, 1311, 1742, 1856, 2021
GlaI	GCGC	2 cut(s)	580, 1503
GluI	GCNGC	9 cut(s)	342, 753, 1212, 1374, 1413, 1928, 2402, 2639, 2805
GsaI	CCCAGC	3 cut(s)	759, 1794, 2416
GsuI	CTGGAG	2 cut(s)	1051, 1684
HapII	CCGG	5 cut(s)	24, 152, 749, 1912, 2837
HgaI	GACGC	2 cut(s)	1628, 2216
HhaI	GCGC	2 cut(s)	581, 1504
Hin1I	GRCGYC	3 cut(s)	15, 1639, 2227
Hin6I	GCGC	2 cut(s)	579, 1502
HinP1I	GCGC	2 cut(s)	579, 1502
HincII	GTYRAC	3 cut(s)	13, 79, 301
HindII	GTYRAC	3 cut(s)	13, 79, 301
HindIII	AAGCTT	2 cut(s)	1283, 2434
HinfI	GANTC	5 cut(s)	263, 290, 518, 976, 2609
HpaII	CCGG	5 cut(s)	24, 152, 749, 1912, 2837
HphI	GGTGA	8 cut(s)	149, 377, 971, 1649, 1814, 2039, 2215, 2923
Hpy99I	CGWCG	6 cut(s)	17, 20, 77, 80, 142, 2232
HpyCH4III	ACNGT	5 cut(s)	809, 1976, 2027, 2036, 2924
HpyCH4IV	ACGT	3 cut(s)	15, 2892, 2931
HpySE526I	ACGT	3 cut(s)	15, 2892, 2931
Hsp92I	GRCGYC	3 cut(s)	15, 1639, 2227
HspAI	GCGC	2 cut(s)	579, 1502
KflI	GGGWCCC	1 cut(s)	2691
KpnI	GGTACC	1 cut(s)	2361
KroI	GCCGGC	3 cut(s)	748, 1911, 2836
KroNI	GCCGGC	3 cut(s)	750, 1913, 2838
Ksp22I	TGATCA	2 cut(s)	891, 1701
LguI	GCTCTTC	1 cut(s)	1383
LmnI	GCTCC	9 cut(s)	719, 1032, 1262, 1509, 1633, 1665, 1682, 1859, 2421
Lsp1109I	GCAGC	8 cut(s)	353, 764, 1198, 1360, 1424, 1914, 2388, 2791
LweI	GCATC	8 cut(s)	1615, 1785, 2041, 2166, 2196, 2475, 2476, 2526
MabI	ACCWGGT	1 cut(s)	1866
MaeI	CTAG	6 cut(s)	357, 683, 1311, 1742, 1856, 2021
MaeII	ACGT	3 cut(s)	15, 2892, 2931
MaeIII	GTNAC	7 cut(s)	519, 847, 994, 1132, 1251, 2557, 2935
MfeI	CAATTG	1 cut(s)	582
MflI	RGATCY	4 cut(s)	115, 701, 1540, 1615
MlsI	TGGCCA	1 cut(s)	48
MluNI	TGGCCA	1 cut(s)	48
MlyI	GAGTC	1 cut(s)	527
MmeI	TCCRAC	2 cut(s)	634, 1852
Mox20I	TGGCCA	1 cut(s)	48
Mph1103I	ATGCAT	1 cut(s)	2541
MroNI	GCCGGC	3 cut(s)	748, 1911, 2836
MroXI	GAANNNNTTC	1 cut(s)	2148
MscI	TGGCCA	1 cut(s)	48
MseI	TTAA	5 cut(s)	425, 783, 1446, 2240, 2274
MslI	CAYNNNNRTG	2 cut(s)	1733, 2201
Msp20I	TGGCCA	1 cut(s)	48
MspA1I	CMGCKG	3 cut(s)	755, 2638, 2698
MspI	CCGG	5 cut(s)	24, 152, 749, 1912, 2837
MspR9I	CCNGG	8 cut(s)	152, 161, 1093, 1276, 1538, 1748, 1868, 2456
MunI	CAATTG	1 cut(s)	582
MvaI	CCWGG	7 cut(s)	161, 1093, 1276, 1538, 1748, 1868, 2456
NaeI	GCCGGC	3 cut(s)	750, 1913, 2838
NciI	CCSGG	1 cut(s)	152
NcoI	CCATGG	2 cut(s)	111, 1916
NgoMIV	GCCGGC	3 cut(s)	748, 1911, 2836
NmeAIII	GCCGAG	2 cut(s)	1286, 2922
NmuCI	GTSAC	4 cut(s)	519, 847, 994, 1132
NsiI	ATGCAT	1 cut(s)	2541
NspI	RCATGY	2 cut(s)	2067, 2543
OliI	CACNNNNGTG	1 cut(s)	1733
PaeI	GCATGC	2 cut(s)	2067, 2543
PaeR7I	CTCGAG	2 cut(s)	98, 2794
PagI	TCATGA	1 cut(s)	820
PaqCI	CACCTGC	2 cut(s)	916, 2728
PciSI	GCTCTTC	1 cut(s)	1383
PcsI	WCGNNNNNNNCGW	2 cut(s)	81, 90
PdiI	GCCGGC	3 cut(s)	750, 1913, 2838
PdmI	GAANNNNTTC	1 cut(s)	2148
PfeI	GAWTC	4 cut(s)	263, 290, 976, 2609
PflFI	GACNNNGTC	1 cut(s)	8
PflMI	CCANNNNNTGG	2 cut(s)	653, 2170
PfoI	TCCNGGA	1 cut(s)	1091
PkrI	GCNGC	9 cut(s)	343, 754, 1213, 1375, 1414, 1929, 2403, 2640, 2806
Ple19I	CGATCG	1 cut(s)	88
PleI	GAGTC	1 cut(s)	526
PpsI	GAGTC	1 cut(s)	526
PpuMI	RGGWCCY	1 cut(s)	2691
PshBI	ATTAAT	1 cut(s)	425
PsiI	TTATAA	1 cut(s)	1044
Psp124BI	GAGCTC	1 cut(s)	1037
Psp5II	RGGWCCY	1 cut(s)	2691
Psp6I	CCWGG	7 cut(s)	159, 1091, 1274, 1536, 1746, 1866, 2454
PspFI	CCCAGC	3 cut(s)	755, 1790, 2412
PspGI	CCWGG	7 cut(s)	159, 1091, 1274, 1536, 1746, 1866, 2454
PspOMI	GGGCCC	1 cut(s)	250
PspPI	GGNCC	8 cut(s)	90, 224, 250, 251, 614, 644, 2128, 2691
PspPPI	RGGWCCY	1 cut(s)	2691
PsrI	GAACNNNNNNTAC	2 cut(s)	781, 813
PstI	CTGCAG	1 cut(s)	343
PsuI	RGATCY	4 cut(s)	115, 701, 1540, 1615
PsyI	GACNNNGTC	1 cut(s)	8
PvuI	CGATCG	1 cut(s)	88
PvuII	CAGCTG	1 cut(s)	755
RsaI	GTAC	6 cut(s)	65, 553, 1872, 2359, 2372, 2870
RsaNI	GTAC	6 cut(s)	64, 552, 1871, 2358, 2371, 2869
RseI	CAYNNNNRTG	2 cut(s)	1733, 2201
SacI	GAGCTC	1 cut(s)	1037
SalI	GTCGAC	2 cut(s)	11, 77
SapI	GCTCTTC	1 cut(s)	1383
SaqAI	TTAA	5 cut(s)	425, 783, 1446, 2240, 2274
SatI	GCNGC	9 cut(s)	342, 753, 1212, 1374, 1413, 1928, 2402, 2639, 2805
Sau96I	GGNCC	8 cut(s)	90, 224, 250, 251, 614, 644, 2128, 2691
SchI	GAGTC	1 cut(s)	527
ScrFI	CCNGG	8 cut(s)	152, 161, 1093, 1276, 1538, 1748, 1868, 2456
SexAI	ACCWGGT	1 cut(s)	1866
SfaNI	GCATC	8 cut(s)	1615, 1785, 2041, 2166, 2196, 2475, 2476, 2526
SfcI	CTRYAG	2 cut(s)	339, 984
Sfr274I	CTCGAG	2 cut(s)	98, 2794
SinI	GGWCC	3 cut(s)	614, 644, 2691
SlaI	CTCGAG	2 cut(s)	98, 2794
SmiMI	CAYNNNNRTG	2 cut(s)	1733, 2201
SmlI	CTYRAG	7 cut(s)	98, 170, 1003, 2186, 2498, 2601, 2794
SmoI	CTYRAG	7 cut(s)	98, 170, 1003, 2186, 2498, 2601, 2794
SphI	GCATGC	2 cut(s)	2067, 2543
SspMI	CTAG	6 cut(s)	357, 683, 1311, 1742, 1856, 2021
SstI	GAGCTC	1 cut(s)	1037
StyD4I	CCNGG	8 cut(s)	150, 159, 1091, 1274, 1536, 1746, 1866, 2454
StyI	CCWWGG	4 cut(s)	111, 246, 640, 1916
TaaI	ACNGT	5 cut(s)	809, 1976, 2027, 2036, 2924
TaiI	ACGT	3 cut(s)	18, 2895, 2934
TaqI	TCGA	7 cut(s)	12, 18, 78, 99, 1849, 2777, 2795
TaqII	GACCGA	1 cut(s)	1149
TatI	WGTACW	3 cut(s)	551, 2370, 2868
TauI	GCSGC	1 cut(s)	2641
TfiI	GAWTC	4 cut(s)	263, 290, 976, 2609
Tru1I	TTAA	5 cut(s)	425, 783, 1446, 2240, 2274
Tru9I	TTAA	5 cut(s)	425, 783, 1446, 2240, 2274
TscAI	CASTG	4 cut(s)	1176, 1212, 1667, 2632
TseFI	GTSAC	4 cut(s)	519, 847, 994, 1132
TseI	GCWGC	8 cut(s)	341, 752, 1211, 1373, 1412, 1927, 2401, 2804
Tsp45I	GTSAC	4 cut(s)	519, 847, 994, 1132
TspDTI	ATGAA	9 cut(s)	234, 617, 1327, 1994, 2340, 2579, 2622, 2681, 2745
TspGWI	ACGGA	4 cut(s)	32, 341, 1095, 2258
TspRI	CASTG	4 cut(s)	1176, 1212, 1667, 2632
Tth111I	GACNNNGTC	1 cut(s)	8
Van91I	CCANNNNNTGG	2 cut(s)	653, 2170
VpaK11BI	GGWCC	3 cut(s)	614, 644, 2691
VspI	ATTAAT	1 cut(s)	425
XagI	CCTNNNNNAGG	1 cut(s)	2426
XapI	RAATTY	4 cut(s)	1065, 1096, 1652, 2247
XbaI	TCTAGA	1 cut(s)	2020
XceI	RCATGY	2 cut(s)	2067, 2543
XhoI	CTCGAG	2 cut(s)	98, 2794
XmiI	GTMKAC	2 cut(s)	12, 78
XmnI	GAANNNNTTC	1 cut(s)	2148
XspI	CTAG	6 cut(s)	357, 683, 1311, 1742, 1856, 2021
ZraI	GACGTC	1 cut(s)	16
Zsp2I	ATGCAT	1 cut(s)	2541

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.