Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0003023

Physical Location & Seq

Genomic Coordinates Reverse (-)

26969294 .. 26978473

Gene Structure 9180 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g25640.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2547 bp

ATGGACAATGCCGACGTCGAAGCCGGTGCGGAAGAGGAGTTCCGTGGCCAGAGGGGTCGCAAGTATCGCCCCGTCGTCGACGACGATCGCGCCGTGCTCGAGATGTCGTCCATGGATCCTTCCTCCTCCTCCTCTTCCTCATCTTCTCTCCCCGGCCACCAAGCTTCACTCCAGAAAATAAAAGTGAGCACCCAAGAAAACATGGGTTCTAACGTACAAGAAGGGCCTTCTACTCATGTGCAAGCCAATGGGCCCCAGAAAGAATCAAAATTGGAACTATTTGGTTTTGATTCTCTCGTCAACATTCTTGGTCTGAAGAGTATGACAGATGAGCAGACTGCAGCACCGTCAAGTCCTAGAGATGGTGAAGATATTGCTATCACTCAGGGGCGACCAAAGCCAAGTGATCTCAAATTGGGAACAATGATGGGTGTATTTGTGCCATGCCTTCAAAACATATTGGGAATTATCTACTATATCCGCTTTTCTTGGATTGTTGGTATGGCTGGCATAGCTGAGTCACTACTCTTGGTTTTCTTCTGTGGCTTGTGTACTTTCCTGACTTCAATATCATTGAGTGCAATTGCAACCAATGGTGCTATGAAGGGTGGTGGACCTTACTATCTAATTGGTCGTGCCCTTGGTCCAGAAGTTGGAGTTAGCATTGGATTATGTTTCTTTCTAGGAAATGCTGTTGCTCGATCTCTTTATGTCTTGGGAGCTGTTGAAACCTTCTTGAAAGCTGTACCAGCAGCTGGGATTTTTAGAGAGACCACAAGAGTTAATGGAACATCAATTCAAATACAAACTCCGAGTTCACATGACCTACAAATCTATGGGATAGTTGTAACTATTCTTTTGTGCTTTATTGTGTTTGGTGGTGTGAAAATGATCAATCGGGTTGCACCTGCTTTCCTCATACCTGTTTTATTCTCGCTGTTCTGCATATATATCGGGATTGCTTTGGCGAGGAAGAATCACCCTGTAGATGGGGTCACGGGCTTGAGTTTGAACTCTTTTAAAGAAAACTGGAGCTCAGATTATCAGAAGACCAATAACAATGGAATCCCTGATCCTGACGGAAAAGTATCCTGGAATTTCAATGCAATGGTCGGCCTCTTTTTCCCTGCTGTGACAGGAATTATGGCAGGTTCAAATCGGTCAGCCTCACTAAAAGATACTCAGCGTTCAATTCCTATTGGAACACTGGCTGCAACTCTTACAACTACGGCAATGTATCTGGTCTCTGTGTTACTATTTGGAGCCCTTGCAACCAGGGAGAAGCTTTTGACTGACAGGCTACTTTCGGCTACAATAGCTTGGCCTTTCCCAGTATTCATTTACATTGGAATAATTCTTTCAACCTTAGGTGCTGCTCTTCAAAGCCTGACTGGTGCCCCCCGTCTCCTTGCAGCAATAGCCAATGATGACATTTTACCTGTTCTTAACTACTTCCGGGTTTCAGAGGGGAATGAGCCTCACATTGCTACCTTGTTTACAGCGCTCCTCTGTATCGGGTGTGTCGTTATTGGGAACCTGGATCGAATCACACCAACTATAACTATGTTTTTCCTTCTGTGTTATGCGGGTGTGAACTTATCTTGCTTCCTTCTGGATCTTCTAGATGCTCCCAGTTGGCGTCCTCGGTGGAAATTTCACCACTGGAGCCTCTCTCTTCTTGGAGCCTCACTTTGTATAGTGATCATGTTCTTGATCTCCTGGACATTCACTATTGTGTCTCTAGCCCTGGCAAGCCTTATTTATTATTATGTAAGCATCAAGGGCAAGGCTGGGGATTGGGGTGATGGTTTCAAGAGTGCATATTTCCAACTGGCTCTCCGCAGTCTACGATCTCTAGGAGCAAACCAGGTGCACCCAAAGAATTGGTACCCCATCCCATTGATATTCTGCCGGCCATGGGGGAAGCTGCCAGATAATGTACCCTGCCATCCCAAACTTGCTGACTTTGCCAACTGTATGAAGAAGAAGGGCAGGGGAATGTCCATCTTTTTTGCTATTATGGACGGTGATTACCGTGAATGTGCTGAAGATGCTAAGACTGCATGCAAACAGCTTGCTACCTACCTTGACTACAAGAATTGTGAAGGTGTAGCTGAGATTGTCGTGGCCCCAAGTGTGTCTGAAGGATTTCGGGGCATTGTCCAGACAATGGGTCTTGGAAACCTCAAGCCAAACATTGTGGTGATGCGGTATCCTGAGATATGGCGTCGTGAAAACTTAACGGAAATTCCAGCCACTTTCGTTGAAATAATTAACGATTGCATTGTTGCGAACAAGGCTGTTGTTATTGTCAAGGGTCTTGACGAGTGGCCTAATGAGTATCAGAGGCAGTATGGTACCATTGACTTATATTGGATAGTGAGAGATGGGGGTCTCATGCTTCTTCTCTCTCAACTCCTCCTCACGAAGGAAAGCTTTGAGAGTTGCAAAATCCAGGTTTTCTGCATCGCGGAGGAGGATACTGATGCGGAGGGGCTCAAGGCTGATGTCAAGAAGTTTCTGTATCGATCTTCGGATGCATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006820 GO:0006821 GO:0008150 GO:0008324 GO:0008509 GO:0008511 GO:0009674 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015103 GO:0015108 GO:0015291 GO:0015293 GO:0015294 GO:0015296 GO:0015318 GO:0015370 GO:0015373 GO:0015377 GO:0015378 GO:0015379 GO:0015672 GO:0015698 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0034220 GO:0035725 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055064 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055081 GO:0055083 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097708 GO:0098655 GO:0098656 GO:0098660 GO:0098661 GO:0098662 GO:0098771 GO:0098791 GO:1902476

KEGG Pathways

Metabolic & Signaling

ko04966 map04966

Pfam Domains

Protein Families

AA_permease SLC12

Protein Analysis

849

Amino Acids

92.75

Weight (kDa)

6.76

Isoelectric Point (pI)

32.3

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30450 AT1G30450 AT1G30450 AT1G30450
fragaria_vesca	FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910
malus_domestica	MD05G1275000.v1.1 MD10G1254500.v1.1
prunus_persica	Prupe.4G088900_v2.0.a1 Prupe.4G088900_v2.0.a1
pyrus_communis	pycom05g25640 pycom10g21010
rosa_chinensis	RchiOBHm_Chr5g0015611 RchiOBHm_Chr5g0015801 RchiOBHm_Chr5g0015811
rosa_laevigata	RLG00000032213 RLG00000032223
rosa_multiflora	Rmu_co8164906.1_g000001 Rmu_sc0003989.1_g000002 Rmu_sc0005803.1_g000020 Rmu_sc0007629.1_g000001 Rmu_sc0011313.1_g000001 Rmu_sc0011313.1_g000002 Rmu_sc0016973.1_g000001
rosa_roxburghii	Rroxscaffold_1G00060860
rosa_rugosa	Rorug05G0028900
rosa_samantha	Rh5AG122200 Rh5BG120800 Rh5CG131200 Rh5CG132900 Rh5CG133000 Rh5DG119800 Rh5DG120900
rosa_wichuraiana	Rw5G010540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	916
AatII	GACGTC	1 cut(s)	18
Acc36I	ACCTGC	2 cut(s)	916, 1139
Acc65I	GGTACC	2 cut(s)	1887, 2357
AccB1I	GGYRCC	3 cut(s)	1394, 1887, 2357
AccB7I	CCANNNNNTGG	3 cut(s)	653, 755, 2170
AccI	GTMKAC	2 cut(s)	78, 1846
AccII	CGCG	2 cut(s)	90, 2471
AciI	CCGC	7 cut(s)	29, 481, 1586, 1840, 2209, 2471, 2489
AclWI	GGATC	5 cut(s)	110, 123, 1067, 1548, 1623
AcoI	YGGCCR	3 cut(s)	46, 154, 1913
AcsI	RAATTY	3 cut(s)	1096, 1652, 2247
AcuI	CTGAAG	3 cut(s)	335, 2067, 2163
AcyI	GRCGYC	3 cut(s)	15, 1639, 2227
AfaI	GTAC	6 cut(s)	216, 553, 747, 1889, 1941, 2359
AfeI	AGCGCT	1 cut(s)	1503
AfiI	CCNNNNNNNGG	7 cut(s)	362, 653, 755, 989, 1918, 2170, 2428
AhdI	GACNNNNNGTC	1 cut(s)	2172
AjnI	CCWGG	7 cut(s)	1091, 1274, 1536, 1718, 1746, 1866, 2454
AleI	CACNNNNGTG	1 cut(s)	1733
AloI	GAACNNNNNNTCC	2 cut(s)	23, 55
Alw21I	GWGCWC	4 cut(s)	99, 191, 1037, 1875
Alw26I	GTCTC	5 cut(s)	764, 1249, 1409, 1743, 2399
Alw44I	GTGCAC	1 cut(s)	1871
AlwI	GGATC	5 cut(s)	110, 123, 1067, 1548, 1623
AlwNI	CAGNNNCTG	1 cut(s)	755
Ama87I	CYCGRG	1 cut(s)	98
Aor51HI	AGCGCT	1 cut(s)	1503
AoxI	GGCC	9 cut(s)	46, 154, 224, 251, 1114, 1322, 1913, 2127, 2330
ApaI	GGGCCC	1 cut(s)	255
ApaLI	GTGCAC	1 cut(s)	1871
ApeKI	GCWGC	6 cut(s)	341, 752, 1211, 1373, 1412, 1927
ApoI	RAATTY	3 cut(s)	1096, 1652, 2247
Asp700I	GAANNNNTTC	1 cut(s)	2148
Asp718I	GGTACC	2 cut(s)	1887, 2357
AspLEI	GCGC	2 cut(s)	92, 1504
AspS9I	GGNCC	6 cut(s)	224, 251, 252, 614, 644, 2128
AsuC2I	CCSGG	2 cut(s)	153, 1457
AsuHPI	GGTGA	6 cut(s)	377, 971, 1649, 1814, 2039, 2215
AvaI	CYCGRG	1 cut(s)	98
AvaII	GGWCC	2 cut(s)	614, 644
AxyI	CCTNAGG	1 cut(s)	1366
BaeGI	GKGCMC	4 cut(s)	255, 640, 1399, 1875
BalI	TGGCCA	1 cut(s)	48
BamHI	GGATCC	1 cut(s)	115
BanI	GGYRCC	3 cut(s)	1394, 1887, 2357
BanII	GRGCYC	4 cut(s)	255, 1037, 1267, 2499
BbsI	GAAGAC	1 cut(s)	1055
Bbv12I	GWGCWC	4 cut(s)	99, 191, 1037, 1875
BbvI	GCAGC	6 cut(s)	353, 764, 1198, 1360, 1424, 1914
BccI	CCATC	8 cut(s)	356, 421, 983, 1799, 1901, 1956, 2012, 2381
BceAI	ACGGC	2 cut(s)	77, 1245
BcgI	CGANNNNNNTGC	4 cut(s)	8, 42, 934, 968
BciT130I	CCWGG	7 cut(s)	1093, 1276, 1538, 1720, 1748, 1868, 2456
BciVI	GTATCC	3 cut(s)	1099, 2223, 2473
BclI	TGATCA	2 cut(s)	891, 1701
BcnI	CCSGG	2 cut(s)	153, 1457
BcoDI	GTCTC	5 cut(s)	764, 1249, 1409, 1743, 2399
BfaI	CTAG	5 cut(s)	357, 683, 1622, 1742, 1856
BfmI	CTRYAG	2 cut(s)	339, 984
BfoI	RGCGCY	1 cut(s)	1505
BfuAI	ACCTGC	2 cut(s)	916, 1139
BfuI	GTATCC	3 cut(s)	1099, 2223, 2473
BisI	GCNGC	6 cut(s)	342, 753, 1212, 1374, 1413, 1928
BlsI	GCNGC	6 cut(s)	343, 754, 1213, 1375, 1414, 1929
Bme1390I	CCNGG	9 cut(s)	153, 1093, 1276, 1457, 1538, 1720, 1748, 1868, 2456
Bme18I	GGWCC	2 cut(s)	614, 644
BmeRI	GACNNNNNGTC	1 cut(s)	2172
BmeT110I	CYCGRG	1 cut(s)	98
BmgT120I	GGNCC	6 cut(s)	224, 251, 252, 614, 644, 2128
BmrFI	CCNGG	9 cut(s)	153, 1093, 1276, 1457, 1538, 1720, 1748, 1868, 2456
BmrI	ACTGGG	2 cut(s)	1325, 1626
BmsI	GCATC	7 cut(s)	1615, 1785, 2041, 2196, 2475, 2476, 2527
BmuI	ACTGGG	2 cut(s)	1325, 1626
BpiI	GAAGAC	1 cut(s)	1055
BplI	GAGNNNNNCTC	2 cut(s)	1657, 1689
BpmI	CTGGAG	3 cut(s)	155, 1051, 1684
BpuEI	CTTGAG	3 cut(s)	1024, 2171, 2483
BpuMI	CCSGG	2 cut(s)	153, 1457
Bsa29I	ATCGAT	1 cut(s)	2527
BsaHI	GRCGYC	3 cut(s)	15, 1639, 2227
BsaI	GGTCTC	3 cut(s)	764, 1249, 2399
BsaJI	CCNNGG	8 cut(s)	43, 111, 151, 640, 1275, 1643, 1746, 1916
BsaXI	ACNNNNNCTCC	2 cut(s)	1821, 1851
Bsc4I	CCNNNNNNNGG	7 cut(s)	362, 653, 755, 989, 1918, 2170, 2428
Bse118I	RCCGGY	2 cut(s)	23, 1911
Bse1I	ACTGG	7 cut(s)	1034, 1212, 1331, 1396, 1632, 1667, 1836
Bse21I	CCTNAGG	1 cut(s)	1366
Bse3DI	GCAATG	3 cut(s)	1113, 1239, 1482
BseBI	CCWGG	7 cut(s)	1093, 1276, 1538, 1720, 1748, 1868, 2456
BseCI	ATCGAT	1 cut(s)	2527
BseDI	CCNNGG	8 cut(s)	43, 111, 151, 640, 1275, 1643, 1746, 1916
BseGI	GGATG	3 cut(s)	1893, 1948, 2542
BseLI	CCNNNNNNNGG	7 cut(s)	362, 653, 755, 989, 1918, 2170, 2428
BseMI	GCAATG	3 cut(s)	1113, 1239, 1482
BseMII	CTCAG	6 cut(s)	398, 507, 1050, 1196, 2106, 2208
BseNI	ACTGG	7 cut(s)	1034, 1212, 1331, 1396, 1632, 1667, 1836
BseRI	GAGGAG	8 cut(s)	50, 115, 118, 121, 1496, 2408, 2411, 2489
BseSI	GKGCMC	4 cut(s)	255, 640, 1399, 1875
BseXI	GCAGC	6 cut(s)	353, 764, 1198, 1360, 1424, 1914
BseYI	CCCAGC	2 cut(s)	755, 1790
Bsh1236I	CGCG	2 cut(s)	90, 2471
Bsh1285I	CGRYCG	1 cut(s)	88
BshFI	GGCC	9 cut(s)	48, 156, 226, 253, 1116, 1324, 1915, 2129, 2332
BshNI	GGYRCC	3 cut(s)	1394, 1887, 2357
BshVI	ATCGAT	1 cut(s)	2527
BsiEI	CGRYCG	1 cut(s)	88
BsiHKAI	GWGCWC	4 cut(s)	99, 191, 1037, 1875
BsiHKCI	CYCGRG	1 cut(s)	98
BsiSI	CCGG	4 cut(s)	24, 153, 1456, 1912
BslI	CCNNNNNNNGG	7 cut(s)	362, 653, 755, 989, 1918, 2170, 2428
BsmAI	GTCTC	5 cut(s)	764, 1249, 1409, 1743, 2399
BsmBI	CGTCTC	1 cut(s)	1409
BsnI	GGCC	9 cut(s)	48, 156, 226, 253, 1116, 1324, 1915, 2129, 2332
Bso31I	GGTCTC	3 cut(s)	764, 1249, 2399
BsoBI	CYCGRG	1 cut(s)	98
Bsp120I	GGGCCC	1 cut(s)	251
Bsp1286I	GDGCHC	9 cut(s)	99, 191, 255, 640, 1037, 1267, 1399, 1875, 2499
Bsp19I	CCATGG	2 cut(s)	111, 1916
BspACI	CCGC	7 cut(s)	29, 481, 1586, 1840, 2209, 2471, 2489
BspANI	GGCC	9 cut(s)	48, 156, 226, 253, 1116, 1324, 1915, 2129, 2332
BspCNI	CTCAG	6 cut(s)	397, 508, 1049, 1195, 2107, 2209
BspDI	ATCGAT	1 cut(s)	2527
BspFNI	CGCG	2 cut(s)	90, 2471
BspMAI	CTGCAG	1 cut(s)	343
BspMI	ACCTGC	2 cut(s)	916, 1139
BspPI	GGATC	5 cut(s)	110, 123, 1067, 1548, 1623
BspQI	GCTCTTC	1 cut(s)	1383
BspT107I	GGYRCC	3 cut(s)	1394, 1887, 2357
BspTNI	GGTCTC	3 cut(s)	764, 1249, 2399
BsrDI	GCAATG	3 cut(s)	1113, 1239, 1482
BsrFI	RCCGGY	2 cut(s)	23, 1911
BsrI	ACTGG	7 cut(s)	1034, 1212, 1331, 1396, 1632, 1667, 1836
BssAI	RCCGGY	2 cut(s)	23, 1911
BssECI	CCNNGG	8 cut(s)	43, 111, 151, 640, 1275, 1643, 1746, 1916
BssNI	GRCGYC	3 cut(s)	15, 1639, 2227
BssT1I	CCWWGG	3 cut(s)	111, 640, 1916
Bst2UI	CCWGG	7 cut(s)	1093, 1276, 1538, 1720, 1748, 1868, 2456
Bst4CI	ACNGT	4 cut(s)	348, 1976, 2027, 2036
Bst6I	CTCTTC	5 cut(s)	27, 139, 311, 1383, 1680
BstACI	GRCGYC	3 cut(s)	15, 1639, 2227
BstC8I	GCNNGC	7 cut(s)	243, 508, 1753, 1913, 2065, 2076, 2542
BstDEI	CTNAG	8 cut(s)	384, 516, 1036, 1182, 1366, 2055, 2115, 2217
BstDSI	CCRYGG	3 cut(s)	43, 111, 1916
BstENI	CCTNNNNNAGG	1 cut(s)	2426
BstF5I	GGATG	3 cut(s)	1893, 1948, 2542
BstFNI	CGCG	2 cut(s)	90, 2471
BstH2I	RGCGCY	1 cut(s)	1505
BstHHI	GCGC	2 cut(s)	92, 1504
BstMAI	GTCTC	5 cut(s)	764, 1249, 1409, 1743, 2399
BstMCI	CGRYCG	1 cut(s)	88
BstNI	CCWGG	7 cut(s)	1093, 1276, 1538, 1720, 1748, 1868, 2456
BstNSI	RCATGY	2 cut(s)	2067, 2544
BstSCI	CCNGG	9 cut(s)	151, 1091, 1274, 1455, 1536, 1718, 1746, 1866, 2454
BstSFI	CTRYAG	2 cut(s)	339, 984
BstSLI	GKGCMC	4 cut(s)	255, 640, 1399, 1875
BstUI	CGCG	2 cut(s)	90, 2471
BstV1I	GCAGC	6 cut(s)	353, 764, 1198, 1360, 1424, 1914
BstV2I	GAAGAC	1 cut(s)	1055
BstX2I	RGATCY	2 cut(s)	115, 1615
BstXI	CCANNNNNNTGG	1 cut(s)	1884
BstYI	RGATCY	2 cut(s)	115, 1615
Bsu15I	ATCGAT	1 cut(s)	2527
Bsu36I	CCTNAGG	1 cut(s)	1366
BsuI	GTATCC	3 cut(s)	1099, 2223, 2473
BsuRI	GGCC	9 cut(s)	48, 156, 226, 253, 1116, 1324, 1915, 2129, 2332
BsuTUI	ATCGAT	1 cut(s)	2527
BtgI	CCRYGG	3 cut(s)	43, 111, 1916
BtgZI	GCGATG	1 cut(s)	2452
BtsCI	GGATG	3 cut(s)	1893, 1948, 2542
BtsIMutI	CAGTG	2 cut(s)	1205, 1660
BveI	ACCTGC	2 cut(s)	916, 1139
Cac8I	GCNNGC	7 cut(s)	243, 508, 1753, 1913, 2065, 2076, 2542
CaiI	CAGNNNCTG	1 cut(s)	755
CfoI	GCGC	2 cut(s)	92, 1504
Cfr10I	RCCGGY	2 cut(s)	23, 1911
Cfr13I	GGNCC	6 cut(s)	224, 251, 252, 614, 644, 2128
ClaI	ATCGAT	1 cut(s)	2527
CseI	GACGC	2 cut(s)	1628, 2216
CsiI	ACCWGGT	1 cut(s)	1866
Csp6I	GTAC	6 cut(s)	215, 552, 746, 1888, 1940, 2358
CspCI	CAANNNNNGTGG	4 cut(s)	2181, 2216, 2245, 2280
CviQI	GTAC	6 cut(s)	215, 552, 746, 1888, 1940, 2358
DdeI	CTNAG	8 cut(s)	384, 516, 1036, 1182, 1366, 2055, 2115, 2217
DraI	TTTAAA	1 cut(s)	1021
DriI	GACNNNNNGTC	1 cut(s)	2172
EaeI	YGGCCR	3 cut(s)	46, 154, 1913
Eam1104I	CTCTTC	5 cut(s)	27, 139, 311, 1383, 1680
Eam1105I	GACNNNNNGTC	1 cut(s)	2172
EarI	CTCTTC	5 cut(s)	27, 139, 311, 1383, 1680
Ecl136II	GAGCTC	1 cut(s)	1035
Eco130I	CCWWGG	3 cut(s)	111, 640, 1916
Eco24I	GRGCYC	4 cut(s)	255, 1037, 1267, 2499
Eco31I	GGTCTC	3 cut(s)	764, 1249, 2399
Eco47I	GGWCC	2 cut(s)	614, 644
Eco47III	AGCGCT	1 cut(s)	1503
Eco53kI	GAGCTC	1 cut(s)	1035
Eco57I	CTGAAG	3 cut(s)	335, 2067, 2163
Eco81I	CCTNAGG	1 cut(s)	1366
Eco88I	CYCGRG	1 cut(s)	98
EcoICRI	GAGCTC	1 cut(s)	1035
EcoNI	CCTNNNNNAGG	1 cut(s)	2426
EcoO109I	RGGNCCY	2 cut(s)	224, 252
EcoRII	CCWGG	7 cut(s)	1091, 1274, 1536, 1718, 1746, 1866, 2454
EcoT14I	CCWWGG	3 cut(s)	111, 640, 1916
EcoT22I	ATGCAT	1 cut(s)	2542
EcoT38I	GRGCYC	4 cut(s)	255, 1037, 1267, 2499
ErhI	CCWWGG	3 cut(s)	111, 640, 1916
Esp3I	CGTCTC	1 cut(s)	1409
FalI	AAGNNNNNCTT	2 cut(s)	2420, 2452
FauI	CCCGC	1 cut(s)	1579
FbaI	TGATCA	2 cut(s)	891, 1701
FblI	GTMKAC	2 cut(s)	78, 1846
Fnu4HI	GCNGC	6 cut(s)	342, 753, 1212, 1374, 1413, 1928
FokI	GGATG	2 cut(s)	1880, 1935
FriOI	GRGCYC	4 cut(s)	255, 1037, 1267, 2499
Fsp4HI	GCNGC	6 cut(s)	342, 753, 1212, 1374, 1413, 1928
FspBI	CTAG	5 cut(s)	357, 683, 1622, 1742, 1856
GlaI	GCGC	2 cut(s)	91, 1503
GluI	GCNGC	6 cut(s)	342, 753, 1212, 1374, 1413, 1928
GsaI	CCCAGC	2 cut(s)	759, 1794
GsuI	CTGGAG	3 cut(s)	155, 1051, 1684
HaeII	RGCGCY	1 cut(s)	1505
HaeIII	GGCC	9 cut(s)	48, 156, 226, 253, 1116, 1324, 1915, 2129, 2332
HapII	CCGG	4 cut(s)	24, 153, 1456, 1912
HgaI	GACGC	2 cut(s)	1628, 2216
HhaI	GCGC	2 cut(s)	92, 1504
Hin1I	GRCGYC	3 cut(s)	15, 1639, 2227
Hin6I	GCGC	2 cut(s)	90, 1502
HinP1I	GCGC	2 cut(s)	90, 1502
HincII	GTYRAC	2 cut(s)	79, 301
HindII	GTYRAC	2 cut(s)	79, 301
HindIII	AAGCTT	3 cut(s)	162, 1283, 2434
HinfI	GANTC	6 cut(s)	263, 290, 518, 976, 1065, 1545
HpaII	CCGG	4 cut(s)	24, 153, 1456, 1912
HphI	GGTGA	6 cut(s)	377, 971, 1649, 1814, 2039, 2215
Hpy166II	GTNNAC	9 cut(s)	79, 301, 552, 614, 818, 1497, 1594, 1847, 1873
Hpy188I	TCNGA	8 cut(s)	315, 813, 1039, 1047, 1465, 2143, 2346, 2536
Hpy8I	GTNNAC	9 cut(s)	79, 301, 552, 614, 818, 1497, 1594, 1847, 1873
Hpy99I	CGWCG	7 cut(s)	17, 20, 77, 80, 83, 86, 2232
HpyCH4III	ACNGT	4 cut(s)	348, 1976, 2027, 2036
HpyCH4IV	ACGT	2 cut(s)	15, 213
HpyF3I	CTNAG	8 cut(s)	384, 516, 1036, 1182, 1366, 2055, 2115, 2217
HpySE526I	ACGT	2 cut(s)	15, 213
Hsp92I	GRCGYC	3 cut(s)	15, 1639, 2227
HspAI	GCGC	2 cut(s)	90, 1502
KpnI	GGTACC	2 cut(s)	1891, 2361
KroI	GCCGGC	1 cut(s)	1911
KroNI	GCCGGC	1 cut(s)	1913
Ksp22I	TGATCA	2 cut(s)	891, 1701
LguI	GCTCTTC	1 cut(s)	1383
LmnI	GCTCC	8 cut(s)	719, 1032, 1262, 1509, 1633, 1665, 1682, 1859
Lsp1109I	GCAGC	6 cut(s)	353, 764, 1198, 1360, 1424, 1914
LweI	GCATC	7 cut(s)	1615, 1785, 2041, 2196, 2475, 2476, 2527
MabI	ACCWGGT	1 cut(s)	1866
MaeI	CTAG	5 cut(s)	357, 683, 1622, 1742, 1856
MaeII	ACGT	2 cut(s)	15, 213
MaeIII	GTNAC	5 cut(s)	519, 847, 994, 1132, 1251
MfeI	CAATTG	1 cut(s)	582
MflI	RGATCY	2 cut(s)	115, 1615
MhlI	GDGCHC	9 cut(s)	99, 191, 255, 640, 1037, 1267, 1399, 1875, 2499
MlsI	TGGCCA	1 cut(s)	48
MluNI	TGGCCA	1 cut(s)	48
MlyI	GAGTC	1 cut(s)	527
MmeI	TCCRAC	2 cut(s)	634, 1852
Mox20I	TGGCCA	1 cut(s)	48
Mph1103I	ATGCAT	1 cut(s)	2542
MroNI	GCCGGC	1 cut(s)	1911
MroXI	GAANNNNTTC	1 cut(s)	2148
MscI	TGGCCA	1 cut(s)	48
MseI	TTAA	5 cut(s)	783, 1020, 1446, 2240, 2274
MslI	CAYNNNNRTG	2 cut(s)	1733, 2201
Msp20I	TGGCCA	1 cut(s)	48
MspA1I	CMGCKG	1 cut(s)	755
MspI	CCGG	4 cut(s)	24, 153, 1456, 1912
MspR9I	CCNGG	9 cut(s)	153, 1093, 1276, 1457, 1538, 1720, 1748, 1868, 2456
MunI	CAATTG	1 cut(s)	582
MvaI	CCWGG	7 cut(s)	1093, 1276, 1538, 1720, 1748, 1868, 2456
MvnI	CGCG	2 cut(s)	90, 2471
NaeI	GCCGGC	1 cut(s)	1913
NciI	CCSGG	2 cut(s)	153, 1457
NcoI	CCATGG	2 cut(s)	111, 1916
NgoMIV	GCCGGC	1 cut(s)	1911
NmuCI	GTSAC	3 cut(s)	519, 994, 1132
NsiI	ATGCAT	1 cut(s)	2542
NspI	RCATGY	2 cut(s)	2067, 2544
OliI	CACNNNNGTG	1 cut(s)	1733
PaeI	GCATGC	2 cut(s)	2067, 2544
PaeR7I	CTCGAG	1 cut(s)	98
PaqCI	CACCTGC	1 cut(s)	916
PciSI	GCTCTTC	1 cut(s)	1383
PcsI	WCGNNNNNNNCGW	3 cut(s)	81, 90, 1521
PdiI	GCCGGC	1 cut(s)	1913
PdmI	GAANNNNTTC	1 cut(s)	2148
PfeI	GAWTC	5 cut(s)	263, 290, 976, 1065, 1545
PflMI	CCANNNNNTGG	3 cut(s)	653, 755, 2170
PfoI	TCCNGGA	2 cut(s)	1091, 1718
PkrI	GCNGC	6 cut(s)	343, 754, 1213, 1375, 1414, 1929
Ple19I	CGATCG	1 cut(s)	88
PleI	GAGTC	1 cut(s)	526
PpsI	GAGTC	1 cut(s)	526
Psp124BI	GAGCTC	1 cut(s)	1037
Psp6I	CCWGG	7 cut(s)	1091, 1274, 1536, 1718, 1746, 1866, 2454
PspFI	CCCAGC	2 cut(s)	755, 1790
PspGI	CCWGG	7 cut(s)	1091, 1274, 1536, 1718, 1746, 1866, 2454
PspOMI	GGGCCC	1 cut(s)	251
PspPI	GGNCC	6 cut(s)	224, 251, 252, 614, 644, 2128
PstI	CTGCAG	1 cut(s)	343
PstNI	CAGNNNCTG	1 cut(s)	755
PsuI	RGATCY	2 cut(s)	115, 1615
PvuI	CGATCG	1 cut(s)	88
PvuII	CAGCTG	1 cut(s)	755
RsaI	GTAC	6 cut(s)	216, 553, 747, 1889, 1941, 2359
RsaNI	GTAC	6 cut(s)	215, 552, 746, 1888, 1940, 2358
RseI	CAYNNNNRTG	2 cut(s)	1733, 2201
SacI	GAGCTC	1 cut(s)	1037
SalI	GTCGAC	1 cut(s)	77
SapI	GCTCTTC	1 cut(s)	1383
SaqAI	TTAA	5 cut(s)	783, 1020, 1446, 2240, 2274
SatI	GCNGC	6 cut(s)	342, 753, 1212, 1374, 1413, 1928
Sau96I	GGNCC	6 cut(s)	224, 251, 252, 614, 644, 2128
SchI	GAGTC	1 cut(s)	527
ScrFI	CCNGG	9 cut(s)	153, 1093, 1276, 1457, 1538, 1720, 1748, 1868, 2456
SduI	GDGCHC	9 cut(s)	99, 191, 255, 640, 1037, 1267, 1399, 1875, 2499
SexAI	ACCWGGT	1 cut(s)	1866
SfaNI	GCATC	7 cut(s)	1615, 1785, 2041, 2196, 2475, 2476, 2527
SfcI	CTRYAG	2 cut(s)	339, 984
Sfr274I	CTCGAG	1 cut(s)	98
SgrDI	CGTCGACG	1 cut(s)	77
SinI	GGWCC	2 cut(s)	614, 644
SlaI	CTCGAG	1 cut(s)	98
SmiMI	CAYNNNNRTG	2 cut(s)	1733, 2201
SmlI	CTYRAG	4 cut(s)	98, 1003, 2186, 2498
SmoI	CTYRAG	4 cut(s)	98, 1003, 2186, 2498
SphI	GCATGC	2 cut(s)	2067, 2544
SsiI	CCGC	7 cut(s)	29, 481, 1586, 1840, 2209, 2471, 2489
SspMI	CTAG	5 cut(s)	357, 683, 1622, 1742, 1856
SstI	GAGCTC	1 cut(s)	1037
StyD4I	CCNGG	9 cut(s)	151, 1091, 1274, 1455, 1536, 1718, 1746, 1866, 2454
StyI	CCWWGG	3 cut(s)	111, 640, 1916
TaaI	ACNGT	4 cut(s)	348, 1976, 2027, 2036
TaiI	ACGT	2 cut(s)	18, 216
TaqI	TCGA	6 cut(s)	18, 78, 99, 700, 1543, 2527
TaqII	GACCGA	1 cut(s)	1149
TatI	WGTACW	1 cut(s)	551
TfiI	GAWTC	5 cut(s)	263, 290, 976, 1065, 1545
Tru1I	TTAA	5 cut(s)	783, 1020, 1446, 2240, 2274
Tru9I	TTAA	5 cut(s)	783, 1020, 1446, 2240, 2274
TscAI	CASTG	2 cut(s)	1212, 1667
TseFI	GTSAC	3 cut(s)	519, 994, 1132
TseI	GCWGC	6 cut(s)	341, 752, 1211, 1373, 1412, 1927
Tsp45I	GTSAC	3 cut(s)	519, 994, 1132
TspDTI	ATGAA	3 cut(s)	617, 1327, 1994
TspGWI	ACGGA	3 cut(s)	32, 1095, 2258
TspRI	CASTG	2 cut(s)	1212, 1667
Van91I	CCANNNNNTGG	3 cut(s)	653, 755, 2170
VneI	GTGCAC	1 cut(s)	1871
VpaK11BI	GGWCC	2 cut(s)	614, 644
XagI	CCTNNNNNAGG	1 cut(s)	2426
XapI	RAATTY	3 cut(s)	1096, 1652, 2247
XbaI	TCTAGA	1 cut(s)	1621
XceI	RCATGY	2 cut(s)	2067, 2544
XhoI	CTCGAG	1 cut(s)	98
XmiI	GTMKAC	2 cut(s)	78, 1846
XmnI	GAANNNNTTC	1 cut(s)	2148
XspI	CTAG	5 cut(s)	357, 683, 1622, 1742, 1856
ZraI	GACGTC	1 cut(s)	16
Zsp2I	ATGCAT	1 cut(s)	2542

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.