Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003023

Physical Location & Seq

Genomic Coordinates Reverse (-)

40945927 .. 40956010

Gene Structure 10084 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1275000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2961 bp

ATGGACAATGCCGACGTGGAAGCCGGTGCGGAAGAGGAGTTCCGTGGCCAGAGGGGTCGCAAGTATCGCCCCGTCGTCGACGACGATCGCGCCGTGCTCGAGATGTCGTCCATGGATCCTTCCTCCTCCTCCTCCTCCTCCTCTTCCTCGTCTTCTCTCCCCGTCCACCAAGCTTCACTCAAGAAAATAAAAGTGAGCACCCAAGAAAACATGGGTTCTAACGTACAAGAAGGGCCTTCTACTCATGTGCAAGCCAATGGGCCCCAAAAAGAATCAAAATTGGAACTATTTGGTTTTGATTCTCTCGTCAACATTCTTGGTCTGAAGAGTATGACAGATGAGCAGACTGCAGCACCATCAAGTCCTAGAGATGGTGAAGATATTGCTATCACTCAGGGGCGACCAAAGCCAAGTGATCTCAAATTGGGAACAATGATGGGTGTATTTGTGCCGTGCCTTCAAAACATATTGGGAATTATCTACTATATCCGCTTTTCTTGGATTGTTGGTATGGCTGGCATAGCTGAGTCACTATTATTGGTTTTCTTCTGTGGCTTGTGTACTTTCCTGACTTCAATATCATTGAGTGCAATCGCAACCAATGGTGCTATGAAGGGTGGTGGACCTTACTATCTAATTGGTCGTGCCCTTGGTCCAGAAGTTGGAGTTAGCATTGGATTATGTTTCTTTCTAGGAAATGCTGTTGCCGGATCTCTTTATGTATTGGGAGCTGTTGAAACCTTCTTGAAAGCTGTACCAGCAGCTGGGATTTTTAGAGAGACCACAAGAGTTAATGGAACATCAATTCCAATACAAACTCCGAGTTCACATGACCTACAAATCTATGGGATAGTTGTGACTATTCTTTTGTGCTTTATTGTGTTTGGTGGTGTGAAAATGATCAATCGGGTTGCACCTGCTTTCCTCATACCTGTTTTATTCTCGCTGTTCTGCATATATATCGGGATTGCTTTGGCGAGGAAGAATCACCCTGTAGATGGGGTCACGGGCTTGAGTTTGAACTCTTTTAAGGAAAACTGGAGCTCAGATTATCAGAGGACCAATAACAATGGAATCCCTGATCCTGATGGAAAAGTATCCTGGAATTTCAATGCAATGGTCGGCCTTTTTTTCCCTGCTGTGACAGGAATTATGGCAGGTTCAAATCGGTCAGCCTCACTGAAAGATACTCAGCGTTCAATTCCTATCGGAACACTGGCTGCAACTCTTACAACTACGGCAATGTATCTGGTCTCTGTGTTACTATTTGGAGCCCTTGCAACCAGGGAGAAGCTTTTGACTGACAGGCTACTTTCGGCTACAATAGCTTGGCCTTTCCCAGTATTCATTTACATTGGAATAATTCTTTCAACCTTAGGTGCTGCTCTTCAAAGCCTGACTGGTGCCCCCCGTCTCCTTGCAGCAATAGCCAATGATGACATTTTACCTGTTCTTAACTACTTCCGGGTTTCAGAGGGGAATGAGCCTCACATTGCTACCTTGTTTACAGCGCTCCTCTGTATCGGGTGTGTCGTTATTGGGAACCTGGATCTTATCACACCAACTATAACTATGTTTTTCCTTCTGTGTTATGCGGGTGTGAACTTATCTTGCTTCCTTCTGGATCTTCTAGATGCTCCCAGTTGGCGTCCTCGGTGGAAATTTCACCACTGGAGCCTCTCTCTTCTTGGAGCCTCACTTTGTATAGTGATCATGTTCTTGATCTCCTGGACATTCACTATTGTGTCTCTAGCCCTGGCAAGCCTTATTTATTATTATGTGAGCATCAAAGGCAAGGCTGGGGATTGGGGTGATGGTTTCAAGAGTGCATATTTCCAACTGGCTCTCCGCAGTCTACGATCTCTAGGAGCAAACCAGGTGCACCCAAAGAATTGGTACCCCATCCCATTGATATTCTGCCGGCCATGGGGGAAGCTGCCTGACAATGTACCCTGCCATCCCAAACTTGCTGACTTTGCCAACTGTATGAAGAAGAAGGGCAGGGGAATGTCCATCTTTTTCGCTATTATGGACGGTGATTACCGTGAATGTGCTGAAGATGCTAAGACTGCATGCAAACAGCTTGCTACCTACCTTGACTACAAGAATTGTGAAGGTGTAGCTGAGATTGTCGTGGCCCCCAGTGTGTGTGAAGGATTTCGGGGCATTGTCCAGACAATGGGTCTTGGAAACCTCAAGCCAAACATTGTGGTGATGCGGTATCCTGAGATATGGCGTCGTGAAAACTTAACGGAAATTCCAGCCACTTTCGTTGAAATAATTAACGATTGCATTGTTGCGAACAAGGCTGTTGTTATTGTCAAGGGTCTTGACGAGTGGCCTAATGAGTATCAGAGGCAGTATGGTACCATTGACTTGTATTGGATAGTGAGAGATGGGGGTCTCATGCTTCTTCTCTCTCAACTCCTCCTCACGAAGGAAAGCTTTGAGAGTTGCAAAATCCAGGTTTTCTGCATCGCGGAGGAGGATACTGATGCGGAGGGGCTCAAGGCTGATGTCAAGAAGTTTCTGTATGATCTTCGGATGCATGCTGAAGTGATTGTTGTAACAATGAAATCATGGGACGTGCAAGCAGACGGTGCTTCTCCTCAAGATGAATCCATGGAAGCGTACACTGGTGCTCAGCAGCGGATAGCTGATTACATGGCTAACATGAAGGCAACAGCTGAGAAACAAGGGACCCCTCTGATGGCTGACGGGAGGCAAGTTTTCGTGGATGAGCAGCAGGTGGAGAAGTTTCTTTACACTACGCTGAAGCTGAACTCGACAATACTCAGATACTCGAGAATGGCTGCGGTTGTGCTTGTGAGCTTACCTCCACCGCCGGCCAACCACCCGGCATATTTCTACATGGAGTACATGGATTTGTTGGTGGAGAATGTGCCGAGACTTCTAATTGTGAGAGGATACCGTAGAGACGTTGTAACTCTATTCACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0006820 GO:0006821 GO:0008150 GO:0008324 GO:0008509 GO:0008511 GO:0009674 GO:0009987 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015103 GO:0015108 GO:0015291 GO:0015293 GO:0015294 GO:0015296 GO:0015318 GO:0015370 GO:0015373 GO:0015377 GO:0015378 GO:0015379 GO:0015672 GO:0015698 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0034220 GO:0035725 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055064 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055081 GO:0055083 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097708 GO:0098655 GO:0098656 GO:0098660 GO:0098661 GO:0098662 GO:0098771 GO:0098791 GO:1902476

KEGG Pathways

Metabolic & Signaling

ko04966 map04966

Pfam Domains

Protein Families

AA_permease SLC12

Protein Analysis

987

Amino Acids

108.34

Weight (kDa)

6.2

Isoelectric Point (pI)

33.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease	PF00324	147 - 612	5.2e-55	Amino acid permease
AA_permease_2	PF13520	169 - 593	1.4e-15	Amino acid permease
SLC12	PF03522	654 - 775	2.3e-12	Solute carrier family 12
SLC12	PF03522	786 - 985	2.6e-26	Solute carrier family 12

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003023)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30450 AT1G30450 AT1G30450 AT1G30450
fragaria_vesca	FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910 FvH4_3g09910
malus_domestica	MD05G1275000.v1.1 MD10G1254500.v1.1
prunus_persica	Prupe.4G088900_v2.0.a1 Prupe.4G088900_v2.0.a1
pyrus_communis	pycom05g25640 pycom10g21010
rosa_chinensis	RchiOBHm_Chr5g0015611 RchiOBHm_Chr5g0015801 RchiOBHm_Chr5g0015811
rosa_laevigata	RLG00000032213 RLG00000032223
rosa_multiflora	Rmu_co8164906.1_g000001 Rmu_sc0003989.1_g000002 Rmu_sc0005803.1_g000020 Rmu_sc0007629.1_g000001 Rmu_sc0011313.1_g000001 Rmu_sc0011313.1_g000002 Rmu_sc0016973.1_g000001
rosa_roxburghii	Rroxscaffold_1G00060860
rosa_rugosa	Rorug05G0028900
rosa_samantha	Rh5AG122200 Rh5BG120800 Rh5CG131200 Rh5CG132900 Rh5CG133000 Rh5DG119800 Rh5DG120900
rosa_wichuraiana	Rw5G010540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	925, 2737
Acc36I	ACCTGC	3 cut(s)	925, 1148, 2737
Acc65I	GGTACC	2 cut(s)	1896, 2366
AccB1I	GGYRCC	3 cut(s)	1403, 1896, 2366
AccB7I	CCANNNNNTGG	3 cut(s)	662, 764, 2179
AccI	GTMKAC	2 cut(s)	78, 1855
AccII	CGCG	2 cut(s)	90, 2480
AclWI	GGATC	6 cut(s)	110, 123, 718, 1076, 1557, 1632
AcoI	YGGCCR	3 cut(s)	46, 1922, 2847
AcsI	RAATTY	3 cut(s)	1105, 1661, 2256
AcuI	CTGAAG	4 cut(s)	344, 2076, 2574, 2795
AcyI	GRCGYC	2 cut(s)	1648, 2236
AfaI	GTAC	8 cut(s)	225, 562, 756, 1898, 1950, 2368, 2633, 2879
AfeI	AGCGCT	1 cut(s)	1512
AfiI	CCNNNNNNNGG	8 cut(s)	371, 662, 764, 998, 1927, 2179, 2437, 2710
AhdI	GACNNNNNGTC	1 cut(s)	2181
AjiI	CACGTC	2 cut(s)	16, 2587
AjnI	CCWGG	7 cut(s)	1100, 1283, 1545, 1727, 1755, 1875, 2463
AleI	CACNNNNGTG	1 cut(s)	1742
AloI	GAACNNNNNNTCC	4 cut(s)	23, 55, 790, 822
Alw21I	GWGCWC	5 cut(s)	99, 200, 1046, 1884, 2644
Alw26I	GTCTC	7 cut(s)	773, 1258, 1418, 1752, 2408, 2902, 2931
Alw44I	GTGCAC	1 cut(s)	1880
AlwI	GGATC	6 cut(s)	110, 123, 718, 1076, 1557, 1632
AlwNI	CAGNNNCTG	1 cut(s)	764
Ama87I	CYCGRG	2 cut(s)	98, 2803
Aor51HI	AGCGCT	1 cut(s)	1512
AoxI	GGCC	9 cut(s)	46, 233, 260, 1123, 1331, 1922, 2136, 2339, 2847
ApaI	GGGCCC	1 cut(s)	264
ApaLI	GTGCAC	1 cut(s)	1880
ApeKI	GCWGC	9 cut(s)	350, 761, 1220, 1382, 1421, 1936, 2647, 2743, 2813
ApoI	RAATTY	3 cut(s)	1105, 1661, 2256
ArsI	GACNNNNNNTTYG	2 cut(s)	850, 882
Asp700I	GAANNNNTTC	1 cut(s)	2157
Asp718I	GGTACC	2 cut(s)	1896, 2366
AspLEI	GCGC	2 cut(s)	92, 1513
AspS9I	GGNCC	8 cut(s)	233, 260, 261, 623, 653, 1059, 2137, 2700
AsuC2I	CCSGG	2 cut(s)	1466, 2858
AsuHPI	GGTGA	6 cut(s)	386, 980, 1658, 1823, 2048, 2224
AvaI	CYCGRG	2 cut(s)	98, 2803
AvaII	GGWCC	4 cut(s)	623, 653, 1059, 2700
AxyI	CCTNAGG	1 cut(s)	1375
BaeGI	GKGCMC	4 cut(s)	264, 649, 1408, 1884
BalI	TGGCCA	1 cut(s)	48
BamHI	GGATCC	1 cut(s)	115
BanI	GGYRCC	3 cut(s)	1403, 1896, 2366
BanII	GRGCYC	4 cut(s)	264, 1046, 1276, 2508
BarI	GAAGNNNNNNTAC	2 cut(s)	2747, 2779
BbsI	GAAGAC	1 cut(s)	144
Bbv12I	GWGCWC	5 cut(s)	99, 200, 1046, 1884, 2644
BbvI	GCAGC	9 cut(s)	362, 773, 1207, 1369, 1433, 1923, 2659, 2755, 2800
BceAI	ACGGC	3 cut(s)	77, 436, 1254
BcgI	CGANNNNNNTGC	2 cut(s)	943, 977
BciT130I	CCWGG	7 cut(s)	1102, 1285, 1547, 1729, 1757, 1877, 2465
BciVI	GTATCC	4 cut(s)	1108, 2232, 2482, 2921
BclI	TGATCA	2 cut(s)	900, 1710
BcnI	CCSGG	2 cut(s)	1466, 2858
BcoDI	GTCTC	7 cut(s)	773, 1258, 1418, 1752, 2408, 2902, 2931
BfaI	CTAG	5 cut(s)	366, 692, 1631, 1751, 1865
BfmI	CTRYAG	2 cut(s)	348, 993
BfoI	RGCGCY	1 cut(s)	1514
BfuAI	ACCTGC	3 cut(s)	925, 1148, 2737
BfuI	GTATCC	4 cut(s)	1108, 2232, 2482, 2921
BisI	GCNGC	9 cut(s)	351, 762, 1221, 1383, 1422, 1937, 2648, 2744, 2814
BlpI	GCTNAGC	1 cut(s)	2643
BlsI	GCNGC	9 cut(s)	352, 763, 1222, 1384, 1423, 1938, 2649, 2745, 2815
Bme1390I	CCNGG	9 cut(s)	1102, 1285, 1466, 1547, 1729, 1757, 1877, 2465, 2858
Bme18I	GGWCC	4 cut(s)	623, 653, 1059, 2700
BmeRI	GACNNNNNGTC	1 cut(s)	2181
BmeT110I	CYCGRG	2 cut(s)	98, 2803
BmgBI	CACGTC	2 cut(s)	16, 2587
BmgT120I	GGNCC	8 cut(s)	233, 260, 261, 623, 653, 1059, 2137, 2700
BmrFI	CCNGG	9 cut(s)	1102, 1285, 1466, 1547, 1729, 1757, 1877, 2465, 2858
BmrI	ACTGGG	3 cut(s)	1334, 1635, 2136
BmsI	GCATC	7 cut(s)	1624, 1794, 2050, 2205, 2484, 2485, 2535
BmuI	ACTGGG	3 cut(s)	1334, 1635, 2136
BpiI	GAAGAC	1 cut(s)	144
BplI	GAGNNNNNCTC	4 cut(s)	1666, 1698, 2821, 2853
BpmI	CTGGAG	2 cut(s)	1060, 1693
Bpu1102I	GCTNAGC	1 cut(s)	2643
BpuEI	CTTGAG	5 cut(s)	164, 1033, 2180, 2492, 2595
BpuMI	CCSGG	2 cut(s)	1466, 2858
BsaHI	GRCGYC	2 cut(s)	1648, 2236
BsaI	GGTCTC	3 cut(s)	773, 1258, 2408
BsaJI	CCNNGG	8 cut(s)	43, 111, 649, 1284, 1652, 1755, 1925, 2622
BsaXI	ACNNNNNCTCC	2 cut(s)	1830, 1860
Bsc4I	CCNNNNNNNGG	8 cut(s)	371, 662, 764, 998, 1927, 2179, 2437, 2710
Bse118I	RCCGGY	3 cut(s)	23, 1920, 2845
Bse1I	ACTGG	9 cut(s)	1043, 1221, 1340, 1405, 1641, 1676, 1845, 2142, 2641
Bse21I	CCTNAGG	1 cut(s)	1375
Bse3DI	GCAATG	3 cut(s)	1122, 1248, 1491
BseBI	CCWGG	7 cut(s)	1102, 1285, 1547, 1729, 1757, 1877, 2465
BseDI	CCNNGG	8 cut(s)	43, 111, 649, 1284, 1652, 1755, 1925, 2622
BseGI	GGATG	4 cut(s)	1902, 1957, 2550, 2743
BseLI	CCNNNNNNNGG	8 cut(s)	371, 662, 764, 998, 1927, 2179, 2437, 2710
BseMI	GCAATG	3 cut(s)	1122, 1248, 1491
BseMII	CTCAG	9 cut(s)	407, 516, 1059, 1205, 2115, 2217, 2657, 2679, 2809
BseNI	ACTGG	9 cut(s)	1043, 1221, 1340, 1405, 1641, 1676, 1845, 2142, 2641
BseSI	GKGCMC	4 cut(s)	264, 649, 1408, 1884
BseXI	GCAGC	9 cut(s)	362, 773, 1207, 1369, 1433, 1923, 2659, 2755, 2800
BseYI	CCCAGC	2 cut(s)	764, 1799
Bsh1236I	CGCG	2 cut(s)	90, 2480
Bsh1285I	CGRYCG	1 cut(s)	88
BshFI	GGCC	9 cut(s)	48, 235, 262, 1125, 1333, 1924, 2138, 2341, 2849
BshNI	GGYRCC	3 cut(s)	1403, 1896, 2366
BsiEI	CGRYCG	1 cut(s)	88
BsiHKAI	GWGCWC	5 cut(s)	99, 200, 1046, 1884, 2644
BsiHKCI	CYCGRG	2 cut(s)	98, 2803
BsiSI	CCGG	6 cut(s)	24, 708, 1465, 1921, 2846, 2858
BslFI	GGGAC	2 cut(s)	2597, 2713
BslI	CCNNNNNNNGG	8 cut(s)	371, 662, 764, 998, 1927, 2179, 2437, 2710
BsmAI	GTCTC	7 cut(s)	773, 1258, 1418, 1752, 2408, 2902, 2931
BsmBI	CGTCTC	2 cut(s)	1418, 2931
BsmFI	GGGAC	2 cut(s)	2597, 2713
BsnI	GGCC	9 cut(s)	48, 235, 262, 1125, 1333, 1924, 2138, 2341, 2849
Bso31I	GGTCTC	3 cut(s)	773, 1258, 2408
BsoBI	CYCGRG	2 cut(s)	98, 2803
Bsp120I	GGGCCC	1 cut(s)	260
Bsp1720I	GCTNAGC	1 cut(s)	2643
Bsp19I	CCATGG	3 cut(s)	111, 1925, 2622
BspANI	GGCC	9 cut(s)	48, 235, 262, 1125, 1333, 1924, 2138, 2341, 2849
BspCNI	CTCAG	9 cut(s)	406, 517, 1058, 1204, 2116, 2218, 2656, 2680, 2808
BspFNI	CGCG	2 cut(s)	90, 2480
BspMAI	CTGCAG	1 cut(s)	352
BspMI	ACCTGC	3 cut(s)	925, 1148, 2737
BspPI	GGATC	6 cut(s)	110, 123, 718, 1076, 1557, 1632
BspQI	GCTCTTC	1 cut(s)	1392
BspT107I	GGYRCC	3 cut(s)	1403, 1896, 2366
BspTNI	GGTCTC	3 cut(s)	773, 1258, 2408
BsrDI	GCAATG	3 cut(s)	1122, 1248, 1491
BsrFI	RCCGGY	3 cut(s)	23, 1920, 2845
BsrI	ACTGG	9 cut(s)	1043, 1221, 1340, 1405, 1641, 1676, 1845, 2142, 2641
BssAI	RCCGGY	3 cut(s)	23, 1920, 2845
BssECI	CCNNGG	8 cut(s)	43, 111, 649, 1284, 1652, 1755, 1925, 2622
BssNI	GRCGYC	2 cut(s)	1648, 2236
BssT1I	CCWWGG	4 cut(s)	111, 649, 1925, 2622
Bst2UI	CCWGG	7 cut(s)	1102, 1285, 1547, 1729, 1757, 1877, 2465
Bst4CI	ACNGT	5 cut(s)	1985, 2036, 2045, 2600, 2933
Bst6I	CTCTTC	5 cut(s)	27, 148, 320, 1392, 1689
BstACI	GRCGYC	2 cut(s)	1648, 2236
BstAPI	GCANNNNNTGC	1 cut(s)	2600
BstC8I	GCNNGC	9 cut(s)	252, 517, 1762, 1922, 2074, 2085, 2550, 2592, 2847
BstDSI	CCRYGG	4 cut(s)	43, 111, 1925, 2622
BstENI	CCTNNNNNAGG	1 cut(s)	2435
BstF5I	GGATG	4 cut(s)	1902, 1957, 2550, 2743
BstFNI	CGCG	2 cut(s)	90, 2480
BstH2I	RGCGCY	1 cut(s)	1514
BstHHI	GCGC	2 cut(s)	92, 1513
BstMAI	GTCTC	7 cut(s)	773, 1258, 1418, 1752, 2408, 2902, 2931
BstMCI	CGRYCG	1 cut(s)	88
BstNI	CCWGG	7 cut(s)	1102, 1285, 1547, 1729, 1757, 1877, 2465
BstNSI	RCATGY	2 cut(s)	2076, 2552
BstSCI	CCNGG	9 cut(s)	1100, 1283, 1464, 1545, 1727, 1755, 1875, 2463, 2856
BstSFI	CTRYAG	2 cut(s)	348, 993
BstSLI	GKGCMC	4 cut(s)	264, 649, 1408, 1884
BstUI	CGCG	2 cut(s)	90, 2480
BstV1I	GCAGC	9 cut(s)	362, 773, 1207, 1369, 1433, 1923, 2659, 2755, 2800
BstV2I	GAAGAC	1 cut(s)	144
BstX2I	RGATCY	4 cut(s)	115, 710, 1549, 1624
BstXI	CCANNNNNNTGG	1 cut(s)	1893
BstYI	RGATCY	4 cut(s)	115, 710, 1549, 1624
Bsu36I	CCTNAGG	1 cut(s)	1375
BsuI	GTATCC	4 cut(s)	1108, 2232, 2482, 2921
BsuRI	GGCC	9 cut(s)	48, 235, 262, 1125, 1333, 1924, 2138, 2341, 2849
BtgI	CCRYGG	4 cut(s)	43, 111, 1925, 2622
BtgZI	GCGATG	1 cut(s)	2461
BtrI	CACGTC	2 cut(s)	16, 2587
BtsCI	GGATG	4 cut(s)	1902, 1957, 2550, 2743
BtsIMutI	CAGTG	5 cut(s)	1178, 1214, 1669, 2149, 2634
BveI	ACCTGC	3 cut(s)	925, 1148, 2737
Cac8I	GCNNGC	9 cut(s)	252, 517, 1762, 1922, 2074, 2085, 2550, 2592, 2847
CaiI	CAGNNNCTG	1 cut(s)	764
CfoI	GCGC	2 cut(s)	92, 1513
Cfr10I	RCCGGY	3 cut(s)	23, 1920, 2845
Cfr13I	GGNCC	8 cut(s)	233, 260, 261, 623, 653, 1059, 2137, 2700
CseI	GACGC	2 cut(s)	1637, 2225
CsiI	ACCWGGT	1 cut(s)	1875
Csp6I	GTAC	8 cut(s)	224, 561, 755, 1897, 1949, 2367, 2632, 2878
CspCI	CAANNNNNGTGG	4 cut(s)	2190, 2225, 2254, 2289
CviQI	GTAC	8 cut(s)	224, 561, 755, 1897, 1949, 2367, 2632, 2878
DriI	GACNNNNNGTC	1 cut(s)	2181
EaeI	YGGCCR	3 cut(s)	46, 1922, 2847
Eam1104I	CTCTTC	5 cut(s)	27, 148, 320, 1392, 1689
Eam1105I	GACNNNNNGTC	1 cut(s)	2181
EarI	CTCTTC	5 cut(s)	27, 148, 320, 1392, 1689
Ecl136II	GAGCTC	1 cut(s)	1044
Eco130I	CCWWGG	4 cut(s)	111, 649, 1925, 2622
Eco24I	GRGCYC	4 cut(s)	264, 1046, 1276, 2508
Eco31I	GGTCTC	3 cut(s)	773, 1258, 2408
Eco47I	GGWCC	4 cut(s)	623, 653, 1059, 2700
Eco47III	AGCGCT	1 cut(s)	1512
Eco53kI	GAGCTC	1 cut(s)	1044
Eco57I	CTGAAG	4 cut(s)	344, 2076, 2574, 2795
Eco81I	CCTNAGG	1 cut(s)	1375
Eco88I	CYCGRG	2 cut(s)	98, 2803
EcoICRI	GAGCTC	1 cut(s)	1044
EcoNI	CCTNNNNNAGG	1 cut(s)	2435
EcoO109I	RGGNCCY	3 cut(s)	233, 261, 2700
EcoRII	CCWGG	7 cut(s)	1100, 1283, 1545, 1727, 1755, 1875, 2463
EcoT14I	CCWWGG	4 cut(s)	111, 649, 1925, 2622
EcoT22I	ATGCAT	1 cut(s)	2550
EcoT38I	GRGCYC	4 cut(s)	264, 1046, 1276, 2508
ErhI	CCWWGG	4 cut(s)	111, 649, 1925, 2622
Esp3I	CGTCTC	2 cut(s)	1418, 2931
FalI	AAGNNNNNCTT	2 cut(s)	2429, 2461
FaqI	GGGAC	2 cut(s)	2597, 2713
FauI	CCCGC	1 cut(s)	1588
FbaI	TGATCA	2 cut(s)	900, 1710
FblI	GTMKAC	2 cut(s)	78, 1855
Fnu4HI	GCNGC	9 cut(s)	351, 762, 1221, 1383, 1422, 1937, 2648, 2744, 2814
FokI	GGATG	4 cut(s)	1889, 1944, 2557, 2750
FriOI	GRGCYC	4 cut(s)	264, 1046, 1276, 2508
Fsp4HI	GCNGC	9 cut(s)	351, 762, 1221, 1383, 1422, 1937, 2648, 2744, 2814
FspBI	CTAG	5 cut(s)	366, 692, 1631, 1751, 1865
GlaI	GCGC	2 cut(s)	91, 1512
GluI	GCNGC	9 cut(s)	351, 762, 1221, 1383, 1422, 1937, 2648, 2744, 2814
GsaI	CCCAGC	2 cut(s)	768, 1803
GsuI	CTGGAG	2 cut(s)	1060, 1693
HaeII	RGCGCY	1 cut(s)	1514
HaeIII	GGCC	9 cut(s)	48, 235, 262, 1125, 1333, 1924, 2138, 2341, 2849
HapII	CCGG	6 cut(s)	24, 708, 1465, 1921, 2846, 2858
HgaI	GACGC	2 cut(s)	1637, 2225
HhaI	GCGC	2 cut(s)	92, 1513
Hin1I	GRCGYC	2 cut(s)	1648, 2236
Hin6I	GCGC	2 cut(s)	90, 1511
HinP1I	GCGC	2 cut(s)	90, 1511
HincII	GTYRAC	2 cut(s)	79, 310
HindII	GTYRAC	2 cut(s)	79, 310
HindIII	AAGCTT	3 cut(s)	171, 1292, 2443
HinfI	GANTC	6 cut(s)	272, 299, 527, 985, 1074, 2618
HpaII	CCGG	6 cut(s)	24, 708, 1465, 1921, 2846, 2858
HphI	GGTGA	6 cut(s)	386, 980, 1658, 1823, 2048, 2224
Hpy99I	CGWCG	6 cut(s)	17, 77, 80, 83, 86, 2241
HpyCH4III	ACNGT	5 cut(s)	1985, 2036, 2045, 2600, 2933
HpyCH4IV	ACGT	4 cut(s)	15, 222, 2586, 2940
HpySE526I	ACGT	4 cut(s)	15, 222, 2586, 2940
Hsp92I	GRCGYC	2 cut(s)	1648, 2236
HspAI	GCGC	2 cut(s)	90, 1511
KflI	GGGWCCC	1 cut(s)	2700
KpnI	GGTACC	2 cut(s)	1900, 2370
KroI	GCCGGC	2 cut(s)	1920, 2845
KroNI	GCCGGC	2 cut(s)	1922, 2847
Ksp22I	TGATCA	2 cut(s)	900, 1710
LguI	GCTCTTC	1 cut(s)	1392
LmnI	GCTCC	8 cut(s)	728, 1041, 1271, 1518, 1642, 1674, 1691, 1868
Lsp1109I	GCAGC	9 cut(s)	362, 773, 1207, 1369, 1433, 1923, 2659, 2755, 2800
LweI	GCATC	7 cut(s)	1624, 1794, 2050, 2205, 2484, 2485, 2535
MabI	ACCWGGT	1 cut(s)	1875
MaeI	CTAG	5 cut(s)	366, 692, 1631, 1751, 1865
MaeII	ACGT	4 cut(s)	15, 222, 2586, 2940
MaeIII	GTNAC	7 cut(s)	528, 856, 1003, 1141, 1260, 2566, 2944
MflI	RGATCY	4 cut(s)	115, 710, 1549, 1624
MlsI	TGGCCA	1 cut(s)	48
MluNI	TGGCCA	1 cut(s)	48
MlyI	GAGTC	1 cut(s)	536
MmeI	TCCRAC	2 cut(s)	643, 1861
Mox20I	TGGCCA	1 cut(s)	48
Mph1103I	ATGCAT	1 cut(s)	2550
MroNI	GCCGGC	2 cut(s)	1920, 2845
MroXI	GAANNNNTTC	1 cut(s)	2157
MscI	TGGCCA	1 cut(s)	48
MseI	TTAA	5 cut(s)	792, 1029, 1455, 2249, 2283
MslI	CAYNNNNRTG	2 cut(s)	1742, 2210
Msp20I	TGGCCA	1 cut(s)	48
MspA1I	CMGCKG	3 cut(s)	764, 2650, 2687
MspI	CCGG	6 cut(s)	24, 708, 1465, 1921, 2846, 2858
MspR9I	CCNGG	9 cut(s)	1102, 1285, 1466, 1547, 1729, 1757, 1877, 2465, 2858
MvaI	CCWGG	7 cut(s)	1102, 1285, 1547, 1729, 1757, 1877, 2465
MvnI	CGCG	2 cut(s)	90, 2480
NaeI	GCCGGC	2 cut(s)	1922, 2847
NciI	CCSGG	2 cut(s)	1466, 2858
NcoI	CCATGG	3 cut(s)	111, 1925, 2622
NgoMIV	GCCGGC	2 cut(s)	1920, 2845
NmeAIII	GCCGAG	1 cut(s)	2931
NmuCI	GTSAC	4 cut(s)	528, 856, 1003, 1141
NsiI	ATGCAT	1 cut(s)	2550
NspI	RCATGY	2 cut(s)	2076, 2552
OliI	CACNNNNGTG	1 cut(s)	1742
PaeI	GCATGC	2 cut(s)	2076, 2552
PaeR7I	CTCGAG	2 cut(s)	98, 2803
PaqCI	CACCTGC	2 cut(s)	925, 2737
PciSI	GCTCTTC	1 cut(s)	1392
PcsI	WCGNNNNNNNCGW	3 cut(s)	81, 90, 1530
PdiI	GCCGGC	2 cut(s)	1922, 2847
PdmI	GAANNNNTTC	1 cut(s)	2157
PfeI	GAWTC	5 cut(s)	272, 299, 985, 1074, 2618
PflMI	CCANNNNNTGG	3 cut(s)	662, 764, 2179
PfoI	TCCNGGA	2 cut(s)	1100, 1727
PkrI	GCNGC	9 cut(s)	352, 763, 1222, 1384, 1423, 1938, 2649, 2745, 2815
Ple19I	CGATCG	1 cut(s)	88
PleI	GAGTC	1 cut(s)	535
PpsI	GAGTC	1 cut(s)	535
PpuMI	RGGWCCY	1 cut(s)	2700
Psp124BI	GAGCTC	1 cut(s)	1046
Psp5II	RGGWCCY	1 cut(s)	2700
Psp6I	CCWGG	7 cut(s)	1100, 1283, 1545, 1727, 1755, 1875, 2463
PspFI	CCCAGC	2 cut(s)	764, 1799
PspGI	CCWGG	7 cut(s)	1100, 1283, 1545, 1727, 1755, 1875, 2463
PspOMI	GGGCCC	1 cut(s)	260
PspPI	GGNCC	8 cut(s)	233, 260, 261, 623, 653, 1059, 2137, 2700
PspPPI	RGGWCCY	1 cut(s)	2700
PstI	CTGCAG	1 cut(s)	352
PstNI	CAGNNNCTG	1 cut(s)	764
PsuI	RGATCY	4 cut(s)	115, 710, 1549, 1624
PvuI	CGATCG	1 cut(s)	88
PvuII	CAGCTG	2 cut(s)	764, 2687
RsaI	GTAC	8 cut(s)	225, 562, 756, 1898, 1950, 2368, 2633, 2879
RsaNI	GTAC	8 cut(s)	224, 561, 755, 1897, 1949, 2367, 2632, 2878
RseI	CAYNNNNRTG	2 cut(s)	1742, 2210
SacI	GAGCTC	1 cut(s)	1046
SalI	GTCGAC	1 cut(s)	77
SapI	GCTCTTC	1 cut(s)	1392
SaqAI	TTAA	5 cut(s)	792, 1029, 1455, 2249, 2283
SatI	GCNGC	9 cut(s)	351, 762, 1221, 1383, 1422, 1937, 2648, 2744, 2814
Sau96I	GGNCC	8 cut(s)	233, 260, 261, 623, 653, 1059, 2137, 2700
SchI	GAGTC	1 cut(s)	536
ScrFI	CCNGG	9 cut(s)	1102, 1285, 1466, 1547, 1729, 1757, 1877, 2465, 2858
SexAI	ACCWGGT	1 cut(s)	1875
SfaNI	GCATC	7 cut(s)	1624, 1794, 2050, 2205, 2484, 2485, 2535
SfcI	CTRYAG	2 cut(s)	348, 993
Sfr274I	CTCGAG	2 cut(s)	98, 2803
SgrDI	CGTCGACG	1 cut(s)	77
SinI	GGWCC	4 cut(s)	623, 653, 1059, 2700
SlaI	CTCGAG	2 cut(s)	98, 2803
SmiMI	CAYNNNNRTG	2 cut(s)	1742, 2210
SmlI	CTYRAG	7 cut(s)	98, 179, 1012, 2195, 2507, 2610, 2803
SmoI	CTYRAG	7 cut(s)	98, 179, 1012, 2195, 2507, 2610, 2803
SphI	GCATGC	2 cut(s)	2076, 2552
SspMI	CTAG	5 cut(s)	366, 692, 1631, 1751, 1865
SstI	GAGCTC	1 cut(s)	1046
StyD4I	CCNGG	9 cut(s)	1100, 1283, 1464, 1545, 1727, 1755, 1875, 2463, 2856
StyI	CCWWGG	4 cut(s)	111, 649, 1925, 2622
TaaI	ACNGT	5 cut(s)	1985, 2036, 2045, 2600, 2933
TaiI	ACGT	4 cut(s)	18, 225, 2589, 2943
TaqI	TCGA	4 cut(s)	78, 99, 2786, 2804
TaqII	GACCGA	1 cut(s)	1158
TatI	WGTACW	2 cut(s)	560, 2877
TfiI	GAWTC	5 cut(s)	272, 299, 985, 1074, 2618
Tru1I	TTAA	5 cut(s)	792, 1029, 1455, 2249, 2283
Tru9I	TTAA	5 cut(s)	792, 1029, 1455, 2249, 2283
TscAI	CASTG	5 cut(s)	1185, 1221, 1676, 2149, 2641
TseFI	GTSAC	4 cut(s)	528, 856, 1003, 1141
TseI	GCWGC	9 cut(s)	350, 761, 1220, 1382, 1421, 1936, 2647, 2743, 2813
Tsp45I	GTSAC	4 cut(s)	528, 856, 1003, 1141
TspDTI	ATGAA	6 cut(s)	626, 1336, 2003, 2588, 2631, 2690
TspGWI	ACGGA	2 cut(s)	32, 2267
TspRI	CASTG	5 cut(s)	1185, 1221, 1676, 2149, 2641
Van91I	CCANNNNNTGG	3 cut(s)	662, 764, 2179
VneI	GTGCAC	1 cut(s)	1880
VpaK11BI	GGWCC	4 cut(s)	623, 653, 1059, 2700
XagI	CCTNNNNNAGG	1 cut(s)	2435
XapI	RAATTY	3 cut(s)	1105, 1661, 2256
XbaI	TCTAGA	1 cut(s)	1630
XceI	RCATGY	2 cut(s)	2076, 2552
XhoI	CTCGAG	2 cut(s)	98, 2803
XmiI	GTMKAC	2 cut(s)	78, 1855
XmnI	GAANNNNTTC	1 cut(s)	2157
XspI	CTAG	5 cut(s)	366, 692, 1631, 1751, 1865
Zsp2I	ATGCAT	1 cut(s)	2550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.