Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0002395

Physical Location & Seq

Genomic Coordinates Reverse (-)

7675252 .. 7677705

Gene Structure 2454 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g12840.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1326 bp

ATGGTGGTGATGATGATGCAGGGGAGGAGTAGTCCTCGTGTGTATCACGTGACAACGTATGGTGCTGATCCGACTGGGAACAGGGATAGCACTGAGGCTGTGGCTCAAGCATTTGCAGATGCTTTCCGTGGTCCATCAGAAGGTTTCTTGATGGAGGGAATCAGTAACCTTGGTGGTGCCCAAATCAATCTTGAAGGTGGCCAGTACCTAATTAGCAAACCCCTCCGGTTTCCGGCTGCCGGAGTAGGCAACATTATGATTCATGGAGGAACATTGAGAGCCTCAGATGACTTTCCGACTGATGGTTACTTGATTGATTTATCTGCTTCATCATCATCCCCCAACCAGAATGAAAAAACCAAAGAGAGCTCATCATCAATGTCTGCACAGCTTTCTTCATCATCCTACAACTATGAGTACATAACCCTAAAAGACCTCATGCTGGACGCTAACTACAGAGGTGGTGGTATTTCAGTCATAAACTCACTCAGAACCAGCATAGACAATTGTTACATAACCCATTTCAACACTGATGGTATTCTAGTCCAAAGTGGCCATGAAACCTACATCAGAAACTCCTTCCTAGGCCAGCACATCACCGCCGGCGGTGATCACAACGAAAGAAACTTTTCCGGTACTGCAATCAACCTAAAGGGAAATGACAATGCTGTTACAGATGTGGTGATTTTCTCTGCTGCTGTTGGTATAATGATTTCGGGTCCGGCTAATATACTTTCCGGTGTACATTGCTACAACAAGGCCACAGGTTTCGGAGGCGTTGGGATTTACTTGAGATTGCCTGGTTTGACACAGACTAGGATTGTCAATTCTTACATGGACTACACTGGTATAGTTGCTGAAGACCCAGTTCAGCTTGATATATCAAACAGCTTCTTCCTTGGTGATGCATACATTGTGTTGAAATCAATAAAGGGTGTAGCCAATGGGGTCAATATAGTTAACAACATGTTCAGTGGGTACAATAAAGGGATCGCAATTGTTCAACTGGACCAATCAAATGGGGGTTTCAAGGAGATAGAGCAAGTTGTTGTGGACAGGAACATTGTGAGGGGGATGAACATAAAGGGCACAATTGGAAGGAGAACTGTGCTAGGCAATGGAAGCTCATGGAATATAGACTTCAACCCAGTTCTTTTATTTCCTAATCTTATTAGGCATGTTCAGTATTCATTGAGCACTGGGGCTAGCAATGTGTTCCCCAACCATGCCCTAAGGAATGTTTCGGATAACCGGGTGGTGATCGAGTCGGATGTGGCGGTTCCGGCTTCGGTTTTTGTTACTGTGGATCAAGGAGTGTCAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0007049 GO:0007275 GO:0008150 GO:0009555 GO:0009556 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0016043 GO:0016787 GO:0016798 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0034293 GO:0043062 GO:0043934 GO:0044085 GO:0044703 GO:0045229 GO:0048229 GO:0048236 GO:0048646 GO:0048856 GO:0048869 GO:0051321 GO:0051704 GO:0071840 GO:0085029 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pectate_lyase_3

Protein Analysis

442

Amino Acids

47.24

Weight (kDa)

5.71

Isoelectric Point (pI)

34.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	17 - 257	1.7e-07	Rhamnogalacturonase A/epimerase, pectate lyase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002395)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050
fragaria_vesca	FvH4_3g12840 FvH4_6g36530
malus_domestica	MD05G1247500.v1.1 MD09G1167600.v1.1 MD10G1228300.v1.1
prunus_persica	Prupe.3G025600_v2.0.a1 Prupe.4G116600_v2.0.a1
pyrus_communis	pycom05g22350 pycom05g22360 pycom09g08510 pycom10g19190
rosa_chinensis	RchiOBHm_Chr2g0148991 RchiOBHm_Chr5g0020781
rosa_laevigata	RLG00000020361 RLG00000032562
rosa_multiflora	Rmu_co8368081.1_g000001 Rmu_co8378405.1_g000001 Rmu_sc0001397.1_g000010 Rmu_sc0002527.1_g000006 Rmu_sc0002527.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057520 Rroxscaffold_2G00098530
rosa_rugosa	Rorug02G0408600 Rorug05G0062800 Rorug05G0062900
rosa_samantha	Rh2AG467800 Rh2BG480400 Rh2CG454200 Rh2DG489300 Rh5AG152800 Rh5BG152500 Rh5CG164100 Rh5DG152000
rosa_wichuraiana	Rw2G038110 Rw5G013550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	176
AciI	CCGC	3 cut(s)	600, 606, 1277
AclWI	GGATC	3 cut(s)	62, 998, 1314
AcoI	YGGCCR	2 cut(s)	199, 553
AcuI	CTGAAG	1 cut(s)	879
AcvI	CACGTG	1 cut(s)	49
AfaI	GTAC	5 cut(s)	206, 419, 637, 744, 980
AfiI	CCNNNNNNNGG	5 cut(s)	140, 232, 239, 302, 442
AflIII	ACRYGT	1 cut(s)	966
AgsI	TTSAA	6 cut(s)	194, 526, 922, 1004, 1030, 1144
AjnI	CCWGG	1 cut(s)	799
AluBI	AGCT	6 cut(s)	369, 391, 874, 891, 1125, 1323
AluI	AGCT	6 cut(s)	369, 391, 874, 891, 1125, 1323
Alw21I	GWGCWC	2 cut(s)	371, 1199
AlwI	GGATC	3 cut(s)	62, 998, 1314
AoxI	GGCC	4 cut(s)	199, 553, 586, 759
ApeKI	GCWGC	2 cut(s)	236, 695
Asp700I	GAANNNNTTC	1 cut(s)	628
AspA2I	CCTAGG	1 cut(s)	583
AspS9I	GGNCC	3 cut(s)	131, 719, 1009
AsuC2I	CCSGG	1 cut(s)	1253
AsuHPI	GGTGA	6 cut(s)	19, 589, 620, 694, 914, 1270
AsuNHI	GCTAGC	1 cut(s)	1205
AvaII	GGWCC	3 cut(s)	131, 719, 1009
AvrII	CCTAGG	1 cut(s)	583
AxyI	CCTNAGG	1 cut(s)	1232
BaeGI	GKGCMC	2 cut(s)	181, 1091
BalI	TGGCCA	2 cut(s)	201, 555
BanI	GGYRCC	1 cut(s)	176
BanII	GRGCYC	1 cut(s)	371
BauI	CACGAG	1 cut(s)	36
BbrPI	CACGTG	1 cut(s)	49
BbsI	GAAGAC	1 cut(s)	867
Bbv12I	GWGCWC	2 cut(s)	371, 1199
BbvI	GCAGC	2 cut(s)	223, 682
BccI	CCATC	4 cut(s)	142, 145, 296, 527
BciT130I	CCWGG	1 cut(s)	801
BclI	TGATCA	1 cut(s)	610
BcnI	CCSGG	1 cut(s)	1253
BfaI	CTAG	5 cut(s)	542, 584, 816, 1112, 1206
BfmI	CTRYAG	1 cut(s)	454
BisI	GCNGC	2 cut(s)	237, 696
BlnI	CCTAGG	1 cut(s)	583
BlsI	GCNGC	2 cut(s)	238, 697
Bme1390I	CCNGG	2 cut(s)	801, 1253
Bme18I	GGWCC	3 cut(s)	131, 719, 1009
BmgT120I	GGNCC	3 cut(s)	131, 719, 1009
BmiI	GGNNCC	3 cut(s)	178, 720, 1281
BmrFI	CCNGG	2 cut(s)	801, 1253
BmrI	ACTGGG	4 cut(s)	84, 860, 1142, 1209
BmsI	GCATC	3 cut(s)	6, 109, 895
BmtI	GCTAGC	1 cut(s)	1209
BmuI	ACTGGG	4 cut(s)	84, 860, 1142, 1209
BpiI	GAAGAC	1 cut(s)	867
BplI	GAGNNNNNCTC	2 cut(s)	19, 51
BpuEI	CTTGAG	2 cut(s)	90, 811
BpuMI	CCSGG	1 cut(s)	1253
BsaAI	YACGTR	1 cut(s)	49
BsaJI	CCNNGG	4 cut(s)	127, 169, 583, 898
BsaWI	WCCGGW	3 cut(s)	225, 632, 737
BsaXI	ACNNNNNCTCC	2 cut(s)	16, 46
Bsc4I	CCNNNNNNNGG	5 cut(s)	140, 232, 239, 302, 442
Bse118I	RCCGGY	1 cut(s)	602
Bse1I	ACTGG	7 cut(s)	79, 202, 850, 866, 1011, 1148, 1204
Bse21I	CCTNAGG	1 cut(s)	1232
Bse3DI	GCAATG	3 cut(s)	745, 1123, 1216
BseBI	CCWGG	1 cut(s)	801
BseDI	CCNNGG	4 cut(s)	127, 169, 583, 898
BseGI	GGATG	4 cut(s)	335, 401, 1080, 1276
BseLI	CCNNNNNNNGG	5 cut(s)	140, 232, 239, 302, 442
BseMI	GCAATG	3 cut(s)	745, 1123, 1216
BseMII	CTCAG	3 cut(s)	84, 297, 502
BseNI	ACTGG	7 cut(s)	79, 202, 850, 866, 1011, 1148, 1204
BseRI	GAGGAG	1 cut(s)	40
BseSI	GKGCMC	2 cut(s)	181, 1091
BseXI	GCAGC	2 cut(s)	223, 682
BsgI	GTGCAG	1 cut(s)	369
BshFI	GGCC	4 cut(s)	201, 555, 588, 761
BshNI	GGYRCC	1 cut(s)	176
BsiHKAI	GWGCWC	2 cut(s)	371, 1199
BsiSI	CCGG	9 cut(s)	226, 233, 240, 603, 633, 722, 738, 1252, 1283
BslI	CCNNNNNNNGG	5 cut(s)	140, 232, 239, 302, 442
BsnI	GGCC	4 cut(s)	201, 555, 588, 761
Bsp1286I	GDGCHC	4 cut(s)	181, 371, 1091, 1199
Bsp1407I	TGTACA	1 cut(s)	742
Bsp143I	GATC	5 cut(s)	67, 610, 990, 1260, 1306
BspACI	CCGC	3 cut(s)	600, 606, 1277
BspANI	GGCC	4 cut(s)	201, 555, 588, 761
BspCNI	CTCAG	3 cut(s)	85, 296, 501
BspLI	GGNNCC	3 cut(s)	178, 720, 1281
BspOI	GCTAGC	1 cut(s)	1209
BspPI	GGATC	3 cut(s)	62, 998, 1314
BspT107I	GGYRCC	1 cut(s)	176
BsrDI	GCAATG	3 cut(s)	745, 1123, 1216
BsrFI	RCCGGY	1 cut(s)	602
BsrGI	TGTACA	1 cut(s)	742
BsrI	ACTGG	7 cut(s)	79, 202, 850, 866, 1011, 1148, 1204
BssAI	RCCGGY	1 cut(s)	602
BssECI	CCNNGG	4 cut(s)	127, 169, 583, 898
BssMI	GATC	5 cut(s)	67, 610, 990, 1260, 1306
BssSI	CACGAG	1 cut(s)	36
BssT1I	CCWWGG	3 cut(s)	169, 583, 898
Bst2BI	CACGAG	1 cut(s)	36
Bst2UI	CCWGG	1 cut(s)	801
Bst4CI	ACNGT	2 cut(s)	1108, 1303
BstAUI	TGTACA	1 cut(s)	742
BstBAI	YACGTR	1 cut(s)	49
BstC8I	GCNNGC	3 cut(s)	590, 604, 1207
BstDEI	CTNAG	4 cut(s)	93, 283, 488, 1232
BstDSI	CCRYGG	1 cut(s)	127
BstF5I	GGATG	4 cut(s)	335, 401, 1080, 1276
BstKTI	GATC	5 cut(s)	70, 613, 993, 1263, 1309
BstMBI	GATC	5 cut(s)	67, 610, 990, 1260, 1306
BstMWI	GCNNNNNNNGC	2 cut(s)	1122, 1283
BstNI	CCWGG	1 cut(s)	801
BstNSI	RCATGY	2 cut(s)	970, 1181
BstSCI	CCNGG	2 cut(s)	799, 1251
BstSFI	CTRYAG	1 cut(s)	454
BstSLI	GKGCMC	2 cut(s)	181, 1091
BstV1I	GCAGC	2 cut(s)	223, 682
BstV2I	GAAGAC	1 cut(s)	867
BstXI	CCANNNNNNTGG	1 cut(s)	1019
Bsu36I	CCTNAGG	1 cut(s)	1232
BsuRI	GGCC	4 cut(s)	201, 555, 588, 761
BtgI	CCRYGG	1 cut(s)	127
BtsCI	GGATG	4 cut(s)	335, 401, 1080, 1276
BtsIMutI	CAGTG	5 cut(s)	90, 528, 843, 979, 1197
Cac8I	GCNNGC	3 cut(s)	590, 604, 1207
Cfr10I	RCCGGY	1 cut(s)	602
Cfr13I	GGNCC	3 cut(s)	131, 719, 1009
CseI	GACGC	1 cut(s)	455
Csp6I	GTAC	5 cut(s)	205, 418, 636, 743, 979
CviAII	CATG	8 cut(s)	263, 439, 557, 835, 967, 1128, 1178, 1226
CviQI	GTAC	5 cut(s)	205, 418, 636, 743, 979
DdeI	CTNAG	4 cut(s)	93, 283, 488, 1232
DpnI	GATC	5 cut(s)	69, 612, 992, 1262, 1308
DpnII	GATC	5 cut(s)	67, 610, 990, 1260, 1306
EaeI	YGGCCR	2 cut(s)	199, 553
Ecl136II	GAGCTC	1 cut(s)	369
Eco130I	CCWWGG	3 cut(s)	169, 583, 898
Eco24I	GRGCYC	1 cut(s)	371
Eco47I	GGWCC	3 cut(s)	131, 719, 1009
Eco53kI	GAGCTC	1 cut(s)	369
Eco57I	CTGAAG	1 cut(s)	879
Eco72I	CACGTG	1 cut(s)	49
Eco81I	CCTNAGG	1 cut(s)	1232
EcoICRI	GAGCTC	1 cut(s)	369
EcoRII	CCWGG	1 cut(s)	799
EcoT14I	CCWWGG	3 cut(s)	169, 583, 898
EcoT22I	ATGCAT	1 cut(s)	910
EcoT38I	GRGCYC	1 cut(s)	371
ErhI	CCWWGG	3 cut(s)	169, 583, 898
FaeI	CATG	8 cut(s)	266, 442, 560, 838, 970, 1131, 1181, 1229
FatI	CATG	8 cut(s)	262, 438, 556, 834, 966, 1127, 1177, 1225
FbaI	TGATCA	1 cut(s)	610
Fnu4HI	GCNGC	2 cut(s)	237, 696
FokI	GGATG	4 cut(s)	322, 388, 1087, 1283
FriOI	GRGCYC	1 cut(s)	371
Fsp4HI	GCNGC	2 cut(s)	237, 696
FspBI	CTAG	5 cut(s)	542, 584, 816, 1112, 1206
GluI	GCNGC	2 cut(s)	237, 696
HaeIII	GGCC	4 cut(s)	201, 555, 588, 761
HapII	CCGG	9 cut(s)	226, 233, 240, 603, 633, 722, 738, 1252, 1283
HgaI	GACGC	1 cut(s)	455
Hin1II	CATG	8 cut(s)	266, 442, 560, 838, 970, 1131, 1181, 1229
HincII	GTYRAC	1 cut(s)	961
HindII	GTYRAC	1 cut(s)	961
HinfI	GANTC	3 cut(s)	159, 259, 1265
HpaI	GTTAAC	1 cut(s)	961
HpaII	CCGG	9 cut(s)	226, 233, 240, 603, 633, 722, 738, 1252, 1283
HphI	GGTGA	6 cut(s)	19, 589, 620, 694, 914, 1270
Hpy166II	GTNNAC	3 cut(s)	743, 961, 1054
Hpy188I	TCNGA	9 cut(s)	72, 139, 286, 297, 491, 572, 773, 1246, 1270
Hpy188III	TCNNGA	2 cut(s)	148, 191
Hpy8I	GTNNAC	3 cut(s)	743, 961, 1054
HpyAV	CCTTC	4 cut(s)	134, 188, 589, 1092
HpyCH4III	ACNGT	2 cut(s)	1108, 1303
HpyCH4IV	ACGT	2 cut(s)	48, 56
HpyCH4V	TGCA	5 cut(s)	19, 116, 386, 641, 908
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1122, 1283
HpyF3I	CTNAG	4 cut(s)	93, 283, 488, 1232
HpySE526I	ACGT	2 cut(s)	48, 56
Hsp92II	CATG	8 cut(s)	266, 442, 560, 838, 970, 1131, 1181, 1229
KroI	GCCGGC	1 cut(s)	602
KroNI	GCCGGC	1 cut(s)	604
Ksp22I	TGATCA	1 cut(s)	610
KspAI	GTTAAC	1 cut(s)	961
Kzo9I	GATC	5 cut(s)	67, 610, 990, 1260, 1306
Lsp1109I	GCAGC	2 cut(s)	223, 682
LweI	GCATC	3 cut(s)	6, 109, 895
MaeI	CTAG	5 cut(s)	542, 584, 816, 1112, 1206
MaeII	ACGT	2 cut(s)	48, 56
MaeIII	GTNAC	6 cut(s)	49, 164, 305, 509, 670, 1297
MalI	GATC	5 cut(s)	69, 612, 992, 1262, 1308
MboI	GATC	5 cut(s)	67, 610, 990, 1260, 1306
MboII	GAAGA	3 cut(s)	387, 872, 886
MfeI	CAATTG	3 cut(s)	505, 996, 1092
MhlI	GDGCHC	4 cut(s)	181, 371, 1091, 1199
MlsI	TGGCCA	2 cut(s)	201, 555
MluCI	AATT	5 cut(s)	210, 505, 826, 996, 1092
MluNI	TGGCCA	2 cut(s)	201, 555
MlyI	GAGTC	1 cut(s)	1274
MmeI	TCCRAC	3 cut(s)	95, 320, 1248
Mox20I	TGGCCA	2 cut(s)	201, 555
Mph1103I	ATGCAT	1 cut(s)	910
MreI	CGCCGGCG	1 cut(s)	602
MroNI	GCCGGC	1 cut(s)	602
MroXI	GAANNNNTTC	1 cut(s)	628
MscI	TGGCCA	2 cut(s)	201, 555
MseI	TTAA	1 cut(s)	960
Msp20I	TGGCCA	2 cut(s)	201, 555
MspI	CCGG	9 cut(s)	226, 233, 240, 603, 633, 722, 738, 1252, 1283
MspR9I	CCNGG	2 cut(s)	801, 1253
MunI	CAATTG	3 cut(s)	505, 996, 1092
MvaI	CCWGG	1 cut(s)	801
MwoI	GCNNNNNNNGC	2 cut(s)	1122, 1283
NaeI	GCCGGC	1 cut(s)	604
NciI	CCSGG	1 cut(s)	1253
NdeII	GATC	5 cut(s)	67, 610, 990, 1260, 1306
NgoMIV	GCCGGC	1 cut(s)	602
NheI	GCTAGC	1 cut(s)	1205
NlaIII	CATG	8 cut(s)	266, 442, 560, 838, 970, 1131, 1181, 1229
NlaIV	GGNNCC	3 cut(s)	178, 720, 1281
NmuCI	GTSAC	1 cut(s)	49
NsiI	ATGCAT	1 cut(s)	910
NspI	RCATGY	2 cut(s)	970, 1181
PciI	ACATGT	1 cut(s)	966
PdiI	GCCGGC	1 cut(s)	604
PdmI	GAANNNNTTC	1 cut(s)	628
PfeI	GAWTC	2 cut(s)	159, 259
PkrI	GCNGC	2 cut(s)	238, 697
PleI	GAGTC	1 cut(s)	1273
PmaCI	CACGTG	1 cut(s)	49
PmlI	CACGTG	1 cut(s)	49
PpsI	GAGTC	1 cut(s)	1273
Ppu21I	YACGTR	1 cut(s)	49
PscI	ACATGT	1 cut(s)	966
Psp124BI	GAGCTC	1 cut(s)	371
Psp6I	CCWGG	1 cut(s)	799
PspCI	CACGTG	1 cut(s)	49
PspGI	CCWGG	1 cut(s)	799
PspN4I	GGNNCC	3 cut(s)	178, 720, 1281
PspPI	GGNCC	3 cut(s)	131, 719, 1009
RsaI	GTAC	5 cut(s)	206, 419, 637, 744, 980
RsaNI	GTAC	5 cut(s)	205, 418, 636, 743, 979
SacI	GAGCTC	1 cut(s)	371
SaqAI	TTAA	1 cut(s)	960
SatI	GCNGC	2 cut(s)	237, 696
Sau3AI	GATC	5 cut(s)	67, 610, 990, 1260, 1306
Sau96I	GGNCC	3 cut(s)	131, 719, 1009
SchI	GAGTC	1 cut(s)	1274
ScrFI	CCNGG	2 cut(s)	801, 1253
SduI	GDGCHC	4 cut(s)	181, 371, 1091, 1199
SfaNI	GCATC	3 cut(s)	6, 109, 895
SfcI	CTRYAG	1 cut(s)	454
SgrAI	CRCCGGYG	1 cut(s)	602
SinI	GGWCC	3 cut(s)	131, 719, 1009
SmlI	CTYRAG	2 cut(s)	105, 790
SmoI	CTYRAG	2 cut(s)	105, 790
Sse9I	AATT	5 cut(s)	210, 505, 826, 996, 1092
SsiI	CCGC	3 cut(s)	600, 606, 1277
SspMI	CTAG	5 cut(s)	542, 584, 816, 1112, 1206
SstI	GAGCTC	1 cut(s)	371
StyD4I	CCNGG	2 cut(s)	799, 1251
StyI	CCWWGG	3 cut(s)	169, 583, 898
TaaI	ACNGT	2 cut(s)	1108, 1303
TaiI	ACGT	2 cut(s)	51, 59
TaqI	TCGA	1 cut(s)	1263
TasI	AATT	5 cut(s)	210, 505, 826, 996, 1092
TatI	WGTACW	2 cut(s)	417, 742
TfiI	GAWTC	2 cut(s)	159, 259
Tru1I	TTAA	1 cut(s)	960
Tru9I	TTAA	1 cut(s)	960
TscAI	CASTG	5 cut(s)	97, 535, 850, 979, 1204
TseFI	GTSAC	1 cut(s)	49
TseI	GCWGC	2 cut(s)	236, 695
Tsp45I	GTSAC	1 cut(s)	49
TspDTI	ATGAA	7 cut(s)	251, 318, 366, 387, 573, 1091, 1179
TspGWI	ACGGA	1 cut(s)	116
TspRI	CASTG	5 cut(s)	97, 535, 850, 979, 1204
VpaK11BI	GGWCC	3 cut(s)	131, 719, 1009
XceI	RCATGY	2 cut(s)	970, 1181
XmaJI	CCTAGG	1 cut(s)	583
XmnI	GAANNNNTTC	1 cut(s)	628
XspI	CTAG	5 cut(s)	542, 584, 816, 1112, 1206
Zsp2I	ATGCAT	1 cut(s)	910

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.