Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0002395

Physical Location & Seq

Genomic Coordinates Forward (+)

37906207 .. 37908526

Gene Structure 2320 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1247500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGGAGGCGACATTGCCGAAAACGGCTCTAATATGGTCAATCTTCATGGGATTTTGTATCTTCATCGTAATTCATGTCTACGCAGAAGATTCTCAAATGGGATATCGTCAGTTTTCCGGTGGCGATTACAACGATCATTTGCGAAAACTGCAAGAGTTCAAGGCTTCACTCCTCCGCCATGATTCGATTTCTGTTGCACCATCTTCAATCGTACCCTCTCCTAGTCCTAGCCTTATTGCTCCGCCGACGGGGAGCACTAGTCCACGTGTATATCACGTGACATCGTACGGCGCTGATCCGACGGGGAGCAGAGACAGCACTGATGCACTTGCTCAAGCAATGGCTGATGCATTTAGAGGCCCAACTGAAGGGTTCTTGATAGAGGGAATTGCCAATCTTGGAGGTGCCCAGATCGATCTTGAGGGTGGCCTTTATCTGATCAGCAGGCCCCTGAGGTTGCCTGGAGCTGGAGTAGGAAACCTTATGATTCATGGAGGAACGTTACGTGCCTCGGATGATTTTCCAGCTGAGGGTTACCTAATCGATTTATCGCCAACATCATCCGGTAACATGAAAGAAGAAACCAAAAAAGAGAGCTCAATGTCTGCACAGCTTTCATCATCATCCTACAACTATGAGTACATAACTCTCAAGGACCTATTGTTGGACTCTAACTACAGGGGAGGGGGCATTTCTGTCATAAACTCACTTAGGACCAGCATAGACAATTGTTACATAACACATTTCACCACCAATGGGATCCTAGTCCAAAGTGGCCACGAAACCTACATCCGAAACTCCTTCCTAGGGCAACACATCACCGCCGGTGGTGATCATGGCGAAAGGAACTTTTCGGGGACTGCGATTAACCTAATGGGGAATGACAATGCTGTGACAGATGTGGTAATTTTTTCTGCTGCCGTTGGCATTATGATTTCAGGTCCGGCCAACTTACTTTCCGGGGTCCATTGCTACAATAAGGCCACCGGATTTGGCGGCACGGGAATTTATTTAAGATTGCCCGGGTTGACACAAACACGAATCGTGAATTCGTACATGGACTACACCGGCATTGTTGCAGAAGATCCGGTGCAGCTTGATATCTCCAACAGTTTTTTCCTTGGTGATGCATACATTGTCCTAAAATCAATAAAAGGGGTTGCCAATGGGGTCAATATTGTAAACAACATGTTTAGTGGGTTCAATAAGGGGATAGGGATTGTTCAATTGGACCAAACAAATGGGCCTTTCAAAGATGTTGAACAAGTATATGTGGACAGGAACAATGTGAGGGGTATGAACATAAAGGGCACGGTTGGAAGGAGGTCTGTGCAAGGCAATGGGAGTTCATGGACCGTAGATTTCAATCCAATTCTACTATTTCCTAACCTTATTCGGCACGTTCAATACACGCTAAGGGCCGGTGGCAATGCGTTCCCGAACCATGCCCTCAGGAATATGTCGAATAACCGTGTTGTAATCGAGTCGAATGTGGCGGTTCCGGCTTCTGTTTTTGTGACAGTTGATCAAGGAGTGGCAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0007049 GO:0007275 GO:0008150 GO:0009555 GO:0009556 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0016043 GO:0016787 GO:0016798 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0034293 GO:0043062 GO:0043934 GO:0044085 GO:0044703 GO:0045229 GO:0048229 GO:0048236 GO:0048646 GO:0048856 GO:0048869 GO:0051321 GO:0051704 GO:0071840 GO:0085029 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pectate_lyase_3

Protein Analysis

515

Amino Acids

55.24

Weight (kDa)

5.94

Isoelectric Point (pI)

34.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	93 - 330	2.7e-08	Rhamnogalacturonase A/epimerase, pectate lyase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002395)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050
fragaria_vesca	FvH4_3g12840 FvH4_6g36530
malus_domestica	MD05G1247500.v1.1 MD09G1167600.v1.1 MD10G1228300.v1.1
prunus_persica	Prupe.3G025600_v2.0.a1 Prupe.4G116600_v2.0.a1
pyrus_communis	pycom05g22350 pycom05g22360 pycom09g08510 pycom10g19190
rosa_chinensis	RchiOBHm_Chr2g0148991 RchiOBHm_Chr5g0020781
rosa_laevigata	RLG00000020361 RLG00000032562
rosa_multiflora	Rmu_co8368081.1_g000001 Rmu_co8378405.1_g000001 Rmu_sc0001397.1_g000010 Rmu_sc0002527.1_g000006 Rmu_sc0002527.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057520 Rroxscaffold_2G00098530
rosa_rugosa	Rorug02G0408600 Rorug05G0062800 Rorug05G0062900
rosa_samantha	Rh2AG467800 Rh2BG480400 Rh2CG454200 Rh2DG489300 Rh5AG152800 Rh5BG152500 Rh5CG164100 Rh5DG152000
rosa_wichuraiana	Rw2G038110 Rw5G013550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	404
AccI	GTMKAC	1 cut(s)	78
AciI	CCGC	5 cut(s)	175, 242, 822, 996, 1496
AclI	AACGTT	1 cut(s)	500
AclWI	GGATC	4 cut(s)	290, 754, 767, 1079
AcoI	YGGCCR	2 cut(s)	775, 945
AcsI	RAATTY	2 cut(s)	1005, 1048
AcuI	CTGAAG	1 cut(s)	387
AcvI	CACGTG	2 cut(s)	266, 277
AfaI	GTAC	4 cut(s)	213, 287, 641, 1055
AfiI	CCNNNNNNNGG	7 cut(s)	248, 368, 467, 530, 664, 756, 807
AflIII	ACRYGT	2 cut(s)	265, 1188
AgsI	TTSAA	8 cut(s)	160, 207, 1204, 1226, 1252, 1262, 1366, 1406
AhlI	ACTAGT	1 cut(s)	257
AjnI	CCWGG	1 cut(s)	460
AjuI	GAANNNNNNNTTGG	2 cut(s)	1100, 1132
AluBI	AGCT	5 cut(s)	467, 527, 597, 613, 1096
AluI	AGCT	5 cut(s)	467, 527, 597, 613, 1096
Alw21I	GWGCWC	2 cut(s)	257, 599
Alw26I	GTCTC	1 cut(s)	306
AlwI	GGATC	4 cut(s)	290, 754, 767, 1079
Ama87I	CYCGRG	1 cut(s)	1022
AoxI	GGCC	8 cut(s)	358, 427, 446, 775, 945, 981, 1244, 1419
ApeKI	GCWGC	2 cut(s)	917, 1093
ApoI	RAATTY	2 cut(s)	1005, 1048
AspA2I	CCTAGG	1 cut(s)	805
AspLEI	GCGC	1 cut(s)	293
AsuC2I	CCSGG	3 cut(s)	961, 1023, 1024
AsuHPI	GGTGA	4 cut(s)	739, 811, 842, 1136
AvaI	CYCGRG	1 cut(s)	1022
AvaII	GGWCC	6 cut(s)	655, 714, 941, 964, 1231, 1353
AvrII	CCTAGG	1 cut(s)	805
AxyI	CCTNAGG	2 cut(s)	452, 1451
BaeGI	GKGCMC	2 cut(s)	409, 1313
BalI	TGGCCA	1 cut(s)	777
BamHI	GGATCC	1 cut(s)	759
BanI	GGYRCC	1 cut(s)	404
BanII	GRGCYC	1 cut(s)	599
BbrPI	CACGTG	2 cut(s)	266, 277
Bbv12I	GWGCWC	2 cut(s)	257, 599
BbvCI	CCTCAGC	1 cut(s)	528
BbvI	GCAGC	2 cut(s)	904, 1105
BccI	CCATC	1 cut(s)	208
BceAI	ACGGC	3 cut(s)	39, 304, 905
BciT130I	CCWGG	1 cut(s)	462
BclI	TGATCA	3 cut(s)	438, 832, 1525
BcnI	CCSGG	3 cut(s)	961, 1023, 1024
BcoDI	GTCTC	1 cut(s)	306
BcuI	ACTAGT	1 cut(s)	257
BfaI	CTAG	5 cut(s)	222, 228, 258, 764, 806
BfmI	CTRYAG	1 cut(s)	676
BfoI	RGCGCY	1 cut(s)	294
BisI	GCNGC	3 cut(s)	918, 997, 1094
BlnI	CCTAGG	1 cut(s)	805
BlsI	GCNGC	3 cut(s)	919, 998, 1095
Bme1390I	CCNGG	4 cut(s)	462, 961, 1023, 1024
Bme18I	GGWCC	6 cut(s)	655, 714, 941, 964, 1231, 1353
BmeT110I	CYCGRG	1 cut(s)	1022
BmiI	GGNNCC	5 cut(s)	406, 449, 761, 965, 1500
BmrFI	CCNGG	4 cut(s)	462, 961, 1023, 1024
BmsI	GCATC	3 cut(s)	313, 337, 1117
BpmI	CTGGAG	2 cut(s)	483, 489
Bpu10I	CCTNAGC	2 cut(s)	528, 1415
BpuEI	CTTGAG	3 cut(s)	318, 440, 635
BpuMI	CCSGG	3 cut(s)	961, 1023, 1024
Bsa29I	ATCGAT	2 cut(s)	414, 543
BsaAI	YACGTR	3 cut(s)	266, 277, 506
BsaBI	GATNNNNATC	1 cut(s)	1365
BsaJI	CCNNGG	5 cut(s)	510, 805, 960, 1022, 1120
BsaWI	WCCGGW	4 cut(s)	116, 563, 986, 1087
BsaXI	ACNNNNNCTCC	4 cut(s)	244, 274, 456, 486
Bsc4I	CCNNNNNNNGG	7 cut(s)	248, 368, 467, 530, 664, 756, 807
Bse118I	RCCGGY	3 cut(s)	824, 1067, 1421
Bse21I	CCTNAGG	2 cut(s)	452, 1451
Bse3DI	GCAATG	5 cut(s)	11, 345, 967, 1345, 1435
Bse8I	GATNNNNATC	1 cut(s)	1365
BseBI	CCWGG	1 cut(s)	462
BseCI	ATCGAT	2 cut(s)	414, 543
BseDI	CCNNGG	5 cut(s)	510, 805, 960, 1022, 1120
BseGI	GGATG	4 cut(s)	520, 560, 623, 789
BseJI	GATNNNNATC	1 cut(s)	1365
BseLI	CCNNNNNNNGG	7 cut(s)	248, 368, 467, 530, 664, 756, 807
BseMI	GCAATG	5 cut(s)	11, 345, 967, 1345, 1435
BseMII	CTCAG	3 cut(s)	443, 519, 1465
BseRI	GAGGAG	1 cut(s)	161
BseSI	GKGCMC	2 cut(s)	409, 1313
BseXI	GCAGC	2 cut(s)	904, 1105
BsgI	GTGCAG	2 cut(s)	591, 1112
BshFI	GGCC	8 cut(s)	360, 429, 448, 777, 947, 983, 1246, 1421
BshNI	GGYRCC	1 cut(s)	404
BshVI	ATCGAT	2 cut(s)	414, 543
BsiHKAI	GWGCWC	2 cut(s)	257, 599
BsiHKCI	CYCGRG	1 cut(s)	1022
BsiWI	CGTACG	1 cut(s)	285
BslFI	GGGAC	1 cut(s)	871
BslI	CCNNNNNNNGG	7 cut(s)	248, 368, 467, 530, 664, 756, 807
BsmAI	GTCTC	1 cut(s)	306
BsmFI	GGGAC	1 cut(s)	871
BsnI	GGCC	8 cut(s)	360, 429, 448, 777, 947, 983, 1246, 1421
BsoBI	CYCGRG	1 cut(s)	1022
Bsp1286I	GDGCHC	4 cut(s)	257, 409, 599, 1313
Bsp143I	GATC	9 cut(s)	133, 295, 411, 415, 438, 759, 832, 1084, 1525
BspACI	CCGC	5 cut(s)	175, 242, 822, 996, 1496
BspANI	GGCC	8 cut(s)	360, 429, 448, 777, 947, 983, 1246, 1421
BspCNI	CTCAG	3 cut(s)	444, 520, 1464
BspDI	ATCGAT	2 cut(s)	414, 543
BspLI	GGNNCC	5 cut(s)	406, 449, 761, 965, 1500
BspPI	GGATC	4 cut(s)	290, 754, 767, 1079
BspT107I	GGYRCC	1 cut(s)	404
BsrDI	GCAATG	5 cut(s)	11, 345, 967, 1345, 1435
BsrFI	RCCGGY	3 cut(s)	824, 1067, 1421
BssAI	RCCGGY	3 cut(s)	824, 1067, 1421
BssECI	CCNNGG	5 cut(s)	510, 805, 960, 1022, 1120
BssMI	GATC	9 cut(s)	133, 295, 411, 415, 438, 759, 832, 1084, 1525
BssT1I	CCWWGG	2 cut(s)	805, 1120
Bst2UI	CCWGG	1 cut(s)	462
Bst4CI	ACNGT	5 cut(s)	1112, 1315, 1357, 1472, 1522
BstBAI	YACGTR	3 cut(s)	266, 277, 506
BstC8I	GCNNGC	1 cut(s)	446
BstDEI	CTNAG	5 cut(s)	452, 528, 710, 1415, 1451
BstEII	GGTNACC	1 cut(s)	533
BstF5I	GGATG	4 cut(s)	520, 560, 623, 789
BstH2I	RGCGCY	1 cut(s)	294
BstHHI	GCGC	1 cut(s)	293
BstKTI	GATC	9 cut(s)	136, 298, 414, 418, 441, 762, 835, 1087, 1528
BstMAI	GTCTC	1 cut(s)	306
BstMBI	GATC	9 cut(s)	133, 295, 411, 415, 438, 759, 832, 1084, 1525
BstMWI	GCNNNNNNNGC	3 cut(s)	148, 315, 1502
BstNI	CCWGG	1 cut(s)	462
BstNSI	RCATGY	1 cut(s)	1192
BstPI	GGTNACC	1 cut(s)	533
BstSCI	CCNGG	4 cut(s)	460, 959, 1021, 1022
BstSFI	CTRYAG	1 cut(s)	676
BstSLI	GKGCMC	2 cut(s)	409, 1313
BstV1I	GCAGC	2 cut(s)	904, 1105
BstX2I	RGATCY	2 cut(s)	759, 1084
BstXI	CCANNNNNNTGG	1 cut(s)	1241
BstYI	RGATCY	2 cut(s)	759, 1084
Bsu15I	ATCGAT	2 cut(s)	414, 543
Bsu36I	CCTNAGG	2 cut(s)	452, 1451
BsuRI	GGCC	8 cut(s)	360, 429, 448, 777, 947, 983, 1246, 1421
BsuTUI	ATCGAT	2 cut(s)	414, 543
BtsCI	GGATG	4 cut(s)	520, 560, 623, 789
BtsIMutI	CAGTG	1 cut(s)	318
Cac8I	GCNNGC	1 cut(s)	446
CfoI	GCGC	1 cut(s)	293
Cfr10I	RCCGGY	3 cut(s)	824, 1067, 1421
Cfr9I	CCCGGG	1 cut(s)	1022
ClaI	ATCGAT	2 cut(s)	414, 543
Csp6I	GTAC	4 cut(s)	212, 286, 640, 1054
CspCI	CAANNNNNGTGG	2 cut(s)	973, 1008
CviQI	GTAC	4 cut(s)	212, 286, 640, 1054
DdeI	CTNAG	5 cut(s)	452, 528, 710, 1415, 1451
DpnI	GATC	9 cut(s)	135, 297, 413, 417, 440, 761, 834, 1086, 1527
DpnII	GATC	9 cut(s)	133, 295, 411, 415, 438, 759, 832, 1084, 1525
EaeI	YGGCCR	2 cut(s)	775, 945
EciI	GGCGGA	2 cut(s)	164, 231
Ecl136II	GAGCTC	1 cut(s)	597
Eco130I	CCWWGG	2 cut(s)	805, 1120
Eco24I	GRGCYC	1 cut(s)	599
Eco32I	GATATC	2 cut(s)	104, 1102
Eco47I	GGWCC	6 cut(s)	655, 714, 941, 964, 1231, 1353
Eco53kI	GAGCTC	1 cut(s)	597
Eco57I	CTGAAG	1 cut(s)	387
Eco72I	CACGTG	2 cut(s)	266, 277
Eco81I	CCTNAGG	2 cut(s)	452, 1451
Eco88I	CYCGRG	1 cut(s)	1022
Eco91I	GGTNACC	1 cut(s)	533
EcoICRI	GAGCTC	1 cut(s)	597
EcoO109I	RGGNCCY	2 cut(s)	447, 655
EcoO65I	GGTNACC	1 cut(s)	533
EcoRI	GAATTC	1 cut(s)	1048
EcoRII	CCWGG	1 cut(s)	460
EcoRV	GATATC	2 cut(s)	104, 1102
EcoT14I	CCWWGG	2 cut(s)	805, 1120
EcoT22I	ATGCAT	2 cut(s)	352, 1132
EcoT38I	GRGCYC	1 cut(s)	599
ErhI	CCWWGG	2 cut(s)	805, 1120
FaqI	GGGAC	1 cut(s)	871
FbaI	TGATCA	3 cut(s)	438, 832, 1525
FblI	GTMKAC	1 cut(s)	78
Fnu4HI	GCNGC	3 cut(s)	918, 997, 1094
FokI	GGATG	4 cut(s)	527, 547, 610, 776
FriOI	GRGCYC	1 cut(s)	599
Fsp4HI	GCNGC	3 cut(s)	918, 997, 1094
FspBI	CTAG	5 cut(s)	222, 228, 258, 764, 806
GlaI	GCGC	1 cut(s)	292
GluI	GCNGC	3 cut(s)	918, 997, 1094
GsuI	CTGGAG	2 cut(s)	483, 489
HaeII	RGCGCY	1 cut(s)	294
HaeIII	GGCC	8 cut(s)	360, 429, 448, 777, 947, 983, 1246, 1421
HhaI	GCGC	1 cut(s)	293
Hin6I	GCGC	1 cut(s)	291
HinP1I	GCGC	1 cut(s)	291
HincII	GTYRAC	1 cut(s)	1029
HindII	GTYRAC	1 cut(s)	1029
HinfI	GANTC	6 cut(s)	89, 182, 487, 668, 1041, 1484
HphI	GGTGA	4 cut(s)	739, 811, 842, 1136
Hpy166II	GTNNAC	5 cut(s)	79, 263, 1029, 1183, 1276
Hpy188I	TCNGA	4 cut(s)	300, 438, 514, 794
Hpy188III	TCNNGA	5 cut(s)	376, 419, 1045, 1438, 1453
Hpy8I	GTNNAC	5 cut(s)	79, 263, 1029, 1183, 1276
Hpy99I	CGWCG	2 cut(s)	250, 304
HpyAV	CCTTC	3 cut(s)	362, 811, 1314
HpyCH4III	ACNGT	5 cut(s)	1112, 1315, 1357, 1472, 1522
HpyCH4IV	ACGT	5 cut(s)	265, 276, 500, 505, 1401
HpyCH4V	TGCA	9 cut(s)	151, 197, 326, 350, 608, 1079, 1093, 1130, 1333
HpyF10VI	GCNNNNNNNGC	3 cut(s)	148, 315, 1502
HpyF3I	CTNAG	5 cut(s)	452, 528, 710, 1415, 1451
HpySE526I	ACGT	5 cut(s)	265, 276, 500, 505, 1401
HspAI	GCGC	1 cut(s)	291
Ksp22I	TGATCA	3 cut(s)	438, 832, 1525
Kzo9I	GATC	9 cut(s)	133, 295, 411, 415, 438, 759, 832, 1084, 1525
LmnI	GCTCC	4 cut(s)	244, 252, 306, 464
Lsp1109I	GCAGC	2 cut(s)	904, 1105
LweI	GCATC	3 cut(s)	313, 337, 1117
MaeI	CTAG	5 cut(s)	222, 228, 258, 764, 806
MaeII	ACGT	5 cut(s)	265, 276, 500, 505, 1401
MaeIII	GTNAC	7 cut(s)	277, 501, 533, 566, 731, 892, 1516
MalI	GATC	9 cut(s)	135, 297, 413, 417, 440, 761, 834, 1086, 1527
MboI	GATC	9 cut(s)	133, 295, 411, 415, 438, 759, 832, 1084, 1525
MboII	GAAGA	6 cut(s)	34, 52, 98, 195, 590, 1094
MfeI	CAATTG	2 cut(s)	727, 1226
MflI	RGATCY	2 cut(s)	759, 1084
MhlI	GDGCHC	4 cut(s)	257, 409, 599, 1313
MlsI	TGGCCA	1 cut(s)	777
MluCI	AATT	8 cut(s)	69, 387, 727, 906, 1005, 1048, 1226, 1371
MluNI	TGGCCA	1 cut(s)	777
MlyI	GAGTC	2 cut(s)	662, 1493
MmeI	TCCRAC	4 cut(s)	323, 645, 1131, 1297
Mox20I	TGGCCA	1 cut(s)	777
Mph1103I	ATGCAT	2 cut(s)	352, 1132
MscI	TGGCCA	1 cut(s)	777
MseI	TTAA	2 cut(s)	867, 1013
Msp20I	TGGCCA	1 cut(s)	777
MspA1I	CMGCKG	1 cut(s)	527
MspR9I	CCNGG	4 cut(s)	462, 961, 1023, 1024
MunI	CAATTG	2 cut(s)	727, 1226
MvaI	CCWGG	1 cut(s)	462
MwoI	GCNNNNNNNGC	3 cut(s)	148, 315, 1502
NciI	CCSGG	3 cut(s)	961, 1023, 1024
NdeII	GATC	9 cut(s)	133, 295, 411, 415, 438, 759, 832, 1084, 1525
NlaIV	GGNNCC	5 cut(s)	406, 449, 761, 965, 1500
NmuCI	GTSAC	3 cut(s)	277, 892, 1516
NsiI	ATGCAT	2 cut(s)	352, 1132
NspI	RCATGY	1 cut(s)	1192
PciI	ACATGT	1 cut(s)	1188
PcsI	WCGNNNNNNNCGW	1 cut(s)	860
PfeI	GAWTC	4 cut(s)	89, 182, 487, 1041
Pfl23II	CGTACG	1 cut(s)	285
PkrI	GCNGC	3 cut(s)	919, 998, 1095
PleI	GAGTC	2 cut(s)	662, 1492
PmaCI	CACGTG	2 cut(s)	266, 277
PmlI	CACGTG	2 cut(s)	266, 277
PpsI	GAGTC	2 cut(s)	662, 1492
Ppu21I	YACGTR	3 cut(s)	266, 277, 506
PpuMI	RGGWCCY	1 cut(s)	655
PscI	ACATGT	1 cut(s)	1188
Psp124BI	GAGCTC	1 cut(s)	599
Psp1406I	AACGTT	1 cut(s)	500
Psp5II	RGGWCCY	1 cut(s)	655
Psp6I	CCWGG	1 cut(s)	460
PspCI	CACGTG	2 cut(s)	266, 277
PspEI	GGTNACC	1 cut(s)	533
PspGI	CCWGG	1 cut(s)	460
PspLI	CGTACG	1 cut(s)	285
PspN4I	GGNNCC	5 cut(s)	406, 449, 761, 965, 1500
PspPPI	RGGWCCY	1 cut(s)	655
PsuI	RGATCY	2 cut(s)	759, 1084
PvuII	CAGCTG	1 cut(s)	527
RsaI	GTAC	4 cut(s)	213, 287, 641, 1055
RsaNI	GTAC	4 cut(s)	212, 286, 640, 1054
SacI	GAGCTC	1 cut(s)	599
SaqAI	TTAA	2 cut(s)	867, 1013
SatI	GCNGC	3 cut(s)	918, 997, 1094
Sau3AI	GATC	9 cut(s)	133, 295, 411, 415, 438, 759, 832, 1084, 1525
SchI	GAGTC	2 cut(s)	662, 1493
ScrFI	CCNGG	4 cut(s)	462, 961, 1023, 1024
SduI	GDGCHC	4 cut(s)	257, 409, 599, 1313
SfaNI	GCATC	3 cut(s)	313, 337, 1117
SfcI	CTRYAG	1 cut(s)	676
SgrAI	CRCCGGYG	1 cut(s)	824
SinI	GGWCC	6 cut(s)	655, 714, 941, 964, 1231, 1353
SmaI	CCCGGG	1 cut(s)	1024
SmlI	CTYRAG	3 cut(s)	333, 419, 650
SmoI	CTYRAG	3 cut(s)	333, 419, 650
SpeI	ACTAGT	1 cut(s)	257
Sse9I	AATT	8 cut(s)	69, 387, 727, 906, 1005, 1048, 1226, 1371
SsiI	CCGC	5 cut(s)	175, 242, 822, 996, 1496
SspI	AATATT	1 cut(s)	1177
SspMI	CTAG	5 cut(s)	222, 228, 258, 764, 806
SstI	GAGCTC	1 cut(s)	599
StyD4I	CCNGG	4 cut(s)	460, 959, 1021, 1022
StyI	CCWWGG	2 cut(s)	805, 1120
TaaI	ACNGT	5 cut(s)	1112, 1315, 1357, 1472, 1522
TaiI	ACGT	5 cut(s)	268, 279, 503, 508, 1404
TaqI	TCGA	6 cut(s)	185, 414, 543, 1463, 1482, 1487
TasI	AATT	8 cut(s)	69, 387, 727, 906, 1005, 1048, 1226, 1371
TatI	WGTACW	1 cut(s)	639
TauI	GCSGC	1 cut(s)	999
TfiI	GAWTC	4 cut(s)	89, 182, 487, 1041
Tru1I	TTAA	2 cut(s)	867, 1013
Tru9I	TTAA	2 cut(s)	867, 1013
TscAI	CASTG	1 cut(s)	325
TseFI	GTSAC	3 cut(s)	277, 892, 1516
TseI	GCWGC	2 cut(s)	917, 1093
Tsp45I	GTSAC	3 cut(s)	277, 892, 1516
TspDTI	ATGAA	8 cut(s)	34, 52, 62, 479, 587, 606, 1313, 1338
TspMI	CCCGGG	1 cut(s)	1022
TspRI	CASTG	1 cut(s)	325
VpaK11BI	GGWCC	6 cut(s)	655, 714, 941, 964, 1231, 1353
XapI	RAATTY	2 cut(s)	1005, 1048
XceI	RCATGY	1 cut(s)	1192
XmaI	CCCGGG	1 cut(s)	1022
XmaJI	CCTAGG	1 cut(s)	805
XmiI	GTMKAC	1 cut(s)	78
XspI	CTAG	5 cut(s)	222, 228, 258, 764, 806
Zsp2I	ATGCAT	2 cut(s)	352, 1132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.