Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0002395

Physical Location & Seq

Genomic Coordinates Forward (+)

32517513 .. 32519473

Gene Structure 1961 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1228300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGGAGGCAACAATGCCGAAAACGGCTCTAATATGGTCATTCTTCATGGCATTTTGTAGCTTCATCGTAATTCATGTCTACGCAGAGGACTCTCAAATGGGTTACCGTCAATTTTCCGGTGGCGATTACAATGATCATTTGCGAAAACTGCAAGAGTTCAAGGCTTCACTCACCCGCCACGATTCGCTTTCTGTTGCACCATCTTCAATCCCACCCTCTCCTAGTCCTAGCCTTATTGTTCCGCCAACGGGGAGCGCTAGTCCACGTGTTTATCGCGTGACATCGTACGGTGCTGATCCGACGGCGAGCAGAGACAGCAGTGATGCACTTGCTCAAGCAATGGCAGATGCATTTAGAGGCCCAACTGAAGGGTTCTTGATTGACGGTATTGCCAACCTTGGAGGTGCCCAGATCGATCTTGAGGGTGGCCATTATCTGATCAGCAGACCCCTGAGGTTGCCTGGAGCCGGAGTAGGAAATCTTATGATTCATGGAGGAACGTTACGCGCTTCGGACGATTTTCCAGCTGACGGATACCTGATTGATTTATCTCCAACATCATCAGCTAGCAAGAAAGAAGAAACCAAAAAAGGGAGCTCAATGTCCGCACAGCTTTCTTCATCATCCTACAACTATGAGTACATAACTCTCAAGGACCTACTTTTGAACTCTAACTACAGGGGAGGGGGCATTTCAGTCATAAACTCACTTAGGATAAGCATAGACAATTGTTACATAACACATTTCACCACCAATGGAATCCTAGTGCAAAGTGGCCACGAAACTTACATCCGAAACTCCTTCCTAGGGCAGCACATCACCGCTGGTAGTGATCGCGGTGAAAGAAACTTTTCCGGGACTGCGATTAACCTAATGGGGAATGATAATGCTGTGACAGATGTGGTGATTTTTTCTGCTGCGGTAGGTATAATGATTTCGGGTCCAGCCAACATACTTTCCGGGGTACATTGCTACAATAAGGCTACCGGATTTGGGGGCACCGGAATTTATTTGAGATTGCCCGGGTTGACACAAACCCGAATTGTGAATTCGTACATGGACTACAGCGGCATTGTTGCGGAAGATCCGGTGCAGCTTGATATCTCCAACAGTTTTTTCCTTGGTGAAGCATACATTGTCCTAAAATCAATAAAAGGGGTTGCCAATGGGGTCAATATTGTAAACAACATGTTTAGTGGGTTCGATAAGGGGGTAGAGATTGTTCAATTGGACCAAAATAATGGGCCTTTCAAAGAGGTTGAACAAGTATATGTGGACAGGAACAATGTGAGGGGCATGAACACAAAAGGCACAGTTGGAAGGAGGTCTGTGCAAGGCAATGGGAGCTCATGGACCGTAGATTTCAATCCAATTCTACTATTTCCTAACCTTATTCGGCATGTCCAATACACGCTGAGCGCCAGCGGCAATGCGTTCCCTAACCATACCTTAAGGAACGTGTCGAATAACCGTGTTGTGATCGAGTCGAATGTGGCGGCTCCGGCTTCTGTTTTTGTGACAGTTGATCAAGGAGTGGCAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0007049 GO:0007275 GO:0008150 GO:0009555 GO:0009556 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0016043 GO:0016787 GO:0016798 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0034293 GO:0043062 GO:0043934 GO:0044085 GO:0044703 GO:0045229 GO:0048229 GO:0048236 GO:0048646 GO:0048856 GO:0048869 GO:0051321 GO:0051704 GO:0071840 GO:0085029 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pectate_lyase_3

Protein Analysis

515

Amino Acids

55.25

Weight (kDa)

6.13

Isoelectric Point (pI)

32.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	93 - 330	4.4e-06	Rhamnogalacturonase A/epimerase, pectate lyase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002395)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050
fragaria_vesca	FvH4_3g12840 FvH4_6g36530
malus_domestica	MD05G1247500.v1.1 MD09G1167600.v1.1 MD10G1228300.v1.1
prunus_persica	Prupe.3G025600_v2.0.a1 Prupe.4G116600_v2.0.a1
pyrus_communis	pycom05g22350 pycom05g22360 pycom09g08510 pycom10g19190
rosa_chinensis	RchiOBHm_Chr2g0148991 RchiOBHm_Chr5g0020781
rosa_laevigata	RLG00000020361 RLG00000032562
rosa_multiflora	Rmu_co8368081.1_g000001 Rmu_co8378405.1_g000001 Rmu_sc0001397.1_g000010 Rmu_sc0002527.1_g000006 Rmu_sc0002527.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057520 Rroxscaffold_2G00098530
rosa_rugosa	Rorug02G0408600 Rorug05G0062800 Rorug05G0062900
rosa_samantha	Rh2AG467800 Rh2BG480400 Rh2CG454200 Rh2DG489300 Rh5AG152800 Rh5BG152500 Rh5CG164100 Rh5DG152000
rosa_wichuraiana	Rw2G038110 Rw5G013550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	404, 998
AccI	GTMKAC	1 cut(s)	78
AccII	CGCG	3 cut(s)	276, 507, 837
AclI	AACGTT	1 cut(s)	500
AclWI	GGATC	2 cut(s)	290, 1079
AcoI	YGGCCR	2 cut(s)	427, 775
AcsI	RAATTY	2 cut(s)	1005, 1048
AcuI	CTGAAG	1 cut(s)	387
AcvI	CACGTG	1 cut(s)	266
AfaI	GTAC	4 cut(s)	287, 641, 966, 1055
AfeI	AGCGCT	1 cut(s)	256
AfiI	CCNNNNNNNGG	7 cut(s)	248, 368, 467, 530, 591, 807, 993
AflII	CTTAAG	1 cut(s)	1450
AflIII	ACRYGT	3 cut(s)	265, 1188, 1458
AgsI	TTSAA	7 cut(s)	160, 207, 667, 1226, 1252, 1262, 1366
AjnI	CCWGG	1 cut(s)	460
AjuI	GAANNNNNNNTTGG	2 cut(s)	1100, 1132
AluBI	AGCT	7 cut(s)	60, 527, 566, 597, 613, 1096, 1347
AluI	AGCT	7 cut(s)	60, 527, 566, 597, 613, 1096, 1347
Alw21I	GWGCWC	2 cut(s)	599, 1349
Alw26I	GTCTC	1 cut(s)	306
AlwI	GGATC	2 cut(s)	290, 1079
Ama87I	CYCGRG	1 cut(s)	1022
Aor51HI	AGCGCT	1 cut(s)	256
AoxI	GGCC	4 cut(s)	358, 427, 775, 1244
ApeKI	GCWGC	3 cut(s)	811, 917, 1093
ApoI	RAATTY	2 cut(s)	1005, 1048
Asp700I	GAANNNNTTC	1 cut(s)	850
AspA2I	CCTAGG	1 cut(s)	805
AspLEI	GCGC	3 cut(s)	257, 509, 1421
AspS9I	GGNCC	6 cut(s)	359, 655, 941, 1231, 1244, 1353
AsuC2I	CCSGG	4 cut(s)	856, 961, 1023, 1024
AsuHPI	GGTGA	6 cut(s)	163, 739, 811, 851, 916, 1136
AsuNHI	GCTAGC	1 cut(s)	566
AvaI	CYCGRG	1 cut(s)	1022
AvaII	GGWCC	4 cut(s)	655, 941, 1231, 1353
AvrII	CCTAGG	1 cut(s)	805
AxyI	CCTNAGG	1 cut(s)	452
BaeGI	GKGCMC	2 cut(s)	409, 1001
BalI	TGGCCA	2 cut(s)	429, 777
BanI	GGYRCC	2 cut(s)	404, 998
BanII	GRGCYC	2 cut(s)	599, 1349
BbrPI	CACGTG	1 cut(s)	266
Bbv12I	GWGCWC	2 cut(s)	599, 1349
BbvI	GCAGC	3 cut(s)	823, 904, 1105
BccI	CCATC	1 cut(s)	208
BceAI	ACGGC	2 cut(s)	39, 318
BciT130I	CCWGG	1 cut(s)	462
BciVI	GTATCC	1 cut(s)	527
BclI	TGATCA	3 cut(s)	133, 438, 1525
BcnI	CCSGG	4 cut(s)	856, 961, 1023, 1024
BcoDI	GTCTC	1 cut(s)	306
BfaI	CTAG	6 cut(s)	222, 228, 258, 567, 764, 806
BfmI	CTRYAG	2 cut(s)	676, 1063
BfoI	RGCGCY	2 cut(s)	258, 1422
BfrI	CTTAAG	1 cut(s)	1450
BfuI	GTATCC	1 cut(s)	527
BisI	GCNGC	6 cut(s)	812, 918, 1069, 1094, 1426, 1497
BlnI	CCTAGG	1 cut(s)	805
BlpI	GCTNAGC	1 cut(s)	1415
BlsI	GCNGC	6 cut(s)	813, 919, 1070, 1095, 1427, 1498
Bme1390I	CCNGG	5 cut(s)	462, 856, 961, 1023, 1024
Bme18I	GGWCC	4 cut(s)	655, 941, 1231, 1353
BmeT110I	CYCGRG	1 cut(s)	1022
BmgT120I	GGNCC	6 cut(s)	359, 655, 941, 1231, 1244, 1353
BmiI	GGNNCC	5 cut(s)	406, 466, 942, 1000, 1500
BmrFI	CCNGG	5 cut(s)	462, 856, 961, 1023, 1024
BmsI	GCATC	2 cut(s)	313, 337
BmtI	GCTAGC	1 cut(s)	570
BpmI	CTGGAG	1 cut(s)	483
Bpu1102I	GCTNAGC	1 cut(s)	1415
BpuEI	CTTGAG	3 cut(s)	318, 440, 635
BpuMI	CCSGG	4 cut(s)	856, 961, 1023, 1024
Bsa29I	ATCGAT	1 cut(s)	414
BsaAI	YACGTR	1 cut(s)	266
BsaBI	GATNNNNATC	1 cut(s)	1365
BsaJI	CCNNGG	5 cut(s)	397, 805, 960, 1022, 1120
BsaWI	WCCGGW	4 cut(s)	116, 986, 1001, 1087
BsaXI	ACNNNNNCTCC	4 cut(s)	244, 274, 456, 486
Bsc4I	CCNNNNNNNGG	7 cut(s)	248, 368, 467, 530, 591, 807, 993
Bse21I	CCTNAGG	1 cut(s)	452
Bse3DI	GCAATG	4 cut(s)	345, 967, 1345, 1435
Bse8I	GATNNNNATC	1 cut(s)	1365
BseBI	CCWGG	1 cut(s)	462
BseCI	ATCGAT	1 cut(s)	414
BseDI	CCNNGG	5 cut(s)	397, 805, 960, 1022, 1120
BseGI	GGATG	2 cut(s)	623, 789
BseJI	GATNNNNATC	1 cut(s)	1365
BseLI	CCNNNNNNNGG	7 cut(s)	248, 368, 467, 530, 591, 807, 993
BseMI	GCAATG	4 cut(s)	345, 967, 1345, 1435
BseMII	CTCAG	2 cut(s)	443, 1406
BseSI	GKGCMC	2 cut(s)	409, 1001
BseXI	GCAGC	3 cut(s)	823, 904, 1105
BsgI	GTGCAG	1 cut(s)	1112
Bsh1236I	CGCG	3 cut(s)	276, 507, 837
BshFI	GGCC	4 cut(s)	360, 429, 777, 1246
BshNI	GGYRCC	2 cut(s)	404, 998
BshVI	ATCGAT	1 cut(s)	414
BsiHKAI	GWGCWC	2 cut(s)	599, 1349
BsiHKCI	CYCGRG	1 cut(s)	1022
BsiSI	CCGG	9 cut(s)	117, 468, 855, 960, 987, 1002, 1023, 1088, 1502
BsiWI	CGTACG	1 cut(s)	285
BslFI	GGGAC	1 cut(s)	871
BslI	CCNNNNNNNGG	7 cut(s)	248, 368, 467, 530, 591, 807, 993
BsmAI	GTCTC	1 cut(s)	306
BsmFI	GGGAC	1 cut(s)	871
BsnI	GGCC	4 cut(s)	360, 429, 777, 1246
BsoBI	CYCGRG	1 cut(s)	1022
Bsp1286I	GDGCHC	4 cut(s)	409, 599, 1001, 1349
Bsp143I	GATC	9 cut(s)	133, 295, 411, 415, 438, 832, 1084, 1479, 1525
Bsp1720I	GCTNAGC	1 cut(s)	1415
BspANI	GGCC	4 cut(s)	360, 429, 777, 1246
BspCNI	CTCAG	2 cut(s)	444, 1407
BspDI	ATCGAT	1 cut(s)	414
BspFNI	CGCG	3 cut(s)	276, 507, 837
BspLI	GGNNCC	5 cut(s)	406, 466, 942, 1000, 1500
BspOI	GCTAGC	1 cut(s)	570
BspPI	GGATC	2 cut(s)	290, 1079
BspT107I	GGYRCC	2 cut(s)	404, 998
BspTI	CTTAAG	1 cut(s)	1450
BsrDI	GCAATG	4 cut(s)	345, 967, 1345, 1435
BssECI	CCNNGG	5 cut(s)	397, 805, 960, 1022, 1120
BssMI	GATC	9 cut(s)	133, 295, 411, 415, 438, 832, 1084, 1479, 1525
BssT1I	CCWWGG	3 cut(s)	397, 805, 1120
Bst2UI	CCWGG	1 cut(s)	462
Bst4CI	ACNGT	8 cut(s)	107, 290, 386, 1112, 1315, 1357, 1472, 1522
BstAFI	CTTAAG	1 cut(s)	1450
BstBAI	YACGTR	1 cut(s)	266
BstC8I	GCNNGC	3 cut(s)	307, 568, 1423
BstDEI	CTNAG	3 cut(s)	452, 710, 1415
BstEII	GGTNACC	1 cut(s)	101
BstF5I	GGATG	2 cut(s)	623, 789
BstFNI	CGCG	3 cut(s)	276, 507, 837
BstH2I	RGCGCY	2 cut(s)	258, 1422
BstHHI	GCGC	3 cut(s)	257, 509, 1421
BstKTI	GATC	9 cut(s)	136, 298, 414, 418, 441, 835, 1087, 1482, 1528
BstMAI	GTCTC	1 cut(s)	306
BstMBI	GATC	9 cut(s)	133, 295, 411, 415, 438, 832, 1084, 1479, 1525
BstMWI	GCNNNNNNNGC	5 cut(s)	148, 315, 1344, 1425, 1502
BstNI	CCWGG	1 cut(s)	462
BstNSI	RCATGY	2 cut(s)	1192, 1403
BstPI	GGTNACC	1 cut(s)	101
BstSCI	CCNGG	5 cut(s)	460, 854, 959, 1021, 1022
BstSFI	CTRYAG	2 cut(s)	676, 1063
BstSLI	GKGCMC	2 cut(s)	409, 1001
BstUI	CGCG	3 cut(s)	276, 507, 837
BstV1I	GCAGC	3 cut(s)	823, 904, 1105
BstX2I	RGATCY	1 cut(s)	1084
BstXI	CCANNNNNNTGG	1 cut(s)	1241
BstYI	RGATCY	1 cut(s)	1084
Bsu15I	ATCGAT	1 cut(s)	414
Bsu36I	CCTNAGG	1 cut(s)	452
BsuI	GTATCC	1 cut(s)	527
BsuRI	GGCC	4 cut(s)	360, 429, 777, 1246
BsuTUI	ATCGAT	1 cut(s)	414
BtsCI	GGATG	2 cut(s)	623, 789
BtsI	GCAGTG	1 cut(s)	325
BtsIMutI	CAGTG	1 cut(s)	325
Cac8I	GCNNGC	3 cut(s)	307, 568, 1423
CfoI	GCGC	3 cut(s)	257, 509, 1421
Cfr13I	GGNCC	6 cut(s)	359, 655, 941, 1231, 1244, 1353
Cfr9I	CCCGGG	1 cut(s)	1022
ClaI	ATCGAT	1 cut(s)	414
Csp6I	GTAC	4 cut(s)	286, 640, 965, 1054
CviAII	CATG	8 cut(s)	46, 74, 491, 1057, 1189, 1297, 1350, 1400
CviQI	GTAC	4 cut(s)	286, 640, 965, 1054
DdeI	CTNAG	3 cut(s)	452, 710, 1415
DpnI	GATC	9 cut(s)	135, 297, 413, 417, 440, 834, 1086, 1481, 1527
DpnII	GATC	9 cut(s)	133, 295, 411, 415, 438, 832, 1084, 1479, 1525
EaeI	YGGCCR	2 cut(s)	427, 775
EciI	GGCGGA	1 cut(s)	231
Ecl136II	GAGCTC	2 cut(s)	597, 1347
Eco130I	CCWWGG	3 cut(s)	397, 805, 1120
Eco24I	GRGCYC	2 cut(s)	599, 1349
Eco32I	GATATC	1 cut(s)	1102
Eco47I	GGWCC	4 cut(s)	655, 941, 1231, 1353
Eco47III	AGCGCT	1 cut(s)	256
Eco53kI	GAGCTC	2 cut(s)	597, 1347
Eco57I	CTGAAG	1 cut(s)	387
Eco72I	CACGTG	1 cut(s)	266
Eco81I	CCTNAGG	1 cut(s)	452
Eco88I	CYCGRG	1 cut(s)	1022
Eco91I	GGTNACC	1 cut(s)	101
EcoICRI	GAGCTC	2 cut(s)	597, 1347
EcoO109I	RGGNCCY	1 cut(s)	655
EcoO65I	GGTNACC	1 cut(s)	101
EcoRI	GAATTC	1 cut(s)	1048
EcoRII	CCWGG	1 cut(s)	460
EcoRV	GATATC	1 cut(s)	1102
EcoT14I	CCWWGG	3 cut(s)	397, 805, 1120
EcoT22I	ATGCAT	1 cut(s)	352
EcoT38I	GRGCYC	2 cut(s)	599, 1349
ErhI	CCWWGG	3 cut(s)	397, 805, 1120
FaeI	CATG	8 cut(s)	49, 77, 494, 1060, 1192, 1300, 1353, 1403
FaqI	GGGAC	1 cut(s)	871
FatI	CATG	8 cut(s)	45, 73, 490, 1056, 1188, 1296, 1349, 1399
FauI	CCCGC	1 cut(s)	182
FbaI	TGATCA	3 cut(s)	133, 438, 1525
FblI	GTMKAC	1 cut(s)	78
Fnu4HI	GCNGC	6 cut(s)	812, 918, 1069, 1094, 1426, 1497
FokI	GGATG	2 cut(s)	610, 776
FriOI	GRGCYC	2 cut(s)	599, 1349
Fsp4HI	GCNGC	6 cut(s)	812, 918, 1069, 1094, 1426, 1497
FspBI	CTAG	6 cut(s)	222, 228, 258, 567, 764, 806
GlaI	GCGC	3 cut(s)	256, 508, 1420
GluI	GCNGC	6 cut(s)	812, 918, 1069, 1094, 1426, 1497
GsuI	CTGGAG	1 cut(s)	483
HaeII	RGCGCY	2 cut(s)	258, 1422
HaeIII	GGCC	4 cut(s)	360, 429, 777, 1246
HapII	CCGG	9 cut(s)	117, 468, 855, 960, 987, 1002, 1023, 1088, 1502
HhaI	GCGC	3 cut(s)	257, 509, 1421
Hin1II	CATG	8 cut(s)	49, 77, 494, 1060, 1192, 1300, 1353, 1403
Hin6I	GCGC	3 cut(s)	255, 507, 1419
HinP1I	GCGC	3 cut(s)	255, 507, 1419
HincII	GTYRAC	1 cut(s)	1029
HindII	GTYRAC	1 cut(s)	1029
HinfI	GANTC	5 cut(s)	89, 182, 487, 759, 1484
HpaII	CCGG	9 cut(s)	117, 468, 855, 960, 987, 1002, 1023, 1088, 1502
HphI	GGTGA	6 cut(s)	163, 739, 811, 851, 916, 1136
Hpy166II	GTNNAC	5 cut(s)	79, 263, 1029, 1183, 1276
Hpy188I	TCNGA	4 cut(s)	300, 438, 514, 794
Hpy188III	TCNNGA	2 cut(s)	376, 419
Hpy8I	GTNNAC	5 cut(s)	79, 263, 1029, 1183, 1276
Hpy99I	CGWCG	1 cut(s)	304
HpyAV	CCTTC	3 cut(s)	362, 811, 1314
HpyCH4III	ACNGT	8 cut(s)	107, 290, 386, 1112, 1315, 1357, 1472, 1522
HpyCH4IV	ACGT	3 cut(s)	265, 500, 1458
HpyCH4V	TGCA	7 cut(s)	151, 197, 326, 350, 769, 1093, 1333
HpyF10VI	GCNNNNNNNGC	5 cut(s)	148, 315, 1344, 1425, 1502
HpyF3I	CTNAG	3 cut(s)	452, 710, 1415
HpySE526I	ACGT	3 cut(s)	265, 500, 1458
Hsp92II	CATG	8 cut(s)	49, 77, 494, 1060, 1192, 1300, 1353, 1403
HspAI	GCGC	3 cut(s)	255, 507, 1419
Ksp22I	TGATCA	3 cut(s)	133, 438, 1525
Kzo9I	GATC	9 cut(s)	133, 295, 411, 415, 438, 832, 1084, 1479, 1525
LmnI	GCTCC	5 cut(s)	252, 464, 594, 1344, 1504
Lsp1109I	GCAGC	3 cut(s)	823, 904, 1105
LweI	GCATC	2 cut(s)	313, 337
MaeI	CTAG	6 cut(s)	222, 228, 258, 567, 764, 806
MaeII	ACGT	3 cut(s)	265, 500, 1458
MaeIII	GTNAC	6 cut(s)	101, 277, 501, 731, 892, 1516
MalI	GATC	9 cut(s)	135, 297, 413, 417, 440, 834, 1086, 1481, 1527
MboI	GATC	9 cut(s)	133, 295, 411, 415, 438, 832, 1084, 1479, 1525
MboII	GAAGA	5 cut(s)	34, 195, 590, 609, 1094
MfeI	CAATTG	2 cut(s)	727, 1226
MflI	RGATCY	1 cut(s)	1084
MhlI	GDGCHC	4 cut(s)	409, 599, 1001, 1349
MlsI	TGGCCA	2 cut(s)	429, 777
MluCI	AATT	8 cut(s)	69, 110, 727, 1005, 1041, 1048, 1226, 1371
MluNI	TGGCCA	2 cut(s)	429, 777
MlyI	GAGTC	2 cut(s)	83, 1493
MmeI	TCCRAC	4 cut(s)	323, 578, 1131, 1297
Mox20I	TGGCCA	2 cut(s)	429, 777
Mph1103I	ATGCAT	1 cut(s)	352
MroXI	GAANNNNTTC	1 cut(s)	850
MscI	TGGCCA	2 cut(s)	429, 777
MseI	TTAA	2 cut(s)	867, 1451
Msp20I	TGGCCA	2 cut(s)	429, 777
MspA1I	CMGCKG	4 cut(s)	527, 824, 1068, 1425
MspCI	CTTAAG	1 cut(s)	1450
MspI	CCGG	9 cut(s)	117, 468, 855, 960, 987, 1002, 1023, 1088, 1502
MspR9I	CCNGG	5 cut(s)	462, 856, 961, 1023, 1024
MunI	CAATTG	2 cut(s)	727, 1226
MvaI	CCWGG	1 cut(s)	462
MvnI	CGCG	3 cut(s)	276, 507, 837
MwoI	GCNNNNNNNGC	5 cut(s)	148, 315, 1344, 1425, 1502
NciI	CCSGG	4 cut(s)	856, 961, 1023, 1024
NdeII	GATC	9 cut(s)	133, 295, 411, 415, 438, 832, 1084, 1479, 1525
NheI	GCTAGC	1 cut(s)	566
NlaIII	CATG	8 cut(s)	49, 77, 494, 1060, 1192, 1300, 1353, 1403
NlaIV	GGNNCC	5 cut(s)	406, 466, 942, 1000, 1500
NmuCI	GTSAC	3 cut(s)	277, 892, 1516
NsiI	ATGCAT	1 cut(s)	352
NspI	RCATGY	2 cut(s)	1192, 1403
PciI	ACATGT	1 cut(s)	1188
PdmI	GAANNNNTTC	1 cut(s)	850
PfeI	GAWTC	3 cut(s)	182, 487, 759
Pfl23II	CGTACG	1 cut(s)	285
PfoI	TCCNGGA	1 cut(s)	854
PkrI	GCNGC	6 cut(s)	813, 919, 1070, 1095, 1427, 1498
PleI	GAGTC	2 cut(s)	83, 1492
PmaCI	CACGTG	1 cut(s)	266
PmlI	CACGTG	1 cut(s)	266
PpsI	GAGTC	2 cut(s)	83, 1492
Ppu21I	YACGTR	1 cut(s)	266
PpuMI	RGGWCCY	1 cut(s)	655
PscI	ACATGT	1 cut(s)	1188
Psp124BI	GAGCTC	2 cut(s)	599, 1349
Psp1406I	AACGTT	1 cut(s)	500
Psp5II	RGGWCCY	1 cut(s)	655
Psp6I	CCWGG	1 cut(s)	460
PspCI	CACGTG	1 cut(s)	266
PspEI	GGTNACC	1 cut(s)	101
PspGI	CCWGG	1 cut(s)	460
PspLI	CGTACG	1 cut(s)	285
PspN4I	GGNNCC	5 cut(s)	406, 466, 942, 1000, 1500
PspPI	GGNCC	6 cut(s)	359, 655, 941, 1231, 1244, 1353
PspPPI	RGGWCCY	1 cut(s)	655
PsrI	GAACNNNNNNTAC	4 cut(s)	659, 691, 1206, 1238
PsuI	RGATCY	1 cut(s)	1084
PvuII	CAGCTG	1 cut(s)	527
RsaI	GTAC	4 cut(s)	287, 641, 966, 1055
RsaNI	GTAC	4 cut(s)	286, 640, 965, 1054
SacI	GAGCTC	2 cut(s)	599, 1349
SaqAI	TTAA	2 cut(s)	867, 1451
SatI	GCNGC	6 cut(s)	812, 918, 1069, 1094, 1426, 1497
Sau3AI	GATC	9 cut(s)	133, 295, 411, 415, 438, 832, 1084, 1479, 1525
Sau96I	GGNCC	6 cut(s)	359, 655, 941, 1231, 1244, 1353
SchI	GAGTC	2 cut(s)	83, 1493
ScrFI	CCNGG	5 cut(s)	462, 856, 961, 1023, 1024
SduI	GDGCHC	4 cut(s)	409, 599, 1001, 1349
SfaNI	GCATC	2 cut(s)	313, 337
SfcI	CTRYAG	2 cut(s)	676, 1063
SinI	GGWCC	4 cut(s)	655, 941, 1231, 1353
SmaI	CCCGGG	1 cut(s)	1024
SmlI	CTYRAG	4 cut(s)	333, 419, 650, 1450
SmoI	CTYRAG	4 cut(s)	333, 419, 650, 1450
Sse9I	AATT	8 cut(s)	69, 110, 727, 1005, 1041, 1048, 1226, 1371
SspI	AATATT	1 cut(s)	1177
SspMI	CTAG	6 cut(s)	222, 228, 258, 567, 764, 806
SstI	GAGCTC	2 cut(s)	599, 1349
StyD4I	CCNGG	5 cut(s)	460, 854, 959, 1021, 1022
StyI	CCWWGG	3 cut(s)	397, 805, 1120
TaaI	ACNGT	8 cut(s)	107, 290, 386, 1112, 1315, 1357, 1472, 1522
TaiI	ACGT	3 cut(s)	268, 503, 1461
TaqI	TCGA	5 cut(s)	414, 1203, 1463, 1482, 1487
TasI	AATT	8 cut(s)	69, 110, 727, 1005, 1041, 1048, 1226, 1371
TatI	WGTACW	1 cut(s)	639
TauI	GCSGC	3 cut(s)	1071, 1428, 1499
TfiI	GAWTC	3 cut(s)	182, 487, 759
Tru1I	TTAA	2 cut(s)	867, 1451
Tru9I	TTAA	2 cut(s)	867, 1451
TscAI	CASTG	1 cut(s)	325
TseFI	GTSAC	3 cut(s)	277, 892, 1516
TseI	GCWGC	3 cut(s)	811, 917, 1093
Tsp45I	GTSAC	3 cut(s)	277, 892, 1516
TspDTI	ATGAA	6 cut(s)	34, 52, 62, 479, 609, 1313
TspGWI	ACGGA	1 cut(s)	546
TspMI	CCCGGG	1 cut(s)	1022
TspRI	CASTG	1 cut(s)	325
Vha464I	CTTAAG	1 cut(s)	1450
VpaK11BI	GGWCC	4 cut(s)	655, 941, 1231, 1353
XapI	RAATTY	2 cut(s)	1005, 1048
XceI	RCATGY	2 cut(s)	1192, 1403
XmaI	CCCGGG	1 cut(s)	1022
XmaJI	CCTAGG	1 cut(s)	805
XmiI	GTMKAC	1 cut(s)	78
XmnI	GAANNNNTTC	1 cut(s)	850
XspI	CTAG	6 cut(s)	222, 228, 258, 567, 764, 806
Zsp2I	ATGCAT	1 cut(s)	352

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.