RchiOBHm_Chr5g0020781

Polygalacturonase QRT3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002395
Physical Location & Seq
Reverse (-)
15029952 .. 15032329
2378 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30085

Sequence Viewer

Length: 1542 bp
ATGGAAGCTGCAATGCCGGGAAAGACTCTACTGTGGATTATGATTTGTAGCTTGTTTATCACAGTTCATGTTTACGGAGAATCCTCTCAGATGGGTCATTTTTCGAGGAGTGATTATCGAGACCAAATGCGAAGACTGCAAGAGTTCAAGGCCTCATTAACCGGGCACGATTCGGGTTCTTTTGTTGCACCATCTTCAGTCTCACCTGCTCCTAGCCCTAACCCTAACCCTCCGGGGAGGACTAGTCCTCGTGTGTATCACGTGACAACGTACGGTGCTGATCCTACCGGGAACAGGGACAGCACTGAGGCCTTGGCTCAGGCATTTGCAGATGCATTTAGAGGTCCATCAGAAGGGTTCTTGATGGAGGGAATTAGCAACCTTGGTGGTGCACAGATCAATCTTGAAGGTGGCCAGTACCTGATTAGCAAACCGCTCCGGCTTCCTGCTGCCGGAGTAGGAAACCTTATGATTCATGGAGGAACGTTGAGGGCCTCAGATAACTTCCCGACTGATGGGTACTTGATTGATTTATCTACTTCATCATCATCATCCCCCAACAAGAATGAAAAAAACAAAGAGAGCTCATCATCAATGTCTGCACAGCTTTCTTCATCATCCTACAACTATGAGTACATAACCCTAAAAGACCTTATGCTAGACGCCAACTACAGAGGTGGGGGCATTTCAGTCATAAACTCACTCAGAACCAGCATAGACAATTGCTACATAACCCATTTCAACACCGATGGCATTCTAGTCCAAAGTGGCCATGAAACCTACATCAGGAACTCCTTCCTAGGCCAGCACATCACCGCGGGCGGTGATCACAACGAAAGAAACTTTTCCGGCACTGCAATCAACCTTAAGGGAAATGACAATGCTGTGACAGATGTGGTGATTTTCTCCGCTGCTGTAGGTATAATGATTTCGGGACCGGCTAATATACTTTCGGGTGTACATTGCTACAATAAGGCCACAGGTTTCGGAGGTGTTGGGATTTACTTGAGATTGCCTGGTTTGACACAGACTAGGATTGTCAATTCTTACATGGACTACACTGGAATAGTTGCTGAAGACCCAGTTCAGCTTGATATATCAAACAGCTTCTTCCTTGGTGATGCATACATTGTGTTGAAATCAATAAAGGGTGTTGCCAATGGGGTTAATATAGTTAACAACATGTTCACTGGGTACAATAAGGGGATTGAAATTGTTCAATTGGACCAATCAAATGGGGGTTTCAAGGAGATTGAGCAAGTTGTTGTGGACAGGAATGCTGTGAGGGGGATGAACATAAAGGGCACAATTGGAAGGAGAACTGTGCAAGGCAATGGAACCTCATGGAATATAGACTTTAACCCAATTCTTCTATTTCCTAATCTTATTAGGCATGTGCAGTACTCTTTGAGCACTGGTGCTAGAAATGTGTTCCCCAACCATGCCCTGAGGAATGTATCAGATAATCGGGTCGTGATTGAGTCAGATGTGGCGGTTCCAGCTTCGGTTTTTGTGACAGTGGATCAAGGAATGTCAAACTAA

Protein Analysis

513

Amino Acids

55.28

Weight (kDa)

6.18

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 88 - 329 2.2e-08 Rhamnogalacturonase A/epimerase, pectate lyase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 214
Acc36I ACCTGC 1 cut(s) 214
AccBSI CCGCTC 1 cut(s) 436
AccII CGCG 1 cut(s) 818
AciI CCGC 6 cut(s) 434, 816, 818, 822, 909, 1493
AclI AACGTT 1 cut(s) 485
AclWI GGATC 2 cut(s) 275, 1530
AcoI YGGCCR 2 cut(s) 412, 769
AcuI CTGAAG 2 cut(s) 180, 1095
AcvI CACGTG 1 cut(s) 262
AcyI GRCGYC 1 cut(s) 663
AfaI GTAC 7 cut(s) 272, 419, 521, 635, 960, 1196, 1403
AfiI CCNNNNNNNGG 5 cut(s) 294, 353, 452, 515, 786
AflII CTTAAG 1 cut(s) 866
AflIII ACRYGT 1 cut(s) 1182
AgsI TTSAA 7 cut(s) 148, 407, 742, 1138, 1211, 1220, 1246
AhlI ACTAGT 1 cut(s) 242
AjnI CCWGG 1 cut(s) 1015
AluBI AGCT 7 cut(s) 8, 51, 585, 607, 1090, 1107, 1502
AluI AGCT 7 cut(s) 8, 51, 585, 607, 1090, 1107, 1502
Alw21I GWGCWC 3 cut(s) 394, 587, 1415
Alw26I GTCTC 2 cut(s) 114, 205
Alw44I GTGCAC 1 cut(s) 390
AlwI GGATC 2 cut(s) 275, 1530
AlwNI CAGNNNCTG 1 cut(s) 421
AoxI GGCC 7 cut(s) 150, 309, 412, 492, 769, 802, 975
ApaLI GTGCAC 1 cut(s) 390
ApeKI GCWGC 3 cut(s) 8, 449, 911
Asp700I GAANNNNTTC 3 cut(s) 794, 844, 1215
AspA2I CCTAGG 1 cut(s) 799
AspS9I GGNCC 4 cut(s) 344, 492, 935, 1225
AsuC2I CCSGG 4 cut(s) 18, 163, 234, 289
AsuHPI GGTGA 5 cut(s) 195, 805, 836, 910, 1130
AvaII GGWCC 3 cut(s) 344, 935, 1225
AvrII CCTAGG 1 cut(s) 799
AxyI CCTNAGG 1 cut(s) 1448
BaeGI GKGCMC 3 cut(s) 168, 394, 1307
BalI TGGCCA 2 cut(s) 414, 771
BanII GRGCYC 1 cut(s) 587
BauI CACGAG 1 cut(s) 249
BbrPI CACGTG 1 cut(s) 262
BbsI GAAGAC 2 cut(s) 139, 1083
Bbv12I GWGCWC 3 cut(s) 394, 587, 1415
BbvI GCAGC 2 cut(s) 436, 898
BccI CCATC 6 cut(s) 85, 199, 355, 358, 509, 743
BciT130I CCWGG 1 cut(s) 1017
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 4 cut(s) 18, 163, 234, 289
BcoDI GTCTC 2 cut(s) 114, 205
BcuI ACTAGT 1 cut(s) 242
BfaI CTAG 7 cut(s) 213, 243, 659, 758, 800, 1032, 1422
BfmI CTRYAG 2 cut(s) 670, 915
BfrI CTTAAG 1 cut(s) 866
BfuAI ACCTGC 1 cut(s) 214
BisI GCNGC 3 cut(s) 9, 450, 912
BlnI CCTAGG 1 cut(s) 799
BlsI GCNGC 3 cut(s) 10, 451, 913
BmcAI AGTACT 1 cut(s) 1403
Bme1390I CCNGG 5 cut(s) 18, 163, 234, 289, 1017
Bme18I GGWCC 3 cut(s) 344, 935, 1225
BmgT120I GGNCC 4 cut(s) 344, 492, 935, 1225
BmiI GGNNCC 3 cut(s) 936, 1339, 1497
BmrFI CCNGG 5 cut(s) 18, 163, 234, 289, 1017
BmrI ACTGGG 2 cut(s) 1076, 1200
BmsI GCATC 2 cut(s) 322, 1111
BmuI ACTGGG 2 cut(s) 1076, 1200
BpiI GAAGAC 2 cut(s) 139, 1083
Bpu10I CCTNAGC 1 cut(s) 318
BpuEI CTTGAG 1 cut(s) 1027
BpuMI CCSGG 4 cut(s) 18, 163, 234, 289
BsaAI YACGTR 1 cut(s) 262
BsaHI GRCGYC 1 cut(s) 663
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 6 cut(s) 233, 312, 382, 799, 816, 1114
BsaXI ACNNNNNCTCC 2 cut(s) 229, 259
Bsc4I CCNNNNNNNGG 5 cut(s) 294, 353, 452, 515, 786
Bse118I RCCGGY 1 cut(s) 937
Bse1I ACTGG 5 cut(s) 415, 1066, 1082, 1195, 1420
Bse21I CCTNAGG 1 cut(s) 1448
Bse3DI GCAATG 3 cut(s) 18, 961, 1339
BseBI CCWGG 1 cut(s) 1017
BseDI CCNNGG 6 cut(s) 233, 312, 382, 799, 816, 1114
BseGI GGATG 3 cut(s) 551, 617, 1296
BseLI CCNNNNNNNGG 5 cut(s) 294, 353, 452, 515, 786
BseMI GCAATG 3 cut(s) 18, 961, 1339
BseMII CTCAG 6 cut(s) 101, 297, 332, 510, 718, 1439
BseNI ACTGG 5 cut(s) 415, 1066, 1082, 1195, 1420
BseRI GAGGAG 1 cut(s) 121
BseSI GKGCMC 3 cut(s) 168, 394, 1307
BseXI GCAGC 2 cut(s) 436, 898
BsgI GTGCAG 2 cut(s) 585, 1418
Bsh1236I CGCG 1 cut(s) 818
BshFI GGCC 7 cut(s) 152, 311, 414, 494, 771, 804, 977
BsiHKAI GWGCWC 3 cut(s) 394, 587, 1415
BsiSI CCGG 8 cut(s) 17, 162, 233, 288, 439, 453, 849, 938
BsiWI CGTACG 1 cut(s) 270
BslFI GGGAC 2 cut(s) 311, 948
BslI CCNNNNNNNGG 5 cut(s) 294, 353, 452, 515, 786
BsmAI GTCTC 2 cut(s) 114, 205
BsmFI GGGAC 2 cut(s) 311, 948
BsmI GAATGC 2 cut(s) 753, 1282
BsnI GGCC 7 cut(s) 152, 311, 414, 494, 771, 804, 977
Bso31I GGTCTC 1 cut(s) 114
Bsp1286I GDGCHC 5 cut(s) 168, 394, 587, 1307, 1415
Bsp1407I TGTACA 1 cut(s) 958
Bsp143I GATC 4 cut(s) 280, 396, 826, 1522
BspACI CCGC 6 cut(s) 434, 816, 818, 822, 909, 1493
BspANI GGCC 7 cut(s) 152, 311, 414, 494, 771, 804, 977
BspCNI CTCAG 6 cut(s) 100, 298, 331, 509, 717, 1440
BspFNI CGCG 1 cut(s) 818
BspLI GGNNCC 3 cut(s) 936, 1339, 1497
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 2 cut(s) 275, 1530
BspTI CTTAAG 1 cut(s) 866
BspTNI GGTCTC 1 cut(s) 114
BsrBI CCGCTC 1 cut(s) 436
BsrDI GCAATG 3 cut(s) 18, 961, 1339
BsrFI RCCGGY 1 cut(s) 937
BsrGI TGTACA 1 cut(s) 958
BsrI ACTGG 5 cut(s) 415, 1066, 1082, 1195, 1420
BssAI RCCGGY 1 cut(s) 937
BssECI CCNNGG 6 cut(s) 233, 312, 382, 799, 816, 1114
BssMI GATC 4 cut(s) 280, 396, 826, 1522
BssNI GRCGYC 1 cut(s) 663
BssSI CACGAG 1 cut(s) 249
BssT1I CCWWGG 4 cut(s) 312, 382, 799, 1114
Bst2BI CACGAG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 1017
Bst4CI ACNGT 5 cut(s) 33, 64, 275, 1324, 1519
BstACI GRCGYC 1 cut(s) 663
BstAFI CTTAAG 1 cut(s) 866
BstAUI TGTACA 1 cut(s) 958
BstBAI YACGTR 1 cut(s) 262
BstC8I GCNNGC 2 cut(s) 806, 820
BstDEI CTNAG 6 cut(s) 87, 306, 318, 496, 704, 1448
BstDSI CCRYGG 1 cut(s) 816
BstENI CCTNNNNNAGG 1 cut(s) 784
BstF5I GGATG 3 cut(s) 551, 617, 1296
BstFNI CGCG 1 cut(s) 818
BstKTI GATC 4 cut(s) 283, 399, 829, 1525
BstMAI GTCTC 2 cut(s) 114, 205
BstMBI GATC 4 cut(s) 280, 396, 826, 1522
BstMWI GCNNNNNNNGC 2 cut(s) 136, 1499
BstNI CCWGG 1 cut(s) 1017
BstNSI RCATGY 2 cut(s) 1186, 1397
BstSCI CCNGG 5 cut(s) 16, 161, 232, 287, 1015
BstSFI CTRYAG 2 cut(s) 670, 915
BstSLI GKGCMC 3 cut(s) 168, 394, 1307
BstUI CGCG 1 cut(s) 818
BstV1I GCAGC 2 cut(s) 436, 898
BstV2I GAAGAC 2 cut(s) 139, 1083
BstXI CCANNNNNNTGG 1 cut(s) 1235
Bsu36I CCTNAGG 1 cut(s) 1448
BsuRI GGCC 7 cut(s) 152, 311, 414, 494, 771, 804, 977
BtgI CCRYGG 1 cut(s) 816
BtsCI GGATG 3 cut(s) 551, 617, 1296
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 6 cut(s) 303, 852, 1059, 1188, 1413, 1524
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 806, 820
CaiI CAGNNNCTG 1 cut(s) 421
Cfr10I RCCGGY 1 cut(s) 937
Cfr13I GGNCC 4 cut(s) 344, 492, 935, 1225
Cfr42I CCGCGG 1 cut(s) 819
CseI GACGC 1 cut(s) 671
Csp6I GTAC 7 cut(s) 271, 418, 520, 634, 959, 1195, 1402
CspCI CAANNNNNGTGG 2 cut(s) 367, 402
CviAII CATG 8 cut(s) 68, 476, 773, 1051, 1183, 1344, 1394, 1442
CviQI GTAC 7 cut(s) 271, 418, 520, 634, 959, 1195, 1402
DdeI CTNAG 6 cut(s) 87, 306, 318, 496, 704, 1448
DpnI GATC 4 cut(s) 282, 398, 828, 1524
DpnII GATC 4 cut(s) 280, 396, 826, 1522
EaeI YGGCCR 2 cut(s) 412, 769
Ecl136II GAGCTC 1 cut(s) 585
Eco130I CCWWGG 4 cut(s) 312, 382, 799, 1114
Eco147I AGGCCT 2 cut(s) 152, 311
Eco24I GRGCYC 1 cut(s) 587
Eco31I GGTCTC 1 cut(s) 114
Eco47I GGWCC 3 cut(s) 344, 935, 1225
Eco53kI GAGCTC 1 cut(s) 585
Eco57I CTGAAG 2 cut(s) 180, 1095
Eco72I CACGTG 1 cut(s) 262
Eco81I CCTNAGG 1 cut(s) 1448
EcoICRI GAGCTC 1 cut(s) 585
EcoNI CCTNNNNNAGG 1 cut(s) 784
EcoO109I RGGNCCY 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 1015
EcoT14I CCWWGG 4 cut(s) 312, 382, 799, 1114
EcoT22I ATGCAT 2 cut(s) 337, 1126
EcoT38I GRGCYC 1 cut(s) 587
ErhI CCWWGG 4 cut(s) 312, 382, 799, 1114
FaeI CATG 8 cut(s) 71, 479, 776, 1054, 1186, 1347, 1397, 1445
FaqI GGGAC 2 cut(s) 311, 948
FatI CATG 8 cut(s) 67, 475, 772, 1050, 1182, 1343, 1393, 1441
FauI CCCGC 1 cut(s) 811
FbaI TGATCA 1 cut(s) 826
Fnu4HI GCNGC 3 cut(s) 9, 450, 912
FokI GGATG 3 cut(s) 538, 604, 1303
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 3 cut(s) 9, 450, 912
FspBI CTAG 7 cut(s) 213, 243, 659, 758, 800, 1032, 1422
GluI GCNGC 3 cut(s) 9, 450, 912
HaeIII GGCC 7 cut(s) 152, 311, 414, 494, 771, 804, 977
HapII CCGG 8 cut(s) 17, 162, 233, 288, 439, 453, 849, 938
HgaI GACGC 1 cut(s) 671
Hin1I GRCGYC 1 cut(s) 663
Hin1II CATG 8 cut(s) 71, 479, 776, 1054, 1186, 1347, 1397, 1445
HincII GTYRAC 1 cut(s) 1177
HindII GTYRAC 1 cut(s) 1177
HinfI GANTC 5 cut(s) 25, 80, 170, 472, 1481
HpaI GTTAAC 1 cut(s) 1177
HpaII CCGG 8 cut(s) 17, 162, 233, 288, 439, 453, 849, 938
HphI GGTGA 5 cut(s) 195, 805, 836, 910, 1130
Hpy166II GTNNAC 6 cut(s) 73, 392, 959, 1177, 1188, 1270
Hpy188I TCNGA 7 cut(s) 90, 352, 499, 707, 989, 1462, 1486
Hpy188III TCNNGA 7 cut(s) 119, 361, 404, 508, 787, 933, 1474
Hpy8I GTNNAC 6 cut(s) 73, 392, 959, 1177, 1188, 1270
HpyAV CCTTC 4 cut(s) 347, 401, 805, 1308
HpyCH4III ACNGT 5 cut(s) 33, 64, 275, 1324, 1519
HpyCH4IV ACGT 3 cut(s) 261, 269, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 136, 1499
HpyF3I CTNAG 6 cut(s) 87, 306, 318, 496, 704, 1448
HpySE526I ACGT 3 cut(s) 261, 269, 485
Hsp92I GRCGYC 1 cut(s) 663
Hsp92II CATG 8 cut(s) 71, 479, 776, 1054, 1186, 1347, 1397, 1445
Ksp22I TGATCA 1 cut(s) 826
KspAI GTTAAC 1 cut(s) 1177
KspI CCGCGG 1 cut(s) 819
Kzo9I GATC 4 cut(s) 280, 396, 826, 1522
LmnI GCTCC 2 cut(s) 214, 441
Lsp1109I GCAGC 2 cut(s) 436, 898
LweI GCATC 2 cut(s) 322, 1111
MaeI CTAG 7 cut(s) 213, 243, 659, 758, 800, 1032, 1422
MaeII ACGT 3 cut(s) 261, 269, 485
MaeIII GTNAC 3 cut(s) 262, 886, 1513
MalI GATC 4 cut(s) 282, 398, 828, 1524
MbiI CCGCTC 1 cut(s) 436
MboI GATC 4 cut(s) 280, 396, 826, 1522
MboII GAAGA 6 cut(s) 144, 186, 603, 1088, 1102, 1361
MfeI CAATTG 3 cut(s) 721, 1220, 1308
MhlI GDGCHC 5 cut(s) 168, 394, 587, 1307, 1415
MlsI TGGCCA 2 cut(s) 414, 771
MluCI AATT 7 cut(s) 372, 721, 1042, 1212, 1220, 1308, 1365
MluNI TGGCCA 2 cut(s) 414, 771
MlyI GAGTC 2 cut(s) 19, 1490
Mox20I TGGCCA 2 cut(s) 414, 771
Mph1103I ATGCAT 2 cut(s) 337, 1126
MroXI GAANNNNTTC 3 cut(s) 794, 844, 1215
MscI TGGCCA 2 cut(s) 414, 771
MseI TTAA 5 cut(s) 158, 867, 1167, 1176, 1359
Msp20I TGGCCA 2 cut(s) 414, 771
MspA1I CMGCKG 2 cut(s) 818, 911
MspCI CTTAAG 1 cut(s) 866
MspI CCGG 8 cut(s) 17, 162, 233, 288, 439, 453, 849, 938
MspR9I CCNGG 5 cut(s) 18, 163, 234, 289, 1017
MunI CAATTG 3 cut(s) 721, 1220, 1308
Mva1269I GAATGC 2 cut(s) 753, 1282
MvaI CCWGG 1 cut(s) 1017
MvnI CGCG 1 cut(s) 818
MwoI GCNNNNNNNGC 2 cut(s) 136, 1499
NciI CCSGG 4 cut(s) 18, 163, 234, 289
NdeII GATC 4 cut(s) 280, 396, 826, 1522
NlaIII CATG 8 cut(s) 71, 479, 776, 1054, 1186, 1347, 1397, 1445
NlaIV GGNNCC 3 cut(s) 936, 1339, 1497
NmuCI GTSAC 3 cut(s) 262, 886, 1513
NsiI ATGCAT 2 cut(s) 337, 1126
NspI RCATGY 2 cut(s) 1186, 1397
PaqCI CACCTGC 1 cut(s) 214
PceI AGGCCT 2 cut(s) 152, 311
PciI ACATGT 1 cut(s) 1182
PctI GAATGC 2 cut(s) 753, 1282
PdmI GAANNNNTTC 3 cut(s) 794, 844, 1215
PfeI GAWTC 3 cut(s) 80, 170, 472
Pfl23II CGTACG 1 cut(s) 270
PkrI GCNGC 3 cut(s) 10, 451, 913
PleI GAGTC 2 cut(s) 19, 1489
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 2 cut(s) 19, 1489
Ppu21I YACGTR 1 cut(s) 262
PscI ACATGT 1 cut(s) 1182
Psp124BI GAGCTC 1 cut(s) 587
Psp1406I AACGTT 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 1015
PspCI CACGTG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 1015
PspLI CGTACG 1 cut(s) 270
PspN4I GGNNCC 3 cut(s) 936, 1339, 1497
PspPI GGNCC 4 cut(s) 344, 492, 935, 1225
PstNI CAGNNNCTG 1 cut(s) 421
RsaI GTAC 7 cut(s) 272, 419, 521, 635, 960, 1196, 1403
RsaNI GTAC 7 cut(s) 271, 418, 520, 634, 959, 1195, 1402
SacI GAGCTC 1 cut(s) 587
SacII CCGCGG 1 cut(s) 819
SaqAI TTAA 5 cut(s) 158, 867, 1167, 1176, 1359
SatI GCNGC 3 cut(s) 9, 450, 912
Sau3AI GATC 4 cut(s) 280, 396, 826, 1522
Sau96I GGNCC 4 cut(s) 344, 492, 935, 1225
ScaI AGTACT 1 cut(s) 1403
SchI GAGTC 2 cut(s) 19, 1490
ScrFI CCNGG 5 cut(s) 18, 163, 234, 289, 1017
SduI GDGCHC 5 cut(s) 168, 394, 587, 1307, 1415
SfaNI GCATC 2 cut(s) 322, 1111
SfcI CTRYAG 2 cut(s) 670, 915
Sfr303I CCGCGG 1 cut(s) 819
SgrBI CCGCGG 1 cut(s) 819
SinI GGWCC 3 cut(s) 344, 935, 1225
SmlI CTYRAG 2 cut(s) 866, 1006
SmoI CTYRAG 2 cut(s) 866, 1006
SpeI ACTAGT 1 cut(s) 242
Sse9I AATT 7 cut(s) 372, 721, 1042, 1212, 1220, 1308, 1365
SseBI AGGCCT 2 cut(s) 152, 311
SsiI CCGC 6 cut(s) 434, 816, 818, 822, 909, 1493
SspMI CTAG 7 cut(s) 213, 243, 659, 758, 800, 1032, 1422
SstI GAGCTC 1 cut(s) 587
StuI AGGCCT 2 cut(s) 152, 311
StyD4I CCNGG 5 cut(s) 16, 161, 232, 287, 1015
StyI CCWWGG 4 cut(s) 312, 382, 799, 1114
TaaI ACNGT 5 cut(s) 33, 64, 275, 1324, 1519
TaiI ACGT 3 cut(s) 264, 272, 488
TaqI TCGA 2 cut(s) 104, 118
TasI AATT 7 cut(s) 372, 721, 1042, 1212, 1220, 1308, 1365
TatI WGTACW 3 cut(s) 633, 958, 1401
TfiI GAWTC 3 cut(s) 80, 170, 472
Tru1I TTAA 5 cut(s) 158, 867, 1167, 1176, 1359
Tru9I TTAA 5 cut(s) 158, 867, 1167, 1176, 1359
TscAI CASTG 6 cut(s) 310, 859, 1066, 1195, 1420, 1524
TseFI GTSAC 3 cut(s) 262, 886, 1513
TseI GCWGC 3 cut(s) 8, 449, 911
Tsp45I GTSAC 3 cut(s) 262, 886, 1513
TspDTI ATGAA 7 cut(s) 56, 464, 531, 582, 603, 789, 1307
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 6 cut(s) 310, 859, 1066, 1195, 1420, 1524
Vha464I CTTAAG 1 cut(s) 866
VneI GTGCAC 1 cut(s) 390
VpaK11BI GGWCC 3 cut(s) 344, 935, 1225
XagI CCTNNNNNAGG 1 cut(s) 784
XceI RCATGY 2 cut(s) 1186, 1397
XmaJI CCTAGG 1 cut(s) 799
XmnI GAANNNNTTC 3 cut(s) 794, 844, 1215
XspI CTAG 7 cut(s) 213, 243, 659, 758, 800, 1032, 1422
ZrmI AGTACT 1 cut(s) 1403
Zsp2I ATGCAT 2 cut(s) 337, 1126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.