Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0002395

Physical Location & Seq

Genomic Coordinates Reverse (-)

15194786 .. 15197539

Gene Structure 2754 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG164100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGGAAGCTGCAATGCCGGGAAAGACTCTACTGTGGATTATGATTTGTAGCTTGTTTATCACAGTTCATGTTTACGGAGAATCCTCTCAGATGGGTCATTTTTCGAGGAGTGATTATCGAGACCAAATGCGAAGACTGCAAGAGTTCAAGGCCTCATTAACCGGGCACGATTCGGGTTCTTTTGTTGCACCATCTTCAGTCTCACCTGCTCCTAGCCCTAACCCTAACCCTCCGGGGAGGACTAGTCCTCGTGTGTATCACGTGACAACGTACGGTGCTGATCCTACCGGGAACAGGGACAGCACTGAGGCCTTGGCTCAGGCATTTGCAGATGCATTTAGAGGTCCATCAGAAGGGTTCTTGATGGAGGGAATTAGCAACCTTGGTGGTGCACAGATCAATCTTGAAGGTGGCCAGTACCTGATTAGCAAACCGCTCCGGCTTCCTGCTGCCGGAGTAGGAAACCTTATGATTCATGGAGGAACGTTGAGGGCCTCAGATAACTTCCCGACTGATGGGTACTTGATTGATTTATCTACTTCATCATCATCATCCCCCAACAAGAATGAAAAAAACAAAGAGAGCTCATCATCAATGTCTGCACAGCTTTCTTCATCATCCTACAACTATGAGTACATAACCCTAAAAGACCTTATGCTAGACGCCAACTACAGAGGTGGGGGCATTTCAGTCATAAACTCACTCAGAACCAGCATAGACAATTGCTACATAACCCATTTCAACACCGATGGCATTCTAGTCCAAAGTGGCCATGAAACCTACATCAGGAACTCCTTCCTAGGCCAGCACATCACCGCGGGCGGTGATCACAACGAAAGAAACTTTTCCGGCACTGCAATCAACCTTAAGGGAAATGACAATGCTGTGACAGATGTGGTGATTTTCTCCGCTGCTGTAGGTATAATGATTTCGGGACCGGCTAATATACTTTCGGGTGTACATTGCTACAATAAGGCCACAGGTTTCGGAGGTGTTGGGATTTACTTGAGATTGCCTGGTTTGACACAGACTAGGATTGTCAATTCTTACATGGACTACACTGGAATAGTTGCTGAAGACCCAGTTCAGCTTGATATATCAAACAGCTTCTTCCTTGGTGATGCATACATTGTGTTGAAATCAATAAAGGGTGTTGCCAATGGGGTTAATATAGTTAACAACATGTTCACTGGGTACAATAAGGGGATTGAAATTGTTCAATTGGACCAATCAAATGGGGGTTTCAAGGAGATTGAGCAAGTTGTTGTGGACAGGAATGCTGTGAGGGGGATGAACATAAAGGGCACAATTGGAAGGAGAACTGTGCAAGGCAATGGAACCTCATGGAATATAGACTTTAACCCAATTCTTCTATTTCCTAATCTTATTAGGCATGTGCAGTACTCTTTGAGCACTGGTGCTAGAAATGTGTTCCCCAACCATGCCCTGAGGAATGTATCAGATAATCGGGTCGTGATTGAGTCAGATGTGGCGGTTCCAGCTTCGGTTTTTGTGACAGTGGATCAAGGAATGTCAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004650 GO:0007049 GO:0007275 GO:0008150 GO:0009555 GO:0009556 GO:0009653 GO:0009987 GO:0010208 GO:0010584 GO:0010927 GO:0016043 GO:0016787 GO:0016798 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0030198 GO:0032501 GO:0032502 GO:0032989 GO:0034293 GO:0043062 GO:0043934 GO:0044085 GO:0044703 GO:0045229 GO:0048229 GO:0048236 GO:0048646 GO:0048856 GO:0048869 GO:0051321 GO:0051704 GO:0071840 GO:0085029 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pectate_lyase_3

Protein Analysis

513

Amino Acids

55.28

Weight (kDa)

6.18

Isoelectric Point (pI)

39.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	88 - 329	2.2e-08	Rhamnogalacturonase A/epimerase, pectate lyase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002395)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050 AT4G20050
fragaria_vesca	FvH4_3g12840 FvH4_6g36530
malus_domestica	MD05G1247500.v1.1 MD09G1167600.v1.1 MD10G1228300.v1.1
prunus_persica	Prupe.3G025600_v2.0.a1 Prupe.4G116600_v2.0.a1
pyrus_communis	pycom05g22350 pycom05g22360 pycom09g08510 pycom10g19190
rosa_chinensis	RchiOBHm_Chr2g0148991 RchiOBHm_Chr5g0020781
rosa_laevigata	RLG00000020361 RLG00000032562
rosa_multiflora	Rmu_co8368081.1_g000001 Rmu_co8378405.1_g000001 Rmu_sc0001397.1_g000010 Rmu_sc0002527.1_g000006 Rmu_sc0002527.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057520 Rroxscaffold_2G00098530
rosa_rugosa	Rorug02G0408600 Rorug05G0062800 Rorug05G0062900
rosa_samantha	Rh2AG467800 Rh2BG480400 Rh2CG454200 Rh2DG489300 Rh5AG152800 Rh5BG152500 Rh5CG164100 Rh5DG152000
rosa_wichuraiana	Rw2G038110 Rw5G013550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	214
Acc36I	ACCTGC	1 cut(s)	214
AccBSI	CCGCTC	1 cut(s)	436
AccII	CGCG	1 cut(s)	818
AciI	CCGC	6 cut(s)	434, 816, 818, 822, 909, 1493
AclI	AACGTT	1 cut(s)	485
AclWI	GGATC	2 cut(s)	275, 1530
AcoI	YGGCCR	2 cut(s)	412, 769
AcuI	CTGAAG	2 cut(s)	180, 1095
AcvI	CACGTG	1 cut(s)	262
AcyI	GRCGYC	1 cut(s)	663
AfaI	GTAC	7 cut(s)	272, 419, 521, 635, 960, 1196, 1403
AfiI	CCNNNNNNNGG	5 cut(s)	294, 353, 452, 515, 786
AflII	CTTAAG	1 cut(s)	866
AflIII	ACRYGT	1 cut(s)	1182
AgsI	TTSAA	7 cut(s)	148, 407, 742, 1138, 1211, 1220, 1246
AhlI	ACTAGT	1 cut(s)	242
AjnI	CCWGG	1 cut(s)	1015
AluBI	AGCT	7 cut(s)	8, 51, 585, 607, 1090, 1107, 1502
AluI	AGCT	7 cut(s)	8, 51, 585, 607, 1090, 1107, 1502
Alw21I	GWGCWC	3 cut(s)	394, 587, 1415
Alw26I	GTCTC	2 cut(s)	114, 205
Alw44I	GTGCAC	1 cut(s)	390
AlwI	GGATC	2 cut(s)	275, 1530
AlwNI	CAGNNNCTG	1 cut(s)	421
AoxI	GGCC	7 cut(s)	150, 309, 412, 492, 769, 802, 975
ApaLI	GTGCAC	1 cut(s)	390
ApeKI	GCWGC	3 cut(s)	8, 449, 911
Asp700I	GAANNNNTTC	3 cut(s)	794, 844, 1215
AspA2I	CCTAGG	1 cut(s)	799
AspS9I	GGNCC	4 cut(s)	344, 492, 935, 1225
AsuC2I	CCSGG	4 cut(s)	18, 163, 234, 289
AsuHPI	GGTGA	5 cut(s)	195, 805, 836, 910, 1130
AvaII	GGWCC	3 cut(s)	344, 935, 1225
AvrII	CCTAGG	1 cut(s)	799
AxyI	CCTNAGG	1 cut(s)	1448
BaeGI	GKGCMC	3 cut(s)	168, 394, 1307
BalI	TGGCCA	2 cut(s)	414, 771
BanII	GRGCYC	1 cut(s)	587
BauI	CACGAG	1 cut(s)	249
BbrPI	CACGTG	1 cut(s)	262
BbsI	GAAGAC	2 cut(s)	139, 1083
Bbv12I	GWGCWC	3 cut(s)	394, 587, 1415
BbvI	GCAGC	2 cut(s)	436, 898
BccI	CCATC	6 cut(s)	85, 199, 355, 358, 509, 743
BciT130I	CCWGG	1 cut(s)	1017
BclI	TGATCA	1 cut(s)	826
BcnI	CCSGG	4 cut(s)	18, 163, 234, 289
BcoDI	GTCTC	2 cut(s)	114, 205
BcuI	ACTAGT	1 cut(s)	242
BfaI	CTAG	7 cut(s)	213, 243, 659, 758, 800, 1032, 1422
BfmI	CTRYAG	2 cut(s)	670, 915
BfrI	CTTAAG	1 cut(s)	866
BfuAI	ACCTGC	1 cut(s)	214
BisI	GCNGC	3 cut(s)	9, 450, 912
BlnI	CCTAGG	1 cut(s)	799
BlsI	GCNGC	3 cut(s)	10, 451, 913
BmcAI	AGTACT	1 cut(s)	1403
Bme1390I	CCNGG	5 cut(s)	18, 163, 234, 289, 1017
Bme18I	GGWCC	3 cut(s)	344, 935, 1225
BmgT120I	GGNCC	4 cut(s)	344, 492, 935, 1225
BmiI	GGNNCC	3 cut(s)	936, 1339, 1497
BmrFI	CCNGG	5 cut(s)	18, 163, 234, 289, 1017
BmrI	ACTGGG	2 cut(s)	1076, 1200
BmsI	GCATC	2 cut(s)	322, 1111
BmuI	ACTGGG	2 cut(s)	1076, 1200
BpiI	GAAGAC	2 cut(s)	139, 1083
Bpu10I	CCTNAGC	1 cut(s)	318
BpuEI	CTTGAG	1 cut(s)	1027
BpuMI	CCSGG	4 cut(s)	18, 163, 234, 289
BsaAI	YACGTR	1 cut(s)	262
BsaHI	GRCGYC	1 cut(s)	663
BsaI	GGTCTC	1 cut(s)	114
BsaJI	CCNNGG	6 cut(s)	233, 312, 382, 799, 816, 1114
BsaXI	ACNNNNNCTCC	2 cut(s)	229, 259
Bsc4I	CCNNNNNNNGG	5 cut(s)	294, 353, 452, 515, 786
Bse118I	RCCGGY	1 cut(s)	937
Bse1I	ACTGG	5 cut(s)	415, 1066, 1082, 1195, 1420
Bse21I	CCTNAGG	1 cut(s)	1448
Bse3DI	GCAATG	3 cut(s)	18, 961, 1339
BseBI	CCWGG	1 cut(s)	1017
BseDI	CCNNGG	6 cut(s)	233, 312, 382, 799, 816, 1114
BseGI	GGATG	3 cut(s)	551, 617, 1296
BseLI	CCNNNNNNNGG	5 cut(s)	294, 353, 452, 515, 786
BseMI	GCAATG	3 cut(s)	18, 961, 1339
BseMII	CTCAG	6 cut(s)	101, 297, 332, 510, 718, 1439
BseNI	ACTGG	5 cut(s)	415, 1066, 1082, 1195, 1420
BseRI	GAGGAG	1 cut(s)	121
BseSI	GKGCMC	3 cut(s)	168, 394, 1307
BseXI	GCAGC	2 cut(s)	436, 898
BsgI	GTGCAG	2 cut(s)	585, 1418
Bsh1236I	CGCG	1 cut(s)	818
BshFI	GGCC	7 cut(s)	152, 311, 414, 494, 771, 804, 977
BsiHKAI	GWGCWC	3 cut(s)	394, 587, 1415
BsiSI	CCGG	8 cut(s)	17, 162, 233, 288, 439, 453, 849, 938
BsiWI	CGTACG	1 cut(s)	270
BslFI	GGGAC	2 cut(s)	311, 948
BslI	CCNNNNNNNGG	5 cut(s)	294, 353, 452, 515, 786
BsmAI	GTCTC	2 cut(s)	114, 205
BsmFI	GGGAC	2 cut(s)	311, 948
BsmI	GAATGC	2 cut(s)	753, 1282
BsnI	GGCC	7 cut(s)	152, 311, 414, 494, 771, 804, 977
Bso31I	GGTCTC	1 cut(s)	114
Bsp1286I	GDGCHC	5 cut(s)	168, 394, 587, 1307, 1415
Bsp1407I	TGTACA	1 cut(s)	958
Bsp143I	GATC	4 cut(s)	280, 396, 826, 1522
BspACI	CCGC	6 cut(s)	434, 816, 818, 822, 909, 1493
BspANI	GGCC	7 cut(s)	152, 311, 414, 494, 771, 804, 977
BspCNI	CTCAG	6 cut(s)	100, 298, 331, 509, 717, 1440
BspFNI	CGCG	1 cut(s)	818
BspLI	GGNNCC	3 cut(s)	936, 1339, 1497
BspMI	ACCTGC	1 cut(s)	214
BspPI	GGATC	2 cut(s)	275, 1530
BspTI	CTTAAG	1 cut(s)	866
BspTNI	GGTCTC	1 cut(s)	114
BsrBI	CCGCTC	1 cut(s)	436
BsrDI	GCAATG	3 cut(s)	18, 961, 1339
BsrFI	RCCGGY	1 cut(s)	937
BsrGI	TGTACA	1 cut(s)	958
BsrI	ACTGG	5 cut(s)	415, 1066, 1082, 1195, 1420
BssAI	RCCGGY	1 cut(s)	937
BssECI	CCNNGG	6 cut(s)	233, 312, 382, 799, 816, 1114
BssMI	GATC	4 cut(s)	280, 396, 826, 1522
BssNI	GRCGYC	1 cut(s)	663
BssSI	CACGAG	1 cut(s)	249
BssT1I	CCWWGG	4 cut(s)	312, 382, 799, 1114
Bst2BI	CACGAG	1 cut(s)	249
Bst2UI	CCWGG	1 cut(s)	1017
Bst4CI	ACNGT	5 cut(s)	33, 64, 275, 1324, 1519
BstACI	GRCGYC	1 cut(s)	663
BstAFI	CTTAAG	1 cut(s)	866
BstAUI	TGTACA	1 cut(s)	958
BstBAI	YACGTR	1 cut(s)	262
BstC8I	GCNNGC	2 cut(s)	806, 820
BstDEI	CTNAG	6 cut(s)	87, 306, 318, 496, 704, 1448
BstDSI	CCRYGG	1 cut(s)	816
BstENI	CCTNNNNNAGG	1 cut(s)	784
BstF5I	GGATG	3 cut(s)	551, 617, 1296
BstFNI	CGCG	1 cut(s)	818
BstKTI	GATC	4 cut(s)	283, 399, 829, 1525
BstMAI	GTCTC	2 cut(s)	114, 205
BstMBI	GATC	4 cut(s)	280, 396, 826, 1522
BstMWI	GCNNNNNNNGC	2 cut(s)	136, 1499
BstNI	CCWGG	1 cut(s)	1017
BstNSI	RCATGY	2 cut(s)	1186, 1397
BstSCI	CCNGG	5 cut(s)	16, 161, 232, 287, 1015
BstSFI	CTRYAG	2 cut(s)	670, 915
BstSLI	GKGCMC	3 cut(s)	168, 394, 1307
BstUI	CGCG	1 cut(s)	818
BstV1I	GCAGC	2 cut(s)	436, 898
BstV2I	GAAGAC	2 cut(s)	139, 1083
BstXI	CCANNNNNNTGG	1 cut(s)	1235
Bsu36I	CCTNAGG	1 cut(s)	1448
BsuRI	GGCC	7 cut(s)	152, 311, 414, 494, 771, 804, 977
BtgI	CCRYGG	1 cut(s)	816
BtsCI	GGATG	3 cut(s)	551, 617, 1296
BtsI	GCAGTG	1 cut(s)	852
BtsIMutI	CAGTG	6 cut(s)	303, 852, 1059, 1188, 1413, 1524
BveI	ACCTGC	1 cut(s)	214
Cac8I	GCNNGC	2 cut(s)	806, 820
CaiI	CAGNNNCTG	1 cut(s)	421
Cfr10I	RCCGGY	1 cut(s)	937
Cfr13I	GGNCC	4 cut(s)	344, 492, 935, 1225
Cfr42I	CCGCGG	1 cut(s)	819
CseI	GACGC	1 cut(s)	671
Csp6I	GTAC	7 cut(s)	271, 418, 520, 634, 959, 1195, 1402
CspCI	CAANNNNNGTGG	2 cut(s)	367, 402
CviAII	CATG	8 cut(s)	68, 476, 773, 1051, 1183, 1344, 1394, 1442
CviQI	GTAC	7 cut(s)	271, 418, 520, 634, 959, 1195, 1402
DdeI	CTNAG	6 cut(s)	87, 306, 318, 496, 704, 1448
DpnI	GATC	4 cut(s)	282, 398, 828, 1524
DpnII	GATC	4 cut(s)	280, 396, 826, 1522
EaeI	YGGCCR	2 cut(s)	412, 769
Ecl136II	GAGCTC	1 cut(s)	585
Eco130I	CCWWGG	4 cut(s)	312, 382, 799, 1114
Eco147I	AGGCCT	2 cut(s)	152, 311
Eco24I	GRGCYC	1 cut(s)	587
Eco31I	GGTCTC	1 cut(s)	114
Eco47I	GGWCC	3 cut(s)	344, 935, 1225
Eco53kI	GAGCTC	1 cut(s)	585
Eco57I	CTGAAG	2 cut(s)	180, 1095
Eco72I	CACGTG	1 cut(s)	262
Eco81I	CCTNAGG	1 cut(s)	1448
EcoICRI	GAGCTC	1 cut(s)	585
EcoNI	CCTNNNNNAGG	1 cut(s)	784
EcoO109I	RGGNCCY	1 cut(s)	492
EcoRII	CCWGG	1 cut(s)	1015
EcoT14I	CCWWGG	4 cut(s)	312, 382, 799, 1114
EcoT22I	ATGCAT	2 cut(s)	337, 1126
EcoT38I	GRGCYC	1 cut(s)	587
ErhI	CCWWGG	4 cut(s)	312, 382, 799, 1114
FaeI	CATG	8 cut(s)	71, 479, 776, 1054, 1186, 1347, 1397, 1445
FaqI	GGGAC	2 cut(s)	311, 948
FatI	CATG	8 cut(s)	67, 475, 772, 1050, 1182, 1343, 1393, 1441
FauI	CCCGC	1 cut(s)	811
FbaI	TGATCA	1 cut(s)	826
Fnu4HI	GCNGC	3 cut(s)	9, 450, 912
FokI	GGATG	3 cut(s)	538, 604, 1303
FriOI	GRGCYC	1 cut(s)	587
Fsp4HI	GCNGC	3 cut(s)	9, 450, 912
FspBI	CTAG	7 cut(s)	213, 243, 659, 758, 800, 1032, 1422
GluI	GCNGC	3 cut(s)	9, 450, 912
HaeIII	GGCC	7 cut(s)	152, 311, 414, 494, 771, 804, 977
HapII	CCGG	8 cut(s)	17, 162, 233, 288, 439, 453, 849, 938
HgaI	GACGC	1 cut(s)	671
Hin1I	GRCGYC	1 cut(s)	663
Hin1II	CATG	8 cut(s)	71, 479, 776, 1054, 1186, 1347, 1397, 1445
HincII	GTYRAC	1 cut(s)	1177
HindII	GTYRAC	1 cut(s)	1177
HinfI	GANTC	5 cut(s)	25, 80, 170, 472, 1481
HpaI	GTTAAC	1 cut(s)	1177
HpaII	CCGG	8 cut(s)	17, 162, 233, 288, 439, 453, 849, 938
HphI	GGTGA	5 cut(s)	195, 805, 836, 910, 1130
Hpy166II	GTNNAC	6 cut(s)	73, 392, 959, 1177, 1188, 1270
Hpy188I	TCNGA	7 cut(s)	90, 352, 499, 707, 989, 1462, 1486
Hpy188III	TCNNGA	7 cut(s)	119, 361, 404, 508, 787, 933, 1474
Hpy8I	GTNNAC	6 cut(s)	73, 392, 959, 1177, 1188, 1270
HpyAV	CCTTC	4 cut(s)	347, 401, 805, 1308
HpyCH4III	ACNGT	5 cut(s)	33, 64, 275, 1324, 1519
HpyCH4IV	ACGT	3 cut(s)	261, 269, 485
HpyF10VI	GCNNNNNNNGC	2 cut(s)	136, 1499
HpyF3I	CTNAG	6 cut(s)	87, 306, 318, 496, 704, 1448
HpySE526I	ACGT	3 cut(s)	261, 269, 485
Hsp92I	GRCGYC	1 cut(s)	663
Hsp92II	CATG	8 cut(s)	71, 479, 776, 1054, 1186, 1347, 1397, 1445
Ksp22I	TGATCA	1 cut(s)	826
KspAI	GTTAAC	1 cut(s)	1177
KspI	CCGCGG	1 cut(s)	819
Kzo9I	GATC	4 cut(s)	280, 396, 826, 1522
LmnI	GCTCC	2 cut(s)	214, 441
Lsp1109I	GCAGC	2 cut(s)	436, 898
LweI	GCATC	2 cut(s)	322, 1111
MaeI	CTAG	7 cut(s)	213, 243, 659, 758, 800, 1032, 1422
MaeII	ACGT	3 cut(s)	261, 269, 485
MaeIII	GTNAC	3 cut(s)	262, 886, 1513
MalI	GATC	4 cut(s)	282, 398, 828, 1524
MbiI	CCGCTC	1 cut(s)	436
MboI	GATC	4 cut(s)	280, 396, 826, 1522
MboII	GAAGA	6 cut(s)	144, 186, 603, 1088, 1102, 1361
MfeI	CAATTG	3 cut(s)	721, 1220, 1308
MhlI	GDGCHC	5 cut(s)	168, 394, 587, 1307, 1415
MlsI	TGGCCA	2 cut(s)	414, 771
MluCI	AATT	7 cut(s)	372, 721, 1042, 1212, 1220, 1308, 1365
MluNI	TGGCCA	2 cut(s)	414, 771
MlyI	GAGTC	2 cut(s)	19, 1490
Mox20I	TGGCCA	2 cut(s)	414, 771
Mph1103I	ATGCAT	2 cut(s)	337, 1126
MroXI	GAANNNNTTC	3 cut(s)	794, 844, 1215
MscI	TGGCCA	2 cut(s)	414, 771
MseI	TTAA	5 cut(s)	158, 867, 1167, 1176, 1359
Msp20I	TGGCCA	2 cut(s)	414, 771
MspA1I	CMGCKG	2 cut(s)	818, 911
MspCI	CTTAAG	1 cut(s)	866
MspI	CCGG	8 cut(s)	17, 162, 233, 288, 439, 453, 849, 938
MspR9I	CCNGG	5 cut(s)	18, 163, 234, 289, 1017
MunI	CAATTG	3 cut(s)	721, 1220, 1308
Mva1269I	GAATGC	2 cut(s)	753, 1282
MvaI	CCWGG	1 cut(s)	1017
MvnI	CGCG	1 cut(s)	818
MwoI	GCNNNNNNNGC	2 cut(s)	136, 1499
NciI	CCSGG	4 cut(s)	18, 163, 234, 289
NdeII	GATC	4 cut(s)	280, 396, 826, 1522
NlaIII	CATG	8 cut(s)	71, 479, 776, 1054, 1186, 1347, 1397, 1445
NlaIV	GGNNCC	3 cut(s)	936, 1339, 1497
NmuCI	GTSAC	3 cut(s)	262, 886, 1513
NsiI	ATGCAT	2 cut(s)	337, 1126
NspI	RCATGY	2 cut(s)	1186, 1397
PaqCI	CACCTGC	1 cut(s)	214
PceI	AGGCCT	2 cut(s)	152, 311
PciI	ACATGT	1 cut(s)	1182
PctI	GAATGC	2 cut(s)	753, 1282
PdmI	GAANNNNTTC	3 cut(s)	794, 844, 1215
PfeI	GAWTC	3 cut(s)	80, 170, 472
Pfl23II	CGTACG	1 cut(s)	270
PkrI	GCNGC	3 cut(s)	10, 451, 913
PleI	GAGTC	2 cut(s)	19, 1489
PmaCI	CACGTG	1 cut(s)	262
PmlI	CACGTG	1 cut(s)	262
PpsI	GAGTC	2 cut(s)	19, 1489
Ppu21I	YACGTR	1 cut(s)	262
PscI	ACATGT	1 cut(s)	1182
Psp124BI	GAGCTC	1 cut(s)	587
Psp1406I	AACGTT	1 cut(s)	485
Psp6I	CCWGG	1 cut(s)	1015
PspCI	CACGTG	1 cut(s)	262
PspGI	CCWGG	1 cut(s)	1015
PspLI	CGTACG	1 cut(s)	270
PspN4I	GGNNCC	3 cut(s)	936, 1339, 1497
PspPI	GGNCC	4 cut(s)	344, 492, 935, 1225
PstNI	CAGNNNCTG	1 cut(s)	421
RsaI	GTAC	7 cut(s)	272, 419, 521, 635, 960, 1196, 1403
RsaNI	GTAC	7 cut(s)	271, 418, 520, 634, 959, 1195, 1402
SacI	GAGCTC	1 cut(s)	587
SacII	CCGCGG	1 cut(s)	819
SaqAI	TTAA	5 cut(s)	158, 867, 1167, 1176, 1359
SatI	GCNGC	3 cut(s)	9, 450, 912
Sau3AI	GATC	4 cut(s)	280, 396, 826, 1522
Sau96I	GGNCC	4 cut(s)	344, 492, 935, 1225
ScaI	AGTACT	1 cut(s)	1403
SchI	GAGTC	2 cut(s)	19, 1490
ScrFI	CCNGG	5 cut(s)	18, 163, 234, 289, 1017
SduI	GDGCHC	5 cut(s)	168, 394, 587, 1307, 1415
SfaNI	GCATC	2 cut(s)	322, 1111
SfcI	CTRYAG	2 cut(s)	670, 915
Sfr303I	CCGCGG	1 cut(s)	819
SgrBI	CCGCGG	1 cut(s)	819
SinI	GGWCC	3 cut(s)	344, 935, 1225
SmlI	CTYRAG	2 cut(s)	866, 1006
SmoI	CTYRAG	2 cut(s)	866, 1006
SpeI	ACTAGT	1 cut(s)	242
Sse9I	AATT	7 cut(s)	372, 721, 1042, 1212, 1220, 1308, 1365
SseBI	AGGCCT	2 cut(s)	152, 311
SsiI	CCGC	6 cut(s)	434, 816, 818, 822, 909, 1493
SspMI	CTAG	7 cut(s)	213, 243, 659, 758, 800, 1032, 1422
SstI	GAGCTC	1 cut(s)	587
StuI	AGGCCT	2 cut(s)	152, 311
StyD4I	CCNGG	5 cut(s)	16, 161, 232, 287, 1015
StyI	CCWWGG	4 cut(s)	312, 382, 799, 1114
TaaI	ACNGT	5 cut(s)	33, 64, 275, 1324, 1519
TaiI	ACGT	3 cut(s)	264, 272, 488
TaqI	TCGA	2 cut(s)	104, 118
TasI	AATT	7 cut(s)	372, 721, 1042, 1212, 1220, 1308, 1365
TatI	WGTACW	3 cut(s)	633, 958, 1401
TfiI	GAWTC	3 cut(s)	80, 170, 472
Tru1I	TTAA	5 cut(s)	158, 867, 1167, 1176, 1359
Tru9I	TTAA	5 cut(s)	158, 867, 1167, 1176, 1359
TscAI	CASTG	6 cut(s)	310, 859, 1066, 1195, 1420, 1524
TseFI	GTSAC	3 cut(s)	262, 886, 1513
TseI	GCWGC	3 cut(s)	8, 449, 911
Tsp45I	GTSAC	3 cut(s)	262, 886, 1513
TspDTI	ATGAA	7 cut(s)	56, 464, 531, 582, 603, 789, 1307
TspGWI	ACGGA	1 cut(s)	90
TspRI	CASTG	6 cut(s)	310, 859, 1066, 1195, 1420, 1524
Vha464I	CTTAAG	1 cut(s)	866
VneI	GTGCAC	1 cut(s)	390
VpaK11BI	GGWCC	3 cut(s)	344, 935, 1225
XagI	CCTNNNNNAGG	1 cut(s)	784
XceI	RCATGY	2 cut(s)	1186, 1397
XmaJI	CCTAGG	1 cut(s)	799
XmnI	GAANNNNTTC	3 cut(s)	794, 844, 1215
XspI	CTAG	7 cut(s)	213, 243, 659, 758, 800, 1032, 1422
ZrmI	AGTACT	1 cut(s)	1403
Zsp2I	ATGCAT	2 cut(s)	337, 1126

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.