FvH4_3g15943

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004847
Physical Location & Seq
Reverse (-)
10046807 .. 10048216
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g15943.t1

Sequence Viewer

Length: 741 bp
ATGAGCCTACCGGAGAAGACAAGGGAGGACACACATGACAACCCAACAATGGAGGCCTCAAAAGAGGAATTGGCAAAGTTGGAGCAGCAAGTTGAGATCTTGGATGTCGTTCGTGTGCCAAGGAAAGGTCAGGAAGATTCGGACTTTGTTGCTCTCTGGGCTGGAAAAGAAGACATGGAGGTTTTTGCTCAAATCTTGGAGAAGTGGAAGGAAGGCCTCGATGGAGATGCCAAAGACCGATCAATGCTGGATGCATCTCTAGATTGCATTGGAGAAAGCTGTGAATTTGGGGTGTTCAGCTCTGTGCTTCTAAAACACTTGTTGGTCTCATTACCGGAGAGCAAAGAACGTGAAATGGTGATTAAGCAACTAAAAGCTGTACTGAAAAACCTTGCCGGAGATGAAAGAAACATTTCGGCTAAGAAGTTCATAGAGTTGGGATTTTTTTATTTGAAAAGTCAGAGCATGACAGGTGGTACTGGTGTTGTAGAAGCAGCTGATTATGGAACCATTTATCAGTTCAAGATGCTATTGATCAGATCTTTGGACAACTATGTTAAGCAGAGCGATAAACATGTGGATGTGTTGAAGCTGAAGATGAATATGTGTATGGAGAATTGGAAGCTGCAAGCTCCACCTTCCAGTTCTCCAAAGACATACTCATCTTCTGCTTCAACGCATCCGTATGTTTATCTGCTTGACATTACTGTTCAAAGACTCAGAAAGGTAACAAAATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

247

Amino Acids

27.91

Weight (kDa)

5.71

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 284, 734
AcuI CTGAAG 1 cut(s) 614
AfaI GTAC 2 cut(s) 381, 478
AfiI CCNNNNNNNGG 2 cut(s) 49, 125
AflIII ACRYGT 1 cut(s) 574
AgsI TTSAA 5 cut(s) 454, 523, 589, 675, 713
AluBI AGCT 7 cut(s) 279, 300, 377, 497, 592, 625, 632
AluI AGCT 7 cut(s) 279, 300, 377, 497, 592, 625, 632
Alw26I GTCTC 1 cut(s) 331
AoxI GGCC 2 cut(s) 54, 214
ApeKI GCWGC 3 cut(s) 85, 494, 625
ApoI RAATTY 2 cut(s) 284, 734
Asp700I GAANNNNTTC 1 cut(s) 412
AsuHPI GGTGA 1 cut(s) 370
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BbsI GAAGAC 2 cut(s) 23, 177
BbvI GCAGC 3 cut(s) 97, 506, 612
BccI CCATC 1 cut(s) 215
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BclI TGATCA 1 cut(s) 534
BcoDI GTCTC 1 cut(s) 331
BfaI CTAG 1 cut(s) 260
BglII AGATCT 2 cut(s) 96, 539
BisI GCNGC 3 cut(s) 86, 495, 626
BlsI GCNGC 3 cut(s) 87, 496, 627
BmiI GGNNCC 1 cut(s) 508
BmsI GCATC 5 cut(s) 217, 241, 263, 516, 688
BpiI GAAGAC 2 cut(s) 23, 177
BsaI GGTCTC 1 cut(s) 331
BsaJI CCNNGG 1 cut(s) 119
BsaWI WCCGGW 2 cut(s) 10, 334
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 125
Bse1I ACTGG 2 cut(s) 484, 642
BseDI CCNNGG 1 cut(s) 119
BseGI GGATG 4 cut(s) 109, 256, 586, 679
BseLI CCNNNNNNNGG 2 cut(s) 49, 125
BseMII CTCAG 1 cut(s) 733
BseNI ACTGG 2 cut(s) 484, 642
BseXI GCAGC 3 cut(s) 97, 506, 612
BshFI GGCC 2 cut(s) 56, 216
BsiSI CCGG 3 cut(s) 11, 335, 396
BslI CCNNNNNNNGG 2 cut(s) 49, 125
BsmAI GTCTC 1 cut(s) 331
BsnI GGCC 2 cut(s) 56, 216
Bso31I GGTCTC 1 cut(s) 331
Bsp143I GATC 4 cut(s) 96, 239, 534, 539
BspANI GGCC 2 cut(s) 56, 216
BspCNI CTCAG 1 cut(s) 732
BspLI GGNNCC 1 cut(s) 508
BspTNI GGTCTC 1 cut(s) 331
BsrI ACTGG 2 cut(s) 484, 642
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 4 cut(s) 96, 239, 534, 539
BssT1I CCWWGG 1 cut(s) 119
Bst4CI ACNGT 1 cut(s) 709
BstC8I GCNNGC 1 cut(s) 630
BstDEI CTNAG 2 cut(s) 420, 719
BstF5I GGATG 4 cut(s) 109, 256, 586, 679
BstKTI GATC 4 cut(s) 99, 242, 537, 542
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 4 cut(s) 96, 239, 534, 539
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstNSI RCATGY 1 cut(s) 578
BstV1I GCAGC 3 cut(s) 97, 506, 612
BstV2I GAAGAC 2 cut(s) 23, 177
BstX2I RGATCY 2 cut(s) 96, 539
BstYI RGATCY 2 cut(s) 96, 539
BsuRI GGCC 2 cut(s) 56, 216
BtsCI GGATG 4 cut(s) 109, 256, 586, 679
Cac8I GCNNGC 1 cut(s) 630
Csp6I GTAC 2 cut(s) 380, 477
CviAII CATG 4 cut(s) 35, 175, 466, 575
CviQI GTAC 2 cut(s) 380, 477
DdeI CTNAG 2 cut(s) 420, 719
DpnI GATC 4 cut(s) 98, 241, 536, 541
DpnII GATC 4 cut(s) 96, 239, 534, 539
Eco130I CCWWGG 1 cut(s) 119
Eco147I AGGCCT 2 cut(s) 56, 216
Eco31I GGTCTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 614
EcoT14I CCWWGG 1 cut(s) 119
EcoT22I ATGCAT 1 cut(s) 256
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 4 cut(s) 38, 178, 469, 578
FatI CATG 4 cut(s) 34, 174, 465, 574
FbaI TGATCA 1 cut(s) 534
Fnu4HI GCNGC 3 cut(s) 86, 495, 626
FokI GGATG 4 cut(s) 116, 263, 593, 666
Fsp4HI GCNGC 3 cut(s) 86, 495, 626
FspBI CTAG 1 cut(s) 260
GluI GCNGC 3 cut(s) 86, 495, 626
HaeIII GGCC 2 cut(s) 56, 216
HapII CCGG 3 cut(s) 11, 335, 396
Hin1II CATG 4 cut(s) 38, 178, 469, 578
HinfI GANTC 2 cut(s) 137, 717
HpaII CCGG 3 cut(s) 11, 335, 396
HphI GGTGA 1 cut(s) 370
Hpy188I TCNGA 5 cut(s) 142, 462, 539, 722, 740
Hpy188III TCNNGA 3 cut(s) 131, 260, 523
HpyAV CCTTC 3 cut(s) 202, 206, 648
HpyCH4III ACNGT 1 cut(s) 709
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 3 cut(s) 254, 267, 628
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 2 cut(s) 420, 719
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 4 cut(s) 38, 178, 469, 578
Ksp22I TGATCA 1 cut(s) 534
Kzo9I GATC 4 cut(s) 96, 239, 534, 539
LmnI GCTCC 2 cut(s) 82, 637
Lsp1109I GCAGC 3 cut(s) 97, 506, 612
LweI GCATC 5 cut(s) 217, 241, 263, 516, 688
MaeI CTAG 1 cut(s) 260
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 1 cut(s) 727
MalI GATC 4 cut(s) 98, 241, 536, 541
MboI GATC 4 cut(s) 96, 239, 534, 539
MboII GAAGA 5 cut(s) 28, 146, 182, 607, 657
MflI RGATCY 2 cut(s) 96, 539
MluCI AATT 4 cut(s) 68, 284, 616, 734
MlyI GAGTC 1 cut(s) 711
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 6 cut(s) 19, 46, 58, 67, 172, 227
Mph1103I ATGCAT 1 cut(s) 256
MroXI GAANNNNTTC 1 cut(s) 412
MseI TTAA 2 cut(s) 363, 558
MslI CAYNNNNRTG 2 cut(s) 579, 684
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 3 cut(s) 11, 335, 396
MwoI GCNNNNNNNGC 1 cut(s) 158
NdeII GATC 4 cut(s) 96, 239, 534, 539
NlaIII CATG 4 cut(s) 38, 178, 469, 578
NlaIV GGNNCC 1 cut(s) 508
NsiI ATGCAT 1 cut(s) 256
NspI RCATGY 1 cut(s) 578
PceI AGGCCT 2 cut(s) 56, 216
PciI ACATGT 1 cut(s) 574
PdmI GAANNNNTTC 1 cut(s) 412
PfeI GAWTC 1 cut(s) 137
PkrI GCNGC 3 cut(s) 87, 496, 627
PleI GAGTC 1 cut(s) 711
PpsI GAGTC 1 cut(s) 711
PscI ACATGT 1 cut(s) 574
PspN4I GGNNCC 1 cut(s) 508
PsuI RGATCY 2 cut(s) 96, 539
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 2 cut(s) 381, 478
RsaNI GTAC 2 cut(s) 380, 477
RseI CAYNNNNRTG 2 cut(s) 579, 684
SaqAI TTAA 2 cut(s) 363, 558
SatI GCNGC 3 cut(s) 86, 495, 626
Sau3AI GATC 4 cut(s) 96, 239, 534, 539
SchI GAGTC 1 cut(s) 711
SfaNI GCATC 5 cut(s) 217, 241, 263, 516, 688
SmiMI CAYNNNNRTG 2 cut(s) 579, 684
Sse9I AATT 4 cut(s) 68, 284, 616, 734
SseBI AGGCCT 2 cut(s) 56, 216
SspMI CTAG 1 cut(s) 260
StuI AGGCCT 2 cut(s) 56, 216
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 1 cut(s) 709
TaiI ACGT 1 cut(s) 352
TaqI TCGA 1 cut(s) 219
TaqII GACCGA 1 cut(s) 252
TasI AATT 4 cut(s) 68, 284, 616, 734
TatI WGTACW 1 cut(s) 379
TfiI GAWTC 1 cut(s) 137
Tru1I TTAA 2 cut(s) 363, 558
Tru9I TTAA 2 cut(s) 363, 558
TseI GCWGC 3 cut(s) 85, 494, 625
TspDTI ATGAA 3 cut(s) 417, 418, 614
TspGWI ACGGA 1 cut(s) 672
XapI RAATTY 2 cut(s) 284, 734
XbaI TCTAGA 1 cut(s) 259
XceI RCATGY 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 412
XspI CTAG 1 cut(s) 260
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.