Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0004847

Physical Location & Seq

Genomic Coordinates Reverse (-)

20817035 .. 20818609

Gene Structure 1575 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG206100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1575 bp

ATGGAGAATCAGCAGGAGAATCCACCGGAGAACCCACCGGCGAACAACCCAGAAGCAGTGGAGAATACTCTTATTGAATGCATTGAAGCAGCCACCAAGTTGTCGTCACCAGAGAATCAGCAGGAGGAGAACGGCCCAGAAGTACTGGAGAATACTCTTGTTGAATGCATTGAAACAGCCAACAAGCTGTCGTCTCTTCTTGCAAGAGCATTGAGAAGCCACAGAGATTTGTTCAAAGAATCAAGAATGGAGATCCCAGTCCCACCGGAGAAGGAGCCAACATTTCGGGAGATTCACATGCCTAAATGCATTGAAGCAGCGAACAAGTTGTCGTCTCTTCTAACAAGAACATGGTTCTTGCAAGGAACTCCCATTTCTGGCTCTGCAGGTTTTGCTTCCTCTCTGAAAAATCGTCTACGCGATCCAGAAACATCAACCATTTTCAACACTATCTGCTCCGATTTCCAAAAGGTGCACCAGTTGATGAAAACCCATAAGAGAACCCGACGCACACTGAAGTTCCAAACTCTTCTTGAGAGAGACATGGGTGTTCACGACCTCGTGCCTCGCATTCAGAAGGAGCTAAGAAGCATGGCCGCGGTTGAAGTTGCATTGAAAGGTGGATTTGAAGGTGAAGCGGTAAAAGAACGTCTGAAGAAGTGTAAGGAGAACACCCAAGGCTTGGAGCTGGAAGTTGAAATCTTGGAGGCTATTCGTGTGCCAGAGAAGAATGATGCGCAAGATTTAGACTTTGTTGCTATCTGGGGTGGAAAAGAAGAGATAGAGGTTTCTGGTGAAGTCTTGGACGAGTGGAACCGATTCTTGAATGAAGGGCTTGATAAAATTAACAATGCTCAGGAACAAGCACAAGTAGTCTCGGTTGAGAATCCAAGGATCACAGAATCGAAGGGAAACGAAATGCGGGCTTATACTGGAGGAAAAGAAGCATGTCAAGGCAAGAAGGGGGAACCAAGTCCAGTAATTTTGTCCAATTGGGTCAGGAAGAATCAAAGTAACCGGCCTCCGGTCTGGGACTCTCTCTGGCTTTGCTTGACTGATGCTGTGATGTTGGGGTGTCATGCCTCTGTGCTCCTCGAATTCTTGTTGGTCTCACTGCCAGAGAGCCAAGAAGGAGAGATGAAGATTGGGCAGCTTAAGGCTGCACTGGGAAAGCTTGATAAAGATGGAAGAAACATTGCAGCTAAGAGAGTCTTGGAGTTGGACCTCTTCAGGTTCCCAAGTCAGAACATGATTGGTGCTGCTGCTGGTGTTCAAGCTGCTGCTGCTGCTGCTGGTGTTCAAGCTGCTGCTGATGATGATAGAACAATTTACCAGTTGAAGAAGCTGTTTATTACAGTGAACTTGATGTTCCGTCATCCCATTTCTCTCGGGAAAGGAGACGAGCCTGTGGTTTCTCTGCAAAGCTATGCCGGACAGTGCACTAAACTAGTGAATGCGCTGAAGCAGAAATTGCGGATGCCTGAGGAGCACTGGAATGTGGAGGTGGAGAGAATGAAGAAGTCATGGGAGCCCCTAAACGTCAGTCGTCCCAGTTTGTTCAAAACAGTGGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

524

Amino Acids

58.59

Weight (kDa)

5.85

Isoelectric Point (pI)

48.22

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004847)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g15942 FvH4_3g15943
rosa_chinensis	RchiOBHm_Chr2g0132201 RchiOBHm_Chr5g0026881
rosa_laevigata	RLG00000032967
rosa_multiflora	Rmu_sc0000395.1_g000031 Rmu_sc0000395.1_g000032 Rmu_sc0014333.1_g000001 Rmu_ssc0000172.1_g000015
rosa_roxburghii	Rroxscaffold_5G00358840
rosa_samantha	Rh1CG019100 Rh1DG123800 Rh1DG123900 Rh1DG125900 Rh1DG133500 Rh2BG362300 Rh2BG531200 Rh2DG540200 Rh4AG122900 Rh4AG177700 Rh5BG186700 Rh5BG186800 Rh5CG206100 Rh5DG188300 Rh5DG188400 Rh6CG181800 Rh6CG232600 Rh6DG095900 Rh6DG173000 Rh6DG173100
rosa_wichuraiana	Rw5G017200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	738
Acc36I	ACCTGC	1 cut(s)	377
AccB7I	CCANNNNNTGG	1 cut(s)	682
AccI	GTMKAC	1 cut(s)	415
AccII	CGCG	2 cut(s)	420, 599
AciI	CCGC	5 cut(s)	597, 599, 638, 922, 1474
AclWI	GGATC	3 cut(s)	247, 416, 902
AcoI	YGGCCR	1 cut(s)	594
AcsI	RAATTY	1 cut(s)	1097
AcuI	CTGAAG	4 cut(s)	536, 674, 1213, 1481
AfaI	GTAC	1 cut(s)	144
AfiI	CCNNNNNNNGG	3 cut(s)	377, 682, 1024
AflII	CTTAAG	1 cut(s)	1154
AhlI	ACTAGT	1 cut(s)	1447
Alw21I	GWGCWC	4 cut(s)	477, 1092, 1442, 1491
Alw26I	GTCTC	6 cut(s)	198, 339, 534, 880, 1114, 1392
Alw44I	GTGCAC	2 cut(s)	473, 1438
AlwI	GGATC	3 cut(s)	247, 416, 902
Ama87I	CYCGRG	1 cut(s)	1388
AoxI	GGCC	3 cut(s)	133, 594, 1019
ApaLI	GTGCAC	2 cut(s)	473, 1438
ApoI	RAATTY	1 cut(s)	1097
ArsI	GACNNNNNNTTYG	2 cut(s)	314, 346
Asp700I	GAANNNNTTC	1 cut(s)	818
AspLEI	GCGC	2 cut(s)	739, 1459
AspS9I	GGNCC	2 cut(s)	134, 1222
AsuHPI	GGTGA	3 cut(s)	99, 644, 806
AvaI	CYCGRG	1 cut(s)	1388
AvaII	GGWCC	1 cut(s)	1222
AxyI	CCTNAGG	1 cut(s)	1482
BaeGI	GKGCMC	2 cut(s)	477, 1442
BanII	GRGCYC	1 cut(s)	1533
BauI	CACGAG	1 cut(s)	560
Bbv12I	GWGCWC	4 cut(s)	477, 1092, 1442, 1491
BccI	CCATC	1 cut(s)	1178
BceAI	ACGGC	1 cut(s)	148
BcoDI	GTCTC	6 cut(s)	198, 339, 534, 880, 1114, 1392
BcuI	ACTAGT	1 cut(s)	1447
BfaI	CTAG	1 cut(s)	1448
BfmI	CTRYAG	1 cut(s)	384
BfrI	CTTAAG	1 cut(s)	1154
BfuAI	ACCTGC	1 cut(s)	377
BmcAI	AGTACT	1 cut(s)	144
Bme18I	GGWCC	1 cut(s)	1222
BmeT110I	CYCGRG	1 cut(s)	1388
BmgT120I	GGNCC	2 cut(s)	134, 1222
BmiI	GGNNCC	5 cut(s)	276, 815, 969, 1235, 1530
BmrI	ACTGGG	3 cut(s)	251, 1175, 1545
BmsI	GCATC	3 cut(s)	724, 1048, 1467
BmuI	ACTGGG	3 cut(s)	251, 1175, 1545
BpmI	CTGGAG	2 cut(s)	167, 954
Bpu10I	CCTNAGC	1 cut(s)	855
BpuEI	CTTGAG	1 cut(s)	554
BsaI	GGTCTC	1 cut(s)	1114
BsaJI	CCNNGG	3 cut(s)	597, 676, 890
BsaWI	WCCGGW	3 cut(s)	25, 265, 1024
Bsc4I	CCNNNNNNNGG	3 cut(s)	377, 682, 1024
Bse118I	RCCGGY	2 cut(s)	37, 1017
Bse1I	ACTGG	9 cut(s)	150, 257, 478, 937, 977, 1170, 1333, 1496, 1551
Bse21I	CCTNAGG	1 cut(s)	1482
Bse3DI	GCAATG	1 cut(s)	1194
BseDI	CCNNGG	3 cut(s)	597, 676, 890
BseGI	GGATG	2 cut(s)	1375, 1482
BseLI	CCNNNNNNNGG	3 cut(s)	377, 682, 1024
BseMI	GCAATG	1 cut(s)	1194
BseMII	CTCAG	2 cut(s)	869, 1473
BseNI	ACTGG	9 cut(s)	150, 257, 478, 937, 977, 1170, 1333, 1496, 1551
BseRI	GAGGAG	3 cut(s)	140, 1082, 1499
BseSI	GKGCMC	2 cut(s)	477, 1442
BsgI	GTGCAG	1 cut(s)	1146
Bsh1236I	CGCG	2 cut(s)	420, 599
BshFI	GGCC	3 cut(s)	135, 596, 1021
BsiHKAI	GWGCWC	4 cut(s)	477, 1092, 1442, 1491
BsiHKCI	CYCGRG	1 cut(s)	1388
BsiSI	CCGG	6 cut(s)	26, 38, 266, 1018, 1025, 1431
BslFI	GGGAC	3 cut(s)	245, 1046, 1533
BslI	CCNNNNNNNGG	3 cut(s)	377, 682, 1024
BsmAI	GTCTC	6 cut(s)	198, 339, 534, 880, 1114, 1392
BsmBI	CGTCTC	3 cut(s)	198, 339, 1392
BsmFI	GGGAC	3 cut(s)	245, 1046, 1533
BsmI	GAATGC	4 cut(s)	83, 170, 570, 1459
BsnI	GGCC	3 cut(s)	135, 596, 1021
Bso31I	GGTCTC	1 cut(s)	1114
BsoBI	CYCGRG	1 cut(s)	1388
Bsp1286I	GDGCHC	5 cut(s)	477, 1092, 1442, 1491, 1533
Bsp143I	GATC	3 cut(s)	252, 421, 894
BspACI	CCGC	5 cut(s)	597, 599, 638, 922, 1474
BspANI	GGCC	3 cut(s)	135, 596, 1021
BspCNI	CTCAG	2 cut(s)	868, 1474
BspFNI	CGCG	2 cut(s)	420, 599
BspLI	GGNNCC	5 cut(s)	276, 815, 969, 1235, 1530
BspMAI	CTGCAG	1 cut(s)	388
BspMI	ACCTGC	1 cut(s)	377
BspPI	GGATC	3 cut(s)	247, 416, 902
BspTI	CTTAAG	1 cut(s)	1154
BspTNI	GGTCTC	1 cut(s)	1114
BsrDI	GCAATG	1 cut(s)	1194
BsrFI	RCCGGY	2 cut(s)	37, 1017
BsrI	ACTGG	9 cut(s)	150, 257, 478, 937, 977, 1170, 1333, 1496, 1551
BssAI	RCCGGY	2 cut(s)	37, 1017
BssECI	CCNNGG	3 cut(s)	597, 676, 890
BssMI	GATC	3 cut(s)	252, 421, 894
BssSI	CACGAG	1 cut(s)	560
BssT1I	CCWWGG	2 cut(s)	676, 890
Bst2BI	CACGAG	1 cut(s)	560
Bst4CI	ACNGT	3 cut(s)	1357, 1437, 1567
Bst6I	CTCTTC	5 cut(s)	201, 342, 534, 771, 1232
BstAFI	CTTAAG	1 cut(s)	1154
BstAPI	GCANNNNNTGC	2 cut(s)	392, 1471
BstC8I	GCNNGC	1 cut(s)	924
BstDEI	CTNAG	4 cut(s)	584, 855, 1203, 1482
BstDSI	CCRYGG	1 cut(s)	597
BstF5I	GGATG	2 cut(s)	1375, 1482
BstFNI	CGCG	2 cut(s)	420, 599
BstHHI	GCGC	2 cut(s)	739, 1459
BstKTI	GATC	3 cut(s)	255, 424, 897
BstMAI	GTCTC	6 cut(s)	198, 339, 534, 880, 1114, 1392
BstMBI	GATC	3 cut(s)	252, 421, 894
BstMWI	GCNNNNNNNGC	6 cut(s)	392, 1283, 1286, 1289, 1471, 1486
BstNSI	RCATGY	2 cut(s)	301, 951
BstSFI	CTRYAG	1 cut(s)	384
BstSLI	GKGCMC	2 cut(s)	477, 1442
BstUI	CGCG	2 cut(s)	420, 599
BstX2I	RGATCY	1 cut(s)	252
BstYI	RGATCY	1 cut(s)	252
Bsu36I	CCTNAGG	1 cut(s)	1482
BsuRI	GGCC	3 cut(s)	135, 596, 1021
BtgI	CCRYGG	1 cut(s)	597
BtsCI	GGATG	2 cut(s)	1375, 1482
BtsI	GCAGTG	2 cut(s)	63, 1112
BtsIMutI	CAGTG	8 cut(s)	63, 512, 1112, 1163, 1362, 1442, 1489, 1572
BveI	ACCTGC	1 cut(s)	377
Cac8I	GCNNGC	1 cut(s)	924
CfoI	GCGC	2 cut(s)	739, 1459
Cfr10I	RCCGGY	2 cut(s)	37, 1017
Cfr13I	GGNCC	2 cut(s)	134, 1222
Cfr42I	CCGCGG	1 cut(s)	600
CseI	GACGC	1 cut(s)	516
Csp6I	GTAC	1 cut(s)	143
CviAII	CATG	8 cut(s)	298, 351, 544, 592, 948, 1079, 1249, 1524
CviQI	GTAC	1 cut(s)	143
DdeI	CTNAG	4 cut(s)	584, 855, 1203, 1482
DpnI	GATC	3 cut(s)	254, 423, 896
DpnII	GATC	3 cut(s)	252, 421, 894
EaeI	YGGCCR	1 cut(s)	594
Eam1104I	CTCTTC	5 cut(s)	201, 342, 534, 771, 1232
EarI	CTCTTC	5 cut(s)	201, 342, 534, 771, 1232
Eco130I	CCWWGG	2 cut(s)	676, 890
Eco24I	GRGCYC	1 cut(s)	1533
Eco31I	GGTCTC	1 cut(s)	1114
Eco47I	GGWCC	1 cut(s)	1222
Eco57I	CTGAAG	4 cut(s)	536, 674, 1213, 1481
Eco81I	CCTNAGG	1 cut(s)	1482
Eco88I	CYCGRG	1 cut(s)	1388
EcoRI	GAATTC	1 cut(s)	1097
EcoT14I	CCWWGG	2 cut(s)	676, 890
EcoT22I	ATGCAT	3 cut(s)	83, 170, 311
EcoT38I	GRGCYC	1 cut(s)	1533
ErhI	CCWWGG	2 cut(s)	676, 890
Esp3I	CGTCTC	3 cut(s)	198, 339, 1392
FaeI	CATG	8 cut(s)	301, 354, 547, 595, 951, 1082, 1252, 1527
FalI	AAGNNNNNCTT	2 cut(s)	1196, 1228
FaqI	GGGAC	3 cut(s)	245, 1046, 1533
FatI	CATG	8 cut(s)	297, 350, 543, 591, 947, 1078, 1248, 1523
FauI	CCCGC	1 cut(s)	915
FblI	GTMKAC	1 cut(s)	415
FokI	GGATG	2 cut(s)	1362, 1489
FriOI	GRGCYC	1 cut(s)	1533
FspBI	CTAG	1 cut(s)	1448
FspI	TGCGCA	1 cut(s)	738
GlaI	GCGC	2 cut(s)	738, 1458
GsuI	CTGGAG	2 cut(s)	167, 954
HaeIII	GGCC	3 cut(s)	135, 596, 1021
HapII	CCGG	6 cut(s)	26, 38, 266, 1018, 1025, 1431
HgaI	GACGC	1 cut(s)	516
HhaI	GCGC	2 cut(s)	739, 1459
Hin1II	CATG	8 cut(s)	301, 354, 547, 595, 951, 1082, 1252, 1527
Hin6I	GCGC	2 cut(s)	737, 1457
HinP1I	GCGC	2 cut(s)	737, 1457
HindIII	AAGCTT	1 cut(s)	1172
HpaII	CCGG	6 cut(s)	26, 38, 266, 1018, 1025, 1431
HphI	GGTGA	3 cut(s)	99, 644, 806
Hpy166II	GTNNAC	5 cut(s)	416, 475, 553, 1360, 1440
Hpy188I	TCNGA	5 cut(s)	405, 460, 576, 654, 1245
Hpy188III	TCNNGA	9 cut(s)	243, 287, 425, 533, 554, 823, 857, 1000, 1390
Hpy8I	GTNNAC	5 cut(s)	416, 475, 553, 1360, 1440
Hpy99I	CGWCG	1 cut(s)	510
HpyAV	CCTTC	7 cut(s)	265, 571, 623, 824, 901, 955, 1124
HpyCH4III	ACNGT	3 cut(s)	1357, 1437, 1567
HpyCH4IV	ACGT	2 cut(s)	649, 1539
HpyF10VI	GCNNNNNNNGC	6 cut(s)	392, 1283, 1286, 1289, 1471, 1486
HpyF3I	CTNAG	4 cut(s)	584, 855, 1203, 1482
HpySE526I	ACGT	2 cut(s)	649, 1539
Hsp92II	CATG	8 cut(s)	301, 354, 547, 595, 951, 1082, 1252, 1527
HspAI	GCGC	2 cut(s)	737, 1457
KspI	CCGCGG	1 cut(s)	600
Kzo9I	GATC	3 cut(s)	252, 421, 894
LmnI	GCTCC	7 cut(s)	274, 461, 580, 685, 1095, 1486, 1528
LweI	GCATC	3 cut(s)	724, 1048, 1467
MaeI	CTAG	1 cut(s)	1448
MaeII	ACGT	2 cut(s)	649, 1539
MaeIII	GTNAC	2 cut(s)	105, 1013
MalI	GATC	3 cut(s)	254, 423, 896
MboI	GATC	3 cut(s)	252, 421, 894
MfeI	CAATTG	1 cut(s)	991
MflI	RGATCY	1 cut(s)	252
MhlI	GDGCHC	5 cut(s)	477, 1092, 1442, 1491, 1533
MluCI	AATT	6 cut(s)	843, 981, 991, 1097, 1326, 1469
MlyI	GAGTC	2 cut(s)	1028, 1218
MmeI	TCCRAC	1 cut(s)	1200
Mph1103I	ATGCAT	3 cut(s)	83, 170, 311
MroXI	GAANNNNTTC	1 cut(s)	818
MseI	TTAA	2 cut(s)	846, 1155
MslI	CAYNNNNRTG	1 cut(s)	1494
MspA1I	CMGCKG	1 cut(s)	599
MspCI	CTTAAG	1 cut(s)	1154
MspI	CCGG	6 cut(s)	26, 38, 266, 1018, 1025, 1431
MunI	CAATTG	1 cut(s)	991
Mva1269I	GAATGC	4 cut(s)	83, 170, 570, 1459
MvnI	CGCG	2 cut(s)	420, 599
MwoI	GCNNNNNNNGC	6 cut(s)	392, 1283, 1286, 1289, 1471, 1486
NdeII	GATC	3 cut(s)	252, 421, 894
NlaIII	CATG	8 cut(s)	301, 354, 547, 595, 951, 1082, 1252, 1527
NlaIV	GGNNCC	5 cut(s)	276, 815, 969, 1235, 1530
NmuCI	GTSAC	1 cut(s)	105
NsbI	TGCGCA	1 cut(s)	738
NsiI	ATGCAT	3 cut(s)	83, 170, 311
NspI	RCATGY	2 cut(s)	301, 951
PctI	GAATGC	4 cut(s)	83, 170, 570, 1459
PdmI	GAANNNNTTC	1 cut(s)	818
PfeI	GAWTC	9 cut(s)	7, 19, 115, 239, 292, 819, 886, 902, 1006
PflMI	CCANNNNNTGG	1 cut(s)	682
PleI	GAGTC	2 cut(s)	1028, 1217
PpsI	GAGTC	2 cut(s)	1028, 1217
PspN4I	GGNNCC	5 cut(s)	276, 815, 969, 1235, 1530
PspPI	GGNCC	2 cut(s)	134, 1222
PstI	CTGCAG	1 cut(s)	388
PsuI	RGATCY	1 cut(s)	252
RsaI	GTAC	1 cut(s)	144
RsaNI	GTAC	1 cut(s)	143
RseI	CAYNNNNRTG	1 cut(s)	1494
SacII	CCGCGG	1 cut(s)	600
SaqAI	TTAA	2 cut(s)	846, 1155
Sau3AI	GATC	3 cut(s)	252, 421, 894
Sau96I	GGNCC	2 cut(s)	134, 1222
ScaI	AGTACT	1 cut(s)	144
SchI	GAGTC	2 cut(s)	1028, 1218
SduI	GDGCHC	5 cut(s)	477, 1092, 1442, 1491, 1533
SfaNI	GCATC	3 cut(s)	724, 1048, 1467
SfcI	CTRYAG	1 cut(s)	384
Sfr303I	CCGCGG	1 cut(s)	600
SgrAI	CRCCGGYG	1 cut(s)	37
SgrBI	CCGCGG	1 cut(s)	600
SinI	GGWCC	1 cut(s)	1222
SmiMI	CAYNNNNRTG	1 cut(s)	1494
SmlI	CTYRAG	2 cut(s)	533, 1154
SmoI	CTYRAG	2 cut(s)	533, 1154
SpeI	ACTAGT	1 cut(s)	1447
Sse9I	AATT	6 cut(s)	843, 981, 991, 1097, 1326, 1469
SsiI	CCGC	5 cut(s)	597, 599, 638, 922, 1474
SspMI	CTAG	1 cut(s)	1448
StyI	CCWWGG	2 cut(s)	676, 890
TaaI	ACNGT	3 cut(s)	1357, 1437, 1567
TaiI	ACGT	2 cut(s)	652, 1542
TaqI	TCGA	2 cut(s)	905, 1095
TasI	AATT	6 cut(s)	843, 981, 991, 1097, 1326, 1469
TatI	WGTACW	1 cut(s)	142
TauI	GCSGC	1 cut(s)	599
TfiI	GAWTC	9 cut(s)	7, 19, 115, 239, 292, 819, 886, 902, 1006
Tru1I	TTAA	2 cut(s)	846, 1155
Tru9I	TTAA	2 cut(s)	846, 1155
TscAI	CASTG	8 cut(s)	63, 519, 1119, 1170, 1362, 1442, 1496, 1572
TseFI	GTSAC	1 cut(s)	105
Tsp45I	GTSAC	1 cut(s)	105
TspDTI	ATGAA	4 cut(s)	500, 843, 1154, 1529
TspGWI	ACGGA	1 cut(s)	1361
TspRI	CASTG	8 cut(s)	63, 519, 1119, 1170, 1362, 1442, 1496, 1572
Van91I	CCANNNNNTGG	1 cut(s)	682
Vha464I	CTTAAG	1 cut(s)	1154
VneI	GTGCAC	2 cut(s)	473, 1438
VpaK11BI	GGWCC	1 cut(s)	1222
XapI	RAATTY	1 cut(s)	1097
XceI	RCATGY	2 cut(s)	301, 951
XmiI	GTMKAC	1 cut(s)	415
XmnI	GAANNNNTTC	1 cut(s)	818
XspI	CTAG	1 cut(s)	1448
ZrmI	AGTACT	1 cut(s)	144
Zsp2I	ATGCAT	3 cut(s)	83, 170, 311

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.