FvH4_3g18270

Polynucleotide kinase 3 phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005030
Physical Location & Seq
Reverse (-)
11682575 .. 11688270
5696 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g18270.t2

Sequence Viewer

Length: 1455 bp
ATGCTACCATTGTTCACTTTCCACGTCTCTAAATCCATCATCATCTCTTCACTAATAATCCCCACCTCCCCAAAACCCCTATTTCCCAATCTCTCTCCGATCCGTTTCTTCCAAACCCCGAAACATTTCGTCTGCATTCGCTCCCAGAAACCCTCAATGTCGTCTTCTTCTCCGACCCATGTGGAAGCCCAGTACGCCAAGTCCGGCAATTCGTCGTGCAAGAAGTGCTCGGAGCCGATTGAGAACCAAGCTCTGAGGCTGGGCACCAAAGACGCGCGTGGCTATACTAAGTGGCACCACTTGGGTTGCTTCAGTTTTGGGTCCGAGTCTGTTGCTTCGGTTGACATGATTAAAGGGTTTCAGTCTCTCAAGAGTAGTGATCAGGAAGCTTTAAAGGATGACCTTGAGAAATCTAAAGAGGAGGATTCAAATGGAAAGAAAAGCAGTCAAAATCGAAAGGTTCGTGAAGTAAAAGAGGCTGAAGATGAAAATGTAGGGGAGAGGGAGTTGAAGAAACCAAAGGGTTCTTCTTCTGCTGTGATCCATGTTGAAGCCGAGTACGCCAAGTCCAGCAGGTCCTCATGCAAGAAGTGCTTGGAGCCGATCGAGAAACAAGCTCTGAGATTGGGCACCAAAGACACCCGTGGTTATACCATGTGGCACCACTTGGGTTGCTTCTCTTTTGGGTCCGAGTCTGTTGCTTCGGTTGACAAGATTAAAGGGTTTCAGTCTCTGAAGCATGGTGATCAGGAAGCTTTGAAGAATTTGGCAGATGAATTTGAGAAATCTAAAGATGAGGATTCAAATGGAGAGAAAAGTTCTGAAAATCAAAAGGTATCCAACTTGGAAATATCCTTTTCAGTGTCTGATGTGAAGGACAAGTACAAGGATGCAACTCTTCCGCCTAAATGGAAGGCATTTCAGACTATCATATATCTCGAAAGGGATGATGGTCTGCATGATTCAAGTAAAATTGCAGCTTTTGATTTTGATGGATGTCTTGCAAATACTGATGTTAGGAGAGTAGGTGCCGATGCTTGGTCCCTAATGTATCCTTCAATACCTGATAAGCTACGGAGCTTGTACAAGGATGGCTACAAGCTGGTGATCTTCACTAATGAAGCCAACATTGAGCGCTGGAAAAATAAAAGACAAGTAGCTGTGGACTCAAAAATTGGACGTCTCAACGATTTTATCAAGGAAGTGAATGTCCCAATTCAGGTTTTCATAGCCTGTGGGCCGAGTCGAGGAGAAGATCCCTTTCGGAAACCCAAACCGGGGATGTGGCATATTTTGGAACAACACTTCAACTCTGGCATTTCCATCGATATGGATCAATCTTTTTATGTTGGTGATGCTGCTGGAAGAAGCAATGATCACAGTGATGCTGATCTCAAGTTTGCAGAGGCCAATGGTTTGAAGTTTTATGTCCCGGAGGATTACTTTGGAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

485

Amino Acids

54.29

Weight (kDa)

7.94

Isoelectric Point (pI)

45.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-PARP PF00645 64 - 133 3.3e-15 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
zf-PARP PF00645 187 - 256 2.9e-16 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PNK3P PF08645 325 - 483 1.5e-46 Polynucleotide kinase 3 phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1183
Acc36I ACCTGC 1 cut(s) 564
AccB1I GGYRCC 5 cut(s) 263, 294, 629, 660, 1028
AccII CGCG 2 cut(s) 275, 277
AciI CCGC 1 cut(s) 902
AclWI GGATC 4 cut(s) 94, 535, 1250, 1341
AcsI RAATTY 2 cut(s) 763, 776
AcuI CTGAAG 3 cut(s) 295, 501, 755
AcyI GRCGYC 1 cut(s) 1180
AfaI GTAC 4 cut(s) 194, 560, 884, 1085
AfeI AGCGCT 1 cut(s) 1136
AfiI CCNNNNNNNGG 5 cut(s) 1038, 1219, 1247, 1277, 1278
AgsI TTSAA 9 cut(s) 429, 511, 551, 760, 804, 966, 1059, 1309, 1420
AjiI CACGTC 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 511, 543
AluBI AGCT 9 cut(s) 251, 389, 617, 755, 980, 1072, 1080, 1102, 1160
AluI AGCT 9 cut(s) 251, 389, 617, 755, 980, 1072, 1080, 1102, 1160
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 4 cut(s) 31, 369, 735, 1187
AlwI GGATC 4 cut(s) 94, 535, 1250, 1341
AlwNI CAGNNNCTG 2 cut(s) 733, 866
Aor51HI AGCGCT 1 cut(s) 1136
AoxI GGCC 2 cut(s) 1238, 1407
ApeKI GCWGC 2 cut(s) 977, 1358
ApoI RAATTY 2 cut(s) 763, 776
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 2 cut(s) 277, 1137
AspS9I GGNCC 5 cut(s) 321, 576, 687, 1041, 1238
AsuC2I CCSGG 2 cut(s) 1278, 1433
AsuHPI GGTGA 3 cut(s) 755, 1117, 1364
AvaII GGWCC 4 cut(s) 321, 576, 687, 1041
BaeGI GKGCMC 2 cut(s) 266, 632
BanI GGYRCC 5 cut(s) 263, 294, 629, 660, 1028
BarI GAAGNNNNNNTAC 2 cut(s) 866, 898
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 230
BbvI GCAGC 2 cut(s) 989, 1345
BccI CCATC 5 cut(s) 44, 944, 986, 1085, 1331
BcgI CGANNNNNNTGC 6 cut(s) 314, 348, 680, 714, 1306, 1340
BciVI GTATCC 2 cut(s) 847, 1062
BclI TGATCA 3 cut(s) 379, 745, 1375
BcnI CCSGG 2 cut(s) 1278, 1433
BcoDI GTCTC 4 cut(s) 31, 369, 735, 1187
BfoI RGCGCY 1 cut(s) 1138
BfuAI ACCTGC 1 cut(s) 564
BfuI GTATCC 2 cut(s) 847, 1062
BisI GCNGC 2 cut(s) 978, 1359
BlsI GCNGC 2 cut(s) 979, 1360
Bme1390I CCNGG 2 cut(s) 1278, 1433
Bme18I GGWCC 4 cut(s) 321, 576, 687, 1041
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 5 cut(s) 321, 576, 687, 1041, 1238
BmrFI CCNGG 2 cut(s) 1278, 1433
BmrI ACTGGG 1 cut(s) 184
BmsI GCATC 4 cut(s) 880, 1024, 1345, 1375
BmuI ACTGGG 1 cut(s) 184
BpiI GAAGAC 1 cut(s) 156
BpuEI CTTGAG 3 cut(s) 353, 425, 1379
BpuMI CCSGG 2 cut(s) 1278, 1433
Bsa29I ATCGAT 1 cut(s) 1326
BsaBI GATNNNNATC 2 cut(s) 1332, 1389
BsaHI GRCGYC 1 cut(s) 1180
BsaJI CCNNGG 2 cut(s) 643, 1277
BsaXI ACNNNNNCTCC 4 cut(s) 491, 521, 1012, 1042
Bsc4I CCNNNNNNNGG 5 cut(s) 1038, 1219, 1247, 1277, 1278
Bse1I ACTGG 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 1378
Bse8I GATNNNNATC 2 cut(s) 1332, 1389
BseCI ATCGAT 1 cut(s) 1326
BseDI CCNNGG 2 cut(s) 643, 1277
BseGI GGATG 6 cut(s) 403, 895, 952, 1001, 1096, 1287
BseJI GATNNNNATC 2 cut(s) 1332, 1389
BseLI CCNNNNNNNGG 5 cut(s) 1038, 1219, 1247, 1277, 1278
BseMI GCAATG 1 cut(s) 1378
BseMII CTCAG 2 cut(s) 245, 611
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 2 cut(s) 434, 1263
BseSI GKGCMC 2 cut(s) 266, 632
BseXI GCAGC 2 cut(s) 989, 1345
BseYI CCCAGC 1 cut(s) 259
Bsh1236I CGCG 2 cut(s) 275, 277
Bsh1285I CGRYCG 1 cut(s) 606
BshFI GGCC 2 cut(s) 1240, 1409
BshNI GGYRCC 5 cut(s) 263, 294, 629, 660, 1028
BshVI ATCGAT 1 cut(s) 1326
BsiEI CGRYCG 1 cut(s) 606
BsiHKAI GWGCWC 1 cut(s) 230
BsiSI CCGG 3 cut(s) 204, 1277, 1433
BslFI GGGAC 3 cut(s) 1027, 1196, 1415
BslI CCNNNNNNNGG 5 cut(s) 1038, 1219, 1247, 1277, 1278
BsmAI GTCTC 4 cut(s) 31, 369, 735, 1187
BsmBI CGTCTC 2 cut(s) 31, 1187
BsmFI GGGAC 3 cut(s) 1027, 1196, 1415
BsmI GAATGC 1 cut(s) 135
BsnI GGCC 2 cut(s) 1240, 1409
Bsp1286I GDGCHC 3 cut(s) 230, 266, 632
Bsp1407I TGTACA 1 cut(s) 1083
BspACI CCGC 1 cut(s) 902
BspANI GGCC 2 cut(s) 1240, 1409
BspCNI CTCAG 2 cut(s) 246, 612
BspDI ATCGAT 1 cut(s) 1326
BspFNI CGCG 2 cut(s) 275, 277
BspMI ACCTGC 1 cut(s) 564
BspPI GGATC 4 cut(s) 94, 535, 1250, 1341
BspT107I GGYRCC 5 cut(s) 263, 294, 629, 660, 1028
BsrDI GCAATG 1 cut(s) 1378
BsrGI TGTACA 1 cut(s) 1083
BsrI ACTGG 1 cut(s) 190
BssECI CCNNGG 2 cut(s) 643, 1277
BssNI GRCGYC 1 cut(s) 1180
Bst4CI ACNGT 1 cut(s) 1382
Bst6I CTCTTC 2 cut(s) 52, 903
BstACI GRCGYC 1 cut(s) 1180
BstAPI GCANNNNNTGC 2 cut(s) 225, 591
BstAUI TGTACA 1 cut(s) 1083
BstDEI CTNAG 3 cut(s) 254, 288, 620
BstDSI CCRYGG 1 cut(s) 643
BstF5I GGATG 6 cut(s) 403, 895, 952, 1001, 1096, 1287
BstFNI CGCG 2 cut(s) 275, 277
BstH2I RGCGCY 1 cut(s) 1138
BstHHI GCGC 2 cut(s) 277, 1137
BstMAI GTCTC 4 cut(s) 31, 369, 735, 1187
BstMCI CGRYCG 1 cut(s) 606
BstMWI GCNNNNNNNGC 4 cut(s) 194, 225, 560, 591
BstSCI CCNGG 2 cut(s) 1276, 1431
BstSLI GKGCMC 2 cut(s) 266, 632
BstUI CGCG 2 cut(s) 275, 277
BstV1I GCAGC 2 cut(s) 989, 1345
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 1 cut(s) 1255
BstXI CCANNNNNNTGG 1 cut(s) 1330
BstYI RGATCY 1 cut(s) 1255
Bsu15I ATCGAT 1 cut(s) 1326
BsuI GTATCC 2 cut(s) 847, 1062
BsuRI GGCC 2 cut(s) 1240, 1409
BsuTUI ATCGAT 1 cut(s) 1326
BtgI CCRYGG 1 cut(s) 643
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 6 cut(s) 403, 895, 952, 1001, 1096, 1287
BtsIMutI CAGTG 2 cut(s) 867, 1387
BveI ACCTGC 1 cut(s) 564
CaiI CAGNNNCTG 2 cut(s) 733, 866
CfoI GCGC 2 cut(s) 277, 1137
Cfr13I GGNCC 5 cut(s) 321, 576, 687, 1041, 1238
ClaI ATCGAT 1 cut(s) 1326
CseI GACGC 1 cut(s) 281
Csp6I GTAC 4 cut(s) 193, 559, 883, 1084
CspCI CAANNNNNGTGG 4 cut(s) 287, 322, 653, 688
CviAII CATG 7 cut(s) 179, 346, 545, 582, 655, 740, 959
CviQI GTAC 4 cut(s) 193, 559, 883, 1084
DdeI CTNAG 3 cut(s) 254, 288, 620
DraI TTTAAA 1 cut(s) 393
Eam1104I CTCTTC 2 cut(s) 52, 903
EarI CTCTTC 2 cut(s) 52, 903
EciI GGCGGA 1 cut(s) 891
Eco47I GGWCC 4 cut(s) 321, 576, 687, 1041
Eco47III AGCGCT 1 cut(s) 1136
Eco57I CTGAAG 3 cut(s) 295, 501, 755
EcoO109I RGGNCCY 1 cut(s) 576
Esp3I CGTCTC 2 cut(s) 31, 1187
FaeI CATG 7 cut(s) 182, 349, 548, 585, 658, 743, 962
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 3 cut(s) 1027, 1196, 1415
FatI CATG 7 cut(s) 178, 345, 544, 581, 654, 739, 958
FbaI TGATCA 3 cut(s) 379, 745, 1375
Fnu4HI GCNGC 2 cut(s) 978, 1359
FokI GGATG 6 cut(s) 410, 902, 959, 1008, 1103, 1294
Fsp4HI GCNGC 2 cut(s) 978, 1359
GlaI GCGC 2 cut(s) 276, 1136
GluI GCNGC 2 cut(s) 978, 1359
GsaI CCCAGC 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 1138
HaeIII GGCC 2 cut(s) 1240, 1409
HapII CCGG 3 cut(s) 204, 1277, 1433
HgaI GACGC 1 cut(s) 281
HhaI GCGC 2 cut(s) 277, 1137
Hin1I GRCGYC 1 cut(s) 1180
Hin1II CATG 7 cut(s) 182, 349, 548, 585, 658, 743, 962
Hin6I GCGC 2 cut(s) 275, 1135
HinP1I GCGC 2 cut(s) 275, 1135
HincII GTYRAC 2 cut(s) 343, 709
HindII GTYRAC 2 cut(s) 343, 709
HindIII AAGCTT 2 cut(s) 387, 753
HinfI GANTC 7 cut(s) 326, 425, 692, 800, 962, 1166, 1243
HpaII CCGG 3 cut(s) 204, 1277, 1433
HphI GGTGA 3 cut(s) 755, 1117, 1364
Hpy166II GTNNAC 4 cut(s) 15, 343, 709, 1165
Hpy188III TCNNGA 6 cut(s) 370, 383, 464, 607, 749, 938
Hpy8I GTNNAC 4 cut(s) 15, 343, 709, 1165
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 3 cut(s) 868, 907, 1065
HpyCH4III ACNGT 1 cut(s) 1382
HpyCH4IV ACGT 2 cut(s) 24, 1180
HpyCH4V TGCA 8 cut(s) 135, 219, 585, 893, 958, 977, 1004, 1403
HpyF10VI GCNNNNNNNGC 4 cut(s) 194, 225, 560, 591
HpyF3I CTNAG 3 cut(s) 254, 288, 620
HpySE526I ACGT 2 cut(s) 24, 1180
Hsp92I GRCGYC 1 cut(s) 1180
Hsp92II CATG 7 cut(s) 182, 349, 548, 585, 658, 743, 962
HspAI GCGC 2 cut(s) 275, 1135
Ksp22I TGATCA 3 cut(s) 379, 745, 1375
LmnI GCTCC 4 cut(s) 146, 232, 598, 1077
Lsp1109I GCAGC 2 cut(s) 989, 1345
LweI GCATC 4 cut(s) 880, 1024, 1345, 1375
MaeII ACGT 2 cut(s) 24, 1180
MflI RGATCY 1 cut(s) 1255
MhlI GDGCHC 3 cut(s) 230, 266, 632
MluCI AATT 6 cut(s) 208, 763, 776, 972, 1173, 1215
MlyI GAGTC 4 cut(s) 335, 701, 1160, 1252
MmeI TCCRAC 2 cut(s) 197, 864
MroXI GAANNNNTTC 1 cut(s) 125
MseI TTAA 3 cut(s) 351, 392, 717
MslI CAYNNNNRTG 2 cut(s) 1328, 1383
MspI CCGG 3 cut(s) 204, 1277, 1433
MspR9I CCNGG 2 cut(s) 1278, 1433
Mva1269I GAATGC 1 cut(s) 135
MvnI CGCG 2 cut(s) 275, 277
MwoI GCNNNNNNNGC 4 cut(s) 194, 225, 560, 591
NciI CCSGG 2 cut(s) 1278, 1433
NlaIII CATG 7 cut(s) 182, 349, 548, 585, 658, 743, 962
NmeAIII GCCGAG 2 cut(s) 580, 1266
PcsI WCGNNNNNNNCGW 1 cut(s) 460
PctI GAATGC 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 3 cut(s) 425, 800, 962
PfoI TCCNGGA 1 cut(s) 1431
PkrI GCNGC 2 cut(s) 979, 1360
Ple19I CGATCG 1 cut(s) 606
PleI GAGTC 4 cut(s) 334, 700, 1160, 1251
PpsI GAGTC 4 cut(s) 334, 700, 1160, 1251
PpuMI RGGWCCY 1 cut(s) 576
Psp5II RGGWCCY 1 cut(s) 576
PspFI CCCAGC 1 cut(s) 259
PspPI GGNCC 5 cut(s) 321, 576, 687, 1041, 1238
PspPPI RGGWCCY 1 cut(s) 576
PstNI CAGNNNCTG 2 cut(s) 733, 866
PsuI RGATCY 1 cut(s) 1255
PvuI CGATCG 1 cut(s) 606
RsaI GTAC 4 cut(s) 194, 560, 884, 1085
RsaNI GTAC 4 cut(s) 193, 559, 883, 1084
RseI CAYNNNNRTG 2 cut(s) 1328, 1383
SaqAI TTAA 3 cut(s) 351, 392, 717
SatI GCNGC 2 cut(s) 978, 1359
Sau96I GGNCC 5 cut(s) 321, 576, 687, 1041, 1238
SchI GAGTC 4 cut(s) 335, 701, 1160, 1252
ScrFI CCNGG 2 cut(s) 1278, 1433
SduI GDGCHC 3 cut(s) 230, 266, 632
SfaNI GCATC 4 cut(s) 880, 1024, 1345, 1375
SinI GGWCC 4 cut(s) 321, 576, 687, 1041
SmiMI CAYNNNNRTG 2 cut(s) 1328, 1383
SmlI CTYRAG 3 cut(s) 368, 404, 1394
SmoI CTYRAG 3 cut(s) 368, 404, 1394
Sse9I AATT 6 cut(s) 208, 763, 776, 972, 1173, 1215
SsiI CCGC 1 cut(s) 902
StyD4I CCNGG 2 cut(s) 1276, 1431
TaaI ACNGT 1 cut(s) 1382
TaiI ACGT 2 cut(s) 27, 1183
TaqI TCGA 5 cut(s) 454, 606, 939, 1246, 1326
TasI AATT 6 cut(s) 208, 763, 776, 972, 1173, 1215
TatI WGTACW 2 cut(s) 882, 1083
TfiI GAWTC 3 cut(s) 425, 800, 962
Tru1I TTAA 3 cut(s) 351, 392, 717
Tru9I TTAA 3 cut(s) 351, 392, 717
TscAI CASTG 2 cut(s) 867, 1387
TseI GCWGC 2 cut(s) 977, 1358
TspDTI ATGAA 4 cut(s) 501, 789, 1134, 1216
TspGWI ACGGA 2 cut(s) 92, 1090
TspRI CASTG 2 cut(s) 867, 1387
VpaK11BI GGWCC 4 cut(s) 321, 576, 687, 1041
XapI RAATTY 2 cut(s) 763, 776
XmnI GAANNNNTTC 1 cut(s) 125
ZraI GACGTC 1 cut(s) 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.