RLG00000033264

Polynucleotide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005030
Physical Location & Seq
Reverse (-)
25079180 .. 25083539
4360 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033264

Sequence Viewer

Length: 1152 bp
ATGCTACCCATCGCAATTGTCTGTAAATCGATCATCTCTTGCCTAATCATCCCCGCCTTCCCAAAACCCCTAATTCCGAGTCACTCTCCGAAACGTTTCTTCCAAACCCCGAAACGTTTCGCCTGTATTCGCTCCCAAAAACCACCAATGTCTTCTTCTTCTACGACCCATGTCACCGCCGAGTACGCCAAGTCCAACAGGTCCTCGTGCAAGAAATGCTCGAAAAAGATCGAGGCCAAAGCTCTGAGGCTGGGCTTGGTCACCAGAGACGCGCGTGGTTTCGACGCCACCAAGTGGCACCACTTGGGTTGCTTCAGTTTTGGGTCCGAGTTGGTTGCTTCGGTTGACAAGATTAAAGGTTTTCAGTCTCTCAAGAGCAGTGATCAGGAAGCTTTGAAGAATTTGGTGGATGAATTTGAGAAATCCAAAGAGGAGGATTCAAATGGAAAGAAAGGTTCTCAAAAACAAAAGGTTTGTGAAGTAGATGAAGATGAAAATGAAGTAGGGGAAAGGGAGTTGAAGAAAGCGAAGGTATCTAACCTGGAAATATCCTTTTCAGTGTCTGATGTGAAGGACAAGTACAAGGAAGCAACTCTTCCACCTAAATGGAAGGCATTTCAGACTATCATATTTCTTGAAAGGGATGATGGTCTGCATGATTCAAGTAAAATTGCTGCTTTTGATTTTGATGGATGTCTTGCAAATACTGATGTTAGGAGAGTCGGTGCTGATGCTTGGTCCCTAATGTATCCTTCAATACCTGATAAACTACGGAGCTTGTATGAGGATGGCTACAAGCTGGTAATCTTCACGAATGAAGCCAACATTGAGCGCTGGAAAAATAAGAGACAAGTAGCTGTGGACTCAAAAATTGGACGTCTCAACGATTTTATTAAGGAAGTGAAGGTCCCAATTCAGGTTTTCATAGCTTGTGGGACGAGTCAAGGAGAAGACCCCTTTCGCAAACCCAAACCGGGGTTGTGGCATATGTTGGAACAACACTTCAACTCTCGCATTTCCATTGATATGAATCAATCCTTTTATGTTGGTGATGCTGCTGGAAGAAGCAATGATCACAGTGATGCTGATCTTAAGTTTGCAGAGGCCAATGGTTTAAAGTTTTATGTCCCAGAGGATTTCTTTGAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

384

Amino Acids

43.21

Weight (kDa)

8.67

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-PARP PF00645 61 - 135 9.9e-18 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PNK3P PF08645 223 - 381 3.1e-46 Polynucleotide kinase 3 phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 880
AccB1I GGYRCC 1 cut(s) 297
AccB7I CCANNNNNTGG 1 cut(s) 294
AccII CGCG 2 cut(s) 272, 274
AciI CCGC 2 cut(s) 54, 177
AclI AACGTT 2 cut(s) 94, 115
AcsI RAATTY 2 cut(s) 400, 413
AcuI CTGAAG 1 cut(s) 298
AcyI GRCGYC 2 cut(s) 285, 877
AdeI CACNNNGTG 1 cut(s) 294
AfaI GTAC 2 cut(s) 185, 581
AfeI AGCGCT 1 cut(s) 833
AfiI CCNNNNNNNGG 4 cut(s) 294, 916, 974, 975
AflII CTTAAG 1 cut(s) 1091
AgsI TTSAA 8 cut(s) 397, 441, 520, 638, 663, 756, 1006, 1145
AjnI CCWGG 1 cut(s) 540
AjuI GAANNNNNNNTTGG 2 cut(s) 139, 171
AluBI AGCT 6 cut(s) 242, 392, 777, 799, 857, 929
AluI AGCT 6 cut(s) 242, 392, 777, 799, 857, 929
Alw26I GTCTC 4 cut(s) 261, 372, 841, 884
AlwNI CAGNNNCTG 1 cut(s) 563
Aor51HI AGCGCT 1 cut(s) 833
AoxI GGCC 2 cut(s) 234, 1104
ApeKI GCWGC 2 cut(s) 674, 1055
ApoI RAATTY 2 cut(s) 400, 413
Asp700I GAANNNNTTC 2 cut(s) 95, 116
AspLEI GCGC 2 cut(s) 274, 834
AspS9I GGNCC 4 cut(s) 201, 324, 738, 907
AsuC2I CCSGG 1 cut(s) 975
AsuHPI GGTGA 3 cut(s) 166, 253, 1061
AvaII GGWCC 4 cut(s) 201, 324, 738, 907
BanI GGYRCC 1 cut(s) 297
BarI GAAGNNNNNNTAC 2 cut(s) 563, 595
BauI CACGAG 1 cut(s) 205
BbsI GAAGAC 2 cut(s) 144, 957
BbvI GCAGC 2 cut(s) 661, 1042
BccI CCATC 4 cut(s) 17, 641, 683, 782
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 1 cut(s) 542
BciVI GTATCC 1 cut(s) 759
BclI TGATCA 2 cut(s) 382, 1072
BcnI CCSGG 1 cut(s) 975
BcoDI GTCTC 4 cut(s) 261, 372, 841, 884
BfoI RGCGCY 1 cut(s) 835
BfrI CTTAAG 1 cut(s) 1091
BfuI GTATCC 1 cut(s) 759
BisI GCNGC 2 cut(s) 675, 1056
BlsI GCNGC 2 cut(s) 676, 1057
Bme1390I CCNGG 2 cut(s) 542, 975
Bme18I GGWCC 4 cut(s) 201, 324, 738, 907
BmgT120I GGNCC 4 cut(s) 201, 324, 738, 907
BmiI GGNNCC 4 cut(s) 299, 325, 740, 909
BmrFI CCNGG 2 cut(s) 542, 975
BmsI GCATC 3 cut(s) 721, 1042, 1072
BoxI GACNNNNGTC 1 cut(s) 170
BpiI GAAGAC 2 cut(s) 144, 957
BplI GAGNNNNNCTC 2 cut(s) 70, 102
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 1 cut(s) 975
Bsa29I ATCGAT 1 cut(s) 29
BsaBI GATNNNNATC 2 cut(s) 1029, 1086
BsaHI GRCGYC 2 cut(s) 285, 877
BsaJI CCNNGG 1 cut(s) 974
BsaXI ACNNNNNCTCC 2 cut(s) 709, 739
Bsc4I CCNNNNNNNGG 4 cut(s) 294, 916, 974, 975
Bse3DI GCAATG 1 cut(s) 1075
Bse8I GATNNNNATC 2 cut(s) 1029, 1086
BseBI CCWGG 1 cut(s) 542
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 1 cut(s) 974
BseGI GGATG 5 cut(s) 48, 415, 649, 698, 793
BseJI GATNNNNATC 2 cut(s) 1029, 1086
BseLI CCNNNNNNNGG 4 cut(s) 294, 916, 974, 975
BseMI GCAATG 1 cut(s) 1075
BseMII CTCAG 1 cut(s) 236
BseRI GAGGAG 1 cut(s) 446
BseXI GCAGC 2 cut(s) 661, 1042
BseYI CCCAGC 1 cut(s) 250
Bsh1236I CGCG 2 cut(s) 272, 274
BshFI GGCC 2 cut(s) 236, 1106
BshNI GGYRCC 1 cut(s) 297
BshVI ATCGAT 1 cut(s) 29
BsiSI CCGG 1 cut(s) 974
BslFI GGGAC 4 cut(s) 724, 893, 949, 1112
BslI CCNNNNNNNGG 4 cut(s) 294, 916, 974, 975
BsmAI GTCTC 4 cut(s) 261, 372, 841, 884
BsmBI CGTCTC 2 cut(s) 261, 884
BsmFI GGGAC 4 cut(s) 724, 893, 949, 1112
BsnI GGCC 2 cut(s) 236, 1106
Bsp143I GATC 5 cut(s) 30, 228, 382, 1072, 1087
BspACI CCGC 2 cut(s) 54, 177
BspANI GGCC 2 cut(s) 236, 1106
BspCNI CTCAG 1 cut(s) 237
BspDI ATCGAT 1 cut(s) 29
BspFNI CGCG 2 cut(s) 272, 274
BspLI GGNNCC 4 cut(s) 299, 325, 740, 909
BspT107I GGYRCC 1 cut(s) 297
BspTI CTTAAG 1 cut(s) 1091
BsrDI GCAATG 1 cut(s) 1075
BssECI CCNNGG 1 cut(s) 974
BssMI GATC 5 cut(s) 30, 228, 382, 1072, 1087
BssNI GRCGYC 2 cut(s) 285, 877
BssSI CACGAG 1 cut(s) 205
Bst2BI CACGAG 1 cut(s) 205
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 1 cut(s) 1079
Bst6I CTCTTC 1 cut(s) 600
BstACI GRCGYC 2 cut(s) 285, 877
BstAFI CTTAAG 1 cut(s) 1091
BstAPI GCANNNNNTGC 1 cut(s) 216
BstDEI CTNAG 1 cut(s) 245
BstEII GGTNACC 1 cut(s) 259
BstF5I GGATG 5 cut(s) 48, 415, 649, 698, 793
BstFNI CGCG 2 cut(s) 272, 274
BstH2I RGCGCY 1 cut(s) 835
BstHHI GCGC 2 cut(s) 274, 834
BstKTI GATC 5 cut(s) 33, 231, 385, 1075, 1090
BstMAI GTCTC 4 cut(s) 261, 372, 841, 884
BstMBI GATC 5 cut(s) 30, 228, 382, 1072, 1087
BstMWI GCNNNNNNNGC 2 cut(s) 185, 216
BstNI CCWGG 1 cut(s) 542
BstPAI GACNNNNGTC 1 cut(s) 170
BstPI GGTNACC 1 cut(s) 259
BstSCI CCNGG 2 cut(s) 540, 973
BstUI CGCG 2 cut(s) 272, 274
BstV1I GCAGC 2 cut(s) 661, 1042
BstV2I GAAGAC 2 cut(s) 144, 957
BstXI CCANNNNNNTGG 1 cut(s) 606
Bsu15I ATCGAT 1 cut(s) 29
BsuI GTATCC 1 cut(s) 759
BsuRI GGCC 2 cut(s) 236, 1106
BsuTUI ATCGAT 1 cut(s) 29
BtsCI GGATG 5 cut(s) 48, 415, 649, 698, 793
BtsI GCAGTG 1 cut(s) 385
BtsIMutI CAGTG 3 cut(s) 385, 564, 1084
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 2 cut(s) 274, 834
Cfr13I GGNCC 4 cut(s) 201, 324, 738, 907
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 2 cut(s) 278, 293
Csp6I GTAC 2 cut(s) 184, 580
CspCI CAANNNNNGTGG 2 cut(s) 290, 325
CviAII CATG 2 cut(s) 170, 656
CviQI GTAC 2 cut(s) 184, 580
DdeI CTNAG 1 cut(s) 245
DpnI GATC 5 cut(s) 32, 230, 384, 1074, 1089
DpnII GATC 5 cut(s) 30, 228, 382, 1072, 1087
DraI TTTAAA 1 cut(s) 1116
DraIII CACNNNGTG 1 cut(s) 294
Eam1104I CTCTTC 1 cut(s) 600
EarI CTCTTC 1 cut(s) 600
Eco47I GGWCC 4 cut(s) 201, 324, 738, 907
Eco47III AGCGCT 1 cut(s) 833
Eco57I CTGAAG 1 cut(s) 298
Eco91I GGTNACC 1 cut(s) 259
EcoO109I RGGNCCY 2 cut(s) 201, 907
EcoO65I GGTNACC 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 540
Esp3I CGTCTC 2 cut(s) 261, 884
FaeI CATG 2 cut(s) 173, 659
FalI AAGNNNNNCTT 2 cut(s) 579, 611
FaqI GGGAC 4 cut(s) 724, 893, 949, 1112
FatI CATG 2 cut(s) 169, 655
FauI CCCGC 1 cut(s) 61
FauNDI CATATG 1 cut(s) 987
FbaI TGATCA 2 cut(s) 382, 1072
Fnu4HI GCNGC 2 cut(s) 675, 1056
FokI GGATG 5 cut(s) 35, 422, 656, 705, 800
Fsp4HI GCNGC 2 cut(s) 675, 1056
GlaI GCGC 2 cut(s) 273, 833
GluI GCNGC 2 cut(s) 675, 1056
GsaI CCCAGC 1 cut(s) 254
HaeII RGCGCY 1 cut(s) 835
HaeIII GGCC 2 cut(s) 236, 1106
HapII CCGG 1 cut(s) 974
HgaI GACGC 2 cut(s) 278, 293
HhaI GCGC 2 cut(s) 274, 834
Hin1I GRCGYC 2 cut(s) 285, 877
Hin1II CATG 2 cut(s) 173, 659
Hin6I GCGC 2 cut(s) 272, 832
HinP1I GCGC 2 cut(s) 272, 832
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 390
HinfI GANTC 7 cut(s) 79, 437, 659, 720, 863, 940, 1030
HpaII CCGG 1 cut(s) 974
HphI GGTGA 3 cut(s) 166, 253, 1061
Hpy166II GTNNAC 2 cut(s) 346, 862
Hpy188I TCNGA 6 cut(s) 78, 90, 246, 328, 565, 621
Hpy188III TCNNGA 4 cut(s) 373, 386, 635, 811
Hpy8I GTNNAC 2 cut(s) 346, 862
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 6 cut(s) 67, 523, 565, 604, 762, 898
HpyCH4III ACNGT 1 cut(s) 1079
HpyCH4IV ACGT 3 cut(s) 94, 115, 877
HpyCH4V TGCA 4 cut(s) 210, 655, 701, 1100
HpyF10VI GCNNNNNNNGC 2 cut(s) 185, 216
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 3 cut(s) 94, 115, 877
Hsp92I GRCGYC 2 cut(s) 285, 877
Hsp92II CATG 2 cut(s) 173, 659
HspAI GCGC 2 cut(s) 272, 832
Ksp22I TGATCA 2 cut(s) 382, 1072
Kzo9I GATC 5 cut(s) 30, 228, 382, 1072, 1087
LmnI GCTCC 2 cut(s) 137, 774
Lsp1109I GCAGC 2 cut(s) 661, 1042
LweI GCATC 3 cut(s) 721, 1042, 1072
MaeII ACGT 3 cut(s) 94, 115, 877
MaeIII GTNAC 3 cut(s) 80, 172, 259
MalI GATC 5 cut(s) 32, 230, 384, 1074, 1089
MboI GATC 5 cut(s) 30, 228, 382, 1072, 1087
MfeI CAATTG 1 cut(s) 15
MluCI AATT 7 cut(s) 15, 72, 400, 413, 669, 870, 912
MlyI GAGTC 4 cut(s) 88, 729, 857, 949
MmeI TCCRAC 2 cut(s) 219, 972
MnlI CCTC 8 cut(s) 214, 226, 240, 424, 427, 778, 1096, 1126
MroXI GAANNNNTTC 2 cut(s) 95, 116
MseI TTAA 4 cut(s) 354, 894, 1092, 1115
MslI CAYNNNNRTG 3 cut(s) 604, 1025, 1080
MspCI CTTAAG 1 cut(s) 1091
MspI CCGG 1 cut(s) 974
MspR9I CCNGG 2 cut(s) 542, 975
MunI CAATTG 1 cut(s) 15
MvaI CCWGG 1 cut(s) 542
MvnI CGCG 2 cut(s) 272, 274
MwoI GCNNNNNNNGC 2 cut(s) 185, 216
NciI CCSGG 1 cut(s) 975
NdeI CATATG 1 cut(s) 987
NdeII GATC 5 cut(s) 30, 228, 382, 1072, 1087
NlaIII CATG 2 cut(s) 173, 659
NlaIV GGNNCC 4 cut(s) 299, 325, 740, 909
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 3 cut(s) 80, 172, 259
PdmI GAANNNNTTC 2 cut(s) 95, 116
PfeI GAWTC 3 cut(s) 437, 659, 1030
PflMI CCANNNNNTGG 1 cut(s) 294
PkrI GCNGC 2 cut(s) 676, 1057
PleI GAGTC 4 cut(s) 87, 728, 857, 948
PpsI GAGTC 4 cut(s) 87, 728, 857, 948
PpuMI RGGWCCY 2 cut(s) 201, 907
PshAI GACNNNNGTC 1 cut(s) 170
Psp1406I AACGTT 2 cut(s) 94, 115
Psp5II RGGWCCY 2 cut(s) 201, 907
Psp6I CCWGG 1 cut(s) 540
PspEI GGTNACC 1 cut(s) 259
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 1 cut(s) 540
PspN4I GGNNCC 4 cut(s) 299, 325, 740, 909
PspPI GGNCC 4 cut(s) 201, 324, 738, 907
PspPPI RGGWCCY 2 cut(s) 201, 907
PstNI CAGNNNCTG 1 cut(s) 563
RsaI GTAC 2 cut(s) 185, 581
RsaNI GTAC 2 cut(s) 184, 580
RseI CAYNNNNRTG 3 cut(s) 604, 1025, 1080
SaqAI TTAA 4 cut(s) 354, 894, 1092, 1115
SatI GCNGC 2 cut(s) 675, 1056
Sau3AI GATC 5 cut(s) 30, 228, 382, 1072, 1087
Sau96I GGNCC 4 cut(s) 201, 324, 738, 907
SchI GAGTC 4 cut(s) 88, 729, 857, 949
ScrFI CCNGG 2 cut(s) 542, 975
SfaNI GCATC 3 cut(s) 721, 1042, 1072
SinI GGWCC 4 cut(s) 201, 324, 738, 907
SmiMI CAYNNNNRTG 3 cut(s) 604, 1025, 1080
SmlI CTYRAG 2 cut(s) 371, 1091
SmoI CTYRAG 2 cut(s) 371, 1091
Sse9I AATT 7 cut(s) 15, 72, 400, 413, 669, 870, 912
SsiI CCGC 2 cut(s) 54, 177
StyD4I CCNGG 2 cut(s) 540, 973
TaaI ACNGT 1 cut(s) 1079
TaiI ACGT 3 cut(s) 97, 118, 880
TaqI TCGA 4 cut(s) 29, 221, 231, 282
TasI AATT 7 cut(s) 15, 72, 400, 413, 669, 870, 912
TatI WGTACW 1 cut(s) 579
TfiI GAWTC 3 cut(s) 437, 659, 1030
Tru1I TTAA 4 cut(s) 354, 894, 1092, 1115
Tru9I TTAA 4 cut(s) 354, 894, 1092, 1115
TscAI CASTG 3 cut(s) 385, 564, 1084
TseFI GTSAC 3 cut(s) 80, 172, 259
TseI GCWGC 2 cut(s) 674, 1055
Tsp45I GTSAC 3 cut(s) 80, 172, 259
TspDTI ATGAA 7 cut(s) 426, 501, 507, 513, 831, 913, 1043
TspGWI ACGGA 1 cut(s) 787
TspRI CASTG 3 cut(s) 385, 564, 1084
Van91I CCANNNNNTGG 1 cut(s) 294
Vha464I CTTAAG 1 cut(s) 1091
VpaK11BI GGWCC 4 cut(s) 201, 324, 738, 907
XapI RAATTY 2 cut(s) 400, 413
XmnI GAANNNNTTC 2 cut(s) 95, 116
ZraI GACGTC 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.