Rorug05G0121400

Polynucleotide kinase 3 phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000926
Physical Location & Seq
Forward (+)
10733172 .. 10736693
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0121400.1

Sequence Viewer

Length: 1140 bp
ATGTCTCACTTGGCTCTCTCTCAGGTTTCACTTACTACTACTGTTCCTGCTGGAAGTGACTTATCTTTGAGAAGATCTGTGTTCAAGGCACAAAGTGTAAATTTCAGTGATAAATCATGGGCACCAGTTTCAACTTTGGACTTGAAAACAAAGAGTGGGTGGCTAAGAAACAAGCGCATAGTATGCATGTCAGTGCAACAAGCAAGTGCACCAAAAGTTGCTGTGTCTCCTTTAGAACTTGACAATGCTACAGAACCCCCATTGAATATATACAAGCCGAAGGAACCTTATACCGCAACCATTTTATCTGTTGAGAGGATTGTCGGCCCAAGGGCTCCTGGAGAAACTTGCCATATAGTGATTGATCATGGTGGTAATGTTCCCTACTGGGAAGGACAAAGTTATGGTGTGATCCCTCCTGGTGAAAACCCAAAGAAACCTGGGGCTCCACACAATGTCCGTCTTTACTCAATTGCATCGACAAGGTATGGAGACTATTTTGATGGCAAGACAACTAGTTTGTGTGTCCGTCGTGCTGTCTATTATGACCCAGAGACAGGGAAGGAGGATCCCTCCAAAAATGGTGTATGCAGCAACTATTTATGTAACTCTAAACCCGGAGATAAAATTAAGATCACAGGTCCTGCTGGCAAGATCATGCTCCTGCCTGAAGACAACCCCAATGCCACCCACATCATGATTGCCACCGGCACTGGTGTTGCTCCATACAGAGGTTACCTTCGTCGTATGTTCATGGAAGCTGTTCCTACATTCAAGTTTGGTGGCCTTGCATGGCTGTTCCTTGGAGTGGCCAATACCGACAGCCTCCTCTATGATGAAGAATTCCGCAAGTATCTCAAGGACTACCCCGATAATTTCCGATACGACATAGCACTTAGTAGAGAACAAAAGAACAAGAGAGGAGGAAAGATGTATGTTCAGGATAAAATTGAAGAATACAGTGATGAGATCTTTAAACTCTTGGATGGTGGGGCCCATATCTACTTCTGTGGGCTCAAGGGTATGATGCCTGGAATTCAAGAAACCTTGAAGAGGGTTGCAGAAGAAAGAGGGGAAAGTTGGGAGGAGAAGCTTTCACAACTGAAGAAGAATAAGCAATGGCATGTTGAAGTTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

379

Amino Acids

42.43

Weight (kDa)

8.86

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 233 - 347 6.5e-25 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 121
AciI CCGC 2 cut(s) 294, 847
AclWI GGATC 3 cut(s) 406, 563, 576
AcoI YGGCCR 1 cut(s) 810
AcsI RAATTY 3 cut(s) 100, 842, 1035
AcuI CTGAAG 2 cut(s) 690, 1124
AdeI CACNNNGTG 1 cut(s) 95
AfiI CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
AgsI TTSAA 9 cut(s) 85, 132, 145, 265, 775, 953, 1040, 1051, 1130
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 4 cut(s) 337, 418, 439, 1030
AluBI AGCT 2 cut(s) 761, 1093
AluI AGCT 2 cut(s) 761, 1093
Alw21I GWGCWC 1 cut(s) 211
Alw26I GTCTC 4 cut(s) 9, 231, 486, 548
Alw44I GTGCAC 1 cut(s) 207
AlwI GGATC 3 cut(s) 406, 563, 576
AlwNI CAGNNNCTG 1 cut(s) 644
AoxI GGCC 4 cut(s) 325, 784, 810, 993
ApaI GGGCCC 1 cut(s) 997
ApaLI GTGCAC 1 cut(s) 207
ApeKI GCWGC 1 cut(s) 591
ApoI RAATTY 3 cut(s) 100, 842, 1035
ArsI GACNNNNNNTTYG 2 cut(s) 502, 534
Asp700I GAANNNNTTC 1 cut(s) 762
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 4 cut(s) 326, 641, 993, 994
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 1 cut(s) 434
AvaII GGWCC 1 cut(s) 641
BaeGI GKGCMC 3 cut(s) 124, 211, 997
BalI TGGCCA 1 cut(s) 812
BamHI GGATCC 1 cut(s) 568
BanI GGYRCC 1 cut(s) 121
BanII GRGCYC 4 cut(s) 337, 448, 997, 1017
BbsI GAAGAC 1 cut(s) 678
Bbv12I GWGCWC 1 cut(s) 211
BbvI GCAGC 1 cut(s) 603
BccI CCATC 2 cut(s) 497, 980
BciT130I CCWGG 4 cut(s) 339, 420, 441, 1032
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 4 cut(s) 9, 231, 486, 548
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 1 cut(s) 249
BglII AGATCT 2 cut(s) 74, 969
BisI GCNGC 1 cut(s) 592
BlsI GCNGC 1 cut(s) 593
Bme1390I CCNGG 5 cut(s) 339, 420, 441, 618, 1032
Bme18I GGWCC 1 cut(s) 641
BmgT120I GGNCC 4 cut(s) 326, 641, 993, 994
BmiI GGNNCC 7 cut(s) 123, 285, 336, 447, 570, 994, 995
BmrFI CCNGG 5 cut(s) 339, 420, 441, 618, 1032
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 2 cut(s) 485, 1017
BmuI ACTGGG 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 678
BplI GAGNNNNNCTC 2 cut(s) 557, 589
BpmI CTGGAG 1 cut(s) 360
BpuEI CTTGAG 2 cut(s) 842, 1001
BpuMI CCSGG 1 cut(s) 618
BsaJI CCNNGG 3 cut(s) 329, 440, 802
Bsc4I CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 3 cut(s) 125, 392, 718
Bse3DI GCAATG 1 cut(s) 1124
BseBI CCWGG 4 cut(s) 339, 420, 441, 1032
BseDI CCNNGG 3 cut(s) 329, 440, 802
BseGI GGATG 1 cut(s) 991
BseLI CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
BseMI GCAATG 1 cut(s) 1124
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 3 cut(s) 125, 392, 718
BseRI GAGGAG 3 cut(s) 818, 936, 1100
BseSI GKGCMC 3 cut(s) 124, 211, 997
BseXI GCAGC 1 cut(s) 603
BshFI GGCC 4 cut(s) 327, 786, 812, 995
BshNI GGYRCC 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 211
BsiSI CCGG 2 cut(s) 618, 708
BslI CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
BsmAI GTCTC 4 cut(s) 9, 231, 486, 548
BsnI GGCC 4 cut(s) 327, 786, 812, 995
Bsp120I GGGCCC 1 cut(s) 993
Bsp1286I GDGCHC 6 cut(s) 124, 211, 337, 448, 997, 1017
Bsp143I GATC 7 cut(s) 74, 364, 411, 568, 633, 654, 969
BspACI CCGC 2 cut(s) 294, 847
BspANI GGCC 4 cut(s) 327, 786, 812, 995
BspCNI CTCAG 1 cut(s) 34
BspHI TCATGA 1 cut(s) 696
BspLI GGNNCC 7 cut(s) 123, 285, 336, 447, 570, 994, 995
BspPI GGATC 3 cut(s) 406, 563, 576
BspT107I GGYRCC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 1124
BsrFI RCCGGY 1 cut(s) 707
BsrI ACTGG 3 cut(s) 125, 392, 718
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 3 cut(s) 329, 440, 802
BssMI GATC 7 cut(s) 74, 364, 411, 568, 633, 654, 969
BssT1I CCWWGG 2 cut(s) 329, 802
Bst2UI CCWGG 4 cut(s) 339, 420, 441, 1032
Bst4CI ACNGT 2 cut(s) 43, 962
Bst6I CTCTTC 1 cut(s) 1046
BstAPI GCANNNNNTGC 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 649
BstDEI CTNAG 3 cut(s) 21, 164, 896
BstEII GGTNACC 1 cut(s) 734
BstENI CCTNNNNNAGG 1 cut(s) 1051
BstF5I GGATG 1 cut(s) 991
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 7 cut(s) 77, 367, 414, 571, 636, 657, 972
BstMAI GTCTC 4 cut(s) 9, 231, 486, 548
BstMBI GATC 7 cut(s) 74, 364, 411, 568, 633, 654, 969
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstNI CCWGG 4 cut(s) 339, 420, 441, 1032
BstNSI RCATGY 2 cut(s) 190, 1127
BstPI GGTNACC 1 cut(s) 734
BstSCI CCNGG 5 cut(s) 337, 418, 439, 616, 1030
BstSFI CTRYAG 1 cut(s) 249
BstSLI GKGCMC 3 cut(s) 124, 211, 997
BstV1I GCAGC 1 cut(s) 603
BstV2I GAAGAC 1 cut(s) 678
BstX2I RGATCY 3 cut(s) 74, 568, 969
BstYI RGATCY 3 cut(s) 74, 568, 969
BsuRI GGCC 4 cut(s) 327, 786, 812, 995
BtsCI GGATG 1 cut(s) 991
BtsIMutI CAGTG 4 cut(s) 112, 198, 711, 967
Cac8I GCNNGC 1 cut(s) 649
CaiI CAGNNNCTG 1 cut(s) 644
CciI TCATGA 1 cut(s) 696
CfoI GCGC 1 cut(s) 177
Cfr10I RCCGGY 1 cut(s) 707
Cfr13I GGNCC 4 cut(s) 326, 641, 993, 994
CspCI CAANNNNNGTGG 2 cut(s) 763, 798
CviAII CATG 8 cut(s) 117, 187, 368, 658, 697, 754, 792, 1124
DdeI CTNAG 3 cut(s) 21, 164, 896
DpnI GATC 7 cut(s) 76, 366, 413, 570, 635, 656, 971
DpnII GATC 7 cut(s) 74, 364, 411, 568, 633, 654, 969
DraI TTTAAA 1 cut(s) 976
DraIII CACNNNGTG 1 cut(s) 95
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 1 cut(s) 1046
EarI CTCTTC 1 cut(s) 1046
Eco130I CCWWGG 2 cut(s) 329, 802
Eco24I GRGCYC 4 cut(s) 337, 448, 997, 1017
Eco47I GGWCC 1 cut(s) 641
Eco57I CTGAAG 2 cut(s) 690, 1124
Eco91I GGTNACC 1 cut(s) 734
EcoNI CCTNNNNNAGG 1 cut(s) 1051
EcoO109I RGGNCCY 2 cut(s) 641, 993
EcoO65I GGTNACC 1 cut(s) 734
EcoRI GAATTC 2 cut(s) 842, 1035
EcoRII CCWGG 4 cut(s) 337, 418, 439, 1030
EcoT14I CCWWGG 2 cut(s) 329, 802
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 4 cut(s) 337, 448, 997, 1017
ErhI CCWWGG 2 cut(s) 329, 802
FaeI CATG 8 cut(s) 120, 190, 371, 661, 700, 757, 795, 1127
FatI CATG 8 cut(s) 116, 186, 367, 657, 696, 753, 791, 1123
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 1 cut(s) 592
FokI GGATG 1 cut(s) 998
FriOI GRGCYC 4 cut(s) 337, 448, 997, 1017
Fsp4HI GCNGC 1 cut(s) 592
FspBI CTAG 1 cut(s) 516
GlaI GCGC 1 cut(s) 176
GluI GCNGC 1 cut(s) 592
GsuI CTGGAG 1 cut(s) 360
HaeIII GGCC 4 cut(s) 327, 786, 812, 995
HapII CCGG 2 cut(s) 618, 708
HhaI GCGC 1 cut(s) 177
Hin1II CATG 8 cut(s) 120, 190, 371, 661, 700, 757, 795, 1127
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 1091
HpaII CCGG 2 cut(s) 618, 708
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 209, 1135
Hpy188I TCNGA 1 cut(s) 881
Hpy188III TCNNGA 3 cut(s) 697, 941, 1040
Hpy8I GTNNAC 2 cut(s) 209, 1135
Hpy99I CGWCG 2 cut(s) 534, 747
HpyAV CCTTC 4 cut(s) 274, 386, 556, 749
HpyCH4III ACNGT 2 cut(s) 43, 962
HpyCH4V TGCA 7 cut(s) 186, 196, 209, 476, 591, 791, 1061
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
HpyF3I CTNAG 3 cut(s) 21, 164, 896
Hsp92II CATG 8 cut(s) 120, 190, 371, 661, 700, 757, 795, 1127
HspAI GCGC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 7 cut(s) 74, 364, 411, 568, 633, 654, 969
LmnI GCTCC 4 cut(s) 340, 451, 666, 727
Lsp1109I GCAGC 1 cut(s) 603
LweI GCATC 2 cut(s) 485, 1017
MaeI CTAG 1 cut(s) 516
MaeIII GTNAC 3 cut(s) 56, 605, 734
MalI GATC 7 cut(s) 76, 366, 413, 570, 635, 656, 971
MboI GATC 7 cut(s) 74, 364, 411, 568, 633, 654, 969
MboII GAAGA 8 cut(s) 84, 683, 851, 965, 1063, 1076, 1117, 1120
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 3 cut(s) 74, 568, 969
MhlI GDGCHC 6 cut(s) 124, 211, 337, 448, 997, 1017
MlsI TGGCCA 1 cut(s) 812
MluCI AATT 7 cut(s) 100, 471, 627, 842, 874, 948, 1035
MluNI TGGCCA 1 cut(s) 812
Mox20I TGGCCA 1 cut(s) 812
Mph1103I ATGCAT 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 762
MscI TGGCCA 1 cut(s) 812
MseI TTAA 2 cut(s) 630, 975
MslI CAYNNNNRTG 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 812
MspI CCGG 2 cut(s) 618, 708
MspR9I CCNGG 5 cut(s) 339, 420, 441, 618, 1032
MunI CAATTG 1 cut(s) 471
MvaI CCWGG 4 cut(s) 339, 420, 441, 1032
MwoI GCNNNNNNNGC 1 cut(s) 183
NciI CCSGG 1 cut(s) 618
NdeII GATC 7 cut(s) 74, 364, 411, 568, 633, 654, 969
NlaIII CATG 8 cut(s) 120, 190, 371, 661, 700, 757, 795, 1127
NlaIV GGNNCC 7 cut(s) 123, 285, 336, 447, 570, 994, 995
NmuCI GTSAC 1 cut(s) 56
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 2 cut(s) 190, 1127
PagI TCATGA 1 cut(s) 696
PdmI GAANNNNTTC 1 cut(s) 762
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 1 cut(s) 593
PpuMI RGGWCCY 1 cut(s) 641
Psp5II RGGWCCY 1 cut(s) 641
Psp6I CCWGG 4 cut(s) 337, 418, 439, 1030
PspEI GGTNACC 1 cut(s) 734
PspGI CCWGG 4 cut(s) 337, 418, 439, 1030
PspN4I GGNNCC 7 cut(s) 123, 285, 336, 447, 570, 994, 995
PspOMI GGGCCC 1 cut(s) 993
PspPI GGNCC 4 cut(s) 326, 641, 993, 994
PspPPI RGGWCCY 1 cut(s) 641
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 3 cut(s) 74, 568, 969
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 2 cut(s) 630, 975
SatI GCNGC 1 cut(s) 592
Sau3AI GATC 7 cut(s) 74, 364, 411, 568, 633, 654, 969
Sau96I GGNCC 4 cut(s) 326, 641, 993, 994
ScrFI CCNGG 5 cut(s) 339, 420, 441, 618, 1032
SduI GDGCHC 6 cut(s) 124, 211, 337, 448, 997, 1017
SfaNI GCATC 2 cut(s) 485, 1017
SfcI CTRYAG 1 cut(s) 249
SinI GGWCC 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 2 cut(s) 857, 1016
SmoI CTYRAG 2 cut(s) 857, 1016
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 7 cut(s) 100, 471, 627, 842, 874, 948, 1035
SsiI CCGC 2 cut(s) 294, 847
SspMI CTAG 1 cut(s) 516
StyD4I CCNGG 5 cut(s) 337, 418, 439, 616, 1030
StyI CCWWGG 2 cut(s) 329, 802
TaaI ACNGT 2 cut(s) 43, 962
TaqI TCGA 1 cut(s) 479
TasI AATT 7 cut(s) 100, 471, 627, 842, 874, 948, 1035
Tru1I TTAA 2 cut(s) 630, 975
Tru9I TTAA 2 cut(s) 630, 975
TscAI CASTG 4 cut(s) 112, 198, 718, 967
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 1 cut(s) 591
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 2 cut(s) 742, 852
TspGWI ACGGA 2 cut(s) 449, 518
TspRI CASTG 4 cut(s) 112, 198, 718, 967
VneI GTGCAC 1 cut(s) 207
VpaK11BI GGWCC 1 cut(s) 641
XagI CCTNNNNNAGG 1 cut(s) 1051
XapI RAATTY 3 cut(s) 100, 842, 1035
XceI RCATGY 2 cut(s) 190, 1127
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 1 cut(s) 516
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.