Rw5G019590

Polynucleotide kinase 3 phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000926
Physical Location & Seq
Reverse (-)
25531554 .. 25534236
2683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G019590.1

Sequence Viewer

Length: 819 bp
ATGTCGTCTTCTTCTTCTTCGACTCAAGTGGCCGCCAATGTCGAAGCCGAGTACGCCAAGTCCGGCAAGTCCTCGTGCAAGAAGTGCTCGAAACCGATCGAGAAGAAAGCTCTGAGGCTAGGCACCAAAGACGCACGTGGTTATACCAAGTGGCACCACTTGTGTTGCTTCAGTTTTGGGTCCGACCCGGTTGCTTCGGTTGACAAGATTAAAGGGTTTCAGTCTCTCAAGAGTAGTGATCAGGAAGCTTTGAAGGATGAATTTGAGAAATCTAAAGAGGAGGATTCAAATGGAAAGAAAAGTTCTAAAAATCGAAAGGTTCGTGAAGTAGATCATGAAGCTGAAGATGAAAATGAAGGGGAAAGGAAGTTGAAGAAATCAAAGGATTCTTCTTCGTCCCATGTCGAAGCCGAGTACGCCAAGTCCAGCAAGTCCTCGTGCAAGAAGTGCTCGAAACCTATCGAGAAGAAAGCTCTGAGGCTGGGCACCAAAGACGCGCGTGGTTATACCAAGTGGCACCACTTGGGTTGCTTCAGTTTTGGGTCCGACCCGGTTGTTTCGGTTGACAAGATCAAAGGATTTCAGTCTCTGGAGAGTAGTGATCAGGAAGCTTTGAAGAATTTGGTGGATGAATTTGAGAAATCTAAAGAGGATTCAAATGGTAAGAGAAGTTCTCAAAAACGAAAGGTTCTTGAATTAGATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGTAGGGGAAAGGGAGTTAAAGAAAGCGAAGGCTATTGGGAGTTCTTTAGGCGTCTTTGTAACTGCCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

272

Amino Acids

30.26

Weight (kDa)

6.17

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-PARP PF00645 17 - 87 3.2e-16 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
zf-PARP PF00645 138 - 208 1.7e-16 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 122, 153, 485, 516
AccII CGCG 2 cut(s) 497, 499
AciI CCGC 1 cut(s) 33
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 3 cut(s) 260, 619, 632
AcuI CTGAAG 3 cut(s) 154, 363, 517
AcvI CACGTG 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 798
AfaI GTAC 2 cut(s) 53, 416
AgsI TTSAA 6 cut(s) 253, 288, 373, 616, 657, 695
AluBI AGCT 5 cut(s) 110, 248, 341, 473, 611
AluI AGCT 5 cut(s) 110, 248, 341, 473, 611
Alw21I GWGCWC 2 cut(s) 89, 452
Alw26I GTCTC 2 cut(s) 228, 591
AlwNI CAGNNNCTG 1 cut(s) 589
AoxI GGCC 1 cut(s) 30
ApoI RAATTY 3 cut(s) 260, 619, 632
AspLEI GCGC 1 cut(s) 499
AspS9I GGNCC 2 cut(s) 180, 543
AsuC2I CCSGG 2 cut(s) 188, 551
AvaII GGWCC 2 cut(s) 180, 543
BaeGI GKGCMC 1 cut(s) 488
BanI GGYRCC 4 cut(s) 122, 153, 485, 516
BauI CACGAG 2 cut(s) 73, 436
BbrPI CACGTG 1 cut(s) 137
Bbv12I GWGCWC 2 cut(s) 89, 452
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BclI TGATCA 2 cut(s) 238, 601
BcnI CCSGG 2 cut(s) 188, 551
BcoDI GTCTC 2 cut(s) 228, 591
BfaI CTAG 1 cut(s) 119
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 2 cut(s) 188, 551
Bme18I GGWCC 2 cut(s) 180, 543
BmgT120I GGNCC 2 cut(s) 180, 543
BmiI GGNNCC 6 cut(s) 124, 155, 181, 487, 518, 544
BmrFI CCNGG 2 cut(s) 188, 551
BplI GAGNNNNNCTC 2 cut(s) 658, 690
BpmI CTGGAG 1 cut(s) 611
BpuEI CTTGAG 2 cut(s) 9, 212
BpuMI CCSGG 2 cut(s) 188, 551
BsaAI YACGTR 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 798
BseGI GGATG 2 cut(s) 262, 634
BseMII CTCAG 2 cut(s) 104, 467
BseRI GAGGAG 1 cut(s) 293
BseSI GKGCMC 1 cut(s) 488
BseYI CCCAGC 1 cut(s) 481
Bsh1236I CGCG 2 cut(s) 497, 499
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 4 cut(s) 122, 153, 485, 516
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 89, 452
BsiSI CCGG 3 cut(s) 63, 188, 551
BslFI GGGAC 1 cut(s) 382
BsmAI GTCTC 2 cut(s) 228, 591
BsmFI GGGAC 1 cut(s) 382
BsnI GGCC 1 cut(s) 32
Bsp1286I GDGCHC 3 cut(s) 89, 452, 488
Bsp143I GATC 5 cut(s) 96, 238, 331, 570, 601
BspACI CCGC 1 cut(s) 33
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 2 cut(s) 105, 468
BspFNI CGCG 2 cut(s) 497, 499
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 6 cut(s) 124, 155, 181, 487, 518, 544
BspT107I GGYRCC 4 cut(s) 122, 153, 485, 516
BssMI GATC 5 cut(s) 96, 238, 331, 570, 601
BssNI GRCGYC 1 cut(s) 798
BssSI CACGAG 2 cut(s) 73, 436
Bst2BI CACGAG 2 cut(s) 73, 436
BstACI GRCGYC 1 cut(s) 798
BstAPI GCANNNNNTGC 2 cut(s) 84, 447
BstBAI YACGTR 1 cut(s) 137
BstDEI CTNAG 2 cut(s) 113, 476
BstF5I GGATG 2 cut(s) 262, 634
BstFNI CGCG 2 cut(s) 497, 499
BstHHI GCGC 1 cut(s) 499
BstKTI GATC 5 cut(s) 99, 241, 334, 573, 604
BstMAI GTCTC 2 cut(s) 228, 591
BstMBI GATC 5 cut(s) 96, 238, 331, 570, 601
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 4 cut(s) 53, 84, 416, 447
BstSCI CCNGG 2 cut(s) 186, 549
BstSLI GKGCMC 1 cut(s) 488
BstUI CGCG 2 cut(s) 497, 499
BsuRI GGCC 1 cut(s) 32
BtsCI GGATG 2 cut(s) 262, 634
CaiI CAGNNNCTG 1 cut(s) 589
CciI TCATGA 1 cut(s) 334
CfoI GCGC 1 cut(s) 499
Cfr13I GGNCC 2 cut(s) 180, 543
CseI GACGC 3 cut(s) 140, 503, 787
Csp6I GTAC 2 cut(s) 52, 415
CspCI CAANNNNNGTGG 4 cut(s) 146, 181, 509, 544
CviAII CATG 2 cut(s) 335, 401
CviQI GTAC 2 cut(s) 52, 415
DdeI CTNAG 2 cut(s) 113, 476
DpnI GATC 5 cut(s) 98, 240, 333, 572, 603
DpnII GATC 5 cut(s) 96, 238, 331, 570, 601
EaeI YGGCCR 1 cut(s) 30
Eco47I GGWCC 2 cut(s) 180, 543
Eco57I CTGAAG 3 cut(s) 154, 363, 517
Eco72I CACGTG 1 cut(s) 137
FaeI CATG 2 cut(s) 338, 404
FaiI YATR 4 cut(s) 144, 336, 402, 507
FaqI GGGAC 1 cut(s) 382
FatI CATG 2 cut(s) 334, 400
FbaI TGATCA 2 cut(s) 238, 601
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 2 cut(s) 269, 641
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 1 cut(s) 119
GlaI GCGC 1 cut(s) 498
GluI GCNGC 1 cut(s) 33
GsaI CCCAGC 1 cut(s) 485
GsuI CTGGAG 1 cut(s) 611
HaeIII GGCC 1 cut(s) 32
HapII CCGG 3 cut(s) 63, 188, 551
HgaI GACGC 3 cut(s) 140, 503, 787
HhaI GCGC 1 cut(s) 499
Hin1I GRCGYC 1 cut(s) 798
Hin1II CATG 2 cut(s) 338, 404
Hin6I GCGC 1 cut(s) 497
HinP1I GCGC 1 cut(s) 497
HincII GTYRAC 2 cut(s) 202, 565
HindII GTYRAC 2 cut(s) 202, 565
HindIII AAGCTT 2 cut(s) 246, 609
HinfI GANTC 4 cut(s) 22, 284, 386, 653
HpaII CCGG 3 cut(s) 63, 188, 551
Hpy166II GTNNAC 2 cut(s) 202, 565
Hpy188I TCNGA 4 cut(s) 114, 184, 477, 547
Hpy188III TCNNGA 9 cut(s) 100, 229, 242, 323, 335, 463, 590, 605, 692
Hpy8I GTNNAC 2 cut(s) 202, 565
HpyAV CCTTC 3 cut(s) 247, 350, 769
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 2 cut(s) 78, 441
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 84, 416, 447
HpyF3I CTNAG 2 cut(s) 113, 476
HpySE526I ACGT 1 cut(s) 136
Hsp92I GRCGYC 1 cut(s) 798
Hsp92II CATG 2 cut(s) 338, 404
HspAI GCGC 1 cut(s) 497
Ksp22I TGATCA 2 cut(s) 238, 601
Kzo9I GATC 5 cut(s) 96, 238, 331, 570, 601
LpnPI CCDG 8 cut(s) 76, 201, 227, 439, 467, 564, 575, 590
MaeI CTAG 1 cut(s) 119
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 805
MalI GATC 5 cut(s) 98, 240, 333, 572, 603
MboI GATC 5 cut(s) 96, 238, 331, 570, 601
MhlI GDGCHC 3 cut(s) 89, 452, 488
MluCI AATT 4 cut(s) 260, 619, 632, 695
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 2 cut(s) 207, 570
MnlI CCTC 7 cut(s) 82, 108, 271, 274, 445, 471, 643
MseI TTAA 3 cut(s) 210, 764, 817
MspI CCGG 3 cut(s) 63, 188, 551
MspR9I CCNGG 2 cut(s) 188, 551
MvnI CGCG 2 cut(s) 497, 499
MwoI GCNNNNNNNGC 4 cut(s) 53, 84, 416, 447
NciI CCSGG 2 cut(s) 188, 551
NdeII GATC 5 cut(s) 96, 238, 331, 570, 601
NlaIII CATG 2 cut(s) 338, 404
NlaIV GGNNCC 6 cut(s) 124, 155, 181, 487, 518, 544
NmeAIII GCCGAG 2 cut(s) 73, 436
PagI TCATGA 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 319
PfeI GAWTC 3 cut(s) 284, 386, 653
PkrI GCNGC 1 cut(s) 34
Ple19I CGATCG 1 cut(s) 99
PleI GAGTC 1 cut(s) 16
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
PpsI GAGTC 1 cut(s) 16
Ppu21I YACGTR 1 cut(s) 137
PspCI CACGTG 1 cut(s) 137
PspFI CCCAGC 1 cut(s) 481
PspN4I GGNNCC 6 cut(s) 124, 155, 181, 487, 518, 544
PspPI GGNCC 2 cut(s) 180, 543
PsrI GAACNNNNNNTAC 2 cut(s) 655, 687
PstNI CAGNNNCTG 1 cut(s) 589
PvuI CGATCG 1 cut(s) 99
RsaI GTAC 2 cut(s) 53, 416
RsaNI GTAC 2 cut(s) 52, 415
SaqAI TTAA 3 cut(s) 210, 764, 817
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 5 cut(s) 96, 238, 331, 570, 601
Sau96I GGNCC 2 cut(s) 180, 543
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 188, 551
SduI GDGCHC 3 cut(s) 89, 452, 488
SetI ASST 9 cut(s) 112, 139, 250, 321, 343, 460, 475, 613, 690
SinI GGWCC 2 cut(s) 180, 543
SmlI CTYRAG 2 cut(s) 24, 227
SmoI CTYRAG 2 cut(s) 24, 227
Sse9I AATT 4 cut(s) 260, 619, 632, 695
SsiI CCGC 1 cut(s) 33
SspMI CTAG 1 cut(s) 119
StyD4I CCNGG 2 cut(s) 186, 549
TaiI ACGT 1 cut(s) 139
TaqI TCGA 8 cut(s) 20, 42, 89, 99, 313, 405, 452, 462
TasI AATT 4 cut(s) 260, 619, 632, 695
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 3 cut(s) 284, 386, 653
Tru1I TTAA 3 cut(s) 210, 764, 817
Tru9I TTAA 3 cut(s) 210, 764, 817
VpaK11BI GGWCC 2 cut(s) 180, 543
XapI RAATTY 3 cut(s) 260, 619, 632
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.