Rroxscaffold_1G00049380

Polynucleotide kinase 3 phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000926
Physical Location & Seq
Forward (+)
69561754 .. 69570626
8873 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00049380.1

Sequence Viewer

Length: 2100 bp
ATGTCTTCTTCTTCTTCTTCGACTCAAGTGGCCGCCAATGTCGAAGCCGAGTACGCCAAGTCCGGCAAGTCCTTGTGCAAGAAGTGCTCGGAACCAATCGAGAAGAAAGCTCTGAGGCTGGGCACCAAAGACGCGCGTGGTTATACCAAGTGGCACCACTTGTGTTGCTTCAGTTTTGGGTCCGAGCCGGTTGCTTCGGTTGACAAGATTAAAGGGTTTCAGTCTCTCAAGAGTAGTGATCAGGAAGCTTTGAAGGATGAATTTGAGAAATCTAAAGAGGAGGATTCAAATGGAAAGAAAAGTTCTCAAAATCGAAAGGTTCGTGAAGTAGATCATGAAGCTGAAGATGAAAATGTAGGGGAAAGGAAGTTGAAGAAATCAAAGGATTCTTCTTCGACCCATGTCGAAGCCGAGTATGCCAAGTCCAGCAAGTCCTCCTGCAAGAAGTGCTCGAAACCGATCGAGAAGAAAGCTCTGAGGCTGGGCACCAAAGACGCGCGTGGTTATACCAAGTGGCACCACTTGGGTTGCTTCTCTTTTGGGTCTGAGCCGGTTGCTTCGGTTGACAAGATTATAGGATTTCAGTCTCTGGAGAGTAGTGATCAGGAAGCTTTGAAGAATTTGGTGGATGAATTTGAGAAATCTAAAGAGAATTCAAATGGTAAGAGAAGTTCTCAAAAACGAAAGGTTCTTGAATTAGATGAAGATGAAGATGAAGATGAAGAGGAAGATGAAGATGAAGATGAAGAGGAAGATGAAAATGAAGTAGGGGAAAGGGAGTTGAAGAAAGTGAAGAATAGGTTCTTGATGCATGTGATGATTGAGGTGATTTCTGGAGGTTTGAAGGCTGGTTTTATCGAATTTGGCATAGGTCTTGCTCGATCCATTGCAATGGAAGGCTATTACCGCTTTCACGATGGAATGGAGGCTCTGTCTTTCACAACGGAGAGTGGGTTGAGGAGTTATTACGAGGGGAATTGGGATGGTGGTGAGTTCCATTGCCTGGTGTGTGGTGGGTTGGGGAAGACGAAGAAGATCAAGGGTTGTGTAGGGCTTGTTCACCACGCGGTTTCGATAAGCAAGACGAAGAAGAAGAGAGCTCACAGGGCTTTTGCTCAGGGTGAGAGTGAGGTAAATGCTGGTGCTAGTGAGGTTGTTTCGGGTGCTGTTAAAGACAATGTGGGAGATGAAAGGGATGGTAATGCCGAGTCAGGTGAAAAGGAAGCATCTAATGGAGACCTAATGATTTGTCAGAAATCTTTGAAAGATGATGATGCAAATCAATACAAAGAAACCCTTAATAAGGGGATATCAGAAATCAAAGAAGCGAAAGAAGAGGCAATGGATACGCGGAGTGAGCCCGAGTTAAATATTGACCAGTTGGATAGTGCACTGAAAGACAATGTGGCAGCTGAAACTGATGCTCCTGCTGAATCTAGTGAAAAGGAGGCATTTGATGATCATCAGTGCAATGGTAAAGAACCAATCATCTGTCAGAGCTCTTTGAAAGATGATGGTACAGATAAAAGCAGAGAAAGCCTTGATGAGGGAAAGACAGTATTAATAAAGGAAGCAATTGGAGAAACAGCAAAGGATGCATTGGGTGAGCCTGAGGTACGTGCTGGTTCTTGTGAGGGTGTTTTTTGTGTTGTTAAAGACAATGTGGTAGCCGAAAATGATGCCAATGCTGAATTGGGTGTAAAAGAGGTATTCGATGATCATCAGATTAACAGTGAAAATAAAATGATTTGTCAGGTTGTATCGTTTCAGAACTTGTTGAAAGATGATGGTACAACAGAAAACACAGAAGCCCTCAACAAGGGAATATCAGAAGTCGAAGCAAACAAAGAAGATGGAGTGGATACAGGGGTTGAAGTTCCGGAAAATGCTGATGATTACAAGCATGATTTAGTTGTTTCCAAAGAAACATTGGATACTATCAGTGGATATACTGGTGAAATAGGCTCAAACGTGGAACCTGAGGTAGCCTTAGGTGATGGAACACCAATGGCATGTGAGTTGCAGGCTGAACCTGAGAAAAATGTGGATGGTCCCAATGATGATTTAAATGTCTTTTTGGAGAATTTAGAACCCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

699

Amino Acids

76.6

Weight (kDa)

4.73

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-PARP PF00645 17 - 87 3.8e-15 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
zf-PARP PF00645 138 - 208 1.9e-15 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 122, 153, 485, 516
AccB7I CCANNNNNTGG 1 cut(s) 1003
AccII CGCG 6 cut(s) 134, 136, 497, 499, 1067, 1351
AccIII TCCGGA 1 cut(s) 1879
AciI CCGC 4 cut(s) 33, 907, 1067, 1351
AclWI GGATC 1 cut(s) 876
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 6 cut(s) 260, 619, 632, 652, 860, 2083
AcuI CTGAAG 2 cut(s) 154, 363
AfaI GTAC 4 cut(s) 53, 1519, 1617, 1792
AfiI CCNNNNNNNGG 4 cut(s) 1003, 1303, 1546, 1819
AjnI CCWGG 1 cut(s) 1002
AluBI AGCT 8 cut(s) 110, 248, 341, 473, 611, 1100, 1412, 1500
AluI AGCT 8 cut(s) 110, 248, 341, 473, 611, 1100, 1412, 1500
Alw21I GWGCWC 5 cut(s) 89, 452, 1102, 1393, 1502
Alw26I GTCTC 3 cut(s) 228, 591, 1230
Alw44I GTGCAC 1 cut(s) 1389
AlwI GGATC 1 cut(s) 876
AlwNI CAGNNNCTG 1 cut(s) 589
Ama87I CYCGRG 1 cut(s) 1361
Aor13HI TCCGGA 1 cut(s) 1879
AoxI GGCC 1 cut(s) 30
ApaLI GTGCAC 1 cut(s) 1389
ApeKI GCWGC 1 cut(s) 1409
ApoI RAATTY 6 cut(s) 260, 619, 632, 652, 860, 2083
AseI ATTAAT 1 cut(s) 1562
Asp700I GAANNNNTTC 1 cut(s) 800
AspLEI GCGC 2 cut(s) 136, 499
AspS9I GGNCC 2 cut(s) 180, 2051
AsuHPI GGTGA 8 cut(s) 838, 1001, 1052, 1133, 1226, 1616, 1967, 2006
AvaI CYCGRG 1 cut(s) 1361
AvaII GGWCC 2 cut(s) 180, 2051
AxyI CCTNAGG 3 cut(s) 1611, 1980, 1990
BaeGI GKGCMC 3 cut(s) 125, 488, 1393
BaeI ACNNNNGTAYC 2 cut(s) 1854, 1887
BanI GGYRCC 4 cut(s) 122, 153, 485, 516
BanII GRGCYC 3 cut(s) 1102, 1362, 1502
BbsI GAAGAC 1 cut(s) 1031
Bbv12I GWGCWC 5 cut(s) 89, 452, 1102, 1393, 1502
BbvI GCAGC 1 cut(s) 1421
BccI CCATC 8 cut(s) 911, 977, 1190, 1508, 1781, 1847, 1991, 2042
BcgI CGANNNNNNTGC 4 cut(s) 173, 207, 1661, 1695
BciT130I CCWGG 1 cut(s) 1004
BciVI GTATCC 3 cut(s) 1339, 1855, 1927
BclI TGATCA 4 cut(s) 238, 601, 1459, 1717
BcoDI GTCTC 3 cut(s) 228, 591, 1230
BfaI CTAG 2 cut(s) 1146, 1437
BfuI GTATCC 3 cut(s) 1339, 1855, 1927
BisI GCNGC 2 cut(s) 33, 1410
BlsI GCNGC 2 cut(s) 34, 1411
Bme1390I CCNGG 1 cut(s) 1004
Bme18I GGWCC 2 cut(s) 180, 2051
BmeT110I CYCGRG 1 cut(s) 1361
BmgT120I GGNCC 2 cut(s) 180, 2051
BmiI GGNNCC 8 cut(s) 93, 124, 155, 181, 487, 518, 1977, 2053
BmrFI CCNGG 1 cut(s) 1004
BmsI GCATC 6 cut(s) 798, 1235, 1264, 1411, 1585, 1669
BoxI GACNNNNGTC 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 1031
BplI GAGNNNNNCTC 2 cut(s) 658, 690
BpmI CTGGAG 2 cut(s) 611, 855
Bpu10I CCTNAGC 1 cut(s) 1116
BpuEI CTTGAG 2 cut(s) 9, 212
BsaAI YACGTR 1 cut(s) 1619
BsaBI GATNNNNATC 3 cut(s) 1278, 1461, 1719
BsaI GGTCTC 1 cut(s) 1230
BsaWI WCCGGW 1 cut(s) 1879
BsaXI ACNNNNNCTCC 6 cut(s) 917, 938, 947, 968, 1408, 1438
Bsc4I CCNNNNNNNGG 4 cut(s) 1003, 1303, 1546, 1819
Bse118I RCCGGY 2 cut(s) 187, 550
Bse1I ACTGG 2 cut(s) 1378, 1957
Bse21I CCTNAGG 3 cut(s) 1611, 1980, 1990
Bse3DI GCAATG 5 cut(s) 885, 897, 997, 1347, 1477
Bse8I GATNNNNATC 3 cut(s) 1278, 1461, 1719
BseAI TCCGGA 1 cut(s) 1879
BseBI CCWGG 1 cut(s) 1004
BseGI GGATG 6 cut(s) 262, 634, 988, 1201, 1600, 2053
BseJI GATNNNNATC 3 cut(s) 1278, 1461, 1719
BseLI CCNNNNNNNGG 4 cut(s) 1003, 1303, 1546, 1819
BseMI GCAATG 5 cut(s) 885, 897, 997, 1347, 1477
BseMII CTCAG 7 cut(s) 104, 467, 537, 1130, 1602, 1971, 2025
BseNI ACTGG 2 cut(s) 1378, 1957
BseRI GAGGAG 2 cut(s) 293, 973
BseSI GKGCMC 3 cut(s) 125, 488, 1393
BseXI GCAGC 1 cut(s) 1421
BseYI CCCAGC 2 cut(s) 118, 481
Bsh1236I CGCG 6 cut(s) 134, 136, 497, 499, 1067, 1351
Bsh1285I CGRYCG 1 cut(s) 462
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 4 cut(s) 122, 153, 485, 516
BsiEI CGRYCG 1 cut(s) 462
BsiHKAI GWGCWC 5 cut(s) 89, 452, 1102, 1393, 1502
BsiHKCI CYCGRG 1 cut(s) 1361
BsiSI CCGG 4 cut(s) 63, 188, 551, 1880
BslFI GGGAC 1 cut(s) 2037
BslI CCNNNNNNNGG 4 cut(s) 1003, 1303, 1546, 1819
BsmAI GTCTC 3 cut(s) 228, 591, 1230
BsmFI GGGAC 1 cut(s) 2037
BsnI GGCC 1 cut(s) 32
Bso31I GGTCTC 1 cut(s) 1230
BsoBI CYCGRG 1 cut(s) 1361
Bsp1286I GDGCHC 8 cut(s) 89, 125, 452, 488, 1102, 1362, 1393, 1502
Bsp13I TCCGGA 1 cut(s) 1879
Bsp143I GATC 8 cut(s) 238, 331, 459, 601, 881, 1035, 1459, 1717
BspACI CCGC 4 cut(s) 33, 907, 1067, 1351
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 7 cut(s) 105, 468, 538, 1129, 1603, 1972, 2026
BspEI TCCGGA 1 cut(s) 1879
BspFNI CGCG 6 cut(s) 134, 136, 497, 499, 1067, 1351
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 8 cut(s) 93, 124, 155, 181, 487, 518, 1977, 2053
BspPI GGATC 1 cut(s) 876
BspT107I GGYRCC 4 cut(s) 122, 153, 485, 516
BspTNI GGTCTC 1 cut(s) 1230
BsrDI GCAATG 5 cut(s) 885, 897, 997, 1347, 1477
BsrFI RCCGGY 2 cut(s) 187, 550
BsrI ACTGG 2 cut(s) 1378, 1957
BssAI RCCGGY 2 cut(s) 187, 550
BssMI GATC 8 cut(s) 238, 331, 459, 601, 881, 1035, 1459, 1717
Bst2UI CCWGG 1 cut(s) 1004
Bst4CI ACNGT 2 cut(s) 1558, 1733
Bst6I CTCTTC 4 cut(s) 717, 741, 1088, 1329
BstAPI GCANNNNNTGC 3 cut(s) 84, 447, 1595
BstBAI YACGTR 1 cut(s) 1619
BstC8I GCNNGC 1 cut(s) 2025
BstDEI CTNAG 8 cut(s) 113, 476, 546, 1116, 1611, 1980, 1990, 2034
BstENI CCTNNNNNAGG 3 cut(s) 1301, 1544, 1817
BstF5I GGATG 6 cut(s) 262, 634, 988, 1201, 1600, 2053
BstFNI CGCG 6 cut(s) 134, 136, 497, 499, 1067, 1351
BstHHI GCGC 2 cut(s) 136, 499
BstKTI GATC 8 cut(s) 241, 334, 462, 604, 884, 1038, 1462, 1720
BstMAI GTCTC 3 cut(s) 228, 591, 1230
BstMBI GATC 8 cut(s) 238, 331, 459, 601, 881, 1035, 1459, 1717
BstMCI CGRYCG 1 cut(s) 462
BstMWI GCNNNNNNNGC 9 cut(s) 53, 84, 416, 447, 906, 1106, 1357, 1536, 1595
BstNI CCWGG 1 cut(s) 1004
BstNSI RCATGY 2 cut(s) 815, 2016
BstPAI GACNNNNGTC 1 cut(s) 401
BstSCI CCNGG 1 cut(s) 1002
BstSLI GKGCMC 3 cut(s) 125, 488, 1393
BstUI CGCG 6 cut(s) 134, 136, 497, 499, 1067, 1351
BstV1I GCAGC 1 cut(s) 1421
BstV2I GAAGAC 1 cut(s) 1031
BstXI CCANNNNNNTGG 1 cut(s) 892
Bsu36I CCTNAGG 3 cut(s) 1611, 1980, 1990
BsuI GTATCC 3 cut(s) 1339, 1855, 1927
BsuRI GGCC 1 cut(s) 32
BtsCI GGATG 6 cut(s) 262, 634, 988, 1201, 1600, 2053
BtsIMutI CAGTG 4 cut(s) 1391, 1472, 1738, 1948
Cac8I GCNNGC 1 cut(s) 2025
CaiI CAGNNNCTG 1 cut(s) 589
CciI TCATGA 1 cut(s) 334
CfoI GCGC 2 cut(s) 136, 499
Cfr10I RCCGGY 2 cut(s) 187, 550
Cfr13I GGNCC 2 cut(s) 180, 2051
CseI GACGC 2 cut(s) 140, 503
Csp6I GTAC 4 cut(s) 52, 1518, 1616, 1791
CspCI CAANNNNNGTGG 4 cut(s) 146, 181, 509, 544
CviAII CATG 5 cut(s) 335, 401, 812, 1904, 2013
CviQI GTAC 4 cut(s) 52, 1518, 1616, 1791
DdeI CTNAG 8 cut(s) 113, 476, 546, 1116, 1611, 1980, 1990, 2034
DpnI GATC 8 cut(s) 240, 333, 461, 603, 883, 1037, 1461, 1719
DpnII GATC 8 cut(s) 238, 331, 459, 601, 881, 1035, 1459, 1717
DraI TTTAAA 1 cut(s) 2067
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 4 cut(s) 717, 741, 1088, 1329
EarI CTCTTC 4 cut(s) 717, 741, 1088, 1329
Ecl136II GAGCTC 2 cut(s) 1100, 1500
Eco24I GRGCYC 3 cut(s) 1102, 1362, 1502
Eco31I GGTCTC 1 cut(s) 1230
Eco32I GATATC 1 cut(s) 1311
Eco47I GGWCC 2 cut(s) 180, 2051
Eco53kI GAGCTC 2 cut(s) 1100, 1500
Eco57I CTGAAG 2 cut(s) 154, 363
Eco81I CCTNAGG 3 cut(s) 1611, 1980, 1990
Eco88I CYCGRG 1 cut(s) 1361
EcoICRI GAGCTC 2 cut(s) 1100, 1500
EcoNI CCTNNNNNAGG 3 cut(s) 1301, 1544, 1817
EcoRI GAATTC 1 cut(s) 652
EcoRII CCWGG 1 cut(s) 1002
EcoRV GATATC 1 cut(s) 1311
EcoT22I ATGCAT 2 cut(s) 813, 1600
EcoT38I GRGCYC 3 cut(s) 1102, 1362, 1502
FaeI CATG 5 cut(s) 338, 404, 815, 1907, 2016
FalI AAGNNNNNCTT 2 cut(s) 1281, 1313
FaqI GGGAC 1 cut(s) 2037
FatI CATG 5 cut(s) 334, 400, 811, 1903, 2012
FbaI TGATCA 4 cut(s) 238, 601, 1459, 1717
Fnu4HI GCNGC 2 cut(s) 33, 1410
FokI GGATG 6 cut(s) 269, 641, 995, 1208, 1607, 2060
FriOI GRGCYC 3 cut(s) 1102, 1362, 1502
Fsp4HI GCNGC 2 cut(s) 33, 1410
FspBI CTAG 2 cut(s) 1146, 1437
GlaI GCGC 2 cut(s) 135, 498
GluI GCNGC 2 cut(s) 33, 1410
GsaI CCCAGC 2 cut(s) 122, 485
GsuI CTGGAG 2 cut(s) 611, 855
HaeIII GGCC 1 cut(s) 32
HapII CCGG 4 cut(s) 63, 188, 551, 1880
HgaI GACGC 2 cut(s) 140, 503
HhaI GCGC 2 cut(s) 136, 499
Hin1II CATG 5 cut(s) 338, 404, 815, 1907, 2016
Hin6I GCGC 2 cut(s) 134, 497
HinP1I GCGC 2 cut(s) 134, 497
HincII GTYRAC 2 cut(s) 202, 565
HindII GTYRAC 2 cut(s) 202, 565
HindIII AAGCTT 2 cut(s) 246, 609
HinfI GANTC 5 cut(s) 22, 284, 386, 1208, 1433
HpaII CCGG 4 cut(s) 63, 188, 551, 1880
HphI GGTGA 8 cut(s) 838, 1001, 1052, 1133, 1226, 1616, 1967, 2006
Hpy166II GTNNAC 4 cut(s) 202, 565, 1060, 1391
Hpy8I GTNNAC 4 cut(s) 202, 565, 1060, 1391
HpyAV CCTTC 3 cut(s) 247, 838, 890
HpyCH4III ACNGT 2 cut(s) 1558, 1733
HpyCH4IV ACGT 2 cut(s) 1618, 1971
HpyCH4V TGCA 9 cut(s) 78, 441, 811, 890, 1277, 1391, 1470, 1598, 2023
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 84, 416, 447, 906, 1106, 1357, 1536, 1595
HpyF3I CTNAG 8 cut(s) 113, 476, 546, 1116, 1611, 1980, 1990, 2034
HpySE526I ACGT 2 cut(s) 1618, 1971
Hsp92II CATG 5 cut(s) 338, 404, 815, 1907, 2016
HspAI GCGC 2 cut(s) 134, 497
Kpn2I TCCGGA 1 cut(s) 1879
Ksp22I TGATCA 4 cut(s) 238, 601, 1459, 1717
Kzo9I GATC 8 cut(s) 238, 331, 459, 601, 881, 1035, 1459, 1717
LmnI GCTCC 1 cut(s) 1429
Lsp1109I GCAGC 1 cut(s) 1421
LweI GCATC 6 cut(s) 798, 1235, 1264, 1411, 1585, 1669
MaeI CTAG 2 cut(s) 1146, 1437
MaeII ACGT 2 cut(s) 1618, 1971
MalI GATC 8 cut(s) 240, 333, 461, 603, 883, 1037, 1461, 1719
MboI GATC 8 cut(s) 238, 331, 459, 601, 881, 1035, 1459, 1717
MfeI CAATTG 1 cut(s) 1575
MhlI GDGCHC 8 cut(s) 89, 125, 452, 488, 1102, 1362, 1393, 1502
MlyI GAGTC 2 cut(s) 16, 1217
MmeI TCCRAC 1 cut(s) 1362
Mph1103I ATGCAT 2 cut(s) 813, 1600
MroI TCCGGA 1 cut(s) 1879
MroXI GAANNNNTTC 1 cut(s) 800
MseI TTAA 8 cut(s) 210, 1170, 1299, 1367, 1562, 1653, 1728, 2066
MslI CAYNNNNRTG 1 cut(s) 890
MspA1I CMGCKG 1 cut(s) 1412
MspI CCGG 4 cut(s) 63, 188, 551, 1880
MspR9I CCNGG 1 cut(s) 1004
MunI CAATTG 1 cut(s) 1575
MvaI CCWGG 1 cut(s) 1004
MvnI CGCG 6 cut(s) 134, 136, 497, 499, 1067, 1351
MwoI GCNNNNNNNGC 9 cut(s) 53, 84, 416, 447, 906, 1106, 1357, 1536, 1595
NdeII GATC 8 cut(s) 238, 331, 459, 601, 881, 1035, 1459, 1717
NlaIII CATG 5 cut(s) 338, 404, 815, 1907, 2016
NlaIV GGNNCC 8 cut(s) 93, 124, 155, 181, 487, 518, 1977, 2053
NmeAIII GCCGAG 3 cut(s) 73, 436, 1231
NsiI ATGCAT 2 cut(s) 813, 1600
NspI RCATGY 2 cut(s) 815, 2016
PagI TCATGA 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 319
PdmI GAANNNNTTC 1 cut(s) 800
PfeI GAWTC 3 cut(s) 284, 386, 1433
PflMI CCANNNNNTGG 1 cut(s) 1003
PkrI GCNGC 2 cut(s) 34, 1411
Ple19I CGATCG 1 cut(s) 462
PleI GAGTC 2 cut(s) 16, 1216
PpsI GAGTC 2 cut(s) 16, 1216
Ppu21I YACGTR 1 cut(s) 1619
PshAI GACNNNNGTC 1 cut(s) 401
PshBI ATTAAT 1 cut(s) 1562
Psp124BI GAGCTC 2 cut(s) 1102, 1502
Psp6I CCWGG 1 cut(s) 1002
PspFI CCCAGC 2 cut(s) 118, 481
PspGI CCWGG 1 cut(s) 1002
PspN4I GGNNCC 8 cut(s) 93, 124, 155, 181, 487, 518, 1977, 2053
PspPI GGNCC 2 cut(s) 180, 2051
PsrI GAACNNNNNNTAC 4 cut(s) 655, 687, 1041, 1073
PstNI CAGNNNCTG 1 cut(s) 589
PvuI CGATCG 1 cut(s) 462
PvuII CAGCTG 1 cut(s) 1412
RsaI GTAC 4 cut(s) 53, 1519, 1617, 1792
RsaNI GTAC 4 cut(s) 52, 1518, 1616, 1791
RseI CAYNNNNRTG 1 cut(s) 890
SacI GAGCTC 2 cut(s) 1102, 1502
SaqAI TTAA 8 cut(s) 210, 1170, 1299, 1367, 1562, 1653, 1728, 2066
SatI GCNGC 2 cut(s) 33, 1410
Sau3AI GATC 8 cut(s) 238, 331, 459, 601, 881, 1035, 1459, 1717
Sau96I GGNCC 2 cut(s) 180, 2051
SchI GAGTC 2 cut(s) 16, 1217
ScrFI CCNGG 1 cut(s) 1004
SduI GDGCHC 8 cut(s) 89, 125, 452, 488, 1102, 1362, 1393, 1502
SfaNI GCATC 6 cut(s) 798, 1235, 1264, 1411, 1585, 1669
SinI GGWCC 2 cut(s) 180, 2051
SmiI ATTTAAAT 1 cut(s) 2067
SmiMI CAYNNNNRTG 1 cut(s) 890
SmlI CTYRAG 2 cut(s) 24, 227
SmoI CTYRAG 2 cut(s) 24, 227
SsiI CCGC 4 cut(s) 33, 907, 1067, 1351
SspI AATATT 1 cut(s) 1372
SspMI CTAG 2 cut(s) 1146, 1437
SstI GAGCTC 2 cut(s) 1102, 1502
StyD4I CCNGG 1 cut(s) 1002
SwaI ATTTAAAT 1 cut(s) 2067
TaaI ACNGT 2 cut(s) 1558, 1733
TaiI ACGT 2 cut(s) 1621, 1974
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 3 cut(s) 284, 386, 1433
Tru1I TTAA 8 cut(s) 210, 1170, 1299, 1367, 1562, 1653, 1728, 2066
Tru9I TTAA 8 cut(s) 210, 1170, 1299, 1367, 1562, 1653, 1728, 2066
TscAI CASTG 4 cut(s) 1398, 1472, 1738, 1948
TseI GCWGC 1 cut(s) 1409
TspGWI ACGGA 1 cut(s) 959
TspRI CASTG 4 cut(s) 1398, 1472, 1738, 1948
Van91I CCANNNNNTGG 1 cut(s) 1003
VneI GTGCAC 1 cut(s) 1389
VpaK11BI GGWCC 2 cut(s) 180, 2051
VspI ATTAAT 1 cut(s) 1562
XagI CCTNNNNNAGG 3 cut(s) 1301, 1544, 1817
XapI RAATTY 6 cut(s) 260, 619, 632, 652, 860, 2083
XceI RCATGY 2 cut(s) 815, 2016
XcmI CCANNNNNNNNNTGG 2 cut(s) 1690, 1927
XmnI GAANNNNTTC 1 cut(s) 800
XspI CTAG 2 cut(s) 1146, 1437
Zsp2I ATGCAT 2 cut(s) 813, 1600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.