Rh5AG214400

Polynucleotide kinase 3 phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000926
Physical Location & Seq
Reverse (-)
25236187 .. 25238650
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG214400.1

Sequence Viewer

Length: 1008 bp
ATGTCGTCTTCTTCTTCTTCGACTCAAGTGGCCGCCAATGTCGAAGCCGAGTACGCCAAGTCCGGCAAGTCCTCGTGCAAGAAGTGCTCGGAACCGATCGAGAAGAAAGCTCTGAGGCTGGGCACCAAAGACGCGCGTGGTTATACCAAGTGGCACCACTTGTGCTGCTTCAGTTTTGGGTCCGACCCGGTTGCTTCGGTTGACAAGATTAAAGGGTTTCAGTCTCTCAAGAGTAGTGATCAGGAAGCTTTGAAGGATGAATTTGAGAAATCTAAAGAGGAGGATTCAAATGGAAAGAAAAGTTCTAAAAATCGAAAGGTTCGTGAAGTAGATCATGAAGCTGAAAATGAAAATAAAGGGGAAAGGAAGTTGAAGAAATCAAAGGATTCTTCTTCGTCCCATGTCGAAGCCGAGTACGCCAAGTCCAGCAAGTCCTCGTGCAACAAGTGCTCGAAACCTATCGAGAAGAAAGCTCTGAGGCTGGGCACCAAAGACGCGCGTGGTTATACCAAGTGGCACCACTTGGGTTGCTTCAGTTTTGGGTCCGACCCGGTTGTTTCGGTTGACAAGATCAAAGGATTTCAGTCTCTGGAGAGTAGTGATCAGGAAGCTTTGAAGAATTTGGTGGATGAATTTGAGAAATCTAAAGAGAGCTTTCCTCTAAGTGGGGTTTTTTTCACTATCGCACTAGGTGATACAACAAGAGACTGTGTACCTTCGGGAGACTGTCTTTTCGGTCCTACCCAGGATCATACCTTCATTCAGTTTATCCGAACTCATTTCACTCGGTTGCTGTGTCTCTGGTTCTTTCGGCAGAGTAGGTTCTTGATGCTTGTGATGATTACGGTGATTTCTGGAGGTTTGAAGGCTGGTTTTATCGAATTTGGCATAGGTCTTACTCGATCCATTGCAATGGAAGGCTATTACCACTGTTTGCTTCATTATGGAATGGAGGCTCTGTCTTTCACGACGGTGTGGTCGCTGGTCGCGGCGTGGCATAGCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

335

Amino Acids

37.37

Weight (kDa)

8.81

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-PARP PF00645 17 - 87 4.9e-16 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
zf-PARP PF00645 138 - 208 8.8e-16 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 976
AccB1I GGYRCC 4 cut(s) 122, 153, 485, 516
AccII CGCG 5 cut(s) 134, 136, 497, 499, 989
AciI CCGC 2 cut(s) 33, 989
AclWI GGATC 2 cut(s) 756, 897
AcoI YGGCCR 1 cut(s) 30
AcsI RAATTY 4 cut(s) 260, 619, 632, 881
AcuI CTGAAG 2 cut(s) 154, 517
AdeI CACNNNGTG 1 cut(s) 692
AfaI GTAC 3 cut(s) 53, 416, 714
AfiI CCNNNNNNNGG 1 cut(s) 665
AgsI TTSAA 5 cut(s) 253, 288, 373, 616, 865
AjnI CCWGG 1 cut(s) 744
AleI CACNNNNGTG 1 cut(s) 971
AluBI AGCT 7 cut(s) 110, 248, 341, 473, 611, 654, 1002
AluI AGCT 7 cut(s) 110, 248, 341, 473, 611, 654, 1002
Alw21I GWGCWC 2 cut(s) 89, 452
Alw26I GTCTC 5 cut(s) 228, 591, 699, 717, 803
AlwI GGATC 2 cut(s) 756, 897
AlwNI CAGNNNCTG 1 cut(s) 589
AoxI GGCC 1 cut(s) 30
ApeKI GCWGC 1 cut(s) 165
ApoI RAATTY 4 cut(s) 260, 619, 632, 881
ArsI GACNNNNNNTTYG 2 cut(s) 716, 748
AspLEI GCGC 2 cut(s) 136, 499
AspS9I GGNCC 3 cut(s) 180, 543, 737
AsuC2I CCSGG 2 cut(s) 188, 551
AsuHPI GGTGA 2 cut(s) 704, 859
AvaII GGWCC 3 cut(s) 180, 543, 737
BaeGI GKGCMC 2 cut(s) 125, 488
BanI GGYRCC 4 cut(s) 122, 153, 485, 516
BauI CACGAG 2 cut(s) 73, 436
Bbv12I GWGCWC 2 cut(s) 89, 452
BbvI GCAGC 1 cut(s) 152
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 1 cut(s) 746
BclI TGATCA 2 cut(s) 238, 601
BcnI CCSGG 2 cut(s) 188, 551
BcoDI GTCTC 5 cut(s) 228, 591, 699, 717, 803
BfaI CTAG 1 cut(s) 689
BisI GCNGC 3 cut(s) 33, 166, 990
BlsI GCNGC 3 cut(s) 34, 167, 991
Bme1390I CCNGG 3 cut(s) 188, 551, 746
Bme18I GGWCC 3 cut(s) 180, 543, 737
BmgT120I GGNCC 3 cut(s) 180, 543, 737
BmiI GGNNCC 7 cut(s) 93, 124, 155, 181, 487, 518, 544
BmrFI CCNGG 3 cut(s) 188, 551, 746
BmsI GCATC 1 cut(s) 819
BplI GAGNNNNNCTC 2 cut(s) 643, 675
BpmI CTGGAG 2 cut(s) 611, 876
BpuEI CTTGAG 2 cut(s) 9, 212
BpuMI CCSGG 2 cut(s) 188, 551
BsaJI CCNNGG 1 cut(s) 744
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 1 cut(s) 665
Bse3DI GCAATG 2 cut(s) 906, 918
BseBI CCWGG 1 cut(s) 746
BseDI CCNNGG 1 cut(s) 744
BseGI GGATG 2 cut(s) 262, 634
BseLI CCNNNNNNNGG 1 cut(s) 665
BseMI GCAATG 2 cut(s) 906, 918
BseMII CTCAG 2 cut(s) 104, 467
BseRI GAGGAG 1 cut(s) 293
BseSI GKGCMC 2 cut(s) 125, 488
BseXI GCAGC 1 cut(s) 152
BseYI CCCAGC 2 cut(s) 118, 481
Bsh1236I CGCG 5 cut(s) 134, 136, 497, 499, 989
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 4 cut(s) 122, 153, 485, 516
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 2 cut(s) 89, 452
BsiSI CCGG 3 cut(s) 63, 188, 551
BslFI GGGAC 1 cut(s) 382
BslI CCNNNNNNNGG 1 cut(s) 665
BsmAI GTCTC 5 cut(s) 228, 591, 699, 717, 803
BsmFI GGGAC 1 cut(s) 382
BsnI GGCC 1 cut(s) 32
Bsp1286I GDGCHC 4 cut(s) 89, 125, 452, 488
Bsp143I GATC 7 cut(s) 96, 238, 331, 570, 601, 748, 902
BspACI CCGC 2 cut(s) 33, 989
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 2 cut(s) 105, 468
BspFNI CGCG 5 cut(s) 134, 136, 497, 499, 989
BspHI TCATGA 1 cut(s) 334
BspLI GGNNCC 7 cut(s) 93, 124, 155, 181, 487, 518, 544
BspPI GGATC 2 cut(s) 756, 897
BspT107I GGYRCC 4 cut(s) 122, 153, 485, 516
BsrDI GCAATG 2 cut(s) 906, 918
BssECI CCNNGG 1 cut(s) 744
BssMI GATC 7 cut(s) 96, 238, 331, 570, 601, 748, 902
BssSI CACGAG 2 cut(s) 73, 436
Bst2BI CACGAG 2 cut(s) 73, 436
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 5 cut(s) 710, 728, 847, 932, 973
BstAPI GCANNNNNTGC 2 cut(s) 84, 447
BstDEI CTNAG 3 cut(s) 113, 476, 662
BstF5I GGATG 2 cut(s) 262, 634
BstFNI CGCG 5 cut(s) 134, 136, 497, 499, 989
BstHHI GCGC 2 cut(s) 136, 499
BstKTI GATC 7 cut(s) 99, 241, 334, 573, 604, 751, 905
BstMAI GTCTC 5 cut(s) 228, 591, 699, 717, 803
BstMBI GATC 7 cut(s) 96, 238, 331, 570, 601, 748, 902
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 4 cut(s) 53, 84, 416, 447
BstNI CCWGG 1 cut(s) 746
BstSCI CCNGG 3 cut(s) 186, 549, 744
BstSLI GKGCMC 2 cut(s) 125, 488
BstUI CGCG 5 cut(s) 134, 136, 497, 499, 989
BstV1I GCAGC 1 cut(s) 152
BstXI CCANNNNNNTGG 1 cut(s) 913
BsuRI GGCC 1 cut(s) 32
BtsCI GGATG 2 cut(s) 262, 634
BtsIMutI CAGTG 1 cut(s) 928
CaiI CAGNNNCTG 1 cut(s) 589
CciI TCATGA 1 cut(s) 334
CfoI GCGC 2 cut(s) 136, 499
Cfr13I GGNCC 3 cut(s) 180, 543, 737
CseI GACGC 2 cut(s) 140, 503
Csp6I GTAC 3 cut(s) 52, 415, 713
CspCI CAANNNNNGTGG 2 cut(s) 509, 544
CviAII CATG 2 cut(s) 335, 401
CviQI GTAC 3 cut(s) 52, 415, 713
DdeI CTNAG 3 cut(s) 113, 476, 662
DpnI GATC 7 cut(s) 98, 240, 333, 572, 603, 750, 904
DpnII GATC 7 cut(s) 96, 238, 331, 570, 601, 748, 902
DraIII CACNNNGTG 1 cut(s) 692
DrdI GACNNNNNNGTC 1 cut(s) 976
DseDI GACNNNNNNGTC 1 cut(s) 976
EaeI YGGCCR 1 cut(s) 30
Eco47I GGWCC 3 cut(s) 180, 543, 737
Eco57I CTGAAG 2 cut(s) 154, 517
EcoRII CCWGG 1 cut(s) 744
FaeI CATG 2 cut(s) 338, 404
FaiI YATR 9 cut(s) 144, 336, 402, 507, 753, 890, 945, 999, 1006
FaqI GGGAC 1 cut(s) 382
FatI CATG 2 cut(s) 334, 400
FbaI TGATCA 2 cut(s) 238, 601
Fnu4HI GCNGC 3 cut(s) 33, 166, 990
FokI GGATG 2 cut(s) 269, 641
Fsp4HI GCNGC 3 cut(s) 33, 166, 990
FspBI CTAG 1 cut(s) 689
GlaI GCGC 2 cut(s) 135, 498
GluI GCNGC 3 cut(s) 33, 166, 990
GsaI CCCAGC 2 cut(s) 122, 485
GsuI CTGGAG 2 cut(s) 611, 876
HaeIII GGCC 1 cut(s) 32
HapII CCGG 3 cut(s) 63, 188, 551
HgaI GACGC 2 cut(s) 140, 503
HhaI GCGC 2 cut(s) 136, 499
Hin1II CATG 2 cut(s) 338, 404
Hin6I GCGC 2 cut(s) 134, 497
HinP1I GCGC 2 cut(s) 134, 497
HincII GTYRAC 2 cut(s) 202, 565
HindII GTYRAC 2 cut(s) 202, 565
HindIII AAGCTT 2 cut(s) 246, 609
HinfI GANTC 3 cut(s) 22, 284, 386
HpaII CCGG 3 cut(s) 63, 188, 551
HphI GGTGA 2 cut(s) 704, 859
Hpy166II GTNNAC 3 cut(s) 202, 565, 713
Hpy188I TCNGA 6 cut(s) 91, 114, 184, 477, 547, 773
Hpy8I GTNNAC 3 cut(s) 202, 565, 713
Hpy99I CGWCG 1 cut(s) 973
HpyAV CCTTC 5 cut(s) 247, 726, 766, 859, 911
HpyCH4III ACNGT 5 cut(s) 710, 728, 847, 932, 973
HpyCH4V TGCA 3 cut(s) 78, 441, 911
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 84, 416, 447
HpyF3I CTNAG 3 cut(s) 113, 476, 662
Hsp92II CATG 2 cut(s) 338, 404
HspAI GCGC 2 cut(s) 134, 497
Ksp22I TGATCA 2 cut(s) 238, 601
Kzo9I GATC 7 cut(s) 96, 238, 331, 570, 601, 748, 902
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 1 cut(s) 819
MaeI CTAG 1 cut(s) 689
MalI GATC 7 cut(s) 98, 240, 333, 572, 603, 750, 904
MboI GATC 7 cut(s) 96, 238, 331, 570, 601, 748, 902
MboII GAAGA 9 cut(s) 3, 6, 9, 115, 381, 384, 385, 478, 628
MhlI GDGCHC 4 cut(s) 89, 125, 452, 488
MluCI AATT 4 cut(s) 260, 619, 632, 881
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 2 cut(s) 207, 570
MnlI CCTC 9 cut(s) 82, 108, 271, 274, 445, 471, 669, 851, 946
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 2 cut(s) 911, 971
MspI CCGG 3 cut(s) 63, 188, 551
MspR9I CCNGG 3 cut(s) 188, 551, 746
MvaI CCWGG 1 cut(s) 746
MvnI CGCG 5 cut(s) 134, 136, 497, 499, 989
MwoI GCNNNNNNNGC 4 cut(s) 53, 84, 416, 447
NciI CCSGG 2 cut(s) 188, 551
NdeII GATC 7 cut(s) 96, 238, 331, 570, 601, 748, 902
NlaIII CATG 2 cut(s) 338, 404
NlaIV GGNNCC 7 cut(s) 93, 124, 155, 181, 487, 518, 544
NmeAIII GCCGAG 2 cut(s) 73, 436
OliI CACNNNNGTG 1 cut(s) 971
PagI TCATGA 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 319
PfeI GAWTC 2 cut(s) 284, 386
PkrI GCNGC 3 cut(s) 34, 167, 991
Ple19I CGATCG 1 cut(s) 99
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Psp6I CCWGG 1 cut(s) 744
PspFI CCCAGC 2 cut(s) 118, 481
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 7 cut(s) 93, 124, 155, 181, 487, 518, 544
PspPI GGNCC 3 cut(s) 180, 543, 737
PstNI CAGNNNCTG 1 cut(s) 589
PvuI CGATCG 1 cut(s) 99
RsaI GTAC 3 cut(s) 53, 416, 714
RsaNI GTAC 3 cut(s) 52, 415, 713
RseI CAYNNNNRTG 2 cut(s) 911, 971
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 3 cut(s) 33, 166, 990
Sau3AI GATC 7 cut(s) 96, 238, 331, 570, 601, 748, 902
Sau96I GGNCC 3 cut(s) 180, 543, 737
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 3 cut(s) 188, 551, 746
SduI GDGCHC 4 cut(s) 89, 125, 452, 488
SfaNI GCATC 1 cut(s) 819
SinI GGWCC 3 cut(s) 180, 543, 737
SmiMI CAYNNNNRTG 2 cut(s) 911, 971
SmlI CTYRAG 2 cut(s) 24, 227
SmoI CTYRAG 2 cut(s) 24, 227
Sse9I AATT 4 cut(s) 260, 619, 632, 881
SsiI CCGC 2 cut(s) 33, 989
SspMI CTAG 1 cut(s) 689
StyD4I CCNGG 3 cut(s) 186, 549, 744
TaaI ACNGT 5 cut(s) 710, 728, 847, 932, 973
TaqI TCGA 9 cut(s) 20, 42, 99, 313, 405, 452, 462, 879, 901
TaqII GACCGA 1 cut(s) 725
TasI AATT 4 cut(s) 260, 619, 632, 881
TauI GCSGC 2 cut(s) 35, 992
TfiI GAWTC 2 cut(s) 284, 386
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 1 cut(s) 935
TseI GCWGC 1 cut(s) 165
TspDTI ATGAA 6 cut(s) 273, 351, 363, 645, 748, 929
TspRI CASTG 1 cut(s) 935
VpaK11BI GGWCC 3 cut(s) 180, 543, 737
XapI RAATTY 4 cut(s) 260, 619, 632, 881
XspI CTAG 1 cut(s) 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.