Rh5AG214300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000926
Physical Location & Seq
Reverse (-)
25231418 .. 25232260
843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG214300.1

Sequence Viewer

Length: 843 bp
ATGGATCCCTATTATGAACAAAGACTCAGAGACGAAGTCATTTACCTCCACTCTCTCTGGCACCAAGGTCCCCCTAATCGCCGAAACCCCATTCCCAATTCCACAATCCGAGCCACGCCAAATGGCGGAAAACCCCAATTGCCGTACCCGATCGCCGCCCAAGAAGGCCCCACCACCAAGAGGAAGACAAGAACCGAAATTGAATTGCCCAGCAGCAACAAGAAGCCCTCGCAACCCGACCCGCGTTGGGAATGCCCTGGTCCGGCCTCGAACGATGACTCAGCCTCTGAGTGGCCATCATCTGGGTGGCCAGTGACTTCAGCCAAGTTCAATTTGGCTCCGGCGTCGGCGGAAGAGCAGCTGAGGCACCACGCGCTTCGGCTACACAACCGGGCCTTGGAGGCCTGCGGCGGATTCTTTAAGGGCAATGCCGGGTCGGGTAGTGGGTCCGAGAGTGAGGAGGAAGAAGAGGATTGCAAGGTTGGAGATGAGGAGAATGAGGAGTACAAGTTTTTGTTGAATTTGTTTGTGGAGGAGAGTGGGTTGAGGAGTTATTATGAGGGGAATTGGGATAGTGGTGAGTTCCATTGCTTGGTGTGTGGTGGGTTGGGGAAGACGAAGAAGATCAAGGGTTGTGTCGGGCTTGTTCACCACGCAGTTTCGATAAGCAAGACGAAGAAGAAGAGAGCTCATAGGGCTTTTGCTCAGGTTGTGTGTAGGGTTTTAGGCTGGGATTTTGACCGGCTGCCCATTGTTGTGCTCAAAGGCGAAAGGCTCAGTTTTTATTTGGCAAAACCGGGCGATGAACGGGTGATTAGAGATTGTTTTTCGGGTAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

31.28

Weight (kDa)

7.11

Isoelectric Point (pI)

54.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 60, 366
AccB7I CCANNNNNTGG 2 cut(s) 302, 592
AccII CGCG 2 cut(s) 244, 374
AciI CCGC 6 cut(s) 126, 156, 242, 350, 408, 411
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 293, 308
AcsI RAATTY 1 cut(s) 520
AcuI CTGAAG 1 cut(s) 303
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 2 cut(s) 146, 506
AfiI CCNNNNNNNGG 8 cut(s) 125, 180, 247, 262, 291, 302, 397, 592
AgsI TTSAA 3 cut(s) 203, 331, 520
AjnI CCWGG 1 cut(s) 256
AluBI AGCT 2 cut(s) 361, 689
AluI AGCT 2 cut(s) 361, 689
Alw21I GWGCWC 2 cut(s) 691, 762
Alw26I GTCTC 1 cut(s) 24
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 1 cut(s) 287
AoxI GGCC 6 cut(s) 166, 264, 293, 308, 393, 402
ApeKI GCWGC 3 cut(s) 213, 358, 745
ApoI RAATTY 1 cut(s) 520
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 5 cut(s) 68, 167, 260, 393, 447
AsuC2I CCSGG 3 cut(s) 392, 433, 798
AsuHPI GGTGA 3 cut(s) 590, 641, 823
AvaII GGWCC 3 cut(s) 68, 260, 447
BalI TGGCCA 2 cut(s) 295, 310
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 60, 366
BanII GRGCYC 1 cut(s) 691
BbsI GAAGAC 2 cut(s) 191, 620
Bbv12I GWGCWC 2 cut(s) 691, 762
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 3 cut(s) 225, 370, 732
BccI CCATC 1 cut(s) 304
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 1 cut(s) 258
BcnI CCSGG 3 cut(s) 392, 433, 798
BcoDI GTCTC 1 cut(s) 24
BglI GCCNNNNNGGC 1 cut(s) 401
BisI GCNGC 5 cut(s) 156, 214, 359, 409, 746
BlsI GCNGC 5 cut(s) 157, 215, 360, 410, 747
Bme1390I CCNGG 4 cut(s) 258, 392, 433, 798
Bme18I GGWCC 3 cut(s) 68, 260, 447
BmgT120I GGNCC 5 cut(s) 68, 167, 260, 393, 447
BmiI GGNNCC 7 cut(s) 6, 62, 70, 169, 339, 368, 448
BmrFI CCNGG 4 cut(s) 258, 392, 433, 798
BpiI GAAGAC 2 cut(s) 191, 620
Bpu10I CCTNAGC 2 cut(s) 362, 705
BpuMI CCSGG 3 cut(s) 392, 433, 798
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 3 cut(s) 64, 256, 396
BsaXI ACNNNNNCTCC 4 cut(s) 494, 524, 527, 557
Bsc4I CCNNNNNNNGG 8 cut(s) 125, 180, 247, 262, 291, 302, 397, 592
Bse118I RCCGGY 1 cut(s) 741
Bse1I ACTGG 1 cut(s) 311
Bse3DI GCAATG 2 cut(s) 433, 586
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 64, 256, 396
BseLI CCNNNNNNNGG 8 cut(s) 125, 180, 247, 262, 291, 302, 397, 592
BseMI GCAATG 2 cut(s) 433, 586
BseMII CTCAG 6 cut(s) 40, 279, 294, 353, 719, 790
BseNI ACTGG 1 cut(s) 311
BseRI GAGGAG 5 cut(s) 473, 506, 515, 548, 562
BseXI GCAGC 3 cut(s) 225, 370, 732
BseYI CCCAGC 2 cut(s) 209, 729
Bsh1236I CGCG 2 cut(s) 244, 374
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 6 cut(s) 168, 266, 295, 310, 395, 404
BshNI GGYRCC 2 cut(s) 60, 366
BsiEI CGRYCG 1 cut(s) 153
BsiHKAI GWGCWC 2 cut(s) 691, 762
BsiSI CCGG 6 cut(s) 263, 341, 391, 432, 742, 797
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 8 cut(s) 125, 180, 247, 262, 291, 302, 397, 592
BsmAI GTCTC 1 cut(s) 24
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 54
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 6 cut(s) 168, 266, 295, 310, 395, 404
Bsp1286I GDGCHC 2 cut(s) 691, 762
Bsp143I GATC 3 cut(s) 4, 150, 624
BspACI CCGC 6 cut(s) 126, 156, 242, 350, 408, 411
BspANI GGCC 6 cut(s) 168, 266, 295, 310, 395, 404
BspCNI CTCAG 6 cut(s) 39, 280, 293, 354, 718, 789
BspFNI CGCG 2 cut(s) 244, 374
BspLI GGNNCC 7 cut(s) 6, 62, 70, 169, 339, 368, 448
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 348
BspT107I GGYRCC 2 cut(s) 60, 366
BsrDI GCAATG 2 cut(s) 433, 586
BsrFI RCCGGY 1 cut(s) 741
BsrI ACTGG 1 cut(s) 311
BssAI RCCGGY 1 cut(s) 741
BssECI CCNNGG 3 cut(s) 64, 256, 396
BssMI GATC 3 cut(s) 4, 150, 624
BssNI GRCGYC 1 cut(s) 344
BssT1I CCWWGG 2 cut(s) 64, 396
Bst2UI CCWGG 1 cut(s) 258
Bst6I CTCTTC 3 cut(s) 348, 462, 677
BstACI GRCGYC 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 406
BstDEI CTNAG 6 cut(s) 26, 280, 288, 362, 705, 776
BstFNI CGCG 2 cut(s) 244, 374
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 3 cut(s) 7, 153, 627
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 3 cut(s) 4, 150, 624
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 4 cut(s) 364, 373, 401, 695
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 4 cut(s) 256, 390, 431, 796
BstUI CGCG 2 cut(s) 244, 374
BstV1I GCAGC 3 cut(s) 225, 370, 732
BstV2I GAAGAC 2 cut(s) 191, 620
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 6 cut(s) 168, 266, 295, 310, 395, 404
BtgZI GCGATG 1 cut(s) 816
BtsIMutI CAGTG 1 cut(s) 318
Cac8I GCNNGC 1 cut(s) 406
CaiI CAGNNNCTG 1 cut(s) 287
CfoI GCGC 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 741
Cfr13I GGNCC 5 cut(s) 68, 167, 260, 393, 447
CseI GACGC 1 cut(s) 333
Csp6I GTAC 2 cut(s) 145, 505
CviQI GTAC 2 cut(s) 145, 505
DdeI CTNAG 6 cut(s) 26, 280, 288, 362, 705, 776
DpnI GATC 3 cut(s) 6, 152, 626
DpnII GATC 3 cut(s) 4, 150, 624
EaeI YGGCCR 2 cut(s) 293, 308
Eam1104I CTCTTC 3 cut(s) 348, 462, 677
EarI CTCTTC 3 cut(s) 348, 462, 677
EciI GGCGGA 3 cut(s) 141, 365, 426
Ecl136II GAGCTC 1 cut(s) 689
Eco130I CCWWGG 2 cut(s) 64, 396
Eco147I AGGCCT 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 691
Eco47I GGWCC 3 cut(s) 68, 260, 447
Eco53kI GAGCTC 1 cut(s) 689
Eco57I CTGAAG 1 cut(s) 303
EcoICRI GAGCTC 1 cut(s) 689
EcoO109I RGGNCCY 2 cut(s) 68, 167
EcoRII CCWGG 1 cut(s) 256
EcoT14I CCWWGG 2 cut(s) 64, 396
EcoT38I GRGCYC 1 cut(s) 691
ErhI CCWWGG 2 cut(s) 64, 396
Esp3I CGTCTC 1 cut(s) 24
FaiI YATR 3 cut(s) 15, 558, 693
FaqI GGGAC 1 cut(s) 54
FauI CCCGC 1 cut(s) 249
Fnu4HI GCNGC 5 cut(s) 156, 214, 359, 409, 746
FriOI GRGCYC 1 cut(s) 691
Fsp4HI GCNGC 5 cut(s) 156, 214, 359, 409, 746
GlaI GCGC 1 cut(s) 375
GluI GCNGC 5 cut(s) 156, 214, 359, 409, 746
GsaI CCCAGC 2 cut(s) 213, 733
HaeIII GGCC 6 cut(s) 168, 266, 295, 310, 395, 404
HapII CCGG 6 cut(s) 263, 341, 391, 432, 742, 797
HgaI GACGC 1 cut(s) 333
HhaI GCGC 1 cut(s) 376
Hin1I GRCGYC 1 cut(s) 344
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HinfI GANTC 3 cut(s) 24, 278, 414
HpaII CCGG 6 cut(s) 263, 341, 391, 432, 742, 797
HphI GGTGA 3 cut(s) 590, 641, 823
Hpy166II GTNNAC 1 cut(s) 649
Hpy188I TCNGA 4 cut(s) 29, 110, 289, 451
Hpy8I GTNNAC 1 cut(s) 649
Hpy99I CGWCG 1 cut(s) 349
HpyAV CCTTC 1 cut(s) 158
HpyCH4V TGCA 1 cut(s) 477
HpyF10VI GCNNNNNNNGC 4 cut(s) 364, 373, 401, 695
HpyF3I CTNAG 6 cut(s) 26, 280, 288, 362, 705, 776
Hsp92I GRCGYC 1 cut(s) 344
HspAI GCGC 1 cut(s) 374
Kzo9I GATC 3 cut(s) 4, 150, 624
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 1 cut(s) 343
Lsp1109I GCAGC 3 cut(s) 225, 370, 732
MaeIII GTNAC 1 cut(s) 313
MalI GATC 3 cut(s) 6, 152, 626
MboI GATC 3 cut(s) 4, 150, 624
MfeI CAATTG 1 cut(s) 137
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 691, 762
MlsI TGGCCA 2 cut(s) 295, 310
MluCI AATT 7 cut(s) 97, 137, 198, 203, 331, 520, 565
MluNI TGGCCA 2 cut(s) 295, 310
MlyI GAGTC 2 cut(s) 18, 272
MmeI TCCRAC 1 cut(s) 463
Mox20I TGGCCA 2 cut(s) 295, 310
MscI TGGCCA 2 cut(s) 295, 310
MseI TTAA 1 cut(s) 420
MslI CAYNNNNRTG 2 cut(s) 304, 755
Msp20I TGGCCA 2 cut(s) 295, 310
MspA1I CMGCKG 1 cut(s) 361
MspI CCGG 6 cut(s) 263, 341, 391, 432, 742, 797
MspR9I CCNGG 4 cut(s) 258, 392, 433, 798
MunI CAATTG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 257
MvaI CCWGG 1 cut(s) 258
MvnI CGCG 2 cut(s) 244, 374
MwoI GCNNNNNNNGC 4 cut(s) 364, 373, 401, 695
NciI CCSGG 3 cut(s) 392, 433, 798
NdeII GATC 3 cut(s) 4, 150, 624
NlaIV GGNNCC 7 cut(s) 6, 62, 70, 169, 339, 368, 448
NmuCI GTSAC 1 cut(s) 313
PceI AGGCCT 1 cut(s) 404
PciSI GCTCTTC 1 cut(s) 348
PctI GAATGC 1 cut(s) 257
PfeI GAWTC 1 cut(s) 414
PflFI GACNNNGTC 1 cut(s) 35
PflMI CCANNNNNTGG 2 cut(s) 302, 592
PkrI GCNGC 5 cut(s) 157, 215, 360, 410, 747
Ple19I CGATCG 1 cut(s) 153
PleI GAGTC 2 cut(s) 18, 272
PpsI GAGTC 2 cut(s) 18, 272
PpuMI RGGWCCY 1 cut(s) 68
Psp124BI GAGCTC 1 cut(s) 691
Psp5II RGGWCCY 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 256
PspFI CCCAGC 2 cut(s) 209, 729
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 7 cut(s) 6, 62, 70, 169, 339, 368, 448
PspPI GGNCC 5 cut(s) 68, 167, 260, 393, 447
PspPPI RGGWCCY 1 cut(s) 68
PstNI CAGNNNCTG 1 cut(s) 287
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 35
PvuI CGATCG 1 cut(s) 153
PvuII CAGCTG 1 cut(s) 361
RsaI GTAC 2 cut(s) 146, 506
RsaNI GTAC 2 cut(s) 145, 505
RseI CAYNNNNRTG 2 cut(s) 304, 755
SacI GAGCTC 1 cut(s) 691
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 5 cut(s) 156, 214, 359, 409, 746
Sau3AI GATC 3 cut(s) 4, 150, 624
Sau96I GGNCC 5 cut(s) 68, 167, 260, 393, 447
SchI GAGTC 2 cut(s) 18, 272
ScrFI CCNGG 4 cut(s) 258, 392, 433, 798
SduI GDGCHC 2 cut(s) 691, 762
SetI ASST 6 cut(s) 48, 70, 363, 483, 691, 711
SfiI GGCCNNNNNGGCC 1 cut(s) 401
SinI GGWCC 3 cut(s) 68, 260, 447
SmiMI CAYNNNNRTG 2 cut(s) 304, 755
Sse9I AATT 7 cut(s) 97, 137, 198, 203, 331, 520, 565
SseBI AGGCCT 1 cut(s) 404
SsiI CCGC 6 cut(s) 126, 156, 242, 350, 408, 411
SstI GAGCTC 1 cut(s) 691
StuI AGGCCT 1 cut(s) 404
StyD4I CCNGG 4 cut(s) 256, 390, 431, 796
StyI CCWWGG 2 cut(s) 64, 396
TaqI TCGA 2 cut(s) 269, 662
TasI AATT 7 cut(s) 97, 137, 198, 203, 331, 520, 565
TatI WGTACW 1 cut(s) 504
TauI GCSGC 2 cut(s) 158, 411
TfiI GAWTC 1 cut(s) 414
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 1 cut(s) 318
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 3 cut(s) 213, 358, 745
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 2 cut(s) 30, 819
TspRI CASTG 1 cut(s) 318
Tth111I GACNNNGTC 1 cut(s) 35
Van91I CCANNNNNTGG 2 cut(s) 302, 592
VpaK11BI GGWCC 3 cut(s) 68, 260, 447
XapI RAATTY 1 cut(s) 520
XcmI CCANNNNNNNNNTGG 2 cut(s) 303, 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.