Rmu_co8456075.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8456075.1
OG0000926
Physical Location & Seq
Forward (+)
185 .. 1761
1577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8456075.1_g000001.1.cds

Sequence Viewer

Length: 1209 bp
atggatccctattatgaacaaagactcagagacgaagtcatttacctccactctctctggcaccaaggtccccctaatcgccgaaaccccattcccaattccacaatccgagccacgccaaatggcggaaaaccccaattgccgtacccgatcgccgcccaagaaggccccaccaccaagaggaagacaagaaccgaaattgaattgcccagcagcaacaagaagccctcgcaacccgacccgcgttgggaatgcccgggtccggccttgaacgatgactcaacctctgagtggccctcatctgggtggccagtgacttcagccaagcaaaatttggctacggcgtcggcggaagagcagctgaggcaccacgcgcttcggctacacaaccgggccttggaggcctgcggcggattctttaagggcaatgccgggtcgggtagtgggtccgagagtgaggaggaagaagagggttgcaaggttggagatgaggagaatgaggagtacaagtttttgttgaatttgtttgtggaggagagtgggttgaggagttattatgaggggaattgggatagtggtgagttccattgcttggtgtgtggtgggttggggaagacgaagaagatcaagggttgtgtcgggcttgttcaccacgcggtttcgataagcaagacgaagaagaagagagctcatagggcttttgctcaggttgtgtgtagggttttaggctgggattttgaccggctgcccattgttgtgctcaaaggcgaaaggctcagtttttatttggcaaaaccgggcgatgaacggggtgagagtgaggtaaatgctggtgctggtgaggctgtttcgggtgctgttaaagacaatgtgggagatgaaagggatggtaatgccgagtcaggtgaaaaggaagcatctaatggagacctaatgatttgtcagaaatctttgaaagatgatgatgcaaatcaatgcaaagaaacccttaataaggggatgtcagaaatcaaagaagagaaagaagaggcaatggatacgcggagtgagcctgagttaaatgttgaccggttggatagtgcactgacagacaatgtggcagctgaaactgatgctcctgctgaatctagtgaaaaggaggcatttgatgatcatcagtgcaatggtaaagaaccaatcatctgtcaggttagtcatcactgtaactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

402

Amino Acids

44.28

Weight (kDa)

5.14

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 60, 366
AccB7I CCANNNNNTGG 1 cut(s) 592
AccII CGCG 4 cut(s) 244, 374, 656, 1042
AciI CCGC 8 cut(s) 126, 156, 242, 350, 408, 411, 656, 1042
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 308
AcsI RAATTY 2 cut(s) 331, 520
AcuI CTGAAG 1 cut(s) 303
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 2 cut(s) 146, 506
AgeI ACCGGT 1 cut(s) 1068
AgsI TTSAA 4 cut(s) 203, 271, 520, 955
AluBI AGCT 3 cut(s) 361, 689, 1103
AluI AGCT 3 cut(s) 361, 689, 1103
Alw21I GWGCWC 3 cut(s) 691, 762, 1084
Alw26I GTCTC 2 cut(s) 24, 921
Alw44I GTGCAC 1 cut(s) 1080
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 6 cut(s) 166, 264, 293, 308, 393, 402
ApaLI GTGCAC 1 cut(s) 1080
ApeKI GCWGC 4 cut(s) 213, 358, 745, 1100
ApoI RAATTY 2 cut(s) 331, 520
AsiGI ACCGGT 1 cut(s) 1068
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 6 cut(s) 68, 167, 260, 294, 393, 447
AsuC2I CCSGG 5 cut(s) 257, 258, 392, 433, 798
AsuHPI GGTGA 5 cut(s) 590, 641, 824, 851, 917
AvaI CYCGRG 1 cut(s) 256
AvaII GGWCC 3 cut(s) 68, 260, 447
BaeGI GKGCMC 1 cut(s) 1084
BalI TGGCCA 1 cut(s) 310
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 60, 366
BanII GRGCYC 1 cut(s) 691
BbsI GAAGAC 2 cut(s) 191, 620
Bbv12I GWGCWC 3 cut(s) 691, 762, 1084
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 4 cut(s) 225, 370, 732, 1112
BccI CCATC 1 cut(s) 881
BceAI ACGGC 2 cut(s) 127, 357
BciVI GTATCC 1 cut(s) 1030
BclI TGATCA 1 cut(s) 1150
BcnI CCSGG 5 cut(s) 257, 258, 392, 433, 798
BcoDI GTCTC 2 cut(s) 24, 921
BfaI CTAG 2 cut(s) 1128, 1207
BfuI GTATCC 1 cut(s) 1030
BglI GCCNNNNNGGC 1 cut(s) 401
BisI GCNGC 6 cut(s) 156, 214, 359, 409, 746, 1101
BlsI GCNGC 6 cut(s) 157, 215, 360, 410, 747, 1102
Bme1390I CCNGG 5 cut(s) 257, 258, 392, 433, 798
Bme18I GGWCC 3 cut(s) 68, 260, 447
BmeT110I CYCGRG 1 cut(s) 256
BmgT120I GGNCC 6 cut(s) 68, 167, 260, 294, 393, 447
BmiI GGNNCC 7 cut(s) 6, 62, 70, 169, 261, 368, 448
BmrFI CCNGG 5 cut(s) 257, 258, 392, 433, 798
BmsI GCATC 3 cut(s) 926, 955, 1102
BpiI GAAGAC 2 cut(s) 191, 620
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu10I CCTNAGC 2 cut(s) 362, 705
BpuMI CCSGG 5 cut(s) 257, 258, 392, 433, 798
BsaBI GATNNNNATC 2 cut(s) 969, 1152
BsaHI GRCGYC 1 cut(s) 344
BsaI GGTCTC 1 cut(s) 921
BsaJI CCNNGG 3 cut(s) 64, 256, 396
BsaWI WCCGGW 1 cut(s) 1068
BsaXI ACNNNNNCTCC 6 cut(s) 494, 524, 527, 557, 1099, 1129
Bse118I RCCGGY 2 cut(s) 741, 1068
Bse1I ACTGG 1 cut(s) 311
Bse3DI GCAATG 4 cut(s) 433, 586, 1038, 1168
Bse8I GATNNNNATC 2 cut(s) 969, 1152
BseDI CCNNGG 3 cut(s) 64, 256, 396
BseGI GGATG 2 cut(s) 892, 1005
BseJI GATNNNNATC 2 cut(s) 969, 1152
BseMI GCAATG 4 cut(s) 433, 586, 1038, 1168
BseMII CTCAG 6 cut(s) 40, 279, 353, 719, 790, 1044
BseNI ACTGG 1 cut(s) 311
BseRI GAGGAG 5 cut(s) 473, 506, 515, 548, 562
BseSI GKGCMC 1 cut(s) 1084
BseXI GCAGC 4 cut(s) 225, 370, 732, 1112
BseYI CCCAGC 2 cut(s) 209, 729
Bsh1236I CGCG 4 cut(s) 244, 374, 656, 1042
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 6 cut(s) 168, 266, 295, 310, 395, 404
BshNI GGYRCC 2 cut(s) 60, 366
BshTI ACCGGT 1 cut(s) 1068
BsiEI CGRYCG 1 cut(s) 153
BsiHKAI GWGCWC 3 cut(s) 691, 762, 1084
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 7 cut(s) 257, 263, 391, 432, 742, 797, 1069
BslFI GGGAC 1 cut(s) 54
BsmAI GTCTC 2 cut(s) 24, 921
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 54
BsmI GAATGC 1 cut(s) 257
BsnI GGCC 6 cut(s) 168, 266, 295, 310, 395, 404
Bso31I GGTCTC 1 cut(s) 921
BsoBI CYCGRG 1 cut(s) 256
Bsp1286I GDGCHC 3 cut(s) 691, 762, 1084
Bsp143I GATC 4 cut(s) 4, 150, 624, 1150
BspACI CCGC 8 cut(s) 126, 156, 242, 350, 408, 411, 656, 1042
BspANI GGCC 6 cut(s) 168, 266, 295, 310, 395, 404
BspCNI CTCAG 6 cut(s) 39, 280, 354, 718, 789, 1045
BspFNI CGCG 4 cut(s) 244, 374, 656, 1042
BspLI GGNNCC 7 cut(s) 6, 62, 70, 169, 261, 368, 448
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 348
BspT107I GGYRCC 2 cut(s) 60, 366
BspTNI GGTCTC 1 cut(s) 921
BsrDI GCAATG 4 cut(s) 433, 586, 1038, 1168
BsrFI RCCGGY 2 cut(s) 741, 1068
BsrI ACTGG 1 cut(s) 311
BssAI RCCGGY 2 cut(s) 741, 1068
BssECI CCNNGG 3 cut(s) 64, 256, 396
BssMI GATC 4 cut(s) 4, 150, 624, 1150
BssNI GRCGYC 1 cut(s) 344
BssT1I CCWWGG 2 cut(s) 64, 396
Bst4CI ACNGT 1 cut(s) 1202
Bst6I CTCTTC 5 cut(s) 348, 462, 677, 1011, 1020
BstACI GRCGYC 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 406
BstDEI CTNAG 6 cut(s) 26, 288, 362, 705, 776, 1053
BstENI CCTNNNNNAGG 1 cut(s) 992
BstF5I GGATG 2 cut(s) 892, 1005
BstFNI CGCG 4 cut(s) 244, 374, 656, 1042
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 4 cut(s) 7, 153, 627, 1153
BstMAI GTCTC 2 cut(s) 24, 921
BstMBI GATC 4 cut(s) 4, 150, 624, 1150
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 6 cut(s) 364, 373, 401, 695, 842, 1048
BstSCI CCNGG 5 cut(s) 255, 256, 390, 431, 796
BstSLI GKGCMC 1 cut(s) 1084
BstUI CGCG 4 cut(s) 244, 374, 656, 1042
BstV1I GCAGC 4 cut(s) 225, 370, 732, 1112
BstV2I GAAGAC 2 cut(s) 191, 620
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 1 cut(s) 1030
BsuRI GGCC 6 cut(s) 168, 266, 295, 310, 395, 404
BtgZI GCGATG 1 cut(s) 816
BtsCI GGATG 2 cut(s) 892, 1005
BtsIMutI CAGTG 4 cut(s) 318, 1082, 1163, 1198
Cac8I GCNNGC 1 cut(s) 406
CfoI GCGC 1 cut(s) 376
Cfr10I RCCGGY 2 cut(s) 741, 1068
Cfr13I GGNCC 6 cut(s) 68, 167, 260, 294, 393, 447
Cfr9I CCCGGG 1 cut(s) 256
CseI GACGC 1 cut(s) 333
Csp6I GTAC 2 cut(s) 145, 505
CspAI ACCGGT 1 cut(s) 1068
CviQI GTAC 2 cut(s) 145, 505
DdeI CTNAG 6 cut(s) 26, 288, 362, 705, 776, 1053
DpnI GATC 4 cut(s) 6, 152, 626, 1152
DpnII GATC 4 cut(s) 4, 150, 624, 1150
EaeI YGGCCR 1 cut(s) 308
Eam1104I CTCTTC 5 cut(s) 348, 462, 677, 1011, 1020
EarI CTCTTC 5 cut(s) 348, 462, 677, 1011, 1020
EciI GGCGGA 3 cut(s) 141, 365, 426
Ecl136II GAGCTC 1 cut(s) 689
Eco130I CCWWGG 2 cut(s) 64, 396
Eco147I AGGCCT 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 691
Eco31I GGTCTC 1 cut(s) 921
Eco47I GGWCC 3 cut(s) 68, 260, 447
Eco53kI GAGCTC 1 cut(s) 689
Eco57I CTGAAG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 689
EcoNI CCTNNNNNAGG 1 cut(s) 992
EcoO109I RGGNCCY 2 cut(s) 68, 167
EcoT14I CCWWGG 2 cut(s) 64, 396
EcoT38I GRGCYC 1 cut(s) 691
ErhI CCWWGG 2 cut(s) 64, 396
Esp3I CGTCTC 1 cut(s) 24
FaiI YATR 3 cut(s) 15, 558, 693
FalI AAGNNNNNCTT 2 cut(s) 972, 1004
FaqI GGGAC 1 cut(s) 54
FauI CCCGC 1 cut(s) 249
FbaI TGATCA 1 cut(s) 1150
Fnu4HI GCNGC 6 cut(s) 156, 214, 359, 409, 746, 1101
FokI GGATG 2 cut(s) 899, 1012
FriOI GRGCYC 1 cut(s) 691
Fsp4HI GCNGC 6 cut(s) 156, 214, 359, 409, 746, 1101
FspBI CTAG 2 cut(s) 1128, 1207
GlaI GCGC 1 cut(s) 375
GluI GCNGC 6 cut(s) 156, 214, 359, 409, 746, 1101
GsaI CCCAGC 2 cut(s) 213, 733
HaeIII GGCC 6 cut(s) 168, 266, 295, 310, 395, 404
HapII CCGG 7 cut(s) 257, 263, 391, 432, 742, 797, 1069
HgaI GACGC 1 cut(s) 333
HhaI GCGC 1 cut(s) 376
Hin1I GRCGYC 1 cut(s) 344
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HincII GTYRAC 1 cut(s) 1066
HindII GTYRAC 1 cut(s) 1066
HinfI GANTC 5 cut(s) 24, 278, 414, 899, 1124
HpaII CCGG 7 cut(s) 257, 263, 391, 432, 742, 797, 1069
HphI GGTGA 5 cut(s) 590, 641, 824, 851, 917
Hpy166II GTNNAC 3 cut(s) 649, 1066, 1082
Hpy188I TCNGA 6 cut(s) 29, 110, 289, 451, 945, 1006
Hpy8I GTNNAC 3 cut(s) 649, 1066, 1082
Hpy99I CGWCG 1 cut(s) 349
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 1 cut(s) 1202
HpyCH4V TGCA 5 cut(s) 477, 968, 978, 1082, 1161
HpyF10VI GCNNNNNNNGC 6 cut(s) 364, 373, 401, 695, 842, 1048
HpyF3I CTNAG 6 cut(s) 26, 288, 362, 705, 776, 1053
Hsp92I GRCGYC 1 cut(s) 344
HspAI GCGC 1 cut(s) 374
Ksp22I TGATCA 1 cut(s) 1150
Kzo9I GATC 4 cut(s) 4, 150, 624, 1150
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 1 cut(s) 1120
Lsp1109I GCAGC 4 cut(s) 225, 370, 732, 1112
LweI GCATC 3 cut(s) 926, 955, 1102
MaeI CTAG 2 cut(s) 1128, 1207
MaeIII GTNAC 2 cut(s) 313, 1202
MalI GATC 4 cut(s) 6, 152, 626, 1152
MboI GATC 4 cut(s) 4, 150, 624, 1150
MfeI CAATTG 1 cut(s) 137
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 691, 762, 1084
MlsI TGGCCA 1 cut(s) 310
MluCI AATT 7 cut(s) 97, 137, 198, 203, 331, 520, 565
MluNI TGGCCA 1 cut(s) 310
MlyI GAGTC 3 cut(s) 18, 272, 908
MmeI TCCRAC 2 cut(s) 463, 1053
Mox20I TGGCCA 1 cut(s) 310
MscI TGGCCA 1 cut(s) 310
MseI TTAA 4 cut(s) 420, 861, 990, 1058
MslI CAYNNNNRTG 2 cut(s) 304, 755
Msp20I TGGCCA 1 cut(s) 310
MspA1I CMGCKG 2 cut(s) 361, 1103
MspI CCGG 7 cut(s) 257, 263, 391, 432, 742, 797, 1069
MspR9I CCNGG 5 cut(s) 257, 258, 392, 433, 798
MunI CAATTG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 257
MvnI CGCG 4 cut(s) 244, 374, 656, 1042
MwoI GCNNNNNNNGC 6 cut(s) 364, 373, 401, 695, 842, 1048
NciI CCSGG 5 cut(s) 257, 258, 392, 433, 798
NdeII GATC 4 cut(s) 4, 150, 624, 1150
NlaIV GGNNCC 7 cut(s) 6, 62, 70, 169, 261, 368, 448
NmeAIII GCCGAG 1 cut(s) 922
NmuCI GTSAC 1 cut(s) 313
PceI AGGCCT 1 cut(s) 404
PciSI GCTCTTC 1 cut(s) 348
PctI GAATGC 1 cut(s) 257
PfeI GAWTC 2 cut(s) 414, 1124
PflFI GACNNNGTC 1 cut(s) 35
PflMI CCANNNNNTGG 1 cut(s) 592
PinAI ACCGGT 1 cut(s) 1068
PkrI GCNGC 6 cut(s) 157, 215, 360, 410, 747, 1102
Ple19I CGATCG 1 cut(s) 153
PleI GAGTC 3 cut(s) 18, 272, 907
PpsI GAGTC 3 cut(s) 18, 272, 907
PpuMI RGGWCCY 1 cut(s) 68
Psp124BI GAGCTC 1 cut(s) 691
Psp5II RGGWCCY 1 cut(s) 68
PspFI CCCAGC 2 cut(s) 209, 729
PspN4I GGNNCC 7 cut(s) 6, 62, 70, 169, 261, 368, 448
PspPI GGNCC 6 cut(s) 68, 167, 260, 294, 393, 447
PspPPI RGGWCCY 1 cut(s) 68
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 35
PvuI CGATCG 1 cut(s) 153
PvuII CAGCTG 2 cut(s) 361, 1103
RsaI GTAC 2 cut(s) 146, 506
RsaNI GTAC 2 cut(s) 145, 505
RseI CAYNNNNRTG 2 cut(s) 304, 755
SacI GAGCTC 1 cut(s) 691
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 4 cut(s) 420, 861, 990, 1058
SatI GCNGC 6 cut(s) 156, 214, 359, 409, 746, 1101
Sau3AI GATC 4 cut(s) 4, 150, 624, 1150
Sau96I GGNCC 6 cut(s) 68, 167, 260, 294, 393, 447
SchI GAGTC 3 cut(s) 18, 272, 908
ScrFI CCNGG 5 cut(s) 257, 258, 392, 433, 798
SduI GDGCHC 3 cut(s) 691, 762, 1084
SfaNI GCATC 3 cut(s) 926, 955, 1102
SfiI GGCCNNNNNGGCC 1 cut(s) 401
SinI GGWCC 3 cut(s) 68, 260, 447
SmaI CCCGGG 1 cut(s) 258
SmiMI CAYNNNNRTG 2 cut(s) 304, 755
Sse9I AATT 7 cut(s) 97, 137, 198, 203, 331, 520, 565
SseBI AGGCCT 1 cut(s) 404
SsiI CCGC 8 cut(s) 126, 156, 242, 350, 408, 411, 656, 1042
SspMI CTAG 2 cut(s) 1128, 1207
SstI GAGCTC 1 cut(s) 691
StuI AGGCCT 1 cut(s) 404
StyD4I CCNGG 5 cut(s) 255, 256, 390, 431, 796
StyI CCWWGG 2 cut(s) 64, 396
TaaI ACNGT 1 cut(s) 1202
TaqI TCGA 1 cut(s) 662
TasI AATT 7 cut(s) 97, 137, 198, 203, 331, 520, 565
TatI WGTACW 1 cut(s) 504
TauI GCSGC 2 cut(s) 158, 411
TfiI GAWTC 2 cut(s) 414, 1124
Tru1I TTAA 4 cut(s) 420, 861, 990, 1058
Tru9I TTAA 4 cut(s) 420, 861, 990, 1058
TscAI CASTG 4 cut(s) 318, 1089, 1163, 1205
TseFI GTSAC 1 cut(s) 313
TseI GCWGC 4 cut(s) 213, 358, 745, 1100
Tsp45I GTSAC 1 cut(s) 313
TspDTI ATGAA 3 cut(s) 30, 819, 894
TspMI CCCGGG 1 cut(s) 256
TspRI CASTG 4 cut(s) 318, 1089, 1163, 1205
Tth111I GACNNNGTC 1 cut(s) 35
Van91I CCANNNNNTGG 1 cut(s) 592
VneI GTGCAC 1 cut(s) 1080
VpaK11BI GGWCC 3 cut(s) 68, 260, 447
XagI CCTNNNNNAGG 1 cut(s) 992
XapI RAATTY 2 cut(s) 331, 520
XcmI CCANNNNNNNNNTGG 1 cut(s) 331
XmaI CCCGGG 1 cut(s) 256
XspI CTAG 2 cut(s) 1128, 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.