Rorug05G0121200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000926
Physical Location & Seq
Reverse (-)
10697216 .. 10703389
6174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0121200.1

Sequence Viewer

Length: 615 bp
ATGACTTCTTCTGTTCACGATCATTCCGATAGTAGTGAATCTGATGATCAGCAAAAGCATTCAGAGTCTCCGATGATTTCTTCCTCACCTGCACCTGGAATGTCTAATCCTGGAATTCCAGGCCCTATGCAGTATGTAGCCCCTCCACAGATTGGAGCAGGACATGCAGTGGCGCCAGGAGCCTACCCATATCCGGACCCTTACTACAGAAGCATCTTTGCTCCTTATGACACACAACCATATCCTCCACAACCCTATGGGGGGCAACCAACGGTCCATCTTCAGTTAATGGGAATTCAGCAAGCAGGAGTTCCATTGCCAACAGACGCAGTTGAGGAACCTGTGTTTGTAAATGCAAAACAATATCATGGCATCTTGCGACGTCGGCAGTCTCGTGCTAAAGCAGAATCTGAAAACAAAGCTCTAAAATCTCGGAAGCCTTACTTGCATGAATCTCGACACCTGCATGCACTGAGAAGAGCTAGAGGATGTGGGGGTCGGTTTCTTAATGCAAAGAAAGATGAGAATGAACAGAGTGAAGGAGACAATTCACAGGCAATTATCAACTTGAACTCTGATAAAAGTGAGCGTTCTTCCTCGGATAGAACATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

204

Amino Acids

22.37

Weight (kDa)

7.14

Isoelectric Point (pI)

76.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFB_NFYA PF02045 113 - 168 7e-27 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 97, 471
AatII GACGTC 1 cut(s) 385
Acc36I ACCTGC 2 cut(s) 97, 471
AccB1I GGYRCC 1 cut(s) 172
AccB7I CCANNNNNTGG 1 cut(s) 152
AccIII TCCGGA 1 cut(s) 193
AcsI RAATTY 2 cut(s) 114, 294
AcuI CTGAAG 1 cut(s) 266
AcyI GRCGYC 2 cut(s) 173, 382
AfiI CCNNNNNNNGG 5 cut(s) 95, 152, 193, 260, 261
AgsI TTSAA 1 cut(s) 571
AjnI CCWGG 4 cut(s) 94, 109, 118, 175
AluBI AGCT 2 cut(s) 422, 482
AluI AGCT 2 cut(s) 422, 482
Alw26I GTCTC 3 cut(s) 72, 396, 537
AlwNI CAGNNNCTG 1 cut(s) 410
Aor13HI TCCGGA 1 cut(s) 193
AoxI GGCC 1 cut(s) 121
ApoI RAATTY 2 cut(s) 114, 294
AspLEI GCGC 1 cut(s) 175
AspS9I GGNCC 3 cut(s) 122, 196, 274
AsuHPI GGTGA 1 cut(s) 78
AvaII GGWCC 2 cut(s) 196, 274
BanI GGYRCC 1 cut(s) 172
BauI CACGAG 1 cut(s) 393
BccI CCATC 1 cut(s) 285
BcgI CGANNNNNNTGC 2 cut(s) 437, 471
BciT130I CCWGG 4 cut(s) 96, 111, 120, 177
BclI TGATCA 1 cut(s) 46
BcoDI GTCTC 3 cut(s) 72, 396, 537
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 1 cut(s) 205
BfoI RGCGCY 1 cut(s) 176
BfuAI ACCTGC 2 cut(s) 97, 471
Bme1390I CCNGG 4 cut(s) 96, 111, 120, 177
Bme18I GGWCC 2 cut(s) 196, 274
BmgT120I GGNCC 3 cut(s) 122, 196, 274
BmiI GGNNCC 4 cut(s) 174, 181, 198, 339
BmrFI CCNGG 4 cut(s) 96, 111, 120, 177
BmsI GCATC 2 cut(s) 222, 381
BsaHI GRCGYC 2 cut(s) 173, 382
BsaJI CCNNGG 1 cut(s) 597
BsaWI WCCGGW 1 cut(s) 193
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 152, 193, 260, 261
Bse3DI GCAATG 1 cut(s) 314
BseAI TCCGGA 1 cut(s) 193
BseBI CCWGG 4 cut(s) 96, 111, 120, 177
BseDI CCNNGG 1 cut(s) 597
BseGI GGATG 2 cut(s) 494, 608
BseLI CCNNNNNNNGG 5 cut(s) 95, 152, 193, 260, 261
BseMI GCAATG 1 cut(s) 314
BseMII CTCAG 1 cut(s) 464
BsgI GTGCAG 1 cut(s) 75
BshFI GGCC 1 cut(s) 123
BshNI GGYRCC 1 cut(s) 172
BsiSI CCGG 1 cut(s) 194
BslI CCNNNNNNNGG 5 cut(s) 95, 152, 193, 260, 261
BsmAI GTCTC 3 cut(s) 72, 396, 537
BsmI GAATGC 1 cut(s) 58
BsnI GGCC 1 cut(s) 123
Bsp13I TCCGGA 1 cut(s) 193
Bsp143I GATC 2 cut(s) 19, 46
BspANI GGCC 1 cut(s) 123
BspCNI CTCAG 1 cut(s) 465
BspEI TCCGGA 1 cut(s) 193
BspLI GGNNCC 4 cut(s) 174, 181, 198, 339
BspMI ACCTGC 2 cut(s) 97, 471
BspQI GCTCTTC 1 cut(s) 472
BspT107I GGYRCC 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 314
BssECI CCNNGG 1 cut(s) 597
BssMI GATC 2 cut(s) 19, 46
BssNI GRCGYC 2 cut(s) 173, 382
BssSI CACGAG 1 cut(s) 393
Bst2BI CACGAG 1 cut(s) 393
Bst2UI CCWGG 4 cut(s) 96, 111, 120, 177
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 1 cut(s) 472
BstACI GRCGYC 2 cut(s) 173, 382
BstAPI GCANNNNNTGC 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 303, 468
BstDEI CTNAG 1 cut(s) 473
BstF5I GGATG 2 cut(s) 494, 608
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 1 cut(s) 175
BstKTI GATC 2 cut(s) 22, 49
BstMAI GTCTC 3 cut(s) 72, 396, 537
BstMBI GATC 2 cut(s) 19, 46
BstMWI GCNNNNNNNGC 4 cut(s) 164, 179, 385, 445
BstNI CCWGG 4 cut(s) 96, 111, 120, 177
BstNSI RCATGY 2 cut(s) 167, 470
BstSCI CCNGG 4 cut(s) 94, 109, 118, 175
BstSFI CTRYAG 1 cut(s) 205
BsuRI GGCC 1 cut(s) 123
BtsCI GGATG 2 cut(s) 494, 608
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 2 cut(s) 174, 470
BveI ACCTGC 2 cut(s) 97, 471
Cac8I GCNNGC 2 cut(s) 303, 468
CaiI CAGNNNCTG 1 cut(s) 410
CfoI GCGC 1 cut(s) 175
Cfr13I GGNCC 3 cut(s) 122, 196, 274
CseI GACGC 1 cut(s) 335
CviAII CATG 4 cut(s) 164, 368, 449, 467
CviJI RGCY 6 cut(s) 123, 140, 182, 422, 439, 482
CviKI_1 RGCY 6 cut(s) 123, 140, 182, 422, 439, 482
DdeI CTNAG 1 cut(s) 473
DinI GGCGCC 1 cut(s) 174
DpnI GATC 2 cut(s) 21, 48
DpnII GATC 2 cut(s) 19, 46
Eam1104I CTCTTC 1 cut(s) 472
EarI CTCTTC 1 cut(s) 472
Eco47I GGWCC 2 cut(s) 196, 274
Eco57I CTGAAG 1 cut(s) 266
EcoO109I RGGNCCY 1 cut(s) 122
EcoRI GAATTC 2 cut(s) 114, 294
EcoRII CCWGG 4 cut(s) 94, 109, 118, 175
EgeI GGCGCC 1 cut(s) 174
EheI GGCGCC 1 cut(s) 174
FaeI CATG 4 cut(s) 167, 371, 452, 470
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FatI CATG 4 cut(s) 163, 367, 448, 466
FbaI TGATCA 1 cut(s) 46
FokI GGATG 2 cut(s) 501, 595
FspBI CTAG 1 cut(s) 483
GlaI GCGC 1 cut(s) 174
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 1 cut(s) 123
HapII CCGG 1 cut(s) 194
HgaI GACGC 1 cut(s) 335
HhaI GCGC 1 cut(s) 175
Hin1I GRCGYC 2 cut(s) 173, 382
Hin1II CATG 4 cut(s) 167, 371, 452, 470
Hin6I GCGC 1 cut(s) 173
HinP1I GCGC 1 cut(s) 173
HinfI GANTC 4 cut(s) 38, 65, 407, 452
HpaII CCGG 1 cut(s) 194
HphI GGTGA 1 cut(s) 78
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 8 cut(s) 28, 43, 64, 72, 412, 435, 577, 601
Hpy188III TCNNGA 3 cut(s) 17, 194, 456
Hpy8I GTNNAC 1 cut(s) 16
Hpy99I CGWCG 2 cut(s) 384, 387
HpyAV CCTTC 1 cut(s) 533
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 8 cut(s) 92, 130, 167, 356, 448, 466, 470, 512
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 179, 385, 445
HpyF3I CTNAG 1 cut(s) 473
HpySE526I ACGT 1 cut(s) 382
Hsp92I GRCGYC 2 cut(s) 173, 382
Hsp92II CATG 4 cut(s) 167, 371, 452, 470
HspAI GCGC 1 cut(s) 173
KasI GGCGCC 1 cut(s) 172
Kpn2I TCCGGA 1 cut(s) 193
Ksp22I TGATCA 1 cut(s) 46
Kzo9I GATC 2 cut(s) 19, 46
LguI GCTCTTC 1 cut(s) 472
LmnI GCTCC 3 cut(s) 155, 179, 226
LweI GCATC 2 cut(s) 222, 381
MaeI CTAG 1 cut(s) 483
MaeII ACGT 1 cut(s) 382
MalI GATC 2 cut(s) 21, 48
MboI GATC 2 cut(s) 19, 46
MboII GAAGA 4 cut(s) 72, 272, 489, 585
MluCI AATT 4 cut(s) 114, 294, 547, 558
Mly113I GGCGCC 1 cut(s) 173
MlyI GAGTC 1 cut(s) 74
MnlI CCTC 6 cut(s) 94, 153, 255, 328, 479, 607
MroI TCCGGA 1 cut(s) 193
MseI TTAA 2 cut(s) 287, 507
MslI CAYNNNNRTG 1 cut(s) 465
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 4 cut(s) 96, 111, 120, 177
Mva1269I GAATGC 1 cut(s) 58
MvaI CCWGG 4 cut(s) 96, 111, 120, 177
MwoI GCNNNNNNNGC 4 cut(s) 164, 179, 385, 445
NarI GGCGCC 1 cut(s) 173
NdeII GATC 2 cut(s) 19, 46
NlaIII CATG 4 cut(s) 167, 371, 452, 470
NlaIV GGNNCC 4 cut(s) 174, 181, 198, 339
NspI RCATGY 2 cut(s) 167, 470
PaeI GCATGC 1 cut(s) 470
PaqCI CACCTGC 2 cut(s) 97, 471
PciSI GCTCTTC 1 cut(s) 472
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 391
PctI GAATGC 1 cut(s) 58
PfeI GAWTC 3 cut(s) 38, 407, 452
PflMI CCANNNNNTGG 1 cut(s) 152
PfoI TCCNGGA 1 cut(s) 109
PleI GAGTC 1 cut(s) 73
PluTI GGCGCC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 73
Psp6I CCWGG 4 cut(s) 94, 109, 118, 175
PspGI CCWGG 4 cut(s) 94, 109, 118, 175
PspN4I GGNNCC 4 cut(s) 174, 181, 198, 339
PspPI GGNCC 3 cut(s) 122, 196, 274
PstNI CAGNNNCTG 1 cut(s) 410
RseI CAYNNNNRTG 1 cut(s) 465
SapI GCTCTTC 1 cut(s) 472
SaqAI TTAA 2 cut(s) 287, 507
Sau3AI GATC 2 cut(s) 19, 46
Sau96I GGNCC 3 cut(s) 122, 196, 274
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 4 cut(s) 96, 111, 120, 177
SetI ASST 7 cut(s) 91, 97, 343, 385, 424, 465, 484
SfaNI GCATC 2 cut(s) 222, 381
SfcI CTRYAG 1 cut(s) 205
SfoI GGCGCC 1 cut(s) 174
SinI GGWCC 2 cut(s) 196, 274
SmiMI CAYNNNNRTG 1 cut(s) 465
SphI GCATGC 1 cut(s) 470
Sse9I AATT 4 cut(s) 114, 294, 547, 558
SspDI GGCGCC 1 cut(s) 172
SspMI CTAG 1 cut(s) 483
StyD4I CCNGG 4 cut(s) 94, 109, 118, 175
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 1 cut(s) 385
TaqI TCGA 1 cut(s) 457
TasI AATT 4 cut(s) 114, 294, 547, 558
TfiI GAWTC 3 cut(s) 38, 407, 452
Tru1I TTAA 2 cut(s) 287, 507
Tru9I TTAA 2 cut(s) 287, 507
TscAI CASTG 2 cut(s) 174, 477
TspDTI ATGAA 2 cut(s) 465, 543
TspRI CASTG 2 cut(s) 174, 477
Van91I CCANNNNNTGG 1 cut(s) 152
VpaK11BI GGWCC 2 cut(s) 196, 274
XapI RAATTY 2 cut(s) 114, 294
XceI RCATGY 2 cut(s) 167, 470
XspI CTAG 1 cut(s) 483
ZraI GACGTC 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.