Rh4CG032500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000926
Physical Location & Seq
Forward (+)
6455223 .. 6456294
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG032500.1

Sequence Viewer

Length: 321 bp
ATGAGTGGCCATGATGTTTTCGTTTTAATGCCAATTGGAGGGGGTAAGAGCCTGACATATCAGAGTGAGCCTGAGTTAAATGTTGACCAGTTGGATAGTGCACTGAAAGACAATGTGGCAGCTGAAACTGATGCTCCTGCTGAATATAGTGAAAAAGAGGCATTTGATGATCATCAGTGCAATGGTAAAGAACCAATCATCTGTCAGAGCTTTTTGAAAGATGACTGTACAGATAAAAGCATAGAAAGTCTTGATGAGGGAAAGACAGTATTAATGGAGGAAGCAATTGGAGAAGAAGCAAAGGATGCATTGGTGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

11.57

Weight (kDa)

4.16

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 7
AfaI GTAC 1 cut(s) 229
AfiI CCNNNNNNNGG 1 cut(s) 38
AgsI TTSAA 1 cut(s) 217
AluBI AGCT 2 cut(s) 122, 210
AluI AGCT 2 cut(s) 122, 210
Alw21I GWGCWC 1 cut(s) 103
Alw44I GTGCAC 1 cut(s) 99
AoxI GGCC 1 cut(s) 7
ApaLI GTGCAC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 119
AseI ATTAAT 1 cut(s) 272
BaeGI GKGCMC 1 cut(s) 103
BalI TGGCCA 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 131
BclI TGATCA 1 cut(s) 169
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
BmsI GCATC 2 cut(s) 121, 295
BsaBI GATNNNNATC 1 cut(s) 171
BsaXI ACNNNNNCTCC 2 cut(s) 118, 148
Bsc4I CCNNNNNNNGG 1 cut(s) 38
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 187
Bse8I GATNNNNATC 1 cut(s) 171
BseGI GGATG 1 cut(s) 310
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 38
BseMI GCAATG 1 cut(s) 187
BseMII CTCAG 1 cut(s) 63
BseNI ACTGG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 103
BseXI GCAGC 1 cut(s) 131
BshFI GGCC 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 103
BslI CCNNNNNNNGG 1 cut(s) 38
BsnI GGCC 1 cut(s) 9
Bsp1286I GDGCHC 1 cut(s) 103
Bsp1407I TGTACA 1 cut(s) 227
Bsp143I GATC 1 cut(s) 169
BspANI GGCC 1 cut(s) 9
BspCNI CTCAG 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 187
BsrGI TGTACA 1 cut(s) 227
BsrI ACTGG 1 cut(s) 88
BssMI GATC 1 cut(s) 169
Bst4CI ACNGT 2 cut(s) 227, 268
BstAPI GCANNNNNTGC 1 cut(s) 305
BstAUI TGTACA 1 cut(s) 227
BstDEI CTNAG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 310
BstKTI GATC 1 cut(s) 172
BstMBI GATC 1 cut(s) 169
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstSLI GKGCMC 1 cut(s) 103
BstV1I GCAGC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 9
BtsCI GGATG 1 cut(s) 310
BtsIMutI CAGTG 2 cut(s) 101, 182
Csp6I GTAC 1 cut(s) 228
CviAII CATG 1 cut(s) 11
CviJI RGCY 5 cut(s) 9, 51, 70, 122, 210
CviKI_1 RGCY 5 cut(s) 9, 51, 70, 122, 210
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 1 cut(s) 72
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 7
EcoT22I ATGCAT 1 cut(s) 310
FaeI CATG 1 cut(s) 14
FaiI YATR 4 cut(s) 12, 58, 147, 242
FatI CATG 1 cut(s) 10
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 1 cut(s) 120
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 1 cut(s) 120
GluI GCNGC 1 cut(s) 120
HaeIII GGCC 1 cut(s) 9
Hin1II CATG 1 cut(s) 14
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
Hpy166II GTNNAC 2 cut(s) 85, 101
Hpy188I TCNGA 2 cut(s) 63, 207
Hpy188III TCNNGA 1 cut(s) 251
Hpy8I GTNNAC 2 cut(s) 85, 101
HpyCH4III ACNGT 2 cut(s) 227, 268
HpyCH4V TGCA 3 cut(s) 101, 180, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 1 cut(s) 72
Hsp92II CATG 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 1 cut(s) 169
LmnI GCTCC 1 cut(s) 139
LpnPI CCDG 4 cut(s) 65, 84, 101, 150
Lsp1109I GCAGC 1 cut(s) 131
LweI GCATC 2 cut(s) 121, 295
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 1 cut(s) 305
MfeI CAATTG 2 cut(s) 33, 285
MhlI GDGCHC 1 cut(s) 103
MlsI TGGCCA 1 cut(s) 9
MluCI AATT 2 cut(s) 33, 285
MluNI TGGCCA 1 cut(s) 9
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 4 cut(s) 32, 151, 250, 271
Mox20I TGGCCA 1 cut(s) 9
Mph1103I ATGCAT 1 cut(s) 310
MscI TGGCCA 1 cut(s) 9
MseI TTAA 3 cut(s) 26, 77, 272
Msp20I TGGCCA 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 122
MunI CAATTG 2 cut(s) 33, 285
MwoI GCNNNNNNNGC 1 cut(s) 305
NdeII GATC 1 cut(s) 169
NlaIII CATG 1 cut(s) 14
NsiI ATGCAT 1 cut(s) 310
PkrI GCNGC 1 cut(s) 121
PshBI ATTAAT 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
SaqAI TTAA 3 cut(s) 26, 77, 272
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 1 cut(s) 169
SduI GDGCHC 1 cut(s) 103
SetI ASST 2 cut(s) 124, 212
SfaNI GCATC 2 cut(s) 121, 295
SgeI CNNG 6 cut(s) 23, 64, 83, 100, 149, 263
Sse9I AATT 2 cut(s) 33, 285
TaaI ACNGT 2 cut(s) 227, 268
TasI AATT 2 cut(s) 33, 285
TatI WGTACW 1 cut(s) 227
Tru1I TTAA 3 cut(s) 26, 77, 272
Tru9I TTAA 3 cut(s) 26, 77, 272
TscAI CASTG 2 cut(s) 108, 182
TseI GCWGC 1 cut(s) 119
TspRI CASTG 2 cut(s) 108, 182
VneI GTGCAC 1 cut(s) 99
VspI ATTAAT 1 cut(s) 272
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.