MD11G1263900.v1.1

Polynucleotide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005030
Physical Location & Seq
Forward (+)
37893640 .. 37900157
6518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1263900.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGCTAACCATACCCACCCGCAACTCTATCTTCACCCACCTGATTCCCTTCCTCCCCAAAAACCCTAATTCCCCGAAACCCTCTCCGACACGTTTCGTCAATTTCCACACCCCGAAACGTTTCGCTTGTATCGGCCTCGACACACCACCAATGTCGGCTTCTTCTTCGACCAAATTGGCGGCGGAGTACGCGAAGTCGAAGAGGTCATCGTGCAAAAAGTGCTCGGAACCGATCGCCGCCCAGGCTCTGCGGCTGGGGTTGGTCAGCAGAGACCCTAAAGGTTTCGATGTGACCAAATGGCACCATTTGGATTGCTTCAATTTTAGGTCCGACTTGGTTGCTTCGGTTGAGAAGATTAAAGGGTTTCAGTCTTTGAAGAGCAGTGATCAGGAGGCTTTGAAGAAGTTAGTGGGTGCGTCACATGAATCTCGAGAGGAGGATACAAATGAAAGTAAATATGCAATTGAAGGAAAGCAAGGTTCTCATGGAAAAAGGGTTTGTGAAGTTGATGAAAATGAAAATGAAATAGAGGAAAGGAGTCCGAAGAAAGCAAAGTCCTCTAAATTTGATGGACAAGCTAAGCTGGAAATATCCCTTTCAATTTCTGATGTGAAAGACAAGTACAAGGATGCAACTCTTTCACCTAAATGGAAAGCATTTCAAACAATCATATTTCTTGAACGGGATGAAGGTCTACATGATTCGAGTAAAATTGCTGCTTTTGATTTTGATGGATGTCTTGCCAACACTAATGTAAAGAGAGTAGGTCCTGATGCTTGGTCTCTTATGTATCCTTCAATACCTGATAAGCTGCAGAGCCTGTACAAAGATGGCTACAAGCTGGTAATCTTTACGAATGAATCCAACATAGAGCGCTGGAAGAATAAGAGACAAGTAGCTGTGGACTCAAAAATTGGGCGCCTCAACAATTTTATTGGGCAAGTAAGGGTCCCAATTCAGGTTTTCATAGCTTGTGGCCTAGGCAAAGTTGGTAAAGCAGAAGACCCCTTTCGCAAGCCCAAACCTGGGATGTGGCATATTTTGGAACAACACTTCAACTCTGGCATTTCCATCGACATGGATCAATCCTTTTATGTTGGTGATGCAGCCGGGAGACAAAAGGATCATAGTGATGCTGATATTAAATTTGCGGAGGCTGTTGGTGTTAAGTTTTACGTCCCAGAGGAATACTTTGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

401

Amino Acids

44.85

Weight (kDa)

8.98

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-PARP PF00645 62 - 136 8.2e-17 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PNK3P PF08645 237 - 398 1.5e-47 Polynucleotide kinase 3 phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 300, 918
AccI GTMKAC 1 cut(s) 694
AccII CGCG 1 cut(s) 191
AciI CCGC 6 cut(s) 19, 179, 182, 237, 250, 1151
AclI AACGTT 1 cut(s) 118
AclWI GGATC 2 cut(s) 1089, 1131
AcsI RAATTY 2 cut(s) 563, 1145
AcyI GRCGYC 1 cut(s) 919
AfaI GTAC 3 cut(s) 188, 623, 824
AfeI AGCGCT 1 cut(s) 875
AfiI CCNNNNNNNGG 3 cut(s) 958, 1025, 1026
AflIII ACRYGT 1 cut(s) 89
AgsI TTSAA 9 cut(s) 319, 376, 400, 467, 600, 662, 680, 798, 1057
AjnI CCWGG 2 cut(s) 240, 1024
AjuI GAANNNNNNNTTGG 2 cut(s) 142, 174
AluBI AGCT 6 cut(s) 578, 583, 811, 841, 899, 971
AluI AGCT 6 cut(s) 578, 583, 811, 841, 899, 971
Alw21I GWGCWC 1 cut(s) 224
Alw26I GTCTC 4 cut(s) 264, 786, 883, 1108
AlwI GGATC 2 cut(s) 1089, 1131
AlwNI CAGNNNCTG 2 cut(s) 247, 820
Ama87I CYCGRG 1 cut(s) 429
Aor51HI AGCGCT 1 cut(s) 875
AoxI GGCC 2 cut(s) 133, 976
ApeKI GCWGC 3 cut(s) 716, 811, 1106
ApoI RAATTY 2 cut(s) 563, 1145
Asp700I GAANNNNTTC 1 cut(s) 119
AspA2I CCTAGG 1 cut(s) 979
AspLEI GCGC 2 cut(s) 876, 921
AspS9I GGNCC 3 cut(s) 327, 767, 949
AsuC2I CCSGG 1 cut(s) 1111
AsuHPI GGTGA 3 cut(s) 25, 633, 1112
AvaI CYCGRG 1 cut(s) 429
AvaII GGWCC 3 cut(s) 327, 767, 949
AvrII CCTAGG 1 cut(s) 979
BanI GGYRCC 2 cut(s) 300, 918
BbsI GAAGAC 1 cut(s) 1008
Bbv12I GWGCWC 1 cut(s) 224
BbvI GCAGC 3 cut(s) 703, 798, 1118
BccI CCATC 4 cut(s) 563, 725, 824, 1079
BcgI CGANNNNNNTGC 4 cut(s) 320, 354, 1054, 1088
BciT130I CCWGG 2 cut(s) 242, 1026
BciVI GTATCC 2 cut(s) 433, 801
BclI TGATCA 1 cut(s) 385
BcnI CCSGG 1 cut(s) 1111
BcoDI GTCTC 4 cut(s) 264, 786, 883, 1108
BfaI CTAG 1 cut(s) 980
BfmI CTRYAG 1 cut(s) 812
BfoI RGCGCY 2 cut(s) 877, 922
BfuI GTATCC 2 cut(s) 433, 801
BglI GCCNNNNNGGC 1 cut(s) 242
BisI GCNGC 6 cut(s) 180, 237, 251, 717, 812, 1107
BlnI CCTAGG 1 cut(s) 979
BlpI GCTNAGC 1 cut(s) 579
BlsI GCNGC 6 cut(s) 181, 238, 252, 718, 813, 1108
Bme1390I CCNGG 3 cut(s) 242, 1026, 1111
Bme18I GGWCC 3 cut(s) 327, 767, 949
BmeT110I CYCGRG 1 cut(s) 429
BmgT120I GGNCC 3 cut(s) 327, 767, 949
BmiI GGNNCC 5 cut(s) 228, 302, 920, 950, 951
BmrFI CCNGG 3 cut(s) 242, 1026, 1111
BmsI GCATC 4 cut(s) 619, 763, 1093, 1123
BpiI GAAGAC 1 cut(s) 1008
Bpu1102I GCTNAGC 1 cut(s) 579
BpuMI CCSGG 1 cut(s) 1111
BsaHI GRCGYC 1 cut(s) 919
BsaI GGTCTC 2 cut(s) 264, 786
BsaJI CCNNGG 3 cut(s) 240, 979, 1025
Bsc4I CCNNNNNNNGG 3 cut(s) 958, 1025, 1026
BseBI CCWGG 2 cut(s) 242, 1026
BseDI CCNNGG 3 cut(s) 240, 979, 1025
BseGI GGATG 4 cut(s) 634, 691, 740, 1035
BseLI CCNNNNNNNGG 3 cut(s) 958, 1025, 1026
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 3 cut(s) 703, 798, 1118
BseYI CCCAGC 1 cut(s) 253
Bsh1236I CGCG 1 cut(s) 191
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 2 cut(s) 135, 978
BshNI GGYRCC 2 cut(s) 300, 918
BsiEI CGRYCG 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 224
BsiHKCI CYCGRG 1 cut(s) 429
BsiSI CCGG 1 cut(s) 1110
BslFI GGGAC 2 cut(s) 935, 1163
BslI CCNNNNNNNGG 3 cut(s) 958, 1025, 1026
BsmAI GTCTC 4 cut(s) 264, 786, 883, 1108
BsmFI GGGAC 2 cut(s) 935, 1163
BsnI GGCC 2 cut(s) 135, 978
Bso31I GGTCTC 2 cut(s) 264, 786
BsoBI CYCGRG 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 822
Bsp143I GATC 4 cut(s) 231, 385, 1081, 1123
Bsp1720I GCTNAGC 1 cut(s) 579
BspACI CCGC 6 cut(s) 19, 179, 182, 237, 250, 1151
BspANI GGCC 2 cut(s) 135, 978
BspFNI CGCG 1 cut(s) 191
BspLI GGNNCC 5 cut(s) 228, 302, 920, 950, 951
BspMAI CTGCAG 1 cut(s) 816
BspPI GGATC 2 cut(s) 1089, 1131
BspQI GCTCTTC 1 cut(s) 371
BspT107I GGYRCC 2 cut(s) 300, 918
BspTNI GGTCTC 2 cut(s) 264, 786
BsrGI TGTACA 1 cut(s) 822
BssECI CCNNGG 3 cut(s) 240, 979, 1025
BssMI GATC 4 cut(s) 231, 385, 1081, 1123
BssNI GRCGYC 1 cut(s) 919
BssT1I CCWWGG 1 cut(s) 979
Bst2UI CCWGG 2 cut(s) 242, 1026
Bst6I CTCTTC 2 cut(s) 194, 371
BstACI GRCGYC 1 cut(s) 919
BstAPI GCANNNNNTGC 1 cut(s) 219
BstAUI TGTACA 1 cut(s) 822
BstC8I GCNNGC 1 cut(s) 1016
BstDEI CTNAG 1 cut(s) 579
BstF5I GGATG 4 cut(s) 634, 691, 740, 1035
BstFNI CGCG 1 cut(s) 191
BstH2I RGCGCY 2 cut(s) 877, 922
BstHHI GCGC 2 cut(s) 876, 921
BstKTI GATC 4 cut(s) 234, 388, 1084, 1126
BstMAI GTCTC 4 cut(s) 264, 786, 883, 1108
BstMBI GATC 4 cut(s) 231, 385, 1081, 1123
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 3 cut(s) 188, 219, 242
BstNI CCWGG 2 cut(s) 242, 1026
BstSCI CCNGG 3 cut(s) 240, 1024, 1109
BstSFI CTRYAG 1 cut(s) 812
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 3 cut(s) 703, 798, 1118
BstV2I GAAGAC 1 cut(s) 1008
BstXI CCANNNNNNTGG 1 cut(s) 1078
BsuI GTATCC 2 cut(s) 433, 801
BsuRI GGCC 2 cut(s) 135, 978
BtsCI GGATG 4 cut(s) 634, 691, 740, 1035
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 388
Cac8I GCNNGC 1 cut(s) 1016
CaiI CAGNNNCTG 2 cut(s) 247, 820
CfoI GCGC 2 cut(s) 876, 921
Cfr13I GGNCC 3 cut(s) 327, 767, 949
CseI GACGC 1 cut(s) 405
Csp6I GTAC 3 cut(s) 187, 622, 823
CviAII CATG 4 cut(s) 422, 485, 698, 1078
CviQI GTAC 3 cut(s) 187, 622, 823
DdeI CTNAG 1 cut(s) 579
DinI GGCGCC 1 cut(s) 920
DpnI GATC 4 cut(s) 233, 387, 1083, 1125
DpnII GATC 4 cut(s) 231, 385, 1081, 1123
Eam1104I CTCTTC 2 cut(s) 194, 371
EarI CTCTTC 2 cut(s) 194, 371
EciI GGCGGA 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 979
Eco31I GGTCTC 2 cut(s) 264, 786
Eco47I GGWCC 3 cut(s) 327, 767, 949
Eco47III AGCGCT 1 cut(s) 875
Eco88I CYCGRG 1 cut(s) 429
EcoO109I RGGNCCY 2 cut(s) 767, 949
EcoRII CCWGG 2 cut(s) 240, 1024
EcoT14I CCWWGG 1 cut(s) 979
EgeI GGCGCC 1 cut(s) 920
EheI GGCGCC 1 cut(s) 920
ErhI CCWWGG 1 cut(s) 979
FaeI CATG 4 cut(s) 425, 488, 701, 1081
FaqI GGGAC 2 cut(s) 935, 1163
FatI CATG 4 cut(s) 421, 484, 697, 1077
FauI CCCGC 1 cut(s) 26
FbaI TGATCA 1 cut(s) 385
FblI GTMKAC 1 cut(s) 694
Fnu4HI GCNGC 6 cut(s) 180, 237, 251, 717, 812, 1107
FokI GGATG 4 cut(s) 641, 698, 747, 1042
Fsp4HI GCNGC 6 cut(s) 180, 237, 251, 717, 812, 1107
FspBI CTAG 1 cut(s) 980
GlaI GCGC 2 cut(s) 875, 920
GluI GCNGC 6 cut(s) 180, 237, 251, 717, 812, 1107
GsaI CCCAGC 1 cut(s) 257
HaeII RGCGCY 2 cut(s) 877, 922
HaeIII GGCC 2 cut(s) 135, 978
HapII CCGG 1 cut(s) 1110
HgaI GACGC 1 cut(s) 405
HhaI GCGC 2 cut(s) 876, 921
Hin1I GRCGYC 1 cut(s) 919
Hin1II CATG 4 cut(s) 425, 488, 701, 1081
Hin6I GCGC 2 cut(s) 874, 919
HinP1I GCGC 2 cut(s) 874, 919
HinfI GANTC 6 cut(s) 43, 425, 538, 701, 860, 905
HpaII CCGG 1 cut(s) 1110
HphI GGTGA 3 cut(s) 25, 633, 1112
Hpy166II GTNNAC 2 cut(s) 695, 904
Hpy188I TCNGA 5 cut(s) 87, 226, 331, 543, 607
Hpy188III TCNNGA 5 cut(s) 389, 429, 431, 677, 770
Hpy8I GTNNAC 2 cut(s) 695, 904
HpyAV CCTTC 4 cut(s) 58, 461, 683, 804
HpyCH4IV ACGT 3 cut(s) 91, 118, 1176
HpyCH4V TGCA 5 cut(s) 213, 461, 632, 814, 1106
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 219, 242
HpyF3I CTNAG 1 cut(s) 579
HpySE526I ACGT 3 cut(s) 91, 118, 1176
Hsp92I GRCGYC 1 cut(s) 919
Hsp92II CATG 4 cut(s) 425, 488, 701, 1081
HspAI GCGC 2 cut(s) 874, 919
KasI GGCGCC 1 cut(s) 918
KflI GGGWCCC 1 cut(s) 949
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 4 cut(s) 231, 385, 1081, 1123
LguI GCTCTTC 1 cut(s) 371
Lsp1109I GCAGC 3 cut(s) 703, 798, 1118
LweI GCATC 4 cut(s) 619, 763, 1093, 1123
MaeI CTAG 1 cut(s) 980
MaeII ACGT 3 cut(s) 91, 118, 1176
MaeIII GTNAC 2 cut(s) 289, 417
MalI GATC 4 cut(s) 233, 387, 1083, 1125
MboI GATC 4 cut(s) 231, 385, 1081, 1123
MfeI CAATTG 1 cut(s) 462
MhlI GDGCHC 1 cut(s) 224
Mly113I GGCGCC 1 cut(s) 919
MlyI GAGTC 2 cut(s) 547, 899
MmeI TCCRAC 3 cut(s) 110, 354, 888
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 3 cut(s) 357, 1143, 1167
MslI CAYNNNNRTG 3 cut(s) 646, 1076, 1131
MspI CCGG 1 cut(s) 1110
MspR9I CCNGG 3 cut(s) 242, 1026, 1111
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 2 cut(s) 242, 1026
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 3 cut(s) 188, 219, 242
NarI GGCGCC 1 cut(s) 919
NciI CCSGG 1 cut(s) 1111
NdeII GATC 4 cut(s) 231, 385, 1081, 1123
NlaIII CATG 4 cut(s) 425, 488, 701, 1081
NlaIV GGNNCC 5 cut(s) 228, 302, 920, 950, 951
NmuCI GTSAC 2 cut(s) 289, 417
PaeR7I CTCGAG 1 cut(s) 429
PciSI GCTCTTC 1 cut(s) 371
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 4 cut(s) 43, 425, 701, 860
PkrI GCNGC 6 cut(s) 181, 238, 252, 718, 813, 1108
Ple19I CGATCG 1 cut(s) 234
PleI GAGTC 2 cut(s) 546, 899
PluTI GGCGCC 1 cut(s) 922
PpsI GAGTC 2 cut(s) 546, 899
PpuMI RGGWCCY 2 cut(s) 767, 949
Psp1406I AACGTT 1 cut(s) 118
Psp5II RGGWCCY 2 cut(s) 767, 949
Psp6I CCWGG 2 cut(s) 240, 1024
PspFI CCCAGC 1 cut(s) 253
PspGI CCWGG 2 cut(s) 240, 1024
PspN4I GGNNCC 5 cut(s) 228, 302, 920, 950, 951
PspPI GGNCC 3 cut(s) 327, 767, 949
PspPPI RGGWCCY 2 cut(s) 767, 949
PstI CTGCAG 1 cut(s) 816
PstNI CAGNNNCTG 2 cut(s) 247, 820
PvuI CGATCG 1 cut(s) 234
RsaI GTAC 3 cut(s) 188, 623, 824
RsaNI GTAC 3 cut(s) 187, 622, 823
RseI CAYNNNNRTG 3 cut(s) 646, 1076, 1131
SapI GCTCTTC 1 cut(s) 371
SaqAI TTAA 3 cut(s) 357, 1143, 1167
SatI GCNGC 6 cut(s) 180, 237, 251, 717, 812, 1107
Sau3AI GATC 4 cut(s) 231, 385, 1081, 1123
Sau96I GGNCC 3 cut(s) 327, 767, 949
SchI GAGTC 2 cut(s) 547, 899
ScrFI CCNGG 3 cut(s) 242, 1026, 1111
SduI GDGCHC 1 cut(s) 224
SfaNI GCATC 4 cut(s) 619, 763, 1093, 1123
SfcI CTRYAG 1 cut(s) 812
SfoI GGCGCC 1 cut(s) 920
Sfr274I CTCGAG 1 cut(s) 429
SinI GGWCC 3 cut(s) 327, 767, 949
SlaI CTCGAG 1 cut(s) 429
SmiMI CAYNNNNRTG 3 cut(s) 646, 1076, 1131
SmlI CTYRAG 1 cut(s) 429
SmoI CTYRAG 1 cut(s) 429
SsiI CCGC 6 cut(s) 19, 179, 182, 237, 250, 1151
SspDI GGCGCC 1 cut(s) 918
SspMI CTAG 1 cut(s) 980
StyD4I CCNGG 3 cut(s) 240, 1024, 1109
StyI CCWWGG 1 cut(s) 979
TaiI ACGT 3 cut(s) 94, 121, 1179
TaqI TCGA 7 cut(s) 138, 167, 197, 285, 430, 704, 1074
TatI WGTACW 2 cut(s) 621, 822
TauI GCSGC 3 cut(s) 182, 239, 253
TfiI GAWTC 4 cut(s) 43, 425, 701, 860
Tru1I TTAA 3 cut(s) 357, 1143, 1167
Tru9I TTAA 3 cut(s) 357, 1143, 1167
TscAI CASTG 1 cut(s) 388
TseFI GTSAC 2 cut(s) 289, 417
TseI GCWGC 3 cut(s) 716, 811, 1106
Tsp45I GTSAC 2 cut(s) 289, 417
TspDTI ATGAA 8 cut(s) 438, 462, 525, 531, 537, 702, 873, 955
TspRI CASTG 1 cut(s) 388
VpaK11BI GGWCC 3 cut(s) 327, 767, 949
XapI RAATTY 2 cut(s) 563, 1145
XhoI CTCGAG 1 cut(s) 429
XmaJI CCTAGG 1 cut(s) 979
XmiI GTMKAC 1 cut(s) 694
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 1 cut(s) 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.