Rroxscaffold_1G00049370

Polynucleotide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005030
Physical Location & Seq
Forward (+)
69543487 .. 69548339
4853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00049370.1

Sequence Viewer

Length: 1176 bp
ATGCTACCCATCTTTAGTTTCCACGCAACCGTCTGTAACTCGAGCATCTCTTGCCTAATCATCCCCGCCTTCCCAAAACCCCTAATTCCAAGTCATTCTCCGAAACGTTTCTTCCAAACCCCGAAACGTTTCGCCTGTATTCGCTCCCAAAAACCCCCGATGTCTTCTTCTTCTTCTTCGACCCATGTCACCGCCGAGTATGCCAAGTCCAACAGGTCCTCTTGCAAGAAGTGCTCGAAAAAGATCGAGGCCAAAACTCTGAGGCTGGGCTTGGTCACCAGAGACGCGCGTGGTTTTGACGCGATCAAGTGGCACCACCTGGGTTGCTTCAGTTTTGGGTCCGAGTTGGTTGCTTCGGTTGACAAGATTAAAGGGTTCCAGTCTCTAAAGAGCAGTGATCAGGAAGCTTTGAAGAATTTGGTGGATGAATTTGAGAAATCCAAAGAGGATTCAAATGGAAAGAAAGGTTCTCAAAAACAAAAGGTTTGTGATGTAGATGAAGATGAAAATGAAGTAGGGGAAAGAGAGTTGAAGAAAGCGAAGGTATCTAACCTGGAAATGTCCTTTTCAGTGTCTGATGTGAAGGACAAGTACAAGGAAGCAACTCTTCCACCTAAATGGAAGGCATTTCAGACTATCATATTTCTTGAAAGGGATGATGGTCTGCATGATTCAAGTAAAATTGCTGCTTTTGATTTTGATGGATGTCTTGCAAATACTGATGTTAGGAGAGTCGGTGCTGATGCTTGGTCCCTAATGTATCCTTCAATACCTGATAAACTACGGAGCTTGTATGAGGATGGCTACAAGCTGGTAATCTTCACAAATGAAGCCAACATTGAGCGCTGGAAAAATAAGAGACAAGTAGCTGTGGACTCAAAAATTGGACGTCTCAACGATTTTATTAAGGAAGTGAAGGTCCCAATTCAGGTTTTCATAGCTTGTGGGACGAGTCAAGGAGAAGACCCCTTCCGCAAACCCAAACCGGGGTTGTGGCATATGCTGGAACAACACTTCAACTCTGGCATTTCCATTGATATGAATCAGTTATTGGACTCATCTTTTTATGTTGGTGATGCTGCTGGAAGAAGCAATGATCACAGTGATGCTGATCTTAAGTTTGCAGAGGCCAATGGTTTAAAGTTTTATGTCCCAGAGGATTTCTTTGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

391

Amino Acids

43.97

Weight (kDa)

8.41

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-PARP PF00645 66 - 140 2e-17 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PNK3P PF08645 227 - 389 3.2e-44 Polynucleotide kinase 3 phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 892
AccB1I GGYRCC 1 cut(s) 312
AccII CGCG 3 cut(s) 287, 289, 302
AciI CCGC 3 cut(s) 66, 192, 973
AclI AACGTT 2 cut(s) 106, 127
AcsI RAATTY 2 cut(s) 415, 428
AcuI CTGAAG 1 cut(s) 313
AcyI GRCGYC 1 cut(s) 889
AfaI GTAC 1 cut(s) 593
AfeI AGCGCT 1 cut(s) 845
AfiI CCNNNNNNNGG 3 cut(s) 928, 986, 987
AflII CTTAAG 1 cut(s) 1115
AgsI TTSAA 8 cut(s) 412, 453, 532, 650, 675, 768, 1018, 1169
AjnI CCWGG 2 cut(s) 318, 552
AjuI GAANNNNNNNTTGG 2 cut(s) 1034, 1066
AluBI AGCT 6 cut(s) 407, 789, 811, 869, 941, 1172
AluI AGCT 6 cut(s) 407, 789, 811, 869, 941, 1172
Alw21I GWGCWC 1 cut(s) 236
Alw26I GTCTC 4 cut(s) 276, 387, 853, 896
AlwNI CAGNNNCTG 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 40
Aor51HI AGCGCT 1 cut(s) 845
AoxI GGCC 2 cut(s) 249, 1128
ApeKI GCWGC 2 cut(s) 686, 1079
ApoI RAATTY 2 cut(s) 415, 428
Asp700I GAANNNNTTC 2 cut(s) 107, 128
AspLEI GCGC 2 cut(s) 289, 846
AspS9I GGNCC 4 cut(s) 216, 339, 750, 919
AsuC2I CCSGG 1 cut(s) 987
AsuHPI GGTGA 3 cut(s) 181, 268, 1085
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 4 cut(s) 216, 339, 750, 919
BanI GGYRCC 1 cut(s) 312
BarI GAAGNNNNNNTAC 2 cut(s) 575, 607
BbsI GAAGAC 2 cut(s) 156, 969
Bbv12I GWGCWC 1 cut(s) 236
BbvI GCAGC 2 cut(s) 673, 1066
BccI CCATC 4 cut(s) 17, 653, 695, 794
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 2 cut(s) 320, 554
BciVI GTATCC 1 cut(s) 771
BclI TGATCA 2 cut(s) 397, 1096
BcnI CCSGG 1 cut(s) 987
BcoDI GTCTC 4 cut(s) 276, 387, 853, 896
BfoI RGCGCY 1 cut(s) 847
BfrI CTTAAG 1 cut(s) 1115
BfuI GTATCC 1 cut(s) 771
BisI GCNGC 2 cut(s) 687, 1080
BlsI GCNGC 2 cut(s) 688, 1081
Bme1390I CCNGG 3 cut(s) 320, 554, 987
Bme18I GGWCC 4 cut(s) 216, 339, 750, 919
BmeT110I CYCGRG 1 cut(s) 40
BmgT120I GGNCC 4 cut(s) 216, 339, 750, 919
BmiI GGNNCC 5 cut(s) 314, 340, 377, 752, 921
BmrFI CCNGG 3 cut(s) 320, 554, 987
BmsI GCATC 4 cut(s) 54, 733, 1066, 1096
BoxI GACNNNNGTC 1 cut(s) 185
BpiI GAAGAC 2 cut(s) 156, 969
BpuMI CCSGG 1 cut(s) 987
BsaBI GATNNNNATC 2 cut(s) 1041, 1110
BsaHI GRCGYC 1 cut(s) 889
BsaJI CCNNGG 2 cut(s) 319, 986
BsaXI ACNNNNNCTCC 2 cut(s) 721, 751
Bsc4I CCNNNNNNNGG 3 cut(s) 928, 986, 987
Bse1I ACTGG 1 cut(s) 379
Bse3DI GCAATG 1 cut(s) 1099
Bse8I GATNNNNATC 2 cut(s) 1041, 1110
BseBI CCWGG 2 cut(s) 320, 554
BseDI CCNNGG 2 cut(s) 319, 986
BseGI GGATG 5 cut(s) 60, 430, 661, 710, 805
BseJI GATNNNNATC 2 cut(s) 1041, 1110
BseLI CCNNNNNNNGG 3 cut(s) 928, 986, 987
BseMI GCAATG 1 cut(s) 1099
BseMII CTCAG 1 cut(s) 251
BseNI ACTGG 1 cut(s) 379
BseXI GCAGC 2 cut(s) 673, 1066
BseYI CCCAGC 1 cut(s) 265
Bsh1236I CGCG 3 cut(s) 287, 289, 302
BshFI GGCC 2 cut(s) 251, 1130
BshNI GGYRCC 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 236
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 1 cut(s) 986
BslFI GGGAC 4 cut(s) 736, 905, 961, 1136
BslI CCNNNNNNNGG 3 cut(s) 928, 986, 987
BsmAI GTCTC 4 cut(s) 276, 387, 853, 896
BsmBI CGTCTC 2 cut(s) 276, 896
BsmFI GGGAC 4 cut(s) 736, 905, 961, 1136
BsnI GGCC 2 cut(s) 251, 1130
BsoBI CYCGRG 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 236
Bsp143I GATC 5 cut(s) 243, 303, 397, 1096, 1111
BspACI CCGC 3 cut(s) 66, 192, 973
BspANI GGCC 2 cut(s) 251, 1130
BspCNI CTCAG 1 cut(s) 252
BspFNI CGCG 3 cut(s) 287, 289, 302
BspLI GGNNCC 5 cut(s) 314, 340, 377, 752, 921
BspT107I GGYRCC 1 cut(s) 312
BspTI CTTAAG 1 cut(s) 1115
BsrDI GCAATG 1 cut(s) 1099
BsrI ACTGG 1 cut(s) 379
BssECI CCNNGG 2 cut(s) 319, 986
BssMI GATC 5 cut(s) 243, 303, 397, 1096, 1111
BssNI GRCGYC 1 cut(s) 889
Bst2UI CCWGG 2 cut(s) 320, 554
Bst4CI ACNGT 2 cut(s) 31, 1103
Bst6I CTCTTC 1 cut(s) 612
BstACI GRCGYC 1 cut(s) 889
BstAFI CTTAAG 1 cut(s) 1115
BstAPI GCANNNNNTGC 2 cut(s) 51, 231
BstDEI CTNAG 1 cut(s) 260
BstEII GGTNACC 1 cut(s) 274
BstF5I GGATG 5 cut(s) 60, 430, 661, 710, 805
BstFNI CGCG 3 cut(s) 287, 289, 302
BstH2I RGCGCY 1 cut(s) 847
BstHHI GCGC 2 cut(s) 289, 846
BstKTI GATC 5 cut(s) 246, 306, 400, 1099, 1114
BstMAI GTCTC 4 cut(s) 276, 387, 853, 896
BstMBI GATC 5 cut(s) 243, 303, 397, 1096, 1111
BstMWI GCNNNNNNNGC 3 cut(s) 51, 200, 231
BstNI CCWGG 2 cut(s) 320, 554
BstPAI GACNNNNGTC 1 cut(s) 185
BstPI GGTNACC 1 cut(s) 274
BstSCI CCNGG 3 cut(s) 318, 552, 985
BstUI CGCG 3 cut(s) 287, 289, 302
BstV1I GCAGC 2 cut(s) 673, 1066
BstV2I GAAGAC 2 cut(s) 156, 969
BstXI CCANNNNNNTGG 1 cut(s) 618
BsuI GTATCC 1 cut(s) 771
BsuRI GGCC 2 cut(s) 251, 1130
BtsCI GGATG 5 cut(s) 60, 430, 661, 710, 805
BtsI GCAGTG 1 cut(s) 400
BtsIMutI CAGTG 3 cut(s) 400, 576, 1108
CaiI CAGNNNCTG 1 cut(s) 575
CfoI GCGC 2 cut(s) 289, 846
Cfr13I GGNCC 4 cut(s) 216, 339, 750, 919
CseI GACGC 2 cut(s) 293, 308
Csp6I GTAC 1 cut(s) 592
CspCI CAANNNNNGTGG 2 cut(s) 305, 340
CviAII CATG 2 cut(s) 185, 668
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 1 cut(s) 260
DpnI GATC 5 cut(s) 245, 305, 399, 1098, 1113
DpnII GATC 5 cut(s) 243, 303, 397, 1096, 1111
DraI TTTAAA 1 cut(s) 1140
Eam1104I CTCTTC 1 cut(s) 612
EarI CTCTTC 1 cut(s) 612
Eco47I GGWCC 4 cut(s) 216, 339, 750, 919
Eco47III AGCGCT 1 cut(s) 845
Eco57I CTGAAG 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 40
Eco91I GGTNACC 1 cut(s) 274
EcoO109I RGGNCCY 2 cut(s) 216, 919
EcoO65I GGTNACC 1 cut(s) 274
EcoRII CCWGG 2 cut(s) 318, 552
Esp3I CGTCTC 2 cut(s) 276, 896
FaeI CATG 2 cut(s) 188, 671
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FaqI GGGAC 4 cut(s) 736, 905, 961, 1136
FatI CATG 2 cut(s) 184, 667
FauI CCCGC 1 cut(s) 73
FauNDI CATATG 1 cut(s) 999
FbaI TGATCA 2 cut(s) 397, 1096
Fnu4HI GCNGC 2 cut(s) 687, 1080
FokI GGATG 5 cut(s) 47, 437, 668, 717, 812
Fsp4HI GCNGC 2 cut(s) 687, 1080
GlaI GCGC 2 cut(s) 288, 845
GluI GCNGC 2 cut(s) 687, 1080
GsaI CCCAGC 1 cut(s) 269
HaeII RGCGCY 1 cut(s) 847
HaeIII GGCC 2 cut(s) 251, 1130
HapII CCGG 1 cut(s) 986
HgaI GACGC 2 cut(s) 293, 308
HhaI GCGC 2 cut(s) 289, 846
Hin1I GRCGYC 1 cut(s) 889
Hin1II CATG 2 cut(s) 188, 671
Hin6I GCGC 2 cut(s) 287, 844
HinP1I GCGC 2 cut(s) 287, 844
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 2 cut(s) 405, 1170
HinfI GANTC 7 cut(s) 449, 671, 732, 875, 952, 1042, 1055
HpaII CCGG 1 cut(s) 986
HphI GGTGA 3 cut(s) 181, 268, 1085
Hpy166II GTNNAC 2 cut(s) 361, 874
Hpy188I TCNGA 5 cut(s) 102, 261, 343, 577, 633
Hpy188III TCNNGA 2 cut(s) 401, 647
Hpy8I GTNNAC 2 cut(s) 361, 874
HpyAV CCTTC 7 cut(s) 79, 535, 577, 616, 774, 910, 979
HpyCH4III ACNGT 2 cut(s) 31, 1103
HpyCH4IV ACGT 3 cut(s) 106, 127, 889
HpyCH4V TGCA 4 cut(s) 225, 667, 713, 1124
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 200, 231
HpyF3I CTNAG 1 cut(s) 260
HpySE526I ACGT 3 cut(s) 106, 127, 889
Hsp92I GRCGYC 1 cut(s) 889
Hsp92II CATG 2 cut(s) 188, 671
HspAI GCGC 2 cut(s) 287, 844
Ksp22I TGATCA 2 cut(s) 397, 1096
Kzo9I GATC 5 cut(s) 243, 303, 397, 1096, 1111
LmnI GCTCC 2 cut(s) 149, 786
Lsp1109I GCAGC 2 cut(s) 673, 1066
LweI GCATC 4 cut(s) 54, 733, 1066, 1096
MaeII ACGT 3 cut(s) 106, 127, 889
MaeIII GTNAC 3 cut(s) 35, 187, 274
MalI GATC 5 cut(s) 245, 305, 399, 1098, 1113
MboI GATC 5 cut(s) 243, 303, 397, 1096, 1111
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 6 cut(s) 84, 415, 428, 681, 882, 924
MlyI GAGTC 4 cut(s) 741, 869, 961, 1049
MmeI TCCRAC 1 cut(s) 234
MnlI CCTC 7 cut(s) 229, 241, 255, 439, 790, 1120, 1150
MroXI GAANNNNTTC 2 cut(s) 107, 128
MseI TTAA 4 cut(s) 369, 906, 1116, 1139
MslI CAYNNNNRTG 3 cut(s) 616, 1037, 1104
MspCI CTTAAG 1 cut(s) 1115
MspI CCGG 1 cut(s) 986
MspR9I CCNGG 3 cut(s) 320, 554, 987
MvaI CCWGG 2 cut(s) 320, 554
MvnI CGCG 3 cut(s) 287, 289, 302
MwoI GCNNNNNNNGC 3 cut(s) 51, 200, 231
NciI CCSGG 1 cut(s) 987
NdeI CATATG 1 cut(s) 999
NdeII GATC 5 cut(s) 243, 303, 397, 1096, 1111
NlaIII CATG 2 cut(s) 188, 671
NlaIV GGNNCC 5 cut(s) 314, 340, 377, 752, 921
NmeAIII GCCGAG 1 cut(s) 220
NmuCI GTSAC 2 cut(s) 187, 274
PaeR7I CTCGAG 1 cut(s) 40
PdmI GAANNNNTTC 2 cut(s) 107, 128
PfeI GAWTC 3 cut(s) 449, 671, 1042
PkrI GCNGC 2 cut(s) 688, 1081
PleI GAGTC 4 cut(s) 740, 869, 960, 1049
PpsI GAGTC 4 cut(s) 740, 869, 960, 1049
PpuMI RGGWCCY 2 cut(s) 216, 919
PshAI GACNNNNGTC 1 cut(s) 185
Psp1406I AACGTT 2 cut(s) 106, 127
Psp5II RGGWCCY 2 cut(s) 216, 919
Psp6I CCWGG 2 cut(s) 318, 552
PspEI GGTNACC 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 265
PspGI CCWGG 2 cut(s) 318, 552
PspN4I GGNNCC 5 cut(s) 314, 340, 377, 752, 921
PspPI GGNCC 4 cut(s) 216, 339, 750, 919
PspPPI RGGWCCY 2 cut(s) 216, 919
PspXI VCTCGAGB 1 cut(s) 40
PstNI CAGNNNCTG 1 cut(s) 575
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
RseI CAYNNNNRTG 3 cut(s) 616, 1037, 1104
SaqAI TTAA 4 cut(s) 369, 906, 1116, 1139
SatI GCNGC 2 cut(s) 687, 1080
Sau3AI GATC 5 cut(s) 243, 303, 397, 1096, 1111
Sau96I GGNCC 4 cut(s) 216, 339, 750, 919
SchI GAGTC 4 cut(s) 741, 869, 961, 1049
ScrFI CCNGG 3 cut(s) 320, 554, 987
SduI GDGCHC 1 cut(s) 236
SfaNI GCATC 4 cut(s) 54, 733, 1066, 1096
Sfr274I CTCGAG 1 cut(s) 40
SinI GGWCC 4 cut(s) 216, 339, 750, 919
SlaI CTCGAG 1 cut(s) 40
SmiMI CAYNNNNRTG 3 cut(s) 616, 1037, 1104
SmlI CTYRAG 2 cut(s) 40, 1115
SmoI CTYRAG 2 cut(s) 40, 1115
Sse9I AATT 6 cut(s) 84, 415, 428, 681, 882, 924
SsiI CCGC 3 cut(s) 66, 192, 973
StyD4I CCNGG 3 cut(s) 318, 552, 985
TaaI ACNGT 2 cut(s) 31, 1103
TaiI ACGT 3 cut(s) 109, 130, 892
TaqI TCGA 4 cut(s) 41, 179, 236, 246
TasI AATT 6 cut(s) 84, 415, 428, 681, 882, 924
TatI WGTACW 1 cut(s) 591
TfiI GAWTC 3 cut(s) 449, 671, 1042
Tru1I TTAA 4 cut(s) 369, 906, 1116, 1139
Tru9I TTAA 4 cut(s) 369, 906, 1116, 1139
TscAI CASTG 3 cut(s) 400, 576, 1108
TseFI GTSAC 2 cut(s) 187, 274
TseI GCWGC 2 cut(s) 686, 1079
Tsp45I GTSAC 2 cut(s) 187, 274
TspDTI ATGAA 7 cut(s) 441, 513, 519, 525, 843, 925, 1055
TspGWI ACGGA 1 cut(s) 799
TspRI CASTG 3 cut(s) 400, 576, 1108
Vha464I CTTAAG 1 cut(s) 1115
VpaK11BI GGWCC 4 cut(s) 216, 339, 750, 919
XapI RAATTY 2 cut(s) 415, 428
XhoI CTCGAG 1 cut(s) 40
XmnI GAANNNNTTC 2 cut(s) 107, 128
ZraI GACGTC 1 cut(s) 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.