Rh5CG237400

Polynucleotide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005030
Physical Location & Seq
Reverse (-)
24622853 .. 24627786
4934 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG237400.1

Sequence Viewer

Length: 1146 bp
ATGCTACCCATCGCAATTGTCTGTAAATCGATCATCTCTTGCCTAATCATCCCCGCCTTCCCAAAACCCCTAATTCCGAGTCATTCTCCGAAACGTTTCTTCCAAACCCCGAAACGTTTCGCCTGTATTCGCTCCCAAAACCCCCCAATGTCTTCTTCTTCTTCTTCGACCCATGTCACCGCCGAGTACGCCAAGTCCAACAGGTCCTCTTGCAAGAAGTGCTCGAAAAAGATCGAGGCCAAAGCTCTGAGGCTGGGCTTGGTCACCAGAGACGCGCGTGGGTTTGACGCGATCAAGTGGCACCACCTGGGTTGCTTCAGTTTTGGGTCCGAGTTGGTTGCTTCGGTTGACAAGATTAAAGGGTTCCAGTCTCTAAAGAGCAGTGATCAGGAAGCTTTGAAGAATTTGGTGGATGAATTTGAGAAATCCAAAGAGGAGGATTCAAATGGTTCTCAAAAACAAAAGGTTTGTGAAGTAGATGAAGATGAAAATGAAGTAGGGGAAAGGGATTTGAAGAAAGCGAAGGTATCTAACCTGGAAATATCCTTTTCAGTGTCTGATGTGAAGGACAAGTACAAGGAAGCAACTCTTCCACCTAAATGGAAGGCATTTCAGACTATCATATTTCTTGAAAGGGATGATGGTCTGCATGATTCAAGTAAAATTGCTGCTTTTGATTTTGATGGATGTCTTGCAAATACTGATGTTAGGAGAGTCGGTGCTGATGCTTGGTCCCTAATGTATCCTTCAATACCTGATAAACTACGGAGCTTGTATGAGGATGGCTACAAGCTGGTAATCTTCACGAATGAAGCCAACATTGAGCGCTGGAAAAATAAGAGACAAGTAGCTGTGGACTCAAAAATTGGACGTCTCAACGATTTTATTAAGGAAGTGAAGGTCCCAATTCAGGTTTTCATAGCTTGTGGGACGAGTCAAGGAGAAGACCCCTTTCGCAAACCCAAACCGGGGTTGTGGCACATGTTGGAACAACACTTCAACTCTGGCATTTCCATTGATATGAATCAATCTTTTTATGTTGGTGATGCTGCTGGAAGAAGCAATGATCACAGTGATGCTGATCTTAAGTTTGCAGAGGCCAATGGTTTAAAGTTTTATGTCCCAGAGGATTTCTTTGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

381

Amino Acids

42.83

Weight (kDa)

7.97

Isoelectric Point (pI)

46.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-PARP PF00645 62 - 136 2.3e-17 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PNK3P PF08645 221 - 379 2.4e-46 Polynucleotide kinase 3 phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 874
AccB1I GGYRCC 1 cut(s) 300
AccII CGCG 3 cut(s) 275, 277, 290
AciI CCGC 2 cut(s) 54, 180
AclI AACGTT 2 cut(s) 94, 115
AcsI RAATTY 2 cut(s) 403, 416
AcuI CTGAAG 1 cut(s) 301
AcyI GRCGYC 1 cut(s) 871
AfaI GTAC 2 cut(s) 188, 575
AfeI AGCGCT 1 cut(s) 827
AfiI CCNNNNNNNGG 3 cut(s) 910, 968, 969
AflII CTTAAG 1 cut(s) 1085
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 8 cut(s) 400, 444, 514, 632, 657, 750, 1000, 1139
AjnI CCWGG 2 cut(s) 306, 534
AjuI GAANNNNNNNTTGG 2 cut(s) 139, 171
AluBI AGCT 7 cut(s) 245, 395, 771, 793, 851, 923, 1142
AluI AGCT 7 cut(s) 245, 395, 771, 793, 851, 923, 1142
Alw21I GWGCWC 1 cut(s) 224
Alw26I GTCTC 4 cut(s) 264, 375, 835, 878
AlwNI CAGNNNCTG 1 cut(s) 557
Aor51HI AGCGCT 1 cut(s) 827
AoxI GGCC 2 cut(s) 237, 1098
ApeKI GCWGC 2 cut(s) 668, 1049
ApoI RAATTY 2 cut(s) 403, 416
Asp700I GAANNNNTTC 2 cut(s) 95, 116
AspLEI GCGC 2 cut(s) 277, 828
AspS9I GGNCC 4 cut(s) 204, 327, 732, 901
AsuC2I CCSGG 1 cut(s) 969
AsuHPI GGTGA 3 cut(s) 169, 256, 1055
AvaII GGWCC 4 cut(s) 204, 327, 732, 901
BanI GGYRCC 1 cut(s) 300
BarI GAAGNNNNNNTAC 2 cut(s) 557, 589
BbsI GAAGAC 2 cut(s) 144, 951
Bbv12I GWGCWC 1 cut(s) 224
BbvI GCAGC 2 cut(s) 655, 1036
BccI CCATC 4 cut(s) 17, 635, 677, 776
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BciT130I CCWGG 2 cut(s) 308, 536
BciVI GTATCC 1 cut(s) 753
BclI TGATCA 2 cut(s) 385, 1066
BcnI CCSGG 1 cut(s) 969
BcoDI GTCTC 4 cut(s) 264, 375, 835, 878
BfoI RGCGCY 1 cut(s) 829
BfrI CTTAAG 1 cut(s) 1085
BfuI GTATCC 1 cut(s) 753
BisI GCNGC 2 cut(s) 669, 1050
BlsI GCNGC 2 cut(s) 670, 1051
Bme1390I CCNGG 3 cut(s) 308, 536, 969
Bme18I GGWCC 4 cut(s) 204, 327, 732, 901
BmgT120I GGNCC 4 cut(s) 204, 327, 732, 901
BmiI GGNNCC 5 cut(s) 302, 328, 365, 734, 903
BmrFI CCNGG 3 cut(s) 308, 536, 969
BmsI GCATC 3 cut(s) 715, 1036, 1066
BoxI GACNNNNGTC 1 cut(s) 173
BpiI GAAGAC 2 cut(s) 144, 951
BplI GAGNNNNNCTC 2 cut(s) 70, 102
BpuMI CCSGG 1 cut(s) 969
Bsa29I ATCGAT 1 cut(s) 29
BsaBI GATNNNNATC 2 cut(s) 1023, 1080
BsaHI GRCGYC 1 cut(s) 871
BsaJI CCNNGG 2 cut(s) 307, 968
BsaXI ACNNNNNCTCC 2 cut(s) 703, 733
Bsc4I CCNNNNNNNGG 3 cut(s) 910, 968, 969
Bse1I ACTGG 1 cut(s) 367
Bse3DI GCAATG 1 cut(s) 1069
Bse8I GATNNNNATC 2 cut(s) 1023, 1080
BseBI CCWGG 2 cut(s) 308, 536
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 307, 968
BseGI GGATG 5 cut(s) 48, 418, 643, 692, 787
BseJI GATNNNNATC 2 cut(s) 1023, 1080
BseLI CCNNNNNNNGG 3 cut(s) 910, 968, 969
BseMI GCAATG 1 cut(s) 1069
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 367
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 2 cut(s) 655, 1036
BseYI CCCAGC 1 cut(s) 253
Bsh1236I CGCG 3 cut(s) 275, 277, 290
BshFI GGCC 2 cut(s) 239, 1100
BshNI GGYRCC 1 cut(s) 300
BshVI ATCGAT 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 224
BsiSI CCGG 1 cut(s) 968
BslFI GGGAC 4 cut(s) 718, 887, 943, 1106
BslI CCNNNNNNNGG 3 cut(s) 910, 968, 969
BsmAI GTCTC 4 cut(s) 264, 375, 835, 878
BsmBI CGTCTC 2 cut(s) 264, 878
BsmFI GGGAC 4 cut(s) 718, 887, 943, 1106
BsnI GGCC 2 cut(s) 239, 1100
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 6 cut(s) 30, 231, 291, 385, 1066, 1081
BspACI CCGC 2 cut(s) 54, 180
BspANI GGCC 2 cut(s) 239, 1100
BspCNI CTCAG 1 cut(s) 240
BspDI ATCGAT 1 cut(s) 29
BspFNI CGCG 3 cut(s) 275, 277, 290
BspLI GGNNCC 5 cut(s) 302, 328, 365, 734, 903
BspT107I GGYRCC 1 cut(s) 300
BspTI CTTAAG 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 1069
BsrI ACTGG 1 cut(s) 367
BssECI CCNNGG 2 cut(s) 307, 968
BssMI GATC 6 cut(s) 30, 231, 291, 385, 1066, 1081
BssNI GRCGYC 1 cut(s) 871
Bst2UI CCWGG 2 cut(s) 308, 536
Bst4CI ACNGT 1 cut(s) 1073
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 871
BstAFI CTTAAG 1 cut(s) 1085
BstAPI GCANNNNNTGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 248
BstEII GGTNACC 1 cut(s) 262
BstF5I GGATG 5 cut(s) 48, 418, 643, 692, 787
BstFNI CGCG 3 cut(s) 275, 277, 290
BstH2I RGCGCY 1 cut(s) 829
BstHHI GCGC 2 cut(s) 277, 828
BstKTI GATC 6 cut(s) 33, 234, 294, 388, 1069, 1084
BstMAI GTCTC 4 cut(s) 264, 375, 835, 878
BstMBI GATC 6 cut(s) 30, 231, 291, 385, 1066, 1081
BstMWI GCNNNNNNNGC 2 cut(s) 188, 219
BstNI CCWGG 2 cut(s) 308, 536
BstNSI RCATGY 1 cut(s) 985
BstPAI GACNNNNGTC 1 cut(s) 173
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 3 cut(s) 306, 534, 967
BstUI CGCG 3 cut(s) 275, 277, 290
BstV1I GCAGC 2 cut(s) 655, 1036
BstV2I GAAGAC 2 cut(s) 144, 951
BstXI CCANNNNNNTGG 1 cut(s) 600
Bsu15I ATCGAT 1 cut(s) 29
BsuI GTATCC 1 cut(s) 753
BsuRI GGCC 2 cut(s) 239, 1100
BsuTUI ATCGAT 1 cut(s) 29
BtsCI GGATG 5 cut(s) 48, 418, 643, 692, 787
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 3 cut(s) 388, 558, 1078
CaiI CAGNNNCTG 1 cut(s) 557
CfoI GCGC 2 cut(s) 277, 828
Cfr13I GGNCC 4 cut(s) 204, 327, 732, 901
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 2 cut(s) 281, 296
Csp6I GTAC 2 cut(s) 187, 574
CspCI CAANNNNNGTGG 2 cut(s) 293, 328
CviAII CATG 3 cut(s) 173, 650, 982
CviQI GTAC 2 cut(s) 187, 574
DdeI CTNAG 1 cut(s) 248
DpnI GATC 6 cut(s) 32, 233, 293, 387, 1068, 1083
DpnII GATC 6 cut(s) 30, 231, 291, 385, 1066, 1081
DraI TTTAAA 1 cut(s) 1110
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco47I GGWCC 4 cut(s) 204, 327, 732, 901
Eco47III AGCGCT 1 cut(s) 827
Eco57I CTGAAG 1 cut(s) 301
Eco91I GGTNACC 1 cut(s) 262
EcoO109I RGGNCCY 2 cut(s) 204, 901
EcoO65I GGTNACC 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 306, 534
Esp3I CGTCTC 2 cut(s) 264, 878
FaeI CATG 3 cut(s) 176, 653, 985
FaiI YATR 9 cut(s) 174, 623, 651, 777, 920, 983, 1022, 1038, 1119
FalI AAGNNNNNCTT 2 cut(s) 573, 605
FaqI GGGAC 4 cut(s) 718, 887, 943, 1106
FatI CATG 3 cut(s) 172, 649, 981
FauI CCCGC 1 cut(s) 61
FbaI TGATCA 2 cut(s) 385, 1066
Fnu4HI GCNGC 2 cut(s) 669, 1050
FokI GGATG 5 cut(s) 35, 425, 650, 699, 794
Fsp4HI GCNGC 2 cut(s) 669, 1050
GlaI GCGC 2 cut(s) 276, 827
GluI GCNGC 2 cut(s) 669, 1050
GsaI CCCAGC 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 829
HaeIII GGCC 2 cut(s) 239, 1100
HapII CCGG 1 cut(s) 968
HgaI GACGC 2 cut(s) 281, 296
HhaI GCGC 2 cut(s) 277, 828
Hin1I GRCGYC 1 cut(s) 871
Hin1II CATG 3 cut(s) 176, 653, 985
Hin6I GCGC 2 cut(s) 275, 826
HinP1I GCGC 2 cut(s) 275, 826
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 2 cut(s) 393, 1140
HinfI GANTC 7 cut(s) 79, 440, 653, 714, 857, 934, 1024
HpaII CCGG 1 cut(s) 968
HphI GGTGA 3 cut(s) 169, 256, 1055
Hpy166II GTNNAC 2 cut(s) 349, 856
Hpy188I TCNGA 6 cut(s) 78, 90, 249, 331, 559, 615
Hpy188III TCNNGA 3 cut(s) 389, 629, 805
Hpy8I GTNNAC 2 cut(s) 349, 856
HpyAV CCTTC 6 cut(s) 67, 517, 559, 598, 756, 892
HpyCH4III ACNGT 1 cut(s) 1073
HpyCH4IV ACGT 3 cut(s) 94, 115, 871
HpyCH4V TGCA 4 cut(s) 213, 649, 695, 1094
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 219
HpyF3I CTNAG 1 cut(s) 248
HpySE526I ACGT 3 cut(s) 94, 115, 871
Hsp92I GRCGYC 1 cut(s) 871
Hsp92II CATG 3 cut(s) 176, 653, 985
HspAI GCGC 2 cut(s) 275, 826
Ksp22I TGATCA 2 cut(s) 385, 1066
Kzo9I GATC 6 cut(s) 30, 231, 291, 385, 1066, 1081
LmnI GCTCC 2 cut(s) 137, 768
Lsp1109I GCAGC 2 cut(s) 655, 1036
LweI GCATC 3 cut(s) 715, 1036, 1066
MaeII ACGT 3 cut(s) 94, 115, 871
MaeIII GTNAC 2 cut(s) 175, 262
MalI GATC 6 cut(s) 32, 233, 293, 387, 1068, 1083
MboI GATC 6 cut(s) 30, 231, 291, 385, 1066, 1081
MfeI CAATTG 1 cut(s) 15
MhlI GDGCHC 1 cut(s) 224
MluCI AATT 7 cut(s) 15, 72, 403, 416, 663, 864, 906
MlyI GAGTC 4 cut(s) 88, 723, 851, 943
MmeI TCCRAC 2 cut(s) 222, 966
MnlI CCTC 8 cut(s) 217, 229, 243, 427, 430, 772, 1090, 1120
MroXI GAANNNNTTC 2 cut(s) 95, 116
MseI TTAA 4 cut(s) 357, 888, 1086, 1109
MslI CAYNNNNRTG 3 cut(s) 598, 1019, 1074
MspCI CTTAAG 1 cut(s) 1085
MspI CCGG 1 cut(s) 968
MspR9I CCNGG 3 cut(s) 308, 536, 969
MunI CAATTG 1 cut(s) 15
MvaI CCWGG 2 cut(s) 308, 536
MvnI CGCG 3 cut(s) 275, 277, 290
MwoI GCNNNNNNNGC 2 cut(s) 188, 219
NciI CCSGG 1 cut(s) 969
NdeII GATC 6 cut(s) 30, 231, 291, 385, 1066, 1081
NlaIII CATG 3 cut(s) 176, 653, 985
NlaIV GGNNCC 5 cut(s) 302, 328, 365, 734, 903
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 2 cut(s) 175, 262
NspI RCATGY 1 cut(s) 985
PciI ACATGT 1 cut(s) 981
PdmI GAANNNNTTC 2 cut(s) 95, 116
PfeI GAWTC 3 cut(s) 440, 653, 1024
PkrI GCNGC 2 cut(s) 670, 1051
PleI GAGTC 4 cut(s) 87, 722, 851, 942
PpsI GAGTC 4 cut(s) 87, 722, 851, 942
PpuMI RGGWCCY 2 cut(s) 204, 901
PscI ACATGT 1 cut(s) 981
PshAI GACNNNNGTC 1 cut(s) 173
Psp1406I AACGTT 2 cut(s) 94, 115
Psp5II RGGWCCY 2 cut(s) 204, 901
Psp6I CCWGG 2 cut(s) 306, 534
PspEI GGTNACC 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 253
PspGI CCWGG 2 cut(s) 306, 534
PspN4I GGNNCC 5 cut(s) 302, 328, 365, 734, 903
PspPI GGNCC 4 cut(s) 204, 327, 732, 901
PspPPI RGGWCCY 2 cut(s) 204, 901
PstNI CAGNNNCTG 1 cut(s) 557
RsaI GTAC 2 cut(s) 188, 575
RsaNI GTAC 2 cut(s) 187, 574
RseI CAYNNNNRTG 3 cut(s) 598, 1019, 1074
SaqAI TTAA 4 cut(s) 357, 888, 1086, 1109
SatI GCNGC 2 cut(s) 669, 1050
Sau3AI GATC 6 cut(s) 30, 231, 291, 385, 1066, 1081
Sau96I GGNCC 4 cut(s) 204, 327, 732, 901
SchI GAGTC 4 cut(s) 88, 723, 851, 943
ScrFI CCNGG 3 cut(s) 308, 536, 969
SduI GDGCHC 1 cut(s) 224
SfaNI GCATC 3 cut(s) 715, 1036, 1066
SinI GGWCC 4 cut(s) 204, 327, 732, 901
SmiMI CAYNNNNRTG 3 cut(s) 598, 1019, 1074
SmlI CTYRAG 1 cut(s) 1085
SmoI CTYRAG 1 cut(s) 1085
Sse9I AATT 7 cut(s) 15, 72, 403, 416, 663, 864, 906
SsiI CCGC 2 cut(s) 54, 180
StyD4I CCNGG 3 cut(s) 306, 534, 967
TaaI ACNGT 1 cut(s) 1073
TaiI ACGT 3 cut(s) 97, 118, 874
TaqI TCGA 4 cut(s) 29, 167, 224, 234
TasI AATT 7 cut(s) 15, 72, 403, 416, 663, 864, 906
TatI WGTACW 1 cut(s) 573
TfiI GAWTC 3 cut(s) 440, 653, 1024
Tru1I TTAA 4 cut(s) 357, 888, 1086, 1109
Tru9I TTAA 4 cut(s) 357, 888, 1086, 1109
TscAI CASTG 3 cut(s) 388, 558, 1078
TseFI GTSAC 2 cut(s) 175, 262
TseI GCWGC 2 cut(s) 668, 1049
Tsp45I GTSAC 2 cut(s) 175, 262
TspDTI ATGAA 7 cut(s) 429, 495, 501, 507, 825, 907, 1037
TspGWI ACGGA 1 cut(s) 781
TspRI CASTG 3 cut(s) 388, 558, 1078
Vha464I CTTAAG 1 cut(s) 1085
VpaK11BI GGWCC 4 cut(s) 204, 327, 732, 901
XapI RAATTY 2 cut(s) 403, 416
XceI RCATGY 1 cut(s) 985
XmnI GAANNNNTTC 2 cut(s) 95, 116
ZraI GACGTC 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.