Rh5AG214500

Polynucleotide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005030
Physical Location & Seq
Reverse (-)
25249119 .. 25254198
5080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG214500.1

Sequence Viewer

Length: 1107 bp
ATGCTACCCATCGCAATTGTCTGTAAATCGATCATCTCTTGCCTAATCATTCCCGCCTTCCCAAAACCCCTAATTCCTTGTCATTCTCCGAAACGTTTCTTCCAAACCCCGAAACGTTTCGCCTGTATTCGCTCCCAAAAACCCCCAATGTCTTCTTCTTCTTCTTCTTTGACCCATGTCGCCGCCGAGTACGCCAAGTCCAACAGGTCCTCTTGCAAGAAGTGCTCGGAAAAGATCGAGGCCAAAGCTCTGAGGCTGGGCTTGGTCACCAGAGACGCGCGTGGGTTTGACGCGATCAAGTGGCACCACCTGGGTTGCTTCAGTTTTGGGTCCGAGTTGGTTGCTTCGGTTGACAAGATTAAAGGGTTTCAGTCTCTGAAGAGCAGTGATCAGGAAGCTTTGAAGAATTTGGTGGATGAATTTGAGAAATCCAAAGAGGAGGATTCAAATGGAAAGAAAGGTTCTCAAAAACAAAAGGTTTGTGAAGTAGATGAAGATGAAAATGAAGTAGGGGAAAGGGATTTGAAGAAAGCGAAGGTATCTAACCTGGAAATATCCTTTTCAGTGTCTGATGTGAAGGACAAGTACAAGGAAGCAACTCTTCCACCTAAATGGAAGGCATTTCAGACTATCATATTTCTTGAAAGGGATGATGGTCTGCATGATTCAAGTAAAATTGCTGCTTTTGATTTTGATGGATGTCTTGCAAATACTGATGTTAGGAGAGTCGGTGCTGATGCTTGGTCCCTAATGTATCCTTCTATACCTGATAAACTACGGAGCTTGTATGAGGATGGCTACAAGCTGGTAATCTTCACGAATGAAGCCAACATTGAGCGCTGGAAAAATAAGAGACAAGTAGCTGTGGACTCAAAAATTGGACGTCTCAACGATTTTATTAAGGAAGTGAAGGTCCCAATTCAGGTTTTCATAGCTTGTGGCACAAGCCAAGGAGAAGACCCCTTTCGCAAACCCAAACCGGGGTTGTGGCATATGTTGGAACAACACTTCAACTCTGGCATTTCCATTGATATGAATCAGTTATTGAACTCCTTTTTTTTTTTCTTTCTTTTTTCCTGGGATTACACATCACTACTTTTCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

368

Amino Acids

41.71

Weight (kDa)

8.75

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-PARP PF00645 63 - 137 2.5e-17 Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region
PNK3P PF08645 225 - 348 3.9e-27 Polynucleotide kinase 3 phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 886
AccB1I GGYRCC 1 cut(s) 303
AccII CGCG 3 cut(s) 278, 280, 293
AciI CCGC 2 cut(s) 54, 183
AclI AACGTT 2 cut(s) 94, 115
AcsI RAATTY 2 cut(s) 406, 419
AcuI CTGAAG 2 cut(s) 304, 398
AcyI GRCGYC 1 cut(s) 883
AfaI GTAC 2 cut(s) 191, 587
AfeI AGCGCT 1 cut(s) 839
AfiI CCNNNNNNNGG 3 cut(s) 922, 980, 981
AgsI TTSAA 7 cut(s) 403, 447, 526, 644, 669, 1012, 1048
AjnI CCWGG 3 cut(s) 309, 546, 1076
AjuI GAANNNNNNNTTGG 2 cut(s) 139, 171
AluBI AGCT 6 cut(s) 248, 398, 783, 805, 863, 935
AluI AGCT 6 cut(s) 248, 398, 783, 805, 863, 935
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 4 cut(s) 267, 378, 847, 890
AlwNI CAGNNNCTG 2 cut(s) 376, 569
Aor51HI AGCGCT 1 cut(s) 839
AoxI GGCC 1 cut(s) 240
ApeKI GCWGC 1 cut(s) 680
ApoI RAATTY 2 cut(s) 406, 419
Asp700I GAANNNNTTC 2 cut(s) 95, 116
AspLEI GCGC 2 cut(s) 280, 840
AspS9I GGNCC 4 cut(s) 207, 330, 744, 913
AsuC2I CCSGG 1 cut(s) 981
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 4 cut(s) 207, 330, 744, 913
BanI GGYRCC 1 cut(s) 303
BarI GAAGNNNNNNTAC 2 cut(s) 569, 601
BbsI GAAGAC 2 cut(s) 144, 963
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 1 cut(s) 667
BccI CCATC 4 cut(s) 17, 647, 689, 788
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 3 cut(s) 311, 548, 1078
BciVI GTATCC 1 cut(s) 765
BclI TGATCA 1 cut(s) 388
BcnI CCSGG 1 cut(s) 981
BcoDI GTCTC 4 cut(s) 267, 378, 847, 890
BfoI RGCGCY 1 cut(s) 841
BfuI GTATCC 1 cut(s) 765
BisI GCNGC 2 cut(s) 183, 681
BlsI GCNGC 2 cut(s) 184, 682
Bme1390I CCNGG 4 cut(s) 311, 548, 981, 1078
Bme18I GGWCC 4 cut(s) 207, 330, 744, 913
BmgT120I GGNCC 4 cut(s) 207, 330, 744, 913
BmiI GGNNCC 4 cut(s) 305, 331, 746, 915
BmrFI CCNGG 4 cut(s) 311, 548, 981, 1078
BmsI GCATC 1 cut(s) 727
BoxI GACNNNNGTC 1 cut(s) 176
BpiI GAAGAC 2 cut(s) 144, 963
BpuMI CCSGG 1 cut(s) 981
Bsa29I ATCGAT 1 cut(s) 29
BsaBI GATNNNNATC 1 cut(s) 1035
BsaHI GRCGYC 1 cut(s) 883
BsaJI CCNNGG 4 cut(s) 310, 949, 980, 1077
BsaXI ACNNNNNCTCC 2 cut(s) 715, 745
Bsc4I CCNNNNNNNGG 3 cut(s) 922, 980, 981
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 3 cut(s) 311, 548, 1078
BseCI ATCGAT 1 cut(s) 29
BseDI CCNNGG 4 cut(s) 310, 949, 980, 1077
BseGI GGATG 4 cut(s) 421, 655, 704, 799
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 3 cut(s) 922, 980, 981
BseMII CTCAG 1 cut(s) 242
BseRI GAGGAG 1 cut(s) 452
BseXI GCAGC 1 cut(s) 667
BseYI CCCAGC 1 cut(s) 256
Bsh1236I CGCG 3 cut(s) 278, 280, 293
BshFI GGCC 1 cut(s) 242
BshNI GGYRCC 1 cut(s) 303
BshVI ATCGAT 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 227
BsiSI CCGG 1 cut(s) 980
BslFI GGGAC 2 cut(s) 730, 899
BslI CCNNNNNNNGG 3 cut(s) 922, 980, 981
BsmAI GTCTC 4 cut(s) 267, 378, 847, 890
BsmBI CGTCTC 2 cut(s) 267, 890
BsmFI GGGAC 2 cut(s) 730, 899
BsnI GGCC 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 4 cut(s) 30, 234, 294, 388
BspACI CCGC 2 cut(s) 54, 183
BspANI GGCC 1 cut(s) 242
BspCNI CTCAG 1 cut(s) 243
BspDI ATCGAT 1 cut(s) 29
BspFNI CGCG 3 cut(s) 278, 280, 293
BspLI GGNNCC 4 cut(s) 305, 331, 746, 915
BspQI GCTCTTC 1 cut(s) 374
BspT107I GGYRCC 1 cut(s) 303
BssECI CCNNGG 4 cut(s) 310, 949, 980, 1077
BssMI GATC 4 cut(s) 30, 234, 294, 388
BssNI GRCGYC 1 cut(s) 883
BssT1I CCWWGG 1 cut(s) 949
Bst2UI CCWGG 3 cut(s) 311, 548, 1078
Bst6I CTCTTC 2 cut(s) 374, 606
BstACI GRCGYC 1 cut(s) 883
BstAPI GCANNNNNTGC 1 cut(s) 222
BstDEI CTNAG 1 cut(s) 251
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 4 cut(s) 421, 655, 704, 799
BstFNI CGCG 3 cut(s) 278, 280, 293
BstH2I RGCGCY 1 cut(s) 841
BstHHI GCGC 2 cut(s) 280, 840
BstKTI GATC 4 cut(s) 33, 237, 297, 391
BstMAI GTCTC 4 cut(s) 267, 378, 847, 890
BstMBI GATC 4 cut(s) 30, 234, 294, 388
BstMWI GCNNNNNNNGC 2 cut(s) 191, 222
BstNI CCWGG 3 cut(s) 311, 548, 1078
BstPAI GACNNNNGTC 1 cut(s) 176
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 4 cut(s) 309, 546, 979, 1076
BstUI CGCG 3 cut(s) 278, 280, 293
BstV1I GCAGC 1 cut(s) 667
BstV2I GAAGAC 2 cut(s) 144, 963
BstXI CCANNNNNNTGG 1 cut(s) 612
Bsu15I ATCGAT 1 cut(s) 29
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 1 cut(s) 242
BsuTUI ATCGAT 1 cut(s) 29
BtsCI GGATG 4 cut(s) 421, 655, 704, 799
BtsI GCAGTG 1 cut(s) 391
BtsIMutI CAGTG 2 cut(s) 391, 570
CaiI CAGNNNCTG 2 cut(s) 376, 569
CfoI GCGC 2 cut(s) 280, 840
Cfr13I GGNCC 4 cut(s) 207, 330, 744, 913
ClaI ATCGAT 1 cut(s) 29
CseI GACGC 2 cut(s) 284, 299
Csp6I GTAC 2 cut(s) 190, 586
CspCI CAANNNNNGTGG 2 cut(s) 296, 331
CviAII CATG 2 cut(s) 176, 662
CviQI GTAC 2 cut(s) 190, 586
DdeI CTNAG 1 cut(s) 251
DpnI GATC 4 cut(s) 32, 236, 296, 390
DpnII GATC 4 cut(s) 30, 234, 294, 388
Eam1104I CTCTTC 2 cut(s) 374, 606
EarI CTCTTC 2 cut(s) 374, 606
Eco130I CCWWGG 1 cut(s) 949
Eco47I GGWCC 4 cut(s) 207, 330, 744, 913
Eco47III AGCGCT 1 cut(s) 839
Eco57I CTGAAG 2 cut(s) 304, 398
Eco91I GGTNACC 1 cut(s) 265
EcoO109I RGGNCCY 2 cut(s) 207, 913
EcoO65I GGTNACC 1 cut(s) 265
EcoRII CCWGG 3 cut(s) 309, 546, 1076
EcoT14I CCWWGG 1 cut(s) 949
ErhI CCWWGG 1 cut(s) 949
Esp3I CGTCTC 2 cut(s) 267, 890
FaeI CATG 2 cut(s) 179, 665
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 2 cut(s) 730, 899
FatI CATG 2 cut(s) 175, 661
FauI CCCGC 1 cut(s) 61
FauNDI CATATG 1 cut(s) 993
FbaI TGATCA 1 cut(s) 388
Fnu4HI GCNGC 2 cut(s) 183, 681
FokI GGATG 4 cut(s) 428, 662, 711, 806
Fsp4HI GCNGC 2 cut(s) 183, 681
GlaI GCGC 2 cut(s) 279, 839
GluI GCNGC 2 cut(s) 183, 681
GsaI CCCAGC 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 841
HaeIII GGCC 1 cut(s) 242
HapII CCGG 1 cut(s) 980
HgaI GACGC 2 cut(s) 284, 299
HhaI GCGC 2 cut(s) 280, 840
Hin1I GRCGYC 1 cut(s) 883
Hin1II CATG 2 cut(s) 179, 665
Hin6I GCGC 2 cut(s) 278, 838
HinP1I GCGC 2 cut(s) 278, 838
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 5 cut(s) 443, 665, 726, 869, 1036
HpaII CCGG 1 cut(s) 980
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 352, 868
Hpy188I TCNGA 7 cut(s) 90, 229, 252, 334, 378, 571, 627
Hpy188III TCNNGA 3 cut(s) 392, 641, 817
Hpy8I GTNNAC 2 cut(s) 352, 868
HpyAV CCTTC 6 cut(s) 67, 529, 571, 610, 768, 904
HpyCH4IV ACGT 3 cut(s) 94, 115, 883
HpyCH4V TGCA 3 cut(s) 216, 661, 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 222
HpyF3I CTNAG 1 cut(s) 251
HpySE526I ACGT 3 cut(s) 94, 115, 883
Hsp92I GRCGYC 1 cut(s) 883
Hsp92II CATG 2 cut(s) 179, 665
HspAI GCGC 2 cut(s) 278, 838
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 4 cut(s) 30, 234, 294, 388
LguI GCTCTTC 1 cut(s) 374
LmnI GCTCC 2 cut(s) 137, 780
Lsp1109I GCAGC 1 cut(s) 667
LweI GCATC 1 cut(s) 727
MaeII ACGT 3 cut(s) 94, 115, 883
MaeIII GTNAC 1 cut(s) 265
MalI GATC 4 cut(s) 32, 236, 296, 390
MboI GATC 4 cut(s) 30, 234, 294, 388
MfeI CAATTG 1 cut(s) 15
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 7 cut(s) 15, 72, 406, 419, 675, 876, 918
MlyI GAGTC 2 cut(s) 735, 863
MmeI TCCRAC 2 cut(s) 225, 978
MnlI CCTC 6 cut(s) 220, 232, 246, 430, 433, 784
MroXI GAANNNNTTC 2 cut(s) 95, 116
MseI TTAA 2 cut(s) 360, 900
MslI CAYNNNNRTG 2 cut(s) 610, 1031
MspI CCGG 1 cut(s) 980
MspR9I CCNGG 4 cut(s) 311, 548, 981, 1078
MunI CAATTG 1 cut(s) 15
MvaI CCWGG 3 cut(s) 311, 548, 1078
MvnI CGCG 3 cut(s) 278, 280, 293
MwoI GCNNNNNNNGC 2 cut(s) 191, 222
NciI CCSGG 1 cut(s) 981
NdeI CATATG 1 cut(s) 993
NdeII GATC 4 cut(s) 30, 234, 294, 388
NlaIII CATG 2 cut(s) 179, 665
NlaIV GGNNCC 4 cut(s) 305, 331, 746, 915
NmeAIII GCCGAG 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 265
PciSI GCTCTTC 1 cut(s) 374
PdmI GAANNNNTTC 2 cut(s) 95, 116
PfeI GAWTC 3 cut(s) 443, 665, 1036
PkrI GCNGC 2 cut(s) 184, 682
PleI GAGTC 2 cut(s) 734, 863
PpsI GAGTC 2 cut(s) 734, 863
PpuMI RGGWCCY 2 cut(s) 207, 913
PshAI GACNNNNGTC 1 cut(s) 176
Psp1406I AACGTT 2 cut(s) 94, 115
Psp5II RGGWCCY 2 cut(s) 207, 913
Psp6I CCWGG 3 cut(s) 309, 546, 1076
PspEI GGTNACC 1 cut(s) 265
PspFI CCCAGC 1 cut(s) 256
PspGI CCWGG 3 cut(s) 309, 546, 1076
PspN4I GGNNCC 4 cut(s) 305, 331, 746, 915
PspPI GGNCC 4 cut(s) 207, 330, 744, 913
PspPPI RGGWCCY 2 cut(s) 207, 913
PstNI CAGNNNCTG 2 cut(s) 376, 569
RsaI GTAC 2 cut(s) 191, 587
RsaNI GTAC 2 cut(s) 190, 586
RseI CAYNNNNRTG 2 cut(s) 610, 1031
SapI GCTCTTC 1 cut(s) 374
SaqAI TTAA 2 cut(s) 360, 900
SatI GCNGC 2 cut(s) 183, 681
Sau3AI GATC 4 cut(s) 30, 234, 294, 388
Sau96I GGNCC 4 cut(s) 207, 330, 744, 913
SchI GAGTC 2 cut(s) 735, 863
ScrFI CCNGG 4 cut(s) 311, 548, 981, 1078
SduI GDGCHC 1 cut(s) 227
SfaNI GCATC 1 cut(s) 727
SinI GGWCC 4 cut(s) 207, 330, 744, 913
SmiMI CAYNNNNRTG 2 cut(s) 610, 1031
Sse9I AATT 7 cut(s) 15, 72, 406, 419, 675, 876, 918
SsiI CCGC 2 cut(s) 54, 183
StyD4I CCNGG 4 cut(s) 309, 546, 979, 1076
StyI CCWWGG 1 cut(s) 949
TaiI ACGT 3 cut(s) 97, 118, 886
TaqI TCGA 2 cut(s) 29, 237
TasI AATT 7 cut(s) 15, 72, 406, 419, 675, 876, 918
TatI WGTACW 1 cut(s) 585
TauI GCSGC 1 cut(s) 185
TfiI GAWTC 3 cut(s) 443, 665, 1036
Tru1I TTAA 2 cut(s) 360, 900
Tru9I TTAA 2 cut(s) 360, 900
TscAI CASTG 2 cut(s) 391, 570
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 1 cut(s) 680
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 7 cut(s) 432, 507, 513, 519, 837, 919, 1049
TspGWI ACGGA 1 cut(s) 793
TspRI CASTG 2 cut(s) 391, 570
VpaK11BI GGWCC 4 cut(s) 207, 330, 744, 913
XapI RAATTY 2 cut(s) 406, 419
XmnI GAANNNNTTC 2 cut(s) 95, 116
ZraI GACGTC 1 cut(s) 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.