Rh2DG254200

Polynucleotide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005030
Physical Location & Seq
Forward (+)
26678116 .. 26690598
12483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG254200.1

Sequence Viewer

Length: 663 bp
ATGACTTGCGTATCTAACCTGGAAATATCCTTTTCAGTGTCTGATGTGAAGGACAAGTACAAGGTTTTCATAGCTTGTGGGACGAGTCAAGGAGAAGACCCCTTTCGCAAACCCAAACCGGGGTTGTGGCATATGTTGGAACAACACTTCAACTCTGGCATTTCCATTGATATGAAACATTCCGCCGCTGCAACAGTCCAGTTGTTGGGTTGTTCACTGCCATTGCCAGCAACTAGAATCTCAGCTTGTTTCAATTCTACTACTCCAGCCGAATCCACAACTAGAATCTCAAATGCGCCACCGCTTCTCCGACCCCGTCAAGTAGTGGTCGGAGGCCGCCGGAAAGCTCTCCGGCCGGCGGCACCAACCTCCTCGAGAAGGGAAGCTTCGAGTACTACGCCGAACTCGCCTACTCGACCGCAAGCTCGTCGAGTCCACCGACAACCTCGCTTTCTCCGACGGCAACTCCCATTTTCAGATCACCTCTTTGGGATATTCGCATTATACGACGGTGCAATTGGGGACGCCAGGAATAAAAATTTCGAGGTCAGCAAATATGACCCCAAAGGGTCATCGACTAGCAAGAAAGCCCATTGCAGCGTGCAAAGTGGTTCATTCCTGGATATTGCTGCTTCTAATGGACCAAGAATGTTATCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0008150 GO:0008152 GO:0009117 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010638 GO:0010835 GO:0010836 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0019205 GO:0019219 GO:0019222 GO:0019637 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031400 GO:0031974 GO:0031981 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032268 GO:0032269 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0042578 GO:0042769 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045935 GO:0046403 GO:0046404 GO:0046483 GO:0046939 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051248 GO:0051338 GO:0051347 GO:0051606 GO:0051716 GO:0051731 GO:0051733 GO:0051734 GO:0051972 GO:0051973 GO:0055086 GO:0060255 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0098501 GO:0098502 GO:0098503 GO:0098504 GO:0098506 GO:0098518 GO:1901360 GO:1901363 GO:1904353 GO:1904355 GO:1904356 GO:1904358 GO:2000112 GO:2000278 GO:2000573 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNK3P zf-PARP

Protein Analysis

220

Amino Acids

24.06

Weight (kDa)

10.29

Isoelectric Point (pI)

53.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 361
AccB7I CCANNNNNTGG 1 cut(s) 205
AciI CCGC 6 cut(s) 183, 186, 302, 337, 359, 419
AcoI YGGCCR 1 cut(s) 353
AcsI RAATTY 1 cut(s) 538
AcyI GRCGYC 1 cut(s) 525
AfaI GTAC 2 cut(s) 59, 394
AfiI CCNNNNNNNGG 5 cut(s) 119, 120, 205, 358, 378
AgsI TTSAA 2 cut(s) 151, 253
AjnI CCWGG 3 cut(s) 18, 527, 618
AluBI AGCT 5 cut(s) 74, 245, 347, 386, 425
AluI AGCT 5 cut(s) 74, 245, 347, 386, 425
AlwNI CAGNNNCTG 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 373
AoxI GGCC 2 cut(s) 334, 353
ApeKI GCWGC 3 cut(s) 188, 597, 629
ApoI RAATTY 1 cut(s) 538
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 1 cut(s) 641
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 1 cut(s) 473
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 1 cut(s) 641
BaeI ACNNNNGTAYC 1 cut(s) 27
BanI GGYRCC 1 cut(s) 361
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BbsI GAAGAC 1 cut(s) 102
BbvI GCAGC 3 cut(s) 175, 609, 616
BceAI ACGGC 1 cut(s) 476
BcgI CGANNNNNNTGC 2 cut(s) 410, 444
BciT130I CCWGG 3 cut(s) 20, 529, 620
BcnI CCSGG 1 cut(s) 120
BfaI CTAG 4 cut(s) 234, 282, 579, 661
BisI GCNGC 6 cut(s) 186, 189, 337, 360, 598, 630
BlsI GCNGC 6 cut(s) 187, 190, 338, 361, 599, 631
BmcAI AGTACT 1 cut(s) 394
Bme1390I CCNGG 4 cut(s) 20, 120, 529, 620
Bme18I GGWCC 1 cut(s) 641
BmeT110I CYCGRG 1 cut(s) 373
BmgT120I GGNCC 1 cut(s) 641
BmiI GGNNCC 1 cut(s) 363
BmrFI CCNGG 4 cut(s) 20, 120, 529, 620
BpiI GAAGAC 1 cut(s) 102
BpmI CTGGAG 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 120
BsaHI GRCGYC 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 119
BsaXI ACNNNNNCTCC 4 cut(s) 291, 321, 450, 480
Bsc4I CCNNNNNNNGG 5 cut(s) 119, 120, 205, 358, 378
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 1 cut(s) 199
Bse3DI GCAATG 2 cut(s) 221, 592
BseBI CCWGG 3 cut(s) 20, 529, 620
BseDI CCNNGG 1 cut(s) 119
BseLI CCNNNNNNNGG 5 cut(s) 119, 120, 205, 358, 378
BseMI GCAATG 2 cut(s) 221, 592
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 361
BseX3I CGGCCG 1 cut(s) 353
BseXI GCAGC 3 cut(s) 175, 609, 616
Bsh1285I CGRYCG 2 cut(s) 356, 419
BshFI GGCC 2 cut(s) 336, 355
BshNI GGYRCC 1 cut(s) 361
BsiEI CGRYCG 2 cut(s) 356, 419
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 4 cut(s) 119, 340, 352, 356
BslFI GGGAC 2 cut(s) 94, 536
BslI CCNNNNNNNGG 5 cut(s) 119, 120, 205, 358, 378
BsmFI GGGAC 2 cut(s) 94, 536
BsnI GGCC 2 cut(s) 336, 355
BsoBI CYCGRG 1 cut(s) 373
Bsp143I GATC 1 cut(s) 478
BspACI CCGC 6 cut(s) 183, 186, 302, 337, 359, 419
BspANI GGCC 2 cut(s) 336, 355
BspCNI CTCAG 1 cut(s) 254
BspLI GGNNCC 1 cut(s) 363
BspT107I GGYRCC 1 cut(s) 361
BsrDI GCAATG 2 cut(s) 221, 592
BsrFI RCCGGY 1 cut(s) 355
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 1 cut(s) 478
BssNI GRCGYC 1 cut(s) 525
Bst2UI CCWGG 3 cut(s) 20, 529, 620
Bst4CI ACNGT 2 cut(s) 196, 512
BstACI GRCGYC 1 cut(s) 525
BstC8I GCNNGC 4 cut(s) 228, 357, 423, 602
BstDEI CTNAG 1 cut(s) 241
BstENI CCTNNNNNAGG 1 cut(s) 376
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 1 cut(s) 481
BstMBI GATC 1 cut(s) 478
BstMCI CGRYCG 2 cut(s) 356, 419
BstMWI GCNNNNNNNGC 1 cut(s) 406
BstNI CCWGG 3 cut(s) 20, 529, 620
BstSCI CCNGG 4 cut(s) 18, 118, 527, 618
BstV1I GCAGC 3 cut(s) 175, 609, 616
BstV2I GAAGAC 1 cut(s) 102
BstZI CGGCCG 1 cut(s) 353
BsuRI GGCC 2 cut(s) 336, 355
BtsI GCAGTG 1 cut(s) 215
BtsIMutI CAGTG 2 cut(s) 42, 215
Cac8I GCNNGC 4 cut(s) 228, 357, 423, 602
CaiI CAGNNNCTG 1 cut(s) 41
CfoI GCGC 1 cut(s) 298
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 1 cut(s) 641
CseI GACGC 1 cut(s) 533
Csp6I GTAC 2 cut(s) 58, 393
CviJI RGCY 9 cut(s) 74, 245, 269, 336, 347, 355, 386, 425, 590
CviKI_1 RGCY 9 cut(s) 74, 245, 269, 336, 347, 355, 386, 425, 590
CviQI GTAC 2 cut(s) 58, 393
DdeI CTNAG 1 cut(s) 241
DpnI GATC 1 cut(s) 480
DpnII GATC 1 cut(s) 478
EaeI YGGCCR 1 cut(s) 353
EagI CGGCCG 1 cut(s) 353
EciI GGCGGA 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 353
Eco47I GGWCC 1 cut(s) 641
Eco52I CGGCCG 1 cut(s) 353
Eco88I CYCGRG 1 cut(s) 373
EcoNI CCTNNNNNAGG 1 cut(s) 376
EcoRII CCWGG 3 cut(s) 18, 527, 618
FaiI YATR 6 cut(s) 71, 132, 134, 173, 505, 558
FalI AAGNNNNNCTT 2 cut(s) 370, 402
FaqI GGGAC 2 cut(s) 94, 536
FauNDI CATATG 1 cut(s) 132
Fnu4HI GCNGC 6 cut(s) 186, 189, 337, 360, 598, 630
Fsp4HI GCNGC 6 cut(s) 186, 189, 337, 360, 598, 630
FspBI CTAG 4 cut(s) 234, 282, 579, 661
GlaI GCGC 1 cut(s) 297
GluI GCNGC 6 cut(s) 186, 189, 337, 360, 598, 630
GsuI CTGGAG 1 cut(s) 249
HaeIII GGCC 2 cut(s) 336, 355
HapII CCGG 4 cut(s) 119, 340, 352, 356
HgaI GACGC 1 cut(s) 533
HhaI GCGC 1 cut(s) 298
Hin1I GRCGYC 1 cut(s) 525
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HindIII AAGCTT 1 cut(s) 384
HinfI GANTC 5 cut(s) 85, 237, 272, 285, 432
HpaII CCGG 4 cut(s) 119, 340, 352, 356
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 2 cut(s) 215, 436
Hpy188I TCNGA 5 cut(s) 43, 311, 332, 458, 478
Hpy188III TCNNGA 1 cut(s) 375
Hpy8I GTNNAC 2 cut(s) 215, 436
Hpy99I CGWCG 3 cut(s) 432, 462, 512
HpyAV CCTTC 2 cut(s) 43, 372
HpyCH4III ACNGT 2 cut(s) 196, 512
HpyCH4V TGCA 4 cut(s) 191, 515, 597, 604
HpyF10VI GCNNNNNNNGC 1 cut(s) 406
HpyF3I CTNAG 1 cut(s) 241
Hsp92I GRCGYC 1 cut(s) 525
HspAI GCGC 1 cut(s) 296
KroI GCCGGC 1 cut(s) 355
KroNI GCCGGC 1 cut(s) 357
Kzo9I GATC 1 cut(s) 478
Lsp1109I GCAGC 3 cut(s) 175, 609, 616
MaeI CTAG 4 cut(s) 234, 282, 579, 661
MalI GATC 1 cut(s) 480
MboI GATC 1 cut(s) 478
MboII GAAGA 1 cut(s) 107
MfeI CAATTG 1 cut(s) 516
MluCI AATT 3 cut(s) 253, 516, 538
MlyI GAGTC 2 cut(s) 94, 441
MmeI TCCRAC 4 cut(s) 117, 310, 334, 481
MnlI CCTC 6 cut(s) 326, 379, 382, 456, 494, 538
MroNI GCCGGC 1 cut(s) 355
MslI CAYNNNNRTG 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 4 cut(s) 119, 340, 352, 356
MspR9I CCNGG 4 cut(s) 20, 120, 529, 620
MunI CAATTG 1 cut(s) 516
MvaI CCWGG 3 cut(s) 20, 529, 620
MwoI GCNNNNNNNGC 1 cut(s) 406
NaeI GCCGGC 1 cut(s) 357
NciI CCSGG 1 cut(s) 120
NdeI CATATG 1 cut(s) 132
NdeII GATC 1 cut(s) 478
NgoMIV GCCGGC 1 cut(s) 355
NlaIV GGNNCC 1 cut(s) 363
PaeR7I CTCGAG 1 cut(s) 373
PcsI WCGNNNNNNNCGW 3 cut(s) 436, 454, 504
PdiI GCCGGC 1 cut(s) 357
PfeI GAWTC 3 cut(s) 237, 272, 285
PflFI GACNNNGTC 1 cut(s) 315
PflMI CCANNNNNTGG 1 cut(s) 205
PfoI TCCNGGA 1 cut(s) 618
PkrI GCNGC 6 cut(s) 187, 190, 338, 361, 599, 631
PleI GAGTC 2 cut(s) 93, 440
PpsI GAGTC 2 cut(s) 93, 440
Psp6I CCWGG 3 cut(s) 18, 527, 618
PspGI CCWGG 3 cut(s) 18, 527, 618
PspN4I GGNNCC 1 cut(s) 363
PspPI GGNCC 1 cut(s) 641
PstNI CAGNNNCTG 1 cut(s) 41
PsyI GACNNNGTC 1 cut(s) 315
RsaI GTAC 2 cut(s) 59, 394
RsaNI GTAC 2 cut(s) 58, 393
RseI CAYNNNNRTG 1 cut(s) 170
SatI GCNGC 6 cut(s) 186, 189, 337, 360, 598, 630
Sau3AI GATC 1 cut(s) 478
Sau96I GGNCC 1 cut(s) 641
ScaI AGTACT 1 cut(s) 394
SchI GAGTC 2 cut(s) 94, 441
ScrFI CCNGG 4 cut(s) 20, 120, 529, 620
Sfr274I CTCGAG 1 cut(s) 373
SinI GGWCC 1 cut(s) 641
SlaI CTCGAG 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 3 cut(s) 253, 516, 538
SsiI CCGC 6 cut(s) 183, 186, 302, 337, 359, 419
SspMI CTAG 4 cut(s) 234, 282, 579, 661
StyD4I CCNGG 4 cut(s) 18, 118, 527, 618
TaaI ACNGT 2 cut(s) 196, 512
TaqI TCGA 6 cut(s) 374, 389, 415, 430, 543, 575
TasI AATT 3 cut(s) 253, 516, 538
TatI WGTACW 2 cut(s) 57, 392
TauI GCSGC 3 cut(s) 188, 339, 362
TfiI GAWTC 3 cut(s) 237, 272, 285
TscAI CASTG 2 cut(s) 42, 222
TseI GCWGC 3 cut(s) 188, 597, 629
TspDTI ATGAA 3 cut(s) 58, 188, 603
TspRI CASTG 2 cut(s) 42, 222
Tth111I GACNNNGTC 1 cut(s) 315
Van91I CCANNNNNTGG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 641
XagI CCTNNNNNAGG 1 cut(s) 376
XapI RAATTY 1 cut(s) 538
XhoI CTCGAG 1 cut(s) 373
XspI CTAG 4 cut(s) 234, 282, 579, 661
ZrmI AGTACT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.