FvH4_3g28940

anion transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004686
Physical Location & Seq
Forward (+)
22031614 .. 22034105
2492 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28940.t1

Sequence Viewer

Length: 1329 bp
ATGACGATGGTAAAATTTCCCAAGCGCTACTTGATCGTCATATTGACCTTCGTCTGCACGTCCGTTTGCTACATTGAGCGTGTTGGGTTTTCAATTGCATACACTGTTGCTGCTGATGCTGCAGGGGTTAACCAATCAAGTAAAGGCACTATTCTTTCTACGTTCTATTACGGTTATGCTTGTTCACAAGTGCCTGGAGGTTGGGCAGCTCAGAAGATTGGGGGAAGGAAGGTTCTTCTTCTTTCGTTTGTGTTATGGTCAGCTACTTGTGCATTAGTTCCTCTAGATCCCAATCGGATCTTTGTCTTAGTGATTGCTCGGCTGCTTGTTGGTGTTGCACAAGGGTTCATTTTCCCCTCGATCCACACGGTTCTAGCTCAGTGGGTTCCCCCACATGAAAGATCCCGATCTGTTTCTCTTACTACCTCTGGTATGTATCTAGGAGCTGCTACAGGAATGCTTCTCCTTCCAAGCCTTGTAAAGTACAGAGGCCCCCAATCTGTATTTCTTGCGGAAGCAGCATTAGGTGCCATGTGGTCTTTGCTGTGGTTCAAGTATGCCAGTGATCCCCCTTCCTCTCAACATCCAAAAGCAACAGCTGCTGGTTTTGGGGAATCGTTGTTGCCGACCAGAGGAAACCAAAAGTTGAAAGTAGAGAATGGAGGAAGTTCCACTAGAACTGCCAAAATCCCATGGAAGAGAATTTTACTCAGTTTGCCAGTTTGGGCAATCGTGGTTAATAATTTCACGTTCCATTATGCTTTATATGTGTTGATGAACTGGCTGCCAACATACTTTGAGTTAGGCCTCCAACTTAGCCTTCAGGAAATGGGTTCTTCTAAGATGTTGCCGTATCTCAACATGTTTATTTTCTCAAATATAGGTGGGGTGGTAGCTGACTACTTAGTCACCAAGAGAATACTGTCTGTGACTTATACTCGCAAGTTCTTGAACACCGTTGGTTTCATTGTTGCTTCCATTGCATTGATGTCAATTCCCATCTTCCGAACTTCTGATGGGGCAGTCTTTTGTTCATCTGTGGCTCTTGGTTTCTTGGCACTCGGGAGGGCTGGTTTTGCAGTGAATCACATGGATATTGCCCCGAGATATGCTGGTATTGTAATGGGAGTTTCGAACACTGCTGGTACCTTGGCTGGCATTATTGGAGTTGATCTGACTGGCAAGCTTCTTGAAGCTGCTAGAACTGCTGATTCAAGTCTTTCAAGTCCAGAGAGCTGGAGACTGGTGTTTTTAATTCCGGGTCTGCTCTGCATTATCAGTTCTTTTGTATTTTTACTATTCTCAACAGGGGAGAGAATTTTTGACTGA

Protein Analysis

443

Amino Acids

47.98

Weight (kDa)

9.66

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 17 - 389 2.6e-49 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 905
Acc65I GGTACC 1 cut(s) 1145
AccB1I GGYRCC 2 cut(s) 527, 1145
AciI CCGC 1 cut(s) 512
AclWI GGATC 5 cut(s) 281, 305, 355, 396, 560
AcsI RAATTY 3 cut(s) 14, 702, 1317
AcuI CTGAAG 1 cut(s) 806
AfaI GTAC 2 cut(s) 485, 1147
AfeI AGCGCT 1 cut(s) 26
AfiI CCNNNNNNNGG 1 cut(s) 632
AflIII ACRYGT 1 cut(s) 861
AgsI TTSAA 7 cut(s) 93, 553, 649, 952, 1193, 1215, 1224
AjiI CACGTC 1 cut(s) 60
AjnI CCWGG 1 cut(s) 193
AjuI GAANNNNNNNTTGG 4 cut(s) 489, 521, 804, 836
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
AluBI AGCT 9 cut(s) 209, 263, 377, 446, 599, 896, 1186, 1196, 1236
AluI AGCT 9 cut(s) 209, 263, 377, 446, 599, 896, 1186, 1196, 1236
Alw26I GTCTC 1 cut(s) 1234
AlwI GGATC 5 cut(s) 281, 305, 355, 396, 560
AlwNI CAGNNNCTG 1 cut(s) 602
Ama87I CYCGRG 2 cut(s) 1061, 1102
Aor51HI AGCGCT 1 cut(s) 26
AoxI GGCC 2 cut(s) 490, 805
ApeKI GCWGC 9 cut(s) 110, 119, 206, 322, 446, 518, 599, 784, 1196
ApoI RAATTY 3 cut(s) 14, 702, 1317
Asp718I GGTACC 1 cut(s) 1145
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 1 cut(s) 491
AsuC2I CCSGG 1 cut(s) 1260
AsuHPI GGTGA 1 cut(s) 901
AsuII TTCGAA 1 cut(s) 1133
AvaI CYCGRG 2 cut(s) 1061, 1102
BanI GGYRCC 2 cut(s) 527, 1145
BbvI GCAGC 9 cut(s) 97, 106, 218, 309, 433, 530, 586, 771, 1183
BccI CCATC 2 cut(s) 1007, 1010
BceAI ACGGC 1 cut(s) 835
BciT130I CCWGG 1 cut(s) 195
BcnI CCSGG 1 cut(s) 1260
BcoDI GTCTC 1 cut(s) 1234
BfaI CTAG 5 cut(s) 284, 374, 440, 675, 1200
BfmI CTRYAG 2 cut(s) 120, 450
BfoI RGCGCY 1 cut(s) 28
BisI GCNGC 9 cut(s) 111, 120, 207, 323, 447, 519, 600, 785, 1197
BlsI GCNGC 9 cut(s) 112, 121, 208, 324, 448, 520, 601, 786, 1198
Bme1390I CCNGG 2 cut(s) 195, 1260
BmeT110I CYCGRG 2 cut(s) 1061, 1102
BmgBI CACGTC 1 cut(s) 60
BmgT120I GGNCC 1 cut(s) 491
BmiI GGNNCC 4 cut(s) 387, 493, 529, 1147
BmrFI CCNGG 2 cut(s) 195, 1260
BmsI GCATC 1 cut(s) 106
BoxI GACNNNNGTC 1 cut(s) 50
BpmI CTGGAG 2 cut(s) 216, 1258
Bpu14I TTCGAA 1 cut(s) 1133
BpuMI CCSGG 1 cut(s) 1260
BsaBI GATNNNNATC 2 cut(s) 291, 406
BsaJI CCNNGG 2 cut(s) 692, 1149
BsaXI ACNNNNNCTCC 2 cut(s) 1057, 1087
Bsc4I CCNNNNNNNGG 1 cut(s) 632
Bse1I ACTGG 5 cut(s) 561, 719, 785, 1183, 1248
Bse3DI GCAATG 1 cut(s) 978
Bse8I GATNNNNATC 2 cut(s) 291, 406
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 2 cut(s) 692, 1149
BseGI GGATG 1 cut(s) 583
BseJI GATNNNNATC 2 cut(s) 291, 406
BseLI CCNNNNNNNGG 1 cut(s) 632
BseMI GCAATG 1 cut(s) 978
BseMII CTCAG 3 cut(s) 224, 392, 724
BseNI ACTGG 5 cut(s) 561, 719, 785, 1183, 1248
BseXI GCAGC 9 cut(s) 97, 106, 218, 309, 433, 530, 586, 771, 1183
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 2 cut(s) 492, 807
BshNI GGYRCC 2 cut(s) 527, 1145
BsiHKCI CYCGRG 2 cut(s) 1061, 1102
BsiSI CCGG 1 cut(s) 1259
BslI CCNNNNNNNGG 1 cut(s) 632
BsmAI GTCTC 1 cut(s) 1234
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 2 cut(s) 492, 807
BsoBI CYCGRG 2 cut(s) 1061, 1102
Bsp119I TTCGAA 1 cut(s) 1133
Bsp143I GATC 8 cut(s) 33, 286, 297, 360, 401, 407, 565, 1171
Bsp19I CCATGG 1 cut(s) 692
BspACI CCGC 1 cut(s) 512
BspANI GGCC 2 cut(s) 492, 807
BspCNI CTCAG 3 cut(s) 223, 391, 723
BspLI GGNNCC 4 cut(s) 387, 493, 529, 1147
BspMAI CTGCAG 1 cut(s) 124
BspPI GGATC 5 cut(s) 281, 305, 355, 396, 560
BspT104I TTCGAA 1 cut(s) 1133
BspT107I GGYRCC 2 cut(s) 527, 1145
BsrDI GCAATG 1 cut(s) 978
BsrI ACTGG 5 cut(s) 561, 719, 785, 1183, 1248
BssECI CCNNGG 2 cut(s) 692, 1149
BssMI GATC 8 cut(s) 33, 286, 297, 360, 401, 407, 565, 1171
BssT1I CCWWGG 2 cut(s) 692, 1149
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 5 cut(s) 106, 173, 370, 924, 958
Bst6I CTCTTC 1 cut(s) 692
BstAPI GCANNNNNTGC 2 cut(s) 527, 599
BstBI TTCGAA 1 cut(s) 1133
BstC8I GCNNGC 2 cut(s) 1156, 1184
BstDEI CTNAG 7 cut(s) 210, 307, 378, 710, 815, 840, 904
BstDSI CCRYGG 1 cut(s) 692
BstF5I GGATG 1 cut(s) 583
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 8 cut(s) 36, 289, 300, 363, 404, 410, 568, 1174
BstMAI GTCTC 1 cut(s) 1234
BstMBI GATC 8 cut(s) 33, 286, 297, 360, 401, 407, 565, 1171
BstMWI GCNNNNNNNGC 9 cut(s) 116, 119, 269, 518, 527, 599, 980, 1076, 1205
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 865
BstPAI GACNNNNGTC 1 cut(s) 50
BstSCI CCNGG 2 cut(s) 193, 1258
BstSFI CTRYAG 2 cut(s) 120, 450
BstV1I GCAGC 9 cut(s) 97, 106, 218, 309, 433, 530, 586, 771, 1183
BstX2I RGATCY 3 cut(s) 286, 297, 401
BstXI CCANNNNNNTGG 1 cut(s) 1236
BstYI RGATCY 3 cut(s) 286, 297, 401
BsuRI GGCC 2 cut(s) 492, 807
BtgI CCRYGG 1 cut(s) 692
BtrI CACGTC 1 cut(s) 60
BtsCI GGATG 1 cut(s) 583
BtsI GCAGTG 2 cut(s) 1086, 1137
BtsIMutI CAGTG 5 cut(s) 102, 386, 568, 1086, 1137
Cac8I GCNNGC 2 cut(s) 1156, 1184
CaiI CAGNNNCTG 1 cut(s) 602
CfoI GCGC 1 cut(s) 27
Cfr13I GGNCC 1 cut(s) 491
Csp6I GTAC 2 cut(s) 484, 1146
CviAII CATG 5 cut(s) 395, 532, 693, 862, 1090
CviQI GTAC 2 cut(s) 484, 1146
DdeI CTNAG 7 cut(s) 210, 307, 378, 710, 815, 840, 904
DpnI GATC 8 cut(s) 35, 288, 299, 362, 403, 409, 567, 1173
DpnII GATC 8 cut(s) 33, 286, 297, 360, 401, 407, 565, 1171
DrdI GACNNNNNNGTC 1 cut(s) 905
DseDI GACNNNNNNGTC 1 cut(s) 905
Eam1104I CTCTTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 692
Eco130I CCWWGG 2 cut(s) 692, 1149
Eco147I AGGCCT 1 cut(s) 807
Eco47III AGCGCT 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 806
Eco88I CYCGRG 2 cut(s) 1061, 1102
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 692, 1149
ErhI CCWWGG 2 cut(s) 692, 1149
FaeI CATG 5 cut(s) 398, 535, 696, 865, 1093
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FatI CATG 5 cut(s) 394, 531, 692, 861, 1089
Fnu4HI GCNGC 9 cut(s) 111, 120, 207, 323, 447, 519, 600, 785, 1197
FokI GGATG 1 cut(s) 570
Fsp4HI GCNGC 9 cut(s) 111, 120, 207, 323, 447, 519, 600, 785, 1197
FspBI CTAG 5 cut(s) 284, 374, 440, 675, 1200
GlaI GCGC 1 cut(s) 26
GluI GCNGC 9 cut(s) 111, 120, 207, 323, 447, 519, 600, 785, 1197
GsuI CTGGAG 2 cut(s) 216, 1258
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 2 cut(s) 492, 807
HapII CCGG 1 cut(s) 1259
HhaI GCGC 1 cut(s) 27
Hin1II CATG 5 cut(s) 398, 535, 696, 865, 1093
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 1184
HinfI GANTC 3 cut(s) 614, 1084, 1211
HpaI GTTAAC 1 cut(s) 130
HpaII CCGG 1 cut(s) 1259
HphI GGTGA 1 cut(s) 901
Hpy166II GTNNAC 2 cut(s) 130, 185
Hpy188I TCNGA 5 cut(s) 213, 297, 1007, 1015, 1176
Hpy188III TCNNGA 7 cut(s) 284, 405, 824, 949, 1063, 1190, 1229
Hpy8I GTNNAC 2 cut(s) 130, 185
HpyAV CCTTC 6 cut(s) 58, 219, 223, 476, 582, 830
HpyCH4III ACNGT 5 cut(s) 106, 173, 370, 924, 958
HpyCH4IV ACGT 3 cut(s) 59, 161, 749
HpyCH4V TGCA 8 cut(s) 57, 98, 122, 272, 338, 983, 1079, 1272
HpyF10VI GCNNNNNNNGC 9 cut(s) 116, 119, 269, 518, 527, 599, 980, 1076, 1205
HpyF3I CTNAG 7 cut(s) 210, 307, 378, 710, 815, 840, 904
HpySE526I ACGT 3 cut(s) 59, 161, 749
Hsp92II CATG 5 cut(s) 398, 535, 696, 865, 1093
HspAI GCGC 1 cut(s) 25
KpnI GGTACC 1 cut(s) 1149
KspAI GTTAAC 1 cut(s) 130
Kzo9I GATC 8 cut(s) 33, 286, 297, 360, 401, 407, 565, 1171
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 9 cut(s) 97, 106, 218, 309, 433, 530, 586, 771, 1183
LweI GCATC 1 cut(s) 106
MaeI CTAG 5 cut(s) 284, 374, 440, 675, 1200
MaeII ACGT 3 cut(s) 59, 161, 749
MaeIII GTNAC 2 cut(s) 907, 928
MalI GATC 8 cut(s) 35, 288, 299, 362, 403, 409, 567, 1173
MboI GATC 8 cut(s) 33, 286, 297, 360, 401, 407, 565, 1171
MboII GAAGA 6 cut(s) 226, 227, 230, 709, 828, 994
MfeI CAATTG 1 cut(s) 93
MflI RGATCY 3 cut(s) 286, 297, 401
MluCI AATT 7 cut(s) 14, 93, 702, 742, 993, 1254, 1317
MmeI TCCRAC 1 cut(s) 835
MseI TTAA 3 cut(s) 129, 738, 1253
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 1 cut(s) 1259
MspR9I CCNGG 2 cut(s) 195, 1260
MunI CAATTG 1 cut(s) 93
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 9 cut(s) 116, 119, 269, 518, 527, 599, 980, 1076, 1205
NciI CCSGG 1 cut(s) 1260
NcoI CCATGG 1 cut(s) 692
NdeII GATC 8 cut(s) 33, 286, 297, 360, 401, 407, 565, 1171
NlaIII CATG 5 cut(s) 398, 535, 696, 865, 1093
NlaIV GGNNCC 4 cut(s) 387, 493, 529, 1147
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 2 cut(s) 907, 928
NspI RCATGY 1 cut(s) 865
NspV TTCGAA 1 cut(s) 1133
PceI AGGCCT 1 cut(s) 807
PciI ACATGT 1 cut(s) 861
PcsI WCGNNNNNNNCGW 1 cut(s) 623
PctI GAATGC 1 cut(s) 462
PfeI GAWTC 3 cut(s) 614, 1084, 1211
PkrI GCNGC 9 cut(s) 112, 121, 208, 324, 448, 520, 601, 786, 1198
PscI ACATGT 1 cut(s) 861
PshAI GACNNNNGTC 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 4 cut(s) 387, 493, 529, 1147
PspPI GGNCC 1 cut(s) 491
PstI CTGCAG 1 cut(s) 124
PstNI CAGNNNCTG 1 cut(s) 602
PsuI RGATCY 3 cut(s) 286, 297, 401
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 2 cut(s) 485, 1147
RsaNI GTAC 2 cut(s) 484, 1146
SaqAI TTAA 3 cut(s) 129, 738, 1253
SatI GCNGC 9 cut(s) 111, 120, 207, 323, 447, 519, 600, 785, 1197
Sau3AI GATC 8 cut(s) 33, 286, 297, 360, 401, 407, 565, 1171
Sau96I GGNCC 1 cut(s) 491
ScrFI CCNGG 2 cut(s) 195, 1260
SfaNI GCATC 1 cut(s) 106
SfcI CTRYAG 2 cut(s) 120, 450
SfuI TTCGAA 1 cut(s) 1133
Sse9I AATT 7 cut(s) 14, 93, 702, 742, 993, 1254, 1317
SseBI AGGCCT 1 cut(s) 807
SsiI CCGC 1 cut(s) 512
SspMI CTAG 5 cut(s) 284, 374, 440, 675, 1200
StuI AGGCCT 1 cut(s) 807
StyD4I CCNGG 2 cut(s) 193, 1258
StyI CCWWGG 2 cut(s) 692, 1149
TaaI ACNGT 5 cut(s) 106, 173, 370, 924, 958
TaiI ACGT 3 cut(s) 62, 164, 752
TaqI TCGA 2 cut(s) 359, 1133
TasI AATT 7 cut(s) 14, 93, 702, 742, 993, 1254, 1317
TatI WGTACW 1 cut(s) 483
TfiI GAWTC 3 cut(s) 614, 1084, 1211
Tru1I TTAA 3 cut(s) 129, 738, 1253
Tru9I TTAA 3 cut(s) 129, 738, 1253
TscAI CASTG 5 cut(s) 109, 386, 568, 1086, 1144
TseFI GTSAC 2 cut(s) 907, 928
TseI GCWGC 9 cut(s) 110, 119, 206, 322, 446, 518, 599, 784, 1196
Tsp45I GTSAC 2 cut(s) 907, 928
TspDTI ATGAA 5 cut(s) 337, 411, 791, 955, 1023
TspGWI ACGGA 1 cut(s) 52
TspRI CASTG 5 cut(s) 109, 386, 568, 1086, 1144
XapI RAATTY 3 cut(s) 14, 702, 1317
XbaI TCTAGA 1 cut(s) 283
XceI RCATGY 1 cut(s) 865
XspI CTAG 5 cut(s) 284, 374, 440, 675, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.