RchiOBHm_Chr5g0051801

anion transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004686
Physical Location & Seq
Reverse (-)
53166140 .. 53168301
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32925

Sequence Viewer

Length: 1281 bp
ATGTCACTAGAGGTGAAGTATGACGATGAACGGGTCGGGTTTTCGATTGCATATACTGTTGCTGCCGATGCTGCAGGGGTAAACCAATCGAGTAAAGGCACAATTCTTTCTACATTCTATTATGGTTATGCTTGTTCACAAGTACCTGGAGGTTGGGCAGCTCAGAAAATTGGGGGAAGGAAGGTTCTTCTTCTTTCATTTGTGTTATGGTCAGCTACGTGTGCATTAGTTCCTCTAGACCCCAATCGAATCTTTGTCTTGGTGATTGCTCGGTTGCTTGTGGGCGTTGCACAAGGATTCATTTTCCCCTCAATCCACACAGTTCTAGCTCAGTGGGTTCCACCACATGAAAGATCCAGATCTGTTTCTCTTACTACTTCTGGTATGTACCTAGGAGCAGCTGCAGGAATGCTTCTCCTTCCAAGCCTTGTAAAGTACAGGGGCCCCCAATCCGTATTTCTTGCTGAGGCAGCATTAGGTGCCATGTGGTCTTTGCTGTGGTTCAAGTATGCCAGTGATCCACCTTCCTCTCAACATCCAAAAGCAACAGCTGCTGGTTTTGGGGAATCTTTGTTGCCGACCAAAGGAAACCAAAAGTTGAAAGTAGAGAATGGAGGAAGTTCCACTAGAACTGCCAAGATCCCATGGAAGAGAATTTTACTCAGTTTGCCAGTTTGGGCAATCGTGGTTAATAATTTCACGTTCCATTATGCTTTATACGTGTTGATGAACTGGCTGCCCACATACTTTGAATTAGGCCTCCAACTTAGCCTTCAGGAAATGGGTTCTTCTAAGATGTTGCCGTATCTCAACATGTTTATTTTCTCAAATATAGGTGGGGTGGTAGCTGACCATTTAGTCACCAAGAGAATATTGTCTGTGACTAATACTCGCAAGTTCTTGAACACCATTGGTTTCATAGTTGCTTCTCTTGCGTTGATGGCAATTCCCATCTTCCGAACTTCTGCTGGGGCTGTCTTTTGTTCTTCTGTAGCTCTTGGTTTCTTGGCACTCGGGAGGGCTGGTTTTGCAGTGAATCACATGGATATTGCCCCGAGATATGCTGGTATTGTAATGGGAGTTTCAAACACTGCTGGTACCTTAGCTGGCATTATTGGAGTTGATCTGACTGGCAAGCTTCTTGAAGCTGCAAGAACTGCTGATTCGAGTCTTTCAAGTCCAGAGAGCTGGAGACTGGTGTTTTTAATTCCGGGTCTGCTCTGCGTTTTCAGTTCTTTTGTATTTTTACTATTCTCAACCGGGGAGAGAATTTTTGACTGA

Protein Analysis

426

Amino Acids

45.95

Weight (kDa)

9.57

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 14 - 373 6.8e-49 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 857
Acc65I GGTACC 1 cut(s) 1097
AccB1I GGYRCC 2 cut(s) 479, 1097
AclWI GGATC 3 cut(s) 348, 512, 634
AcsI RAATTY 2 cut(s) 654, 1269
AcuI CTGAAG 1 cut(s) 758
AfaI GTAC 4 cut(s) 144, 389, 437, 1099
AfiI CCNNNNNNNGG 1 cut(s) 584
AflIII ACRYGT 3 cut(s) 218, 720, 813
AgsI TTSAA 7 cut(s) 505, 601, 752, 904, 1086, 1145, 1176
AjnI CCWGG 1 cut(s) 145
AjuI GAANNNNNNNTTGG 4 cut(s) 441, 473, 756, 788
AloI GAACNNNNNNTCC 2 cut(s) 168, 200
Alw26I GTCTC 1 cut(s) 1186
AlwI GGATC 3 cut(s) 348, 512, 634
AlwNI CAGNNNCTG 1 cut(s) 554
Ama87I CYCGRG 2 cut(s) 1013, 1054
AoxI GGCC 2 cut(s) 442, 757
ApaI GGGCCC 1 cut(s) 446
ApeKI GCWGC 9 cut(s) 62, 71, 158, 398, 401, 470, 551, 736, 1148
ApoI RAATTY 2 cut(s) 654, 1269
Asp718I GGTACC 1 cut(s) 1097
AspA2I CCTAGG 1 cut(s) 391
AspS9I GGNCC 2 cut(s) 442, 443
AsuC2I CCSGG 2 cut(s) 1212, 1261
AsuHPI GGTGA 3 cut(s) 25, 274, 853
AvaI CYCGRG 2 cut(s) 1013, 1054
AvrII CCTAGG 1 cut(s) 391
BaeGI GKGCMC 1 cut(s) 446
BanI GGYRCC 2 cut(s) 479, 1097
BanII GRGCYC 1 cut(s) 446
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 9 cut(s) 49, 58, 170, 388, 410, 482, 538, 723, 1135
BccI CCATC 2 cut(s) 934, 959
BceAI ACGGC 1 cut(s) 787
BciT130I CCWGG 1 cut(s) 147
BcnI CCSGG 2 cut(s) 1212, 1261
BcoDI GTCTC 1 cut(s) 1186
BfaI CTAG 5 cut(s) 8, 236, 326, 392, 627
BfmI CTRYAG 3 cut(s) 72, 402, 990
BglII AGATCT 1 cut(s) 359
BisI GCNGC 9 cut(s) 63, 72, 159, 399, 402, 471, 552, 737, 1149
BlnI CCTAGG 1 cut(s) 391
BlsI GCNGC 9 cut(s) 64, 73, 160, 400, 403, 472, 553, 738, 1150
Bme1390I CCNGG 3 cut(s) 147, 1212, 1261
BmeT110I CYCGRG 2 cut(s) 1013, 1054
BmgT120I GGNCC 2 cut(s) 442, 443
BmiI GGNNCC 6 cut(s) 339, 443, 444, 445, 481, 1099
BmrFI CCNGG 3 cut(s) 147, 1212, 1261
BmsI GCATC 1 cut(s) 58
BpmI CTGGAG 2 cut(s) 168, 1210
Bpu10I CCTNAGC 2 cut(s) 465, 1102
BpuMI CCSGG 2 cut(s) 1212, 1261
BsaAI YACGTR 2 cut(s) 219, 721
BsaBI GATNNNNATC 1 cut(s) 358
BsaJI CCNNGG 3 cut(s) 391, 644, 1260
BsaXI ACNNNNNCTCC 2 cut(s) 1009, 1039
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse1I ACTGG 5 cut(s) 513, 671, 737, 1135, 1200
Bse8I GATNNNNATC 1 cut(s) 358
BseBI CCWGG 1 cut(s) 147
BseDI CCNNGG 3 cut(s) 391, 644, 1260
BseGI GGATG 1 cut(s) 535
BseJI GATNNNNATC 1 cut(s) 358
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMII CTCAG 4 cut(s) 176, 344, 456, 676
BseNI ACTGG 5 cut(s) 513, 671, 737, 1135, 1200
BseSI GKGCMC 1 cut(s) 446
BseXI GCAGC 9 cut(s) 49, 58, 170, 388, 410, 482, 538, 723, 1135
BseYI CCCAGC 1 cut(s) 968
BshFI GGCC 2 cut(s) 444, 759
BshNI GGYRCC 2 cut(s) 479, 1097
BsiHKCI CYCGRG 2 cut(s) 1013, 1054
BsiSI CCGG 2 cut(s) 1211, 1260
BslI CCNNNNNNNGG 1 cut(s) 584
BsmAI GTCTC 1 cut(s) 1186
BsmI GAATGC 1 cut(s) 414
BsnI GGCC 2 cut(s) 444, 759
BsoBI CYCGRG 2 cut(s) 1013, 1054
Bsp120I GGGCCC 1 cut(s) 442
Bsp1286I GDGCHC 1 cut(s) 446
Bsp143I GATC 5 cut(s) 353, 359, 517, 639, 1123
Bsp19I CCATGG 1 cut(s) 644
BspANI GGCC 2 cut(s) 444, 759
BspCNI CTCAG 4 cut(s) 175, 343, 457, 675
BspLI GGNNCC 6 cut(s) 339, 443, 444, 445, 481, 1099
BspMAI CTGCAG 2 cut(s) 76, 406
BspPI GGATC 3 cut(s) 348, 512, 634
BspT107I GGYRCC 2 cut(s) 479, 1097
BsrI ACTGG 5 cut(s) 513, 671, 737, 1135, 1200
BssECI CCNNGG 3 cut(s) 391, 644, 1260
BssMI GATC 5 cut(s) 353, 359, 517, 639, 1123
BssT1I CCWWGG 2 cut(s) 391, 644
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 58, 322
Bst6I CTCTTC 1 cut(s) 644
BstAPI GCANNNNNTGC 3 cut(s) 479, 551, 1157
BstBAI YACGTR 2 cut(s) 219, 721
BstC8I GCNNGC 2 cut(s) 1108, 1136
BstDEI CTNAG 7 cut(s) 162, 330, 465, 662, 767, 792, 1102
BstDSI CCRYGG 1 cut(s) 644
BstF5I GGATG 1 cut(s) 535
BstKTI GATC 5 cut(s) 356, 362, 520, 642, 1126
BstMAI GTCTC 1 cut(s) 1186
BstMBI GATC 5 cut(s) 353, 359, 517, 639, 1123
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 1 cut(s) 817
BstSCI CCNGG 3 cut(s) 145, 1210, 1259
BstSFI CTRYAG 3 cut(s) 72, 402, 990
BstSLI GKGCMC 1 cut(s) 446
BstV1I GCAGC 9 cut(s) 49, 58, 170, 388, 410, 482, 538, 723, 1135
BstX2I RGATCY 3 cut(s) 353, 359, 639
BstXI CCANNNNNNTGG 1 cut(s) 1188
BstYI RGATCY 3 cut(s) 353, 359, 639
BsuRI GGCC 2 cut(s) 444, 759
BtgI CCRYGG 1 cut(s) 644
BtsCI GGATG 1 cut(s) 535
BtsI GCAGTG 2 cut(s) 1038, 1089
BtsIMutI CAGTG 4 cut(s) 338, 520, 1038, 1089
Cac8I GCNNGC 2 cut(s) 1108, 1136
CaiI CAGNNNCTG 1 cut(s) 554
Cfr13I GGNCC 2 cut(s) 442, 443
Csp6I GTAC 4 cut(s) 143, 388, 436, 1098
CspCI CAANNNNNGTGG 2 cut(s) 730, 765
CviAII CATG 5 cut(s) 347, 484, 645, 814, 1042
CviQI GTAC 4 cut(s) 143, 388, 436, 1098
DdeI CTNAG 7 cut(s) 162, 330, 465, 662, 767, 792, 1102
DpnI GATC 5 cut(s) 355, 361, 519, 641, 1125
DpnII GATC 5 cut(s) 353, 359, 517, 639, 1123
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
Eam1104I CTCTTC 1 cut(s) 644
EarI CTCTTC 1 cut(s) 644
Eco130I CCWWGG 2 cut(s) 391, 644
Eco147I AGGCCT 1 cut(s) 759
Eco24I GRGCYC 1 cut(s) 446
Eco57I CTGAAG 1 cut(s) 758
Eco88I CYCGRG 2 cut(s) 1013, 1054
EcoO109I RGGNCCY 2 cut(s) 442, 443
EcoRII CCWGG 1 cut(s) 145
EcoT14I CCWWGG 2 cut(s) 391, 644
EcoT38I GRGCYC 1 cut(s) 446
ErhI CCWWGG 2 cut(s) 391, 644
FaeI CATG 5 cut(s) 350, 487, 648, 817, 1045
FatI CATG 5 cut(s) 346, 483, 644, 813, 1041
Fnu4HI GCNGC 9 cut(s) 63, 72, 159, 399, 402, 471, 552, 737, 1149
FokI GGATG 1 cut(s) 522
FriOI GRGCYC 1 cut(s) 446
Fsp4HI GCNGC 9 cut(s) 63, 72, 159, 399, 402, 471, 552, 737, 1149
FspBI CTAG 5 cut(s) 8, 236, 326, 392, 627
GluI GCNGC 9 cut(s) 63, 72, 159, 399, 402, 471, 552, 737, 1149
GsaI CCCAGC 1 cut(s) 972
GsuI CTGGAG 2 cut(s) 168, 1210
HaeIII GGCC 2 cut(s) 444, 759
HapII CCGG 2 cut(s) 1211, 1260
Hin1II CATG 5 cut(s) 350, 487, 648, 817, 1045
HindIII AAGCTT 1 cut(s) 1136
HinfI GANTC 6 cut(s) 249, 297, 566, 1036, 1163, 1168
HpaII CCGG 2 cut(s) 1211, 1260
HphI GGTGA 3 cut(s) 25, 274, 853
Hpy166II GTNNAC 2 cut(s) 82, 137
Hpy188I TCNGA 3 cut(s) 165, 959, 1128
Hpy188III TCNNGA 7 cut(s) 236, 357, 776, 901, 1015, 1142, 1181
Hpy8I GTNNAC 2 cut(s) 82, 137
HpyAV CCTTC 5 cut(s) 171, 175, 428, 534, 782
HpyCH4III ACNGT 2 cut(s) 58, 322
HpyCH4IV ACGT 3 cut(s) 218, 701, 720
HpyCH4V TGCA 7 cut(s) 50, 74, 224, 290, 404, 1031, 1151
HpyF3I CTNAG 7 cut(s) 162, 330, 465, 662, 767, 792, 1102
HpySE526I ACGT 3 cut(s) 218, 701, 720
Hsp92II CATG 5 cut(s) 350, 487, 648, 817, 1045
KpnI GGTACC 1 cut(s) 1101
Kzo9I GATC 5 cut(s) 353, 359, 517, 639, 1123
LmnI GCTCC 1 cut(s) 395
Lsp1109I GCAGC 9 cut(s) 49, 58, 170, 388, 410, 482, 538, 723, 1135
LweI GCATC 1 cut(s) 58
MaeI CTAG 5 cut(s) 8, 236, 326, 392, 627
MaeII ACGT 3 cut(s) 218, 701, 720
MaeIII GTNAC 3 cut(s) 3, 859, 880
MalI GATC 5 cut(s) 355, 361, 519, 641, 1125
MboI GATC 5 cut(s) 353, 359, 517, 639, 1123
MboII GAAGA 6 cut(s) 179, 182, 661, 780, 946, 978
MflI RGATCY 3 cut(s) 353, 359, 639
MhlI GDGCHC 1 cut(s) 446
MluCI AATT 8 cut(s) 102, 168, 654, 694, 752, 945, 1206, 1269
MlyI GAGTC 1 cut(s) 1177
MmeI TCCRAC 1 cut(s) 787
MnlI CCTC 9 cut(s) 4, 143, 243, 319, 460, 538, 608, 770, 1011
MseI TTAA 2 cut(s) 690, 1205
MspA1I CMGCKG 2 cut(s) 401, 551
MspI CCGG 2 cut(s) 1211, 1260
MspR9I CCNGG 3 cut(s) 147, 1212, 1261
Mva1269I GAATGC 1 cut(s) 414
MvaI CCWGG 1 cut(s) 147
NciI CCSGG 2 cut(s) 1212, 1261
NcoI CCATGG 1 cut(s) 644
NdeII GATC 5 cut(s) 353, 359, 517, 639, 1123
NlaIII CATG 5 cut(s) 350, 487, 648, 817, 1045
NlaIV GGNNCC 6 cut(s) 339, 443, 444, 445, 481, 1099
NmuCI GTSAC 3 cut(s) 3, 859, 880
NspI RCATGY 1 cut(s) 817
PceI AGGCCT 1 cut(s) 759
PciI ACATGT 1 cut(s) 813
PctI GAATGC 1 cut(s) 414
PfeI GAWTC 5 cut(s) 249, 297, 566, 1036, 1163
PkrI GCNGC 9 cut(s) 64, 73, 160, 400, 403, 472, 553, 738, 1150
PleI GAGTC 1 cut(s) 1176
PpsI GAGTC 1 cut(s) 1176
Ppu21I YACGTR 2 cut(s) 219, 721
PscI ACATGT 1 cut(s) 813
Psp6I CCWGG 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 968
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 6 cut(s) 339, 443, 444, 445, 481, 1099
PspOMI GGGCCC 1 cut(s) 442
PspPI GGNCC 2 cut(s) 442, 443
PstI CTGCAG 2 cut(s) 76, 406
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 3 cut(s) 353, 359, 639
PvuII CAGCTG 2 cut(s) 401, 551
RsaI GTAC 4 cut(s) 144, 389, 437, 1099
RsaNI GTAC 4 cut(s) 143, 388, 436, 1098
SaqAI TTAA 2 cut(s) 690, 1205
SatI GCNGC 9 cut(s) 63, 72, 159, 399, 402, 471, 552, 737, 1149
Sau3AI GATC 5 cut(s) 353, 359, 517, 639, 1123
Sau96I GGNCC 2 cut(s) 442, 443
SchI GAGTC 1 cut(s) 1177
ScrFI CCNGG 3 cut(s) 147, 1212, 1261
SduI GDGCHC 1 cut(s) 446
SfaNI GCATC 1 cut(s) 58
SfcI CTRYAG 3 cut(s) 72, 402, 990
Sse9I AATT 8 cut(s) 102, 168, 654, 694, 752, 945, 1206, 1269
SseBI AGGCCT 1 cut(s) 759
SspI AATATT 1 cut(s) 873
SspMI CTAG 5 cut(s) 8, 236, 326, 392, 627
StuI AGGCCT 1 cut(s) 759
StyD4I CCNGG 3 cut(s) 145, 1210, 1259
StyI CCWWGG 2 cut(s) 391, 644
TaaI ACNGT 2 cut(s) 58, 322
TaiI ACGT 3 cut(s) 221, 704, 723
TaqI TCGA 4 cut(s) 44, 89, 247, 1166
TasI AATT 8 cut(s) 102, 168, 654, 694, 752, 945, 1206, 1269
TatI WGTACW 1 cut(s) 435
TfiI GAWTC 5 cut(s) 249, 297, 566, 1036, 1163
Tru1I TTAA 2 cut(s) 690, 1205
Tru9I TTAA 2 cut(s) 690, 1205
TscAI CASTG 4 cut(s) 338, 520, 1038, 1096
TseFI GTSAC 3 cut(s) 3, 859, 880
TseI GCWGC 9 cut(s) 62, 71, 158, 398, 401, 470, 551, 736, 1148
Tsp45I GTSAC 3 cut(s) 3, 859, 880
TspDTI ATGAA 6 cut(s) 42, 186, 289, 363, 743, 907
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 4 cut(s) 338, 520, 1038, 1096
XapI RAATTY 2 cut(s) 654, 1269
XbaI TCTAGA 1 cut(s) 235
XceI RCATGY 1 cut(s) 817
XmaJI CCTAGG 1 cut(s) 391
XspI CTAG 5 cut(s) 8, 236, 326, 392, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.