Rw5G032920

anion transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004686
Physical Location & Seq
Forward (+)
55885304 .. 55886630
1327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032920.1

Sequence Viewer

Length: 1269 bp
ATGACGATGGTGAAATTTCCTAAGCGCTACTTAATCGTCATATTGACCTTCGTCTGCACGTCTGTTTGCTATATAGAACGGGTTGGGTTTTCGATTGCATATACTGTTGCTGCCGATGCTGCAGGGGTGAACCAATCGAGTAAAGGCACAATTCTTTCTACATTCTATTATGGTTACGCTTGTTCTCAAGTACCTGGAGGTTGGGCAGCTCAGAAAATTGGGGGAAGGAAGGTTCTTCTTCTTTCATTTGTGTTATGGTCAGCTACGTGTGCGTTGGTTCCTCTAGACCCCAATCGAATCTTTGTCTTGGTGATTGCTCGGTTGCTTGTGGGTGTTGCACAAGGATTCATTTTCCCCTCAATCCACACGGTTCTAGCTCAGTGGGTTCCACCACATGAAAGATCCAGATCTGTTTCTCTTACTACTTCTGGTATGTACCTAGGAGCAGCTGCAGGAATGCTTCTCCTTCCAAGCCTTGTAAAGTACAGGGGCCCCCAATCCGTATTTCTTGCGGAGGCAGCATTAGGTGCCATTGATCCACCTTCCTCTCAACATCCAAAGGCAACAGCTGCTGGTTTTGGGGAATCTTTGTTGCCGACCAAAGGAAACCAAAAGTTGAAAGTAGAGAATGGAGGAAGTTCCACTAGAACTGCCAAGATCCCATGGAAGAGAATTTTACTCAGTTTGCCGGTTTGGGCAATCGTGGTTAATAATTTCACGTTCCATTATGCCTTATATGTGTTGATGAACTGGCTGCCCACATACTTTGAATTAGGCCTCCAACTTAGCCTTCAGGAAATGGGTTCTTCTAAGATGTTGCCGTATCTCAACATGTTTATTTTCTCAAATATAGGTGGAATATTGTCTGTGACTAATACTCGCAAGTTCTTGAACACCATTGGTTTCATAGTTGCTTCTCTTGCGTTGATGGCAATTCCCATCTTCCGAACTTCTGCTGGGGCTGTCTTTTGTTCTTCTGTAGCTCTTGGTTTCTTGGCACTCGGGAGGGCTGGTTTTGCAGTGAATCACATGGATATTGCCCCGAGATATGCTGGTATTGTAATGGGAGTTTCAAACACTGCTGGTACCTTAGCTGGCATTATTGGAGTTGATCTGACTGGCAAGCTTCTTGAAGCTGCAAGAACTGCTGATTCGAGTCTTTCAAGTCCAGAGAGCTGGAGACTGGTGTTTTTAATTCCGGGTCTGCTCTGCGTTTTCAGTTCTTTTGTATTTTTACTATTCTCAACCGGGGAGAGAATTTTTGACTGA

Protein Analysis

422

Amino Acids

45.4

Weight (kDa)

9.67

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 17 - 369 3e-45 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1085
AccB1I GGYRCC 2 cut(s) 527, 1085
AciI CCGC 1 cut(s) 512
AclWI GGATC 3 cut(s) 396, 530, 652
AcsI RAATTY 3 cut(s) 14, 672, 1257
AcuI CTGAAG 1 cut(s) 776
AfaI GTAC 4 cut(s) 192, 437, 485, 1087
AfeI AGCGCT 1 cut(s) 26
AfiI CCNNNNNNNGG 1 cut(s) 602
AflIII ACRYGT 2 cut(s) 266, 831
AgsI TTSAA 6 cut(s) 619, 770, 892, 1074, 1133, 1164
AjiI CACGTC 1 cut(s) 60
AjnI CCWGG 1 cut(s) 193
AjuI GAANNNNNNNTTGG 4 cut(s) 489, 521, 774, 806
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
Alw26I GTCTC 1 cut(s) 1174
AlwI GGATC 3 cut(s) 396, 530, 652
AlwNI CAGNNNCTG 1 cut(s) 572
Ama87I CYCGRG 2 cut(s) 1001, 1042
Aor51HI AGCGCT 1 cut(s) 26
AoxI GGCC 2 cut(s) 490, 775
ApaI GGGCCC 1 cut(s) 494
ApeKI GCWGC 9 cut(s) 110, 119, 206, 446, 449, 518, 569, 754, 1136
ApoI RAATTY 3 cut(s) 14, 672, 1257
Asp718I GGTACC 1 cut(s) 1085
AspA2I CCTAGG 1 cut(s) 439
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 2 cut(s) 490, 491
AsuC2I CCSGG 2 cut(s) 1200, 1249
AsuHPI GGTGA 3 cut(s) 22, 139, 322
AvaI CYCGRG 2 cut(s) 1001, 1042
AvrII CCTAGG 1 cut(s) 439
BaeGI GKGCMC 1 cut(s) 494
BanI GGYRCC 2 cut(s) 527, 1085
BanII GRGCYC 1 cut(s) 494
BbvI GCAGC 9 cut(s) 97, 106, 218, 436, 458, 530, 556, 741, 1123
BccI CCATC 2 cut(s) 922, 947
BceAI ACGGC 1 cut(s) 805
BciT130I CCWGG 1 cut(s) 195
BcnI CCSGG 2 cut(s) 1200, 1249
BcoDI GTCTC 1 cut(s) 1174
BfaI CTAG 4 cut(s) 284, 374, 440, 645
BfmI CTRYAG 3 cut(s) 120, 450, 978
BfoI RGCGCY 1 cut(s) 28
BglII AGATCT 1 cut(s) 407
BisI GCNGC 9 cut(s) 111, 120, 207, 447, 450, 519, 570, 755, 1137
BlnI CCTAGG 1 cut(s) 439
BlsI GCNGC 9 cut(s) 112, 121, 208, 448, 451, 520, 571, 756, 1138
Bme1390I CCNGG 3 cut(s) 195, 1200, 1249
BmeT110I CYCGRG 2 cut(s) 1001, 1042
BmgBI CACGTC 1 cut(s) 60
BmgT120I GGNCC 2 cut(s) 490, 491
BmiI GGNNCC 7 cut(s) 279, 387, 491, 492, 493, 529, 1087
BmrFI CCNGG 3 cut(s) 195, 1200, 1249
BmsI GCATC 1 cut(s) 106
BoxI GACNNNNGTC 1 cut(s) 50
BpmI CTGGAG 2 cut(s) 216, 1198
Bpu10I CCTNAGC 2 cut(s) 21, 1090
BpuEI CTTGAG 1 cut(s) 171
BpuMI CCSGG 2 cut(s) 1200, 1249
BsaAI YACGTR 1 cut(s) 267
BsaBI GATNNNNATC 1 cut(s) 406
BsaJI CCNNGG 3 cut(s) 439, 662, 1248
BsaXI ACNNNNNCTCC 2 cut(s) 997, 1027
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 3 cut(s) 755, 1123, 1188
Bse8I GATNNNNATC 1 cut(s) 406
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 3 cut(s) 439, 662, 1248
BseGI GGATG 1 cut(s) 553
BseJI GATNNNNATC 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMII CTCAG 3 cut(s) 224, 392, 694
BseNI ACTGG 3 cut(s) 755, 1123, 1188
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 9 cut(s) 97, 106, 218, 436, 458, 530, 556, 741, 1123
BseYI CCCAGC 1 cut(s) 956
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 2 cut(s) 492, 777
BshNI GGYRCC 2 cut(s) 527, 1085
BsiHKCI CYCGRG 2 cut(s) 1001, 1042
BsiSI CCGG 3 cut(s) 689, 1199, 1248
BslI CCNNNNNNNGG 1 cut(s) 602
BsmAI GTCTC 1 cut(s) 1174
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 2 cut(s) 492, 777
BsoBI CYCGRG 2 cut(s) 1001, 1042
Bsp120I GGGCCC 1 cut(s) 490
Bsp1286I GDGCHC 1 cut(s) 494
Bsp143I GATC 5 cut(s) 401, 407, 535, 657, 1111
Bsp19I CCATGG 1 cut(s) 662
BspACI CCGC 1 cut(s) 512
BspANI GGCC 2 cut(s) 492, 777
BspCNI CTCAG 3 cut(s) 223, 391, 693
BspLI GGNNCC 7 cut(s) 279, 387, 491, 492, 493, 529, 1087
BspMAI CTGCAG 2 cut(s) 124, 454
BspPI GGATC 3 cut(s) 396, 530, 652
BspT107I GGYRCC 2 cut(s) 527, 1085
BsrFI RCCGGY 1 cut(s) 688
BsrI ACTGG 3 cut(s) 755, 1123, 1188
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 3 cut(s) 439, 662, 1248
BssMI GATC 5 cut(s) 401, 407, 535, 657, 1111
BssT1I CCWWGG 2 cut(s) 439, 662
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 2 cut(s) 106, 370
Bst6I CTCTTC 1 cut(s) 662
BstAPI GCANNNNNTGC 3 cut(s) 527, 569, 1145
BstBAI YACGTR 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 1096, 1124
BstDEI CTNAG 7 cut(s) 21, 210, 378, 680, 785, 810, 1090
BstDSI CCRYGG 1 cut(s) 662
BstF5I GGATG 1 cut(s) 553
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 5 cut(s) 404, 410, 538, 660, 1114
BstMAI GTCTC 1 cut(s) 1174
BstMBI GATC 5 cut(s) 401, 407, 535, 657, 1111
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 835
BstPAI GACNNNNGTC 1 cut(s) 50
BstSCI CCNGG 3 cut(s) 193, 1198, 1247
BstSFI CTRYAG 3 cut(s) 120, 450, 978
BstSLI GKGCMC 1 cut(s) 494
BstV1I GCAGC 9 cut(s) 97, 106, 218, 436, 458, 530, 556, 741, 1123
BstX2I RGATCY 3 cut(s) 401, 407, 657
BstXI CCANNNNNNTGG 1 cut(s) 1176
BstYI RGATCY 3 cut(s) 401, 407, 657
BsuRI GGCC 2 cut(s) 492, 777
BtgI CCRYGG 1 cut(s) 662
BtrI CACGTC 1 cut(s) 60
BtsCI GGATG 1 cut(s) 553
BtsI GCAGTG 2 cut(s) 1026, 1077
BtsIMutI CAGTG 3 cut(s) 386, 1026, 1077
Cac8I GCNNGC 2 cut(s) 1096, 1124
CaiI CAGNNNCTG 1 cut(s) 572
CfoI GCGC 1 cut(s) 27
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 2 cut(s) 490, 491
Csp6I GTAC 4 cut(s) 191, 436, 484, 1086
CspCI CAANNNNNGTGG 2 cut(s) 748, 783
CviAII CATG 4 cut(s) 395, 663, 832, 1030
CviQI GTAC 4 cut(s) 191, 436, 484, 1086
DdeI CTNAG 7 cut(s) 21, 210, 378, 680, 785, 810, 1090
DpnI GATC 5 cut(s) 403, 409, 537, 659, 1113
DpnII GATC 5 cut(s) 401, 407, 535, 657, 1111
Eam1104I CTCTTC 1 cut(s) 662
EarI CTCTTC 1 cut(s) 662
Eco130I CCWWGG 2 cut(s) 439, 662
Eco147I AGGCCT 1 cut(s) 777
Eco24I GRGCYC 1 cut(s) 494
Eco47III AGCGCT 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 776
Eco88I CYCGRG 2 cut(s) 1001, 1042
EcoO109I RGGNCCY 2 cut(s) 490, 491
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 439, 662
EcoT38I GRGCYC 1 cut(s) 494
ErhI CCWWGG 2 cut(s) 439, 662
FaeI CATG 4 cut(s) 398, 666, 835, 1033
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FatI CATG 4 cut(s) 394, 662, 831, 1029
Fnu4HI GCNGC 9 cut(s) 111, 120, 207, 447, 450, 519, 570, 755, 1137
FokI GGATG 1 cut(s) 540
FriOI GRGCYC 1 cut(s) 494
Fsp4HI GCNGC 9 cut(s) 111, 120, 207, 447, 450, 519, 570, 755, 1137
FspBI CTAG 4 cut(s) 284, 374, 440, 645
GlaI GCGC 1 cut(s) 26
GluI GCNGC 9 cut(s) 111, 120, 207, 447, 450, 519, 570, 755, 1137
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 2 cut(s) 216, 1198
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 2 cut(s) 492, 777
HapII CCGG 3 cut(s) 689, 1199, 1248
HhaI GCGC 1 cut(s) 27
Hin1II CATG 4 cut(s) 398, 666, 835, 1033
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 1124
HinfI GANTC 6 cut(s) 297, 345, 584, 1024, 1151, 1156
HpaII CCGG 3 cut(s) 689, 1199, 1248
HphI GGTGA 3 cut(s) 22, 139, 322
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 3 cut(s) 213, 947, 1116
Hpy188III TCNNGA 7 cut(s) 284, 405, 794, 889, 1003, 1130, 1169
Hpy8I GTNNAC 1 cut(s) 130
HpyAV CCTTC 6 cut(s) 58, 219, 223, 476, 552, 800
HpyCH4III ACNGT 2 cut(s) 106, 370
HpyCH4IV ACGT 3 cut(s) 59, 266, 719
HpyCH4V TGCA 7 cut(s) 57, 98, 122, 338, 452, 1019, 1139
HpyF3I CTNAG 7 cut(s) 21, 210, 378, 680, 785, 810, 1090
HpySE526I ACGT 3 cut(s) 59, 266, 719
Hsp92II CATG 4 cut(s) 398, 666, 835, 1033
HspAI GCGC 1 cut(s) 25
KpnI GGTACC 1 cut(s) 1089
Kzo9I GATC 5 cut(s) 401, 407, 535, 657, 1111
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 9 cut(s) 97, 106, 218, 436, 458, 530, 556, 741, 1123
LweI GCATC 1 cut(s) 106
MaeI CTAG 4 cut(s) 284, 374, 440, 645
MaeII ACGT 3 cut(s) 59, 266, 719
MaeIII GTNAC 2 cut(s) 173, 868
MalI GATC 5 cut(s) 403, 409, 537, 659, 1113
MboI GATC 5 cut(s) 401, 407, 535, 657, 1111
MboII GAAGA 6 cut(s) 227, 230, 679, 798, 934, 966
MflI RGATCY 3 cut(s) 401, 407, 657
MhlI GDGCHC 1 cut(s) 494
MluCI AATT 9 cut(s) 14, 150, 216, 672, 712, 770, 933, 1194, 1257
MlyI GAGTC 1 cut(s) 1165
MmeI TCCRAC 1 cut(s) 805
MnlI CCTC 8 cut(s) 191, 291, 367, 508, 556, 626, 788, 999
MseI TTAA 3 cut(s) 32, 708, 1193
MspA1I CMGCKG 2 cut(s) 449, 569
MspI CCGG 3 cut(s) 689, 1199, 1248
MspR9I CCNGG 3 cut(s) 195, 1200, 1249
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 1 cut(s) 195
NciI CCSGG 2 cut(s) 1200, 1249
NcoI CCATGG 1 cut(s) 662
NdeII GATC 5 cut(s) 401, 407, 535, 657, 1111
NlaIII CATG 4 cut(s) 398, 666, 835, 1033
NlaIV GGNNCC 7 cut(s) 279, 387, 491, 492, 493, 529, 1087
NmuCI GTSAC 1 cut(s) 868
NspI RCATGY 1 cut(s) 835
PceI AGGCCT 1 cut(s) 777
PciI ACATGT 1 cut(s) 831
PctI GAATGC 1 cut(s) 462
PfeI GAWTC 5 cut(s) 297, 345, 584, 1024, 1151
PkrI GCNGC 9 cut(s) 112, 121, 208, 448, 451, 520, 571, 756, 1138
PleI GAGTC 1 cut(s) 1164
PpsI GAGTC 1 cut(s) 1164
Ppu21I YACGTR 1 cut(s) 267
PscI ACATGT 1 cut(s) 831
PshAI GACNNNNGTC 1 cut(s) 50
Psp6I CCWGG 1 cut(s) 193
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 7 cut(s) 279, 387, 491, 492, 493, 529, 1087
PspOMI GGGCCC 1 cut(s) 490
PspPI GGNCC 2 cut(s) 490, 491
PstI CTGCAG 2 cut(s) 124, 454
PstNI CAGNNNCTG 1 cut(s) 572
PsuI RGATCY 3 cut(s) 401, 407, 657
PvuII CAGCTG 2 cut(s) 449, 569
RsaI GTAC 4 cut(s) 192, 437, 485, 1087
RsaNI GTAC 4 cut(s) 191, 436, 484, 1086
SaqAI TTAA 3 cut(s) 32, 708, 1193
SatI GCNGC 9 cut(s) 111, 120, 207, 447, 450, 519, 570, 755, 1137
Sau3AI GATC 5 cut(s) 401, 407, 535, 657, 1111
Sau96I GGNCC 2 cut(s) 490, 491
SchI GAGTC 1 cut(s) 1165
ScrFI CCNGG 3 cut(s) 195, 1200, 1249
SduI GDGCHC 1 cut(s) 494
SfaNI GCATC 1 cut(s) 106
SfcI CTRYAG 3 cut(s) 120, 450, 978
SmlI CTYRAG 1 cut(s) 186
SmoI CTYRAG 1 cut(s) 186
Sse9I AATT 9 cut(s) 14, 150, 216, 672, 712, 770, 933, 1194, 1257
SseBI AGGCCT 1 cut(s) 777
SsiI CCGC 1 cut(s) 512
SspI AATATT 1 cut(s) 861
SspMI CTAG 4 cut(s) 284, 374, 440, 645
StuI AGGCCT 1 cut(s) 777
StyD4I CCNGG 3 cut(s) 193, 1198, 1247
StyI CCWWGG 2 cut(s) 439, 662
TaaI ACNGT 2 cut(s) 106, 370
TaiI ACGT 3 cut(s) 62, 269, 722
TaqI TCGA 4 cut(s) 92, 137, 295, 1154
TasI AATT 9 cut(s) 14, 150, 216, 672, 712, 770, 933, 1194, 1257
TatI WGTACW 1 cut(s) 483
TfiI GAWTC 5 cut(s) 297, 345, 584, 1024, 1151
Tru1I TTAA 3 cut(s) 32, 708, 1193
Tru9I TTAA 3 cut(s) 32, 708, 1193
TscAI CASTG 3 cut(s) 386, 1026, 1084
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 9 cut(s) 110, 119, 206, 446, 449, 518, 569, 754, 1136
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 5 cut(s) 234, 337, 411, 761, 895
TspGWI ACGGA 1 cut(s) 490
TspRI CASTG 3 cut(s) 386, 1026, 1084
XapI RAATTY 3 cut(s) 14, 672, 1257
XbaI TCTAGA 1 cut(s) 283
XceI RCATGY 1 cut(s) 835
XmaJI CCTAGG 1 cut(s) 439
XspI CTAG 4 cut(s) 284, 374, 440, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.