Rh5DG374800

anion transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004686
Physical Location & Seq
Forward (+)
56983307 .. 56984635
1329 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG374800.1

Sequence Viewer

Length: 1329 bp
ATGACGATGGTGAAATTTCCTAAGCGCTACTTAATCGTCATATTGACCTTCATCTGCACGTCTGTTTGCTATATAGAACGGGTCGGGTTTTCGATTGCATATACTGTTGCTGCCGATGCTGCAGGGGTAAACCAATCGAGTAAAGGCACAATTCTTTCTACATTCTATTATGGTTACGCTTGTTCACAAGTACCTGGAGGTTGGGCAGCTCAGAAAATTGGGGGAAGGAAGGTTCTTCTTCTTTCATTTGTGTTATGGTCAGCTACGTGTGCATTAGTTCCTCTAGACCCCAATCGAATCTTTGTCTTGGTGATTGCTCGGTTGCTTGTGGGCGTTGCACAAGGATTCATTTTCCCCTCAATCCACACAGTTCTAGCTCAGTGGGTTCCACCACATGAAAGATCCAGATCTGTTTCTCTTACTACTTCTGGTATGTACCTAGGAGCAGCTGCAGGAATGCTTCTCCTTCCAAGCCTTGTAAAGTACAGGGGCCCCCAATCCGTATTTCTTGCTGAGGCAGCATTAGGTGCCATGTGGTCTTTGCTGTGGTTCAAGTATGCCAGTGATCCACCTTCCTCTCAACATCCAAAAGCAACAGCTGCTGGTTTTGGGGAATCTTTGTTGCCGACCAAAGGAAGCCAAAAGTTGAAAGTAGAGAATGGAGGAAGTTCCACTAGAACTGCCAAGATCCCATGGAAGAGAATTTTACTCAGTTTGCCAGTTTGGGCAATCGTGGTTAATAATTTCACGTTCCATTATGCTTTATACGTGTTGATGAACTGGCTGCCCACCTACTTTGAATTAGGCCTCCAACTTAGCCTTCAGGAAATGGGTTCTTCTAAGATGTTGCCGTATCTCAACATGTTTATTTTCTCAAATATAGGTGGGGTGGTAGCTGACCATTTAGTCACCAAGAGAATATTGTCTGTGACTAATACTCGCAAGTTCTTGAACACCATTGGTTTCATAGTTGCTTCTCTTGCGTTGATGGCAATTCCCATCTTCCGAACTTCTGCTGGGGCTGTCTTTTGTTCTTCTGTAGCTCTTGGTTTCTTGGCACTCGGGAGGGCTGGTTTTGCAGTGAATCACATGGATATTGCCCCGAGATATGCTGGTATTGTAATGGGAGTTTCAAACACTGCTGGTACCTTAGCTGGCATTATTGGAGTTGATCTGACTGGCAAGCTTCTTGAAGCTGCAAGAACTGCTGATTCGAGTCTTTCAAGTCCAGAGAGCTGGAGACTGGTGTTTTTAATTCCGGGTCTGCTCTGCGTTTTCAGTTCTTTTGTATTTTTACTATTCTCAACCGGGGAGAGAATTTTTGACTGA

Protein Analysis

442

Amino Acids

47.8

Weight (kDa)

9.73

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 17 - 389 4.4e-50 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 905
Acc65I GGTACC 1 cut(s) 1145
AccB1I GGYRCC 2 cut(s) 527, 1145
AclWI GGATC 3 cut(s) 396, 560, 682
AcsI RAATTY 3 cut(s) 14, 702, 1317
AcuI CTGAAG 1 cut(s) 806
AfaI GTAC 4 cut(s) 192, 437, 485, 1147
AfeI AGCGCT 1 cut(s) 26
AfiI CCNNNNNNNGG 1 cut(s) 632
AflIII ACRYGT 3 cut(s) 266, 768, 861
AgsI TTSAA 7 cut(s) 553, 649, 800, 952, 1134, 1193, 1224
AjiI CACGTC 1 cut(s) 60
AjnI CCWGG 1 cut(s) 193
AjuI GAANNNNNNNTTGG 4 cut(s) 489, 521, 804, 836
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
Alw26I GTCTC 1 cut(s) 1234
AlwI GGATC 3 cut(s) 396, 560, 682
AlwNI CAGNNNCTG 1 cut(s) 602
Ama87I CYCGRG 2 cut(s) 1061, 1102
Aor51HI AGCGCT 1 cut(s) 26
AoxI GGCC 2 cut(s) 490, 805
ApaI GGGCCC 1 cut(s) 494
ApeKI GCWGC 9 cut(s) 110, 119, 206, 446, 449, 518, 599, 784, 1196
ApoI RAATTY 3 cut(s) 14, 702, 1317
Asp718I GGTACC 1 cut(s) 1145
AspA2I CCTAGG 1 cut(s) 439
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 2 cut(s) 490, 491
AsuC2I CCSGG 2 cut(s) 1260, 1309
AsuHPI GGTGA 3 cut(s) 22, 322, 901
AvaI CYCGRG 2 cut(s) 1061, 1102
AvrII CCTAGG 1 cut(s) 439
BaeGI GKGCMC 1 cut(s) 494
BanI GGYRCC 2 cut(s) 527, 1145
BanII GRGCYC 1 cut(s) 494
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 9 cut(s) 97, 106, 218, 436, 458, 530, 586, 771, 1183
BccI CCATC 2 cut(s) 982, 1007
BceAI ACGGC 1 cut(s) 835
BciT130I CCWGG 1 cut(s) 195
BcnI CCSGG 2 cut(s) 1260, 1309
BcoDI GTCTC 1 cut(s) 1234
BfaI CTAG 4 cut(s) 284, 374, 440, 675
BfmI CTRYAG 3 cut(s) 120, 450, 1038
BfoI RGCGCY 1 cut(s) 28
BglII AGATCT 1 cut(s) 407
BisI GCNGC 9 cut(s) 111, 120, 207, 447, 450, 519, 600, 785, 1197
BlnI CCTAGG 1 cut(s) 439
BlsI GCNGC 9 cut(s) 112, 121, 208, 448, 451, 520, 601, 786, 1198
Bme1390I CCNGG 3 cut(s) 195, 1260, 1309
BmeT110I CYCGRG 2 cut(s) 1061, 1102
BmgBI CACGTC 1 cut(s) 60
BmgT120I GGNCC 2 cut(s) 490, 491
BmiI GGNNCC 6 cut(s) 387, 491, 492, 493, 529, 1147
BmrFI CCNGG 3 cut(s) 195, 1260, 1309
BmsI GCATC 1 cut(s) 106
BpmI CTGGAG 2 cut(s) 216, 1258
Bpu10I CCTNAGC 3 cut(s) 21, 513, 1150
BpuMI CCSGG 2 cut(s) 1260, 1309
BsaAI YACGTR 2 cut(s) 267, 769
BsaBI GATNNNNATC 1 cut(s) 406
BsaJI CCNNGG 3 cut(s) 439, 692, 1308
BsaXI ACNNNNNCTCC 2 cut(s) 1057, 1087
Bsc4I CCNNNNNNNGG 1 cut(s) 632
Bse1I ACTGG 5 cut(s) 561, 719, 785, 1183, 1248
Bse8I GATNNNNATC 1 cut(s) 406
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 3 cut(s) 439, 692, 1308
BseGI GGATG 1 cut(s) 583
BseJI GATNNNNATC 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 632
BseMII CTCAG 4 cut(s) 224, 392, 504, 724
BseNI ACTGG 5 cut(s) 561, 719, 785, 1183, 1248
BseSI GKGCMC 1 cut(s) 494
BseXI GCAGC 9 cut(s) 97, 106, 218, 436, 458, 530, 586, 771, 1183
BseYI CCCAGC 1 cut(s) 1016
BsgI GTGCAG 1 cut(s) 40
BshFI GGCC 2 cut(s) 492, 807
BshNI GGYRCC 2 cut(s) 527, 1145
BsiHKCI CYCGRG 2 cut(s) 1061, 1102
BsiSI CCGG 2 cut(s) 1259, 1308
BslI CCNNNNNNNGG 1 cut(s) 632
BsmAI GTCTC 1 cut(s) 1234
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 2 cut(s) 492, 807
BsoBI CYCGRG 2 cut(s) 1061, 1102
Bsp120I GGGCCC 1 cut(s) 490
Bsp1286I GDGCHC 1 cut(s) 494
Bsp143I GATC 5 cut(s) 401, 407, 565, 687, 1171
Bsp19I CCATGG 1 cut(s) 692
BspANI GGCC 2 cut(s) 492, 807
BspCNI CTCAG 4 cut(s) 223, 391, 505, 723
BspLI GGNNCC 6 cut(s) 387, 491, 492, 493, 529, 1147
BspMAI CTGCAG 2 cut(s) 124, 454
BspPI GGATC 3 cut(s) 396, 560, 682
BspT107I GGYRCC 2 cut(s) 527, 1145
BsrI ACTGG 5 cut(s) 561, 719, 785, 1183, 1248
BssECI CCNNGG 3 cut(s) 439, 692, 1308
BssMI GATC 5 cut(s) 401, 407, 565, 687, 1171
BssT1I CCWWGG 2 cut(s) 439, 692
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 2 cut(s) 106, 370
Bst6I CTCTTC 1 cut(s) 692
BstAPI GCANNNNNTGC 3 cut(s) 527, 599, 1205
BstBAI YACGTR 2 cut(s) 267, 769
BstC8I GCNNGC 2 cut(s) 1156, 1184
BstDEI CTNAG 8 cut(s) 21, 210, 378, 513, 710, 815, 840, 1150
BstDSI CCRYGG 1 cut(s) 692
BstF5I GGATG 1 cut(s) 583
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 5 cut(s) 404, 410, 568, 690, 1174
BstMAI GTCTC 1 cut(s) 1234
BstMBI GATC 5 cut(s) 401, 407, 565, 687, 1171
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 1 cut(s) 865
BstSCI CCNGG 3 cut(s) 193, 1258, 1307
BstSFI CTRYAG 3 cut(s) 120, 450, 1038
BstSLI GKGCMC 1 cut(s) 494
BstV1I GCAGC 9 cut(s) 97, 106, 218, 436, 458, 530, 586, 771, 1183
BstX2I RGATCY 3 cut(s) 401, 407, 687
BstXI CCANNNNNNTGG 1 cut(s) 1236
BstYI RGATCY 3 cut(s) 401, 407, 687
BsuRI GGCC 2 cut(s) 492, 807
BtgI CCRYGG 1 cut(s) 692
BtrI CACGTC 1 cut(s) 60
BtsCI GGATG 1 cut(s) 583
BtsI GCAGTG 2 cut(s) 1086, 1137
BtsIMutI CAGTG 4 cut(s) 386, 568, 1086, 1137
Cac8I GCNNGC 2 cut(s) 1156, 1184
CaiI CAGNNNCTG 1 cut(s) 602
CfoI GCGC 1 cut(s) 27
Cfr13I GGNCC 2 cut(s) 490, 491
Csp6I GTAC 4 cut(s) 191, 436, 484, 1146
CspCI CAANNNNNGTGG 2 cut(s) 778, 813
CviAII CATG 5 cut(s) 395, 532, 693, 862, 1090
CviQI GTAC 4 cut(s) 191, 436, 484, 1146
DdeI CTNAG 8 cut(s) 21, 210, 378, 513, 710, 815, 840, 1150
DpnI GATC 5 cut(s) 403, 409, 567, 689, 1173
DpnII GATC 5 cut(s) 401, 407, 565, 687, 1171
DrdI GACNNNNNNGTC 1 cut(s) 905
DseDI GACNNNNNNGTC 1 cut(s) 905
Eam1104I CTCTTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 692
Eco130I CCWWGG 2 cut(s) 439, 692
Eco147I AGGCCT 1 cut(s) 807
Eco24I GRGCYC 1 cut(s) 494
Eco47III AGCGCT 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 806
Eco88I CYCGRG 2 cut(s) 1061, 1102
EcoO109I RGGNCCY 2 cut(s) 490, 491
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 439, 692
EcoT38I GRGCYC 1 cut(s) 494
ErhI CCWWGG 2 cut(s) 439, 692
FaeI CATG 5 cut(s) 398, 535, 696, 865, 1093
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FatI CATG 5 cut(s) 394, 531, 692, 861, 1089
Fnu4HI GCNGC 9 cut(s) 111, 120, 207, 447, 450, 519, 600, 785, 1197
FokI GGATG 1 cut(s) 570
FriOI GRGCYC 1 cut(s) 494
Fsp4HI GCNGC 9 cut(s) 111, 120, 207, 447, 450, 519, 600, 785, 1197
FspBI CTAG 4 cut(s) 284, 374, 440, 675
GlaI GCGC 1 cut(s) 26
GluI GCNGC 9 cut(s) 111, 120, 207, 447, 450, 519, 600, 785, 1197
GsaI CCCAGC 1 cut(s) 1020
GsuI CTGGAG 2 cut(s) 216, 1258
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 2 cut(s) 492, 807
HapII CCGG 2 cut(s) 1259, 1308
HhaI GCGC 1 cut(s) 27
Hin1II CATG 5 cut(s) 398, 535, 696, 865, 1093
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HindIII AAGCTT 1 cut(s) 1184
HinfI GANTC 6 cut(s) 297, 345, 614, 1084, 1211, 1216
HpaII CCGG 2 cut(s) 1259, 1308
HphI GGTGA 3 cut(s) 22, 322, 901
Hpy166II GTNNAC 2 cut(s) 130, 185
Hpy188I TCNGA 3 cut(s) 213, 1007, 1176
Hpy188III TCNNGA 7 cut(s) 284, 405, 824, 949, 1063, 1190, 1229
Hpy8I GTNNAC 2 cut(s) 130, 185
HpyAV CCTTC 6 cut(s) 58, 219, 223, 476, 582, 830
HpyCH4III ACNGT 2 cut(s) 106, 370
HpyCH4IV ACGT 4 cut(s) 59, 266, 749, 768
HpyCH4V TGCA 8 cut(s) 57, 98, 122, 272, 338, 452, 1079, 1199
HpyF3I CTNAG 8 cut(s) 21, 210, 378, 513, 710, 815, 840, 1150
HpySE526I ACGT 4 cut(s) 59, 266, 749, 768
Hsp92II CATG 5 cut(s) 398, 535, 696, 865, 1093
HspAI GCGC 1 cut(s) 25
KpnI GGTACC 1 cut(s) 1149
Kzo9I GATC 5 cut(s) 401, 407, 565, 687, 1171
LmnI GCTCC 1 cut(s) 443
Lsp1109I GCAGC 9 cut(s) 97, 106, 218, 436, 458, 530, 586, 771, 1183
LweI GCATC 1 cut(s) 106
MaeI CTAG 4 cut(s) 284, 374, 440, 675
MaeII ACGT 4 cut(s) 59, 266, 749, 768
MaeIII GTNAC 3 cut(s) 173, 907, 928
MalI GATC 5 cut(s) 403, 409, 567, 689, 1173
MboI GATC 5 cut(s) 401, 407, 565, 687, 1171
MboII GAAGA 6 cut(s) 227, 230, 709, 828, 994, 1026
MflI RGATCY 3 cut(s) 401, 407, 687
MhlI GDGCHC 1 cut(s) 494
MluCI AATT 9 cut(s) 14, 150, 216, 702, 742, 800, 993, 1254, 1317
MlyI GAGTC 1 cut(s) 1225
MmeI TCCRAC 1 cut(s) 835
MnlI CCTC 8 cut(s) 191, 291, 367, 508, 586, 656, 818, 1059
MseI TTAA 3 cut(s) 32, 738, 1253
MspA1I CMGCKG 2 cut(s) 449, 599
MspI CCGG 2 cut(s) 1259, 1308
MspR9I CCNGG 3 cut(s) 195, 1260, 1309
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 1 cut(s) 195
NciI CCSGG 2 cut(s) 1260, 1309
NcoI CCATGG 1 cut(s) 692
NdeII GATC 5 cut(s) 401, 407, 565, 687, 1171
NlaIII CATG 5 cut(s) 398, 535, 696, 865, 1093
NlaIV GGNNCC 6 cut(s) 387, 491, 492, 493, 529, 1147
NmuCI GTSAC 2 cut(s) 907, 928
NspI RCATGY 1 cut(s) 865
PceI AGGCCT 1 cut(s) 807
PciI ACATGT 1 cut(s) 861
PctI GAATGC 1 cut(s) 462
PfeI GAWTC 5 cut(s) 297, 345, 614, 1084, 1211
PkrI GCNGC 9 cut(s) 112, 121, 208, 448, 451, 520, 601, 786, 1198
PleI GAGTC 1 cut(s) 1224
PpsI GAGTC 1 cut(s) 1224
Ppu21I YACGTR 2 cut(s) 267, 769
PscI ACATGT 1 cut(s) 861
Psp6I CCWGG 1 cut(s) 193
PspFI CCCAGC 1 cut(s) 1016
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 6 cut(s) 387, 491, 492, 493, 529, 1147
PspOMI GGGCCC 1 cut(s) 490
PspPI GGNCC 2 cut(s) 490, 491
PstI CTGCAG 2 cut(s) 124, 454
PstNI CAGNNNCTG 1 cut(s) 602
PsuI RGATCY 3 cut(s) 401, 407, 687
PvuII CAGCTG 2 cut(s) 449, 599
RsaI GTAC 4 cut(s) 192, 437, 485, 1147
RsaNI GTAC 4 cut(s) 191, 436, 484, 1146
SaqAI TTAA 3 cut(s) 32, 738, 1253
SatI GCNGC 9 cut(s) 111, 120, 207, 447, 450, 519, 600, 785, 1197
Sau3AI GATC 5 cut(s) 401, 407, 565, 687, 1171
Sau96I GGNCC 2 cut(s) 490, 491
SchI GAGTC 1 cut(s) 1225
ScrFI CCNGG 3 cut(s) 195, 1260, 1309
SduI GDGCHC 1 cut(s) 494
SfaNI GCATC 1 cut(s) 106
SfcI CTRYAG 3 cut(s) 120, 450, 1038
Sse9I AATT 9 cut(s) 14, 150, 216, 702, 742, 800, 993, 1254, 1317
SseBI AGGCCT 1 cut(s) 807
SspI AATATT 1 cut(s) 921
SspMI CTAG 4 cut(s) 284, 374, 440, 675
StuI AGGCCT 1 cut(s) 807
StyD4I CCNGG 3 cut(s) 193, 1258, 1307
StyI CCWWGG 2 cut(s) 439, 692
TaaI ACNGT 2 cut(s) 106, 370
TaiI ACGT 4 cut(s) 62, 269, 752, 771
TaqI TCGA 4 cut(s) 92, 137, 295, 1214
TasI AATT 9 cut(s) 14, 150, 216, 702, 742, 800, 993, 1254, 1317
TatI WGTACW 1 cut(s) 483
TfiI GAWTC 5 cut(s) 297, 345, 614, 1084, 1211
Tru1I TTAA 3 cut(s) 32, 738, 1253
Tru9I TTAA 3 cut(s) 32, 738, 1253
TscAI CASTG 4 cut(s) 386, 568, 1086, 1144
TseFI GTSAC 2 cut(s) 907, 928
TseI GCWGC 9 cut(s) 110, 119, 206, 446, 449, 518, 599, 784, 1196
Tsp45I GTSAC 2 cut(s) 907, 928
TspDTI ATGAA 6 cut(s) 40, 234, 337, 411, 791, 955
TspGWI ACGGA 1 cut(s) 490
TspRI CASTG 4 cut(s) 386, 568, 1086, 1144
XapI RAATTY 3 cut(s) 14, 702, 1317
XbaI TCTAGA 1 cut(s) 283
XceI RCATGY 1 cut(s) 865
XmaJI CCTAGG 1 cut(s) 439
XspI CTAG 4 cut(s) 284, 374, 440, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.